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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/916429.rdf" xlink:actuate="onRequest">Macronutrients and dissolved and particulate trace metals collected from the R/V Atlantic Explorer cruise AE1913 in the Sargasso Sea in June of 2019</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Saito, M. A., Cohen, N. (2024) Macronutrients and dissolved and particulate trace metals collected from the R/V Atlantic Explorer cruise AE1913 in the Sargasso Sea in June of 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-12-07 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.916429.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Dataset Description: &amp;lt;p&amp;gt;See the &amp;quot;Related Datasets&amp;quot; section on this page for the ELOG from cruise AE1913 and other closely related data.&amp;lt;br /&amp;gt;
*&amp;amp;nbsp;Also see all datasets related to the same cruise AE1913:&amp;amp;nbsp;https://www.bco-dmo.org/deployment/916412&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;Methods are described in a forthcoming manuscript by Cohen et al. Dissolved nitrate+nitrite, nitrite and silicate were measured on an Alpkem Rapid Flow Analyzer, and ammonium and phosphate were measured on a Technicon AutoAnalyzer II at Oregon State University.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For the dissolved metal analysis, filtered seawater was acidified to pH 1.8 using hydrochloric acid, and stored for 6 months at room temperature in the dark. Seawater preconcentration was performed using the seaFAST automated preconcentration system followed by quantification via inductively coupled plasma mass spectrometry. Reagents consisted of a 4M ammonium acetate pH 6.0 buffer prepared using high purity ammonium hydroxide and acetic acid, a 1% nitric acid rinse solution, 10% nitric acid elution acid, and a second internal standard 10% nitric elution acid solution containing 10 ppb indium (115In). Polypropylene conical tubes used with the autosampler were HCl acid-soaked and pH 2-rinsed prior to use. Process blanks consisted of Milli-Q HCl-acidified to pH 2 , and were run alongside samples. A stable isotope cocktail, which consisted of 57Fe, 61Ni, 65Cu, 67Zn and 111Cd, was spiked (50 µL) into each 15 mL sample to account for recovery and matrix effects. Following offline seaFAST preconcentration to 500 μL, the samples were analyzed using an iCAP Q inductively coupled plasma-mass spectrometer (ICP-MS). A six-point external standard curve was used with multi-element and indium (In) standards, diluted to range from 1-10 ppb in 5% nitric acid. Dissolved metal concentrations were determined using isotope dilution. Nitric acid (5%) injection blanks were subtracted from sample metal cps values except for Zn, in which injection blanks were generally higher than process blanks. Accuracy was determined using the 2009 Geotraces surface coastal (GSC) seawater intercalibration standard (n=5): dFe = 1.65 ± 0.19 nM [GSC = 1.56 ± 0.12 nM], dZn = 1.65 ± 0.17 nM [GSC = 1.45 ± 0.10 nM], dCu = 1.38 ± 0.16 nM [GSC = 1.12 ± 0.15 nM], dCd = 0.37 ± 0.03 nM [GSC = 0.37 ± 0.02 nM], dNi = 4.28 ± 0.17 nM [GSC = 4.5 ± 0.21 nM], and dMn = 2.42 ± 0.29 nM [GSC = 2.23 ± 0.08 nM]. The limit of detection was determined by calculating 3x the standard deviation of process blanks dataset-wide: dFe = 0.23 nM (n=14), dZn = 0.35 nM (n=16), dCu = 0.03 nM (n=18), dCd = 0.0103 nM (n=16), dNi = 0.07 nM (n=18), dMn = 0.03 nM (n=18). Blanks that were overtly contaminated with Fe (4 of 18), Zn (2 of 18) or Cd (2 of 18) were not included in the LOD estimation. In the case of Zn, high deep water (&amp;amp;gt;1,000 m) concentrations altered the spiked:stable isotope ratio, and accurate concentrations were not able to be obtained.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For the particulate metal analysis, whole 142 mm filters were extracted using an acid leachable digestion and quantified with ICP-MS. Process blanks were prepared in the laboratory and consisted of acid-cleaned Supor filters that were soaked for 1 week in oligotrophic seawater to condition with salt and remove residual metals introduced from high purity cleaning acids. Plastic forceps were used to place thawed filters into acid-cleaned polypropylene tubes containing 8mL of 5% nitric acid containing 1 ppb In. Filters were digested at 140°C for 3-4 hours, until less than ~4 mL was remaining. Filters were removed and the remainder of the solution was allowed to evaporate in the heat block until completely dry. Two mL of fresh 5% nitric acid was added to the precipitates. Tubes were vortexed, and 900 μL replicates of each sample were pipetted into an 81-well acid-rinsed plate alongside SPEX + In diluted standards. Metal quantification was performed using the iCAP Q ICP-MS. Particulate metal concentrations were calculated following Cox et al. 2014.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/765944.rdf" xlink:title="OCE-1658030" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1658030 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1658030</gmx:Anchor>
