http://lod.bco-dmo.org/id/dataset/921847
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-03-11
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
McLane pumps log deployed on the R/V Atlantis CliOMZ AT50-10 expedition from Golfito Costa Rica to San Diego USA that occurred in May - June of 2023.
2024-04-30
publication
2024-04-30
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-04-30
publication
https://doi.org/10.26008/1912/bco-dmo.921847.1
Mak A. Saito
Woods Hole Oceanographic Institution
principalInvestigator
Alyson E. Santoro
University of California-Santa Barbara
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Saito, M. A., Lopez, P. Z., Santoro, A. E. (2024) McLane pumps log deployed on the R/V Atlantis CliOMZ AT50-10 expedition from Golfito Costa Rica to San Diego USA that occurred in May - June of 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-04-30 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.921847.1 [access date]
McLane pump logs - AUV Clio cruise Dataset Description: Methods and Sampling: <p>Seven Large Volume Water Transfer Systems (McLane pumps) were deployed during research cruise AT50-10 attached to a synthetic wire using a&nbsp;MASH2K&nbsp;winch and a dual filter sampler system. Deployments lasted 3 or 4 hours and seawater was filtered at specific depths. A combination of 0.2 um Supor membranes&nbsp;and 51 um pore size Nitex mesh was used for one side while the other side pump water through a Glass Fiber Filter (GFF). See data log for specific size fraction combination as well as specific samples taken.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1924554 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1924554
completed
Mak A. Saito
Woods Hole Oceanographic Institution
508-289-2393
266 Woods Hole Rd. MS #51 Watson Lab
Woods Hole
MA
02543
USA
msaito@whoi.edu
pointOfContact
Alyson E. Santoro
University of California-Santa Barbara
805-893-5318
2155 Marine Biotech Lab
Santa Barbara
CA
93106
USA
asantoro@ucsb.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cruise_ID
Start_date_UTC
Start_time_UTC
End_date_UTC
End_time_UTC
Station_number
Start_Lat
Start_Long
End_Lat
End_Long
Cast_number
Pump_number
Head_number_GFF_port
Head_number_Supor_port
Winch_payout_reading
Corrected_winch_payout
Depth
Total_time_filtered
GFF_side_flow_meter_volume
Supor_side_flow_meter_volume
Filter_diameter
Comments_deployment
SAMPLE_PRFEIX
SUPOR_0_2_FRACTION_PROTEIN1
SUPOR_0_2_FRACTION_DNA1
SUPOR_0_2_FRACTION_DNA2
SUPOR_0_2 FRACTION_eDNA
SUPOR_0_2_FRACTION_pMetal
SUPOR_0_2 FRACTION_POS
SUPOR_0_2_FRACTION_metatranscriptomics_Intercomparison
SUPOR_0_2_FRACTION_metagenomics_Intercomparison
NITEX_51_FRACTION_PROTEIN1
NITEX_51_FRACTION_DNA1
NITEX_51_FRACTION_pMetal
NITEX_51_FRACTION_CULTURING
GFF_FRACTION_PROTEIN1
GFF_FRACTION_CA
GFF_FRACTION_PIGS
GFF_FRACTION_POC
GFF_FRACTION_HG
SAMPLE_COMMENT
Large Volume Water Transfer Systems (McLane pumps)
theme
None, User defined
Cruise identfier
start_date
time begin
end_date
time_end
station
latitude at start time of measurement
longitude at start time of measurement
latitude at end time of measurement
longitude at end time of measurement
cast
sample identification
wire out
depth
sample time
volume of water filtered
diameter
comments
featureType
BCO-DMO Standard Parameters
McLane Large Volume Pumping System WTS-LV
instrument
BCO-DMO Standard Instruments
AT50-10
service
Deployment Activity
Eastern Tropical and Subtropical Pacific Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Underexplored Connections between Nitrogen and Trace Metal Cycling in Oxygen Minimum Zones Mediated by Metalloenzyme Inventories
https://osprey.bco-dmo.org/project/806565
Collaborative Research: Underexplored Connections between Nitrogen and Trace Metal Cycling in Oxygen Minimum Zones Mediated by Metalloenzyme Inventories
<p><em>NSF abstract:</em><br />
