http://lod.bco-dmo.org/id/dataset/926311
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-04-30
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Synechococcus batch culture data (cell quotas and ratios (C,N,P), size, and diameter) from laboratory experiments in 2021 to 2022 with related isolates cultured across a range of temperatures (16-25C)
2024-04-30
publication
2024-04-30
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-05-02
publication
https://doi.org/10.26008/1912/bco-dmo.926311.1
Adam Martiny
University of California-Irvine
principalInvestigator
Nathan S. Garcia
University of California-Irvine
principalInvestigator
Renne Harcourt
University of California-Irvine
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Harcourt, R., Garcia, N. S., Martiny, A. (2024) Synechococcus batch culture data (cell quotas and ratios (C,N,P), size, and diameter) from laboratory experiments in 2021 to 2022 with related isolates cultured across a range of temperatures (16-25C). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-04-30 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.926311.1 [access date]
Dataset Description: <p>These data were published in the results publication Harcourt R, Garcia NS, Martiny AC (2024).&nbsp;&nbsp;The data table for this dataset was derived from table S8 in&nbsp;Harcourt R, Garcia NS, Martiny AC (2024), with additional culture information (sample date, lat, lon, site location) from the Roscoff Culture Collection (RCC), Station Biologique de Roscoff.&nbsp; See&nbsp;Harcourt R, Garcia NS, Martiny AC (2024) for additional related supplemental figures and tables.</p> Methods and Sampling: <p><!--[if gte mso 9]><xml>
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<p>We incubated serially transferred cultures of <em>Synechococcus </em>(CC9311, BL107, ROS8604, CC9902, representing two clades) in triplicate 1 L flasks at 16°C, 18°C, 20°C, 22°C, 25°C, and 27°C. We supplied ambient light (60 µmol quanta m<sup>-2</sup> s<sup>-1</sup>) using white fluorescent lamps on a 12:12 light-dark cycle. We grew cultures in modified artificial seawater media. To ascertain that we did not impose growth rates upon cultures and the observed growth rates are a product of strain and environmental conditions, we used nutrient replete media and sampled prior to cultures encountering nutrient limitation;<strong> </strong>we supplied nitrate (NO<sub>3</sub><sup>-</sup>) and phosphate (PO<sub>4</sub><sup>3−</sup>) in concentrations of 125 µM and 10 µM, respectively. Cells utilized a mean of 17% of the supplied nitrogen, and 22% of the supplied phosphorus, indicating cultures did not reach nutrient limitation. We transferred media and diluted cultures using an open flame in a hood in order to avoid contamination. We diluted weekly by approximately an order of magnitude to avoid nutrient limitation and maintain a stable growth rate<strong>.</strong></p>
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<p>We measured particulate organic carbon (POC), particulate organic nitrogen (PON), and particulate organic phosphorus (POP), as well as cell enumeration by flow cytometry, following the methods outlined in Garcia et al. (2016). We sampled after seven doublings or one month to acclimate cells to the temperature conditions. We vacuum filtered POC, PON (150 mL), and POP (50 mL) samples onto pre-combusted GF/F Whatman glass filters (450°C) at 10 psi. We dried particulate organic carbon and particulate organic nitrogen samples at 50-80°C for a minimum of 48 hours and pelletized prior to analysis using a Flash EA 1112 NC Soil Analyzer (Thermo-Scientific, MA). We rinsed filtered particulate organic phosphorus samples with 0.17 M NaSO<sub>4</sub>, immersed the filter in 2 mL of MgSO<sub>4</sub>, dried at 80°C overnight, and combusted at 450°C for 2 hours. We then added 5 mL of 0.2 M HCl and baked the samples at 80-90°C. We measured particulate organic phosphorus samples via colorimetric assay following the Bermuda Atlantic Time-series methodology using a Genesys 10S UV-vis spectrophotometer (Thermo-Scientific) at 885 nm.</p>
<p>We measured culture cell density every two-three days and immediately prior to sampling using a NovoCyte 1000 flow cytometer (excitation laser 488 nm, emission peak 575 nm) and forward scatter. We assessed growth rate using flow cytometry based on increases in biomass measured across time points using the following equation: <em>µ</em> = ln(<em>CD2</em>)-ln(<em>CD1</em>))/(<em>T2</em>-<em>T1</em>), in which <em>CD2</em> and <em>CD1</em> are cell counts in cells/mL<sup>-1</sup> on the most recent count and the previous count, respectively, and <em>T2</em> and <em>T1</em> represent time points. We accounted for recent dilutions in accounting for growth rate; we counted cells prior to and after dilutions to ensure growth rate was accurately assessed. We counted the cells at a flow rate of 35 µL/min. To assess heterotrophic populations, we stained the cultures with SYBR Green (Thermo-Fisher) for 15 minutes at room temperature, vortexed them, and counted using the (excitation laser 488 nm, emission peak 520 nm) channel. We recorded duplicate cell counts at each sampling.</p>
