http://lod.bco-dmo.org/id/dataset/923159
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-03-22
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Semi-quantitative cell proteome of marine Synechococcus WH8102 using DIA-MS, interactive nutrient-temperature responses in stable chemostat bioreactors from laboratory experiments conducted in 2019 at the University of California, Irvine
2025-09-11
publication
2025-09-11
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-09-12
publication
https://doi.org/10.26008/1912/bco-dmo.923159.1
Adam Martiny
University of California-Irvine
principalInvestigator
Mak A. Saito
Woods Hole Oceanographic Institution
principalInvestigator
Nathan S. Garcia
University of California-Irvine
principalInvestigator
Matthew R. McIlvin
Woods Hole Oceanographic Institution
principalInvestigator
Dawn Moran
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Garcia, N. S., McIlvin, M. R., Moran, D., Saito, M. A., Martiny, A. (2025) Semi-quantitative cell proteome of marine Synechococcus WH8102 using DIA-MS, interactive nutrient-temperature responses in stable chemostat bioreactors from laboratory experiments conducted in 2019 at the University of California, Irvine. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-09-11 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.923159.1 [access date]
Synechococcus WH8102 nutrient-temperature chemostats Dataset Description: Methods and Sampling: <p><em>Experimental design</em></p>
<p><em>Synechococcus</em>&nbsp;cultures (WH8102) were grown in polycarbonate bottles&nbsp;with a continuous chemostat method used previously (Garcia et al. 2016)&nbsp;in artificial seawater. We used two concentration ratios of macronutrients (NO<sub>3</sub><sup>-</sup>:PO<sub>4</sub><sup>3-</sup>&nbsp;= 1.7 and 80) and 3 levels of temperature (20, 24 and 28°C) with a slow dilution rate (0.18 d<sup>-1</sup>) to ensure treatment-wise culture stability.&nbsp;Target concentrations of NO3- and PO43- in chemostats were 45 µM and 0.56 µM, respectively in the N:P=80 treatment, and 15.9 µM and 9.2 µM, respectively in the N:P=1.7 treatment.&nbsp;White light was supplied at 125 µmol quanta m<sup>-2</sup>&nbsp;s<sup>-1</sup>&nbsp;on a 12h:12h light:dark cycle. Chemostat equilibria were monitored by measuring culture cell density and forward scatter (<em>FSC<sub>H</sub></em>) with a Novocyte flow cytometer 1000 (Acea Biosciences, Inc, San Diego, CA). Cells for proteome analysis were collected after an acclimation period on days 38, 43, 47, 50 and 57&nbsp;with a 47 mm polycarbonate filter (0.2 µm pore size) 7-8 hours into the light period, pelleted by centrifugation (21,130 g for 3 minutes), flash frozen in liquid nitrogen, and stored at -80°C.</p>
<p><em>Protein extraction and peptide preparation</em></p>
<p>Proteins were extracted by heating pelleted cells at 95°C for 10 min and gently shaking at room temperature for 30 min in a buffer solution (400 µL – 1760 µL; 50 mM HEPES pH 8.5 (Boston BioProducts #BB-2082), 1% SDS in HPLC grade water) before centrifuging at 14100 g for 20 min at room temperature and removing the supernatant. Sodium dodecyl sulfate (1%) is a strong detergent for diverse matrices including cell membranes (Hughes et al. 2014).&nbsp;Benzonase nuclease (50 units; Novagen #70746-3) was added to 400 µL extracted protein sample and incubated at 37°C for 30 min. Samples were reduced by adding 20 µL of 200 mM DTT (Fisher #BP172-5) in 50 mM HEPES pH 8.5 at 45°C for 30 min and alkylated with 40 µL of 400 mM iodoacetamide (Acros #122270050) in HEPES pH 8.5 for 30 min at 24°C. The reaction was quenched by adding 40 µL of 200 mM DTT in 50 mM HEPES pH 8.5.&nbsp;SpeedBead Magnetic Carboxylate Modified Particles (GE Healthcare #65152105050250 and #45152105050250) were prepared according to Hughes et al. (2014) and added (20 µg/µL) to 400 µL of extracted protein sample.&nbsp;Samples were incubated with formic acid (pH of 2-3) and washed with ethanol and acetonitrile using a magnetic rack. Protein was measured with the BCA method (Thermo Scientific Micro BCA Protein Assay Kit #23235) and digested overnight at 37°C with 1 part trypsin (Promega #V5280; dissolved in HEPES pH 8.0, 0.5 µg/µL) and 25 parts protein. Peptides were washed with acetonitrile and ethanol using a magnetic rack and diluted to a target concentration of 0.1% trifluoroacetic acid or 1% formic acid and a final concentration of 1 µg/µL.</p>
