http://lod.bco-dmo.org/id/dataset/922789
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-03-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Compound specific isotope data of amino acids for abyssal macrofauna, megafauna, sediments, sediment traps, and in situ filtered particles at Station ALOHA off Hawaii and Station M off California from 2019 to 2020
2024-04-29
publication
2024-04-29
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-05-09
publication
https://doi.org/10.26008/1912/bco-dmo.922789.1
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
principalInvestigator
Brian N. Popp
University of Hawaiʻi at Mānoa
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Drazen, J. C., Popp, B. N. (2024) Compound specific isotope data of amino acids for abyssal macrofauna, megafauna, sediments, sediment traps, and in situ filtered particles at Station ALOHA off Hawaii and Station M off California from 2019 to 2020. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-04-29 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.922789.1 [access date]
Compound specific isotope data Dataset Description: Methods and Sampling: <p>Macrofauna and megafauna were collected in May and October 2019 using the HOV Alvin<em> </em>and the ROV Doc Ricketts, respectively, at Station M; and in July 2019, January 2020, and July 2020 using ROV Lu'ukai at Station Aloha. Macrofauna at Station M were collected using HOV-operated Ekman cores (20x20 centimeters (cm)). Macrofauna at Station Aloha were collected using a Brenke epibenthic sled towed across the seafloor due to the considerably lower macrofaunal densities at this oligotrophic site. Megafauna were collected using the submersible vehicle's manipulator arm and/or slurp gun. Upon retrieval to the surface, samples were placed in a cool room (5 degrees Celsius (ºC)) for further processing. Specimens of megafauna were weighed and measured, then they were dissected using a scalpel. All tissue samples were placed in cryovials and frozen in liquid nitrogen, and subsequently stored at -80ºC. Sieved (300 micrometers (um)) macrofauna samples were preserved in 10% buffered formalin. In the laboratory, macrofauna were further sorted by taxon. Samples of megafauna body tissues or macrofauna were freeze dried and ground to a homogenous powder using mortar and pestle.</p>
<p>Sediment samples were taken with ROV-operated push cores and sliced into 1 cm sections. Frozen samples were freeze dried, acidified, and then used for isotopic analysis. Sediment traps were McLane Parflux traps using formalin fixed collection cups varying from 10 to 30 day sample integration. Swimmers were removed from the samples under a dissecting microscope prior to concentration, freeze drying, and acidification for isotopic analysis.</p>
<p>Compound specific isotopic analysis of amino acids followed the protocols outlined in Hannides et al 2013.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1829612 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1829612
completed
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
808-956-6567
Department of Oceanography, SOEST 1000 Pope Road
Honolulu
HI
96822
USA
jdrazen@hawaii.edu
pointOfContact
Brian N. Popp
University of Hawaiʻi at Mānoa
808-956-6206
Department of Earth Sciences 1680 East-West Road
Honolulu
HI
96822
USA
popp@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample
Cruise
Date_initial
Date_final
Site
Latitude
Longitude
Type
Species
Type_filter
Depth
d15N
d13C
ALA_N
GLY_N
THR_N
SER_N
VAL_N
LEU_N
ILE_N
PRO_N
ASX_N
GLX_N
PHE_N
TYR_N
LYS_N
ALA_N_SD
GLY_N_SD
THR_N_SD
SER_N_SD
VAL_N_SD
LEU_N_SD
ILE_N_SD
PRO_N_SD
ASX_N_SD
GLX_N_SD
PHE_N_SD
TYR_N_SD
LYS_N_SD
ALA_C
GLY_C
THR_C
SER_C
VAL_C
LEU_C
ILE_C
PRO_C
ASX_C
GLX_C
PHE_C
TYR_C
LYS_C
ALA_C_SD
GLY_C_SD
THR_C_SD
SER_C_SD
VAL_C_SD
LEU_C_SD
ILE_C_SD
PRO_C_SD
ASX_C_SD
GLX_C_SD
PHE_C_SD
TYR_C_SD
LYS_C_SD
Ekman cores
Brenke epibenthic sled
trace gas chromatograph (GC)
mortar and pestle
HOV Alvin
Thermo Scientific Delta V Plus IRMS
push cores
ROV Doc Ricketts
ROV Lu'ukai
scalpel
McLane Parflux traps
