http://lod.bco-dmo.org/id/dataset/920423
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-02-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Particulate Carbon and Nitrogen data from CTD casts from R/V Hugh R. Sharp HRS1610 in the Mid-Atlantic coastal waters from August 2016 (CyanateInTheSea project)
2024-02-19
publication
2024-02-19
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-03-19
publication
https://doi.org/10.26008/1912/bco-dmo.920423.1
Margaret Mulholland
Old Dominion University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Mulholland, M. (2024) Particulate Carbon and Nitrogen data from CTD casts from R/V Hugh R. Sharp HRS1610 in the Mid-Atlantic coastal waters from August 2016 (CyanateInTheSea project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-02-19 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.920423.1 [access date]
Particulate Carbon and Nitrogen data from CTD casts Dataset Description: Methods and Sampling: <p><strong>Particulate Carbon and Nitrogen (PNPC)</strong></p>
<p>Whole water from three target depths (near surface, the depth above the Chl maximum (or near the bottom of the mixed layer), and the depth of the Chl maximum) at each station was collected from Niskin bottles into 10 L carboys and transported to the on-board laboratory where water samples were mixed and sub-samples (0.2–1.4 L) were collected onto pre-combusted GF-75 filters (Whatman<sup>®</sup>, nominal pore size 0.3 μm) in triplicate, for analysis of particulate nitrogen (PN) and carbon (PC) concentrations and the natural abundance of <sup>15</sup>N and <sup>13</sup>C. The filters were stored in cryovials and immediately frozen and stored in a freezer at -20 °C until analysis. Prior to their analysis, filters were dried at 40 ºC, pelletized in tin capsules, and analyzed on a Europa 20/20 isotope ratio mass spectrometer equipped with an automated N and C analyzer. The detection limit of the mass spectrometer was 0.0018 and 0.0005 atom% for <sup>15</sup>N and <sup>13</sup>C, respectively; these values were derived based on three times the standard deviation (3 × SD) of the atom% of 12.5 µg N and 100 µg C standards (n=40).</p>
<p>&nbsp;</p>
<p><strong>Nitrogen Uptake&nbsp;</strong></p>
<p>Parallel sets of triplicate whole water (0.5−2 L) from the three target depths mentioned above (near surface, above the depth of the Chl maximum (or near the bottom of the mixed layer), and at the depth of the Chl maximum) from 27 stations were dispensed from 10 L carboys into acid-cleaned polyethylene terephthalate glycol incubation bottles (Nalgene<sup>TM</sup>).</p>
<p>Nitrogen uptake incubations were initiated by amending incubation bottles with highly enriched (98−99%) <sup>15</sup>N-labeled substrates (Cambridge Isotope Laboratories, Inc., USA), including ammonium chloride (<sup>15</sup>NH<sub>4</sub>Cl), potassium nitrate (K<sup>15</sup>NO<sub>3</sub>), potassium nitrite (K<sup>15</sup>NO<sub>2</sub>), and <sup>15</sup>N- and <sup>13</sup>C-labeled potassium cyanate (KO<sup>13</sup>C<sup>15</sup>N), urea (<sup>13</sup>CO(<sup>15</sup>NH<sub>2</sub>)<sub>2</sub>), and algal amino acid mixture.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1459698 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1459698
onGoing
Margaret Mulholland
Old Dominion University
757-739-0449
Department of Ocean, Earth, and Atmospheric Sciences 4600 Elkhorn Ave.
Norfolk
VA
23529-0276
USA
mmulholl@odu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Station
Cast
ISO_DateTime_UTC
Latitude
Longitude
Depth
Temp
Salinity
Fluorescence
Total_Chl
Chl_SD
Ammonium
Nitrate
Nitrite
Urea
Phosphate
Dissolved_free_amino_acids
Cyanate
PC
PN
NH4_avg_uptake_rate
SD_NH4
NO3_avg_uptake_rate
SD_NO3
NO2_avg_uptake_rate
SD_NO2
Urea_avg_uptake_rate
SD_Urea
AAs_avg_uptake_rate
SD_AAs
Cyanate_avg_uptake_rate
SD_cyanate
Specific_NH4_uptake_rate
Specific_NO3_uptake_rate
Specific_NO2_uptake_rate
Specific_urea_uptake_rate
Specific_AAs_uptake_rate
Specific_cyanate_uptake_rate
10 L Niskin bottles
theme
None, User defined
station id
cast
ISO_DateTime_UTC
latitude
longitude
depth
water temperature at measurement depth
salinity
fluorescence
chlorophyll a
standard deviation
Ammonium
Nitrate
Nitrite
Urea
phosphate
amino acid concentration
Cyanate (CNO)
total particulate Carbon
Nitrogen
amino acid uptake
cyanate (CNO) uptake
N15 uptake rate
pNO3
Urea uptake rate
featureType
BCO-DMO Standard Parameters
Mass Spectrometer
Niskin bottle
instrument
BCO-DMO Standard Instruments
HRS1610
service
Deployment Activity
Mid-Atlantic coastal waters
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Cyanate in the Sea: Sources, Sinks, and Quantitative Significance
https://osprey.bco-dmo.org/project/683923
Cyanate in the Sea: Sources, Sinks, and Quantitative Significance
<p><strong>NSF Award Abstract:</strong></p>
<p>Nitrogen is a critical nutrient in the world's oceans because, among other things, it is a major component of living organisms and can be a driver in primary productivity. Nitrogen is present in the ocean in a number of organic and inorganic forms, which vary in their ease in being assimilated by marine organisms. Cyanate is a simple form of organic nitrogen present in the ocean, although its abundance and importance to the ocean nitrogen cycle is poorly understood. Using newly developed and tested methods for measuring ambient cyanate concentrations and its uptake in seawater, researchers will analyze the distribution, sources, and geochemistry of cyanate in shelf waters of the Atlantic Ocean. Results from this project will elucidate the importance of cyanate in the marine nitrogen cycle and transform understanding of cyanate production and assimilation in the sea. This project will provide a unique opportunity for both graduate student research and undergraduate training, and will likely expose underrepresented groups to marine sciences.</p>
<p>Although physiological and genomic evidence suggest that marine microbes can utilize a broad array of inorganic and organic nitrogen compounds, cyanate's role in the marine nitrogen cycle has not yet been examined. As one of the simplest organic nitrogen compounds, cyanate has likely been present in the environment over Earth's long history. Evidence suggests that cyanate metabolism appeared early on in bacterial genomes and thus, the study of cyanate assimilation in the contemporary ocean may illuminate microbial processes with deep evolutionary roots. However, a decade since discovering the genomic capacity for cyanate utilization in marine cyanobacteria, little is still known about cyanate distributions in the environment, how it is produced, and how widespread cyanate utilization is among marine microbes. To further understanding of cyanate's role in the marine nitrogen cycle, a combination of geochemical approaches will be used to assess: 1) the distribution of cyanate in the marine environment, 2) potential sources of cyanate and the timescales at which cyanate is produced, 3) the rate of cyanate removal via microbial uptake and spontaneous decomposition, and 4) the geochemical coupling between cyanate production and consumption. Results generated from this study will be important for augmenting knowledge of the marine nitrogen cycle, refining biogeochemical models, and further understanding of the functioning of marine microbial communities.</p>
CyanateInTheSea
largerWorkCitation
project
eng; USA
oceans
Mid-Atlantic coastal waters
-78.5848
-73.3708
33.3306
37.6683
2016-08-07
2016-08-17
Western North Atlantic Coastal waters (mid- and south Atlantic Bight)
0
BCO-DMO catalogue of parameters from Particulate Carbon and Nitrogen data from CTD casts from R/V Hugh R. Sharp HRS1610 in the Mid-Atlantic coastal waters from August 2016 (CyanateInTheSea project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/922526.rdf
Name: Station
Units: unitless
Description: <p>Station number (1-41)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922527.rdf
Name: Cast
Units: unitless
Description: <p>CTD cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/922528.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Datetime of CTD cast in UTC</p>
http://lod.bco-dmo.org/id/dataset-parameter/922529.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of cast location in decimal degrees; a positive value indicates a Northern coordinate</p>
http://lod.bco-dmo.org/id/dataset-parameter/922530.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of cast location in decimal degrees; a negative value indicates a Western coordinate</p>
