http://lod.bco-dmo.org/id/dataset/920812
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-03-01
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Fertilization rate of gametes following parental ocean acidification during lab experiments conducted in spring 2022.
2024-03-24
publication
2024-03-24
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-03-24
publication
https://doi.org/10.26008/1912/bco-dmo.920812.1
Katie Barott
University of Pennsylvania
principalInvestigator
Kristen Brown
University of Pennsylvania
principalInvestigator
Kelsey Speer
University of Pennsylvania
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Glass, B., Brown, K., Speer, K., Barott, K., Schmitt, A. (2024) Fertilization rate of gametes following parental ocean acidification during lab experiments conducted in spring 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-03-24 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.920812.1 [access date]
OA experiment: Nematostella fertilization Dataset Description: <p>Data generated as part of a Nematostella ocean acidfication experiment published in Glass et al., 2023. (see Related Publications). Related Zenodo datasets provides further analysis and plotting of the BCO-DMO dataset here. (see Related Dataset).</p> Methods and Sampling: <div class="fieldset-wrapper">
<div class="field field-name-field-dataset-acquisition-descri field-type-text-long field-label-hidden">
<div class="field-items">
<div class="field-item even">
<p><em>Nematostella vectensis</em>&nbsp;(Stephenson, 1935) anemones were collected from a salt marsh in Brigantine, New Jersey in the fall of 2020. Females were identified by inducing spawning, and 14 individuals that released eggs were chosen as the genotype pool for this experiment. Each female was then horizontally bisected through the body column using a razor blade, resulting in two genotypically identical individuals that were divided between the two experimental groups (ambient and acidic).</p>
<p>A clonal male population, also originating from the United States Atlantic coast, was obtained from the laboratory of Dr. Katerina Ragkousi (Amherst College) in the spring of 2021. The male population size was increased via bisection, resulting in a total of 20 genetically identical males for the experiment (N=10 per treatment).</p>
<p>All anemones were kept in 12 parts per thousand (ppt) artificial seawater (ASW; Instant Ocean Reef Crystals<sup>Ⓡ</sup>&nbsp;reef salt, Spectrum Brands, Blacksburg, VA, USA) at pH 7.7–8.1 and 18°C. The animals were maintained in a dark incubator (Boekel Scientific, Feasterville-Trevose, PA, USA) and fed approximately every other day with Artemia nauplii. The experiment was performed approximately 1–1.5 years after animal collection.</p>
</div>
</div>
</div>
</div>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1923743 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1923743
completed
Katie Barott
University of Pennsylvania
215-746-6911
433 South University Avenue Leidy Laboratories 326
Philadelphia
PA
19104
USA
kbarott@sas.upenn.edu
pointOfContact
Kristen Brown
University of Pennsylvania
433 S University Ave
Philadelphia
PA
19104-4544
USA
ktbrown@sas.upenn.edu
pointOfContact
Kelsey Speer
University of Pennsylvania
kspeer@sas.upenn.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
Treatment
Sperm_Concentration
Fertilization_Success
theme
None, User defined
time_point
treatment
ratio
featureType
BCO-DMO Standard Parameters
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Influence of environmental pH variability and thermal sensitivity on the resilience of reef-building corals to acidification stress
https://osprey.bco-dmo.org/project/843347
Influence of environmental pH variability and thermal sensitivity on the resilience of reef-building corals to acidification stress
<p><strong>NSF Award Abstract:</strong><br />
Coral reefs are incredibly diverse ecosystems that provide food, tourism revenue, and shoreline protection for coastal communities. The ability of coral reefs to continue providing these services to society is currently threatened by climate change, which has led to increasing ocean temperatures and acidity that can lead to the death of corals, the animals that build the reef framework upon which so many species depend. This project examines how temperature and acidification stress work together to influence the future health and survival of corals. The scientists are carrying out the project in Hawaii where they have found individual corals with different sensitivities to temperature stress that are living on reefs with different environmental pH conditions. This project improves understanding of how an individual coral's history influences its response to multiple stressors and helps identify the conditions that are most likely to support resilient coral communities. The project will generate extensive biological and physicochemical data that will be made freely available. Furthermore, this project supports the education and training of undergraduate and high school students and one postdoctoral researcher in marine science and coral reef ecology. Hands-on activities for high school students are being developed into a free online educational resource.</p>
<p>This project compares coral responses to acidification stress in populations experiencing distinct pH dynamics (high diel variability vs. low diel variability) and with distinct thermal tolerances (historically bleaching sensitive vs. tolerant) to learn about how coral responses to these two factors differ between coral species and within populations. Experiments focus on the two dominant reef builders found at these stable and variable pH reefs: Montipora capitata and Porites compressa. Individuals of each species exhibiting different thermal sensitivities (i.e., bleached vs. pigmented) were tagged during the 2015 global coral bleaching event. This system tests the hypotheses that 1) corals living on reefs with larger diel pH fluctuations have greater resilience to acidification stress, 2) coral resilience to acidification is a plastic trait that can be promoted via acclimatization, and 3) thermally sensitive corals have reduced capacity to cope with pH stress, which is exacerbated at elevated temperatures. Coral cells isolated from colonies from each environmental and bleaching history are exposed to acute pH stress and examined for their ability to recover intracellular pH in vivo using confocal microscopy, and the expression level of proteins predicted to be involved in this recovery (e.g., proton transporters) is examined via Western blot and immunolocalization. Corals from each pH history are exposed to stable and variable seawater pH in a controlled aquarium setting to determine the level of plasticity of acidification resilience and to test for pH acclimatization in this system. Finally, corals with different levels of thermal sensitivity are exposed to thermal stress and recovery, and their ability to regulate pH is examined over time. The results of these experiments help identify reef conditions that promote coral resilience to ocean acidification against the background of increasingly common thermal stress events, while advancing mechanistic understanding of coral physiology and symbiosis.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Coral Resilience
largerWorkCitation
project
eng; USA
oceans
2022-01-26
2022-05-02
Kaneohe Bay, Oahu, HI; Heron Island, Queensland, Australia
0
BCO-DMO catalogue of parameters from Fertilization rate of gametes following parental ocean acidification during lab experiments conducted in spring 2022.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/921078.rdf
Name: Date
Units: unitless
Description: <p>Experimental treatment into which anemones were placed (ambient or acidic seawater pH)</p>
http://lod.bco-dmo.org/id/dataset-parameter/921079.rdf
Name: Treatment
Units: unitless
Description: <p>Sampling week (week 1 start = 2022-01-26)</p>
http://lod.bco-dmo.org/id/dataset-parameter/921080.rdf
Name: Sperm_Concentration
Units: cells/mL
Description: <p>Concentratin of sperm used to fertilize egg bundle</p>
http://lod.bco-dmo.org/id/dataset-parameter/921081.rdf
Name: Fertilization_Success
Units: percentage (%)
Description: <p>Percent of eggs in bundle successfully fertilized</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2417
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/66213bd7-6fb5-481c-a8f7-c1b3bc73ae57/content
download
https://doi.org/10.26008/1912/bco-dmo.920812.1
download
onLine
dataset
<div class="fieldset-wrapper">
<div class="field field-name-field-dataset-acquisition-descri field-type-text-long field-label-hidden">
<div class="field-items">
<div class="field-item even">
<p><em>Nematostella vectensis</em>&nbsp;(Stephenson, 1935) anemones were collected from a salt marsh in Brigantine, New Jersey in the fall of 2020. Females were identified by inducing spawning, and 14 individuals that released eggs were chosen as the genotype pool for this experiment. Each female was then horizontally bisected through the body column using a razor blade, resulting in two genotypically identical individuals that were divided between the two experimental groups (ambient and acidic).</p>
<p>A clonal male population, also originating from the United States Atlantic coast, was obtained from the laboratory of Dr. Katerina Ragkousi (Amherst College) in the spring of 2021. The male population size was increased via bisection, resulting in a total of 20 genetically identical males for the experiment (N=10 per treatment).</p>
<p>All anemones were kept in 12 parts per thousand (ppt) artificial seawater (ASW; Instant Ocean Reef Crystals<sup>Ⓡ</sup>&nbsp;reef salt, Spectrum Brands, Blacksburg, VA, USA) at pH 7.7–8.1 and 18°C. The animals were maintained in a dark incubator (Boekel Scientific, Feasterville-Trevose, PA, USA) and fed approximately every other day with Artemia nauplii. The experiment was performed approximately 1–1.5 years after animal collection.</p>
</div>
</div>
</div>
</div>
Specified by the Principal Investigator(s)
<p>Fertilization rates were quantified in weeks 2, 4, and 9. Sperm water was combined into a single pool per treatment and each pool was diluted to the same concentration, which differed between spawning dates. Each pool was then serially diluted 1:2 in 12 ppt ASW to obtain four different sperm concentrations. Egg bundles were separated into six-well plates, with 1–2 bundles per well, and 6 mL of sperm water from the corresponding experimental treatment was pipetted into each well, with three replicates per sperm concentration per treatment for a total of 12 wells per pH treatment. At 3 h post-fertilization (HPF), each well was photographed using a dissecting microscope with a camera attachment, and the number of fertilized and unfertilized eggs were counted manually. Fertilized eggs were identified by their conspicuous bumpy appearance, which is a result of initial cell divisions, whereas unfertilized eggs maintained a round shape. For each well, the percentage of fertilized eggs was calculated by dividing the number of fertilized eggs by the total number of eggs in the well.</p>
Specified by the Principal Investigator(s)
* Adjusted parameter names to comply with database requirements
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact