http://lod.bco-dmo.org/id/dataset/923908
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-03-28
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Population fitness measurements collected for Acartia tonsa during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA)
2024-03-28
publication
2024-03-28
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-04-05
publication
https://doi.org/10.26008/1912/bco-dmo.923908.1
Hans G. Dam
University of Connecticut
principalInvestigator
Hannes Baumann
University of Connecticut
principalInvestigator
Michael Finiguerra
University of Connecticut
principalInvestigator
Melissa Pespeni
University of Vermont
principalInvestigator
Reid Brennan
University of Vermont
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Dam, H. G., Baumann, H., Finiguerra, M., Pespeni, M., Brennan, R. (2024) Population fitness measurements collected for Acartia tonsa during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-03-28 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.923908.1 [access date]
Ocean warming & acidification experiment: Acartia tonsa population fitness Dataset Description: Methods and Sampling: <p>Copepods were collected in June of 2016 from Esker Point Beach in Groton, Connecticut, USA (41.320725°N, 72.001643°W) and raised for at least three generations as stock cultures prior to the start of transgenerational experiments to limit maternal effects (Falconer, 1989. <em>Introduction to Quantitative Genetics</em>). Stock cultures were split evenly into eight groups of 160 females and 80 males. Four of these eight groups were acclimatized to high temperature at 1 degree Celsius per day and used to seed the two high temperature treatments (OW and OWA). The other four groups remained at ambient temperature and were used to seed the ambient and acidification treatments. After temperature acclimatization, groups of stock cultures seeded the parental (F0) individuals for two days. Stock culture groups yielded an average of 7,173 eggs per group to produce approximately 57,000 parental (F0) eggs. Resulting parental eggs and N1 nauplii were acclimated to one of four experimental treatments over the entire F0 generation. The four lines of the copepods were established with four replicates of each condition. The target (actual ± standard deviation) conditions were: ambient temperature = 18 degrees Celsius (°C) (18 ± 0.34, N = 330), ambient pCO2 = 400 μatm (379 ± 36, N = 18; pH = 8.26 ± 0.1, N = 330); high temperature = 22°C (22 ± 0.81, N = 336), and high pCO2 = 2000 µatm (2301 ± 215, N = 18; pH = 7.55 ± 0.08, N = 330). Copepods were fed every 48-72 hours at food-replete concentrations (≥800 micrograms (μg) Carbon per liter (L)) consisting of equal proportions of the phytoplankters <em>Tetraselmis</em> sp., <em>Rhodomonas</em> sp., and <em>Thalassiosira weissflogii</em>, deliberately raised under ambient conditions for the entire length of the experiment to avoid confounding effects of possible changes in food quality due to the different temperature and CO2 among treatments.</p>
<p>The population net reproductive rate, λ, was calculated as the dominant eigenvalue of an assembled projected age-structured Leslie matrix constructed from survival and fecundity data (Caswell, H. 2001. <em>Matrix Population Models: Construction, Analysis, and Interpretation</em>). Briefly, day-specific probabilities of survival are calculated from day-specific survival as Px = l<sub>x</sub> /(l<sub>x</sub>−1) where l<sub>x</sub> represents the proportion of individuals on day <em>x</em> and l<sub>x</sub> - 1 represents the proportion of individuals on day <em>x − 1</em>. Probabilities of survival on day 1 are assumed to be 100%, or a value of 1.0. EPR was calculated as (E<sub>u</sub>+E<sub>h</sub>)/t where E<sub>u </sub>represents unhatched eggs, E<sub>h</sub> represents hatched eggs (nauplii) and t represents egg-laying time. HS was calculated as E<sub>h</sub>/(E<sub>u</sub>+E<sub>h</sub>). Fecundity rates equal the product of EPR and HS. Because only females produce offspring, total fecundity rates must be scaled to the sex ratio (proportion of females to males). To account for differences in individual development time for each treatment, fecundity rates are assigned to all days after the first matured adult is observed. We assume that surviving individuals represented by the survival experiments are equally as likely to experience any of the fecundity values observed in EPR experiments. Therefore, each mate-pair fecundity rate was paired with each survival beaker to construct a matrix. This yields a maximum of 120 matrices per treatment per generation (3 survival beakers × 4 replicate cultures × 10 mate pairs). Relative measures of each value are calculated as the trait value divided by the mean value of that trait. Standardized measures of each value are calculated as the trait value minus the mean trait value and divided by the standard deviation. The target (actual ± standard deviation) conditions were as follows: ambient (AM) temperature = 18 °C (18 ± 0.34, N = 330), AM pCO2= 400 μatm (379 ± 36, N = 18; pH = 8.26 ± 0.1, N = 330); high temperature = 22 °C (22 ± 0.81, N = 336); and high pCO2= 2,000 µatm (2,301 ± 215, N = 18; pH = 7.55 ± 0.08, N = 330). AM target levels represented extant conditions for this species in northeast Atlantic estuaries. Full methods can be found in Dam, et al. 2021 Nature Climate Change. doi: 1<a href="http://dx.doi.org/0.1038/s41558-021-01131-5" target="_blank">0.1038/s41558-021-01131-5</a>.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1559075 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1559075
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1559180 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1559180
completed
Hans G. Dam
University of Connecticut
860-405-9098
Dept. of Marine Sciences 1080 Shennecossett Road
Groton
CT
06340-6048
USA
hans.dam@uconn.edu
pointOfContact
Hannes Baumann
University of Connecticut
860-405-9297
Department of Marine Sciences 1080 Shennecossett Road
Groton
CT
06340-6048
USA
hannes.baumann@uconn.edu
pointOfContact
Michael Finiguerra
University of Connecticut
860-405-9082
Department of Marine Sciences
Groton
CT
06340-6048
USA
michael.finiguerra@uconn.edu
pointOfContact
Melissa Pespeni
University of Vermont
802-656-0628
109 Carrigan Dr. Marsh Life Sciences 120A
Burlington
VT
05405
US
melissa.pespeni@uvm.edu
pointOfContact
Reid Brennan
University of Vermont
reid.brennan@gmail.com
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Generation
Treatment
Rep
lambda
surv
epr
hf
sex
dev_time
lambda_stand
surv_stand
epr_stand
hf_stand
sex_stand
dev_stand
lambda_rel
surv_rel
epr_rel
hf_rel
sex_rel
dev_rel
theme
None, User defined
sample description
treatment
replicate
reproduction rate
survival probability
fecundity
ratio
development time
featureType
BCO-DMO Standard Parameters
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Response of marine copepods to warming temperature and ocean acidification
https://osprey.bco-dmo.org/project/706023
Collaborative Research: Response of marine copepods to warming temperature and ocean acidification
<p><strong><em>NSF Award Abstract:</em></strong><br />
Over time, our oceans are becoming both warmer and higher dissolved carbon dioxide. The latter condition is called ocean acidification. The consequences of these simultaneous changes for populations of marine organisms are not well understood. For this project, the investigators will conduct a series of laboratory experiments to determine how two closely-related, common species of Acartia copepods will respond to the interactive effects of warming and acidification and also how well these species can adapt over multiple generations to changing ocean conditions. Since these copepods are key species in coastal food webs, results will have important implications for understanding and predicting how marine ecosystems may respond to future climate change. The investigators will share results from the research through traditional print media, case studies, and video mini lectures. The goal will be for educators of all levels to easily access material on climate change and ocean acidification to include in teaching curricula, in alignment with recommendations for universal design for learning. The project is a collaborative effort between an established professor at the University of Connecticut and an early-career female scientist at the University of Vermont. It will provide training and opportunities for collaborative, interdisciplinary research for two postdoctoral investigators, two graduate students and an undergraduate student.</p>
<p>The project's main goals are: 1) to test the simultaneous effects of temperature and carbon dioxide under current and future conditions on life history traits throughout the life cycle for two key copepod species, warm-adapted Acartia tonsa and cold-adapted Acartia hudsonica; 2) to test for adaptive capacity of both copepod species to a warmer and carbon-dioxide-enriched ocean; 3) to measure the genetic and maternally-induced changes across multiple generations of experimental selection in future conditions in both copepod species, and to identify the genes and pathways responding to selection. The investigators will use experiments encompassing current and projected temperature and carbon-dioxide conditions, will determine the roles of each variable and their interaction on traits that affect the fitness of both copepod species. They will also determine which life stages are most sensitive to individual or simultaneous stress conditions. Through multigenerational selection experiments, the investigators will identify and characterize the mechanisms of copepod evolutionary adaptation. Finally, they will measure genomic changes across the generations under all four experimental conditions to quantify the relative contributions of genetic and maternally-induced change in the physiological and life history traits of copepods in response to near-future climate conditions.</p>