Though scarce and largely insoluble, trace metals are key components of sophisticated enzymes (protein molecules that speed up biochemical reactions) involved in biogeochemical cycles in the dark ocean (below 1000m). For example, metalloenzymes are involved in nearly every reaction in the nitrogen cycle. Yet, despite direct connections between trace metal and nitrogen cycles, the relationship between trace metal distributions and biological nitrogen cycling processes in the dark ocean have rarely been explored, likely due to the technical challenges associated with their study. Availability of the autonomous underwater vehicle (AUV) Clio, a sampling platform capable of collecting high-resolution vertical profile samples for biochemical and microbial measurements by large volume filtration of microbial particulate material, has overcome this challenge. Thus, this research project plans an interdisciplinary chemistry, biology, and engineering effort to test the hypothesis that certain chemical reactions, such as nitrite oxidation, could become limited by metal availability within the upper mesopelagic and that trace metal demands for nitrite-oxidizing bacteria may be increased under low oxygen conditions. Broader impacts of this study include the continued development and application of the Clio Biogeochemical AUV as a community resource by developing and testing its high-resolution and adaptive sampling capabilities. In addition, metaproteomic data will be deposited into the recently launched Ocean Protein Portal to allow oceanographers and the metals in biology community to examine the distribution of proteins and metalloenzymes in the ocean. Undergraduate students will be supported by this project at all three institutions, with an effort to recruit minority students. The proposed research will also be synergistic with the goals of early community-building efforts for a potential global scale microbial biogeochemistry program modeled after the success of the GEOTRACES program, provisionally called "Biogeoscapes: Ocean metabolism and nutrient cycles on a changing planet".</p>
<p>The proposed research project will test the following three hypotheses: (1) the microbial metalloenzyme distribution of the mesopelagic is spatially dynamic in response to environmental gradients in oxygen and trace metals, (2) nitrite oxidation in the Eastern Tropical Pacific Ocean can be limited by iron availability in the upper mesopelagic through an inability to complete biosynthesis of the microbial protein nitrite oxidoreductase, and (3) nitrite-oxidizing bacteria increase their metalloenzyme requirements at low oxygen, impacting the distribution of both dissolved and particulate metals within oxygen minimum zones. One of the challenges to characterizing the biogeochemistry of the mesopelagic ocean is an inability to effectively sample it. As a sampling platform, we will use the novel biogeochemical AUV Clio that enables high-resolution vertical profile samples for biochemical and microbial measurements by large volume filtration of microbial particulate material on a research expedition in the Eastern Tropical Pacific Ocean. Specific research activities will be orchestrated to test the hypotheses. Hypothesis 1 will be explored by comparison of hydrographic, microbial distributions, dissolved and particulate metal data, and metaproteomic results with profile samples collected by Clio. Hypothesis 2 will be tested by incubation experiments using 15NO2- oxidation rates on Clio-collected incubation samples. Hypothesis 3 will be tested by dividing targeted nitrite oxidoreductase protein copies by qPCR (quantitative polymerase chain reaction)-based nitrite oxidizing bacteria abundance (NOB) to determine if cellular copy number varies with oxygen distributions, and by metalloproteomic analyses of NOB cultures. The demonstration of trace metal limitation of remineralization processes, not just primary production, would transform our understanding of the role of metals in biogeochemical cycling and provide new ways with which to interpret sectional data of dissolved and particulate trace metal distributions in the ocean. The idea that oxygen may play a previously underappreciated role in controlling trace metals due not just to metals' physical chemistry, but also from changing biological demand, will improve our ability to predict trace metal distributions in the face of decreasing ocean oxygen content.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
CliOMZ
largerWorkCitation
project
eng; USA
oceans
Eastern Tropical and Subtropical Pacific Ocean
-118.098
-88.9709
-10.0244
25.0802
2023-05-04
2023-06-06
Eastern Tropical Pacific
0
BCO-DMO catalogue of parameters from McLane pumps log deployed on the R/V Atlantis CliOMZ AT50-10 expedition from Golfito Costa Rica to San Diego USA that occurred in May - June of 2023.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/922471.rdf
Name: Cruise_ID
Units: unitless
Description: <p>Cruise ID AT50-10</p>
http://lod.bco-dmo.org/id/dataset-parameter/922472.rdf
Name: Start_date_UTC
Units: unitless
Description: <p>Pump start date</p>
http://lod.bco-dmo.org/id/dataset-parameter/922473.rdf
Name: Start_time_UTC
Units: unitless
Description: <p>Pump start time</p>
http://lod.bco-dmo.org/id/dataset-parameter/922474.rdf
Name: End_date_UTC
Units: unitless
Description: <p>Pump end date</p>
http://lod.bco-dmo.org/id/dataset-parameter/922475.rdf
Name: End_time_UTC
Units: unitless
Description: <p>Pump end time</p>
http://lod.bco-dmo.org/id/dataset-parameter/922476.rdf
Name: Station_number
Units: unitless
Description: <p>Station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/922477.rdf
Name: Start_Lat
Units: decimal degrees
Description: <p>Pump start latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/922478.rdf
Name: Start_Long
Units: decimal degrees
Description: <p>Pump start longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/922479.rdf
Name: End_Lat
Units: decimal degrees
Description: <p>Pump end latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/922480.rdf
Name: End_Long
Units: decimal degrees
Description: <p>Pump end longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/922481.rdf
Name: Cast_number
Units: unitless
Description: <p>Cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/922482.rdf
Name: Pump_number
Units: unitless
Description: <p>Pump number</p>
http://lod.bco-dmo.org/id/dataset-parameter/922483.rdf
Name: Head_number_GFF_port
Units: unitless
Description: <p>Pump head identification number at GFF water port in dual samlpler system</p>
http://lod.bco-dmo.org/id/dataset-parameter/922484.rdf
Name: Head_number_Supor_port
Units: unitless
Description: <p>Pump head identification number at Supor/Nitex water port in dual samlpler system</p>
http://lod.bco-dmo.org/id/dataset-parameter/922485.rdf
Name: Winch_payout_reading
Units: meters (m)
Description: <p>Winch payout reading</p>
http://lod.bco-dmo.org/id/dataset-parameter/922486.rdf
Name: Corrected_winch_payout
Units: meters (m)
Description: <p>Correct winch payout</p>
http://lod.bco-dmo.org/id/dataset-parameter/922487.rdf
Name: Depth
Units: meters (m)
Description: <p>Sample depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/922488.rdf
Name: Total_time_filtered
Units: minutes (min)
Description: <p>Total time filtered</p>
http://lod.bco-dmo.org/id/dataset-parameter/922489.rdf
Name: GFF_side_flow_meter_volume
Units: liters (L)
Description: <p>Volume of water filtered through the GFF side in dual sampler system</p>
http://lod.bco-dmo.org/id/dataset-parameter/922490.rdf
Name: Supor_side_flow_meter_volume
Units: liters (L)
Description: <p>Volume of water filtered through the Supor side in dual sampler system</p>
http://lod.bco-dmo.org/id/dataset-parameter/922491.rdf
Name: Filter_diameter
Units: millimeters (mm)
Description: <p>Filter diameter</p>
http://lod.bco-dmo.org/id/dataset-parameter/922492.rdf
Name: Comments_deployment
Units: unitless
Description: <p>Comments on deployment</p>
http://lod.bco-dmo.org/id/dataset-parameter/922493.rdf
Name: SAMPLE_PRFEIX
Units: unitless
Description: <p>Sample prefix</p>
http://lod.bco-dmo.org/id/dataset-parameter/922494.rdf
Name: SUPOR_0_2_FRACTION_PROTEIN1
Units: unitless
Description: <p>Fraction of 142mm diameter 0.2um pore size Supor (PES) filter assigned for global metaproteomic analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/922495.rdf
Name: SUPOR_0_2_FRACTION_DNA1
Units: unitless
Description: <p>Fraction of 142mm diameter 0.2um pore size Supor (PES) filter assigned for genomic DNA analysis, sample 1 of 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/922496.rdf
Name: SUPOR_0_2_FRACTION_DNA2
Units: unitless
Description: <p>Fraction of 142mm diameter 0.2um pore size Supor (PES) filter assigned for genomic DNA analysis, sample 2 of 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/922497.rdf
Name: SUPOR_0_2 FRACTION_eDNA
Units: unitless
Description: <p>Fraction of 142mm diameter 0.2um pore size Supor (PES) filter assigned for environmental DNA analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/922498.rdf
Name: SUPOR_0_2_FRACTION_pMetal
Units: unitless
Description: <p>Fraction of 142mm diameter 0.2um pore size Supor (PES) filter assigned for particulate metal content analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/922499.rdf
Name: SUPOR_0_2 FRACTION_POS
Units: unitless
Description: <p>Fraction of 142mm diameter 0.2um pore size Supor (PES) filter assigned for particulate organic sulfur analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/922500.rdf
Name: SUPOR_0_2_FRACTION_metatranscriptomics_Intercomparison
Units: unitless
Description: <p>Fraction of 142mm diameter 0.2um pore size Supor (PES) filter assigned for metatranscriptomic intercomparison analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/922501.rdf
Name: SUPOR_0_2_FRACTION_metagenomics_Intercomparison
Units: unitless
Description: <p>Fraction of 142mm diameter 0.2um pore size Supor (PES) filter assigned for metagenomic intercomparison analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/922502.rdf
Name: NITEX_51_FRACTION_PROTEIN1
Units: unitless
Description: <p>Fraction of 142mm diameter 51um pore size Nitex filter assigned for global metaproteomics</p>
http://lod.bco-dmo.org/id/dataset-parameter/922503.rdf
Name: NITEX_51_FRACTION_DNA1
Units: unitless
Description: <p>Fraction of 142mm diameter 51um pore size Nitex filter assigned for genomic DNA analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/922504.rdf
Name: NITEX_51_FRACTION_pMetal
Units: unitless
Description: <p>Fraction of 142mm diameter 51um pore size Nitex filter assigned for particulate metal content analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/922505.rdf
Name: NITEX_51_FRACTION_CULTURING
Units: unitless
Description: <p>Fraction of 142mm diameter 51um pore size Nitex filter assigned for bacteria culturing</p>
http://lod.bco-dmo.org/id/dataset-parameter/922506.rdf
Name: GFF_FRACTION_PROTEIN1
Units: unitless
Description: <p>Fraction of 142mm diameter 0.7um pore size Glass Fiber Filter (GFF) filter assigned for global metaproteomics</p>
http://lod.bco-dmo.org/id/dataset-parameter/922507.rdf
Name: GFF_FRACTION_CA
Units: unitless
Description: <p>Fraction of 142mm diameter 0.7um pore size Glass Fiber Filter (GFF) filter assigned for carbonic anhydrase activity analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/922508.rdf
Name: GFF_FRACTION_PIGS
Units: unitless
Description: <p>Fraction of 142mm diameter 0.7um pore size Glass Fiber Filter (GFF) filter assigned for photosynthetic pigments analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/922509.rdf
Name: GFF_FRACTION_POC
Units: unitless
Description: <p>Fraction of 142mm diameter 0.7um pore size Glass Fiber Filter (GFF) filter assigned for particulate organic carbon analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/922510.rdf
Name: GFF_FRACTION_HG
Units: unitless
Description: <p>Fraction of 142mm diameter 0.7um pore size Glass Fiber Filter (GFF) filter assigned for mercury content analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/922511.rdf
Name: SAMPLE_COMMENT
Units: unitless
Description: <p>Comments on sample taken</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
44414
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/b4e1191a-a24d-423a-baae-ecf449f75f6a/content
download
https://doi.org/10.26008/1912/bco-dmo.921847.1
download
onLine
dataset
<p>Seven Large Volume Water Transfer Systems (McLane pumps) were deployed during research cruise AT50-10 attached to a synthetic wire using a&nbsp;MASH2K&nbsp;winch and a dual filter sampler system. Deployments lasted 3 or 4 hours and seawater was filtered at specific depths. A combination of 0.2 um Supor membranes&nbsp;and 51 um pore size Nitex mesh was used for one side while the other side pump water through a Glass Fiber Filter (GFF). See data log for specific size fraction combination as well as specific samples taken.</p>
Specified by the Principal Investigator(s)
<p>The depths for McLane pumps&nbsp;(which lack pressure sensors)&nbsp;were calculated based on winch payout and a calibration curve generated aboard ship. The need for calibration arises from the MASH2K winch not being used with metallic wire for this application. By using (non-conducting) synthetic wire for trace metal clean needs, the wire wraps are not predictably uniform as with metallic wire. As a result and the MASH2K instrument borrowed from the East Coast Winch Pool was not programmed for depths using synthetic wire. To compensate for this the McLane pump payout calibration was estimated using the Trace Metal rosette deployed with a SSSG Pinger, with depth from the ship and the winch payout recorded at 17 intervals between 0 and 4287 meters. A power law relationship was calculated from this relationship by Tristan Horner where: True Payout = Raw Payout / ( 1.34 x Raw Payout^-0.026 ). This equation had a mean absolute standard deviation of 5m for the 17 depths recorded, with depth variance increasing with depth. This equation was applied to the payout readings from the winch when used for McLane pumps to calculate the deployment Depth (m) parameter.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Large Volume Water Transfer Systems (McLane pumps)
Large Volume Water Transfer Systems (McLane pumps)
PI Supplied Instrument Name: Large Volume Water Transfer Systems (McLane pumps) PI Supplied Instrument Description:Seven Large Volume Water Transfer Systems (McLane pumps; McLane labs: https://mclanelabs.com/wts-lv-large-volume-pump/) were deployed during research cruise AT50-10 attached to a synthetic wire using a MASH2K winch and a dual filter sampler system. A combination of 0.2 um Supor membranes and 51 um pore size Nitex mesh was used for one side while the other side pump water through a Glass Fiber Filter (GFF). Instrument Name: McLane Large Volume Pumping System WTS-LV Instrument Short Name:WTS-LV Instrument Description: The WTS-LV is a Water Transfer System (WTS) Large Volume (LV) pumping instrument designed and manufactured by McLane Research Labs (Falmouth, MA, USA). It is a large-volume, single-event sampler that collects suspended and dissolved particulate samples in situ.
Ambient water is drawn through a modular filter holder onto a 142-millimeter (mm) membrane without passing through the pump. The standard two-tier filter holder provides prefiltering and size fractioning. Collection targets include chlorophyll maximum, particulate trace metals, and phytoplankton. It features different flow rates and filter porosity to support a range of specimen collection. Sampling can be programmed to start at a scheduled time or begin with a countdown delay. It also features a dynamic pump speed algorithm that adjusts flow to protect the sample as material accumulates on the filter. Several pump options range from 0.5 to 30 liters per minute, with a max volume of 2,500 to 36,000 liters depending on the pump and battery pack used. The standard model is depth rated to 5,500 meters, with a deeper 7,000-meter option available. The operating temperature is -4 to 35 degrees Celsius.
The WTS-LV is available in four different configurations: Standard, Upright, Bore Hole, and Dual Filter Sampler. The high-capacity upright WTS-LV model provides three times the battery life of the standard model. The Bore-Hole WTS-LV is designed to fit through a narrow opening such as a 30-centimeter borehole. The dual filter WTS-LV features two vertical intake 142 mm filter holders to allow simultaneous filtering using two different porosities.
Cruise: AT50-10
AT50-10
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT50-10
Mak A. Saito
Woods Hole Oceanographic Institution
https://www.rvdata.us/search/cruise/AT50-10
Report describing AT50-10
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel