http://lod.bco-dmo.org/id/dataset/924126
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-04-01
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Egg production rate (EPR) and egg hatching success (HS) data for Acartia tonsa during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA)
2024-04-02
publication
2024-04-02
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-04-05
publication
https://doi.org/10.26008/1912/bco-dmo.924126.1
Hans G. Dam
University of Connecticut
principalInvestigator
Hannes Baumann
University of Connecticut
principalInvestigator
Michael Finiguerra
University of Connecticut
principalInvestigator
Melissa Pespeni
University of Vermont
principalInvestigator
Reid Brennan
University of Vermont
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Dam, H. G., Baumann, H., Finiguerra, M., Pespeni, M., Brennan, R. (2024) Egg production rate (EPR) and egg hatching success (HS) data for Acartia tonsa during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-04-02 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.924126.1 [access date]
Ocean warming & acidification experiment: Acartia hudsonica Egg production and hatching success Dataset Description: Methods and Sampling: <p>Three hundred copepods were collected in April of 2018 from eastern Long Island Sound, Connecticut, USA (41.3°N, 72.0°W) and raised for one year (~12 generations) (14 degrees Celsius (°C), 400 microatmospheres (μatm) CO2, 30 ‰ salinity, 12:12 hours light:dark) as stock cultures to limit maternal effects (Falconer, 1989, <em>Introduction to Quantitative Genetics</em>). Three resulting stock cultures with &gt;2,000 individuals each were combined and then split evenly into three groups for each of the four treatments. Groups were acclimatized within a generation to temperature (15°C or 13°C, 1°C per day) and pCO2 (1000 μatm, 100 μatm per day, OA treatments only). Groups seeded the F0 individuals for 7-10 days yielding ~15,000 eggs per treatment. Resulting F0 eggs and nauplii were combined for each treatment, redistributed among three replicate cultures, and returned to their respective experimental conditions. The experimental environmental conditions were: 1) Ambient control (AM): 13°C, 400 µatm CO2, pH = 8.2; 2) Ocean Acidification (OA): 13°C, 1000 µatm CO2, pH = 7.85; 3) Ocean Warming (OW): 15°C, 400 μatm CO2, pH = 8.2; 4) Combined warming and acidification (OWA): 15°C, 1000 μatm CO2, pH = 7.85. Copepods were fed equal proportions of the live phytoplankters <em>Tetraselmis</em> sp., <em>Rhodomonas</em> sp., and T<em>halassiosira weissflogii</em> every 48-72 hours to achieve food-replete conditions (≥600 micrograms (μg) Carbon per liter (L)) (Feinberg and Dam, 1998. <em>Marine Ecology Progress Series</em>), deliberately raised under ambient conditions to avoid confounding effects of possible food quality changes.</p>
<p>For each replicate culture within a treatment, 12 pairs of newly developed adult males and females were placed into 25 milliliter (mL) Petri dishes housed in the plexiglass enclosure described above for 96 hours (N= 108 per treatment for F0 to F4). Adults were transferred to a new dish after 48 hours. For food limitation experiments in F11, the 12 pairs were split equally among the three food concentrations. Adults in food limitation experiments laid eggs for 72 hours and were transferred to a new dish daily to maintain food concentration during the experiment. After the egg-laying period, adults were checked for survival and removed from the Petri dishes. Eggs were left in the dishes for an additional 72 hours to hatch and then preserved with non-acid Lugol’s solution. Dishes with dead males were used for EPR, but not HS, since fertilization could not be assumed. Dishes with dead females were discarded. We independently evaluated survival in additional assays, thus the measurements for this assay were only used to estimate the number of offspring produced for live copepods. EPR was calculated as the number of eggs produced per female per day and HS was calculated as the proportion of live nauplii from produced eggs.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1559075 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1559075
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1559180 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1559180
completed
Hans G. Dam
University of Connecticut
860-405-9098
Dept. of Marine Sciences 1080 Shennecossett Road
Groton
CT
06340-6048
USA
hans.dam@uconn.edu
pointOfContact
Hannes Baumann
University of Connecticut
860-405-9297
Department of Marine Sciences 1080 Shennecossett Road
Groton
CT
06340-6048
USA
hannes.baumann@uconn.edu
pointOfContact
Michael Finiguerra
University of Connecticut
860-405-9082
Department of Marine Sciences
Groton
CT
06340-6048
USA
michael.finiguerra@uconn.edu
pointOfContact
Melissa Pespeni
University of Vermont
802-656-0628
109 Carrigan Dr. Marsh Life Sciences 120A
Burlington
VT
05405
US
melissa.pespeni@uvm.edu
pointOfContact
Reid Brennan
University of Vermont
reid.brennan@gmail.com
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Generation
Number
Treatment
Temp
pH
EPRtot
Hftot
Generation_c
Rep
Olympus SZH-ILLD Stereoscope
theme
None, User defined
sample description
replicate
treatment
fecundity
featureType
BCO-DMO Standard Parameters
Microscope - Optical
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Response of marine copepods to warming temperature and ocean acidification
https://osprey.bco-dmo.org/project/706023
Collaborative Research: Response of marine copepods to warming temperature and ocean acidification
<p><strong><em>NSF Award Abstract:</em></strong><br />
Over time, our oceans are becoming both warmer and higher dissolved carbon dioxide. The latter condition is called ocean acidification. The consequences of these simultaneous changes for populations of marine organisms are not well understood. For this project, the investigators will conduct a series of laboratory experiments to determine how two closely-related, common species of Acartia copepods will respond to the interactive effects of warming and acidification and also how well these species can adapt over multiple generations to changing ocean conditions. Since these copepods are key species in coastal food webs, results will have important implications for understanding and predicting how marine ecosystems may respond to future climate change. The investigators will share results from the research through traditional print media, case studies, and video mini lectures. The goal will be for educators of all levels to easily access material on climate change and ocean acidification to include in teaching curricula, in alignment with recommendations for universal design for learning. The project is a collaborative effort between an established professor at the University of Connecticut and an early-career female scientist at the University of Vermont. It will provide training and opportunities for collaborative, interdisciplinary research for two postdoctoral investigators, two graduate students and an undergraduate student.</p>
<p>The project's main goals are: 1) to test the simultaneous effects of temperature and carbon dioxide under current and future conditions on life history traits throughout the life cycle for two key copepod species, warm-adapted Acartia tonsa and cold-adapted Acartia hudsonica; 2) to test for adaptive capacity of both copepod species to a warmer and carbon-dioxide-enriched ocean; 3) to measure the genetic and maternally-induced changes across multiple generations of experimental selection in future conditions in both copepod species, and to identify the genes and pathways responding to selection. The investigators will use experiments encompassing current and projected temperature and carbon-dioxide conditions, will determine the roles of each variable and their interaction on traits that affect the fitness of both copepod species. They will also determine which life stages are most sensitive to individual or simultaneous stress conditions. Through multigenerational selection experiments, the investigators will identify and characterize the mechanisms of copepod evolutionary adaptation. Finally, they will measure genomic changes across the generations under all four experimental conditions to quantify the relative contributions of genetic and maternally-induced change in the physiological and life history traits of copepods in response to near-future climate conditions.</p>
Copepod Response to Warming Temp and OA
largerWorkCitation
project
eng; USA
oceans
-72
-72
41.3
41.3
2017-07-02
2018-08-15
North western Atlantic ocean; Gulf of Maine, coastal and estuarine habitats
0
BCO-DMO catalogue of parameters from Egg production rate (EPR) and egg hatching success (HS) data for Acartia tonsa during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/924197.rdf
Name: Generation
Units: unitless
Description: <p>The corresponding generation when egg production and egg hatching success was measured</p>
http://lod.bco-dmo.org/id/dataset-parameter/924198.rdf
Name: Number
Units: unitless
Description: <p>The individual technical replicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/924199.rdf
Name: Treatment
Units: unitless
Description: <p>Corresponding treatment evaluated</p>
http://lod.bco-dmo.org/id/dataset-parameter/924200.rdf
Name: Temp
Units: degrees Celsius
Description: <p>Target temperature for each treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/924201.rdf
Name: pH
Units: pH units
Description: <p>The target pH for each treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/924202.rdf
Name: EPRtot
Units: eggs per female per day
Description: <p>The rate of eggs produced</p>
http://lod.bco-dmo.org/id/dataset-parameter/924203.rdf
Name: Hftot
Units: nauplii per egg laid
Description: <p>The frequency of hatching success</p>
http://lod.bco-dmo.org/id/dataset-parameter/924204.rdf
Name: Generation_c
Units: unitless
Description: <p>A numeric column for the corresponding generation when egg production and egg hatching success was measured; equivalent to the "Generation" column</p>
http://lod.bco-dmo.org/id/dataset-parameter/924205.rdf
Name: Rep
Units: unitless
Description: <p>The biological culture replicate for each treatment</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
11735
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/926d4b83-927e-41ed-a998-a2ea41247268/content
download
https://doi.org/10.26008/1912/bco-dmo.924126.1
download
onLine
dataset
<p>Three hundred copepods were collected in April of 2018 from eastern Long Island Sound, Connecticut, USA (41.3°N, 72.0°W) and raised for one year (~12 generations) (14 degrees Celsius (°C), 400 microatmospheres (μatm) CO2, 30 ‰ salinity, 12:12 hours light:dark) as stock cultures to limit maternal effects (Falconer, 1989, <em>Introduction to Quantitative Genetics</em>). Three resulting stock cultures with &gt;2,000 individuals each were combined and then split evenly into three groups for each of the four treatments. Groups were acclimatized within a generation to temperature (15°C or 13°C, 1°C per day) and pCO2 (1000 μatm, 100 μatm per day, OA treatments only). Groups seeded the F0 individuals for 7-10 days yielding ~15,000 eggs per treatment. Resulting F0 eggs and nauplii were combined for each treatment, redistributed among three replicate cultures, and returned to their respective experimental conditions. The experimental environmental conditions were: 1) Ambient control (AM): 13°C, 400 µatm CO2, pH = 8.2; 2) Ocean Acidification (OA): 13°C, 1000 µatm CO2, pH = 7.85; 3) Ocean Warming (OW): 15°C, 400 μatm CO2, pH = 8.2; 4) Combined warming and acidification (OWA): 15°C, 1000 μatm CO2, pH = 7.85. Copepods were fed equal proportions of the live phytoplankters <em>Tetraselmis</em> sp., <em>Rhodomonas</em> sp., and T<em>halassiosira weissflogii</em> every 48-72 hours to achieve food-replete conditions (≥600 micrograms (μg) Carbon per liter (L)) (Feinberg and Dam, 1998. <em>Marine Ecology Progress Series</em>), deliberately raised under ambient conditions to avoid confounding effects of possible food quality changes.</p>
<p>For each replicate culture within a treatment, 12 pairs of newly developed adult males and females were placed into 25 milliliter (mL) Petri dishes housed in the plexiglass enclosure described above for 96 hours (N= 108 per treatment for F0 to F4). Adults were transferred to a new dish after 48 hours. For food limitation experiments in F11, the 12 pairs were split equally among the three food concentrations. Adults in food limitation experiments laid eggs for 72 hours and were transferred to a new dish daily to maintain food concentration during the experiment. After the egg-laying period, adults were checked for survival and removed from the Petri dishes. Eggs were left in the dishes for an additional 72 hours to hatch and then preserved with non-acid Lugol’s solution. Dishes with dead males were used for EPR, but not HS, since fertilization could not be assumed. Dishes with dead females were discarded. We independently evaluated survival in additional assays, thus the measurements for this assay were only used to estimate the number of offspring produced for live copepods. EPR was calculated as the number of eggs produced per female per day and HS was calculated as the proportion of live nauplii from produced eggs.</p>
Specified by the Principal Investigator(s)
<p>All data were processed in R (v 4.0.2). Data analysis and visualization code can be accessed at: <a href="https://zenodo.org/badge/latestdoi/505896789" target="_blank">https://zenodo.org/badge/latestdoi/505896789</a>.</p>
Specified by the Principal Investigator(s)
- Imported original file "EPR_HS_data_total_w_11.txt" into the BCO-DMO system.
- Renamed fields to comply with BCO-DMO naming conventions.
- Saved final file as "924126_v1_a_hudsonica_ep_and_hs.csv".
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Olympus SZH-ILLD Stereoscope
Olympus SZH-ILLD Stereoscope
PI Supplied Instrument Name: Olympus SZH-ILLD Stereoscope Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/