Copepod Response to Warming Temp and OA
largerWorkCitation
project
eng; USA
oceans
-72.001643
-72.001643
41.320725
41.320725
2017-07-02
2018-08-15
North western Atlantic ocean; Gulf of Maine, coastal and estuarine habitats
0
BCO-DMO catalogue of parameters from Population fitness measurements collected for Acartia tonsa during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/923936.rdf
Name: Generation
Units: unitless
Description: <p>The generation at which fitness was estimated</p>
http://lod.bco-dmo.org/id/dataset-parameter/923937.rdf
Name: Treatment
Units: unitless
Description: <p>The treatment that the orgnaisms were evaluated in, and where traits were measured and fitness was estimated. 1 = ambient (AM): temperature = 18 °C, pCO2= 400 µatm. 2 = ocean acidification (OA): temperature = 18 °C, pCO2= 2000 µatm. 3 = ocean warming (OW): temperature = 22 °C, pCO2= 400 µatm. 4 = ocean warming and acidification (OWA): temperature = 22 °C, pCO2= 2,000 µatm.</p>
http://lod.bco-dmo.org/id/dataset-parameter/923938.rdf
Name: Rep
Units: unitless
Description: <p>The biological replicate associated with the population fitness estimates</p>
http://lod.bco-dmo.org/id/dataset-parameter/923939.rdf
Name: lambda
Units: per generation
Description: <p>The net reproductive rate estimates</p>
http://lod.bco-dmo.org/id/dataset-parameter/923940.rdf
Name: surv
Units: unitless
Description: <p>The corresponding survival probabilities</p>
http://lod.bco-dmo.org/id/dataset-parameter/923941.rdf
Name: epr
Units: eggs per female per day
Description: <p>The corresponding egg production rate measurements</p>
http://lod.bco-dmo.org/id/dataset-parameter/923942.rdf
Name: hf
Units: nauplii per number of eggs laid
Description: <p>The corresponding hatching success rates</p>
http://lod.bco-dmo.org/id/dataset-parameter/923943.rdf
Name: sex
Units: females per total copepods
Description: <p>The corresponding sex ratio</p>
http://lod.bco-dmo.org/id/dataset-parameter/923944.rdf
Name: dev_time
Units: days to adulthood
Description: <p>The corresponding development time</p>
http://lod.bco-dmo.org/id/dataset-parameter/923945.rdf
Name: lambda_stand
Units: unitless
Description: <p>The standardized lambda value</p>
http://lod.bco-dmo.org/id/dataset-parameter/923946.rdf
Name: surv_stand
Units: unitless
Description: <p>The standardized survival value</p>
http://lod.bco-dmo.org/id/dataset-parameter/923947.rdf
Name: epr_stand
Units: unitless
Description: <p>The standardized egg production rate (epr) value</p>
http://lod.bco-dmo.org/id/dataset-parameter/923948.rdf
Name: hf_stand
Units: unitless
Description: <p>The standardized hatching success</p>
http://lod.bco-dmo.org/id/dataset-parameter/923949.rdf
Name: sex_stand
Units: unitless
Description: <p>The standardized sex ratio (proportion of females to males)</p>
http://lod.bco-dmo.org/id/dataset-parameter/923950.rdf
Name: dev_stand
Units: unitless
Description: <p>The standardized development time</p>
http://lod.bco-dmo.org/id/dataset-parameter/923951.rdf
Name: lambda_rel
Units: unitless
Description: <p>The relative lambda value</p>
http://lod.bco-dmo.org/id/dataset-parameter/923952.rdf
Name: surv_rel
Units: unitless
Description: <p>The relative survival value</p>
http://lod.bco-dmo.org/id/dataset-parameter/923953.rdf
Name: epr_rel
Units: unitless
Description: <p>The relative epr value</p>
http://lod.bco-dmo.org/id/dataset-parameter/923954.rdf
Name: hf_rel
Units: unitless
Description: <p>The relative hatching success</p>
http://lod.bco-dmo.org/id/dataset-parameter/923955.rdf
Name: sex_rel
Units: unitless
Description: <p>The relative sex ratio</p>
http://lod.bco-dmo.org/id/dataset-parameter/923956.rdf
Name: dev_rel
Units: unitless
Description: <p>The relative development time</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
575983
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/ced7cea5-fe85-4fdf-abc5-01f12e8b5399/content
download
https://doi.org/10.26008/1912/bco-dmo.923908.1
download
onLine
dataset
<p>Copepods were collected in June of 2016 from Esker Point Beach in Groton, Connecticut, USA (41.320725°N, 72.001643°W) and raised for at least three generations as stock cultures prior to the start of transgenerational experiments to limit maternal effects (Falconer, 1989. <em>Introduction to Quantitative Genetics</em>). Stock cultures were split evenly into eight groups of 160 females and 80 males. Four of these eight groups were acclimatized to high temperature at 1 degree Celsius per day and used to seed the two high temperature treatments (OW and OWA). The other four groups remained at ambient temperature and were used to seed the ambient and acidification treatments. After temperature acclimatization, groups of stock cultures seeded the parental (F0) individuals for two days. Stock culture groups yielded an average of 7,173 eggs per group to produce approximately 57,000 parental (F0) eggs. Resulting parental eggs and N1 nauplii were acclimated to one of four experimental treatments over the entire F0 generation. The four lines of the copepods were established with four replicates of each condition. The target (actual ± standard deviation) conditions were: ambient temperature = 18 degrees Celsius (°C) (18 ± 0.34, N = 330), ambient pCO2 = 400 μatm (379 ± 36, N = 18; pH = 8.26 ± 0.1, N = 330); high temperature = 22°C (22 ± 0.81, N = 336), and high pCO2 = 2000 µatm (2301 ± 215, N = 18; pH = 7.55 ± 0.08, N = 330). Copepods were fed every 48-72 hours at food-replete concentrations (≥800 micrograms (μg) Carbon per liter (L)) consisting of equal proportions of the phytoplankters <em>Tetraselmis</em> sp., <em>Rhodomonas</em> sp., and <em>Thalassiosira weissflogii</em>, deliberately raised under ambient conditions for the entire length of the experiment to avoid confounding effects of possible changes in food quality due to the different temperature and CO2 among treatments.</p>
<p>The population net reproductive rate, λ, was calculated as the dominant eigenvalue of an assembled projected age-structured Leslie matrix constructed from survival and fecundity data (Caswell, H. 2001. <em>Matrix Population Models: Construction, Analysis, and Interpretation</em>). Briefly, day-specific probabilities of survival are calculated from day-specific survival as Px = l<sub>x</sub> /(l<sub>x</sub>−1) where l<sub>x</sub> represents the proportion of individuals on day <em>x</em> and l<sub>x</sub> - 1 represents the proportion of individuals on day <em>x − 1</em>. Probabilities of survival on day 1 are assumed to be 100%, or a value of 1.0. EPR was calculated as (E<sub>u</sub>+E<sub>h</sub>)/t where E<sub>u </sub>represents unhatched eggs, E<sub>h</sub> represents hatched eggs (nauplii) and t represents egg-laying time. HS was calculated as E<sub>h</sub>/(E<sub>u</sub>+E<sub>h</sub>). Fecundity rates equal the product of EPR and HS. Because only females produce offspring, total fecundity rates must be scaled to the sex ratio (proportion of females to males). To account for differences in individual development time for each treatment, fecundity rates are assigned to all days after the first matured adult is observed. We assume that surviving individuals represented by the survival experiments are equally as likely to experience any of the fecundity values observed in EPR experiments. Therefore, each mate-pair fecundity rate was paired with each survival beaker to construct a matrix. This yields a maximum of 120 matrices per treatment per generation (3 survival beakers × 4 replicate cultures × 10 mate pairs). Relative measures of each value are calculated as the trait value divided by the mean value of that trait. Standardized measures of each value are calculated as the trait value minus the mean trait value and divided by the standard deviation. The target (actual ± standard deviation) conditions were as follows: ambient (AM) temperature = 18 °C (18 ± 0.34, N = 330), AM pCO2= 400 μatm (379 ± 36, N = 18; pH = 8.26 ± 0.1, N = 330); high temperature = 22 °C (22 ± 0.81, N = 336); and high pCO2= 2,000 µatm (2,301 ± 215, N = 18; pH = 7.55 ± 0.08, N = 330). AM target levels represented extant conditions for this species in northeast Atlantic estuaries. Full methods can be found in Dam, et al. 2021 Nature Climate Change. doi: 1<a href="http://dx.doi.org/0.1038/s41558-021-01131-5" target="_blank">0.1038/s41558-021-01131-5</a>.</p>
Specified by the Principal Investigator(s)
<p>Data were processed and analyzed with R (v 4.0.2). Code for data analysis and visualization is located in Zenodo at: <a href="https://doi.org/10.5281/zenodo.5115103" target="_blank">https://doi.org/10.5281/zenodo.5115103</a>.</p>
Specified by the Principal Investigator(s)
- Imported original file "lambda_results_devtime_surv_epr_hf_sex_standardized_relative.txt" into the BCO-DMO system.
- Renamed fields to comply with BCO-DMO naming conventions.
- Saved final file as "923908_v1_a_tonsa_population_fitness.csv".
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact