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            <gco:CharacterString>Cite this dataset as: Mulholland, M., Chappell, P. D., Selden, C. (2024) Hydrography, nutrients, and nitrogen uptake rates from SPIROPA cruise TN368 in July 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-05-20 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/927927 [access date]</gco:CharacterString>
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        <gco:CharacterString>Hydrography, nutrients, and uptake rates (SPIROPA, July 2019) Dataset Description: &amp;lt;p&amp;gt;This dataset is derived from Selden et al. (2024) JGR Oceans Table S1, and McGillicuddy bottle data from TN368, the third SPIROPA cruise (https://www.bco-dmo.org/dataset/849340). The mean values of nitrogen fixation rates shown here are derived from nitrogen fixation incubation data (https://www.bco-dmo.org/dataset/928568)&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Glossary of terms:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
A = Atom-% enrichment&amp;lt;br /&amp;gt;
APN = Atom-% enrichment of the particulate nitrogen pool&amp;amp;nbsp;&amp;lt;br /&amp;gt;
BDL = Below detection limit&amp;lt;br /&amp;gt;
DNQ = Detectable but not quantifiable&amp;lt;br /&amp;gt;
LOD = Limit of detection&amp;lt;br /&amp;gt;
LOQ = Limit of quantification&amp;amp;nbsp;&amp;lt;br /&amp;gt;
N2 = Nitrogen gas&amp;lt;br /&amp;gt;
PETG = Polyethylene Terephthalate Glycol&amp;lt;br /&amp;gt;
PN = Particulate nitrogen&amp;lt;br /&amp;gt;
NFR = Nitrogen fixation rate&amp;lt;br /&amp;gt;
SUR = Specific uptake rate&amp;lt;br /&amp;gt;
t0 = time initial&amp;lt;br /&amp;gt;
tf = time final&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;div class=&amp;quot;field&amp;quot; id=&amp;quot;form_location&amp;quot;&amp;gt;
&amp;lt;p&amp;gt;Nitrogen fixation rates and hydrographic data were measured in July 2019 in&amp;amp;nbsp;conjunction with the SPIROPA (Shelfbreak Productivity Interdisciplinary Research Operation at the Pioneer Array)&amp;amp;nbsp;project cruises (Chief Scientist Dr. Dennis McGillicuddy).&amp;amp;nbsp;This case study was conducted at the Mid-Atlantic Bight shelfbreak front to the south of Massachusetts, USA to examine whether significant N2 fixation occurs in the continental shelf region. On July 5th, 2019, the&amp;amp;nbsp;vessel R/V Thomas G. Thompson (cruise TN368) departed Woods Hole, MA and performed multiple transects in the&amp;amp;nbsp;area of 70 to 71 degrees West longitude and 39 to 40.5 degrees North latitude. Sampling was restricted to waters above the 0.1% light level.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Water was collected in 10L Niskin bottles on a 24-bottle rosette.&amp;amp;nbsp;Vertical conductivity, temperature, and depth profiles were measured&amp;amp;nbsp;concurrently using a Sea-Bird 911+ CTD profiler. Samples to determine nitrate, phosphate, and silicate concentrations were filtered (0.4 um, polycarbonate) directly from the Niskin bottles into acid-washed polyethylene bottles and stored&amp;amp;nbsp;frozen until analysis.&amp;amp;nbsp; Standard&amp;amp;nbsp;colorimetric methods were performed at the Woods Hole Oceanographic Institution's Nutrient Analytical Facility using a SEAL Analytical AA3 HR discrete chemistry analyzer. Detection limits for nitrate, phosphate, and silicate methods were 0.040, 0.030 and 0.009 μM, respectively.&amp;lt;/p&amp;gt;

&amp;lt;div&amp;gt;This dataset presents hydrographic and nutrient data plus uptake rates and nitrogen fixation rates based on the mean values of replicate samples at each collection site and depth. For full incubation data (and methods)&amp;amp;nbsp;for all replicates, please refer to BCO-DMO dataset&amp;amp;nbsp;https://www.bco-dmo.org/dataset/928568.&amp;lt;/div&amp;gt;

&amp;lt;p&amp;gt;Nitrogen fixation rates (NFR) were measured&amp;amp;nbsp;using the&amp;amp;nbsp;&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;&amp;amp;nbsp;bubble release method&amp;amp;nbsp;on repeat cross-frontal transects. Bottles were incubated on-deck for approximately 24 hours in incubators that recirculated water through aquaria chillers to maintain approximate temperature conditions for shelf, frontal, and offshore waters. At the end of the incubation period, an aliquot was collected from each bottle for isotope analysis (see&amp;amp;nbsp;https://www.bco-dmo.org/dataset/928568).&amp;amp;nbsp;The remaining incubation volume was then filtered for initial particulate nitrogen (PN) samples.&amp;amp;nbsp;&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Specific uptake rates were calculated following Montoya et al. (1996), with equations detailed below.&amp;lt;/p&amp;gt;
