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            <gco:CharacterString>Cite this dataset as: Johnson, R. J., Bates, N. R., Lomas, M. W., Carlson, C. A., Smith, D., Lethaby, P. J., Lomas, D., Medley, C., May, R., Davey, E., Stuart, E., Garley, R., Derbyshire, L. (2026) Discrete bottle samples collected during BATS Validation (BVAL) cruises from April 1991 through July 2025. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 8) Version Date 2026-02-17 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.917255.8 [access date]</gco:CharacterString>
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        <gco:CharacterString>BATS Validation cruise bottle data Dataset Description: &amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;BATS Validation Cruises&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Following the first several years of the BATS project it was deemed necessary by the JGOFS steering committee and BATS PI’s to conduct validation cruises in the vicinity of the nominal BATS site to better understand the mesoscale and larger scale variability of the region. In particular, a focus of the BVAL cruises was to assess the spatial scale representation of the BATS and Hydrostation ‘S’ programs. Initial focus of the BVAL cruises was to investigate mesoscale variability and meridional gradients of the local region. Later, cruises focused on specific mesoscale eddies (e.g., Mcgillicuddy et al., 1998; McGillicuddy et al., 1999) and effects of tropical cyclones through the local region.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;In 2000 it was deemed more important to document the larger scale changes in the North Atlantic Subtropical gyre and BVAL cruises established a transect line from ~ 35N to 19N (Bermuda to Puerto Rico) very similar to the WOCE A22 repeat hydrography line (Johnson et al., 2020). These annual Bermuda to Puerto Rico transects have been run since 2000 and target stations at every one degree of latitude and typically have been conducted in September/October of each year to capture maximal heat content in the upper ocean. However, since this timeframe coincides with high tropical cyclone activity the cruises were reluctantly (as of 2022) moved to start in June/July of each year for safety and operational reasons. In the pentad prior to 2022 every BVAL cruise was significantly impacted but multiple tropical cyclones. Parameters presented are the same as provided in the BATS standard CTD data.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;&amp;lt;strong&amp;gt;Data Collection&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Data were collected on BVAL cruises from April 1991 (BVAL cruise #50001) through July 2025&amp;amp;nbsp;(BVAL cruise #50063).&amp;amp;nbsp;Research was conducted on many research vessels including R/V Weatherbird II, R/V Cape Henlopen, R/V Cape Hatteras and R/V Atlantic Explorer. Numerous Chief Scientists: Rachel Dow, Anthony Michaels, Kjell Gundersen, Rodney Johnson,&amp;amp;nbsp; Michael Lomas, Ann Close, Paul Lethaby, Vivienne Lochhead, Steven Bell, Gwyn Evans, Samuel Stevens, Claire Medley, and Dominic Smith.&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;&amp;lt;strong&amp;gt;Water sampling&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Full depth water sampling&amp;amp;nbsp;and data collection at the BATS site are achieved with a total of three hydrocasts using a General Oceanics Intelligent Rosette® with an array of 24 12L water bottles and a Sea-Bird Scientific CTD system. Water samples are collected during the upcast with a 1-minute resting period between reaching the sampling depth and triggering the bottle to close.&amp;amp;nbsp; Bottom measurements/ sampling are achieved within 20 meters from the bottom, as determined using an altimeter.&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;Water samples are taken right after Rosette® recovery. On any cast, if only a&amp;amp;nbsp;single water bottle is collected to sample all biogeochemical parameters, then gas samples are collected first due to their exposure to air when opened. However, if enough bottles are available, two bottles can be taken for a single depth. Water is usually split between large-volume particulate samples (POCN, HPLC, POP and PSi) and all other small volume samples, including gas samples. When two bottles are taken for a single depth, particulate samples are collected first to prevent settling within the Niskin bottle. Samples are fixed or frozen once all same-sample bottles from one cast have been collected. Particulate samples are filtered as soon as collected.&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;&amp;lt;strong&amp;gt;Nutrients&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
