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&lt;p&gt;Identifying the factors that regulate the structure and function of ecosystems is a fundamental challenge for ecological theory and applied science. This challenge is often framed within the context of Top-Down (TD) versus Bottom-Up (BU) regulation, but the extent to which this framework can predict processes in complex, real-world ecosystems is not fully understood. It is now widely recognized that TD/BU factors do not act in isolation. For example, in many ecosystems, consumers contribute to both TD (via consumption) and BU (via excretion) pathways. Environmental factors, including human-induced change, can further alter the nature of these interactions. Quantifying the strength of TD and BU pathways and the extent to which they regulate the structure and function in highly dynamic ecosystems requires an experimental system that is sufficiently tractable that all its components can be quantified, while still being representative of real ecosystems. To address this challenge, this research project creates a unique ecosystem-scale artificial reef (AR) experiment in Haiti to test how two factors (AR structure, and fishing pressure) alter the strength of independent and interactive TD and BU pathways to regulate the structure and function of real-world reef ecosystems. Over the course of four years, the production of seagrass (surrounding the ARs), coral (transplanted onto the ARs), and fish (in and around the ARs) is being measured, providing a quantitative assessment of ecosystem-level production across the two treatments. Linear and structural equation models are used to measure the independent and interactive strengths TD and BU pathways, and to identify the suite of directional relationships between each trophic level that best predict overall ecosystem production. Harnessing the ability to use ecosystem-scale experiments and quantify production across all trophic levels in a highly complex, real-world system enables an unprecedented test of TD/BU theory.&lt;/p&gt;
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