http://lod.bco-dmo.org/id/dataset/920383
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-02-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Bottle sample data from CTD casts from R/V Hugh R. Sharp HRS1610 in the Mid-Atlantic coastal waters from August 2016 (CyanateInTheSea project)
2024-02-16
publication
2024-02-16
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-03-19
publication
https://doi.org/10.26008/1912/bco-dmo.920383.1
Margaret Mulholland
Old Dominion University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Mulholland, M. (2024) Bottle sample data from CTD casts from R/V Hugh R. Sharp HRS1610 in the Mid-Atlantic coastal waters from August 2016 (CyanateInTheSea project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-02-16 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.920383.1 [access date]
Bottle sample data from CTD casts Dataset Description: Methods and Sampling: <p>Nutrient samples at each depth were collected directly from Niskin bottles through a 0.2 μm capsule filter (Pall Supor<sup>®</sup>) using a peristaltic pump at pressures ≤5 mm Hg. Filtrate was collected directly into two 50 mL sterile polypropylene centrifuge tubes (Falcon<sup>®</sup>) to measure nitrate (NO<sub>3</sub><sup>–</sup>), nitrite (NO<sub>2</sub><sup>–</sup>), orthophosphate ions (simplified as PO<sub>4</sub>, sum of HPO<sub>4</sub><sup>2–</sup> and PO<sub>4</sub><sup>3–</sup>), and urea concentrations. Filtrate was also pumped into three 2 mL sterile polypropylene tubes for cyanate determinations, two 15 mL OPA-treated polypropylene tubes (Falcon<sup>®</sup>) for ammonium (NH<sub>4</sub><sup>+</sup>) analyses, and two 40 mL combusted amber glass vials for analysis of total dissolved free primary amine. To minimize contamination from filtration and between samples, filters and tubing were rinsed thoroughly with site water before sample collection. NO<sub>3</sub><sup>–</sup>+ NO<sub>2</sub><sup>–</sup>, NO<sub>2</sub><sup>–</sup>, PO<sub>4</sub>,<sub> </sub>and urea samples (duplicates) were stored at 4 °C until analysis within 48 hours of their collection using a nutrient autoanalyzer (Astoria-Pacific, Inc., USA) according to the manufacturer’s specifications. The method detection limits for NO<sub>3</sub><sup>–</sup>+ NO<sub>2</sub><sup>–</sup>, NO<sub>2</sub><sup>–</sup>, PO<sub>4</sub>,<sub> </sub>and urea<sup> </sup>were 0.14 μmol L<sup>–1</sup>, 0.07 μmol L<sup>–1</sup>, 0.03 μmol L<sup>–1</sup>,<sup> </sup>and 0.08 μmol L<sup>–1</sup>, respectively. NH<sub>4</sub><sup>+</sup> samples (duplicates) were kept at 4 °C until analysis within 24 hours of collection. The concentrations of NH<sub>4</sub><sup>+</sup> were measured onboard using the OPA-fluorescence method of Holmes et al. (1999) with a spectrofluorometer. The method detection limit was 10.0 nmol L<sup>–1</sup>. Cyanate samples (triplicates) were stored in liquid nitrogen aboard the ship and at -80 °C once samples were returned to the land-based laboratory. Cyanate concentrations were measured by high-performance liquid chromatography (HPLC) using a precolumn fluorescence derivatization method (Widner et al., 2013; Widner and Mulholland, 2017). The method detection limit was 0.4 nmol L<sup>–1</sup>.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1459698 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1459698
onGoing
Margaret Mulholland
Old Dominion University
757-739-0449
Department of Ocean, Earth, and Atmospheric Sciences 4600 Elkhorn Ave.
Norfolk
VA
23529-0276
USA
mmulholl@odu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Station
Cast
ISO_DateTime_UTC
Latitude
Longitude
Pressure
Depth
Temperature
Salinity
Fluorescence
Density
Density_sigma_t
Nitrate
Phosphate
Nitrite
Ammonium
Cyanate
High-performance liquid chromatography (Shimadzu)
10 L Niskin bottles
Astoria-Pacific, Inc., USA
Peristaltic pump
Spectrofluorometer
theme
None, User defined
station id
cast
ISO_DateTime_UTC
latitude
longitude
water pressure
depth
water temperature at measurement depth
salinity
fluorescence
density
Nitrate
phosphate
Nitrite
Ammonium
Cyanate (CNO)
featureType
BCO-DMO Standard Parameters
High-Performance Liquid Chromatograph
Niskin bottle
Nutrient Autoanalyzer
Pump
Spectrometer
instrument
BCO-DMO Standard Instruments
HRS1610
service
Deployment Activity
Mid-Atlantic coastal waters
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Cyanate in the Sea: Sources, Sinks, and Quantitative Significance
https://osprey.bco-dmo.org/project/683923
Cyanate in the Sea: Sources, Sinks, and Quantitative Significance
<p><strong>NSF Award Abstract:</strong></p>
<p>Nitrogen is a critical nutrient in the world's oceans because, among other things, it is a major component of living organisms and can be a driver in primary productivity. Nitrogen is present in the ocean in a number of organic and inorganic forms, which vary in their ease in being assimilated by marine organisms. Cyanate is a simple form of organic nitrogen present in the ocean, although its abundance and importance to the ocean nitrogen cycle is poorly understood. Using newly developed and tested methods for measuring ambient cyanate concentrations and its uptake in seawater, researchers will analyze the distribution, sources, and geochemistry of cyanate in shelf waters of the Atlantic Ocean. Results from this project will elucidate the importance of cyanate in the marine nitrogen cycle and transform understanding of cyanate production and assimilation in the sea. This project will provide a unique opportunity for both graduate student research and undergraduate training, and will likely expose underrepresented groups to marine sciences.</p>
<p>Although physiological and genomic evidence suggest that marine microbes can utilize a broad array of inorganic and organic nitrogen compounds, cyanate's role in the marine nitrogen cycle has not yet been examined. As one of the simplest organic nitrogen compounds, cyanate has likely been present in the environment over Earth's long history. Evidence suggests that cyanate metabolism appeared early on in bacterial genomes and thus, the study of cyanate assimilation in the contemporary ocean may illuminate microbial processes with deep evolutionary roots. However, a decade since discovering the genomic capacity for cyanate utilization in marine cyanobacteria, little is still known about cyanate distributions in the environment, how it is produced, and how widespread cyanate utilization is among marine microbes. To further understanding of cyanate's role in the marine nitrogen cycle, a combination of geochemical approaches will be used to assess: 1) the distribution of cyanate in the marine environment, 2) potential sources of cyanate and the timescales at which cyanate is produced, 3) the rate of cyanate removal via microbial uptake and spontaneous decomposition, and 4) the geochemical coupling between cyanate production and consumption. Results generated from this study will be important for augmenting knowledge of the marine nitrogen cycle, refining biogeochemical models, and further understanding of the functioning of marine microbial communities.</p>
CyanateInTheSea
largerWorkCitation
project
eng; USA
oceans
Mid-Atlantic coastal waters
-78.5773
-73.3663
33.334
37.668
2016-08-07
2016-08-17
Western North Atlantic Coastal waters (mid- and south Atlantic Bight)
0