The BATS nutrient methodology is based on the Protocols for the &amp;lt;span style=&amp;quot;font-family:sans-serif,arial,verdana,trebuchet ms; font-size:14px&amp;quot;&amp;gt;Joint Global Ocean Flux Study (JGOFS) Core Measurements &amp;lt;/span&amp;gt;(&amp;lt;span style=&amp;quot;background-color:rgb(255, 255, 255); color:rgb(51, 51, 51); font-family:sans-serif,arial,verdana,trebuchet ms; font-size:14px&amp;quot;&amp;gt;Intergovernmental Oceanographic Commission, 1994) which&amp;amp;nbsp;&amp;lt;/span&amp;gt;describes the method for the determination of dissolved inorganic macronutrients in seawater: nitrite (NO2 – ), nitrate + nitrite (NO3 – + NO2 – ), orthophosphate (PO4 3 – ) and reactive silicate (Si(OH)4) using Continuous Flow Analysis (CFA).&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;While the definition of the dissolved fraction has changed throughout the years that the BATS time series has operated, the pore size used has remained constant in order to create a comparable temporal dataset. While similar studies in oligotrophic ocean regions have opted to forego the use of nutrient filters under the assumption that the particulate nutrient pool is negligible, we continue the use of filters for the sake of continuity. Sample filtration also removes the potential for turbidity-derived uncertainties during analysis, and may aid preservation of frozen samples.&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;Discrete samples are collected at the Bermuda Atlantic Time-series Study (BATS) site from surface to bottom depths (∼4,200 meters). Sea water is filtered directly from the Niskin spigot using a 0.8 µm membrane to remove particulates. Collected sea water is preserved by freezing until analysis. Replicate samples are taken during each cast to ensure quality control standards are met during analytical and data processes. Dissolved inorganic nutrients are measured using a SEAL AA500 Autoanalyzer by Continuous Flow Analysis (CFA). During this process, a subset of sample is drawn and further split into four different channels driven by a peristaltic pump. The sample stream is segmented with air or nitrogen bubbles throughout the flow path to enhance the mixing of reagents with the sample. The nutrients, NO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;-, nitrate + nitrite (NO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;– + NO2-) , PO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;3– &amp;lt;/sup&amp;gt;and Si,&amp;amp;nbsp;are chemically reacted in the separate channels to produce a color change and are&amp;amp;nbsp;measured colorimetrically at different wavelengths using a flow-through colorimeter located&amp;amp;nbsp;at the end of the flow path. The light absorption by the sample-reagent mixture is proportional to the concentration of nutrient in the sample according to the principles of the Beer-Lambert Law. Raw absorbance units are converted into nutrient concentrations according to a linear calibration curve formulated from known standards.&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;&amp;lt;strong&amp;gt;Bacterial enumeration&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
In addition to the casts for shallow water, mode water, and deep water, a separate cast is deployed for the estimation of bacterial growth rates using 3H-thymidine. Heterotrophic bacteria are expected to grow and assimilate 3H-thymidine into nucleic acid material under incubation conditions.&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;Three replicate samples from the same depth are used as live tubes for thymidine incorporation, and are incubated for four hours.&amp;amp;nbsp; Samples used as killed controls (aka kill tubes) are treated with 100 microliters of 100% TCA (trichloroacetic acid) at the beginning of the incubation to halt biological activity. After incubation, 10 microliters (µl) from the live tubes are extracted for Specific Activities measurements and the biological activity in the live tubes is halted by adding 100% TCA.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;All tubes are centrifuged at 14,000 RPM at 4°C for 7 minutes. The supernatant is discarded and DNA is extracted by adding 100% TCA; centrifuging again for 7 minutes at 4°C at 14,000 RPM;&amp;amp;nbsp; adding 80% ethanol and centrifuging once more for 7 minutes at 4°C at 14,000 RPM. The DNA in the resulting pellet is resuspended in Ultima Gold by vortexing. Samples are stored at room temperature until analysis.&amp;lt;/p&amp;gt;

&amp;lt;div&amp;gt;
&amp;lt;div&amp;gt;
&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;&amp;lt;strong&amp;gt;Full methodology&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Detailed methods are available&amp;amp;nbsp;in Knap et al. (1997).&amp;lt;/p&amp;gt;
&amp;lt;/div&amp;gt;
&amp;lt;/div&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;&amp;amp;nbsp;&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/936790.rdf" xlink:title="OCE-2241455" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2241455 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2241455</gmx:Anchor>
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Decades of research have demonstrated that the ocean varies across a range of time scales, with anthropogenic forcing contributing an added layer of complexity. In a growing effort to distinguish between natural and human-induced earth system variability, sustained ocean time-series measurements have taken on a renewed importance. Shipboard biogeochemical time-series represent one of the most valuable tools scientists have to characterize and quantify ocean carbon fluxes and biogeochemical processes and their links to changing climate (Karl, 2010; Chavez et al., 2011; Church et al., 2013). They provide the oceanographic community with the long, temporally resolved datasets needed to characterize ocean climate, biogeochemistry, and ecosystem change.
