http://lod.bco-dmo.org/id/dataset/914845
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-11-13
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Pigment concentrations determined by HPLC from samples collected on Leg 2 (Hilo, HI to Papeete, French Polynesia) of the US GEOTRACES Pacific Meridional Transect (PMT) cruise (GP15, RR1815) on R/V Roger Revelle from Oct-Nov 2018
2023-11-13
publication
2023-11-13
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-11-17
publication
https://doi.org/10.26008/1912/bco-dmo.914845.1
Gregory A. Cutter
Old Dominion University
principalInvestigator
Karen L. Casciotti
Stanford University
principalInvestigator
Phoebe J. Lam
University of California-Santa Cruz
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Cutter, G. A., Casciotti, K. L., Lam, P. J. (2023) Pigment concentrations determined by HPLC from samples collected on Leg 2 (Hilo, HI to Papeete, French Polynesia) of the US GEOTRACES Pacific Meridional Transect (PMT) cruise (GP15, RR1815) on R/V Roger Revelle from Oct-Nov 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-11-13 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.914845.1 [access date]
GP15 HPLC pigments Leg 2 Dataset Description: Methods and Sampling: <p>Pigments were sampled from the shallowest 6 depths (&lt;=150 meters) on every "PigRaTh" cast (including the surface bottle) at all major stations (N=23 stations). Pigments were collected into 2-liter (L) amber bottles, triple-rinsed with sample prior to filling. They were immediately filtered under vacuum through 47-millimeter (mm) GF/F filters. They were folded and placed inside cryovials, labeled with appropriate GEOTRACES numbers, and frozen at -80° Celsius (C). Vials were shipped in LN2 dry shippers to Stanford, and then on dry ice to the HPLC Facility at Oregon State University (OSU) managed by Ricardo Letelier (<a href="https://ceoas.oregonstate.edu/ocean-ecology-and-biogeochemistry-facilities" target="_blank">https://ceoas.oregonstate.edu/ocean-ecology-and-biogeochemistry-facilities</a>).</p>
<p>OSU HPLC facility methods: Our method for pigment analysis is based on the protocol described by Mantoura and Lewellyn (1983) and modified according to Bidigare et al. (1989). Briefly, samples are extracted for up to 48 hours in 100% acetone at -20°C, then analyzed on a Waters 2690 separations module equipped with a C18 column and full spectrum photodiode array detector. Pigment standards for calibration and response factor calculations are obtained from DHI Group in Denmark.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1657781 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1657781
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1658318 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1658318
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1657944 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1657944
completed
Gregory A. Cutter
Old Dominion University
757-683-4929
Department of Ocean, Earth, and Atmospheric Sciences 4500 Elkhorn Ave
Norfolk
VA
23529-0276
USA
gcutter@odu.edu
pointOfContact
Karen L. Casciotti
Stanford University
650-721-5545
Department of Environmental Earth System Science 473 Via Ortega, Y2E2 Building, Room 140
Stanford
CA
94305
USA
kcasciotti@stanford.edu
pointOfContact
Phoebe J. Lam
University of California-Santa Cruz
831-459-4587
1156 High St. Department of Ocean Sciences
