http://lod.bco-dmo.org/id/dataset/914723
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-11-09
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Photochemical yield and bleaching severity data from a standardized short-term (18 hour) heat stress assay of Montipora capitata and Porites compressa collected at a patch reef in Kane'ohe Bay, O'ahu, Hawai'i in September of 2022
2023-11-09
publication
2023-11-09
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-11-28
publication
https://doi.org/10.26008/1912/bco-dmo.914723.1
Katie Barott
University of Pennsylvania
principalInvestigator
Hollie Putnam
University of Rhode Island
principalInvestigator
Kristen Brown
University of Pennsylvania
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Barott, K., Putnam, H., Brown, K. T. (2023) Photochemical yield and bleaching severity data from a standardized short-term (18 hour) heat stress assay of Montipora capitata and Porites compressa collected at a patch reef in Kane'ohe Bay, O'ahu, Hawai'i in September of 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-11-09 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.914723.1 [access date]
Dataset Description: <p>This dataset and other data from this study will be published in the results paper "Divergent bleaching and recovery trajectories in reef-building corals following a decade of successive marine heatwaves." (see pre-print Brown, et al. (2023), doi: 10.1101/2023.07.16.549193).&nbsp;<br />
<br />
All BCO-DMO datasets related to this publication can be found on the page https://www.bco-dmo.org/related-resource/915300.<br />
<br />
See Related Datasets section for "Short-term heat stress assay: Temperature data" from the same short-term (18 hour) heat stress assay.</p> Methods and Sampling: <div><span style="font-size:13px">Sampling Location: patch reef 13 in Kāne'ohe Bay, O'ahu, Hawai'i (21.4509, -157.7954)</span></div>
<div><br />
The following methods describe how this dataset was produced along with the related dataset "<span style="font-size:13px">Short-term heat stress assay: Temperature data".</span><br />
&nbsp;</div>
<div>
<div><span style="font-size:13px">The following methods describe how this dataset was produced along with the related dataset "Short-term heat stress assay: temperature data" (https://www.bco-dmo.org/dataset/914715)</span></div>
<div>&nbsp;</div>
<div><span style="font-size:13px">Acute heat stress experiment</span></div>
<div><span style="font-size:13px">Ten individual colonies of each phenotype per species were sampled on September 1, 2022. Four fragments were collected from each individual colony (i.e., genetic clones) by hand using bone cutters, totalling 160 fragments. Following collection, corals were transported to the Hawaiʻi Institute of Marine Biology (HIMB) and placed in outdoor, flow-through seawater tanks under ambient temperatures (mean ± SD; 28.2 ± 0.1°C, n=2 tanks) until experimentation. Coral thermal tolerance experiments were initiated 72 hours after collection. The experimental heat stress assay followed the standardized temperature profile used to measure heat tolerance in corals (e.g., MMM, MMM+3°C, MMM+6°C, and MMM+9°C) (Voolstra, et al., 2020). The experiment began on September 4 2022 at 13:00 with a 3-hour ramp to respective treatment temperatures (27.5°C, 30.5°C, 33.5°C, 36.5°C), a 3-hour hold, and a 1-hour ramp down to MMM temperature (27.5°C). A fragment from each coral colony was suspended using fishing line and randomly placed into each treatment, so that all genotypes across species were present in each treatment. Temperatures were controlled using a custom computer controlled system (Raspberry Pi). Temperatures were constantly monitored and manipulated by responding to probe measurements in real time, and recorded using cross-calibrated temperature loggers (accuracy: ± 0.47°C at 25°C; resolution: ± 0.10°C at 25°C HOBO UA-001-64, Onset Computer Corporation). Experiments were performed outdoors under natural photosynthetically active radiation (PAR), which ranged between 515–580 µmol m-2 sec-1 at midday (~12:00; Licor cosine sensor). At the end of the ramp and 1 h after sunset (~19:30), corals were assessed for dark-adapted photochemical yield (Fv/Fm) using a Diving-PAM (Walz GmbH) 5-mm diameter fiber-optic probe at a standardized distance (5 mm) above the coral