http://lod.bco-dmo.org/id/dataset/917767 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2024-01-02 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) In situ tracer injection experiment conducted with 15N-labeled ammonium in a shallow, sandy subterranean estuary in Gloucester Point, USA in August 2019. 2024-01-15 publication 2024-01-15 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2024-01-15 publication https://doi.org/10.26008/1912/bco-dmo.917767.1 Bongkeun Song Virginia Institute of Marine Science principalInvestigator Iris C. Anderson Virginia Institute of Marine Science principalInvestigator Craig Tobias University of Connecticut principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Wilson, S. J., Song, B., Tobias, C., Anderson, I. C. (2024) In situ tracer injection experiment conducted with 15N-labeled ammonium in a shallow, sandy subterranean estuary in Gloucester Point, USA in August 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-01-15 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.917767.1 [access date] Methods and Sampling: &lt;p&gt;These data were collected during August 2019 from a sandy subterranean estuary (STE) located in Gloucester Point, Virginia, USA. Porewater was collected from surface water and piezometers placed at 40, 50, and 60cm. Prior to the injection, porewater was collected from replicate 50cm piezometers and amended with 15N-labeled ammonium, sulfur hexaflouride, and bromide. The amended porewater was then reintroduced into the STE through the 50cm injection piezometers.&lt;/p&gt; &lt;p&gt;After injection porewater was collected overtime from both the injection piezometers and other piezometers surrounding the injection well. At each time point, porewater was collected in serum bottles prepared with potassium hydroxide and were equlibrated by shaking before being run on a gas chromatograph with electron capture detection (GC-ECD, Shimadzu) in order to determine the sulfur hexaflouride concentration. Porewater was also collected for inorganic nutrients and ions, filtered with a 0.45 µm syringe filter (Whatman GE) and were frozen (-20 °C) until analysis.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1657801 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1657801 completed Bongkeun Song Virginia Institute of Marine Science 804-684-7411 P.O Box 1346 Gloucester Point VA 23692 USA songb@vims.edu pointOfContact Iris C. Anderson Virginia Institute of Marine Science 757-876-9436 P.O Box 1346 Gloucester Point VA 23062 USA iris@vims.edu pointOfContact Craig Tobias University of Connecticut 860-405-9140 1080 Shennecossett Rd. Groton CT 06340 USA craig.tobias@uconn.edu pointOfContact asNeeded Dataset Version: 1 Unknown Site_Name Latitude Longitude Piezometer Sample_ID Time_Point Date Time Hours_after_Injection Bromide_uM Chloride_mM SF6_ppbv SF6_pM Nox_uM NO2_uM NH4_uM True_d15N Mole_Fraction_Calc Ion Chromatograph, ThermoFisher Scientific; Lachat QuikChem 8000 automated ion analyzer Lachat In- struments theme None, User defined site latitude longitude sample description sample identification time_point date time of day incubation time or duration bromides chloride SF6 nitrate plus nitrite Nitrite Ammonium d15N featureType BCO-DMO Standard Parameters Lachat QuikChem 8000 flow injection analyzer and Ion Chromatography (IC) system instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: Cryptic nitrogen cycling in the anoxic subterranean estuary https://osprey.bco-dmo.org/project/805722 Collaborative Research: Cryptic nitrogen cycling in the anoxic subterranean estuary <p><em>NSF Award Abstract:</em><br /> Nitrogen is an important nutrient that maintains high coastal ecosystem productivity. Yet excess nitrogen delivery can cause serious water quality deterioration including harmful algal blooms, fish kills, and oxygen free dead zones. Numerous nitrogen transformations regulate the balance between nitrogen delivery and nitrogen removal in coastal environments and the majority of these reactions occur in sediments where seawater passes through the subsurface and mixes with groundwater transported from uplands. This mixing zone, referred