http://lod.bco-dmo.org/id/dataset/922037 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2024-03-12 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) pH and total alkalinity measurements of treatment tub seawater during lab experiments conducted in spring 2022. 2024-03-24 publication 2024-03-24 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2024-03-24 publication https://doi.org/10.26008/1912/bco-dmo.922037.1 Katie Barott University of Pennsylvania principalInvestigator Kristen Brown University of Pennsylvania principalInvestigator Kelsey Speer University of Pennsylvania principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Glass, B., Brown, K., Speer, K., Barott, K., Schmitt, A. (2024) pH and total alkalinity measurements of treatment tub seawater during lab experiments conducted in spring 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-03-24 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.922037.1 [access date] OA experiment: Nematostella adult respiration Dataset Description: &lt;p&gt;Data generated as part of a Nematostella ocean acidfication experiment published in Glass et al., 2023. (see Related Publications). Related Zenodo datasets provides further analysis and plotting of the BCO-DMO dataset here. (see Related Dataset).&lt;/p&gt; Methods and Sampling: &lt;p&gt;&lt;em&gt;Nematostella vectensis&lt;/em&gt;&amp;nbsp;(Stephenson, 1935) anemones were collected from a salt marsh in Brigantine, New Jersey in the fall of 2020. Females were identified by inducing spawning, and 14 individuals that released eggs were chosen as the genotype pool for this experiment. Each female was then horizontally bisected through the body column using a razor blade, resulting in two genotypically identical individuals that were divided between the two experimental groups (ambient and acidic).&lt;/p&gt; &lt;p&gt;A clonal male population, also originating from the United States Atlantic coast, was obtained from the laboratory of Dr. Katerina Ragkousi (Amherst College) in the spring of 2021. The male population size was increased via bisection, resulting in a total of 20 genetically identical males for the experiment (N=10 per treatment).&lt;/p&gt; &lt;p&gt;All anemones were kept in 12 parts per thousand (ppt) artificial seawater (ASW; Instant Ocean Reef Crystals&lt;sup&gt;Ⓡ&lt;/sup&gt;&amp;nbsp;reef salt, Spectrum Brands, Blacksburg, VA, USA) at pH 7.7–8.1 and 18°C. The animals were maintained in a dark incubator (Boekel Scientific, Feasterville-Trevose, PA, USA) and fed approximately every other day with Artemia nauplii. The experiment was performed approximately 1–1.5 years after animal collection.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1923743 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1923743 completed Katie Barott University of Pennsylvania 215-746-6911 433 South University Avenue Leidy Laboratories 326 Philadelphia PA 19104 USA kbarott@sas.upenn.edu pointOfContact Kristen Brown University of Pennsylvania 433 S University Ave Philadelphia PA 19104-4544 USA ktbrown@sas.upenn.edu pointOfContact Kelsey Speer University of Pennsylvania kspeer@sas.upenn.edu pointOfContact asNeeded Dataset Version: 1 Unknown Date Treatment pH mV Salinity Temperature TA_1 TA_2 TA_average TA Alkalinity Metrohm 905 Titrando (Metrohm, Herisau, Switzerland) Handheld pH glass electrode (Mettler Toledo, Columbus, OH, USA) Handheld meter (YSI Incorporated, Yellow Springs, OH, USA theme None, User defined date treatment pH conductivity salinity temperature of sample or a generic measurement (not in-situ water temp) total alkalinity (TA) featureType BCO-DMO Standard Parameters Metrohm 905 Titrando potentiometric titrator pH Sensor Water Quality Multiprobe instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Influence of environmental pH variability and thermal sensitivity on the resilience of reef-building corals to acidification stress https://osprey.bco-dmo.org/project/843347 Influence of environmental pH variability and thermal sensitivity on the resilience of reef-building corals to acidification stress <p><strong>NSF Award Abstract:</strong><br /> Coral reefs are incredibly diverse ecosystems that provide food, tourism revenue, and shoreline protection for coastal communities. The ability of coral reefs to continue providing these services to society is currently threatened by climate change, which has led to increasing ocean temperatures and acidity that can lead to the death of corals, the animals that build the reef framework upon which so many species depend. This project examines how temperature and acidification stress work together to influence the future health and survival of corals. The scientists are carrying out the project in Hawaii where they have found individual corals with different sensitivities to temperature stress that are living on reefs with different environmental pH conditions. This project improves understanding of how an individual coral's history influences its response to multiple stressors and helps identify the conditions that are most likely to support resilient coral communities. The project will generate extensive biological and physicochemical data that will be made freely available. Furthermore, this project supports the education and training of undergraduate and high school students and one postdoctoral researcher in marine science and coral reef ecology. Hands-on activities for high school students are being developed into a free online educational resource.