Santa Cruz
CA
95064
USA
pjlam@ucsc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Start_Date_UTC
Event_ID
Sample_ID
Niskin
Sample_Depth
Station_ID
Cast_number
Start_Latitude
Start_Longitude
But_fuco_HPLC_TP_CONC_BOTTLE_myzyux
Hex_fuco_HPLC_TP_CONC_BOTTLE_tlemch
Allo_HPLC_TP_CONC_BOTTLE_2aonag
Beta_Car_HPLC_TP_CONC_BOTTLE_ad2mxm
Alpha_Car_HPLC_TP_CONC_BOTTLE_rcv9le
Chl_a_HPLC_TP_CONC_BOTTLE_awnn1n
Chl_b_HPLC_TP_CONC_BOTTLE_ookljt
Chl_c1_chl_c2_HPLC_TP_CONC_BOTTLE_qboloy
Chl_c3_HPLC_TP_CONC_BOTTLE_h5hp8d
Chlide_a_HPLC_TP_CONC_BOTTLE_7imtld
Diadino_HPLC_TP_CONC_BOTTLE_o3xrsb
Diato_HPLC_TP_CONC_BOTTLE_uzdvgq
Fuco_HPLC_TP_CONC_BOTTLE_pd1qwn
Lut_HPLC_TP_CONC_BOTTLE_ligy3d
Neo_HPLC_TP_CONC_BOTTLE_zi32g3
Perid_HPLC_TP_CONC_BOTTLE_hawfd0
Pras_HPLC_TP_CONC_BOTTLE_8zmwaa
Viola_HPLC_TP_CONC_BOTTLE_35zxh0
Zea_HPLC_TP_CONC_BOTTLE_sw6srr
HPLC
theme
None, User defined
date
event
sample identification
bottle
depth
station
cast
latitude
longitude
19-prime-butanoyloxyfucoxanthin
19-prime-hexanoyloxyfucoxanthin
alloxanthin
carotene-beta
carotene-alpha
chlorophyll a
chlorophyll b
chl_c1_c2
chlorophyll c3
chlorophyllide
diadinoxanthin
diatoxanthin
fucoxanthin
lutein
neoxanthin
peridinin
prasinoxanthin
violaxanthin
zeaxanthin
featureType
BCO-DMO Standard Parameters
High-Performance Liquid Chromatograph
instrument
BCO-DMO Standard Instruments
RR1815
service
Deployment Activity
Hilo, HI to Papeete, French Polynesia
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
U.S. GEOTRACES
http://www.geotraces.org/
U.S. GEOTRACES
GEOTRACES is a SCOR sponsored program; and funding for program infrastructure development is provided by the U.S. National Science Foundation.
GEOTRACES gained momentum following a special symposium, S02: Biogeochemical cycling of trace elements and isotopes in the ocean and applications to constrain contemporary marine processes (GEOSECS II), at a 2003 Goldschmidt meeting convened in Japan. The GEOSECS II acronym referred to the Geochemical Ocean Section Studies To determine full water column distributions of selected trace elements and isotopes, including their concentration, chemical speciation, and physical form, along a sufficient number of sections in each ocean basin to establish the principal relationships between these distributions and with more traditional hydrographic parameters;
* To evaluate the sources, sinks, and internal cycling of these species and thereby characterize more completely the physical, chemical and biological processes regulating their distributions, and the sensitivity of these processes to global change; and
* To understand the processes that control the concentrations of geochemical species used for proxies of the past environment, both in the water column and in the substrates that reflect the water column.
GEOTRACES will be global in scope, consisting of ocean sections complemented by regional process studies. Sections and process studies will combine fieldwork, laboratory experiments and modelling. Beyond realizing the scientific objectives identified above, a natural outcome of this work will be to build a community of marine scientists who understand the processes regulating trace element cycles sufficiently well to exploit this knowledge reliably in future interdisciplinary studies.
Expand "Projects" below for information about and data resulting from individual US GEOTRACES research projects.
