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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/922818.rdf" xlink:actuate="onRequest">Macrophytes' amelioration of seawater acidity: Comparison among four species of marine macrophytes (Ulva lactuca, Zostera marina, Fucus vesiculosus and Saccharina latissima) from the Gulf of Maine from June to July 2015</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Ricart, A. M., Price, N. N., Honisch, B. (2024) Macrophytes' amelioration of seawater acidity: Comparison among four species of marine macrophytes (Ulva lactuca, Zostera marina, Fucus vesiculosus and Saccharina latissima) from the Gulf of Maine from June to July 2015. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-08-22 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.922818.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Dataset Description: &amp;lt;p&amp;gt;Data from a related experiment (experiment 2, residence time and irradiance)&amp;amp;nbsp;on Saccharina latissima is available through BCO-DMO at https://www.bco-dmo.org/dataset/922819.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;&amp;lt;span style=&amp;quot;font-size:14px&amp;quot;&amp;gt;&amp;lt;span style=&amp;quot;font-family:arial,helvetica,sans-serif&amp;quot;&amp;gt;Methodology summary: The experiment in aquaria measured changes in dissolved oxygen, pH, and total alkalinity, and derived resultant changes to dissolved inorganic carbon (DIC) and calcium carbonate saturation state (Ω).&amp;lt;/span&amp;gt;&amp;lt;/span&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;span style=&amp;quot;font-size:14px&amp;quot;&amp;gt;&amp;lt;span style=&amp;quot;font-family:arial,helvetica,sans-serif&amp;quot;&amp;gt;Values of temperature, salinity, oxygen and pH were recorded in all the chambers using a multiparameter meter (HQ40D, Hach Lange) calibrated before measurements with TRIS buffer, from A. Dickson laboratory, with an accuracy of 0.01 pH units. Discrete water samples were acquired from three representative chambers from each CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; level per species for analysis of total alkalinity (AT). Seawater AT was measured using open-cell titration with triplicate samples (Metrohm 855 Robotic Titrosampler, Metrohm, USA) using 0.1 N HCl (Fisher Chemical) diluted to a nominal concentration of 0.0125 M. Acid was calibrated by analyzing Certified Reference Material (CRM Batch 138) from A. Dickson’s laboratory. For each set of triplicate analyses, the median value was considered. Instrumental precision was within 5 µmol kg -1.&amp;lt;/span&amp;gt;&amp;lt;/span&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;span style=&amp;quot;background-color:transparent; font-family:arial,sans-serif; font-size:10.5pt&amp;quot;&amp;gt;See detailed methodology described in the manuscript: Optimizing marine macrophyte capacity to locally ameliorate ocean acidification under variable light and flow regimes: Insights from an experimental approach (Ricart, A. M. et al. 2023).&amp;lt;/span&amp;gt;&amp;lt;span style=&amp;quot;font-size:14px&amp;quot;&amp;gt;&amp;lt;span style=&amp;quot;font-family:arial,helvetica,sans-serif&amp;quot;&amp;gt; &amp;lt;/span&amp;gt;&amp;lt;/span&amp;gt;&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/923077.rdf" xlink:title="EAR-1460861" xlink:actuate="onRequest">Funding provided by NSF Division of Earth Sciences (NSF EAR) Award Number: EAR-1460861 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1460861</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/923082.rdf" xlink:title="OCE-1156740" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1156740 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1156740</gmx:Anchor>
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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/922978.rdf" xlink:title="ORCID" xlink:actuate="onRequest">Brittney Honisch</gmx:Anchor>
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	Name: Sample
	Units: unitless
	Description: &lt;p&gt;Chamber ID&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934058.rdf
	Name: Species
	Units: unitless
	Description: &lt;p&gt;The scientific name of the marine macrophyte species used and Control = no kelp present&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934059.rdf
	Name: lat
	Units: decimal degrees
	Description: &lt;p&gt;Sampling location latitude, south is negative&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934060.rdf
	Name: lon
	Units: decimal degrees
	Description: &lt;p&gt;Sampling location longitude, west is negative&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934061.rdf
	Name: date_start
	Units: unitless
	Description: &lt;p&gt;Collection start date&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934062.rdf
	Name: date_end
	Units: unitless
	Description: &lt;p&gt;Collection end date&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934063.rdf
	Name: Treatment
	Units: microatmospheres
	Description: &lt;p&gt;pCO2 level during incubation&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934064.rdf
	Name: Liters
	Units: liters (L)
	Description: &lt;p&gt;volume of incubation chamber&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934065.rdf
	Name: Hours
	Units: unitless
	Description: &lt;p&gt;total duration of incubation&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934066.rdf
	Name: Dry_Weight
	Units: grams (g)
	Description: &lt;p&gt;dry biomass of macrophyte inside the chamber. Empty values = not available&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934067.rdf
	Name: Salinity
	Units: unitless
	Description: &lt;p&gt;salinity of seawater&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934068.rdf
	Name: Temperature
	Units: degrees Celcius (°C)