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	Name: Latitude
	Units: decimal degrees
	Description: &lt;p&gt;Latitude&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926581.rdf
	Name: Longitude
	Units: decimal degrees
	Description: &lt;p&gt;longitude&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926582.rdf
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	Units: meters (m)
	Description: &lt;p&gt;Depth of seawater collected using the trace metal clean rosette&lt;/p&gt; 
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http://lod.bco-dmo.org/id/dataset-parameter/926584.rdf
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	Units: unitless
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http://lod.bco-dmo.org/id/dataset-parameter/926585.rdf
	Name: dFe
	Units: nanomolar (nM)
	Description: &lt;p&gt;dissolved iron&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926586.rdf
	Name: dFeFlag
	Units: unitless
	Description: &lt;p&gt;quality control key value (see Data Processing section)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926587.rdf
	Name: dZn
	Units: nanomolar (nM)
	Description: &lt;p&gt;dissolved zinc&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926588.rdf
	Name: dZnFlag
	Units: unitless
	Description: &lt;p&gt;quality control key value (see Data Processing section)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926589.rdf
	Name: dCd
	Units: nanomolar (nM)
	Description: &lt;p&gt;dissolved cadmium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926590.rdf
	Name: dCdFlag
	Units: unitless
	Description: &lt;p&gt;quality control key value (see Data Processing section)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926591.rdf
	Name: dNi
	Units: nanomolar (nM)
	Description: &lt;p&gt;dissolved nickel&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926592.rdf
	Name: dNiFlag
	Units: unitless
	Description: &lt;p&gt;quality control key value (see Data Processing section)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926593.rdf
	Name: dCu
	Units: nanomolar (nM)
	Description: &lt;p&gt;dissolved copper&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926594.rdf
	Name: dCuFlag
	Units: unitless
	Description: &lt;p&gt;quality control key value (see Data Processing section)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926595.rdf
	Name: dMn
	Units: nanomolar (nM)
	Description: &lt;p&gt;dissolved manganese&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926596.rdf
	Name: dMnFlag
	Units: unitless
	Description: &lt;p&gt;quality control key value (see Data Processing section)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926597.rdf
	Name: PO4
	Units: micromolar (uM)
	Description: &lt;p&gt;dissolved phosphate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926598.rdf
	Name: NO2_NO3
	Units: micromolar (uM)
	Description: &lt;p&gt;dissolved nirate and nitrite&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926599.rdf
	Name: Si
	Units: micromolar (uM)
	Description: &lt;p&gt;dissolved silicate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926600.rdf
	Name: NO2
	Units: micromolar (uM)
	Description: &lt;p&gt;dissolved nitrite&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926601.rdf
	Name: NH4
	Units: micromolar (uM)
	Description: &lt;p&gt;dissolved ammonium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926602.rdf
	Name: pMn
	Units: picomolar (pM)
	Description: &lt;p&gt;particulate manganese&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926603.rdf
	Name: pFe
	Units: picomolar (pM)
	Description: &lt;p&gt;particulate iron&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926604.rdf
	Name: pNi
	Units: picomolar (pM)
	Description: &lt;p&gt;particulate nickel&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926605.rdf
	Name: pCu
	Units: picomolar (pM)
	Description: &lt;p&gt;particulate copper&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926606.rdf
	Name: pZn
	Units: picomolar (pM)
	Description: &lt;p&gt;particulate zinc&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/926607.rdf
	Name: pCd
	Units: picomolar (pM)
	Description: &lt;p&gt;particulate cadmium&lt;/p&gt; 
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&amp;lt;p&amp;gt;For the dissolved metal analysis, filtered seawater was acidified to pH 1.8 using hydrochloric acid, and stored for 6 months at room temperature in the dark. Seawater preconcentration was performed using the seaFAST automated preconcentration system followed by quantification via inductively coupled plasma mass spectrometry. Reagents consisted of a 4M ammonium acetate pH 6.0 buffer prepared using high purity ammonium hydroxide and acetic acid, a 1% nitric acid rinse solution, 10% nitric acid elution acid, and a second internal standard 10% nitric elution acid solution containing 10 ppb indium (115In). Polypropylene conical tubes used with the autosampler were HCl acid-soaked and pH 2-rinsed prior to use. Process blanks consisted of Milli-Q