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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/926959.rdf" xlink:actuate="onRequest">Suspended particle total mercury and monomethylmercury in the California Current Ecosystem (CCE) determined from samples collected on R/V Roger Revelle cruise RR2105 (P2107) in July to August 2021</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Cui, X., Adams, H. M., Schartup, A. T., Lamborg, C. (2024) Suspended particle total mercury and monomethylmercury in the California Current Ecosystem (CCE) determined from samples collected on R/V Roger Revelle cruise RR2105 (P2107) in July to August 2021. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-05-14 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.926959.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Suspended particle total mercury and monomethylmercury in the California Current Ecosystem (CCE) Dataset Description:  Methods and Sampling: &amp;lt;p&amp;gt;Suspended particles were collected using McLane Research in-situ pumps (WTS-LV) using modified, two-layer filter holders. The top filter holder layer was loaded with a 51-micrometer (μm) polyester mesh filter to collect large-size fraction particles (LSF). The other filter holder layer was loaded with a pre-combusted GF/F glass microfiber filter (Whatman) to collect small-size fraction particles (SSF, 1-51 μm). Filter holders were acid-leached before use, and GF/F filters were additionally combusted at 450 degrees Celsius (°C) for 4 hours. Sinking particles were collected by sediment traps.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Total mercury (THg) and monomethylmercury (MMHg) in suspended and sinking particles were determined by digesting filter portions in nitric acid (2N, trace mental grade, Fisher) for 4 hours at 60°C constant water bath with intermittent sonication. For THg determination, digestates were oxidized with bromine monochloride for at least 2 hours and pre-reduced with hydroxylamine hydrochloride. Subsamples were then reduced with stannous chloride, and the evolved Hg0 concentrations were determined by dual gold amalgamation cold vapor atomic fluorescence spectrometry (CVAFS) with a Tekran 2600 against both gaseous Hg0 and aqueous Hg2+ standards. MMHg determination used a direct ethylation method. Digestates were treated with ascorbic acid, buffered with acetate, and neutralized with potassium hydroxide (KOH; 45%) to pH between 4 and 5. Sodium tetraethylborate (1%, in 2% KOH) was added in digestates reacting for 20 minutes prior to analysis by CVAFS. The method detection limits for THg and MMHg were 26.5 femtomolar (fM) and 3.3 fM in the SSF, and 0.75 fM and 0.24 fM in the LSF, respectively.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For carbon (C) mass determination in the SSF and LSF, filters were packed into a tin capsule and analyzed for particulate carbon mass and measured using Carlo Erba 1108 elemental analyzer coupled to a Thermo Finningan Delta Plus XP IRMS at University of California, Santa Cruz. The analytical precision of C % is 0.65% in the SSF and 1.1% in the LSF based on the organic standards IU Acetanilide and USGC41.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/823406.rdf" xlink:title="OCE-1637632" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1637632 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1637632</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/908165.rdf" xlink:title="OCE-2023046" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2023046 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2023046</gmx:Anchor>
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The California Current Ecosystem (CCE) LTER data are managed by and available directly from the CCE project data site URL shown above. If there are any datasets listed below, they are data sets that were collected at or near the CCE LTER sampling locations, and funded by NSF OCE as ancillary projects related to the CCE LTER core research themes.&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;NSF Award Abstract (OCE-2224726)&lt;strong&gt;:&lt;/strong&gt;&lt;/strong&gt;&lt;br /&gt;
Coastal upwelling regions are found along the eastern boundaries of all ocean basins and are some of the most productive ecosystems in the ocean. This award is supporting the California Current Ecosystem Long Term Ecological Research (CCE LTER) site in a major upwelling biome. It leverages the 73-year California Cooperative Oceanic Fisheries Investigations (CalCOFI) program which provides essential information characterizing climate variability and change in this system. The CCE LTER addresses two over-arching questions: What are the mechanisms leading to ecological transitions in a coastal pelagic ecosystem? And what is the interplay between changing ocean climate, community structure, and ecosystem dynamics? The investigators are working towards diagnosing mechanisms of ecosystem change and developing a quantitative framework for forecasting future conditions and how these might affect the management of key living marine resources, including numerous fishes, invertebrates, marine mammals, and seabirds. They are training graduate and undergraduate students, as well as providing educational opportunities for teachers. Public programs and outreach efforts in collaboration with the Birch Aquarium at Scripps Institution of Oceanography are increasing public awareness and understanding of climate effects on coastal pelagic communities and connecting the public to cutting-edge ocean research.&lt;/p&gt;
&lt;p&gt;This project is adding to understanding of the mechanisms underlying abrupt ecological transitions with three interrelated foci: (1) investigation of marine heatwaves and resultant multiple stressors on organisms and communities, (2) elucidation of ecological stoichiometry and the response of multiple trophic levels to altered elemental ratios of source nutrients, and (3) analysis of top-down pressures mediated by a diverse suite of organisms. It is sustaining multi-scale measurements of five core LTER variables and responses to ocean warming, increased stratification, acidification, deoxygenation, and altered nutrient stoichiometry in the Northeast Pacific. The investigators are using long-term, spatially-resolved time series at multiple spatial scales to evaluate community shifts at multiple temporal scales, with new measurements allowing interrogation at finer taxonomic levels. They are conducting in situ multi-factorial experiments (temperature, macronutrients, micronutrients, light, grazing) in combination with genomic and transcriptomic analyses. These will complement time series measurements, inform next-generation biogeochemical models, and test hypotheses related to ecological stoichiometry and marine heatwaves. The team is also using a suite of imaging techniques, molecular and morphological methods, and active and passive acoustic approaches to quantify vertical structure and cooccurrence of organisms across trophic levels and test hypotheses about top-down control of the ecosystem.&lt;/p&gt;