The temporal scale of shifts in marine ecosystem variations in response to climate change are on the order of several decades.  The long-term, consistent and comprehensive monitoring programs conducted by time-series sites are essential to understand large-scale atmosphere-ocean interactions that occur on interannual to decadal time scales.  Ocean time-series represent one of the most valuable tools scientists have to characterize and quantify ocean carbon fluxes and biogeochemical processes and their links to changing climate.
Launched in the late 1980s, the US JGOFS (Joint Global Ocean Flux Study; http://usjgofs.whoi.edu) research program initiated two time-series measurement programs at Hawaii and Bermuda (HOT and BATS, respectively) to measure key oceanographic measurements in oligotrophic waters. Begun in 1995 as part of the US JGOFS Synthesis and Modeling Project, the CARIACO Ocean Time-Series (formerly known as the CArbon Retention In A Colored Ocean) Program has studied the relationship between surface primary production, physical forcing variables like the wind, and the settling flux of particulate carbon in the Cariaco Basin.
The objective of these time-series effort is to provide well-sampled seasonal resolution of biogeochemical variability at a limited number of ocean observatories, provide support and background measurements for process-oriented research, as well as test and validate observations for biogeochemical models. Since their creation, the BATS, CARIACO and HOT time-series site data have been available for use by a large community of researchers.
 
Data from those three US funded, ship-based, time-series sites can be accessed at each site directly or by selecting the site name from the Projects section below.
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                            <gco:CharacterString>&lt;p&gt;A full description of the BATS research program (including links to the processed BATS data) is available from the BATS Web site (see above for Project URL/ Project Website links). Any data contributed from selected ancillary projects are listed (linked) in the 'Datasets Collection' section below.  &lt;/p&gt;
&lt;p&gt;&lt;strong&gt;Collaborative Research: The Bermuda Atlantic Time-series Study: Sustained Biogeochemical, Ecosystem and Ocean Change Observations and Linkages in the North Atlantic (Years 36-40)&lt;/strong&gt;&lt;br /&gt;
&lt;em&gt;&lt;strong&gt;Awards OCE-&lt;/strong&gt;&lt;/em&gt;&lt;strong&gt;2241455&lt;em&gt;, OCE-&lt;/em&gt;2241456&lt;em&gt; and OCE-&lt;/em&gt;2241457&lt;em&gt;)&lt;/em&gt;&lt;/strong&gt;&lt;br /&gt;
&lt;u&gt;NSF award abstract&lt;/u&gt;&lt;/p&gt;
&lt;p&gt;Long-term observations of ocean physics, biology, and chemistry across decades provide a powerful lens for understanding the response of the oceans to environmental change. This award will continue the Bermuda Atlantic Time-series Study (BATS) research program, which began in 1988, for another five years. Observations at the BATS site provide crucial information for understanding the ocean?s role in the global climate system and the response of the ocean carbon system and marine ecosystems to climate perturbations. The research goals of the BATS program continue to be to improve our understanding of the time-varying components of the ocean carbon cycle and related elements of interest (such as nitrogen, phosphorus, and silica) and to identify the physical, chemical, and ecosystem properties responsible for this variability. The BATS program has substantial broader impacts, contributing to the field of ocean sciences by providing high-quality ocean observations and a framework in which other researchers can conceive and test hypotheses. In addition, the recent acquisition of the Bermuda Institute of Ocean Sciences by the Global Futures Laboratory of Arizona State University provides new avenues for educational opportunities and innovation.&lt;/p&gt;
&lt;p&gt;In the subtropical gyre of the North Atlantic Ocean, warming, salinification, deoxygenation, ocean ecosystem changes, and acidification have accelerated their rate of change. Fundamental questions and challenges remain about understanding present and future ocean function, prediction, and modelling. An overarching question for the BATS program is: Will ocean biogeochemistry and ecosystem functioning continue to change in response to the acceleration of ocean warming, salinification, stratification, deoxygenation and acidification? With this question in mind, the sustained goals for the BATS program are: 1. Quantify the role of ocean-atmosphere coupling and climate variability on air-sea exchange of carbon dioxide (CO2) and carbon export to the ocean interior; 2. Document trends and controls of the following: (a) the interannual to decadal scale variability in carbon and nutrient cycles and their