theme
None, User defined
sample identification
Cruise identfier
date
site
latitude
longitude
sample description
taxon
filter_size
depth
d15N measured in biota
d13C measured in biota
featureType
BCO-DMO Standard Parameters
Bottom Sediment Grab Samplers
Epibenthic Sled
Gas Chromatograph
Homogenizer
HOV Alvin
Isotope-ratio Mass Spectrometer
Push Corer
ROV Doc Ricketts
ROV Lu'ukai
scalpel
Sediment Trap
instrument
BCO-DMO Standard Instruments
AT42-10
Pulse 72
KM1914
KM2002
KM2008
service
Deployment Activity
Eastern North Pacific 34.50° N 123.06° W (Station M)
Station Aloha
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Assessing the relative importance of small vs large particles as sources of nutrition to abyssal communities
https://osprey.bco-dmo.org/project/775278
Collaborative Research: Assessing the relative importance of small vs large particles as sources of nutrition to abyssal communities
<p><em>NSF Award Abstract:</em><br />
The abyssal plains of the oceans cover roughly half of the earth's surface, host enormous reservoirs of biodiversity and mineral resources, and play important roles in nutrient recycling and carbon sequestration. The most important process controlling the structure and function of these ecosystems is the quantity and quality of food (mostly sinking organic particles) that reaches the deep-sea floor. However, we do not fully understand the processes provisioning this vast ecosystem. We propose to evaluate the relative importance of small and larger "marine snow" particles that sink to deep-sea benthic communities by using the stable isotope signature of amino acids within various food sources and trace their consumption by fauna on the seafloor. This project compares ecosystems from the productive waters off California with the nutrient poor central Pacific, north of Hawaii. This project provides novel insights into how surface ocean processes are coupled to food-webs at the deep ocean seafloor and how changes in food sources potentially impact deep-sea communities. This project also provides excellent training opportunities for graduate students, a postdoctoral researcher, and undergraduates at UH and USC, particularly underrepresented minorities who pursue majors in the geosciences. The project will sponsor an annual G6-12 teacher workshop to inform Hawaii educators about the deep sea and broadly disseminate knowledge to the community. All results are communicated broadly to inform the public as concerns regarding abyssal ecosystems are rising due to interests in deep-sea mining.</p>
<p>The most important process controlling the structure and function of abyssal ecosystems is the quantity and quality of organic material that ultimately reaches the deep-sea floor. Despite the strong relationship between euphotic zone export flux and benthic ecology, studies of abyssal ecosystems have observed a deficit between food supply and benthic community demand. Additional work is therefore needed, particularly with regards to understanding the sources of nutrition to the deep-sea benthos. Recent evidence suggests that small particles may be significant contributors to carbon export, increasing in relative importance with depth in the mesopelagic and reaching the abyssal seafloor. This project is to evaluate the relative importance of small and larger "marine snow" particles to deep-sea benthic communities using a combination of particle flux measurements and state of the art compound specific stable isotope analysis of amino acids (AA-CSIA) at two abyssal locations that contrast in overlying productivity, seasonality, and export magnitude. Time series measurements at these locations (Sta. M off California and Sta. Aloha off Hawaii) provide a rich context for the work. In the mesopelagic central North Pacific larger particles (>53 um) can be resolved from microbially reworked, smaller (0.7-53 um) particles using AA-CSIA. This project is characterizing the isotopic compositions of key individual compounds in a continuum of particle sizes (< 1.0 um suspended particles to large sinking particles >53 um) collected using in situ filtration near the seafloor and bottom-moored sediment traps, thereby defining source-specific isotopic signatures that can be traced into benthic fauna and sediments (that are collected by ROVs and epibenthic sleds). This research to understand pelagic-benthic coupling from particles to megafauna using isotopic measurements at the compound-level will yield novel insights into the importance of small microbially reworked particles to deep-sea benthic food webs. This will more precisely couple surface ocean processes to food-webs at the deep ocean seafloor with implications for understanding climate change effects and the efficiency of energy transfer to higher trophic levels. Furthermore, isotopic measurements can also be used to further parameterize ecosystem models by quantifying trophic position across size classes and thus estimate predator-prey mass ratios in relation to variation in body size spectra, functional type, and ultimately to carbon flux and remineralization. Finally, the results will help refine interpretations of deep-sea paleorecords of past nitrogen dynamics by calibrating potential changes in organic matter isotope values between the surface and seafloor archives.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Abyssal food web
largerWorkCitation
project
eng; USA
oceans
Eastern North Pacific 34.50° N 123.06° W (Station M); Station Aloha
-158
-123.06
22.6
34.5
2018-12-16
2020-08-03
California current, Station M (34º 50' N, 123º W) and North Pacific Subtropical Gyre, Station Aloha (22º 45' N, 158º W)
0
BCO-DMO catalogue of parameters from Compound specific isotope data of amino acids for abyssal macrofauna, megafauna, sediments, sediment traps, and in situ filtered particles at Station ALOHA off Hawaii and Station M off California from 2019 to 2020
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/922847.rdf
Name: Sample
Units: unitless
Description: <p>Sample identification code</p>
http://lod.bco-dmo.org/id/dataset-parameter/922848.rdf
Name: Cruise
Units: unitless
Description: <p>Cruise designation</p>
http://lod.bco-dmo.org/id/dataset-parameter/922849.rdf
Name: Date_initial
Units: unitless
Description: <p>Sampling initial date (UTC)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922850.rdf
Name: Date_final
Units: unitless
Description: <p>Sampling final date (UTC)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922851.rdf
Name: Site
Units: unitless
Description: <p>Site designation: StM = Station M; StALOHA = Station ALOHA</p>
http://lod.bco-dmo.org/id/dataset-parameter/922852.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude; positive values = North</p>
http://lod.bco-dmo.org/id/dataset-parameter/922853.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude; negative values = West</p>
http://lod.bco-dmo.org/id/dataset-parameter/922854.rdf
Name: Type
Units: unitless
Description: <p>Type of sample:<br />
Body = deposit feeder body tissue;<br />
Fgut = foregut, first 3rd of digestive tract;<br />
Filter = in situ filtered particles;<br />
Hgut = hindgut, last 3rd of digestive tract;<br />
Mgut = midgut, second 3rd of digestive tract;<br />
Sediment = surface sediment (0-5 mm)<br />
Sediment trap = particles from moored sediment trap array.</p>
http://lod.bco-dmo.org/id/dataset-parameter/922855.rdf
Name: Species
Units: unitless
Description: <p>Species of animal</p>
http://lod.bco-dmo.org/id/dataset-parameter/922857.rdf
Name: Type_filter
Units: unitless
Description: <p>Filter size</p>
http://lod.bco-dmo.org/id/dataset-parameter/922858.rdf
Name: Depth
Units: meters (m)
Description: <p>Depth of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/922859.rdf
Name: d15N
Units: ‰, vs AIR
Description: <p>Bulk nitrogen isotopic composition</p>
http://lod.bco-dmo.org/id/dataset-parameter/922860.rdf
Name: d13C
Units: ‰, vs VPDB
Description: <p>Bulk carbon isotopic composition</p>
http://lod.bco-dmo.org/id/dataset-parameter/922861.rdf
Name: ALA_N
Units: ‰, vs AIR
Description: <p>Mean d15N value of all injections for amino acid alanine (ALA)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922862.rdf