<p>We measured cell diameter by microscopy under oil immersion at 1000x magnification using the Axioplan2 and AxioView 1.4.5 sizing software (Carl Zeiss, Goettingen, Germany) with reference to a staged micrometer (Ted Pella Inc., Redding, CA). To estimate cell diameter, we created a conversion factor determined by plotting the mean observed cell diameters and mean forward scatter values for several strains of <em>Synechococcus</em>.</p>
<p><strong>Locations:</strong>&nbsp;Laboratory batch cultures in incubators from 16-25°C.&nbsp;The original isolation locations for the&nbsp;Synechococcus strains used were:<br />
31.91, -124.17, Pacific Ocean, California Current<br />
32.87, -117.26, Pacific Ocean, California Current<br />
41.72, 3.55, Mediterranean Sea, Balearic Sea<br />
48.72, -3.98, Atlantic Ocean, Britanny coast</p>
<p><strong>Organism Identifier (LifeSciences Identifier, LSID):</strong><br />
Synechococcus, urn:lsid:marinespecies.org:taxname:160572<br />
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Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2134950 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2134950
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2135035 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2135035
completed
Adam Martiny
University of California-Irvine
949-824-9713
Earth System Science & Ecology and Evolutionary Biology 3208 Croul Hall
Irvine
CA
92697
USA
amartiny@uci.edu
pointOfContact
Nathan S. Garcia
University of California-Irvine
Irvine
CA
USA
n8garcia@gmail.com
pointOfContact
Renne Harcourt
University of California-Irvine
rhcourt@uci.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Rep
Temp
Strain
Clade
GR
QN
QP
QC
FSCH
CN
NP
CP
Cell_Diameter_um
Grams_N
Grams_P
Grams_C
isolation_sample_date
isolation_lat
isolation_lon
isolation_depth
isolation_location
isolation_site
isolation_ecosystem
RCC_Number
RCC_Link
Flash EA 1112 NC Soil Analyzer (Thermo-Scientific, MA)
NovoCyte 1000 (Agilent, Santa Clara, CA)
Axioplan2 microscope (Carl Zeiss, Goettingen, Germany)
Genesys 10S UV-vis spectrophotometer (Thermo-Scientific)
theme
None, User defined
replicate
water temperature at measurement depth
sample description
growth
Nitrogen
Phosphorus
Elemental carbon (C)
No BCO-DMO term
Carbon to Nitrogen ratio (C:N)
Nitrogen to phosphorus ratio (N:P)
Carbon to Phosphorus ratio (C:P)
cell_size
date
latitude
longitude
depth
site description
external_link
featureType
BCO-DMO Standard Parameters
Elemental Analyzer
Flow Cytometer
Microscope - Optical
Spectrophotometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: The stoichiometric trait distribution of the marine microbiome
https://osprey.bco-dmo.org/project/893402
Collaborative Research: The stoichiometric trait distribution of the marine microbiome
<p>NSF abstract:<br />
The elemental ratios of carbon, nitrogen, and phosphorus (C:N:P) have been considered fixed proportions in marine environments but recent work has demonstrated changes across latitudes. Such variation can have large implications for marine biogeochemistry and atmospheric CO_2 levels. As such, future variations in ocean community C:N:P could be a key feedback to global change. The elemental composition of particulate organic matter (POM) represents the aggregate value of diverse microorganisms as well as non-living particles. However, we currently do not understand how individual cells and particles contribute to observed variation in the C:N:P of POM. This project is determining the biomass C:N:P of individual microbial cells grown under a range of conditions and sampled from diverse ocean biomes. Because different individuals are likely to have different fates (e.g., loss to sinking, lysis, predation), understanding how the trait distribution of microbial biomass C:N:P relates to cell size and trophic mode and how environmental conditions affect each trait’s distribution offers new perspectives and insight into marine C, N, and P cycles. This project supports two PhD students and multiple undergraduate students. The PhD students are integrated into existing networks on microbiome science at each institution with opportunities to collaborate across