to as the subterranean estuary, is characterized by very different geochemistry than either the seawater above it or the groundwater below it. Thus, it has the potential to host a variety of unique reactions that affect nitrogen availability to the overlying water. Scientists from the College of William and Mary, Virginia Institute of Marine Science (VIMS), and the University of Connecticut (UConn) propose to examine the importance of a cryptic nitrogen cycle, a novel and potentially widespread nitrogen cycling process in the subterranean estuary. The cryptic nitrogen cycle comprises anoxic ammonium oxidation to nitrite (anoxic nitrification) coupled with anaerobic ammonium oxidation (anammox) or denitrification producing harmless dinitrogen gas. The proposed project represents highly transformative science because it has the potential to change the current paradigm detailing operation of the biogeochemical nitrogen cycle in anoxic environments. Occurrence of the cryptic nitrogen cycle would have broad implications for the nitrogen budget of terrestrial and groundwater systems and the coastal ocean. Characterization of the cryptic nitrogen cycle will allow us to better understand interactions among the nitrogen, metals, and sulfur cycles, and potential impacts of ongoing human modification of coastal environments. Educational contribution of this project focuses on graduate and undergraduate student training. Two graduate students at VIMS and UConn will receive interdisciplinary training in microbiology, molecular ecology, and biogeochemistry while several undergraduates recruited through the VIMS REU (Research Experience for Undergraduates) Program and the UConn marine science programs will also participate in the project. In addition, three summer undergraduate interns will be recruited from Hampton University, a historically Black college, and trained to enhance minority education and research in marine science. Public outreach will be achieved through popular venues such as VIMS Marine Science Day, and the VIMS After Hours Public Lecture Series at VIMS. Tobias at UConn also provides educational contributions and outreach efforts through the UConn Marine Scholars and Early College Experience programs and an exhibit at Mystic Aquarium.</p> <p>A cryptic nitrogen cycle is proposed as a new process coupling anoxic nitrification to microbial nitrogen removal pathways such as anammox and denitrification. Unlike anammox, which refers to the oxidation of ammonium by nitrite to form dinitrogen (N2) gas, anoxic nitrification occurs by oxidation of ammonium in the absence of oxygen using other common chemical oxidants such as metal oxides (namely, Fe and Mn) or sulfate, abundant in many marine and coastal systems. The thermodynamic favorability of these reactions relies on coupling nitrite formed via these oxidants with anammox or denitrification. Due to the coupling, nitrite will not accumulate or be measurable in anoxic marine systems. Thus, a cryptic N cycle responsible for nitrite production can occur as a novel N transforming process in anoxic environments, serve as a vital link to N2 production, and attenuate N loads discharging from a subterranean estuary (STE). Preliminary results from a STE in the York River Estuary located in Virginia showed substantial N2 production, representing removal of 50-75% of the fixed groundwater N, in ferruginous and sulfidic zones where neither nitrite nor nitrate were detectable. Stable isotope incubation experiments using the 15N tracer and molecular analysis of microbial communities suggest that coupled anoxic nitrification and anammox processes are the dominant N2 production pathways rather than canonical denitrification in the STE. Therefore, coupled anoxic nitrification-anammox in coastal groundwater may be a major unrecognized sink for fixed nitrogen at the land-sea interface. In addition to coastal groundwater, the cryptic N cycle has potential importance in anoxic zones and ocean basins. This proposal focuses on the STE because geochemical conditions there appear optimal for the proposed reactions to occur, and our preliminary data show strong evidence