http://lod.bco-dmo.org/id/dataset/917954
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-01-09
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Thalassia testudinum belowground carbohydrate measurements from experimental plots in the Western Atlantic from Winter 2019 (Tropicalization Seagrass Beds project)
2024-01-09
publication
2024-01-09
revision
BCO-DMO Linked Data URI
2024-01-09
creation
http://lod.bco-dmo.org/id/dataset/917954
Justin Campbell
Florida International University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Campbell, J. (2024) Thalassia testudinum belowground carbohydrate measurements from experimental plots in the Western Atlantic from Winter 2019 (Tropicalization Seagrass Beds project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-01-09 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/917954 [access date]
Thalassia testudinum belowground carbohydrate measurements from experimental plots in the Western Atlantic Dataset Description: Methods and Sampling: <p><strong>Sampling details</strong></p>
<p>Small seagrass plots (0.25m2, all comprised of one species, Thalassia testudinum) were established at 9 shallow sites in the western Atlantic. Each plot was assigned to one of two treatments (n=5/treatment): (1) control plot or (2) plots with the canopy fully clipped. Plots were maintained for 12 months, and at the end, seagrass biomass cores (15cm in diameter) were used to harvest belowground plant material in each plot. Belowground biomass (rhizomes only) were separated from all other material, dried to a constant weight in a 60C oven and ground to a fine powder.&nbsp;This belowground material was then sent to the UC Davis plant lab for the analysis of total soluble carbohydrates (glucose, fructose, sucrose) following the protocols of Smith (1969).</p>
<p>&nbsp;</p>
<p><strong>Total Glucose for Total Nonstructural Carbohydrate (TNC) and Startch Methods Summary</strong></p>
<p>The dataset shows soluble carbohydrates expressed as a percentage of belowground dry mass.&nbsp;Nonstructural carbohydrates are those that can be accumulated and then readily mobilized in order to be metabolized or translocated to other plant parts. This method quantitatively determines the amount of the total glucose following enzymatic hydrolysis. Total non-structural carbohydrates (TNC) is the sum of total glucose, free fructose and free sucrose. Starch is the total glucose minus the free glucose multiplied by 0.9. The free carbohydrates are determined by a separate analysis. The samples for total glucose are enzymatically hydrolyzed at 55°C with amyloglucosidase for 12 hours and analyzed by HPLC with mass selective detection. The analysis uses a Phenomenex Luna NH2 (250 mm x 4.6 mm) HPLC column at a flow rate of 2.75 mL min-1 acetonitrile:water (78:22).</p>
<p>The method has a detection limit of 0.5% and is reproducible to within 10% (relative).</p>
<p>Smith, Dale. Removing and Analyzing Total Nonstructural Carbohydrates from Plant Tissue. Wisconsin Agric. Exp. Sta. Res. Report 41. 1969.</p>
<p>&nbsp;</p>
<p><strong>Site location details</strong></p>
<p>ANDR:&nbsp;Andros, Bahamas (24°53'54.3" N,&nbsp;77°54'25.2" W)</p>
<p>BERM:&nbsp;Bailey's Bay, Bermuda (32°15'49.9" N,&nbsp;64°49'50.5" W)</p>
<p>BOCA:&nbsp;Bocas Del Toro, Panama (9°21'05.8" N,&nbsp;82°15'27.8" W)</p>
<p>BONA:&nbsp;Lac Bay, Bonaire (12°06'44.3" N,&nbsp;68°13'42.0" W)</p>
<p>CARR:&nbsp;Carrie Bow Cay, Belize (16°49'24.8" N,&nbsp;88°06'16.2" W)</p>
<p>CAYM:&nbsp;Little Cayman, Cayman Islands (19°40'14.6" N,&nbsp;80°03'21.3" W)</p>
<p>CORP:&nbsp;Corpus Christi, TX (27°55'47.9" N,&nbsp;97°02'05.2" W)</p>
<p>CRYS:&nbsp;Crystal River, FL (28°42'50.4" N,&nbsp;82°49'08.4" W)</p>
<p>ELEU:&nbsp;Eleuthera, Bahamas (25°27'53.5" N,&nbsp;76°37'35.8" W)</p>
<p>GALV: Galveston, TX (29°02'41.8" N,&nbsp;95°10'15.7" W)</p>
<p>JOES:&nbsp;St. Joe's Bay, FL (29°42'05.0" N,&nbsp;85°19'34.6" W)</p>