U.S. GEOTRACES
largerWorkCitation
program
US GEOTRACES Pacific Meridional Transect (GP15)
http://www.geotraces.org/
US GEOTRACES Pacific Meridional Transect (GP15)
<p>A 60-day research cruise took place in 2018 along a transect form Alaska to Tahiti at 152° W. A description of the project titled "<em>Collaborative Research: Management and implementation of the US GEOTRACES Pacific Meridional Transect</em>", funded by NSF, is below. Further project information is available on the <a href="https://www.geotraces.org/" target="_blank">US GEOTRACES website</a> and on the <a href="https://geotraces-gp15.com/" target="_blank">cruise blog</a>. A detailed <a href="https://datadocs.bco-dmo.org/docs/geotraces/GEOTRACES_PMT/casciotti/data_docs/GP15_Cruise_Report_with_ODF_Report.pdf" target="_blank">cruise report is also available</a> as a PDF.</p>
<p><em>Description from NSF award abstract:</em><br />
GEOTRACES is a global effort in the field of Chemical Oceanography in which the United States plays a major role. The goal of the GEOTRACES program is to understand the distributions of many elements and their isotopes in the ocean. Until quite recently, these elements could not be measured at a global scale. Understanding the distributions of these elements and isotopes will increase the understanding of processes that shape their distributions and also the processes that depend on these elements. For example, many "trace elements" (elements that are present in very low amounts) are also important for life, and their presence or absence can play a vital role in the population of marine ecosystems. This project will launch the next major U.S. GEOTRACES expedition in the Pacific Ocean between Alaska and Tahiti. The award made here would support all of the major infrastructure for this expedition, including the research vessel, the sampling equipment, and some of the core oceanographic measurements. This project will also support the personnel needed to lead the expedition and collect the samples.</p>
<p>This project would support the essential sampling operations and infrastructure for the U.S. GEOTRACES Pacific Meridional Transect along 152° W to support a large variety of individual science projects on trace element and isotope (TEI) biogeochemistry that will follow. Thus, the major objectives of this management proposal are: (1) plan and coordinate a 60 day research cruise in 2018; (2) obtain representative samples for a wide variety of TEIs using a conventional CTD/rosette, GEOTRACES Trace Element Sampling Systems, and in situ pumps; (3) acquire conventional CTD hydrographic data along with discrete samples for salinity, dissolved oxygen, algal pigments, and dissolved nutrients at micro- and nanomolar levels; (4) ensure that proper QA/QC protocols are followed and reported, as well as fulfilling all GEOTRACES intercalibration protocols; (5) prepare and deliver all hydrographic data to the GEOTRACES Data Assembly Centre (via the US BCO-DMO data center); and (6) coordinate all cruise communications between investigators, including preparation of a hydrographic report/publication. This project would also provide baseline measurements of TEIs in the Clarion-Clipperton fracture zone (~7.5°N-17°N, ~155°W-115°W) where large-scale deep sea mining is planned. Environmental impact assessments are underway in partnership with the mining industry, but the effect of mining activities on TEIs in the water column is one that could be uniquely assessed by the GEOTRACES community. In support of efforts to communicate the science to a wide audience the investigators will recruit an early career freelance science journalist with interests in marine science and oceanography to participate on the cruise and do public outreach, photography and/or videography, and social media from the ship, as well as to submit articles about the research to national media. The project would also support several graduate students.</p>
U.S. GEOTRACES PMT
