http://lod.bco-dmo.org/id/dataset/927918 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2024-05-20 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Temperature and light intensity measured at each transplant site during a reciprocal transplant experiment conducted at three sites in Turneffe Atoll, Belize from February to July 2022 2024-05-28 publication 2024-05-28 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2024-05-28 publication https://doi.org/10.26008/1912/bco-dmo.927918.1 Karina Scavo Lord Boston University principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Scavo Lord, K. (2024) Temperature and light intensity measured at each transplant site during a reciprocal transplant experiment conducted at three sites in Turneffe Atoll, Belize from February to July 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-05-28 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.927918.1 [access date] Coral transplant site environmental data Dataset Description: Methods and Sampling: &lt;p&gt;&lt;strong&gt;Reciprocal transplant experiment:&lt;/strong&gt;&lt;br /&gt; In February 2022, 32 colonies (subclone/ramet) of the Caribbean thin finger coral, &lt;em&gt;Porites divaricata&lt;/em&gt; (Taxonomy ID: 262287) were identified. 3 to 9 branch fragments were collected from every subclone (colony/ramet). 1 to 3 fragments from each ramet were moved to three designated sites: M1 (mangrove site 1), M2 (mangrove site 2), and R (reef site). 7 to 9 adjacent ropes were deployed at each site (seven ropes at M1 and R, and nine ropes at M2). Following a six-month period (February 2022 - July 2022), two traits were measured for each fragment: length change and red channel intensity, which serves as an inverse indicator of chlorophyll pigment density (Winters, Holzman, Blekhman, Beer, &amp;amp; Loya, 2009).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Environmental Measurements:&lt;/strong&gt;&lt;br /&gt; Four Onset HOBO Pendant data loggers (Onset Corporation, Bourne, MA) were deployed at the time of transplant to record both temperature (°C) and light intensity (lux) every two hours and remained there over the duration of the experiment. Lux was converted to photosynthetic photon flux density (PPFD: micromoles per square meter per second (µmol m-2 s-1)) with a standard sunlight conversion factor of 0.018.&lt;/p&gt; &lt;p&gt;See 'Related Datasets' for the coral trait variation data.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2126612 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2126612 completed Karina Scavo Lord Boston University 631-245-3393 2 Cummington Street Boston MA 02215 USA kscavo@bu.edu pointOfContact asNeeded Dataset Version: 1 Unknown Logger Site ISO_DateTime_Local ISO_DateTime_UTC Temp Light Site_Lat Site_Lon Onset HOBO (UA-002-64) Data Logger theme None, User defined site ISO_DateTime_Local ISO_DateTime_UTC water temperature at measurement depth light intensity latitude longitude featureType BCO-DMO Standard Parameters Onset HOBO Pendant Temperature/Light Data Logger instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. OCE-PRF: Drivers of phenotypic diversity and adaptation in asexually propagating coral populations https://osprey.bco-dmo.org/project/880237 OCE-PRF: Drivers of phenotypic diversity and adaptation in asexually propagating coral populations <p><em>NSF Award Abstract:</em><br /> This award is funded in whole or in part under the American Rescue Plan Act of 2021 (Public Law 117-2).</p> <p>In this era of rapid environmental change and degradation, the survival of marine organisms will depend largely upon their ability to tolerate increasing environmental stress. However, the processes that drive resilience (e.g., the relative contribution of genetic versus environmental factors) remain largely a mystery. Corals are a major focus of current studies on organismal resilience because they are undergoing a worldwide decline, and they are so important for both the health of the oceans and the welfare of coastal communities. Corals often occupy and build habitats through asexual reproduction (production of clones), and it is widely assumed that such asexual populations may lack sufficient genetic diversity to respond to diverse environmental stressors. However, few previous studies have quantified the effects of asexual reproduction on the ability of corals to respond to environmental change. This research explores the different mechanisms that contribute to differences in coral stress response among two predominately clonal populations of the Caribbean thin finger coral (<em>Porites divaricata</em>) dwelling in distinct habitat types (mangroves vs. reef). This research will illuminate the drivers of organismal resilience, potentially impacting ongoing coral conservation and restoration efforts. It will also broaden the participation of underrepresented groups by encouraging active participation by local community members, students and scientists at the University of Belize, as well as providing unique training opportunities for next-generation scientists at the interface of field marine ecology and genomics. Importantly, these results will be communicated to a wide audience through diverse venues, including technical reports and management recommendations provided for government agencies and non-profit Belizean conservation organizations, popular articles and curricular materials for the community at large, and peer-reviewed manuscripts and presentations targeted to the scientific community.</p> <p>Among conservation biologists and ecologists, there is an urgent effort underway to understand the causes of diversity in traits impacting organismal survival and reproduction. If we are to understand how natural populations will respond to environmental change, it is critical to understand how genetic, epigenetic, and environmental factors impact resilience. Such studies have advanced significantly in many plant species, but they are only just beginning to be applied to animals like corals. As in many plants, the model species used here - Caribbean thin finger coral (<em>Porites divaricata</em>) - reproduces primarily asexually, allowing us to compare the performance of clones in different environments and isolate the effects of genotype, epigenetics (namely DNA methylation), environment, and somatic mutations on variation in stress-related coral traits. Specially, combining fully-crossed reciprocally transplanted coral ramets across mangrove and reef sites with genomic and