tissue after F0 stabilized. Three random spots (2–3 cm apart across the front and back of the nubbin) were measured on each fragment to obtain average measures of Fv/Fm. All readings with F0 values that were less than 110 were removed to avoid any false detections (Marzonie et al., 2022). The following morning at 07:00 corals were photographed with a color standard (WDKK Waterproof Color Chart, DGK Color Tools) to assess coral color, a proxy for bleaching severity. Bleaching severity was determined visually (e.g., 0, 20, 40, 60, 80, 100% white) (Evensen, et al.., 2022) using the six gray standards as a reference (e.g., black= 0% bleached, white = 100% bleached) from two photographs (front and back of the fragment) to obtain an average color score (Brown et al., 2023 Fig. S4).</span></div>
&nbsp;
<div>Taxonomic Identifiers:</div>
<div><span style="font-size:13px">Montipora capitata, LSID (urn:lsid:marinespecies.org:taxname:287697)</span></div>
<div><span style="font-size:13px">Porites compressa, LSID (urn:lsid:marinespecies.org:taxname:207236)</span></div>
</div>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2102989 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2102989
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2103067 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2103067
completed
Katie Barott
University of Pennsylvania
215-746-6911
433 South University Avenue Leidy Laboratories 326
Philadelphia
PA
19104
USA
kbarott@sas.upenn.edu
pointOfContact
Hollie Putnam
University of Rhode Island
808-554-1462
120 Flagg Rd.
Kingston
RI
02881
USA
hputnam@uri.edu
pointOfContact
Kristen Brown
University of Pennsylvania
433 S University Ave
Philadelphia
PA
19104-4544
USA
ktbrown@sas.upenn.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Treatment
Temperature
Species
Bleach
Colony_ID
Rep
F
Y
M
Diving-PAM (Walz GmbH)
theme
None, User defined
treatment
temperature of sample or a generic measurement (not in-situ water temp)
species scientific name (binomial)
sample description
sample identification
replicate
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Fluorometer
Spectrometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RAPID: Collaborative Research: Disentangling the effects of heat stress versus bleaching phenotype on coral performance
https://osprey.bco-dmo.org/project/868513
RAPID: Collaborative Research: Disentangling the effects of heat stress versus bleaching phenotype on coral performance
<p><em>NSF Award Abstract:</em><br />
Coral bleaching has become increasingly common on reefs worldwide as rising sea surface temperatures associated with climate change disrupt the coral-algal symbiosis. This dramatic heat stress response turns the normally colorful corals bright white, and yet during these heat stress events not all corals undergo bleaching. This project focuses on assessing the effects of bleaching by comparing pairs of corals side-byside on the reef during an ongoing heat wave, where one has bleached and the other has not, despite experiencing the same temperatures. These coral pairs have been monitored throughout three bleaching events in the past five years, providing a unique resource to address whether corals with consistently different bleaching susceptibilities have the capacity to acclimate in response to disturbances through epigenetic changes, or changes in gene expression not due to change in DNA bases. To address this, the project will characterize the impacts of bleaching or not bleaching on coral physiology, gene expression, and epigenetic patterns using coral pairs in their natural habitat during a marine heatwave. This project also provides research support for graduate student trainees, as well as data and materials for the research and training of undergraduate and high school students. This project will recruit underrepresented minority students from URI and UPenn area high schools and university undergraduates for work on computer analysis of images (benthic and colony photographs, brightfield and confocal micrographs) and sequencing data. It will also support the training of an undergraduate student at the University of Hawaiʻi in coral ecology and physiology, and the development of her senior thesis.</p>
<p>This project will investigate the effects of repeated heat stress events on the performance of Montipora capitata, a dominant reef builder throughout Hawaiʻi. It utilizes the timely context of paired colonies of M. capitata with bleached vs. unbleached histories that have been monitored through two past bleaching events in Hawaiʻi (2015 and 2019) and the currently ongoing 2020 event. This system allows for the unique opportunity to disentangle the consequences of heat stress versus bleaching on coral performance through time, an essential feature of reef resilience. The contrasting physiological and energetic processes these two phenotypes undergo during a heatwave are likely to result in alterations to the cellular environment within the animal that impacts epigenetic transcriptional regulation. These regulatory and energetic changes, if persistent over time, have the potential to alter coral fitness beyond the duration of the heatwave differentially between corals with contrasting bleaching phenotypes. Specifically, the project will: 1) quantify the effect of the 2020 heatwave on coral physiology during bleaching and recovery, 2) generate a corresponding archive of coral tissues and nucleic acids as a resource for future work characterizing how bleaching phenotype alters energetics and non-genetic inheritance, and 3) characterize how bleaching phenotype alters intra-generational inheritance of epigenetic marks (i.e., DNA methylation) and gene expression, and the duration of these marks and expression patterns following heat stress. This project represents an urgent assessment of an ideal system to test the legacy of coral bleaching phenotype on coral fitness. The results of this project will therefore lay the foundation for intra and cross-generational effects of bleaching vs. heat stress, which is essential for understanding coral resilience to climate change.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Mcap pairs time series
largerWorkCitation
project
eng; USA
oceans
-157.7954
-157.7954
21.4509
21.4509
2022-09-01
2022-09-05
Coral reefs of Kaneohe Bay, Oahu, Hawaii
0
BCO-DMO catalogue of parameters from Photochemical yield and bleaching severity data from a standardized short-term (18 hour) heat stress assay of Montipora capitata and Porites compressa collected at a patch reef in Kane'ohe Bay, O'ahu, Hawai'i in September of 2022
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/914801.rdf
Name: Treatment
Units: unitless
Description: <p>Treatment identifier (AMB=ambient; or 3C, 6C, 9C)</p>
http://lod.bco-dmo.org/id/dataset-parameter/914802.rdf
Name: Temperature
Units: degrees Celcius
Description: <p>Temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/914803.rdf
Name: Species
Units: unitless
Description: <p>Species (Genus species)</p>
http://lod.bco-dmo.org/id/dataset-parameter/914804.rdf
Name: Bleach
Units: unitless
Description: <p>Bleach (phenotype)</p>
http://lod.bco-dmo.org/id/dataset-parameter/914805.rdf
Name: Colony_ID
Units: unitless
Description: <p>Colony identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/914806.rdf
Name: Rep
Units: unitless
Description: <p>replicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/914807.rdf
Name: F
Units: unitless
Description: <p>minimum fluorescence value</p>
http://lod.bco-dmo.org/id/dataset-parameter/914808.rdf
Name: Y
Units: unitless
Description: <p>photochemical yield</p>
http://lod.bco-dmo.org/id/dataset-parameter/914809.rdf
Name: M
Units: unitless
Description: <p>maximum amount of fluorescence</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
26802
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/3c673b2c-9e33-40d8-8158-27d5c026e72c/content
download
https://doi.org/10.26008/1912/bco-dmo.914723.1
download
onLine
dataset
<div><span style="font-size:13px">Sampling Location: patch reef 13 in Kāne'ohe Bay, O'ahu, Hawai'i (21.4509, -157.7954)</span></div>
<div><br />
The following methods describe how this dataset was produced along with the related dataset "<span style="font-size:13px">Short-term heat stress assay: Temperature data".</span><br />
&nbsp;</div>
<div>
<div><span style="font-size:13px">The following methods describe how this dataset was produced along with the related dataset "Short-term heat stress assay: temperature data" (https://www.bco-dmo.org/dataset/914715)</span></div>
<div>&nbsp;</div>
<div><span style="font-size:13px">Acute heat stress experiment</span></div>
<div><span style="font-size:13px">Ten individual colonies of each phenotype per species were sampled on September 1, 2022. Four fragments were collected from each individual colony (i.e., genetic clones) by hand using bone cutters, totalling 160 fragments. Following collection, corals were transported to the Hawaiʻi Institute of Marine Biology (HIMB) and placed in outdoor, flow-through seawater tanks under ambient temperatures (mean ± SD; 28.2 ± 0.1°C, n=2 tanks) until experimentation. Coral thermal tolerance experiments were initiated 72 hours after collection. The experimental heat stress assay followed the standardized temperature profile used to measure heat tolerance in corals (e.g., MMM, MMM+3°C, MMM+6°C, and MMM+9°C) (Voolstra, et al., 2020). The experiment began on September 4 2022 at 13:00 with a 3-hour ramp to respective treatment temperatures (27.5°C, 30.5°C, 33.5°C, 36.5°C), a 3-hour hold, and a 1-hour ramp down to MMM temperature (27.5°C). A fragment from each coral colony was suspended using fishing line and randomly placed into each treatment, so that all genotypes across species were present in each treatment. Temperatures were controlled using a custom computer controlled system (Raspberry Pi). Temperatures were constantly monitored and manipulated by responding to probe measurements in real time, and recorded using cross-calibrated temperature loggers (accuracy: ± 0.47°C at 25°C; resolution: ± 0.10°C at 25°C HOBO UA-001-64, Onset Computer Corporation). Experiments were performed outdoors under natural photosynthetically active radiation (PAR), which ranged between 515–580 µmol m-2 sec-1 at midday (~12:00; Licor cosine sensor). At the end of the ramp and 1 h after sunset (~19:30), corals were assessed for dark-adapted photochemical yield (Fv/Fm) using a Diving-PAM (Walz GmbH) 5-mm diameter fiber-optic probe at a standardized distance (5 mm) above the coral tissue after F0 stabilized. Three random spots (2–3 cm apart across the front and back of the nubbin) were measured on each fragment to obtain average measures of Fv/Fm. All readings with F0 values that were less than 110 were removed to avoid any false detections (Marzonie et al., 2022). The following morning at 07:00 corals were photographed with a color standard (WDKK Waterproof Color Chart, DGK Color Tools) to assess coral color, a proxy for bleaching severity. Bleaching severity was determined visually (e.g., 0, 20, 40, 60, 80, 100% white) (Evensen, et al.., 2022) using the six gray standards as a reference (e.g., black= 0% bleached, white = 100% bleached) from two photographs (front and back of the fragment) to obtain an average color score (Brown et al., 2023 Fig. S4).</span></div>
&nbsp;
<div>Taxonomic Identifiers:</div>
<div><span style="font-size:13px">Montipora capitata, LSID (urn:lsid:marinespecies.org:taxname:287697)</span></div>
<div><span style="font-size:13px">Porites compressa, LSID (urn:lsid:marinespecies.org:taxname:207236)</span></div>
</div>
Specified by the Principal Investigator(s)
<p>See results publication&nbsp;Brown, et al. (2023) for&nbsp;more detailed information on analysis and results.</p>
Specified by the Principal Investigator(s)
* Table within file "PAM data for STHS.csv " was imported into the BCO-DMO data system with values "NA" as missing data values.
** Missing data values are displayed differently based on the file format you download. They are blank in csv files, "NaN" in MatLab files, etc.
* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores. Can not start with a number]
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Diving-PAM (Walz GmbH)
Diving-PAM (Walz GmbH)
PI Supplied Instrument Name: Diving-PAM (Walz GmbH) PI Supplied Instrument Description:Diving-PAM (Walz GmbH) is an Underwater Fluorometer with Miniature Spectrometer.
Pulse-amplitude modulated (PAM) fluorometry.
Photochemical yield (Fv/Fm). Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
PI Supplied Instrument Name: PI Supplied Instrument Description:Diving-PAM (Walz GmbH) is an Underwater Fluorometer with Miniature Spectrometer. Instrument Name: Spectrometer Instrument Short Name:Spectrometer Instrument Description: A spectrometer is an optical instrument used to measure properties of light over a specific portion of the electromagnetic spectrum. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0460/