coupling in the surface and deep ocean via the Redfield Ratio paradigm; and, (b) biological community structure in the oligotrophic North Atlantic Ocean in response to low-frequency climate variability; 3. Quantify the response of planktonic and microbial community structure and function and impact on biogeochemical cycles (including new and export productivity) to variability in surface fluxes (e.g., heat, freshwater and momentum) and physical processes (e.g., mesoscale eddies, Rossby Waves, internal waves); 4. Facilitate development, calibration and validation of next-generation oceanographic sensors, tools and technologies; 5. Generate datasets that can be used by empiricists and modelers to test new hypotheses about North Atlantic Ocean biogeochemistry and ecosystem functioning; 6. Use BATS cruise, infrastructure, laboratory and analytical expertise, and data to improve education and training programs for BATS staff, STEM-literate students, and future oceanographers.&lt;/p&gt;
&lt;p&gt;This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.&lt;/p&gt;
&lt;p&gt;Please see the BATS Web site (&lt;a href=&quot;http://bats.bios.edu&quot; target=&quot;_blank&quot;&gt;http://bats.bios.edu&lt;/a&gt;) for additional information.&lt;/p&gt;
&lt;p&gt;&lt;a href=&quot;http://data.bco-dmo.org/BATS/BATS_References.pdf&quot;&gt;List of References (PDF)&lt;/a&gt;&lt;/p&gt;</gco:CharacterString>
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	Name: Bottle_ID
	Units: unitless
	Description: &lt;p&gt;A unique bottle ID which identifies cruise, cast, and Niskin number&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947508.rdf
	Name: ISO_DateTime_UTC
	Units: unitless
	Description: &lt;p&gt;Sampling date in ISO8601 format&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947509.rdf
	Name: Vessel
	Units: unitless
	Description: &lt;p&gt;Name of vessel used for cruise&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947510.rdf
	Name: Latitude
	Units: decimal degrees
	Description: &lt;p&gt;Latitude of sampling&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947511.rdf
	Name: Longitude
	Units: decimal degrees
	Description: &lt;p&gt;Longitude of sampling&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947512.rdf
	Name: Cruise_type
	Units: unitless
	Description: &lt;p&gt;Cruise type (BATS Validation)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947513.rdf
	Name: Cruise_num
	Units: unitless
	Description: &lt;p&gt;Cruise number where the 5 represents a BATS Validation cruise followed by the BATS cruise ID&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947515.rdf
	Name: Cast_num
	Units: unitless
	Description: &lt;p&gt;Cast number where 1-80 are CTD casts and 81-99 are Hydrocasts&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947516.rdf
	Name: Bottle_num
	Units: unitless
	Description: &lt;p&gt;Niskin (or GoFlo) bottle number&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947517.rdf
	Name: QF_Bottle
	Units: unitless
	Description: &lt;p&gt;Quality flag for bottle (-3 = suspect, 1 = unverified, 2 = verified/acceptable)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947518.rdf
	Name: Depth
	Units: meters (m)
	Description: &lt;p&gt;Depth of sampling&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947519.rdf
	Name: QF_Depth
	Units: unitless
	Description: &lt;p&gt;Quality flag for depth; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947520.rdf
	Name: Temp
	Units: degrees Celsius
	Description: &lt;p&gt;Temperature (ITS-90 scale)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947521.rdf
	Name: QF_Temp
	Units: unitless
	Description: &lt;p&gt;Quality flag for temperature; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947522.rdf
	Name: CTD_Sal
	Units: PSS-78
	Description: &lt;p&gt;CTD Salinity (PSS-78 scale)      &lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947523.rdf
	Name: QF_CTD_Sal
	Units: unitless
	Description: &lt;p&gt;Quality flag for CTD salinity; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947524.rdf
	Name: Sal1
	Units: PSS-78
	Description: &lt;p&gt;Salinity-1 (PSS-78 scale)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947525.rdf
	Name: QF_Sal1
	Units: unitless
	Description: &lt;p&gt;Quality flag for Salinity-1; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947526.rdf
	Name: Sigma_theta
	Units: kilogram per cubic meter (kg/m^3)
	Description: &lt;p&gt;Sigma-theta potential density&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947527.rdf
	Name: QF_Sigma_theta
	Units: unitless
	Description: &lt;p&gt;Quality flag for sigma-theta; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947528.rdf
	Name: O2
	Units: micromole per kilogram (umol/kg)
	Description: &lt;p&gt;Oxygen-1&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947529.rdf
	Name: QF_O2
	Units: unitless