HCl-acidified to pH 2 , and were run alongside samples. A stable isotope cocktail, which consisted of 57Fe, 61Ni, 65Cu, 67Zn and 111Cd, was spiked (50 µL) into each 15 mL sample to account for recovery and matrix effects. Following offline seaFAST preconcentration to 500 μL, the samples were analyzed using an iCAP Q inductively coupled plasma-mass spectrometer (ICP-MS). A six-point external standard curve was used with multi-element and indium (In) standards, diluted to range from 1-10 ppb in 5% nitric acid. Dissolved metal concentrations were determined using isotope dilution. Nitric acid (5%) injection blanks were subtracted from sample metal cps values except for Zn, in which injection blanks were generally higher than process blanks. Accuracy was determined using the 2009 Geotraces surface coastal (GSC) seawater intercalibration standard (n=5): dFe = 1.65 ± 0.19 nM [GSC = 1.56 ± 0.12 nM], dZn = 1.65 ± 0.17 nM [GSC = 1.45 ± 0.10 nM], dCu = 1.38 ± 0.16 nM [GSC = 1.12 ± 0.15 nM], dCd = 0.37 ± 0.03 nM [GSC = 0.37 ± 0.02 nM], dNi = 4.28 ± 0.17 nM [GSC = 4.5 ± 0.21 nM], and dMn = 2.42 ± 0.29 nM [GSC = 2.23 ± 0.08 nM]. The limit of detection was determined by calculating 3x the standard deviation of process blanks dataset-wide: dFe = 0.23 nM (n=14), dZn = 0.35 nM (n=16), dCu = 0.03 nM (n=18), dCd = 0.0103 nM (n=16), dNi = 0.07 nM (n=18), dMn = 0.03 nM (n=18). Blanks that were overtly contaminated with Fe (4 of 18), Zn (2 of 18) or Cd (2 of 18) were not included in the LOD estimation. In the case of Zn, high deep water (&amp;amp;gt;1,000 m) concentrations altered the spiked:stable isotope ratio, and accurate concentrations were not able to be obtained.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For the particulate metal analysis, whole 142 mm filters were extracted using an acid leachable digestion and quantified with ICP-MS. Process blanks were prepared in the laboratory and consisted of acid-cleaned Supor filters that were soaked for 1 week in oligotrophic seawater to condition with salt and remove residual metals introduced from high purity cleaning acids. Plastic forceps were used to place thawed filters into acid-cleaned polypropylene tubes containing 8mL of 5% nitric acid containing 1 ppb In. Filters were digested at 140°C for 3-4 hours, until less than ~4 mL was remaining. Filters were removed and the remainder of the solution was allowed to evaporate in the heat block until completely dry. Two mL of fresh 5% nitric acid was added to the precipitates. Tubes were vortexed, and 900 μL replicates of each sample were pipetted into an 81-well acid-rinsed plate alongside SPEX + In diluted standards. Metal quantification was performed using the iCAP Q ICP-MS. Particulate metal concentrations were calculated following Cox et al. 2014.&amp;lt;/p&amp;gt;</gco:CharacterString>
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                <gco:CharacterString>&amp;lt;p&amp;gt;Dissolved metal concentrations below the limit of detection were removed from the final dataset.&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
For dissolved metals, flags were used following the Geotraces policy: https://www.geotraces.org/geotraces-quality-flag-policy/:&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;1 = good value&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;3 = probably bad value (for Zn, in samples with suspected 66Zn/67Zn (stable:spike) ratio too high)&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;4 = bad value (for Zn, in samples with suspected 66Zn/67Zn (stable:spike) ratio too high)&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;6 = below detection limit&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For macronutrients:&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;empty= no data value&amp;amp;nbsp; or removed due to oceanographically inconsistent profiles and potential thawing during sample transport.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Only select sites/depths were analyzed for particulate metals, the rest are blank.&amp;lt;/p&amp;gt;</gco:CharacterString>
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* File  &amp;quot;metals_bcodmo.csv&amp;quot; was imported into the BCO-DMO data system.
** Missing data values are displayed differently based on the file format you download.  They are blank in csv files, &amp;quot;NaN&amp;quot; in MatLab files, etc.
* * Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores.  Can not start with a number]
* Timestamps added to the data table for deployment and recovery times obtained from the ELOG GPS times for these events (see Related Datasets section for ELOG). The times in the elog are UTC time.  timestamp columns were added by joining on key &amp;quot;station&amp;quot; in metals_bcodmo.csv and second file supplied by the submitter stations_metals.csv which contained the station and timestamps.
* Format of timestamps converted to ISO 8601 format for timestamp with timeszone.</gco:CharacterString>
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