for a cryptic N cycle. The proposed work uses a combined geochemical, 15N isotope tracer and microbiological approach to evaluate environmental controls on the cryptic N cycle as well as to estimate its contribution to reduction of fixed N fluxes to the coastal ocean. Four approaches are proposed: (1) Field characterization of anoxic nitrification reactions and associated microbial communities in a subterranean estuary; (2) Laboratory incubation experiments to identify hotspots of the cryptic N cycle; (3) Controlled microcosm experiments to determine geochemical controls on anoxic nitrification; and (4) in situ assessment of anoxic nitrification to estimate the importance of the cryptic N cycle in a coastal aquifer.</p> Subsurface cryptic N cycle largerWorkCitation project eng; USA oceans -76.505324 -76.505324 37.248884 37.248884 2019-08-19 2019-08-23 Temperate (Mid-Atlantic), Sandy Beach along the York River Estuary, Gloucester Point, Virginia, USA (37.24884N/76.505324W) 0 BCO-DMO catalogue of parameters from In situ tracer injection experiment conducted with 15N-labeled ammonium in a shallow, sandy subterranean estuary in Gloucester Point, USA in August 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/917815.rdf Name: Site_Name Units: unitless Description: <p>Site name where experiment was conducted</p> http://lod.bco-dmo.org/id/dataset-parameter/917816.rdf Name: Latitude Units: decimal degrees Description: <p>Latitude of experiment site location</p> http://lod.bco-dmo.org/id/dataset-parameter/917817.rdf Name: Longitude Units: decimal degrees Description: <p>Longitude of experiment site location</p> http://lod.bco-dmo.org/id/dataset-parameter/917818.rdf Name: Piezometer Units: unitless Description: <p>The ID of the piezometer that was sampled written as replicate cluster-injection site or trace site-depth in cm; (e.g., 1-I-50 is the first replicate cluster, injection site, 50cm depth)</p> http://lod.bco-dmo.org/id/dataset-parameter/917819.rdf Name: Sample_ID Units: unitless Description: <p>The ID of the piezometer that was sampled written as replicate cluster-injection site or trace site-depth in cm-timepoint; (e.g., 1-I-50-T1 is the first replicate cluster, injection site, 50cm depth, time point 1 )</p> http://lod.bco-dmo.org/id/dataset-parameter/917820.rdf Name: Time_Point Units: unitless Description: <p>Injection experiment time point (BKGD, T0, T1, T2, etc). BKGD means background samples, so samples that were collected prior to the start of the injection.</p> http://lod.bco-dmo.org/id/dataset-parameter/917821.rdf Name: Date Units: unitless Description: <p>Date of sample collection</p> http://lod.bco-dmo.org/id/dataset-parameter/917822.rdf Name: Time Units: unitless Description: <p>Time of sample collection</p> http://lod.bco-dmo.org/id/dataset-parameter/917823.rdf Name: Hours_after_Injection Units: hours (hrs) Description: <p>Hours after injectate was introduced in the subsurface</p> http://lod.bco-dmo.org/id/dataset-parameter/917824.rdf Name: Bromide_uM Units: micromoles per liter (uM) Description: <p>Bromide concentration in porewater in micromoles per liter (uM) measured by ion chromatograph</p> http://lod.bco-dmo.org/id/dataset-parameter/917825.rdf Name: Chloride_mM Units: millimoles per liter (mM Description: <p>Chloride concentration in porewater in millimoles per liter (mM) measured by ion chromatograph</p> http://lod.bco-dmo.org/id/dataset-parameter/917826.rdf Name: SF6_ppbv Units: parts per billion per volume (ppbv) Description: <p>Sulfur hexaflouride concentration in porewater in parts per billion per volume (ppbv) measured by gas chromatograph-electron capture detector</p> http://lod.bco-dmo.org/id/dataset-parameter/917827.rdf Name: SF6_pM Units: picoMolar (pM) Description: <p>Sulfur hexaflouride concentration in porewater in picoMolar (pM) measured by gas chromatograph-electron capture detector</p> http://lod.bco-dmo.org/id/dataset-parameter/917828.rdf Name: Nox_uM Units: micromoles per liter (uM) Description: <p>Nitrate + Nitrite concentration in porewater in micromoles per liter (uM) measured by a lachat autoanalyzer</p> http://lod.bco-dmo.org/id/dataset-parameter/917829.rdf Name: NO2_uM Units: micromoles per liter (uM) Description: <p>Nitrite concentration in porewater in micromoles per liter (uM) measured by a lachat autoanalyzer</p> http://lod.bco-dmo.org/id/dataset-parameter/917830.rdf Name: NH4_uM Units: micromoles per liter (uM) Description: <p>Ammonium concentration in porewater in micromoles per liter (uM) measured by a lachat autoanalyzer</p> http://lod.bco-dmo.org/id/dataset-parameter/917831.rdf Name: True_d15N Units: parts per thousand (‰) Description: <p>Delta 15N measurement made by an isotope ratio mass spectrometer</p> http://lod.bco-dmo.org/id/dataset-parameter/917832.rdf Name: Mole_Fraction_Calc Units: unitless Description: <p>15N Mole Fraction calculated from the d15N value</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 9731 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/8db02292-0378-4d05-8949-001ab43a0361/content download https://doi.org/10.26008/1912/bco-dmo.917767.1 download onLine dataset &lt;p&gt;These data were collected during August 2019 from a sandy subterranean estuary (STE) located in Gloucester Point, Virginia, USA. Porewater was collected from surface water and piezometers placed at 40, 50, and 60cm. Prior to the injection, porewater was collected from replicate 50cm piezometers and amended with 15N-labeled ammonium, sulfur hexaflouride, and bromide. The amended porewater was then reintroduced into the STE through the 50cm injection piezometers.&lt;/p&gt; &lt;p&gt;After injection porewater was collected overtime from both the injection piezometers and other piezometers surrounding the injection well. At each time point, porewater was collected in serum bottles prepared with potassium hydroxide and were equlibrated by shaking before being run on a gas chromatograph with electron capture detection (GC-ECD, Shimadzu) in order to determine the sulfur hexaflouride concentration. Porewater was also collected for inorganic nutrients and ions, filtered with a 0.45 µm syringe filter (Whatman GE) and were frozen (-20 °C) until analysis.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;Porewater nutrient concentrations were analyzed for NOx (nitrate + nitrite), nitrite, and ammonium with a Lachat autoanalyzer ions were assessed with an ion chromatograph (ThermoFisher Scientific). The isotopic enrichment of nitrate and nitrite products were measured using a gas bench isotope ratio mass spectrometer (IRMS, Delta V Plus, Thermo Fisher Scientific, Waltham, MA) using the bacterial reduction method. A culture of Pseudomonas aureofaciens reduced NOx in collected samples to N2O. The isotopic composition of the N2O was measured with isotope-ratio mass spectrometry (IRMS) (Sigman et al., 2001).&lt;/p&gt; Specified by the Principal Investigator(s) * Split longitude and latitude of experiment location in separate columns * Reworked Date/Time columns Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Ion Chromatograph, ThermoFisher Scientific; Lachat QuikChem 8000 automated ion analyzer Lachat In- struments Ion Chromatograph, ThermoFisher Scientific; Lachat QuikChem 8000 automated ion analyzer Lachat In- struments PI Supplied Instrument Name: Ion Chromatograph, ThermoFisher Scientific; Lachat QuikChem 8000 automated ion analyzer Lachat In- struments PI Supplied Instrument Description:Ion Chromatograph, ThermoFisher Scientific; Lachat QuikChem 8000 automated ion analyzer Lachat In- struments, Milwaukee, WI, USA; detection limits for NO3−, NH4+, and PO43− are 0.20, 0.36, and 0.16 μM, respectively. Gas bench isotope ratio mass spectrometer (IRMS) Delta V Plus, Thermo Fisher Scientific, Waltham, MA. Instrument Name: Lachat QuikChem 8000 flow injection analyzer and Ion Chromatography (IC) system Instrument Short Name: Instrument Description: The Lachat QuikChem 8000 can operate flow injection analysis and ion chromatography simultaneously and independently on the same instrument platform. Instrument includes sampler, dilutor, sampling pump, electronics unit, and data station. Analysis takes 20-60 seconds, with a sample throughput of 60-120 samples per hour. Measurements are in the range of parts per trillion to parts per hundred.