<p><em>Mass spectrometry of peptides</em></p>
<p>Similar to other analyses&nbsp;(Searle et al. 2018), peptides were analyzed using a Michrom Advance HPLC system coupled to a Q-Exactive mass spectrometer (Thermo Scientific instrument version 2.8) with a Michrom Advance CaptiveSpray source, using the constant injection concentration of 1&nbsp;µg/µL (one microgram per microliter)&nbsp;to allow uniformity across the dataset. Samples were concentrated onto a C18 column&nbsp;(Reprosil-Gold, Dr. Maisch GmbH) and eluted in a non-linear, 200-min gradient of formic acid and acetonitrile buffers. Full MS1 scans were performed (35,000 resolution, 3e6 AGC target, 60 ms maximum IT, 385 to 1015 m/z) with overlapping DIA scans (17,500 resolution, 1e6 AGC target, 60 ms maximum IT, 24.0 m/z isolation windows, normalized collision energy of 27, loop count 25).&nbsp;</p>
<p><em>Proteomic data analysis</em></p>
<p>Data-independent acquisition mass spectrometry sample data were analyzed using Scaffold DIA (2.2.1), converted to mzML format (ProteoWizard 3.0.11748, Chambers et al (2012)), and individually searched against Syn8102_uniprot-proteome_UP000001422.fasta with a peptide and fragment mass tolerance of 10.0 ppm. Percolator (3.01) filtered peptides for a maximum false discovery rate of 0.01. Charged peptides (2-3) with length (6-30) were considered. EncyclopeDIA (0.9.6) selected the 5 highest quality fragment ions for quantitation (Searle et al. 2018).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1848576 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1848576
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2135035 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2135035
Funding provided by NSF Division of Integrative Organismal Systems (NSF IOS) Award Number: IOS-2137339 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2137339
completed
Adam Martiny
University of California-Irvine
949-824-9713
Earth System Science & Ecology and Evolutionary Biology 3208 Croul Hall
Irvine
CA
92697
USA
amartiny@uci.edu
pointOfContact
Mak A. Saito
Woods Hole Oceanographic Institution
508-289-2393
266 Woods Hole Rd. MS #51 Watson Lab
Woods Hole
MA
02543
USA
msaito@whoi.edu
pointOfContact
Nathan S. Garcia
University of California-Irvine
Irvine
CA
USA
n8garcia@gmail.com
pointOfContact
Matthew R. McIlvin
Woods Hole Oceanographic Institution
Watson 222A, Mail Stop: 52
Woods Hole
MA
02543
USA
mmcilvin@whoi.edu
pointOfContact
Dawn Moran
Woods Hole Oceanographic Institution
dmoran@whoi.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Protein_ID_long_form
Protein_ID_short_form
Gene_name1
Gene_name2
Protein_name
Molecular_Weight
Peptide_Count
Sample_Number
Temp_chemostat
Nutrient_chemostat
Treatment_replicate
Chemostat_day
Norm_peak_area
Michrom Advance HPLC system
Q-Exactive mass spectrometer
8 L polycarbonate bottles
theme
None, User defined
protein_ID
sample description
molecular weight
count
incubation or cultivation temperature (not ambient water temp)
No BCO-DMO term
replicate
time_elapsed
featureType
BCO-DMO Standard Parameters
High-Performance Liquid Chromatograph
Mass Spectrometer
no_bcodmo_term
no_bcodmo_term
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Convergence: RAISE: Linking the adaptive dynamics of plankton with emergent global ocean biogeochemistry
https://osprey.bco-dmo.org/project/764270
Convergence: RAISE: Linking the adaptive dynamics of plankton with emergent global ocean biogeochemistry
<p><em>NSF Award Abstract:</em><br />
Due to their sheer abundance and high activity, microorganisms have the potential to greatly influence how ecosystems are affected by changes in their environment. However, descriptions of microbial physiology and diversity are local and highly complex and thus rarely considered in Earth System Models. Thus, the researchers focus on a convergence research framework that can qualitatively and quantitatively integrate eco-evolutionary changes in microorganisms with global biogeochemistry. Here, the investigators will develop an approach that integrates the knowledge and tools of biologists, mathematicians, engineers, and geoscientists to understand the link between the ocean nutrient and carbon cycles. The integration of data and knowledge from diverse fields will provide a robust, biologically rich, and computationally efficient prediction for the variation in plankton resource requirements and the biogeochemical implications, addressing a fundamental challenge in ocean science. In addition, the project can serve as a road map for many other research groups facing a similar lack of convergence between biology and geoscience.</p>
<p>Traditionally, the cellular elemental ratios of Carbon, Nitrogen, and Phosphorus (C:N:P) of marine communities have been considered static at Redfield proportions but recent studies have demonstrated strong latitudinal variation. Such regional variation may have large - but poorly constrained - implications for marine biodiversity, biogeochemical functioning, and atmospheric carbon dioxide levels. As such, variations in ocean community C:N:P may represent an important biological feedback. Here, the investigators propose a convergence research framework integrating cellular and ecological processes controlling microbial resource allocations with an Earth System model. The approach combines culture experiments and omics measurements to provide a molecular understanding of cellular resource allocations. Using a mathematical framework of increasing complexity describing communicating, moving demes, the team will quantify the extent to which local mixing, environmental heterogeneity and evolution lead to systematic deviations in plankton resource allocations and C:N:P. Optimization tools from engineering science will be used to facilitate the quantitative integration of models and observations across a range of scales and complexity levels. Finally, global ocean modeling will enable understanding of how plankton resource use impacts Earth System processes. By integrating data and knowledge across fields, scales and complexity, the investigators will develop a robust link between variation in plankton C:N:P and global biogeochemical cycles.</p>
Ocean Stoichiometry
largerWorkCitation
project
Collaborative Research: The stoichiometric trait distribution of the marine microbiome
https://osprey.bco-dmo.org/project/893402
Collaborative Research: The stoichiometric trait distribution of the marine microbiome
<p><em>NSF Award Abstract:</em><br />
The elemental ratios of carbon, nitrogen, and phosphorus (C:N:P) have been considered fixed proportions in marine environments but recent work has demonstrated changes across latitudes. Such variation can have large implications for marine biogeochemistry and atmospheric CO_2 levels. As such, future variations in ocean community C:N:P could be a key feedback to global change. The elemental composition of particulate organic matter (POM) represents the aggregate value of diverse microorganisms as well as non-living particles. However, we currently do not understand how individual cells and particles contribute to observed variation in the C:N:P of POM. This project is determining the biomass C:N:P of individual microbial cells grown under a range of conditions and sampled from diverse ocean biomes. Because different individuals are likely to have different fates (e.g., loss to sinking, lysis, predation), understanding how the trait distribution of microbial biomass C:N:P relates to cell size and trophic mode and how environmental conditions affect each trait’s distribution offers new perspectives and insight into marine C, N, and P cycles. This project supports two PhD students and multiple undergraduate students. The PhD students are integrated into existing networks on microbiome science at each institution with opportunities to collaborate across diverse disciplines. Undergraduate students at both institutions are being recruited through existing training programs that target underrepresented groups. In addition, PI Hall is part of a collaborative of Ecologists and Poets at CSU, that look at ways to translate ecological relationships to non-traditional media to make it more accessible and impactful to the general public. This group is exploring the nature of individuality within the marine microbiome by creating trait distributions of written text and removing different modes of individuals (e.g., words) to better understand and communicate how individuals from the smallest organisms on the planet (marine microorganisms) can have large effects on the surrounding ecosystem (i.e., the planet). Results from this project are being incorporated into future projects of the working group at Colorado State University including public presentations, art installations, and published materials.</p>
<p>The aim of this project is to quantify the relationship between environmental conditions and marine microbial C:N:P by assessing the individuality in microbial elemental stoichiometry. To achieve this the project uses energy dispersive spectroscopy (EDS) to assess the stoichiometric trait distribution of populations and communities under different resource and temperature treatments and oceanographic field work across a broad latitudinal gradient. The researchers hypothesize that the relationship between the stoichiometry of particulate organic matter (POM) and environmental conditions are masked by distinct responses of individual constituents of the marine microbiome. They hypothesize that these distinct responses result in a multi-modal distribution of particulate carbon (C), nitrogen (N), and phosphorus (P) of the marine microbiome. The investigators are characterizing the distribution of three stoichiometric traits (biomass C:N, C:P, and N:P) for 50 marine isolates (both autotrophs and heterotrophs) under different resource and temperature environments. They are characterizing the same trait distribution for marine communities sampled from different regions of the ocean for the same resource and temperature environments as the population experiments. They are participating on an ocean going cruise to characterize stoichiometric trait distribution of the marine microbiome across natural gradients of resources and temperature. Understanding how constituent members of microbial communities alter their biomass in response to environmental change is providing a missing link between the variation in the ocean’s environment and particulate C:N:P ratios for diverse marine environments.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
StoichTraitD
largerWorkCitation
project
eng; USA
oceans
2019-01-01
2019-12-31
Indian Ocean; Laboratory Ecophysiology
0
BCO-DMO catalogue of parameters from Semi-quantitative cell proteome of marine Synechococcus WH8102 using DIA-MS, interactive nutrient-temperature responses in stable chemostat bioreactors from laboratory experiments conducted in 2019 at the University of California, Irvine
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/983964.rdf
Name: Protein_ID_long_form
Units: unitless
Description: <p>This is the protein ID (long form) (this is the same as the Raw protein ID)</p>
http://lod.bco-dmo.org/id/dataset-parameter/983965.rdf
Name: Protein_ID_short_form
Units: unitless
Description: <p>This is the protein ID (short form)</p>
http://lod.bco-dmo.org/id/dataset-parameter/983966.rdf
Name: Gene_name1
Units: unitless
Description: <p>Gene name 1</p>
http://lod.bco-dmo.org/id/dataset-parameter/983967.rdf
Name: Gene_name2
Units: unitless
Description: <p>Gene name 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/983968.rdf
Name: Protein_name
Units: unitless
Description: <p>Protein name (e.g. "C-phycoerythrin class 2 subunit alpha")</p>
http://lod.bco-dmo.org/id/dataset-parameter/983969.rdf
Name: Molecular_Weight
Units: kilodalton (kDa)
Description: <p>Molecular Weight of protein</p>
http://lod.bco-dmo.org/id/dataset-parameter/983970.rdf
Name: Peptide_Count
Units: unitless
Description: <p>This is the number of peptides that are identified by the mass-spec method and subsequently used to identify the protein.</p>
http://lod.bco-dmo.org/id/dataset-parameter/983971.rdf
Name: Sample_Number
Units: unitless
Description: <p>Sample number (1_through_30)</p>
http://lod.bco-dmo.org/id/dataset-parameter/983972.rdf
Name: Temp_chemostat
Units: Degrees Celsius
Description: <p>Temperature of chemostat</p>
http://lod.bco-dmo.org/id/dataset-parameter/983973.rdf
Name: Nutrient_chemostat
Units: unitless
Description: <p>Nitrate:phosphate (NO3_to_PO4) input ratio into chemostats</p>
http://lod.bco-dmo.org/id/dataset-parameter/983974.rdf
Name: Treatment_replicate
Units: unitless
Description: <p>Treatment replicate (1_through_5)</p>
http://lod.bco-dmo.org/id/dataset-parameter/983975.rdf
Name: Chemostat_day
Units: days
Description: <p>Sampling day from chemostat start day</p>
http://lod.bco-dmo.org/id/dataset-parameter/983976.rdf
Name: Norm_peak_area
Units: unitless
Description: <p>Normalized peak areas (exclusive intensity). [relative values, unitless]</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
7584544