Name: GLY_N
Units: ‰, vs AIR
Description: <p>Mean d15N value of all injections for amino acid glycine (GLY)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922863.rdf
Name: THR_N
Units: ‰, vs AIR
Description: <p>Mean d15N value of all injections for amino acid threonine (THR)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922864.rdf
Name: SER_N
Units: ‰, vs AIR
Description: <p>Mean d15N value of all injections for amino acid serine (SER)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922865.rdf
Name: VAL_N
Units: ‰, vs AIR
Description: <p>Mean d15N value of all injections for amino acid valine (VAL)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922866.rdf
Name: LEU_N
Units: ‰, vs AIR
Description: <p>Mean d15N value of all injections for amino acid leucine (LEU)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922867.rdf
Name: ILE_N
Units: ‰, vs AIR
Description: <p>Mean d15N value of all injections for amino acid isoleucine (ILE)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922868.rdf
Name: PRO_N
Units: ‰, vs AIR
Description: <p>Mean d15N value of all injections for amino acid proline (PRO)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922869.rdf
Name: ASX_N
Units: ‰, vs AIR
Description: <p>Mean d15N value of all injections for amino acid aspartic Acid + asparagine</p>
http://lod.bco-dmo.org/id/dataset-parameter/922870.rdf
Name: GLX_N
Units: ‰, vs AIR
Description: <p>Mean d15N value of all injections for amino acid glutamic acid + glutamine</p>
http://lod.bco-dmo.org/id/dataset-parameter/922871.rdf
Name: PHE_N
Units: ‰, vs AIR
Description: <p>Mean d15N value of all injections for amino acid phenylalanine (PHE)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922872.rdf
Name: TYR_N
Units: ‰, vs AIR
Description: <p>Mean d15N value of all injections for amino acid tyrosine (TYR)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922873.rdf
Name: LYS_N
Units: ‰, vs AIR
Description: <p>Mean d15N value of all injections for amino acid lysine (LYS)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922874.rdf
Name: ALA_N_SD
Units: ‰, vs AIR
Description: <p>Standand deviation of d15N values from all injections for amino acid alanine (ALA)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922875.rdf
Name: GLY_N_SD
Units: ‰, vs AIR
Description: <p>Standand deviation of d15N values from all injections for amino acid glycine (GLY)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922876.rdf
Name: THR_N_SD
Units: ‰, vs AIR
Description: <p>Standand deviation of d15N values from all injections for amino acid threonine (THR)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922877.rdf
Name: SER_N_SD
Units: ‰, vs AIR
Description: <p>Standand deviation of d15N values from all injections for amino acid serine (SER)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922878.rdf
Name: VAL_N_SD
Units: ‰, vs AIR
Description: <p>Standand deviation of d15N values from all injections for amino acid valine (VAL)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922879.rdf
Name: LEU_N_SD
Units: ‰, vs AIR
Description: <p>Standand deviation of d15N values from all injections for amino acid leucine (LEU)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922880.rdf
Name: ILE_N_SD
Units: ‰, vs AIR
Description: <p>Standand deviation of d15N values from all injections for amino acid isoleucine (ILE)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922881.rdf
Name: PRO_N_SD
Units: ‰, vs AIR
Description: <p>Standand deviation of d15N values from all injections for amino acid proline (PRO)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922882.rdf
Name: ASX_N_SD
Units: ‰, vs AIR
Description: <p>Standand deviation of d15N values from all injections for amino acid aspartic acid + asparagine</p>
http://lod.bco-dmo.org/id/dataset-parameter/922883.rdf
Name: GLX_N_SD
Units: ‰, vs AIR
Description: <p>Standand deviation of d15N values from all injections for amino acid glutamic acid + glutamine</p>
http://lod.bco-dmo.org/id/dataset-parameter/922884.rdf
