http://lod.bco-dmo.org/id/dataset/914498
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-11-06
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Montipora capitata and Porites compressa Physiological Measurements for Experimental Corals and Parent Colonies from 2014-2015 (RAPID Hawaii project)
2023-11-06
publication
2023-11-06
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-09-17
publication
https://doi.org/10.26008/1912/bco-dmo.914498.1
Andréa G. Grottoli
Ohio State University
principalInvestigator
Robert J. Toonen
University of Hawai'i
principalInvestigator
Kerri Dobson
Ohio State University
principalInvestigator
Christopher P. Jury
University of Hawai'i
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Grottoli, A. G., Toonen, R. J., Dobson, K., Jury, C. P. (2024) Montipora capitata and Porites compressa Physiological Measurements for Experimental Corals and Parent Colonies from 2014-2015 (RAPID Hawaii project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-11-06 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.914498.1 [access date]
Montipora capitata and Porites compressa Physiological Measurements for Experimental Corals and Parent Colonies Dataset Description: Methods and Sampling: <p><strong>Sampling Details</strong></p>
<p>Between 10–12 November 2014, three weeks following the peak of the 2014 thermal stress event, six visibly non-bleached and six visibly bleached parent colonies of <em>Montipora capitata</em> and <em>Porites compressa</em> were collected from 1–3m depth from the fringing reefs surrounding Moku o Loʻe in Kāneʻohe Bay (centered at 21°26’6” N, 157°47’12” W) and in Waimānalo Bay (centered at 21°19’36” N, 157°40’54” W) using a hammer and chisel.</p>
<p>One ramet from each colony was immediately placed in a -20 °C freezer to evaluate the initial physiology of the parent colonies prior to experimental manipulation. An additional twelve ramets were collected from each parent colony of each species from each location (totaling 576 fragments) and mounted on pre-labeled plastic tiles for use in the manipulative experiment.</p>
<p><strong>Experimental Tank Conditions</strong></p>
<p>All experimental ramets were returned to the Hawai‘i Institute of Marine Biology&nbsp;and allowed to recover under ambient seawater conditions in outdoor, unfiltered, flow-through seawater tanks for three days. Natural light levels were reduced using 40% neutral density shade cloth such that photosynthetically active radiation levels mimicked that at collection depth.</p>
<p>On 15 November 2014, ramets were equally divided among eight outdoor, 38 L experimental tanks supplied with unfiltered, flow-through seawater. Though the incoming seawater was unfiltered, virtually no zooplankton is delivered to experimental tanks at HIMB due to scouring of the inflowing seawater by in-line invertebrates. Two experimental tanks were randomly assigned to each of the following four treatments: 1) Control (ambient seawater of pH 7.97 and unfed), 2) Fed (ambient seawater of pH 7.97 and fed), 3) OA (acidified seawater of pH 7.74 and unfed), and 4) OA+Fed (acidified seawater pH 7.74 and fed).</p>
<p>Coral holobiont physiology was assessed for parent colonies (i.e., in situ reef controls) and a subset of experimental corals in December 2014 (2 months after 2014 peak thermal stress; 1 month in experimental tanks), June 2015 (8 months after 2014 peak thermal stress; 7 months in experimental tanks), and November 2015 (one month after 2015 peak thermal stress; 13 months in experimental tanks).&nbsp;At each experimental time point, physiological trait measurements were conducted (calcification, photosynthesis, respiration, and zooplankton feeding rate capacity). Once all live measurements were completed, these ramets were frozen for further analyses (endosymbiont cell density, tissue biomass, and total lipids).</p>
<p>The seawater pH was reduced in the simulated OA treatments by bubbling CO gas into the tanks. To ensure tanks remained within target pH range, all tanks were spot-checked with a calibrated pH probe every 1–2 days throughout the experiment. This data was recorded during periods when physiological measurements were being made. Small adjustments to the CO2 bubbling rate were made as needed to maintain the desired 0.2 pH offset between ambient and simulated OA treatments.</p>
<p>Weekly, pH and total alkalinity measurements were made on seawater samples collected from each tank at18:00 h. Total alkalinity was measured using a modified Gran titration and checked for accuracy and precision (~5 μmol kg) using certified reference materials obtained from A. Dickson (Scripps Institution of Oceanography). pH was measured spectrophotometrically at 25 °C with m-cresol purple (precision ~0.002 pH units).</p>
<p>These parameters were then entered into CO2Sys to calculate pH in situ. The CO bubbling rate was then adjusted as necessary, pH was measured again using a calibrated pH probe, and any further adjustments were made until the desired pH was consistently read. In December 2014, March 2015, and June2015, diel cycle chemistry was measured over a 24-hour period across all tanks using the same method.</p>
<p>&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1514859 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1514859
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1514861 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1514861
completed
Andréa G. Grottoli
Ohio State University
614-292-5782
School of Earth Sciences 125 South Oval Mall
Columbus
OH
43210
United States
grottoli.1@osu.edu
pointOfContact
Robert J. Toonen
University of Hawai'i
808-236-7401
Hawaii Institute of Marine Biology 46-007 Lilipuna Rd
Kaneohe
HI
96744
USA
rjtoonen@gmail.com
pointOfContact
Kerri Dobson
Ohio State University
+1-614-620-9772
School of Earth Sciences 125 South Oval Mall
Columbus
OH
43210
USA
K.L.Dobson@soton.ac.uk
pointOfContact
Christopher P. Jury
University of Hawai'i
808-836-7425
jurycp@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Species
Collection_site
Lat
Long
Colony
Sample_ID
Health_status
pH
Feeding_status
Treatment
Tank
Time_point
Endo_density
Calc
Total_biomass
Total_lipids
Gross_P
LEDR
CZAR
FR
CHAR
CTAR
YSI ProODO Optical DIssolved Oxygen
Thermo Scientific Genesys
theme
None, User defined
species
site
latitude
longitude
sample description
sample identification
treatment
tank
time_point
density
calcification
wet_weight_biomass
total lipids
photosynthetic rate
respiration rate
food consumption
featureType
BCO-DMO Standard Parameters
Oxygen Sensor
Spectrophotometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Will corals recover from bleaching under ocean acidification conditions?
https://osprey.bco-dmo.org/project/546319
Will corals recover from bleaching under ocean acidification conditions?
<p>Following the second hottest month on record since the 1940s, water temperatures on O'ahu reached 30 degrees C. The result of this ~2 degree C increase above summer mean temperatures has been a severe bleaching event across the entire length of the Hawaiian Archipelago, with as many as 75% of the dominant coral species in Kane'ohe Bay losing color or bleaching completely white. This event exceeds the magnitude of the only major bleaching event previously documented for Hawaii in 1996. Although tragic, this event provides a rare natural experiment to understand the impact of coral bleaching on the ability of Hawaiian corals to recovery from high temperature stress in the context of climate change and ocean acidification. The proposed will leverage previous work by the PIs to compare recovery following this event and the 1996 mass bleaching event to the recovery rates of Hawaiian corals under future climate change scenarios. Results from this work will provide data on coral resistance and recovery potential from bleaching events of the future.</p>
<p>Coral reefs are among the most diverse ecosystems on the planet, housing an estimated 25% of marine species. But, that diversity appears particularly susceptible to the effects of global change. Massive coral bleaching poses a substantial threat to the integrity of coral reef habitat in US waters, and is predicted to be the major source of mortality for reefs under future climate scenarios. Although previous work on the recovery of corals from bleaching sets the groundwork for this project, it remains to be seen how recovery from bleaching will be impacted by climate change and ocean acidification. To address this fundamental question, we take advantage of the natural difference in baseline temperature and <em>p</em>CO2 conditions between Kane'ohe Bay and Waimanalo Bay, HI, both of which are currently impacted by the massive bleaching event in the Hawaiian Archipelago. This natural experiment makes possible a rare opportunity to test three basic questions about the rates of recovery of bleached and unbleached corals under future climate change scenarios:<br />
1) Will ocean acidification slow rates of recovery from bleaching?;<br />
2) Does zooplankton feeding minimize the impact?; and<br />
3) Do corals acclimated to warmer, more acidic baseline conditions (Kane'ohe Bay) recover more quickly under future conditions than corals from present day mean oceanic conditions (Waimanalo Bay)?</p>
<p>This research addresses broad scientific questions relating to the ability of corals to acclimate or adapt to both local environments and future climate conditions, and to help identify coral populations that may be resilient to the predicted impacts of climate change on the reefs of the future.</p>
RAPID Hawaii
largerWorkCitation
project
eng; USA
oceans
-157.786667
-157.681667
21.326667
21.435
2014-11-01
2015-12-31
Oahu, HI; Hawaii Institute of Marine Biology
0
BCO-DMO catalogue of parameters from Montipora capitata and Porites compressa Physiological Measurements for Experimental Corals and Parent Colonies from 2014-2015 (RAPID Hawaii project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/914630.rdf
Name: Species
Units: unitless
Description: <p>Species of parent colony from which samples were collected. MC = Montipora capitata; PC = Porites compressa.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914631.rdf
Name: Collection_site
Units: unitless
Description: <p>Collection site representing the location of the parent colony from which samples were collected. KB = K_ne_ohe Bay; W = Waim_nalo Bay.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914634.rdf
Name: Lat
Units: decimal degrees
Description: <p>Collection site latitude in degrees, minutes, seconds.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914635.rdf
Name: Long
Units: decimal degrees
Description: <p>Collection site longitude in degrees, minutes, seconds.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914636.rdf
Name: Colony
Units: unitless
Description: <p>Colony identification number.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914637.rdf
Name: Sample_ID
Units: unitless
Description: <p>Unique sample identification number.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914638.rdf
Name: Health_status
Units: unitless
Description: <p>Health status of parent colony. NB = non-bleached; B = bleached.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914639.rdf
Name: pH
Units: unitless
Description: <p>Seawater pH of experimental tanks. AMB = ambient seawater pH (7.97); OA = acidified seawater / low pH (7.74).</p>
http://lod.bco-dmo.org/id/dataset-parameter/914640.rdf
Name: Feeding_status
Units: unitless
Description: <p>Feeding status of experimental tank. NF = not fed; F = fed.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914641.rdf
Name: Treatment
Units: unitless
Description: <p>Treatment or parent colony status of experimental sample. Parent colony = parent colony; BL AMB F = bleached, ambient pH, fed; BL AMB NF = bleached, ambient pH, not fed; BL OA F = bleached, low pH, fed; BL OA NF = bleached, low pH, not fed; NB AMB F = non-bleached.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914642.rdf
Name: Tank
Units: unitless
Description: <p>Tank identification number.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914643.rdf
Name: Time_point
Units: unitless
Description: <p>Collection time for Parent colony and time of removal from experiment for those in treatment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914644.rdf
Name: Endo_density
Units: cells cm-2
Description: <p>Endosymbiont cell density of coral sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914645.rdf
Name: Calc
Units: mg day-1 cm-2
Description: <p>Calcification of coral sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914646.rdf
Name: Total_biomass
Units: g cm-2
Description: <p>Total biomass of sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914647.rdf
Name: Total_lipids
Units: J gdw-1
Description: <p>Total lipids.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914648.rdf
Name: Gross_P
Units: umol O2 min-1 cm-2
Description: <p>Gross photosynthesis.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914649.rdf
Name: LEDR
Units: unitless
Description: <p>Light enhanced dark respiration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914650.rdf
Name: CZAR
Units: unitless
Description: <p>Contribution (percentage) of Zooxanthellae (Symbiodiniaceae) to Animal Respiration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914651.rdf
Name: FR
Units: Brine shrimp hr-1 cm-2
Description: <p>Feeding rate.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914652.rdf
Name: CHAR
Units: unitless
Description: <p>Contribution (percentage) of heterotrophy to animal respiration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/914653.rdf
Name: CTAR
Units: unitless
Description: <p>Contribution (percentage) of total fixed carbon to animal respiration.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
103132
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/6bd3c225-57fe-4736-b801-d77c37fa282f/content
download
https://doi.org/10.26008/1912/bco-dmo.914498.1
download
onLine
dataset
<p><strong>Sampling Details</strong></p>
<p>Between 10–12 November 2014, three weeks following the peak of the 2014 thermal stress event, six visibly non-bleached and six visibly bleached parent colonies of <em>Montipora capitata</em> and <em>Porites compressa</em> were collected from 1–3m depth from the fringing reefs surrounding Moku o Loʻe in Kāneʻohe Bay (centered at 21°26’6” N, 157°47’12” W) and in Waimānalo Bay (centered at 21°19’36” N, 157°40’54” W) using a hammer and chisel.</p>
<p>One ramet from each colony was immediately placed in a -20 °C freezer to evaluate the initial physiology of the parent colonies prior to experimental manipulation. An additional twelve ramets were collected from each parent colony of each species from each location (totaling 576 fragments) and mounted on pre-labeled plastic tiles for use in the manipulative experiment.</p>
<p><strong>Experimental Tank Conditions</strong></p>
<p>All experimental ramets were returned to the Hawai‘i Institute of Marine Biology&nbsp;and allowed to recover under ambient seawater conditions in outdoor, unfiltered, flow-through seawater tanks for three days. Natural light levels were reduced using 40% neutral density shade cloth such that photosynthetically active radiation levels mimicked that at collection depth.</p>
<p>On 15 November 2014, ramets were equally divided among eight outdoor, 38 L experimental tanks supplied with unfiltered, flow-through seawater. Though the incoming seawater was unfiltered, virtually no zooplankton is delivered to experimental tanks at HIMB due to scouring of the inflowing seawater by in-line invertebrates. Two experimental tanks were randomly assigned to each of the following four treatments: 1) Control (ambient seawater of pH 7.97 and unfed), 2) Fed (ambient seawater of pH 7.97 and fed), 3) OA (acidified seawater of pH 7.74 and unfed), and 4) OA+Fed (acidified seawater pH 7.74 and fed).</p>
<p>Coral holobiont physiology was assessed for parent colonies (i.e., in situ reef controls) and a subset of experimental corals in December 2014 (2 months after 2014 peak thermal stress; 1 month in experimental tanks), June 2015 (8 months after 2014 peak thermal stress; 7 months in experimental tanks), and November 2015 (one month after 2015 peak thermal stress; 13 months in experimental tanks).&nbsp;At each experimental time point, physiological trait measurements were conducted (calcification, photosynthesis, respiration, and zooplankton feeding rate capacity). Once all live measurements were completed, these ramets were frozen for further analyses (endosymbiont cell density, tissue biomass, and total lipids).</p>
<p>The seawater pH was reduced in the simulated OA treatments by bubbling CO gas into the tanks. To ensure tanks remained within target pH range, all tanks were spot-checked with a calibrated pH probe every 1–2 days throughout the experiment. This data was recorded during periods when physiological measurements were being made. Small adjustments to the CO2 bubbling rate were made as needed to maintain the desired 0.2 pH offset between ambient and simulated OA treatments.</p>
<p>Weekly, pH and total alkalinity measurements were made on seawater samples collected from each tank at18:00 h. Total alkalinity was measured using a modified Gran titration and checked for accuracy and precision (~5 μmol kg) using certified reference materials obtained from A. Dickson (Scripps Institution of Oceanography). pH was measured spectrophotometrically at 25 °C with m-cresol purple (precision ~0.002 pH units).</p>
<p>These parameters were then entered into CO2Sys to calculate pH in situ. The CO bubbling rate was then adjusted as necessary, pH was measured again using a calibrated pH probe, and any further adjustments were made until the desired pH was consistently read. In December 2014, March 2015, and June2015, diel cycle chemistry was measured over a 24-hour period across all tanks using the same method.</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Differences in the physiological profiles (including endosymbiont cell density, CTAR, calcification, tissue biomass, and total lipids) between species, sites, and sites within species was assessed with a multivariate analysis of similarity (ANOSIM).</p>
<p>Physiological profiles of experimental ramets in December 2014 and June 2015 were evaluated using both multivariate and univariate analyses.</p>
<p>Additional details of statistical analyses are outlined in the associated results publication (Dobson et al.).</p>
Specified by the Principal Investigator(s)
- Time_point (date) field converted from %d-%y (e.g., Nov-14) to %m-%Y (e.g., 11-2014) format
- Converted latitude and longitude fields from degrees, minutes, seconds to decimal degree format, and rounded these fields to six degrees of precision
- Empty cells represent "No data" within the dataset
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
YSI ProODO Optical DIssolved Oxygen
YSI ProODO Optical DIssolved Oxygen
PI Supplied Instrument Name: YSI ProODO Optical DIssolved Oxygen PI Supplied Instrument Description:YSI ProODO Optical Dissolved Oxygen probes were used to measure dissolved oxygen during coral incubations for use in the calculation of photosynthesis and respiration rates. Instrument Name: Oxygen Sensor Instrument Short Name:Dissolved Oxygen Sensor Instrument Description: An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed
Thermo Scientific Genesys
Thermo Scientific Genesys
PI Supplied Instrument Name: Thermo Scientific Genesys PI Supplied Instrument Description:A Thermo Scientific Genesys spectrophotometer was used to measure the intensity of light transmission at specific wavelengths for chlorophyll a. Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/