http://lod.bco-dmo.org/id/dataset/923000
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-03-20
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Copepod Pressure Vessel Experiments from samples collected from the East Pacific Rise during ROV SuBastian deployments and R/V Falkor (too) cruise FKt230627 in June-July 2023
2024-03-20
publication
2024-03-20
revision
BCO-DMO Linked Data URI
2024-03-20
creation
http://lod.bco-dmo.org/id/dataset/923000
Sabine Gollner
Royal Netherlands Institute for Sea Research
principalInvestigator
Monika Bright
University of Vienna
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Bright, M., Gollner, S., Messora, A. (2024) Copepod Pressure Vessel Experiments from samples collected from the East Pacific Rise during ROV SuBastian deployments and R/V Falkor (too) cruise FKt230627 in June-July 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-03-20 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/923000 [access date]
EPR Vent Copepods Pressure Experiment Dataset Description: <p>&nbsp;</p>
<p>&nbsp;</p> Methods and Sampling: <p>Specimens were collected with ROV SuBastian at 9°N 50’N and 104°W along the&nbsp;East Pacific Rise in the Tica vent area and sites Bio9 and Biovent (~2500 m depth) during expedition FKt230627 in July 2023 aboard R/V Falkor(too).&nbsp;</p>
<p>Deep-sea hydrothermal vent copepods were sampled from two different habitats: diffuse flow habitats dominated by&nbsp;<em>Riftia pachyptila</em>&nbsp;tubeworms and&nbsp;<em>Bathymodiolus thermophilus</em>&nbsp;mussels, and focused flow habitats dominated by&nbsp;<em>Alvinella pompejana</em>&nbsp;pompeii worms. A suction sampler and/or ROV manipulator grabs were used to sample detritus and megafauna respectively. These samples were then sorted for copepods and subsampled to be used for a total of 118 LT<sub>50</sub>/LD<sub>50</sub>&nbsp;exposure incubations performed on board the research vessel. Each incubation contained 3 replicate vials with 10 copepods each, for a total of 3540 copepods.</p>
<p>Diverse exposure times, temperatures, pressures, and oxic/anoxic conditions were selected to explore the tolerance of deep-sea hydrothermal vent copepods in relation to habitat, temperature, oxygen concentration and pressure.&nbsp;Exposure times were the following: 2h, 4h, 8h, 10h. Temperature ranges for each incubation were established based on the ROV’s temperature probe readings. A control temperature of 4°C was always included to represent similar thermal conditions to the ambient bottom water around hydrothermal vents. The other temperatures (12, 22, 25, 28, 31, 34, 37, 40, 43°C) were selected to obtain a complete survival curve that ranged from ~100% survival at the lowest temperature to ~0% at the highest temperature for each habitat/time/oxygen/pressure treatment combination. Two oxygen concentration conditions were tested: oxic (163.88 ± 5.82 µM before and 95.63 ± 9.14 µM after incubations) and anoxic (0.24 ± 0.11 µM before and 1.76 ± 0.38 µM after incubations). Concentrations were monitored before and after incubations using self-adhesive Oxygen Sensor Spots mounted on the inside of the incubation vials (SP-PSt3-SA) and a fiber-optic oxygen meter (Fibox 4).</p>
<p>Ten live copepods were transferred using a pipette to each seawater-filled 5.9 ml glass vial&nbsp;(Exetainer 719W) equipped with an airtight pressure relief cap. The glass vials were placed into pressure vessels and pressurized up to 200 bar (3000 psi) using a Waters 515 HPLC Pump, and then placed in a water bath (ARGOLAB WB 12 Lt) to keep a constant temperature. At the end of each incubation the vessel was depressurized, and the content of each vial was examined under a Leica EZ4 W stereomicroscope to record the number of living/dead copepods. After the experiment, dead copepod specimens were not disposed, but instead were fixed in 95% Ethanol for preservation.</p>
<p>The data was used to estimate the median lethal temperature (LD<sub>50</sub>) corresponding to each exposure time (LT<sub>50</sub>) by fitting a survival curve to the temperature and respective copepod survival data using Nonlinear Least Squares regression and isolating the theoretical 50% survival temperature.</p>
Funding provided by Schmidt Ocean Institute (SOI) Award Number: FKt230627
Funding provided by Netherlands Organization of Scientific Research (NWO, The Hague, The Netherlands) Award Number: OCENW.M.22.080 (Subsurface life at deep-sea hydrothermal vents) Award URL: https://www.nwo.nl/en/projects/ocenwm22080
onGoing
Sabine Gollner
Royal Netherlands Institute for Sea Research
Landsdiep 4 1797 SZ 't Horntje
Netherlands
Sabine.Gollner@nioz.nl
pointOfContact
Monika Bright
University of Vienna
+43 1 4277 76430
Department of Functional and Evolutionary Ecology Djerassiplatz 1
Vienna
Austria
monika.bright@univie.ac.at
pointOfContact
asNeeded
Dataset Version: 1
Unknown
ARGOLAB WB 12 Lt
Leica EZ4 W Compound Microscope
ROV SuBastian Multi-Chamber Suction Sampler
Fibox 4 stand-alone fiber optic oxygen meter
Self-adhesive Oxygen Sensor Spot SP-PSt3-SA
customized pressure vessel
Waters 515 HPLC Pump
ROV SuBastian
theme
None, User defined
ARGOLAB Water Bath 12 Liters
Microscope - Optical
Multi-chamber Suction Sampler
Oxygen Sensor
Oxygen Sensor Spot
Pressure Vessel
Pump
ROV SuBastian
instrument
BCO-DMO Standard Instruments
FKt230629
service
Deployment Activity
East Pacific Rise 9°N 104°W
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The Underworld of Hydrothermal Vents
https://schmidtocean.org/cruise/underworld-of-hydrothermal-vents/
The Underworld of Hydrothermal Vents
<p>Since the discovery of deep-sea hydrothermal vents in 1977, scientists realize that life exists above and below the seafloor. The extent to which they are interconnected, however, remains poorly understood. We propose to characterize subsurface biosphere diversity, from viruses to animals, at deep-sea hydrothermal vents to elucidate the nature and extent of connectivity between the surficial and subsurface biospheres. We will test the hypotheses that 1) eukaryotic life is also an integral component of the subseafloor biosphere; 2) the subseafloor is inhabited by both cosmopolitan and endemic protists and fungi; and 3) the subseafloor habitats harbor larvae -and perhaps adult life stages- of vent endemic animals. This research will transform our understanding of the ecology/evolution of subseafloor habitats and our thinking about animal recruitment at deep-sea vents. Consistent with SOI's mission, we will expand our knowledge of limits of eukaryotic life. During a three-week cruise with R/V Falkor and ROV SuBastian to "East Pacific Rise" vents at the 9º50'N region, we will use in situ and lab experiments, molecular identification with amplicon sequencing, Illumina NextSeq 500 technology, 2bRAD population genetic analyses, and RT qPCR for functional analyses. Our data management plan includes deposition of metadata/samples to long-term repositories. Outreach includes inquiry-learning telepresence programs for children from USA and Europe.</p>
Vent Underworld
largerWorkCitation
project
eng; USA
biota
oceans
East Pacific Rise 9°N 104°W
-104.294
-104.29138
9.83846
9.98494
2023-07-07
2023-07-21
East Pacific Rise
0
BCO-DMO catalogue of parameters from Copepod Pressure Vessel Experiments from samples collected from the East Pacific Rise during ROV SuBastian deployments and R/V Falkor (too) cruise FKt230627 in June-July 2023
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/923000/data/download
download
onLine
dataset
<p>Specimens were collected with ROV SuBastian at 9°N 50’N and 104°W along the&nbsp;East Pacific Rise in the Tica vent area and sites Bio9 and Biovent (~2500 m depth) during expedition FKt230627 in July 2023 aboard R/V Falkor(too).&nbsp;</p>
<p>Deep-sea hydrothermal vent copepods were sampled from two different habitats: diffuse flow habitats dominated by&nbsp;<em>Riftia pachyptila</em>&nbsp;tubeworms and&nbsp;<em>Bathymodiolus thermophilus</em>&nbsp;mussels, and focused flow habitats dominated by&nbsp;<em>Alvinella pompejana</em>&nbsp;pompeii worms. A suction sampler and/or ROV manipulator grabs were used to sample detritus and megafauna respectively. These samples were then sorted for copepods and subsampled to be used for a total of 118 LT<sub>50</sub>/LD<sub>50</sub>&nbsp;exposure incubations performed on board the research vessel. Each incubation contained 3 replicate vials with 10 copepods each, for a total of 3540 copepods.</p>