Name: PHE_N_SD
Units: ‰, vs AIR
Description: <p>Standand deviation of d15N values from all injections for amino acid phenylalanine (PHE)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922885.rdf
Name: TYR_N_SD
Units: ‰, vs AIR
Description: <p>Standand deviation of d15N values from all injections for amino acid tyrosine (TYR)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922886.rdf
Name: LYS_N_SD
Units: ‰, vs AIR
Description: <p>Standand deviation of d15N values from all injections for amino acid lysine (LYS)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922887.rdf
Name: ALA_C
Units: ‰, vs VPDB
Description: <p>Mean d13C value of all injections for amino acid alanine (ALA)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922888.rdf
Name: GLY_C
Units: ‰, vs VPDB
Description: <p>Mean d13C value of all injections for amino acid glycine (GLY)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922889.rdf
Name: THR_C
Units: ‰, vs VPDB
Description: <p>Mean d13C value of all injections for amino acid threonine (THR)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922890.rdf
Name: SER_C
Units: ‰, vs VPDB
Description: <p>Mean d13C value of all injections for amino acid serine (SER)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922891.rdf
Name: VAL_C
Units: ‰, vs VPDB
Description: <p>Mean d13C value of all injections for amino acid valine (VAL)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922892.rdf
Name: LEU_C
Units: ‰, vs VPDB
Description: <p>Mean d13C value of all injections for amino acid leucine (LEU)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922893.rdf
Name: ILE_C
Units: ‰, vs VPDB
Description: <p>Mean d13C value of all injections for amino acid isoleucine (ILE)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922894.rdf
Name: PRO_C
Units: ‰, vs VPDB
Description: <p>Mean d13C value of all injections for amino acid proline (PRO)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922895.rdf
Name: ASX_C
Units: ‰, vs VPDB
Description: <p>Mean d13C value of all injections for amino acid aspartic acid + asparagine</p>
http://lod.bco-dmo.org/id/dataset-parameter/922896.rdf
Name: GLX_C
Units: ‰, vs VPDB
Description: <p>Mean d13C value of all injections for amino acid glutamic acid + glutamine</p>
http://lod.bco-dmo.org/id/dataset-parameter/922897.rdf
Name: PHE_C
Units: ‰, vs VPDB
Description: <p>Mean d13C value of all injections for amino acid phenylalanine (PHE)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922898.rdf
Name: TYR_C
Units: ‰, vs VPDB
Description: <p>Mean d13C value of all injections for amino acid tyrosine (TYR)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922899.rdf
Name: LYS_C
Units: ‰, vs VPDB
Description: <p>Mean d13C value of all injections for amino acid lysine (LYS)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922900.rdf
Name: ALA_C_SD
Units: ‰, vs VPDB
Description: <p>Standand deviation of d13C values from all injections for amino acid alanine (ALA)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922901.rdf
Name: GLY_C_SD
Units: ‰, vs VPDB
Description: <p>Standand deviation of d13C values from all injections for amino acid glycine (GLY)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922902.rdf
Name: THR_C_SD
Units: ‰, vs VPDB
Description: <p>Standand deviation of d13C values from all injections for amino acid threonine (THR)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922903.rdf
Name: SER_C_SD
Units: ‰, vs VPDB
Description: <p>Standand deviation of d13C values from all injections for amino acid serine (SER)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922904.rdf
Name: VAL_C_SD
Units: ‰, vs VPDB
Description: <p>Standand deviation of d13C values from all injections for amino acid valine (VAL)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922905.rdf
Name: LEU_C_SD
Units: ‰, vs VPDB
Description: <p>Standand deviation of d13C values from all injections for amino acid leucine (LEU)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922906.rdf
Name: ILE_C_SD
Units: ‰, vs VPDB
Description: <p>Standand deviation of d13C values from all injections for amino acid isoleucine (ILE)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922907.rdf
Name: PRO_C_SD
Units: ‰, vs VPDB
Description: <p>Standand deviation of d13C values from all injections for amino acid proline (PRO)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922908.rdf
Name: ASX_C_SD
Units: ‰, vs VPDB
Description: <p>Standand deviation of d13C values from all injections for amino acid aspartic acid + asparagine</p>
http://lod.bco-dmo.org/id/dataset-parameter/922909.rdf
Name: GLX_C_SD
Units: ‰, vs VPDB
Description: <p>Standand deviation of d13C values from all injections for amino acid glutamic acid + glutamine</p>
http://lod.bco-dmo.org/id/dataset-parameter/922910.rdf
Name: PHE_C_SD
Units: ‰, vs VPDB
Description: <p>Standand deviation of d13C values from all injections for amino acid phenylalanine (PHE)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922911.rdf
Name: TYR_C_SD
Units: ‰, vs VPDB
Description: <p>Standand deviation of d13C values from all injections for amino acid tyrosine (TYR)</p>
http://lod.bco-dmo.org/id/dataset-parameter/922912.rdf
Name: LYS_C_SD
Units: ‰, vs VPDB
Description: <p>Standand deviation of d13C values from all injections for amino acid lysine (LYS)</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
42846
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/fd26e101-ffdc-45c1-8a82-d5f4d9f789be/content
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https://doi.org/10.26008/1912/bco-dmo.922789.1
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dataset
<p>Macrofauna and megafauna were collected in May and October 2019 using the HOV Alvin<em> </em>and the ROV Doc Ricketts, respectively, at Station M; and in July 2019, January 2020, and July 2020 using ROV Lu'ukai at Station Aloha. Macrofauna at Station M were collected using HOV-operated Ekman cores (20x20 centimeters (cm)). Macrofauna at Station Aloha were collected using a Brenke epibenthic sled towed across the seafloor due to the considerably lower macrofaunal densities at this oligotrophic site. Megafauna were collected using the submersible vehicle's manipulator arm and/or slurp gun. Upon retrieval to the surface, samples were placed in a cool room (5 degrees Celsius (ºC)) for further processing. Specimens of megafauna were weighed and measured, then they were dissected using a scalpel. All tissue samples were placed in cryovials and frozen in liquid nitrogen, and subsequently stored at -80ºC. Sieved (300 micrometers (um)) macrofauna samples were preserved in 10% buffered formalin. In the laboratory, macrofauna were further sorted by taxon. Samples of megafauna body tissues or macrofauna were freeze dried and ground to a homogenous powder using mortar and pestle.</p>
<p>Sediment samples were taken with ROV-operated push cores and sliced into 1 cm sections. Frozen samples were freeze dried, acidified, and then used for isotopic analysis. Sediment traps were McLane Parflux traps using formalin fixed collection cups varying from 10 to 30 day sample integration. Swimmers were removed from the samples under a dissecting microscope prior to concentration, freeze drying, and acidification for isotopic analysis.</p>
<p>Compound specific isotopic analysis of amino acids followed the protocols outlined in Hannides et al 2013.</p>
Specified by the Principal Investigator(s)
- Imported original file "Abyssal food web AACSIA.xlsx" into the BCO-DMO data system.
- Flagged "NA" as a missing data value. Missing data are empty/blank in the final CSV file.
- Converted date fields to YYYY-MM-DD format.
- Converted positive Longitude values to negative (to indicate the direction is west).
- Renamed fields to comply with BCO-DMO naming conventions.
- PI provided updated copy of file named as "922789_v1_compound_specific_isotope_dataV2.csv".
- Imported the new file, "922789_v1_compound_specific_isotope_dataV2.csv", into the BCO-DMO system; converted dates and renamed fields as described above.
- Saved the final file as "922789_v1_compound_specific_isotope_data.csv".
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
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Ekman cores
Ekman cores
PI Supplied Instrument Name: Ekman cores Instrument Name: Bottom Sediment Grab Samplers Instrument Short Name:BSGS Instrument Description: These samplers are designed to collect an accurate representative sample of the sediment bottom. The bite of the sampler should be deep enough so all depths are sampled equally. The closing mechanism is required to completely close and hold the sample as well as prevent wash-out during retrieval. Likewise, during descent the sampler should be designed to minimize disturbance of the topmost sediment by the pressure wave as it is lowered to the bottom. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/50/
Brenke epibenthic sled
Brenke epibenthic sled
PI Supplied Instrument Name: Brenke epibenthic sled Instrument Name: Epibenthic Sled Instrument Short Name: Instrument Description: An epibenthic sled is a semi-quantitative bottom-sampling device designed to trawl just above the bottom at the sediment water interface (the epibenthic zone). The sled consists of a rectangular steel frame with a mesh net (often more than one) attached to it. Towed along the ocean floor, its weight scrapes into the benthos, collecting any organisms on the surface or in the first few centimeters of sediment. It also collects the organisms in the water column just above the benthos.
Descriptions from WHOI and Census of Marine Life. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/24/
trace gas chromatograph (GC)
trace gas chromatograph (GC)
PI Supplied Instrument Name: trace gas chromatograph (GC) PI Supplied Instrument Description:TFA derivatives of amino acids were analyzed for stable N isotopic composition using a Thermo Scientific Delta V Plus IRMS interfaced to a trace gas chromatograph (GC) fitted with a 60 m BPx5 forte capillary column (0.32 mm internal diameter with 1.0 mm film thickness) through a GC–C III combustion furnace (980 ºC), reduction furnace (680 ºC), and liquid nitrogen cold trap. Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/
mortar and pestle
mortar and pestle
PI Supplied Instrument Name: mortar and pestle Instrument Name: Homogenizer Instrument Short Name:Homogenizer Instrument Description: A homogenizer is a piece of laboratory equipment used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others.
HOV Alvin
HOV Alvin
PI Supplied Instrument Name: HOV Alvin Instrument Name: HOV Alvin Instrument Short Name: Instrument Description: Human Occupied Vehicle (HOV) Alvin is part of the National Deep Submergence Facility (NDSF). Alvin enables in-situ data collection and observation by two scientists to depths reaching 6,500 meters, during dives lasting up to ten hours.
Commissioned in 1964 as one of the world’s first deep-ocean submersibles, Alvin has remained state-of-the-art as a result of numerous overhauls and upgrades made over its lifetime. The most recent upgrades, begun in 2011 and completed in 2021, saw the installation of a new, larger personnel sphere with a more ergonomic interior; improved visibility and overlapping fields of view; longer bottoms times; new lighting and high-definition imaging systems; improved sensors, data acquisition and download speed. It also doubled the science basket payload, and improved the command-and-control system allowing greater speed, range and maneuverability.
With seven reversible thrusters, it can hover in the water, maneuver over rugged topography, or rest on the sea floor. It can collect data throughout the water column, produce a variety of maps and perform photographic surveys. Alvin also has two robotic arms that can manipulate instruments, obtain samples, and its basket can be reconfigured daily based on the needs of the upcoming dive.
Alvin's depth rating of 6,500m gives researchers in-person access to 99% of the ocean floor. Alvin is a proven and reliable platform capable of diving for up to 30 days in a row before requiring a single scheduled maintenance day. Recent collaborations with autonomous vehicles such as Sentry have proven extremely beneficial, allowing PIs to visit promising sites to collect samples and data in person within hours of their being discovered, and UNOLs driven technological advances have improved the ability for scientific outreach and collaboration via telepresence
Alvin is named for Allyn Vine, a WHOI engineer and geophysicist who helped pioneer deep submergence research and technology.
(from https://www.whoi.edu/what-we-do/explore/underwater-vehicles/hov-alvin/, accessed 2022-09-09)
Thermo Scientific Delta V Plus IRMS
Thermo Scientific Delta V Plus IRMS
PI Supplied Instrument Name: Thermo Scientific Delta V Plus IRMS PI Supplied Instrument Description:TFA derivatives of amino acids were analyzed for stable N isotopic composition using a Thermo Scientific Delta V Plus IRMS interfaced to a trace gas chromatograph (GC) fitted with a 60 m BPx5 forte capillary column (0.32 mm internal diameter with 1.0 mm film thickness) through a GC–C III combustion furnace (980 ºC), reduction furnace (680 ºC), and liquid nitrogen cold trap. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
push cores
push cores
PI Supplied Instrument Name: push cores Instrument Name: Push Corer Instrument Short Name: Instrument Description: Capable of being performed in numerous environments, push coring is just as it sounds. Push coring is simply pushing the core barrel (often an aluminum or polycarbonate tube) into the sediment by hand. A push core is useful in that it causes very little disturbance to the more delicate upper layers of a sub-aqueous sediment.
Description obtained from: http://web.whoi.edu/coastal-group/about/how-we-work/field-methods/coring/
ROV Doc Ricketts
ROV Doc Ricketts
PI Supplied Instrument Name: ROV Doc Ricketts Instrument Name: ROV Doc Ricketts Instrument Short Name:Doc Ricketts Instrument Description: The remotely operated vehicle (ROV) Doc Ricketts is operated by the Monterey Bay Aquarium Research Institute (MBARI). ROV Doc Ricketts is capable of diving to 4000 meters (about 2.5 miles). The R/V Western Flyer is the support vessel for Doc Ricketts and was designed with a center well whose floor can be opened to allow Doc Ricketts to be launched from within the ship into the water below. For a complete description, see: https://www.mbari.org/at-sea/vehicles/remotely-operated-vehicles/rov-doc-ricketts/
ROV Lu'ukai
ROV Lu'ukai
PI Supplied Instrument Name: ROV Lu'ukai Instrument Name: ROV Lu'ukai Instrument Short Name: Instrument Description: The UH remotely operated vehicle (ROV) Lu'ukai is a small work-class ROV that can be operated from the R/V Kilo Moana to conduct investigations in waters up to 6,000 meters deep. After beginning operations in early 2018, the Lu'ukai has supported a study of deep-sea biodiversity and ecological processes in the western Clarion-Clipperton Zone, an area where numerous manganese nodule mining exploration claims are located; has maintained and upgraded sensors at the ALOHA Cabled Observatory, the world's deepest operating ocean observatory; and has recovered a failed telecommunications cable used to support acoustic research in local waters.
scalpel
scalpel
PI Supplied Instrument Name: scalpel Instrument Name: scalpel Instrument Short Name: Instrument Description: A scalpel, or lancet, or bistoury, is a small and extremely sharp bladed instrument used for dissection and surgery.
McLane Parflux traps
McLane Parflux traps
PI Supplied Instrument Name: McLane Parflux traps Instrument Name: Sediment Trap Instrument Short Name:Sediment Trap Instrument Description: Sediment traps are specially designed containers deployed in the water column for periods of time to collect particles from the water column falling toward the sea floor. In general a sediment trap has a jar at the bottom to collect the sample and a broad funnel-shaped opening at the top with baffles to keep out very large objects and help prevent the funnel from clogging. This designation is used when the specific type of sediment trap was not specified by the contributing investigator. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/33/
Cruise: AT42-10
AT42-10
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT42-10
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
Cruise: Pulse 72
Pulse 72
R/V Western Flyer
International Council for the Exploration of the Sea
R/V Western Flyer
vessel
Pulse 72
Kenneth Smith
Monterey Bay Aquarium Research Institute
Cruise: KM1914
KM1914
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM1914
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
Cruise: KM2002
KM2002
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM2002
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
Cruise: KM2008
KM2008
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM2008
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
R/V Western Flyer
International Council for the Exploration of the Sea
R/V Western Flyer
vessel
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel