http://lod.bco-dmo.org/id/dataset/922819 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2024-03-19 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Macrophytes' amelioration of seawater acidity: Water residence time and irradiance of Saccharina latissima from the Gulf of Maine in November 2018 2024-08-22 publication 2024-08-22 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2024-08-23 publication https://doi.org/10.26008/1912/bco-dmo.922819.1 Nichole N. Price Bigelow Laboratory for Ocean Sciences principalInvestigator Brittney Honisch Bigelow Laboratory for Ocean Sciences principalInvestigator Aurora M Ricart Bigelow Laboratory for Ocean Sciences principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Ricart, A. M., Price, N. N., Honisch, B. (2024) Macrophytes' amelioration of seawater acidity: Water residence time and irradiance of Saccharina latissima from the Gulf of Maine in November 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-08-21 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.922819.1 [access date] Dataset Description: &lt;p&gt;Data from a related experiment (experiment 1, comparison among species)&amp;nbsp;is available through BCO-DMO at https://www.bco-dmo.org/dataset/922818.&lt;/p&gt; Methods and Sampling: &lt;p&gt;&lt;span style=&quot;font-family:arial,helvetica,sans-serif&quot;&gt;Methodology summary: The experiment in aquaria measured changes in dissolved oxygen, pH, and total alkalinity, and derived resultant changes to dissolved inorganic carbon (DIC) and calcium carbonate saturation state (Ω).&lt;/span&gt;&lt;/p&gt; &lt;p&gt;&lt;span style=&quot;font-family:arial,helvetica,sans-serif&quot;&gt;Values of temperature, salinity and oxygen were measured using a multiparameter meter (HQ40D, Hach Lange). Discrete water samples were acquired from each tank for analysis of pH and total alkalinity (AT) that were run the same day of the experiment. Seawater pH of the discrete water samples was determined on the total scale as per Dickson et al. 2007 with a high-precision spectrophotometer (Agilent Cary 8454 with PCB 1500 Water Peliter System) using unpurified m-cresol purple dye (Alfa Aesar A180025-06). Water samples were kept in a temperature-controlled water bath at 20°C before analysis, and during analysis using a thermostatic cell holder, to minimize temperature-induced errors in absorbance measurements. The spectrophotometer was validated by analyzing TRIS buffer revealing that the system was accurate to within 0.005 pH units. Seawater AT was measured using a CONTROS HydroFIA TA® instrument (4H-Jena GmbH, Jena Germany), which performed a single-point titration of seawater with 0.1N HCl, using bromocresol green as the indicator for spectrophotometric pH detection. HCl titrant was calibrated with Certified Reference Material (Batch 172) from A. Dickson’s laboratory. Instrumental precision was within 5 µmol kg -1.&lt;/span&gt;&lt;/p&gt; &lt;p&gt;&lt;span style=&quot;font-family:arial,helvetica,sans-serif&quot;&gt;See detailed methodology described in the manuscript: Optimizing marine macrophyte capacity to locally ameliorate ocean acidification under variable light and flow regimes: Insights from an experimental approach (Ricart, A. M. et al. 2023).&lt;/span&gt;&lt;/p&gt; Funding provided by NSF Division of Earth Sciences (NSF EAR) Award Number: EAR-1460861 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1460861 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1156740 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1156740 completed Nichole N. Price Bigelow Laboratory for Ocean Sciences 60 Bigelow Dr, East Boothbay ME 04544 USA nprice@bigelow.org pointOfContact Brittney Honisch Bigelow Laboratory for Ocean Sciences 60 Bigelow Dr. East Boothbay ME 04544 USA bhonisch@bigelow.org pointOfContact Aurora M Ricart Bigelow Laboratory for Ocean Sciences 60 Bigelow Dr. East Boothbay ME 04544 USA aricart@bigelow.org pointOfContact asNeeded Dataset Version: 1 Unknown Species lat lon Date Aquaria Future_Ambient Kelp_Control Kelp_biomass_wet Kelp_biomass_dry PAR Flow_level Salinity Temperature Inflow_Dissolved_oxygen Inflow_pH Inflow_ALK delta_DO delta_DIC delta_pH delta_omega_ar PCB 1500 Water Peliter System multiparameter meter (HQ40D, Hach Lange) Agilent Cary 8454 CONTROS HydroFIA® TA theme None, User defined species scientific name (binomial) latitude longitude date replicate treatment wet_weight_biomass dry_weight_biomass PAR salinity water temperature at measurement depth dissolved Oxygen pH total alkalinity (TA) No BCO-DMO term featureType BCO-DMO Standard Parameters