</p> <p>This project compares coral responses to acidification stress in populations experiencing distinct pH dynamics (high diel variability vs. low diel variability) and with distinct thermal tolerances (historically bleaching sensitive vs. tolerant) to learn about how coral responses to these two factors differ between coral species and within populations. Experiments focus on the two dominant reef builders found at these stable and variable pH reefs: Montipora capitata and Porites compressa. Individuals of each species exhibiting different thermal sensitivities (i.e., bleached vs. pigmented) were tagged during the 2015 global coral bleaching event. This system tests the hypotheses that 1) corals living on reefs with larger diel pH fluctuations have greater resilience to acidification stress, 2) coral resilience to acidification is a plastic trait that can be promoted via acclimatization, and 3) thermally sensitive corals have reduced capacity to cope with pH stress, which is exacerbated at elevated temperatures. Coral cells isolated from colonies from each environmental and bleaching history are exposed to acute pH stress and examined for their ability to recover intracellular pH in vivo using confocal microscopy, and the expression level of proteins predicted to be involved in this recovery (e.g., proton transporters) is examined via Western blot and immunolocalization. Corals from each pH history are exposed to stable and variable seawater pH in a controlled aquarium setting to determine the level of plasticity of acidification resilience and to test for pH acclimatization in this system. Finally, corals with different levels of thermal sensitivity are exposed to thermal stress and recovery, and their ability to regulate pH is examined over time. The results of these experiments help identify reef conditions that promote coral resilience to ocean acidification against the background of increasingly common thermal stress events, while advancing mechanistic understanding of coral physiology and symbiosis.</p> <p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> Coral Resilience largerWorkCitation project eng; USA oceans 2022-01-26 2022-05-02 Kaneohe Bay, Oahu, HI; Heron Island, Queensland, Australia 0 BCO-DMO catalogue of parameters from pH and total alkalinity measurements of treatment tub seawater during lab experiments conducted in spring 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/922080.rdf Name: Date Units: unitless Description: <p>Date on which seawater samples were collected from experimental tubs</p> http://lod.bco-dmo.org/id/dataset-parameter/922081.rdf Name: Treatment Units: unitless Description: <p>Experimental treatment into which anemones were placed (ambient or acidic seawater pH)</p> http://lod.bco-dmo.org/id/dataset-parameter/922082.rdf Name: pH Units: Arbitrary on pH scale Description: <p>Seawater pH</p> http://lod.bco-dmo.org/id/dataset-parameter/922083.rdf Name: mV Units: millivolts (mV) Description: <p>Conductivity of seawater in millivolts</p> http://lod.bco-dmo.org/id/dataset-parameter/922084.rdf Name: Salinity Units: unitless Description: <p>Seawater salinity</p> http://lod.bco-dmo.org/id/dataset-parameter/922085.rdf Name: Temperature Units: Degrees Celsius Description: <p>Temperature of seawater</p> http://lod.bco-dmo.org/id/dataset-parameter/922086.rdf Name: TA_1 Units: umol/kg Description: <p>Total alkalinity of sample replicate 1</p> http://lod.bco-dmo.org/id/dataset-parameter/922087.rdf Name: TA_2 Units: umol/kg Description: <p>Total alkalinity of sample replicate 2</p> http://lod.bco-dmo.org/id/dataset-parameter/922088.rdf Name: TA_average Units: umol/kg Description: <p>Average total alkalinity of sample replicates</p> http://lod.bco-dmo.org/id/dataset-parameter/922089.rdf Name: TA Units: mol/kg Description: <p>Conversion of TA average into mol/kg</p> http://lod.bco-dmo.org/id/dataset-parameter/922090.rdf Name: Alkalinity Units: mol/kg*10^-3 Description: <p>Conversion of TA mol/kg</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 6635 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/ca15260c-86bc-4b38-8c5c-fcf3c267495f/content download https://doi.org/10.26008/1912/bco-dmo.922037.1 download onLine dataset &lt;p&gt;&lt;em&gt;Nematostella vectensis&lt;/em&gt;&amp;nbsp;(Stephenson, 1935) anemones were collected from a salt marsh in Brigantine, New Jersey in the fall of 2020. Females were identified by inducing spawning, and 14 individuals that released eggs were chosen as the genotype pool for this experiment. Each female was then horizontally bisected through the body column using a razor blade, resulting in two genotypically identical individuals that were divided between the two experimental groups (ambient and acidic).