http://lod.bco-dmo.org/id/dataset-parameter/922531.rdf
Name: Depth
Units: meters (m)
Description: <p>Depth value from CTD</p>
http://lod.bco-dmo.org/id/dataset-parameter/922532.rdf
Name: Temp
Units: Celcius (C)
Description: <p>Temperature value from CTD</p>
http://lod.bco-dmo.org/id/dataset-parameter/922533.rdf
Name: Salinity
Units: practical salinity unit (PSU)
Description: <p>Salinity value from CTD</p>
http://lod.bco-dmo.org/id/dataset-parameter/922534.rdf
Name: Fluorescence
Units: milligram per cubic meter (mg/m^3)
Description: <p>Fluorescence value from WET Labs ECO-AFL/FL</p>
http://lod.bco-dmo.org/id/dataset-parameter/922535.rdf
Name: Total_Chl
Units: microgram per liter (µg/L^1)
Description: <p>Total chlorophyll (chl) value measured through high-performance liquid chromatoraphy (HPLC)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922536.rdf
Name: Chl_SD
Units: microgram per liter (µg/L^1)
Description: <p>Standard deciation of chlorophyll</p>
http://lod.bco-dmo.org/id/dataset-parameter/922537.rdf
Name: Ammonium
Units: micromole per liter (µmol/L^1)
Description: <p>Ambient NH4 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/922538.rdf
Name: Nitrate
Units: micromole per liter (µmol/L^1)
Description: <p>Ambient NO3 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/922539.rdf
Name: Nitrite
Units: micromole per liter (µmol/L^1)
Description: <p>Ambient NO2 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/922540.rdf
Name: Urea
Units: micromole N per liter (µmol N /L^1)
Description: <p>Ambient Urea concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/922541.rdf
Name: Phosphate
Units: micromole per liter (µmol/L^1)
Description: <p>Ambient PO4 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/922542.rdf
Name: Dissolved_free_amino_acids
Units: micromole N per liter (µmol N /L^1)
Description: <p>Ambient Dissloved free amino acids concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/922543.rdf
Name: Cyanate
Units: micromole per liter (µmol/L^1)
Description: <p>Ambient cyanate concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/922544.rdf
Name: PC
Units: micromole per liter (µmol/L^1)
Description: <p>Initial particulate carbon concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/922545.rdf
Name: PN
Units: micromols N per liter (µmol N/L^1)
Description: <p>Initial particulate nitrogen concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/922546.rdf
Name: NH4_avg_uptake_rate
Units: micromols N per liter per hour (µmol N /L^1/h^1)
Description: <p>Ammonium average uptake rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/922547.rdf
Name: SD_NH4
Units: micromols N per liter per hour (µmol N /L^1/h^1)
Description: <p>Standard deviation of ammonium uptake rate (triplicates)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922548.rdf
Name: NO3_avg_uptake_rate
Units: micromols N per liter per hour (µmol N /L^1/h^1)
Description: <p>Nitrate average uptake rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/922549.rdf
Name: SD_NO3
Units: micromols N per liter per hour (µmol N /L^1/h^1)
Description: <p>Standard deviation of nitrate uptake rate (triplicates)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922550.rdf
Name: NO2_avg_uptake_rate
Units: micromols N per liter per hour (µmol N /L^1/h^1)
Description: <p>Nitrite average uptake rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/922551.rdf
Name: SD_NO2
Units: micromols N per liter per hour (µmol N /L^1/h^1)
Description: <p>Standard deviation of nitrite uptake rate (triplicates)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922552.rdf
Name: Urea_avg_uptake_rate
Units: micromols N per liter per hour (µmol N /L^1/h^1)
Description: <p>Urea average uptake rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/922553.rdf
Name: SD_Urea
Units: micromols N per liter per hour (µmol N /L^1/h^1)
Description: <p>Standard deviation of urea uptake rate (triplicates)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922554.rdf
Name: AAs_avg_uptake_rate
Units: micromols N per liter per hour (µmol N /L^1/h^1)
Description: <p>Amino acids average uptake rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/922555.rdf
Name: SD_AAs
Units: micromols N per liter per hour (µmol N /L^1/h^1)
Description: <p>Standard deviation of amino acids uptake rate (triplicates)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922556.rdf
Name: Cyanate_avg_uptake_rate
Units: micromols N per liter per hour (µmol N /L^1/h^1)
Description: <p>Cyanate average uptake rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/922557.rdf
Name: SD_cyanate
Units: micromols N per liter per hour (µmol N /L^1/h^1)
Description: <p>Standard deviation of cyanate uptake rate (triplicates)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922558.rdf
Name: Specific_NH4_uptake_rate
Units: per hour (h^-1)
Description: <p>Specific ammonium value independent from partiulate nitrogen concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/922559.rdf
Name: Specific_NO3_uptake_rate