circulating water bath Multi Parameter Portable Meter Spectrophotometer Total alkalinity flow through analyzer instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. REU Site: Bigelow Laboratory for Ocean Sciences - Undergraduate Research Experience in the Gulf of Maine and the World Ocean https://osprey.bco-dmo.org/project/923078 REU Site: Bigelow Laboratory for Ocean Sciences - Undergraduate Research Experience in the Gulf of Maine and the World Ocean <p><strong>NSF Award Abstract:</strong></p> <p>Bigelow Laboratory for Ocean Sciences, in West Boothbay Harbor - Maine, plans to implement an REU site that will support 10 students each year, for the next 5 years. This REU program will combine group learning and guided individual research to help students address societal issues concerning the oceans. The goals of this REU Program are i) to provide undergraduate students from around the United States mentoring and access to high quality, hands on research opportunities in Oceanography and the opportunity to understand its interdisciplinary nature, ii) improve the capability and confidence of students to learn independently, iii) help to prepare undergraduates for their professional careers, and iv) increase the participation of underrepresented minorities in marine science careers. REU students will be incorporated into research groups consisting of technicians, post-docs, and junior and senior scientists, gaining exposure to the suite of activities and personalities involved in a science career. The recruitment goal is to engage students from all groups underrepresented in ocean sciences, such that cohorts are ~ 40% underrepresented students (including 1st generation college students). To achieve the recruitment goals, the principal investigator will work with partner organizations, like the Institute for Broadening Participation and Maine Community College System. Program content and delivery will continually be refined based on feedback from students and mentors in the form of pre-program, midpoint and final evaluations.</p> <p>As a group, students will participate in a sampling cruise on the local estuary, and attend weekly meetings to learn the fundamentals of the scientific process, such as how to form testable hypotheses, critically evaluate data, and present information in a scientific manner to their peers and to the public. Students will attend an ethics in science discussion, a laboratory safety course with risk assessment training and a concept mapping workshop during the early stages of their project. Students participate in and become familiar with public outreach through attendance at Bigelow's weekly Café Scientifique talks (for the general public), participation in the Lab's Open House and frequent public tours.</p> <p><strong>Continuing Award</strong></p> Bigelow REU GOM largerWorkCitation project eng; USA oceans -69.57825 -69.57825 43.860556 43.860556 2018-11-01 2018-11-01 Gulf of Maine, US 0 BCO-DMO catalogue of parameters from Macrophytes' amelioration of seawater acidity: Water residence time and irradiance of Saccharina latissima from the Gulf of Maine in November 2018 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/934079.rdf Name: Species Units: unitless Description: <p>The scientific name of the marine macrophyte species used and Control = no kelp present</p> http://lod.bco-dmo.org/id/dataset-parameter/934080.rdf Name: lat Units: decimal degrees Description: <p>Sampling location latitude, south is negative</p> http://lod.bco-dmo.org/id/dataset-parameter/934081.rdf Name: lon Units: decimal degrees Description: <p>Sampling location longitude, west is negative</p> http://lod.bco-dmo.org/id/dataset-parameter/934082.rdf Name: Date Units: unitless Description: <p>date of experiment</p> http://lod.bco-dmo.org/id/dataset-parameter/934083.rdf Name: Aquaria Units: unitless Description: <p>replicate aquaria</p> http://lod.bco-dmo.org/id/dataset-parameter/934084.rdf Name: Future_Ambient Units: unitless Description: <p>environmental conditions treatments (ambient conditions vs future conditions of temperature and pCO2 in seawater). Values of Ambient or Future.