&lt;/p&gt; &lt;p&gt;A clonal male population, also originating from the United States Atlantic coast, was obtained from the laboratory of Dr. Katerina Ragkousi (Amherst College) in the spring of 2021. The male population size was increased via bisection, resulting in a total of 20 genetically identical males for the experiment (N=10 per treatment).&lt;/p&gt; &lt;p&gt;All anemones were kept in 12 parts per thousand (ppt) artificial seawater (ASW; Instant Ocean Reef Crystals&lt;sup&gt;Ⓡ&lt;/sup&gt;&amp;nbsp;reef salt, Spectrum Brands, Blacksburg, VA, USA) at pH 7.7–8.1 and 18°C. The animals were maintained in a dark incubator (Boekel Scientific, Feasterville-Trevose, PA, USA) and fed approximately every other day with Artemia nauplii. The experiment was performed approximately 1–1.5 years after animal collection.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;Salinity, pH, and temperature were measured and recorded approximately daily to ensure the maintenance of experimental conditions. Seawater pH was measured and recorded using a handheld pH glass electrode (Mettler Toledo, Columbus, OH, USA), which was calibrated once a week using calibration solutions (pH 7 and 10) supplied by the probe manufacturer. Salinity and temperature were measured and recorded using a handheld meter (YSI Incorporated, Yellow Springs, OH, USA), and salinity was adjusted with deionized (DI) water as needed.&lt;/p&gt; &lt;p&gt;Duplicate 50 mL seawater samples were collected from each tub every three days, then immediately sterilized with a 0.22 μm syringe filter (Sigma-Aldrich, Burlington, MA, USA) and stored at 4°C in conical tubes (Corning, Corning, NY, USA) until processing for total alkalinity (TA). TA was determined via titration using a Metrohm 905 Titrando (Metrohm, Herisau, Switzerland). Parameters of the carbonate system in the seawater samples [e.g. (carbonate), (bicarbonate), aragonite saturation state] were calculated from temperature, salinity, TA, and pH using the&amp;nbsp;&lt;em&gt;seacarb&lt;/em&gt;&amp;nbsp;package in R.&lt;/p&gt; Specified by the Principal Investigator(s) * Adjusted parameter names to comply with database requirements Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Metrohm 905 Titrando (Metrohm, Herisau, Switzerland) Metrohm 905 Titrando (Metrohm, Herisau, Switzerland) PI Supplied Instrument Name: Metrohm 905 Titrando (Metrohm, Herisau, Switzerland) PI Supplied Instrument Description:Metrohm 905 Titrando (Metrohm, Herisau, Switzerland) for total alkalinity determination Instrument Name: Metrohm 905 Titrando potentiometric titrator Instrument Short Name: Instrument Description: The Metrohm 905 Titrando potentiometric titrator is a modular potentiometric titrator for dynamic, monotonic, and set endpoint titrations. The device includes magnetic stirrers, rod stirrers, and a titration stand. It can be connected to various dosing units which include a buret and are attached to the reagent. Operation is carried out by means of a touch-sensitive display or with high-performance PC software. Temperature is measured by a Pt1000 or NTC. Ranges of the outputs are -13 to 20 pH, -1200 to 1200 mV, and -150 to 250 deg. C (Pt1000) or -5 ro 250 deg. C (NTC). Resolutions of the outputs are 0.001 for pH, 0.1 for mV, 0.1 deg. C for temperature. The measuring interval is of 100 ms. Works in conditions from 5 to 45 deg. C and at a maximum of 80 % relative humidity. Handheld pH glass electrode (Mettler Toledo, Columbus, OH, USA) Handheld pH glass electrode (Mettler Toledo, Columbus, OH, USA) PI Supplied Instrument Name: Handheld pH glass electrode (Mettler Toledo, Columbus, OH, USA) PI Supplied Instrument Description:Handheld pH glass electrode (Mettler Toledo, Columbus, OH, USA), calibrated once a week using calibration solutions (pH 7 and 10) supplied by the probe manufacturer Instrument Name: pH Sensor Instrument Short Name:pH Sensor Instrument Description: An instrument that measures the hydrogen ion activity in solutions. The overall concentration of hydrogen ions is inversely related to its pH.  The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).  Handheld meter (YSI Incorporated, Yellow Springs, OH, USA Handheld meter (YSI Incorporated, Yellow Springs, OH, USA PI Supplied Instrument Name: Handheld meter (YSI Incorporated, Yellow Springs, OH, USA PI Supplied Instrument Description:Handheld meter (YSI Incorporated, Yellow Springs, OH, USA) for salnity and temperature, and salinity was adjusted with deionized (DI) water as needed Instrument Name: Water Quality Multiprobe Instrument Short Name:Water Quality Multiprobe Instrument Description: An instrument which measures multiple water quality parameters based on the sensor configuration.