<p>NAPL: Naples, FL (26°29'48.6" N,&nbsp;82°09'40.0" W)</p>
<p>PUER: Puerto Morelos, Mexico (20°52'04.5" N,&nbsp;86°51'35.4" W)</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1737247 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1737247
onGoing
Justin Campbell
Florida International University
305-348-2201
3000 NE 151st Street MSB 350
North Miami
FL
33181
USA
jcampbel@fiu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
site
latitude
longitude
season
year
plot
clipping
nutrients
cage
glucose_percentage
sucrose_percentage
fructose_percentage
total_nonstructural_carbohydrates_percentage
Oven
Phenomenex Luna NH2 HPLC Column
theme
None, User defined
site
latitude
longitude
season
year
Site_ID
treatment
carbohydrates
featureType
BCO-DMO Standard Parameters
Drying Oven
Ion Chromatograph
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: The tropicalization of Western Atlantic seagrass beds
https://marinegeo.si.edu/research/research-in-action/underwater-meadows-and-resilient-seas
Collaborative Research: The tropicalization of Western Atlantic seagrass beds
<p><em>NSF Award Abstract:</em><br />
The warming of temperate marine communities is becoming a global phenomenon, producing new biotic interactions that can result in a series of cascading effects on ecosystem structure. For example, the poleward expansion of herbivore populations can lead to the consumption of habitat-forming vegetation, which alters the ecological services provided by coastal environments (a phenomenon known as tropicalization). Many of the habitats at risk, such as kelp forest and seagrass beds, provide foundational habitat that supports complex food webs. Seagrass meadows along the Gulf of Mexico are currently experiencing an influx of tropical grazers, however a integrated understanding of how these communities might ultimately respond is lacking. This project describes the first experiment to quantify the disruptive effect of tropicalization on the ecology of a widely-distributed seagrass. A major contribution of this project will be the development of a seagrass research collaborative network to serve as a platform for broader scientific inquiry and future collaboration. The collaboration spans a total of 11 institutions, and this network will foster extensive collaborations among junior and senior scientists, as well as many undergraduate and graduate students. Given the geographic scope of this work, the research team will further pursue outreach opportunities across the network by hosting a series of public lectures and science café events promoting topics in marine ecology and conservation.</p>
<p>This study will develop a large-scale manipulative experiment across the Caribbean, premised upon a comparative network of 15 marine sites, which will quantify how temperature and light interact with grazer effects on the dominant tropical seagrass, Thalassia testudinum. Sites have been selected along a latitudinal gradient (from Bermuda to Panama), such that light and temperature vary, allowing the investigators to test for the effects of abiotic factors on the ecological effects of increased grazing (tropicalization simulated via artificial leaf clipping). At each of the 15 marine sites, grazing treatments will be crossed with nutrient manipulations in a factorial design for 18 weeks, after which seagrass structure and functioning will be assessed via measurements of areal productivity, shoot density, aboveground biomass, and carbohydrate storage. Experiments will be conducted both in the summer and winter seasons, when abiotic gradients are at their weakest and strongest, respectively. Emerging statistical techniques in hierarchical mixed modeling and structural equation modeling will further allow for integration of experimental and observational data.</p>
Tropicalization Seagrass Beds
largerWorkCitation
project
eng; USA
oceans
-95.171
-64.8307
9.35161
32.2639
2019-02-01
2019-03-31
Western Atlantic
0
BCO-DMO catalogue of parameters from Thalassia testudinum belowground carbohydrate measurements from experimental plots in the Western Atlantic from Winter 2019 (Tropicalization Seagrass Beds project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/919808.rdf
Name: site
Units: unitless
Description: <p>Site name / location; ANDR = Andros, Bahamas, BERM = Bailey's Bay, Bermuda, BOCA = Bocas Del Toro, Panama, BONA = Lac Bay, Bonaire, CARR = Carrie Bow Cay, Belize, CAYM = Little Cayman, Cayman Islands, CORP = Corpus Christi, Texas, CRYS = Crystal River, FL, ELEU = Eleuthera, Bahamas, GALV = Galveston, TX, JOES = St. Joe's Bay, FL, NAPL = Naples, FL, PUER = Puerto Morelos, Mexico</p>
http://lod.bco-dmo.org/id/dataset-parameter/919809.rdf
Name: latitude
Units: decimal degrees
Description: <p>Site latitude in decimal degrees; a positive value indicates a Northern coordinate</p>
http://lod.bco-dmo.org/id/dataset-parameter/919810.rdf
Name: longitude
Units: decimal degrees
Description: <p>Site longitude in decimal degrees; a negative value indicates a Western coordinate</p>
http://lod.bco-dmo.org/id/dataset-parameter/919813.rdf
Name: season
Units: unitless
Description: <p>Season sample collection took place</p>
http://lod.bco-dmo.org/id/dataset-parameter/919814.rdf
Name: year
Units: unitless
Description: <p>Year sample collection took place</p>
http://lod.bco-dmo.org/id/dataset-parameter/919815.rdf
Name: plot
Units: unitless
Description: <p>Plot ID number</p>
http://lod.bco-dmo.org/id/dataset-parameter/919816.rdf
Name: clipping
Units: unitless
Description: <p>Level of seagrass plot canopy clipping; no = no clipping, partial = half canopy removed, full = full canopy removed</p>
http://lod.bco-dmo.org/id/dataset-parameter/919817.rdf
Name: nutrients
Units: unitless
Description: <p>Level of nutrients; ambient=no added nutrients, enriched=added nutrients</p>
http://lod.bco-dmo.org/id/dataset-parameter/919818.rdf
Name: cage
Units: unitless
Description: <p>Level of cage; no=no cage, partial=partial 4-sided cage,full=full cage</p>
http://lod.bco-dmo.org/id/dataset-parameter/919819.rdf
Name: glucose_percentage
Units: unitless
Description: <p>Soluble carbohydrates (glucose) expressed as a percentage of belowground dry mass (%)</p>
http://lod.bco-dmo.org/id/dataset-parameter/919820.rdf
Name: sucrose_percentage
Units: unitless
Description: <p>Soluble carbohydrates (sucrose) expressed as a percentage of belowground dry mass (%)</p>
http://lod.bco-dmo.org/id/dataset-parameter/919821.rdf
Name: fructose_percentage
Units: unitless
Description: <p>Soluble carbohydrates (fructose) expressed as a percentage of belowground dry mass (%)</p>
http://lod.bco-dmo.org/id/dataset-parameter/919822.rdf
Name: total_nonstructural_carbohydrates_percentage
Units: unitless
Description: <p>Non-structural carbohydrates percentage total (%)</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/917954/data/download
download
onLine
dataset
<p><strong>Sampling details</strong></p>
<p>Small seagrass plots (0.25m2, all comprised of one species, Thalassia testudinum) were established at 9 shallow sites in the western Atlantic. Each plot was assigned to one of two treatments (n=5/treatment): (1) control plot or (2) plots with the canopy fully clipped. Plots were maintained for 12 months, and at the end, seagrass biomass cores (15cm in diameter) were used to harvest belowground plant material in each plot. Belowground biomass (rhizomes only) were separated from all other material, dried to a constant weight in a 60C oven and ground to a fine powder.&nbsp;This belowground material was then sent to the UC Davis plant lab for the analysis of total soluble carbohydrates (glucose, fructose, sucrose) following the protocols of Smith (1969).</p>
<p>&nbsp;</p>
<p><strong>Total Glucose for Total Nonstructural Carbohydrate (TNC) and Startch Methods Summary</strong></p>
<p>The dataset shows soluble carbohydrates expressed as a percentage of belowground dry mass.&nbsp;Nonstructural carbohydrates are those that can be accumulated and then readily mobilized in order to be metabolized or translocated to other plant parts. This method quantitatively determines the amount of the total glucose following enzymatic hydrolysis. Total non-structural carbohydrates (TNC) is the sum of total glucose, free fructose and free sucrose. Starch is the total glucose minus the free glucose multiplied by 0.9. The free carbohydrates are determined by a separate analysis. The samples for total glucose are enzymatically hydrolyzed at 55°C with amyloglucosidase for 12 hours and analyzed by HPLC with mass selective detection. The analysis uses a Phenomenex Luna NH2 (250 mm x 4.6 mm) HPLC column at a flow rate of 2.75 mL min-1 acetonitrile:water (78:22).</p>
<p>The method has a detection limit of 0.5% and is reproducible to within 10% (relative).</p>
<p>Smith, Dale. Removing and Analyzing Total Nonstructural Carbohydrates from Plant Tissue. Wisconsin Agric. Exp. Sta. Res. Report 41. 1969.</p>
<p>&nbsp;</p>
<p><strong>Site location details</strong></p>
<p>ANDR:&nbsp;Andros, Bahamas (24°53'54.3" N,&nbsp;77°54'25.2" W)</p>
<p>BERM:&nbsp;Bailey's Bay, Bermuda (32°15'49.9" N,&nbsp;64°49'50.5" W)</p>
<p>BOCA:&nbsp;Bocas Del Toro, Panama (9°21'05.8" N,&nbsp;82°15'27.8" W)</p>
<p>BONA:&nbsp;Lac Bay, Bonaire (12°06'44.3" N,&nbsp;68°13'42.0" W)</p>
<p>CARR:&nbsp;Carrie Bow Cay, Belize (16°49'24.8" N,&nbsp;88°06'16.2" W)</p>
<p>CAYM:&nbsp;Little Cayman, Cayman Islands (19°40'14.6" N,&nbsp;80°03'21.3" W)</p>
<p>CORP:&nbsp;Corpus Christi, TX (27°55'47.9" N,&nbsp;97°02'05.2" W)</p>
<p>CRYS:&nbsp;Crystal River, FL (28°42'50.4" N,&nbsp;82°49'08.4" W)</p>
<p>ELEU:&nbsp;Eleuthera, Bahamas (25°27'53.5" N,&nbsp;76°37'35.8" W)</p>
<p>GALV: Galveston, TX (29°02'41.8" N,&nbsp;95°10'15.7" W)</p>
<p>JOES:&nbsp;St. Joe's Bay, FL (29°42'05.0" N,&nbsp;85°19'34.6" W)</p>
<p>NAPL: Naples, FL (26°29'48.6" N,&nbsp;82°09'40.0" W)</p>
<p>PUER: Puerto Morelos, Mexico (20°52'04.5" N,&nbsp;86°51'35.4" W)</p>
Specified by the Principal Investigator(s)
- removed units from column headers
- converted latitude and longitude columns from degrees, minutes, seconds to decimal degrees
- latitude and longitude values rounded to 6 degrees of precision
- spaces removed from column names and replaced with underscores ("_")
- special characters removed from column names
- split the season field ("Spring 2019") into two separate fields for season ("Spring") and year ("2019")
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Oven
Oven
PI Supplied Instrument Name: Oven PI Supplied Instrument Description:For initial processing, belowground biomass was dried in a 60 C oven. Instrument Name: Drying Oven Instrument Short Name: Instrument Description: a heated chamber for drying
Phenomenex Luna NH2 HPLC Column
Phenomenex Luna NH2 HPLC Column
PI Supplied Instrument Name: Phenomenex Luna NH2 HPLC Column PI Supplied Instrument Description:Determination of soluble carbohydrates was completed with a Phenomenex Luna NH2 (250 mm x 4.6 mm) HPLC.
Instrument Name: Ion Chromatograph Instrument Short Name:Ion Chromatograph Instrument Description: Ion chromatography is a form of liquid chromatography that measures concentrations of ionic species by separating them based on their interaction with a resin. Ionic species separate differently depending on species type and size. Ion chromatographs are able to measure concentrations of major anions, such as fluoride, chloride, nitrate, nitrite, and sulfate, as well as major cations such as lithium, sodium, ammonium, potassium, calcium, and magnesium in the parts-per-billion (ppb) range. (from http://serc.carleton.edu/microbelife/research_methods/biogeochemical/ic.html)