methylation sequencing data, this research will evaluate 1) the role of intra-genet variation accumulated during asexual reproduction in facilitating adaptation in the mangrove population, relative to roles of between-genet variation and phenotypic plasticity, 2) assess the role of new DNA methylation states, and 3) measure the rate of accumulation of new DNA methylation marks and base-changes during asexual reproduction. Results from this research effort will advance our understanding of the role of mechanisms like DNA methylation and somatic mutations in driving phenotypic variation in critical stress-response traits and how these mutations accumulate over time, provide insight into how such mechanisms are generated and inherited across asexual generations, build upon the sparse understanding of the role of novel habitat types, i.e. mangroves, in coral ecology and evolution; and generate novel molecular resources including the first reference genome assembly for the non-model coral, <em>P. divaricata</em>.</p> <p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> Adaptation in asexual coral populations largerWorkCitation project eng; USA oceans -87.815167 -87.81111 17.28717 17.288681 2022-02-11 2022-07-20 Caribbean 0 BCO-DMO catalogue of parameters from Temperature and light intensity measured at each transplant site during a reciprocal transplant experiment conducted at three sites in Turneffe Atoll, Belize from February to July 2022 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/927938.rdf Name: Logger Units: unitless Description: <p>Description of site location where logger was placed: channel, island, or reef (two loggers were placed at the reef - reef_1 and reef_2)</p> http://lod.bco-dmo.org/id/dataset-parameter/927939.rdf Name: Site Units: unitless Description: <p>Site where logger was placed: M2 = mangrove site 2, M1= mangrove site 1, and R = reef (two loggers were placed at R- R_1 and R_2)</p> http://lod.bco-dmo.org/id/dataset-parameter/927940.rdf Name: ISO_DateTime_Local Units: unitless Description: <p>Date and time when the data were collected in ISO 8601 format; local time zone = CDT/CST</p> http://lod.bco-dmo.org/id/dataset-parameter/927941.rdf Name: ISO_DateTime_UTC Units: unitless Description: <p>Date and time when the data were collected in ISO 8601 format; UTC time zone</p> http://lod.bco-dmo.org/id/dataset-parameter/927942.rdf Name: Temp Units: degrees Celsius Description: <p>Temperature of seawater at logging point</p> http://lod.bco-dmo.org/id/dataset-parameter/927943.rdf Name: Light Units: photosynthetic photon flux density (PPFD) Description: <p>Light intensity at depth at logging point</p> http://lod.bco-dmo.org/id/dataset-parameter/928940.rdf Name: Site_Lat Units: decimal degrees Description: <p>General latitude of the site (not necessarily the exact latitude where the logger was placed within the site); positive values = North</p> http://lod.bco-dmo.org/id/dataset-parameter/928942.rdf Name: Site_Lon Units: decimal degrees Description: <p>General longitude of the site (not necessarily the exact longitude where the logger was placed within the site); negative values = West</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 621768 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/99df26a4-c651-4185-89d1-917bacb1880c/content download https://doi.org/10.26008/1912/bco-dmo.927918.1 download onLine dataset &lt;p&gt;&lt;strong&gt;Reciprocal transplant experiment:&lt;/strong&gt;&lt;br /&gt; In February 2022, 32 colonies (subclone/ramet) of the Caribbean thin finger coral, &lt;em&gt;Porites divaricata&lt;/em&gt; (Taxonomy ID: 262287) were identified. 3 to 9 branch fragments were collected from every subclone (colony/ramet). 1 to 3 fragments from each ramet were moved to three designated sites: M1 (mangrove site 1), M2 (mangrove site 2), and R (reef site). 7 to 9 adjacent ropes were deployed at each site (seven ropes at M1 and R, and nine ropes at M2). Following a six-month period (February 2022 - July 2022), two traits were measured for each fragment: length change and red channel intensity, which serves as an inverse indicator of chlorophyll pigment density (Winters, Holzman, Blekhman, Beer, &amp;amp; Loya, 2009).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Environmental Measurements:&lt;/strong&gt;&lt;br /&gt; Four Onset HOBO Pendant data loggers (Onset Corporation, Bourne, MA) were deployed at the time of transplant to record both temperature (°C) and light intensity (lux) every two hours and remained there over the duration of the experiment. Lux was converted to photosynthetic photon flux density (PPFD: micromoles per square meter per second (µmol m-2 s-1)) with a standard sunlight conversion factor of 0.018.&lt;/p&gt; &lt;p&gt;See 'Related Datasets' for the coral trait variation data.&lt;/p&gt; Specified by the Principal Investigator(s) - Imported original file &quot;EnvironmentalSite_Data.xlsx&quot; into the BCO-DMO system. - Converted the date and time columns to ISO 8601 format in both (CST/CDT) and UTC time zones. - Added columns for the site latitude and longitude. - Removed the original date and time columns. - Saved the final file as &quot;927918_v1_coral_transplant_site_environmental_data.csv&quot;. Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Onset HOBO (UA-002-64) Data Logger Onset HOBO (UA-002-64) Data Logger PI Supplied Instrument Name: Onset HOBO (UA-002-64) Data Logger PI Supplied Instrument Description:The environmental data (temperature and light intensity) was measured by the following instrument: Onset HOBO (UA-002-64) Data Logger Measuring Temperature and Light Intensity. Instrument Name: Onset HOBO Pendant Temperature/Light Data Logger Instrument Short Name:Onset HOBO Pendant Temp/Light Instrument Description: The Onset HOBO (model numbers UA-002-64 or UA-001-64) is an in-situ instrument for wet or underwater applications. It supports light intensity, soil temperature, temperature, and water temperature. A two-channel logger with 10-bit resolution can record up to approximately 28,000 combined temperature and light measurements with 64K bytes memory. It has a polypropylene housing case. Uses an optical USB to transmit data. A solar radiation shield is used for measurement in sunlight. Temperature measurement range: -20 deg C to 70 deg C (temperature). Light measurement range: 0 to 320,000 lux. Temperature accuracy: +/- 0.53 deg C from 0 deg C to 50 deg C. Light accuracy: Designed for measurement of relative light levels. Water depth rating: 30 m.