</p> http://lod.bco-dmo.org/id/dataset-parameter/934085.rdf Name: Kelp_Control Units: unitless Description: <p>presence of kelp within the aquaria. Kelp = kelp present. Control = no kelp present</p> http://lod.bco-dmo.org/id/dataset-parameter/934086.rdf Name: Kelp_biomass_wet Units: grams (g) Description: <p>wet biomass of kelp</p> http://lod.bco-dmo.org/id/dataset-parameter/934087.rdf Name: Kelp_biomass_dry Units: grams (g) Description: <p>dry biomass of kelp</p> http://lod.bco-dmo.org/id/dataset-parameter/934088.rdf Name: PAR Units: micromols m-2 s-1 (uM m-2 s-1) Description: <p>photosynthetic active radiation</p> http://lod.bco-dmo.org/id/dataset-parameter/934089.rdf Name: Flow_level Units: unitless Description: <p>residence time treatments (High or Low)</p> http://lod.bco-dmo.org/id/dataset-parameter/934090.rdf Name: Salinity Units: unitless Description: <p>salinity of seawater</p> http://lod.bco-dmo.org/id/dataset-parameter/934091.rdf Name: Temperature Units: degrees Celsius (°C) Description: <p>temperature of seawater</p> http://lod.bco-dmo.org/id/dataset-parameter/934092.rdf Name: Inflow_Dissolved_oxygen Units: micromolar per liter (uM/L) Description: <p>Initial dissolved oxygen</p> http://lod.bco-dmo.org/id/dataset-parameter/934093.rdf Name: Inflow_pH Units: unitless Description: <p>initial pH. Empty values = not available</p> http://lod.bco-dmo.org/id/dataset-parameter/934094.rdf Name: Inflow_ALK Units: micromolar per kilogram (uM/kg) Description: <p>initial total alkalinity</p> http://lod.bco-dmo.org/id/dataset-parameter/934095.rdf Name: delta_DO Units: unitless Description: <p>difference in dissolved oxygen between aquaria inflow and outflow</p> http://lod.bco-dmo.org/id/dataset-parameter/934096.rdf Name: delta_DIC Units: unitless Description: <p>difference in dissolved inorganic carbon between aquaria inflow and outflow. Empty values = not available</p> http://lod.bco-dmo.org/id/dataset-parameter/934097.rdf Name: delta_pH Units: unitless Description: <p>The difference in pH between aquaria inflow and outflow. Empty values = not available</p> http://lod.bco-dmo.org/id/dataset-parameter/934098.rdf Name: delta_omega_ar Units: unitless Description: <p>The difference in omega between aquaria inflow and outflow. Omega is the saturation state of calcium carbonate (CaCO3), the degree to which seawater is saturated with CaCO3. This essentially is a measure of carbonate ion concentration. Empty values = not available</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 16933 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/cbeab205-c82e-4ff2-9672-a3623be27bc6/content download https://doi.org/10.26008/1912/bco-dmo.922819.1 download onLine dataset &lt;p&gt;&lt;span style=&quot;font-family:arial,helvetica,sans-serif&quot;&gt;Methodology summary: The experiment in aquaria measured changes in dissolved oxygen, pH, and total alkalinity, and derived resultant changes to dissolved inorganic carbon (DIC) and calcium carbonate saturation state (Ω).&lt;/span&gt;&lt;/p&gt; &lt;p&gt;&lt;span style=&quot;font-family:arial,helvetica,sans-serif&quot;&gt;Values of temperature, salinity and oxygen were measured using a multiparameter meter (HQ40D, Hach Lange). Discrete water samples were acquired from each tank for analysis of pH and total alkalinity (AT) that were run the same day of the experiment. Seawater pH of the discrete water samples was determined on the total scale as per Dickson et al. 2007 with a high-precision spectrophotometer (Agilent Cary 8454 with PCB 1500 Water Peliter System) using unpurified m-cresol purple dye (Alfa Aesar A180025-06). Water samples were kept in a temperature-controlled water bath at 20°C before analysis, and during analysis using a thermostatic cell holder, to minimize temperature-induced errors in absorbance measurements. The spectrophotometer was validated by analyzing TRIS buffer revealing that the system was accurate to within 0.005 pH units. Seawater AT was measured using a CONTROS HydroFIA TA® instrument (4H-Jena GmbH, Jena Germany), which performed a single-point titration of seawater with 0.1N HCl, using bromocresol green as the indicator for spectrophotometric pH detection. HCl titrant was calibrated with Certified Reference Material (Batch 172) from A. Dickson’s laboratory. Instrumental precision was within 5 µmol kg -1.&lt;/span&gt;&lt;/p&gt; &lt;p&gt;&lt;span style=&quot;font-family:arial,helvetica,sans-serif&quot;&gt;See detailed methodology described in the manuscript: Optimizing marine macrophyte capacity to locally ameliorate ocean acidification under variable light and flow regimes: Insights from an experimental approach (Ricart, A. M. et al. 2023).&lt;/span&gt;&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;DIC and Ω, as aragonite saturation state, were calculated from pH and AT.&lt;/p&gt; &lt;p&gt;Carbonate system calculations were performed using the seacarb R package&amp;nbsp;and assuming published values for constants K1 and K2, KF, and KS.&amp;nbsp;Uncertainties of the derived parameters (DIC, pCO2, and Ω) were quantified using a Monte Carlo analysis (100 simulations). For each simulation, normally distributed errors were introduced into pH (± 0.01) and AT (± 5 μmol kg−1). The overall uncertainties of the derived parameters were calculated as one standard deviation of the simulations. On average, uncertainties for DIC were 0.28 %, for pCO2 were 2.5 %&amp;nbsp;and for Ω 0.01 %.&amp;nbsp;For pCO2, uncertainty is higher at lower pCO2, whereas uncertainty is lower for Ω at lower Ω. The uncertainty for DIC was similar to the uncertainty in AT, that is ± 5 μmol kg−1.&lt;/p&gt; Specified by the Principal Investigator(s) Loaded the submitted file data_to_publish_exp2.csv into Laminar (BCO-DMO data file processor) The first column of the submitted dataset file, data_to_publish_exp2.csv, did not have a parameter name, and it stood for the row number of each line of the file. Removed this parameter column from the final dataset since a spreadsheet viewer will show the row numbers. Replaced periods with underscores in the parameter names to follow BCO-DMO parameter naming guidelines. Removed units from the parameter names because they will be noted in the parameters section of the dataset page. Replaced NA values with blanks in the Inflow_pH, delta_DIC, delta_pH, and delta_omega_ar columns. Added a Species parameter value with ‘Saccharina latissima’ and ‘Control’ whenever the row value of Kelp_Control is ‘Control’. This is to join on the parameter Species in a data manager created metadata table with the columns of Species, and lat, lon. Joined a metadata table created by the data manager to the submitted dataset on the parameter Species to include metadata in the primary dataset file. Converted the date format to the ISO 8601 format %Y-%m-%d Reordered the parameters so the metadata is at the front of the dataset. ------------------------------ Checked taxonomy of dataset. Checked that all the species names in the dataset file matched those found in WoRMS. Created a Species WoRMS taxonomy file species_list_experiment_2.csv by using the WoRMS website to create a table of the species name with the following columns: ScientificName, AphiaID, LSID, Authority, Kingdom, Phylum, Class, Order, Family, Genus, Species. Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact PCB 1500 Water Peliter System PCB 1500 Water Peliter System PI Supplied Instrument Name: PCB 1500 Water Peliter System Instrument Name: circulating water bath Instrument Short Name: Instrument Description: A device designed to regulate the temperature of a vessel by bathing it in water held at the desired temperature. [Definition Source: NCI]  multiparameter meter (HQ40D, Hach Lange) multiparameter meter (HQ40D, Hach Lange) PI Supplied Instrument Name: multiparameter meter (HQ40D, Hach Lange) PI Supplied Instrument Description:Designed for water quality applications measuring pH, Conductivity, TDS, Salinity, Dissolved Oxygen (DO), ORP and ISE, the Hach HQ40D portable multi meter is a two channels advanced handheld digital meter.  Instrument Name: Multi Parameter Portable Meter Instrument Short Name: Instrument Description: An analytical instrument that can measure multiple parameters, such as pH, EC, TDS, DO and temperature with one device and is portable or hand-held. Agilent Cary 8454 Agilent Cary 8454 PI Supplied Instrument Name: Agilent Cary 8454 PI Supplied Instrument Description:The Agilent Cary Diode Array Spectrophotometer 8454 measures a full spectrum, in as little as 0.1 seconds. Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/ CONTROS HydroFIA® TA CONTROS HydroFIA® TA PI Supplied Instrument Name: CONTROS HydroFIA® TA PI Supplied Instrument Description:A defined amount of seawater is acidified by injection of a fixed amount of hydrochloric acid (HCl). After acidification the generated CO₂ in the sample is removed by means of a membrane based degassing unit resulting in a so-called open-cell titration. The subsequent pH determination is carried out by means of an indicator dye (Bromocresol green) and VIS absorption spectrometry. Together with salinity and temperature, the resulting pH is directly used for the calculation of total alkalinity. Instrument Name: Total alkalinity flow through analyzer Instrument Short Name:TA - flow analyzer Instrument Description: A flow through system for the determination of the total alkalinity in seawater. Its general principle is based on open-cell single-point titration with spectrophotometric pH determination. It can be used for continuous monitoring during surface water applications as well as for discrete sample measurements.