diverse disciplines. Undergraduate students at both institutions are being recruited through existing training programs that target underrepresented groups. In addition, PI Hall is part of a collaborative of Ecologists and Poets at CSU, that look at ways to translate ecological relationships to non-traditional media to make it more accessible and impactful to the general public. This group is exploring the nature of individuality within the marine microbiome by creating trait distributions of written text and removing different modes of individuals (e.g., words) to better understand and communicate how individuals from the smallest organisms on the planet (marine microorganisms) can have large effects on the surrounding ecosystem (i.e., the planet). Results from this project are being incorporated into future projects of the working group at Colorado State University including public presentations, art installations, and published materials.</p>
<p>The aim of this project is to quantify the relationship between environmental conditions and marine microbial C:N:P by assessing the individuality in microbial elemental stoichiometry. To achieve this the project uses energy dispersive spectroscopy (EDS) to assess the stoichiometric trait distribution of populations and communities under different resource and temperature treatments and oceanographic field work across a broad latitudinal gradient. The researchers hypothesize that the relationship between the stoichiometry of particulate organic matter (POM) and environmental conditions are masked by distinct responses of individual constituents of the marine microbiome. They hypothesize that these distinct responses result in a multi-modal distribution of particulate carbon (C), nitrogen (N), and phosphorus (P) of the marine microbiome. The investigators are characterizing the distribution of three stoichiometric traits (biomass C:N, C:P, and N:P) for 50 marine isolates (both autotrophs and heterotrophs) under different resource and temperature environments. They are characterizing the same trait distribution for marine communities sampled from different regions of the ocean for the same resource and temperature environments as the population experiments. They are participating on an ocean going cruise to characterize stoichiometric trait distribution of the marine microbiome across natural gradients of resources and temperature. Understanding how constituent members of microbial communities alter their biomass in response to environmental change is providing a missing link between the variation in the ocean’s environment and particulate C:N:P ratios for diverse marine environments.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
StoichTraitD
largerWorkCitation
project
eng; USA
oceans
-124.17
3.55
31.91
48.72
2021-09-01
2022-01-01
Indian Ocean; Laboratory Ecophysiology
0
BCO-DMO catalogue of parameters from Synechococcus batch culture data (cell quotas and ratios (C,N,P), size, and diameter) from laboratory experiments in 2021 to 2022 with related isolates cultured across a range of temperatures (16-25C)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/926434.rdf
Name: Rep
Units: unitless
Description: <p>biological replicate.</p>
http://lod.bco-dmo.org/id/dataset-parameter/926435.rdf
Name: Temp
Units: degrees Celsius (degC)
Description: <p>Temperature.</p>
http://lod.bco-dmo.org/id/dataset-parameter/926436.rdf
Name: Strain
Units: unitless
Description: <p>Strain of Synechococcus.</p>
http://lod.bco-dmo.org/id/dataset-parameter/926437.rdf
Name: Clade
Units: unitless
Description: <p>Clade of Synechococcus</p>
http://lod.bco-dmo.org/id/dataset-parameter/926438.rdf
Name: GR
Units: Growth rate per day (d-1)
Description: <p>Growth rate (<em>μ)</em> per day</p>
http://lod.bco-dmo.org/id/dataset-parameter/926439.rdf
Name: QN
Units: femtomoles (fmol)
Description: <p>Cell nitrogen quota</p>
http://lod.bco-dmo.org/id/dataset-parameter/926440.rdf
Name: QP
Units: femtomoles (fmol)
Description: <p>Cell phosphorus quota</p>
http://lod.bco-dmo.org/id/dataset-parameter/926441.rdf
Name: QC
Units: femtomoles (fmol)
Description: <p>Cell carbon quota</p>
http://lod.bco-dmo.org/id/dataset-parameter/926442.rdf
Name: FSCH
Units: unitless
Description: <p>Forward scatter height (cell size proxy).</p>
http://lod.bco-dmo.org/id/dataset-parameter/926443.rdf
Name: CN
Units: unitless
Description: <p>Carbon:nitrogen ratio.</p>
http://lod.bco-dmo.org/id/dataset-parameter/926444.rdf
Name: NP
Units: unitless
Description: <p>Nitrogen:phosphorus ratio</p>
http://lod.bco-dmo.org/id/dataset-parameter/926445.rdf
Name: CP
Units: unitless
Description: <p>Carbon:phosphorus ratio</p>