Units: per hour (h^-1)
Description: <p>Specific nitrate uptake rate value independent from particulate nitrogen concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/922560.rdf
Name: Specific_NO2_uptake_rate
Units: per hour (h^-1)
Description: <p>Specific nitrite value independent from particulate nitrogen concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/922561.rdf
Name: Specific_urea_uptake_rate
Units: per hour (h^-1)
Description: <p>Specific urea uptake rate value independent from particulate nitrogen concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/922562.rdf
Name: Specific_AAs_uptake_rate
Units: per hour (h^-1)
Description: <p>Specific amino acid uptake rate independent from particulate nitrogen concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/922563.rdf
Name: Specific_cyanate_uptake_rate
Units: per hour (h^-1)
Description: <p>Specific cyanate uptake rate independent from particulate nitrogen contentration</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
26637
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/ef7598b7-4357-460d-9539-a921e65a3698/content
download
https://doi.org/10.26008/1912/bco-dmo.920423.1
download
onLine
dataset
<p><strong>Particulate Carbon and Nitrogen (PNPC)</strong></p>
<p>Whole water from three target depths (near surface, the depth above the Chl maximum (or near the bottom of the mixed layer), and the depth of the Chl maximum) at each station was collected from Niskin bottles into 10 L carboys and transported to the on-board laboratory where water samples were mixed and sub-samples (0.2–1.4 L) were collected onto pre-combusted GF-75 filters (Whatman<sup>®</sup>, nominal pore size 0.3 μm) in triplicate, for analysis of particulate nitrogen (PN) and carbon (PC) concentrations and the natural abundance of <sup>15</sup>N and <sup>13</sup>C. The filters were stored in cryovials and immediately frozen and stored in a freezer at -20 °C until analysis. Prior to their analysis, filters were dried at 40 ºC, pelletized in tin capsules, and analyzed on a Europa 20/20 isotope ratio mass spectrometer equipped with an automated N and C analyzer. The detection limit of the mass spectrometer was 0.0018 and 0.0005 atom% for <sup>15</sup>N and <sup>13</sup>C, respectively; these values were derived based on three times the standard deviation (3 × SD) of the atom% of 12.5 µg N and 100 µg C standards (n=40).</p>
<p>&nbsp;</p>
<p><strong>Nitrogen Uptake&nbsp;</strong></p>
<p>Parallel sets of triplicate whole water (0.5−2 L) from the three target depths mentioned above (near surface, above the depth of the Chl maximum (or near the bottom of the mixed layer), and at the depth of the Chl maximum) from 27 stations were dispensed from 10 L carboys into acid-cleaned polyethylene terephthalate glycol incubation bottles (Nalgene<sup>TM</sup>).</p>
<p>Nitrogen uptake incubations were initiated by amending incubation bottles with highly enriched (98−99%) <sup>15</sup>N-labeled substrates (Cambridge Isotope Laboratories, Inc., USA), including ammonium chloride (<sup>15</sup>NH<sub>4</sub>Cl), potassium nitrate (K<sup>15</sup>NO<sub>3</sub>), potassium nitrite (K<sup>15</sup>NO<sub>2</sub>), and <sup>15</sup>N- and <sup>13</sup>C-labeled potassium cyanate (KO<sup>13</sup>C<sup>15</sup>N), urea (<sup>13</sup>CO(<sup>15</sup>NH<sub>2</sub>)<sub>2</sub>), and algal amino acid mixture.</p>
Specified by the Principal Investigator(s)
<p>Nitrogen update rates were calculated via the formula included in the supplemental files section of this metadata landing page.&nbsp;<img alt="" 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" /></p>
<p>Within this formula,<em> (atom% PN)initial</em>&nbsp;and <em>(atom% PN)final&nbsp;</em>represent the 15N isotopic composition of the particulate pool at the initial and final time points of the incubation period; <em>atom% N source pool</em>&nbsp;is the 15N isotopic enrichment of the dissolved nitrogen pool after the tracer addition; [PN] represents the concentration of the particulate nitrogen pool; here, we used the average value between the initial and final PN concentrations for this calculation.</p>
Specified by the Principal Investigator(s)
- Converted date values from %m/%d/%Y format to %Y-%m-%d format
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PI Supplied Instrument Name: PI Supplied Instrument Description:An isotope ratio mass spectrometer was used for PNPC measurement. Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
10 L Niskin bottles
10 L Niskin bottles
PI Supplied Instrument Name: 10 L Niskin bottles PI Supplied Instrument Description:Dissolved seawater samples were collected using a sampling rosette equipped with a 12 X 10 L Niskin bottles. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: HRS1610
HRS1610
R/V Hugh R. Sharp
Community Standard Description
International Council for the Exploration of the Sea
R/V Hugh R. Sharp
vessel
HRS1610
Margaret Mulholland
Old Dominion University
R/V Hugh R. Sharp
Community Standard Description
International Council for the Exploration of the Sea
R/V Hugh R. Sharp
vessel