http://lod.bco-dmo.org/id/dataset/918841
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-01-30
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Diatom (Thalassiosira pseudonana) physiological data from experiments designed to study single-cell transcriptional profiling of nutrient acquisition heterogeneity in diatoms conducted in December of 2022
2024-01-30
publication
2024-01-30
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-02-01
publication
https://doi.org/10.26008/1912/bco-dmo.918841.1
Monica V. Orellana
University of Washington Friday Harbor Laboratories
principalInvestigator
Christopher Lausted
Institute for Systems Biology
principalInvestigator
Sui Huang
Institute for Systems Biology
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Orellana, M. V., Lausted, C., Huang, S. (2024) Diatom (Thalassiosira pseudonana) physiological data from experiments designed to study single-cell transcriptional profiling of nutrient acquisition heterogeneity in diatoms conducted in December of 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-01-30 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.918841.1 [access date]
Methods and Sampling: <p dir="ltr"><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt">Cultures: Axenic </span><em>Thalassiosira pseudonana</em><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"> (CCMP 1335, National Center for Marine Algae and Microbiota, Maine, USA; LSID </span>urn:lsid:marinespecies.org:taxname:148934<span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt">) batch cultures were grown in enriched artificial seawater (ESAW) medium modified with reduced levels of nitrate (170 μM) to characterize starvation. Before the experiment, the diatoms were acclimated to a constant 20 °C temperature and under a 12: 12 h dark: light diurnal cycle at 300 μmol photons·m<span style="font-size:0.6em">−2</span></span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt">·s</span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"><span style="font-size:0.6em">−1</span></span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"> using cool fluorescent lights. The cultures were continuously equilibrated at ambient (420 ppm CO</span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"><span style="font-size:0.6em">2</span></span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"> by bubbling mixed gasses (air and CO</span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"><span style="font-size:0.6em">2</span></span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"> at 0.4 L/min) regulated using mass-flow controllers (GFC17, Aalborg) and monitored with a CO</span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"><span style="font-size:0.6em">2</span></span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"> analyzer (model Q-S151; Qubit Systems) into a 1.5-L glass bioreactor system. The bioreactors were inoculated with 150,000 cells/ml of acclimated, axenic </span><em>T. pseudonana</em><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"> and grown for 5 days on a 12: 12 h dark: light cycle. The pH was monitored spectrophotometrically (Dickson et al. 2007); the photochemical yield of photosystem II (variable fluorescence/maximum fluorescence Fv/Fm) was measured with an AquaPen AP100; and total dissolved nitrogen was measure using the Nitrite/Nitrate, Colorimetric Test Roche # 11746081001) kit after syringe-filtering (0.2μm) the samples. Samples were taken twice a day, in the middle of dark-time, and the middle of the light-time representing different growth conditions regulating the metabolism based on light and nitrogen on days one and two (Ashworth et al. 2013). On days three and four, samples were only taken during the light time for experiment b. This experiment was repeated to evaluate the recovery of the cells from starvation by amending NO</span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"><span style="font-size:0.6em">3</span></span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"> (170 uM+/- 5uM) on day 5, after 70 hrs of starvation for </span><em>T. pseudonana</em><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"> and sampled at T1: 0h, T2: 1h and T3: 3 hr, and T4: 6 hr after adding the supplemental nitrogen. Samples T1, T2, T3, were set on ice in the dark before analysis at when T4 was sampled.<br />
<br />
Experiment Metadata (physiology, gene and cell information):</span></p>
<div>Experiment</div>
<div>Start_Date 12-4</div>
<div>Light:Dark 12:12</div>
<div>Lights_On 1:00 PM</div>
<div>Lights_(umols/m.s) 1000 umoles m-2s-1</div>
<div>CO2_ppm ~420</div>
<div>scRNAseq_Timepoints 18 hours, 30 hours, 42 hours, 54 hours, 68 hours,&nbsp;80 hours, 92 hours</div>
<div>scRNAseq_Timepoint_Labels</div>
<div>Notes scRNA, Full ESAW nitrate limited (170uM). Starting innoculate = 150k/m</div>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2029738 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2029738
completed
Monica V. Orellana
University of Washington Friday Harbor Laboratories
Institute for Systems Biology 401 Terry Avenue North
Seattle
WA
98109
USA
monica.orellana@isbscience.org
pointOfContact
Christopher Lausted
Institute for Systems Biology
(206) 732-1276
clausted@isbscience.org
pointOfContact
Sui Huang
Institute for Systems Biology
(206) 732-1208
401Terry Ave North
Seattle
WA
98109
United States
sui.huang@isbscience.org
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Species
Identification
Date
Time
ISO_DateTime_UTC
Hours
Diel
PPM_CO2_analyzer
cell_counts_q1
cell_counts_q2
cell_counts_q3
cell_counts_q4
dF
cells_mL_avgerage
Quantum_yield_1
Quantum_yield_2
Quantum_Yield_3
QY_AVG
FT1
FT2
FT3
FT_AVG
pH
Sample_for_RNA
Cell_Pellet
CF_Media
uM_Nitrate1
uM_Nitrate2
uM_Nitrate3
uM_Nitrate_AVG
CO2 analyzer (model Q-S151; Qubit Systems)
AquaPen AP100, Photon Systems Instruments (Drásov, Czechia)
theme
None, User defined
species scientific name (binomial)
sample description
date
time of day
ISO_DateTime_UTC
time_elapsed
carbon dioxide
count
cell_concentration
quantum yield
fluorescence
pH
Nitrate
featureType
BCO-DMO Standard Parameters
CO2 Analyzer
Fluorometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Ocean Carbon and Biogeochemistry
http://us-ocb.org/
Ocean Carbon and Biogeochemistry
The Ocean Carbon and Biogeochemistry (OCB) program focuses on the ocean's role as a component of the global Earth system, bringing together research in geochemistry, ocean physics, and ecology that inform on and advance our understanding of ocean biogeochemistry. The overall program goals are to promote, plan, and coordinate collaborative, multidisciplinary research opportunities within the U.S. research community and with international partners. Important OCB-related activities currently include: the Ocean Carbon and Climate Change (OCCC) and the North American Carbon Program (NACP); U.S. contributions to IMBER, SOLAS, CARBOOCEAN; and numerous U.S. single-investigator and medium-size research projects funded by U.S. federal agencies including NASA, NOAA, and NSF.
The scientific mission of OCB is to study the evolving role of the ocean in the global carbon cycle, in the face of environmental variability and change through studies of marine biogeochemical cycles and associated ecosystems.
The overarching OCB science themes include improved understanding and prediction of: 1) oceanic uptake and release of atmospheric CO2 and other greenhouse gases and 2) environmental sensitivities of biogeochemical cycles, marine ecosystems, and interactions between the two.
The OCB Research Priorities (updated January 2012) include: ocean acidification; terrestrial/coastal carbon fluxes and exchanges; climate sensitivities of and change in ecosystem structure and associated impacts on biogeochemical cycles; mesopelagic ecological and biogeochemical interactions; benthic-pelagic feedbacks on biogeochemical cycles; ocean carbon uptake and storage; and expanding low-oxygen conditions in the coastal and open oceans.
OCB
largerWorkCitation
program
EAGER: Diatom Programmed Cell Death at Single-Cell Resolution
https://osprey.bco-dmo.org/project/857291
EAGER: Diatom Programmed Cell Death at Single-Cell Resolution
<p><em>NSF Award Abstract:</em><br />
Diatoms are important primary producers in sunlit oceans and lakes across the globe. They are key players in spring phytoplankton blooms, which in turn support food webs that include productive fisheries. In addition, these photosynthetic diatom cells are known to capture enormous amounts of carbon, which are exported to depth as blooms die off. In oceanic systems, this process effectively removes carbon from the atmosphere for thousands of years. However, the biological mechanisms that lead to carbon sequestration, the transfer of carbon from the atmosphere into the deep ocean, are not well understood. As diatom populations reach the end of the bloom cycle, individual cells start to deteriorate and undergo cell death. The collapse of diatom populations is regarded as a critical point (or tipping point), which can be predicted by understanding the genetic activity of the population. This project seeks to elucidate how environmental change influences diatom cell-death processes. A mechanistic understanding of these cellular processes will elucidate how climate change could alter carbon-removal from the atmosphere and affect ocean productivity. The knowledge and methods developed during this study are applicable across organisms from all domains of life. The broader impacts of this project are focused on high school education and new ideas and approaches for three-dimensional learning opportunities in support of Next Generation Science Standards (NGSS). Specifically, researchers are working with high school educators and partners to teach concepts of systems approaches, tipping points, and carbon sequestration in the marine environment through educational modules.</p>
<p>The goal is to determine the structure of diatom populations during the transition of actively growing cells towards population collapse by identifying the point of commitment (tipping point) at the level of individual cells. The model system is the diatom, Thalassiosira pseudonana, which is widespread and a common bloom-forming species. The random decision process of individual diatom cells is being characterized by establishing a genome-wide gene expression space through the physical and biochemical characterization of the cells. Implementation of the project is focused on measuring the phenotypic responses in the diatom to environmental factors including severe stress. Transcriptomic analyses during the transition of cell proliferation towards culture collapse include bulk (RNA-Seq) and single-cell (scRNA-Seq) high-throughput sequencing. Using a systems biology approach, the genetic information obtained from the samples is incorporated into predictive models to identify genetic transitions that occur prior to population collapse. The systems approach can detect changes in transcriptomic state that precede a critical point in the cell death process leading to predictions of how diatoms respond to environmental change. This work opens new vistas for the elucidation of mechanistic pathways of diatom cell populations.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Diatom Death
largerWorkCitation
project
eng; USA
oceans
2022-12-04
2022-12-09
Laboratory study
0
BCO-DMO catalogue of parameters from Diatom (Thalassiosira pseudonana) physiological data from experiments designed to study single-cell transcriptional profiling of nutrient acquisition heterogeneity in diatoms conducted in December of 2022
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/919106.rdf
Name: Species
Units: unitless
Description: <p>Thaps = Thalassiosira pseudonana (LSID urn:lsid:marinespecies.org:taxname:148934)</p>
http://lod.bco-dmo.org/id/dataset-parameter/919107.rdf
Name: Identification
Units: unitless
Description: <p>identification "1"</p>
http://lod.bco-dmo.org/id/dataset-parameter/919108.rdf
Name: Date
Units: unitless
Description: <p>Date sampled (mm-dd-yy)</p>
http://lod.bco-dmo.org/id/dataset-parameter/919109.rdf
Name: Time
Units: unitless
Description: <p>Time sampled (HH:MM), 24hr</p>
http://lod.bco-dmo.org/id/dataset-parameter/919110.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>DateTime with timezone sampled, ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/919111.rdf
Name: Hours
Units: unitless
Description: <p>Hours since innoculation</p>
http://lod.bco-dmo.org/id/dataset-parameter/919112.rdf
Name: Diel
Units: unitless
Description: <p>Diel Cycle (Day/ Night)</p>
http://lod.bco-dmo.org/id/dataset-parameter/919113.rdf
Name: PPM_CO2_analyzer
Units: parts per million (ppm)
Description: <p>CO2 (CO2 Analyzer)</p>
http://lod.bco-dmo.org/id/dataset-parameter/919114.rdf
Name: cell_counts_q1
Units: cells
Description: <p>Cell counts q1 (quadrant 1)</p>
http://lod.bco-dmo.org/id/dataset-parameter/919115.rdf
Name: cell_counts_q2
Units: cells
Description: <p>Cell counts q2 (quadrant 2)</p>
http://lod.bco-dmo.org/id/dataset-parameter/919116.rdf
Name: cell_counts_q3
Units: cells
Description: <p>Cell counts q3 (quadrant 3)</p>
http://lod.bco-dmo.org/id/dataset-parameter/919117.rdf
Name: cell_counts_q4
Units: cells
Description: <p>Cell counts q4 (quadrant 4)</p>
http://lod.bco-dmo.org/id/dataset-parameter/919118.rdf
Name: dF
Units: unitless
Description: <p>Dilution factor</p>
http://lod.bco-dmo.org/id/dataset-parameter/919119.rdf
Name: cells_mL_avgerage
Units: cells per ml (cells/ml)
Description: <p>cell concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/919120.rdf
Name: Quantum_yield_1
Units: unitless
Description: <p>Quantum Yield replicate 1</p>
http://lod.bco-dmo.org/id/dataset-parameter/919121.rdf
Name: Quantum_yield_2
Units: unitless
Description: <p>Quantum Yield replicate 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/919122.rdf
Name: Quantum_Yield_3
Units: unitless
Description: <p>Quantum Yield replicate 3</p>
http://lod.bco-dmo.org/id/dataset-parameter/919123.rdf
Name: QY_AVG
Units: unitless
Description: <p>Quantum Yield Average</p>
http://lod.bco-dmo.org/id/dataset-parameter/919124.rdf
Name: FT1
Units: unitless
Description: <p>Fluorescence replicate 1</p>
http://lod.bco-dmo.org/id/dataset-parameter/919125.rdf
Name: FT2
Units: unitless
Description: <p>Fluorescence replicate 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/919126.rdf
Name: FT3
Units: unitless
Description: <p>Fluorescence replicate 3</p>
http://lod.bco-dmo.org/id/dataset-parameter/919127.rdf
Name: FT_AVG
Units: unitless
Description: <p>Fluorescence Average</p>
http://lod.bco-dmo.org/id/dataset-parameter/919128.rdf
Name: pH
Units: unitless
Description: <p>pH (potential for Hydrogen)</p>
http://lod.bco-dmo.org/id/dataset-parameter/919129.rdf
Name: Sample_for_RNA
Units: unitless
Description: <p>Sample for RNA (Yes/No)</p>
http://lod.bco-dmo.org/id/dataset-parameter/919130.rdf
Name: Cell_Pellet
Units: unitless
Description: <p>Cell pellet (Yes/No)</p>
http://lod.bco-dmo.org/id/dataset-parameter/919131.rdf
Name: CF_Media
Units: unitless
Description: <p>Cell free media for nutrient profiling </p>
http://lod.bco-dmo.org/id/dataset-parameter/919132.rdf
Name: uM_Nitrate1
Units: micromolar (uM)
Description: <p>Nitrate replicate 1</p>
http://lod.bco-dmo.org/id/dataset-parameter/919133.rdf
Name: uM_Nitrate2
Units: micromolar (uM)
Description: <p>Nitrate replicate 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/919134.rdf
Name: uM_Nitrate3
Units: micromolar (uM)
Description: <p>Nitrate replicate 3</p>
http://lod.bco-dmo.org/id/dataset-parameter/919135.rdf
Name: uM_Nitrate_AVG
Units: micromolar (uM)
Description: <p>Nitrate average</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2769
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/c8548ae3-1ce3-46da-9397-e2475526acd0/content
download
https://doi.org/10.26008/1912/bco-dmo.918841.1
download
onLine
dataset
<p dir="ltr"><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt">Cultures: Axenic </span><em>Thalassiosira pseudonana</em><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"> (CCMP 1335, National Center for Marine Algae and Microbiota, Maine, USA; LSID </span>urn:lsid:marinespecies.org:taxname:148934<span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt">) batch cultures were grown in enriched artificial seawater (ESAW) medium modified with reduced levels of nitrate (170 μM) to characterize starvation. Before the experiment, the diatoms were acclimated to a constant 20 °C temperature and under a 12: 12 h dark: light diurnal cycle at 300 μmol photons·m<span style="font-size:0.6em">−2</span></span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt">·s</span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"><span style="font-size:0.6em">−1</span></span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"> using cool fluorescent lights. The cultures were continuously equilibrated at ambient (420 ppm CO</span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"><span style="font-size:0.6em">2</span></span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"> by bubbling mixed gasses (air and CO</span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"><span style="font-size:0.6em">2</span></span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"> at 0.4 L/min) regulated using mass-flow controllers (GFC17, Aalborg) and monitored with a CO</span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"><span style="font-size:0.6em">2</span></span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"> analyzer (model Q-S151; Qubit Systems) into a 1.5-L glass bioreactor system. The bioreactors were inoculated with 150,000 cells/ml of acclimated, axenic </span><em>T. pseudonana</em><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"> and grown for 5 days on a 12: 12 h dark: light cycle. The pH was monitored spectrophotometrically (Dickson et al. 2007); the photochemical yield of photosystem II (variable fluorescence/maximum fluorescence Fv/Fm) was measured with an AquaPen AP100; and total dissolved nitrogen was measure using the Nitrite/Nitrate, Colorimetric Test Roche # 11746081001) kit after syringe-filtering (0.2μm) the samples. Samples were taken twice a day, in the middle of dark-time, and the middle of the light-time representing different growth conditions regulating the metabolism based on light and nitrogen on days one and two (Ashworth et al. 2013). On days three and four, samples were only taken during the light time for experiment b. This experiment was repeated to evaluate the recovery of the cells from starvation by amending NO</span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"><span style="font-size:0.6em">3</span></span><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"> (170 uM+/- 5uM) on day 5, after 70 hrs of starvation for </span><em>T. pseudonana</em><span style="background-color:transparent; color:rgb(0, 0, 0); font-family:arial,sans-serif; font-size:11pt"> and sampled at T1: 0h, T2: 1h and T3: 3 hr, and T4: 6 hr after adding the supplemental nitrogen. Samples T1, T2, T3, were set on ice in the dark before analysis at when T4 was sampled.<br />
<br />
Experiment Metadata (physiology, gene and cell information):</span></p>
<div>Experiment</div>
<div>Start_Date 12-4</div>
<div>Light:Dark 12:12</div>
<div>Lights_On 1:00 PM</div>
<div>Lights_(umols/m.s) 1000 umoles m-2s-1</div>
<div>CO2_ppm ~420</div>
<div>scRNAseq_Timepoints 18 hours, 30 hours, 42 hours, 54 hours, 68 hours,&nbsp;80 hours, 92 hours</div>
<div>scRNAseq_Timepoint_Labels</div>
<div>Notes scRNA, Full ESAW nitrate limited (170uM). Starting innoculate = 150k/m</div>
Specified by the Principal Investigator(s)
* Sheet 1 of file "EAGER 10x Physiological metadata and measurements.xlsx" was imported into the BCO-DMO data system up to Excel row 15 (following rows containing metadata describing the columns was not imported as part of the data table, it was added to the "Parameters" section of the dataset metadata.
* DateTime (UTC) added in ISO 8601 format using provided dates and times (PST/PSD) .
* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores. Can not start with a number]
* Three columns for replicates of F/cell flow were removed from table at submitter's request.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
CO2 analyzer (model Q-S151; Qubit Systems)
CO2 analyzer (model Q-S151; Qubit Systems)
PI Supplied Instrument Name: CO2 analyzer (model Q-S151; Qubit Systems) Instrument Name: CO2 Analyzer Instrument Short Name:CO2 Analyzer Instrument Description: Measures atmospheric carbon dioxide (CO2) concentration. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/382/
AquaPen AP100, Photon Systems Instruments (Drásov, Czechia)
AquaPen AP100, Photon Systems Instruments (Drásov, Czechia)
PI Supplied Instrument Name: AquaPen AP100, Photon Systems Instruments (Drásov, Czechia) Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/