http://lod.bco-dmo.org/id/dataset-parameter/926446.rdf
Name: Cell_Diameter_um
Units: micrometers (um)
Description: <p>Cell diameter</p>
http://lod.bco-dmo.org/id/dataset-parameter/926447.rdf
Name: Grams_N
Units: grams (g)
Description: <p>Grams of nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/926448.rdf
Name: Grams_P
Units: grams (g)
Description: <p>Grams of phosphorus</p>
http://lod.bco-dmo.org/id/dataset-parameter/926449.rdf
Name: Grams_C
Units: grams (g)
Description: <p>Grams of carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/926450.rdf
Name: isolation_sample_date
Units: unitless
Description: <p>Sample date of strain isolation source.</p>
http://lod.bco-dmo.org/id/dataset-parameter/926451.rdf
Name: isolation_lat
Units: decimal degrees
Description: <p>Latitude of strain isolation source.</p>
http://lod.bco-dmo.org/id/dataset-parameter/926452.rdf
Name: isolation_lon
Units: decimal degrees
Description: <p>Longitude of strain isolation source.</p>
http://lod.bco-dmo.org/id/dataset-parameter/926453.rdf
Name: isolation_depth
Units: meters (m)
Description: <p>Depth of strain isolation source.</p>
http://lod.bco-dmo.org/id/dataset-parameter/926454.rdf
Name: isolation_location
Units: unitless
Description: <p>Location description (Sea or Ocean) for the strain isolation source.</p>
http://lod.bco-dmo.org/id/dataset-parameter/926455.rdf
Name: isolation_site
Units: unitless
Description: <p>Site description for stain isolation source.</p>
http://lod.bco-dmo.org/id/dataset-parameter/926456.rdf
Name: isolation_ecosystem
Units: unitless
Description: <p>Ecosystem (coastal, pelagic) of strain isolation source.</p>
http://lod.bco-dmo.org/id/dataset-parameter/926457.rdf
Name: RCC_Number
Units: unitless
Description: <p>Identifier in the Roscoff Culture Collection (RCC) for the strain.</p>
http://lod.bco-dmo.org/id/dataset-parameter/926458.rdf
Name: RCC_Link
Units: unitless
Description: <p>Link to the Roscoff Culture Collection (RCC) page for the strain.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
15990
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/30a259e4-b375-4c31-b17a-5bf9a6ff570f/content
download
https://doi.org/10.26008/1912/bco-dmo.926311.1
download
onLine
dataset
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<p>We incubated serially transferred cultures of <em>Synechococcus </em>(CC9311, BL107, ROS8604, CC9902, representing two clades) in triplicate 1 L flasks at 16°C, 18°C, 20°C, 22°C, 25°C, and 27°C. We supplied ambient light (60 µmol quanta m<sup>-2</sup> s<sup>-1</sup>) using white fluorescent lamps on a 12:12 light-dark cycle. We grew cultures in modified artificial seawater media. To ascertain that we did not impose growth rates upon cultures and the observed growth rates are a product of strain and environmental conditions, we used nutrient replete media and sampled prior to cultures encountering nutrient limitation;<strong> </strong>we supplied nitrate (NO<sub>3</sub><sup>-</sup>) and phosphate (PO<sub>4</sub><sup>3−</sup>) in concentrations of 125 µM and 10 µM, respectively. Cells utilized a mean of 17% of the supplied nitrogen, and 22% of the supplied phosphorus, indicating cultures did not reach nutrient limitation. We transferred media and diluted cultures using an open flame in a hood in order to avoid contamination. We diluted weekly by approximately an order of magnitude to avoid nutrient limitation and maintain a stable growth rate<strong>.</strong></p>
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<p>We measured particulate organic carbon (POC), particulate organic nitrogen (PON), and particulate organic phosphorus (POP), as well as cell enumeration by flow cytometry, following the methods outlined in Garcia et al. (2016). We sampled after seven doublings or one month to acclimate cells to the temperature conditions. We vacuum filtered POC, PON (150 mL), and POP (50 mL) samples onto pre-combusted GF/F Whatman glass filters (450°C) at 10 psi. We dried particulate organic carbon and particulate organic nitrogen samples at 50-80°C for a minimum of 48 hours and pelletized prior to analysis using a Flash EA 1112 NC Soil Analyzer (Thermo-Scientific, MA). We rinsed filtered particulate organic phosphorus samples with 0.17 M NaSO<sub>4</sub>, immersed the filter in 2 mL of MgSO<sub>4</sub>, dried at 80°C overnight, and combusted at 450°C for 2 hours. We then added 5 mL of 0.2 M HCl and baked the samples at 80-90°C. We measured particulate organic phosphorus samples via colorimetric assay following the Bermuda Atlantic Time-series methodology using a Genesys 10S UV-vis spectrophotometer (Thermo-Scientific) at 885 nm.</p>