	Description: &lt;p&gt;Quality flag for oxygen; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947530.rdf
	Name: DIC
	Units: micromole per kilogram (umol/kg)
	Description: &lt;p&gt;Dissolved inorganic carbon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947531.rdf
	Name: QF_DIC
	Units: unitless
	Description: &lt;p&gt;Quality flag for DIC (dissolved inorganic carbon); Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947532.rdf
	Name: Alkalinity
	Units: microequivalents (uequiv)
	Description: &lt;p&gt;Alkalinity&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947533.rdf
	Name: QF_Alkalinity
	Units: unitless
	Description: &lt;p&gt;Quality flag for alkalinity; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947534.rdf
	Name: NO3_NO2
	Units: micromole per kilogram (umol/kg)
	Description: &lt;p&gt;Nitrate + Nitrite&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947535.rdf
	Name: QF_NO3_NO2
	Units: unitless
	Description: &lt;p&gt;Quality flag for nitrate + nitrite; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947536.rdf
	Name: NO2
	Units: micromole per kilogram (umol/kg)
	Description: &lt;p&gt;Nitrite&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947537.rdf
	Name: QF_NO2
	Units: unitless
	Description: &lt;p&gt;Quality flag for nitrite; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947538.rdf
	Name: PO4
	Units: micromole per kilogram (umol/kg)
	Description: &lt;p&gt;Phosphate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947539.rdf
	Name: QF_PO4
	Units: unitless
	Description: &lt;p&gt;Quality flag for phosphate; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947540.rdf
	Name: Silicate
	Units: micromole per kilogram (umol/kg)
	Description: &lt;p&gt;Silicate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947541.rdf
	Name: QF_Silicate
	Units: unitless
	Description: &lt;p&gt;Quality flag for silicate; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947542.rdf
	Name: POC
	Units: micrograms per kilogram (ug/kg)
	Description: &lt;p&gt;Particulate organic carbon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947543.rdf
	Name: QF_POC
	Units: unitless
	Description: &lt;p&gt;Quality flag for POC; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947544.rdf
	Name: PON
	Units: micrograms per kilogram (ug/kg)
	Description: &lt;p&gt;Particulate organic nitrogen&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947545.rdf
	Name: QF_PON
	Units: unitless
	Description: &lt;p&gt;Quality flag for PON; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947546.rdf
	Name: TOC
	Units: micromole per kilogram (umol/kg)
	Description: &lt;p&gt;Total organic carbon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947547.rdf
	Name: QF_TOC
	Units: unitless
	Description: &lt;p&gt;Quality flag for TOC; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947548.rdf
	Name: TN
	Units: micromole per kilogram (umol/kg)
	Description: &lt;p&gt;Total nitrogen&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947549.rdf
	Name: QF_TN
	Units: unitless
	Description: &lt;p&gt;Quality flag for total nitrogen; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947550.rdf
	Name: Bact
	Units: cells times 10^8 per kilogram (cells*10^8/kg)
	Description: &lt;p&gt;Bacteria enumeration&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947551.rdf
	Name: QF_Bact
	Units: unitless
	Description: &lt;p&gt;Quality flag for bacteria enumeration; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947552.rdf
	Name: POP
	Units: micromole per kilogram (umol/kg)
	Description: &lt;p&gt;Particulate organic phosphorus&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947553.rdf
	Name: QF_POP
	Units: unitless
	Description: &lt;p&gt;Quality flag for POP; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947554.rdf
	Name: TDP
	Units: nanomole per kilogram (nmol/kg)
	Description: &lt;p&gt;Total dissolved phosphorus&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947555.rdf
	Name: QF_TDP
	Units: unitless
	Description: &lt;p&gt;Quality flag for TDP; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947556.rdf
	Name: SRP
	Units: nanomole per kilogram (nmol/kg)
	Description: &lt;p&gt;Low-level phosphorus&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947557.rdf
	Name: QF_SRP
	Units: unitless
	Description: &lt;p&gt;Quality flag for low-level phosphorus; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947558.rdf
	Name: Bio_Si
	Units: micromole per kilogram (umol/kg)
	Description: &lt;p&gt;Particulate biogenic silica&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947559.rdf
	Name: QF_Bio_Si
	Units: unitless
	Description: &lt;p&gt;Quality flag for particulate biogenic silica; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947560.rdf
	Name: Lith_Si
	Units: micromole per kilogram (umol/kg)
	Description: &lt;p&gt;Particulate lithogenic silica&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947561.rdf