	Description: &lt;p&gt;temperature of seawater&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934069.rdf
	Name: Initial_Dissolved_Oxygen
	Units: micromolar per liter (uM/L)
	Description: &lt;p&gt;Initial dissolved oxygen&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934070.rdf
	Name: Initial_pH
	Units: total scale
	Description: &lt;p&gt;initial pH&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934071.rdf
	Name: Initial_ALK
	Units: micromolar per kilogram (uM/kg)
	Description: &lt;p&gt;initial total alkalinity&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934072.rdf
	Name: Initial_pCO2
	Units: microatmospheres
	Description: &lt;p&gt;initial pCO2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934073.rdf
	Name: delta_DO_norm
	Units: unitless
	Description: &lt;p&gt;The difference in dissolved oxygen before and after the incubations normalized&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934074.rdf
	Name: delta_DIC_norm
	Units: unitless
	Description: &lt;p&gt;The difference in dissolved inorganic carbon before and after the incubations normalized&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934075.rdf
	Name: delta_pH_norm
	Units: unitless
	Description: &lt;p&gt;The difference in pH before and after the incubations normalized&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/934076.rdf
	Name: delta_omega_norm
	Units: unitless
	Description: &lt;p&gt;The difference in omega before and after the incubations normalized. Omega is the saturation state of calcium carbonate (CaCO3), the degree to which seawater is saturated with CaCO3. This essentially is a measure of carbonate ion concentration.&lt;/p&gt; 
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&amp;lt;p&amp;gt;&amp;lt;span style=&amp;quot;font-size:14px&amp;quot;&amp;gt;&amp;lt;span style=&amp;quot;font-family:arial,helvetica,sans-serif&amp;quot;&amp;gt;Values of temperature, salinity, oxygen and pH were recorded in all the chambers using a multiparameter meter (HQ40D, Hach Lange) calibrated before measurements with TRIS buffer, from A. Dickson laboratory, with an accuracy of 0.01 pH units. Discrete water samples were acquired from three representative chambers from each CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; level per species for analysis of total alkalinity (AT). Seawater AT was measured using open-cell titration with triplicate samples (Metrohm 855 Robotic Titrosampler, Metrohm, USA) using 0.1 N HCl (Fisher Chemical) diluted to a nominal concentration of 0.0125 M. Acid was calibrated by analyzing Certified Reference Material (CRM Batch 138) from A. Dickson’s laboratory. For each set of triplicate analyses, the median value was considered. Instrumental precision was within 5 µmol kg -1.&amp;lt;/span&amp;gt;&amp;lt;/span&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;span style=&amp;quot;background-color:transparent; font-family:arial,sans-serif; font-size:10.5pt&amp;quot;&amp;gt;See detailed methodology described in the manuscript: Optimizing marine macrophyte capacity to locally ameliorate ocean acidification under variable light and flow regimes: Insights from an experimental approach (Ricart, A. M. et al. 2023).&amp;lt;/span&amp;gt;&amp;lt;span style=&amp;quot;font-size:14px&amp;quot;&amp;gt;&amp;lt;span style=&amp;quot;font-family:arial,helvetica,sans-serif&amp;quot;&amp;gt; &amp;lt;/span&amp;gt;&amp;lt;/span&amp;gt;&amp;lt;/p&amp;gt;</gco:CharacterString>
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&amp;lt;p&amp;gt;Carbonate system calculations were performed using the seacarb R package&amp;amp;nbsp;and assuming published values for constants K1 and K2, KF, and KS.&amp;amp;nbsp;Uncertainties of the derived parameters (DIC, pCO2, and Ω) were quantified using a Monte Carlo analysis (100 simulations). For each simulation, normally distributed errors were introduced into pH (± 0.01) and AT (± 5 μmol kg−1). The overall uncertainties of the derived parameters were calculated as one standard deviation of the simulations. On average, uncertainties for DIC were 0.28 %, for pCO2 were 2.5 %&amp;amp;nbsp;and for Ω 0.01 %.&amp;amp;nbsp;For pCO2, uncertainty is higher at lower pCO2, whereas uncertainty is lower for Ω at lower Ω. The uncertainty for DIC was similar to the uncertainty in AT, that is ± 5 μmol kg−1.&amp;lt;/p&amp;gt;</gco:CharacterString>
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The first column of the submitted dataset file, data_to_publish_exp1.csv, did not have a parameter name, and it stood for the row number of each line of the file. Removed this parameter column from the final dataset since a spreadsheet viewer will show the row numbers.

Removed units from the parameter names since these units will be noted in the parameters section of the dataset page.

Replaced NA values with blanks in the dry_weight column.

Renamed Ulva to Ulva lactuca to match the pattern of the other species entries in the dataset.

Renamed Z.marina to Zostera marina to match the format of the other species entries in the dataset.

Joined a metadata table created by the data manager to the submitted dataset on the parameter name Species to include metadata in the primary dataset file. The metadata table has columns of Species, lat, lon, date_start, date_end.

Reordered the parameters so that the metadata is at the front of the dataset. 

------------------------------

Checked taxonomy of dataset.

Checked that all the species names in the dataset file matched those found in WoRMS.

Created a Species WoRMS taxonomy file species_list_experiment_1.csv by using the WoRMS website to create a table of the species name with the following columns: ScientificName, AphiaID, LSID, Authority, Kingdom, Phylum, Class, Order, Family, Genus, Species.</gco:CharacterString>
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