&lt;p&gt;This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;This project is supported by continuing grants with name variations:&lt;/strong&gt;&lt;/p&gt;
&lt;ul&gt;
&lt;li&gt;LTER: Nonlinear transitions in the California Current Coastal Pelagic Ecosystem&lt;/li&gt;
&lt;li&gt;Ecological Transitions in the California Current Ecosystem: CCE-LTER Phase II&lt;/li&gt;
&lt;li&gt;LTER: CCE-LTER Phase III: Ecological Transitions in an Eastern Boundary Current Upwelling Ecosystem&lt;/li&gt;
&lt;li&gt;LTER: Ecosystem controls and multiple stressors in a coastal upwelling system - CCE IV&lt;/li&gt;
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This project will study how dimethylmercury is formed and removed in the oceans. Dimethylmercury is a naturally occurring compound. It is thought to be formed when man-made mercury is converted into monomethylmercury, a toxin that accumulates in fish. Despite representing a large fraction of mercury in the oceans, the origin and fate of dimethylmercury is not known. This research will use state-of-the-art analytical, genomic and modeling tools to address this information gap. It will also train graduate and undergraduate students to use field, experimental, and modeling methods. The results will be used in predictive models to forecast future trends in the oceanic mercury cycle. These models are needed to evaluate the effectiveness of international actions that seek to reverse increasing trends in the bioaccumulation of monomethylmercury in fish.&lt;/p&gt;
&lt;p&gt;Methylated mercury species in the ocean are formed in sediment and the water column from inorganic divalent mercury delivered from the atmosphere and rivers. The putative mechanism is a two-step process during which monomethylmercury is formed first, followed by slow methylation into dimethylmercury. The first step, biomethylation of divalent mercury into monomethylmercury, is relatively well-studied in sediment and known to be driven by sulfate- and iron-reducing bacteria and methanogens. The mechanism for monomethylmercury formation in the water column is less well understood, and the process of dimethylmercury formation in sediment or seawater is essentially unknown. Until recently, it was assumed that dimethylmercury represented a small enough fraction of the methylated and total mercury (the sum of all mercury species) pools to be insignificant in the global mercury cycle. Recent measurements, however, show that dimethylmercury levels in seawater can be in the same range as the other mercury species. This project will identify the biological and chemical methylating agents involved in the formation of dimethylmercury. Further, it will test the impact of varying biogeochemical conditions on dimethylmercury production. Results will be used to update the mercury module of the MIT General Circulation Model (MITgcm, a global biogeochemical model, and analyze the impacts of dimethylmercury production and degradation on monomethylmercury concentrations.&lt;/p&gt;
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&amp;lt;p&amp;gt;Total mercury (THg) and monomethylmercury (MMHg) in suspended and sinking particles were determined by digesting filter portions in nitric acid (2N, trace mental grade, Fisher) for 4 hours at 60°C constant water bath with intermittent sonication. For THg determination, digestates were oxidized with bromine monochloride for at least 2 hours and pre-reduced with hydroxylamine hydrochloride. Subsamples were then reduced with stannous chloride, and the evolved Hg0 concentrations were determined by dual gold amalgamation cold vapor atomic fluorescence spectrometry (CVAFS) with a Tekran 2600 against both gaseous Hg0 and aqueous Hg2+ standards. MMHg determination used a direct ethylation method. Digestates were treated with ascorbic acid, buffered with acetate, and neutralized with potassium hydroxide (KOH; 45%) to pH between 4 and 5. Sodium tetraethylborate (1%, in 2% KOH) was added in digestates reacting for 20 minutes prior to analysis by CVAFS. The method detection limits for THg and MMHg were 26.5 femtomolar (fM) and 3.3 fM in the SSF, and 0.75 fM and 0.24 fM in the LSF, respectively.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For carbon (C) mass determination in the SSF and LSF, filters were packed into a tin capsule and analyzed for particulate carbon mass and measured using Carlo Erba 1108 elemental analyzer coupled to a Thermo Finningan Delta Plus XP IRMS at University of California, Santa Cruz. The analytical precision of C % is 0.65% in the SSF and 1.1% in the LSF based on the organic standards IU Acetanilide and USGC41.&amp;lt;/p&amp;gt;</gco:CharacterString>
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&amp;quot;1&amp;quot; represents good value;&amp;lt;br /&amp;gt;
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Ambient water is drawn through a modular filter holder onto a 142-millimeter (mm) membrane without passing through the pump. The standard two-tier filter holder provides prefiltering and size fractioning. Collection targets include chlorophyll maximum, particulate trace metals, and phytoplankton. It features different flow rates and filter porosity to support a range of specimen collection. Sampling can be programmed to start at a scheduled time or begin with a countdown delay. It also features a dynamic pump speed algorithm that adjusts flow to protect the sample as material accumulates on the filter. Several pump options range from 0.5 to 30 liters per minute, with a max volume of 2,500 to 36,000 liters depending on the pump and battery pack used. The standard model is depth rated to 5,500 meters, with a deeper 7,000-meter option available. The operating temperature is -4 to 35 degrees Celsius.

The WTS-LV is available in four different configurations: Standard, Upright, Bore Hole, and Dual Filter Sampler. The high-capacity upright WTS-LV model provides three times the battery life of the standard model. The Bore-Hole WTS-LV is designed to fit through a narrow opening such as a 30-centimeter borehole. The dual filter WTS-LV features two vertical intake 142 mm filter holders to allow simultaneous filtering using two different porosities.</gco:CharacterString>
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