BCO-DMO catalogue of parameters from Bottle sample data from CTD casts from R/V Hugh R. Sharp HRS1610 in the Mid-Atlantic coastal waters from August 2016 (CyanateInTheSea project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/922347.rdf
Name: Station
Units: unitless
Description: <p>Station number (1-41)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922348.rdf
Name: Cast
Units: unitless
Description: <p>CTD cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/922349.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Datetime of CTD cast in UTC</p>
http://lod.bco-dmo.org/id/dataset-parameter/922350.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of cast location in decimal degrees; a positive value indicates a Northern coordinate</p>
http://lod.bco-dmo.org/id/dataset-parameter/922351.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of cast location in decimal degrees; a negative value indicates a Western coordinate</p>
http://lod.bco-dmo.org/id/dataset-parameter/922352.rdf
Name: Pressure
Units: decibar (db)
Description: <p>Pressure value from CTD</p>
http://lod.bco-dmo.org/id/dataset-parameter/922353.rdf
Name: Depth
Units: meters (m)
Description: <p>Depth value converted from pressure value</p>
http://lod.bco-dmo.org/id/dataset-parameter/922354.rdf
Name: Temperature
Units: Celcius (C)
Description: <p>Temperature value from CTD</p>
http://lod.bco-dmo.org/id/dataset-parameter/922355.rdf
Name: Salinity
Units: practical salinity unit (PSU)
Description: <p>Salinity value from CTD</p>
http://lod.bco-dmo.org/id/dataset-parameter/922356.rdf
Name: Fluorescence
Units: milligram per cubic meter (mg/m^3)
Description: <p>Fluorescence value from WET Labs ECO-AFL/FL</p>
http://lod.bco-dmo.org/id/dataset-parameter/922357.rdf
Name: Density
Units: kilograms per cubic meter (kg/m^3)
Description: <p>Density value calculated from Gibbs-SeaWater (GSW) quation</p>
http://lod.bco-dmo.org/id/dataset-parameter/922358.rdf
Name: Density_sigma_t
Units: kilograms per cubic meter (kg/m^3)
Description: <p>Density sigma-t value calculated from Gibbs-SeaWater (GSW) equation</p>
http://lod.bco-dmo.org/id/dataset-parameter/922359.rdf
Name: Nitrate
Units: micromole per liter (µmol/L^1)
Description: <p>Nitrate value determined by NO3+NO2 minus NO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/922360.rdf
Name: Phosphate
Units: micromole per liter (µmol/L^1)
Description: <p>Orthophosphate ions</p>
http://lod.bco-dmo.org/id/dataset-parameter/922361.rdf
Name: Nitrite
Units: micromole per liter (µmol/L^1)
Description: <p>Nitrite value measured separately from NO3+NO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/922362.rdf
Name: Ammonium
Units: nanomole per liter (nmol/L^1)
Description: <p>Ammonium value averaged from duplicates</p>
http://lod.bco-dmo.org/id/dataset-parameter/922363.rdf
Name: Cyanate
Units: nanomole per liter (nmol/L^1)
Description: <p>Cyanate averaged from duplicates</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
54840
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/8c0d726a-0143-46b5-a75d-3a04cd1fd852/content
download
https://doi.org/10.26008/1912/bco-dmo.920383.1
download
onLine
dataset
<p>Nutrient samples at each depth were collected directly from Niskin bottles through a 0.2 μm capsule filter (Pall Supor<sup>®</sup>) using a peristaltic pump at pressures ≤5 mm Hg. Filtrate was collected directly into two 50 mL sterile polypropylene centrifuge tubes (Falcon<sup>®</sup>) to measure nitrate (NO<sub>3</sub><sup>–</sup>), nitrite (NO<sub>2</sub><sup>–</sup>), orthophosphate ions (simplified as PO<sub>4</sub>, sum of HPO<sub>4</sub><sup>2–</sup> and PO<sub>4</sub><sup>3–</sup>), and urea concentrations. Filtrate was also pumped into three 2 mL sterile polypropylene tubes for cyanate determinations, two 15 mL OPA-treated polypropylene tubes (Falcon<sup>®</sup>) for ammonium (NH<sub>4</sub><sup>+</sup>) analyses, and two 40 mL combusted amber glass vials for analysis of total dissolved free primary amine. To minimize contamination from filtration and between samples, filters and tubing were rinsed thoroughly with site water before sample collection. NO<sub>3</sub><sup>–</sup>+ NO<sub>2</sub><sup>–</sup>, NO<sub>2</sub><sup>–</sup>, PO<sub>4</sub>,<sub> </sub>and urea samples (duplicates) were stored at 4 °C until analysis within 48 hours of their collection using a nutrient autoanalyzer (Astoria-Pacific, Inc., USA) according to the manufacturer’s specifications. The method detection limits for NO<sub>3</sub><sup>–</sup>+ NO<sub>2</sub><sup>–</sup>, NO<sub>2</sub><sup>–</sup>, PO<sub>4</sub>,<sub> </sub>and urea<sup> </sup>were 0.14 μmol L<sup>–1</sup>, 0.07 μmol L<sup>–1</sup>, 0.03 μmol L<sup>–1</sup>,<sup> </sup>and 0.08 μmol L<sup>–1</sup>, respectively. NH<sub>4</sub><sup>+</sup> samples (duplicates) were kept at 4 °C until analysis within 24 hours of collection. The concentrations of NH<sub>4</sub><sup>+</sup> were measured onboard using the OPA-fluorescence method of Holmes et al. (1999) with a spectrofluorometer. The method detection limit was 10.0 nmol L<sup>–1</sup>. Cyanate samples (triplicates) were stored in liquid nitrogen aboard the ship and at -80 °C once samples were returned to the land-based laboratory. Cyanate concentrations were measured by high-performance liquid chromatography (HPLC) using a precolumn fluorescence derivatization method (Widner et al., 2013; Widner and Mulholland, 2017). The method detection limit was 0.4 nmol L<sup>–1</sup>.</p>
Specified by the Principal Investigator(s)
- Removed row from data containing unit information
- Changed second "Density" column header name to "Density_sigma_t"
- Rounded latitude and longitude values to 6 degrees of precision
- "No data" values in the primary data file are represented by blank values in cells (NAs were removed from the original data presentation)
- Merged separate date and time columns into one datetime column called ISO_DateTime_UTC
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
High-performance liquid chromatography (Shimadzu)
High-performance liquid chromatography (Shimadzu)
PI Supplied Instrument Name: High-performance liquid chromatography (Shimadzu) PI Supplied Instrument Description:A high-performance liquid chromatography was used for cyanate analysis Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/
10 L Niskin bottles
10 L Niskin bottles
PI Supplied Instrument Name: 10 L Niskin bottles PI Supplied Instrument Description:Dissolved seawater samples were collected using a sampling rosette equipped with a 12 X 10 L Niskin bottles. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Astoria-Pacific, Inc., USA
Astoria-Pacific, Inc., USA
PI Supplied Instrument Name: Astoria-Pacific, Inc., USA PI Supplied Instrument Description:A nutrient autoanalyzer was used for nutrient measurement Instrument Name: Nutrient Autoanalyzer Instrument Short Name:Nutrient Autoanalyzer Instrument Description: Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
Peristaltic pump
Peristaltic pump
PI Supplied Instrument Name: Peristaltic pump PI Supplied Instrument Description:A peristaltic pump and 0.2 μm capsule filter were used for seawater sample collection Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps
Spectrofluorometer
Spectrofluorometer
PI Supplied Instrument Name: Spectrofluorometer PI Supplied Instrument Description:A spectrofluorometer was used for ammonium determination Instrument Name: Spectrometer Instrument Short Name:Spectrometer Instrument Description: A spectrometer is an optical instrument used to measure properties of light over a specific portion of the electromagnetic spectrum. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0460/
Cruise: HRS1610
HRS1610
R/V Hugh R. Sharp
Community Standard Description
International Council for the Exploration of the Sea
R/V Hugh R. Sharp
vessel
HRS1610
Margaret Mulholland
Old Dominion University
R/V Hugh R. Sharp
Community Standard Description
International Council for the Exploration of the Sea
R/V Hugh R. Sharp
vessel