largerWorkCitation
project
eng; USA
oceans
Hilo, HI to Papeete, French Polynesia
-152.015
-151.995
-19.9999
17.5
2018-10-27
2018-11-22
Pacific Meridional Transect along 152W (GP15)
0
BCO-DMO catalogue of parameters from Pigment concentrations determined by HPLC from samples collected on Leg 2 (Hilo, HI to Papeete, French Polynesia) of the US GEOTRACES Pacific Meridional Transect (PMT) cruise (GP15, RR1815) on R/V Roger Revelle from Oct-Nov 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/914860.rdf
Name: Start_Date_UTC
Units: unitless
Description: <p>Date (UTC) when sample was collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/914861.rdf
Name: Event_ID
Units: unitless
Description: <p>Event number</p>
http://lod.bco-dmo.org/id/dataset-parameter/914862.rdf
Name: Sample_ID
Units: unitless
Description: <p>GEOTRACES sample number</p>
http://lod.bco-dmo.org/id/dataset-parameter/914863.rdf
Name: Niskin
Units: unitless
Description: <p>Niskin bottle number</p>
http://lod.bco-dmo.org/id/dataset-parameter/914864.rdf
Name: Sample_Depth
Units: meters (m)
Description: <p>Sample depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/914865.rdf
Name: Station_ID
Units: unitless
Description: <p>Station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/914866.rdf
Name: Cast_number
Units: unitless
Description: <p>Cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/914867.rdf
Name: Start_Latitude
Units: decimal degrees
Description: <p>Latitude where sample was collected; positive values = North</p>
http://lod.bco-dmo.org/id/dataset-parameter/914868.rdf
Name: Start_Longitude
Units: decimal degrees
Description: <p>Longitude where samples were collected; negative values = West</p>
http://lod.bco-dmo.org/id/dataset-parameter/914869.rdf
Name: But_fuco_HPLC_TP_CONC_BOTTLE_myzyux
Units: nanograms per liter (ng/L)
Description: <p>Concentration of 19'-But-Fucoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/914870.rdf
Name: Hex_fuco_HPLC_TP_CONC_BOTTLE_tlemch
Units: nanograms per liter (ng/L)
Description: <p>Concentration of 19'-Hex-Fucoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/914871.rdf
Name: Allo_HPLC_TP_CONC_BOTTLE_2aonag
Units: nanograms per liter (ng/L)
Description: <p>Concentration of Alloxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/914872.rdf
Name: Beta_Car_HPLC_TP_CONC_BOTTLE_ad2mxm
Units: nanograms per liter (ng/L)
Description: <p>Concentration of Beta Carotene</p>
http://lod.bco-dmo.org/id/dataset-parameter/914873.rdf
Name: Alpha_Car_HPLC_TP_CONC_BOTTLE_rcv9le
Units: nanograms per liter (ng/L)
Description: <p>Concentration of Alpha Carotene</p>
http://lod.bco-dmo.org/id/dataset-parameter/914874.rdf
Name: Chl_a_HPLC_TP_CONC_BOTTLE_awnn1n
Units: nanograms per liter (ng/L)
Description: <p>Concentration of Chlorophyll a</p>
http://lod.bco-dmo.org/id/dataset-parameter/914875.rdf
Name: Chl_b_HPLC_TP_CONC_BOTTLE_ookljt
Units: nanograms per liter (ng/L)
Description: <p>Concentration of Chlorophyll b</p>
http://lod.bco-dmo.org/id/dataset-parameter/914876.rdf
Name: Chl_c1_chl_c2_HPLC_TP_CONC_BOTTLE_qboloy
Units: nanograms per liter (ng/L)
Description: <p>Concentration of Chlorophyll c 1&2</p>
http://lod.bco-dmo.org/id/dataset-parameter/914877.rdf
Name: Chl_c3_HPLC_TP_CONC_BOTTLE_h5hp8d
Units: nanograms per liter (ng/L)
Description: <p>Concentration of Chlorophyll c 3</p>
http://lod.bco-dmo.org/id/dataset-parameter/914878.rdf
Name: Chlide_a_HPLC_TP_CONC_BOTTLE_7imtld
Units: nanograms per liter (ng/L)
Description: <p>Concentration of Chlorophyllide</p>
http://lod.bco-dmo.org/id/dataset-parameter/914879.rdf
Name: Diadino_HPLC_TP_CONC_BOTTLE_o3xrsb
Units: nanograms per liter (ng/L)
Description: <p>Concentration of Diadinoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/914880.rdf
Name: Diato_HPLC_TP_CONC_BOTTLE_uzdvgq
Units: nanograms per liter (ng/L)
Description: <p>Concentration of Diatoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/914881.rdf
Name: Fuco_HPLC_TP_CONC_BOTTLE_pd1qwn