<p>We measured culture cell density every two-three days and immediately prior to sampling using a NovoCyte 1000 flow cytometer (excitation laser 488 nm, emission peak 575 nm) and forward scatter. We assessed growth rate using flow cytometry based on increases in biomass measured across time points using the following equation: <em>µ</em> = ln(<em>CD2</em>)-ln(<em>CD1</em>))/(<em>T2</em>-<em>T1</em>), in which <em>CD2</em> and <em>CD1</em> are cell counts in cells/mL<sup>-1</sup> on the most recent count and the previous count, respectively, and <em>T2</em> and <em>T1</em> represent time points. We accounted for recent dilutions in accounting for growth rate; we counted cells prior to and after dilutions to ensure growth rate was accurately assessed. We counted the cells at a flow rate of 35 µL/min. To assess heterotrophic populations, we stained the cultures with SYBR Green (Thermo-Fisher) for 15 minutes at room temperature, vortexed them, and counted using the (excitation laser 488 nm, emission peak 520 nm) channel. We recorded duplicate cell counts at each sampling.</p>
<p>We measured cell diameter by microscopy under oil immersion at 1000x magnification using the Axioplan2 and AxioView 1.4.5 sizing software (Carl Zeiss, Goettingen, Germany) with reference to a staged micrometer (Ted Pella Inc., Redding, CA). To estimate cell diameter, we created a conversion factor determined by plotting the mean observed cell diameters and mean forward scatter values for several strains of <em>Synechococcus</em>.</p>
<p><strong>Locations:</strong>&nbsp;Laboratory batch cultures in incubators from 16-25°C.&nbsp;The original isolation locations for the&nbsp;Synechococcus strains used were:<br />
31.91, -124.17, Pacific Ocean, California Current<br />
32.87, -117.26, Pacific Ocean, California Current<br />
41.72, 3.55, Mediterranean Sea, Balearic Sea<br />
48.72, -3.98, Atlantic Ocean, Britanny coast</p>
<p><strong>Organism Identifier (LifeSciences Identifier, LSID):</strong><br />
Synechococcus, urn:lsid:marinespecies.org:taxname:160572<br />
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<p>We used the software NovoExpress 1.5.6 to count cells. We used NovoExpress 1.5.6 and AxioView 1.4.5 to determine cell size.<br />
&nbsp;</p>
Specified by the Principal Investigator(s)
* File "S8_TableRevised.csv" was imported into the BCO-DMO data system for this dataset. Values "N/A" imported as missing data values.
** Missing data values are displayed differently based on the file format you download. They are blank in csv files, "NaN" in MatLab files, etc.
* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores. Can not start with a number]
* Links to strain information in the Roscoff Culture Collection (RCC) that were included in a "Reference" column in the data table were corrected by obtaining the correct links in the corresponding results journal publication Harcourt, R., Garcia, N. S., & Martiny, A. C. (2024).
* Additional isolation sample collection information was added to the data table from the RCC collection metadata (sample date, lat, lon, additional site location descriptions, and identifier for the strain in the RCC collection).
* Date converted to ISO 8601 format
* "m" removed from depth values and instead included in column description metadata, depth column was then typed as numeric type.
* LSID added for Synecococcus in metadata text (from World Register of Marine Species)
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
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info@bco-dmo.org
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For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Flash EA 1112 NC Soil Analyzer (Thermo-Scientific, MA)
Flash EA 1112 NC Soil Analyzer (Thermo-Scientific, MA)
PI Supplied Instrument Name: Flash EA 1112 NC Soil Analyzer (Thermo-Scientific, MA) Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
NovoCyte 1000 (Agilent, Santa Clara, CA)
NovoCyte 1000 (Agilent, Santa Clara, CA)
PI Supplied Instrument Name: NovoCyte 1000 (Agilent, Santa Clara, CA) Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
Axioplan2 microscope (Carl Zeiss, Goettingen, Germany)
Axioplan2 microscope (Carl Zeiss, Goettingen, Germany)
PI Supplied Instrument Name: Axioplan2 microscope (Carl Zeiss, Goettingen, Germany) PI Supplied Instrument Description:Axioplan2 microscope (Carl Zeiss, Goettingen, Germany) and Stage micrometer (Ted Pella Inc., Redding, CA) Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
Genesys 10S UV-vis spectrophotometer (Thermo-Scientific)
Genesys 10S UV-vis spectrophotometer (Thermo-Scientific)
PI Supplied Instrument Name: Genesys 10S UV-vis spectrophotometer (Thermo-Scientific) Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/