	Name: QF_Lith_Si
	Units: unitless
	Description: &lt;p&gt;Quality flag for particulate lithogenic silica; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947562.rdf
	Name: Prochlorococcus
	Units: cells per milliliter (cells/mL)
	Description: &lt;p&gt;Prochlorococcus abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947563.rdf
	Name: QF_Prochlorococcus
	Units: unitless
	Description: &lt;p&gt;Quality flag for prochlorococcus abundance; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947564.rdf
	Name: Synechococcus
	Units: cells per milliliter (cells/mL)
	Description: &lt;p&gt;Synechococcus abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947565.rdf
	Name: QF_Synechococcus
	Units: unitless
	Description: &lt;p&gt;Quality flag for synechococcus abundance; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947566.rdf
	Name: Picoeukaryotes
	Units: cells per milliliter (cells/mL)
	Description: &lt;p&gt;Picoeukaryote abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947567.rdf
	Name: QF_Picoeukaryotes
	Units: unitless
	Description: &lt;p&gt;Quality flag for picoeukaryote abundance; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947568.rdf
	Name: Nanoeukaryotes
	Units: cells per milliliter (cells/mL)
	Description: &lt;p&gt;Nanoeukaryote abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947569.rdf
	Name: QF_Nanoeukaryotes
	Units: unitless
	Description: &lt;p&gt;Quality flag for nanoeukaryote abundance; Parameter quality flags defined as 1= unverified, 2= verified acceptable, 3= questionable, 4= bad, 9= no data&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947570.rdf
	Name: decy
	Units: unitless
	Description: &lt;p&gt;Decimal Year&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947571.rdf
	Name: time
	Units: unitless
	Description: &lt;p&gt;Time in Hour Minute format (hhmm)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/947572.rdf
	Name: yyyymmdd
	Units: unitless
	Description: &lt;p&gt;Date in Year Month Day format&lt;/p&gt; 
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                <gco:CharacterString>&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;&amp;lt;strong&amp;gt;Data Collection&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Data were collected on BVAL cruises from April 1991 (BVAL cruise #50001) through July 2025&amp;amp;nbsp;(BVAL cruise #50063).&amp;amp;nbsp;Research was conducted on many research vessels including R/V Weatherbird II, R/V Cape Henlopen, R/V Cape Hatteras and R/V Atlantic Explorer. Numerous Chief Scientists: Rachel Dow, Anthony Michaels, Kjell Gundersen, Rodney Johnson,&amp;amp;nbsp; Michael Lomas, Ann Close, Paul Lethaby, Vivienne Lochhead, Steven Bell, Gwyn Evans, Samuel Stevens, Claire Medley, and Dominic Smith.&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;&amp;lt;strong&amp;gt;Water sampling&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Full depth water sampling&amp;amp;nbsp;and data collection at the BATS site are achieved with a total of three hydrocasts using a General Oceanics Intelligent Rosette® with an array of 24 12L water bottles and a Sea-Bird Scientific CTD system. Water samples are collected during the upcast with a 1-minute resting period between reaching the sampling depth and triggering the bottle to close.&amp;amp;nbsp; Bottom measurements/ sampling are achieved within 20 meters from the bottom, as determined using an altimeter.&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;Water samples are taken right after Rosette® recovery. On any cast, if only a&amp;amp;nbsp;single water bottle is collected to sample all biogeochemical parameters, then gas samples are collected first due to their exposure to air when opened. However, if enough bottles are available, two bottles can be taken for a single depth. Water is usually split between large-volume particulate samples (POCN, HPLC, POP and PSi) and all other small volume samples, including gas samples. When two bottles are taken for a single depth, particulate samples are collected first to prevent settling within the Niskin bottle. Samples are fixed or frozen once all same-sample bottles from one cast have been collected. Particulate samples are filtered as soon as collected.&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;&amp;lt;strong&amp;gt;Nutrients&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
The BATS nutrient methodology is based on the Protocols for the &amp;lt;span style=&amp;quot;font-family:sans-serif,arial,verdana,trebuchet ms; font-size:14px&amp;quot;&amp;gt;Joint Global Ocean Flux Study (JGOFS) Core Measurements &amp;lt;/span&amp;gt;(&amp;lt;span style=&amp;quot;background-color:rgb(255, 255, 255); color:rgb(51, 51, 51); font-family:sans-serif,arial,verdana,trebuchet ms; font-size:14px&amp;quot;&amp;gt;Intergovernmental Oceanographic Commission, 1994) which&amp;amp;nbsp;&amp;lt;/span&amp;gt;describes the method for the determination of dissolved inorganic macronutrients in seawater: nitrite (NO2 – ), nitrate + nitrite (NO3 – + NO2 – ), orthophosphate (PO4 3 – ) and reactive silicate (Si(OH)4) using Continuous Flow Analysis (CFA).&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;While the definition of the dissolved fraction has changed throughout the years that the BATS time series has operated, the pore size used has remained constant in order to create a comparable temporal dataset. While similar studies in oligotrophic ocean regions have opted to forego the use of nutrient filters under the assumption that the particulate nutrient pool is negligible, we continue the use of filters for the sake of continuity. Sample filtration also removes the potential for turbidity-derived uncertainties during analysis, and may aid preservation of frozen samples.&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;Discrete samples are collected at the Bermuda Atlantic Time-series Study (BATS) site from surface to bottom depths (∼4,200 meters). Sea water is filtered directly from the Niskin spigot using a 0.8 µm membrane to remove particulates. Collected sea water is preserved by freezing until analysis. Replicate samples are taken during each cast to ensure quality control standards are met during analytical and data processes. Dissolved inorganic nutrients are measured using a SEAL AA500 Autoanalyzer by Continuous Flow Analysis (CFA). During this process, a subset of sample is drawn and further split into four different channels driven by a peristaltic pump. The sample stream is segmented with air or nitrogen bubbles throughout the flow path to enhance the mixing of reagents with the sample. The nutrients, NO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;-, nitrate + nitrite (NO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;– + NO2-) , PO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;3– &amp;lt;/sup&amp;gt;and Si,&amp;amp;nbsp;are chemically reacted in the separate channels to produce a color change and are&amp;amp;nbsp;measured colorimetrically at different wavelengths using a flow-through colorimeter located&amp;amp;nbsp;at the end of the flow path. The light absorption by the sample-reagent mixture is proportional to the concentration of nutrient in the sample according to the principles of the Beer-Lambert Law. Raw absorbance units are converted into nutrient concentrations according to a linear calibration curve formulated from known standards.&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;&amp;lt;strong&amp;gt;Bacterial enumeration&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
In addition to the casts for shallow water, mode water, and deep water, a separate cast is deployed for the estimation of bacterial growth rates using 3H-thymidine. Heterotrophic bacteria are expected to grow and assimilate 3H-thymidine into nucleic acid material under incubation conditions.&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;Three replicate samples from the same depth are used as live tubes for thymidine incorporation, and are incubated for four hours.&amp;amp;nbsp; Samples used as killed controls (aka kill tubes) are treated with 100 microliters of 100% TCA (trichloroacetic acid) at the beginning of the incubation to halt biological activity. After incubation, 10 microliters (µl) from the live tubes are extracted for Specific Activities measurements and the biological activity in the live tubes is halted by adding 100% TCA.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;All tubes are centrifuged at 14,000 RPM at 4°C for 7 minutes. The supernatant is discarded and DNA is extracted by adding 100% TCA; centrifuging again for 7 minutes at 4°C at 14,000 RPM;&amp;amp;nbsp; adding 80% ethanol and centrifuging once more for 7 minutes at 4°C at 14,000 RPM. The DNA in the resulting pellet is resuspended in Ultima Gold by vortexing. Samples are stored at room temperature until analysis.&amp;lt;/p&amp;gt;

&amp;lt;div&amp;gt;
&amp;lt;div&amp;gt;
&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;&amp;lt;strong&amp;gt;Full methodology&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Detailed methods are available&amp;amp;nbsp;in Knap et al. (1997).&amp;lt;/p&amp;gt;
&amp;lt;/div&amp;gt;
&amp;lt;/div&amp;gt;

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- Modified parameter names to conform with BCO-DMO naming conventions and to be more consistent with other BATS data submissions

** Version 7 **
There are no new cruises for this version. This update includes new values for measurements made since the last version (bacterial abundance, FCM, etc.) See release notes supplemental file for more information
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