<p>Diverse exposure times, temperatures, pressures, and oxic/anoxic conditions were selected to explore the tolerance of deep-sea hydrothermal vent copepods in relation to habitat, temperature, oxygen concentration and pressure.&nbsp;Exposure times were the following: 2h, 4h, 8h, 10h. Temperature ranges for each incubation were established based on the ROV’s temperature probe readings. A control temperature of 4°C was always included to represent similar thermal conditions to the ambient bottom water around hydrothermal vents. The other temperatures (12, 22, 25, 28, 31, 34, 37, 40, 43°C) were selected to obtain a complete survival curve that ranged from ~100% survival at the lowest temperature to ~0% at the highest temperature for each habitat/time/oxygen/pressure treatment combination. Two oxygen concentration conditions were tested: oxic (163.88 ± 5.82 µM before and 95.63 ± 9.14 µM after incubations) and anoxic (0.24 ± 0.11 µM before and 1.76 ± 0.38 µM after incubations). Concentrations were monitored before and after incubations using self-adhesive Oxygen Sensor Spots mounted on the inside of the incubation vials (SP-PSt3-SA) and a fiber-optic oxygen meter (Fibox 4).</p>
<p>Ten live copepods were transferred using a pipette to each seawater-filled 5.9 ml glass vial&nbsp;(Exetainer 719W) equipped with an airtight pressure relief cap. The glass vials were placed into pressure vessels and pressurized up to 200 bar (3000 psi) using a Waters 515 HPLC Pump, and then placed in a water bath (ARGOLAB WB 12 Lt) to keep a constant temperature. At the end of each incubation the vessel was depressurized, and the content of each vial was examined under a Leica EZ4 W stereomicroscope to record the number of living/dead copepods. After the experiment, dead copepod specimens were not disposed, but instead were fixed in 95% Ethanol for preservation.</p>
<p>The data was used to estimate the median lethal temperature (LD<sub>50</sub>) corresponding to each exposure time (LT<sub>50</sub>) by fitting a survival curve to the temperature and respective copepod survival data using Nonlinear Least Squares regression and isolating the theoretical 50% survival temperature.</p>
Specified by the Principal Investigator(s)
<p>At the end of each incubation the vessel was depressurized, and the content of each vial was examined under a stereomicroscope to record the number of living/dead copepods. Data include the counts of living/dead copepods per experiment.&nbsp;The data was used to estimate the median lethal temperature (LD<sub>50</sub>) corresponding to each exposure time (LT<sub>50</sub>) by fitting a survival curve to the temperature and respective copepod survival data using Nonlinear Least Squares regression and isolating the theoretical 50% survival temperature.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
ARGOLAB WB 12 Lt
ARGOLAB WB 12 Lt
PI Supplied Instrument Name: ARGOLAB WB 12 Lt PI Supplied Instrument Description:Glass vials were placed into pressure vessels and placed in a water bath (ARGOLAB WB 12 Lt) to keep a constant temperature. Instrument Name: ARGOLAB Water Bath 12 Liters Instrument Short Name:ARGOLAB WB 12 Lt Instrument Description: ARGOLab waterbath without recirculation pump.
Working range: +5°C above ambient temperature to 100°C.
Tank capacity: 12 liters.
Electronic temperature regulator with integrated PID control
Visual and acoustic alarms that can be silenced by the operator.
Safety class 2 according to DIN 12880.
Temperature stability at 37 °C: ± 0.1 °C.
Temperature accuracy: ± 0.2 °C.
Digital timer with programming range 1 min - 99 h and 59 min, and continuous operation.
Rack capacity: up to 4 modules.
Tank dimensions (W x H x D): 300 x 240 x 200 mm
Weight: 12 Kg.
Power supply: 220 ± 10% V / 50-60 Hz, Power: 900 W.
Leica EZ4 W Compound Microscope
Leica EZ4 W Compound Microscope
PI Supplied Instrument Name: Leica EZ4 W Compound Microscope PI Supplied Instrument Description:At the end of each incubation the content of each vial was examined under a stereomicroscope to record the number of living/dead copepods. Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
ROV SuBastian Multi-Chamber Suction Sampler
ROV SuBastian Multi-Chamber Suction Sampler
PI Supplied Instrument Name: ROV SuBastian Multi-Chamber Suction Sampler PI Supplied Instrument Description:Specimens were collected with ROV SuBastian suction sampler at the vent sites. Instrument Name: Multi-chamber Suction Sampler Instrument Short Name:'slurp' sampler Instrument Description: An underwater device that collects animals and other samples under gentle suction and deposits them into a collection chamber. Also known as an oceanographic 'slurp' sampler. The primary components are an electrical or hydraulic pump, outlet hose connected to collection chamber, and inlet hose.
Fibox 4 stand-alone fiber optic oxygen meter
Fibox 4 stand-alone fiber optic oxygen meter
PI Supplied Instrument Name: Fibox 4 stand-alone fiber optic oxygen meter PI Supplied Instrument Description:Oxygen levels were measured with a Fibox 4 fiber optic oxygen meter to monitor whether the samples were oxic or anoxic. Instrument Name: Oxygen Sensor Instrument Short Name:Dissolved Oxygen Sensor Instrument Description: An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed
Self-adhesive Oxygen Sensor Spot SP-PSt3-SA
Self-adhesive Oxygen Sensor Spot SP-PSt3-SA
PI Supplied Instrument Name: Self-adhesive Oxygen Sensor Spot SP-PSt3-SA PI Supplied Instrument Description:(*** Need informaiton from the submitter to be put in here about how they were used...***) Instrument Name: Oxygen Sensor Spot Instrument Short Name:Oxygen Sensor Patch Instrument Description: Oxygen sensor spots allow oxygen measurements in closed sample containers with contactless read-out through a transparent window (glass, acryl glass) with special adapters and optical fibers. These patches often have a self-adhesive option.
customized pressure vessel
customized pressure vessel
PI Supplied Instrument Name: customized pressure vessel PI Supplied Instrument Description:Pressure vessels are custom items (see Klose et al. 2015; www.pnas.org/cgi/doi/10.1073/pnas.1501160112) Instrument Name: Pressure Vessel Instrument Short Name:pressurized container Instrument Description: A pressure vessel is a container designed to hold gases or liquids at a pressure substantially different from the ambient pressure. Construction methods and materials may be chosen to suit the pressure application, and will depend on the size of the vessel, the contents, working pressure, mass constraints, and the number of items required. Examples include glassware, autoclaves, compressed gas cylinders, compressors (including refrigeration), vacuum chambers and custom designed laboratory vessels.
Waters 515 HPLC Pump
Waters 515 HPLC Pump
PI Supplied Instrument Name: Waters 515 HPLC Pump PI Supplied Instrument Description:Glass vials were placed into pressure vessels and pressurized using a Waters 515 HPLC Pump up to 200 bar (3000 psi). Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps
ROV SuBastian
ROV SuBastian
PI Supplied Instrument Name: ROV SuBastian PI Supplied Instrument Description:Specimens were collected with ROV SuBastian at the vent sites Tica, Biovent and Bio9 using suction sampler and/or manipulator grabs. Instrument Name: ROV SuBastian Instrument Short Name: Instrument Description: ROV SuBastian is operated from the research vessel Falkor and the R/V Falkor(too). The ROV is outfitted with a suite of sensors and scientific equipment to support scientific data and sample collection, as well as interactive research, experimentation, and technology development. More information available at https://schmidtocean.org/technology/robotic-platforms/4500-m-remotely-operated-vehicle-rov/
Cruise: FKt230629
FKt230629
R/V Falkor (too)
Community Standard Description
International Council for the Exploration of the Sea
R/V Falkor (too)
vessel
FKt230629
Monika Bright
University of Vienna
https://service.rvdata.us/data/cruise/FKt230629/doc/FKt230629_expedition_report.pdf
Report describing FKt230629
R/V Falkor (too)
Community Standard Description
International Council for the Exploration of the Sea
R/V Falkor (too)
vessel