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/636d705a-4a1c-4f71-9b1a-9c9cdb2888c4/content
download
https://doi.org/10.26008/1912/bco-dmo.923159.1
download
onLine
dataset
<p><em>Experimental design</em></p>
<p><em>Synechococcus</em>&nbsp;cultures (WH8102) were grown in polycarbonate bottles&nbsp;with a continuous chemostat method used previously (Garcia et al. 2016)&nbsp;in artificial seawater. We used two concentration ratios of macronutrients (NO<sub>3</sub><sup>-</sup>:PO<sub>4</sub><sup>3-</sup>&nbsp;= 1.7 and 80) and 3 levels of temperature (20, 24 and 28°C) with a slow dilution rate (0.18 d<sup>-1</sup>) to ensure treatment-wise culture stability.&nbsp;Target concentrations of NO3- and PO43- in chemostats were 45 µM and 0.56 µM, respectively in the N:P=80 treatment, and 15.9 µM and 9.2 µM, respectively in the N:P=1.7 treatment.&nbsp;White light was supplied at 125 µmol quanta m<sup>-2</sup>&nbsp;s<sup>-1</sup>&nbsp;on a 12h:12h light:dark cycle. Chemostat equilibria were monitored by measuring culture cell density and forward scatter (<em>FSC<sub>H</sub></em>) with a Novocyte flow cytometer 1000 (Acea Biosciences, Inc, San Diego, CA). Cells for proteome analysis were collected after an acclimation period on days 38, 43, 47, 50 and 57&nbsp;with a 47 mm polycarbonate filter (0.2 µm pore size) 7-8 hours into the light period, pelleted by centrifugation (21,130 g for 3 minutes), flash frozen in liquid nitrogen, and stored at -80°C.</p>
<p><em>Protein extraction and peptide preparation</em></p>
<p>Proteins were extracted by heating pelleted cells at 95°C for 10 min and gently shaking at room temperature for 30 min in a buffer solution (400 µL – 1760 µL; 50 mM HEPES pH 8.5 (Boston BioProducts #BB-2082), 1% SDS in HPLC grade water) before centrifuging at 14100 g for 20 min at room temperature and removing the supernatant. Sodium dodecyl sulfate (1%) is a strong detergent for diverse matrices including cell membranes (Hughes et al. 2014).&nbsp;Benzonase nuclease (50 units; Novagen #70746-3) was added to 400 µL extracted protein sample and incubated at 37°C for 30 min. Samples were reduced by adding 20 µL of 200 mM DTT (Fisher #BP172-5) in 50 mM HEPES pH 8.5 at 45°C for 30 min and alkylated with 40 µL of 400 mM iodoacetamide (Acros #122270050) in HEPES pH 8.5 for 30 min at 24°C. The reaction was quenched by adding 40 µL of 200 mM DTT in 50 mM HEPES pH 8.5.&nbsp;SpeedBead Magnetic Carboxylate Modified Particles (GE Healthcare #65152105050250 and #45152105050250) were prepared according to Hughes et al. (2014) and added (20 µg/µL) to 400 µL of extracted protein sample.&nbsp;Samples were incubated with formic acid (pH of 2-3) and washed with ethanol and acetonitrile using a magnetic rack. Protein was measured with the BCA method (Thermo Scientific Micro BCA Protein Assay Kit #23235) and digested overnight at 37°C with 1 part trypsin (Promega #V5280; dissolved in HEPES pH 8.0, 0.5 µg/µL) and 25 parts protein. Peptides were washed with acetonitrile and ethanol using a magnetic rack and diluted to a target concentration of 0.1% trifluoroacetic acid or 1% formic acid and a final concentration of 1 µg/µL.</p>
<p><em>Mass spectrometry of peptides</em></p>
<p>Similar to other analyses&nbsp;(Searle et al. 2018), peptides were analyzed using a Michrom Advance HPLC system coupled to a Q-Exactive mass spectrometer (Thermo Scientific instrument version 2.8) with a Michrom Advance CaptiveSpray source, using the constant injection concentration of 1&nbsp;µg/µL (one microgram per microliter)&nbsp;to allow uniformity across the dataset. Samples were concentrated onto a C18 column&nbsp;(Reprosil-Gold, Dr. Maisch GmbH) and eluted in a non-linear, 200-min gradient of formic acid and acetonitrile buffers. Full MS1 scans were performed (35,000 resolution, 3e6 AGC target, 60 ms maximum IT, 385 to 1015 m/z) with overlapping DIA scans (17,500 resolution, 1e6 AGC target, 60 ms maximum IT, 24.0 m/z isolation windows, normalized collision energy of 27, loop count 25).&nbsp;</p>
<p><em>Proteomic data analysis</em></p>
<p>Data-independent acquisition mass spectrometry sample data were analyzed using Scaffold DIA (2.2.1), converted to mzML format (ProteoWizard 3.0.11748, Chambers et al (2012)), and individually searched against Syn8102_uniprot-proteome_UP000001422.fasta with a peptide and fragment mass tolerance of 10.0 ppm. Percolator (3.01) filtered peptides for a maximum false discovery rate of 0.01. Charged peptides (2-3) with length (6-30) were considered. EncyclopeDIA (0.9.6) selected the 5 highest quality fragment ions for quantitation (Searle et al. 2018).</p>
Specified by the Principal Investigator(s)
<p>Data-independent acquisition mass spectrometry&nbsp;sample data were analyzed using Scaffold DIA (2.2.1), converted to mzML format (ProteoWizard 3.0.11748, Chambers et al (2012)), and individually searched against Syn8102_uniprot-proteome_UP000001422.fasta with a peptide and fragment mass tolerance of 10.0 ppm. Percolator (3.01) filtered peptides for a maximum false discovery rate of 0.01. Charged peptides (2-3) with length (6-30) were considered. EncyclopeDIA (0.9.6) selected the 5 highest quality fragment ions for quantitation&nbsp;(Searle et al. 2018).<br />
<br />
.raw proteomics files are&nbsp;accessible at the PRIDE Archive (https://www.ebi.ac.uk/pride/archive/projects/PXD043180).</p>
Specified by the Principal Investigator(s)
Dataset version 1:
Wide format table: "923159_v1_norm-peak-area_exclusive-intensity-wide-format.xlsx" history:
* This contains the wide format of the dataset (separate normalized peak area columns per Protein_ID_(long_form),Temp_chemostat,Nutrient_chemostat,Treatment_replicate,Chemostat_day). It is the original Excel file provided to BCO-DMO on Sept 11th, 2025.
* This format contains 5 header lines containing sample and treatment metadata values. The data rows start as row 6.
* The last row contains no values except ("#VALUE!" as gene name 1 and protein name.)
Long format table: "923159_v1_norm-peak-area_exclusive-intensity.csv" history:
* This table is in "long" format with one column for Normalized peak area, and treatment/sample metadata contained in additional columns: "Sample_Number,Temp_chemostat,Nutrient_chemostat,Treatment_replicate,Chemostat_day."
* Sheet 1 of submitted file "Normalized peak areas (exclusive intensity for BCO-DMO).xlsx" (submitted Apr 2nd, 2024) was modified to add a row of sample ID numbers (1-30) before import into the bco-dmo data system, discussed with the file submitter. The last row was not imported, it contained no values except ("#VALUE!" as gene name 1 and protein name.)
* The wide to long transformation was performed with an unpivot operation.
* Values "NaN" were imported as missing data values.
** Missing data values are displayed differently based on the file format you download. They are blank in csv files, "NaN" in MatLab files, etc.
* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores. Can not start with a number]
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Michrom Advance HPLC system
Michrom Advance HPLC system
PI Supplied Instrument Name: Michrom Advance HPLC system PI Supplied Instrument Description:HPLC system and mass spectrometer: A Michrom Advance HPLC system was coupled to a Q-Exactive mass spectrometer (Thermo Scientific instrument version 2.8) with a Michrom Advance CaptiveSpray source. Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/
Q-Exactive mass spectrometer
Q-Exactive mass spectrometer
PI Supplied Instrument Name: Q-Exactive mass spectrometer PI Supplied Instrument Description:HPLC system and mass spectrometer: A Michrom Advance HPLC system was coupled to a Q-Exactive mass spectrometer (Thermo Scientific instrument version 2.8) with a Michrom Advance CaptiveSpray source. Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
8 L polycarbonate bottles
8 L polycarbonate bottles
PI Supplied Instrument Name: 8 L polycarbonate bottles PI Supplied Instrument Description:Synechococcus cultures (WH8102) were grown in polycarbonate bottles. Instrument Name: no_bcodmo_term Instrument Short Name: Instrument Description: No relevant match in BCO-DMO instrument vocabulary. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/999/
PI Supplied Instrument Name: PI Supplied Instrument Description:47 mm polycarbonate filter (0.2 µm pore size, Millipore): Cells for proteome analysis were collected with a 47 mm polycarbonate filter (0.2 µm pore size, Millipore) 7-8 hours into the light period, pelleted by centrifugation in 2 mL centrifuge tubes (21,130 g for 3 minutes), flash frozen in liquid nitrogen, and stored at -80°C. Instrument Name: no_bcodmo_term Instrument Short Name: Instrument Description: No relevant match in BCO-DMO instrument vocabulary. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/999/