Units: nanograms per liter (ng/L)
Description: <p>Concentration of Fucoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/914882.rdf
Name: Lut_HPLC_TP_CONC_BOTTLE_ligy3d
Units: nanograms per liter (ng/L)
Description: <p>Concentration of Lutein</p>
http://lod.bco-dmo.org/id/dataset-parameter/914883.rdf
Name: Neo_HPLC_TP_CONC_BOTTLE_zi32g3
Units: nanograms per liter (ng/L)
Description: <p>Concentration of Neoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/914884.rdf
Name: Perid_HPLC_TP_CONC_BOTTLE_hawfd0
Units: nanograms per liter (ng/L)
Description: <p>Concentration of Peridinin</p>
http://lod.bco-dmo.org/id/dataset-parameter/914885.rdf
Name: Pras_HPLC_TP_CONC_BOTTLE_8zmwaa
Units: nanograms per liter (ng/L)
Description: <p>Concentration of Prasinoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/914886.rdf
Name: Viola_HPLC_TP_CONC_BOTTLE_35zxh0
Units: nanograms per liter (ng/L)
Description: <p>Concentration of Violaxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/914887.rdf
Name: Zea_HPLC_TP_CONC_BOTTLE_sw6srr
Units: nanograms per liter (ng/L)
Description: <p>Concentration of Zeaxanthin</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
9655
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/8be6fe71-addd-4dc2-9f2b-235ac51c11f4/content
download
https://doi.org/10.26008/1912/bco-dmo.914845.1
download
onLine
dataset
<p>Pigments were sampled from the shallowest 6 depths (&lt;=150 meters) on every "PigRaTh" cast (including the surface bottle) at all major stations (N=23 stations). Pigments were collected into 2-liter (L) amber bottles, triple-rinsed with sample prior to filling. They were immediately filtered under vacuum through 47-millimeter (mm) GF/F filters. They were folded and placed inside cryovials, labeled with appropriate GEOTRACES numbers, and frozen at -80° Celsius (C). Vials were shipped in LN2 dry shippers to Stanford, and then on dry ice to the HPLC Facility at Oregon State University (OSU) managed by Ricardo Letelier (<a href="https://ceoas.oregonstate.edu/ocean-ecology-and-biogeochemistry-facilities" target="_blank">https://ceoas.oregonstate.edu/ocean-ecology-and-biogeochemistry-facilities</a>).</p>
<p>OSU HPLC facility methods: Our method for pigment analysis is based on the protocol described by Mantoura and Lewellyn (1983) and modified according to Bidigare et al. (1989). Briefly, samples are extracted for up to 48 hours in 100% acetone at -20°C, then analyzed on a Waters 2690 separations module equipped with a C18 column and full spectrum photodiode array detector. Pigment standards for calibration and response factor calculations are obtained from DHI Group in Denmark.</p>
Specified by the Principal Investigator(s)
- Imported the second sheet ("HPLC_RR1816") of the original file "HPLC_Pigments_GP15_OSU_bco-dmo_barcodes.xlsx" into the BCO-DMO system.
- Renamed fields/columns to comply with BCO-DMO naming conventions.
- Converted Start_Date_UTC column to YYYY-mm-dd format.
- Saved the final file as "914845_v1_rr1815_hplc_pigments.csv".
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
HPLC
HPLC
PI Supplied Instrument Name: HPLC PI Supplied Instrument Description:Samples are analyzed on a Waters 2690 separations module equipped with a C18 column and full spectrum photodiode array detector. Samples are extracted and analyzed on a Waters 996 absorbance photodiode array detector in combination with a Waters 2475 fluorescence detector in September 2019 at OSU. Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/
Cruise: RR1815
RR1815
R/V Roger Revelle
Community Standard Description
International Council for the Exploration of the Sea
R/V Roger Revelle
vessel
RR1815
Gregory A. Cutter
Old Dominion University
https://datadocs.bco-dmo.org/docs/geotraces/GEOTRACES_PMT/casciotti/data_docs/GP15_Cruise_Report_with_ODF_Report.pdf
Report describing RR1815
R/V Roger Revelle
Community Standard Description
International Council for the Exploration of the Sea
R/V Roger Revelle
vessel