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&lt;p&gt;The continental shelf break of the Middle Atlantic Bight supports a productive and diverse ecosystem. Current paradigms suggest that this productivity is driven by several upwelling mechanisms at the shelf break front. This upwelling supplies nutrients that stimulate primary production by phytoplankton, which in turn leads to enhanced production at higher trophic levels. Although local enhancement of phytoplankton biomass has been observed in some circumstances, such a feature is curiously absent from time-averaged measurements, both from satellites and shipboard sampling. Why would there not be a mean enhancement in phytoplankton biomass as a result of the upwelling? One hypothesis is that grazing by zooplankton prevents accumulation of biomass on seasonal and longer time scales, transferring the excess production to higher trophic levels and thereby contributing to the overall productivity of the ecosystem. However, another possibility is that the net impact of these highly intermittent processes is not adequately represented in long-term means of the observations, because of the relatively low resolution of the in-water measurements and the fact that the frontal enhancement can take place below the depth observable by satellite. The deployment of the Ocean Observatories Initiative (OOI) Pioneer Array south of New England has provided a unique opportunity to test these hypotheses. The combination of moored instrumentation and autonomous underwater vehicles will facilitate observations of the frontal system with unprecedented spatial and temporal resolution. This will provide an ideal four-dimensional (space-time) context in which to conduct a detailed study of frontal dynamics and plankton communities needed to examine mechanisms controlling phytoplankton populations in this frontal system. This project will also: (1) promote teaching, training and learning via participation of graduate and undergraduate students in the research , (2) provide a broad dissemination of information by means of outreach in public forums, printed media, and a video documentary of the field work, and (3) contribute to improving societal well-being and increased economic competitiveness by providing the knowledge needed for science-based stewardship of coastal ecosystems, with particular emphasis on connecting with the fishing industry through the Commercial Fisheries Research Foundation.&lt;/p&gt;
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http://lod.bco-dmo.org/id/dataset-parameter/929419.rdf
	Name: Latitude
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http://lod.bco-dmo.org/id/dataset-parameter/929420.rdf
	Name: Longitude
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http://lod.bco-dmo.org/id/dataset-parameter/929421.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/929422.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/929423.rdf
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	Units: meters (m)
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http://lod.bco-dmo.org/id/dataset-parameter/929424.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/929425.rdf
	Name: Temperature
	Units: degrees Celsius (°C)
	Description: &lt;p&gt;Temperature in degrees Celsius (derived from CTD data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/929426.rdf
	Name: SigmaTheta
	Units: kilograms per cubic meter (kg/m3)
	Description: &lt;p&gt;Sigma theta potential density at sampling depth (derived from CTD data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/929427.rdf
	Name: NO3
	Units: micromolar
	Description: &lt;p&gt;Nitrate concentration of sample water&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/929428.rdf
	Name: PO4
	Units: micromolar
	Description: &lt;p&gt;Phosphate concentration of sample water&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/929429.rdf
	Name: SiO4
	Units: micromolar
	Description: &lt;p&gt;Silicate concentration of sample water&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/929430.rdf
	Name: PN_mean
	Units: micromolar
	Description: &lt;p&gt;Mean particulate nitrate concentration of sample water&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/929431.rdf
	Name: PN_sd
	Units: micromolar
	Description: &lt;p&gt;Standard deviation of particulate nitrate concentrations of sample water&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/929432.rdf
	Name: Replicate_incubations
	Units: unitless
	Description: &lt;p&gt;Number of replicate 15N2 incubations conducted&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/929433.rdf
	Name: Repl_spl_ids
	Units: unitless
	Description: &lt;p&gt;Sample ID of replicate samples in incubation&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/929434.rdf
	Name: Flag
	Units: unitless
	Description: &lt;p&gt;Flag for specific uptake rate/nitrogen (N2) fixation rate, where DNQ = Detected but not quantifiable; BDL = Below detection limit&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/929435.rdf
	Name: SUR_mean
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	Description: &lt;p&gt;Mean specific N2 uptake rate calculated from replicate incubations&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/929436.rdf
	Name: SUR_sd
	Units: per day
	Description: &lt;p&gt;Standard deviation of specific nitrogen uptake rates&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/929437.rdf
	Name: NFR_mean
	Units: nanomoles nitrogen per liter per day (nmol N2/L/day)
	Description: &lt;p&gt;Mean nitrogen fixation rate calculated from replicate incubations&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/929438.rdf
	Name: NFR_sd
	Units: nanomoles nitrogen per liter per day (nmol N2/L/day)
	Description: &lt;p&gt;Standard deviation of nitrogen fixation rates&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/929439.rdf
	Name: NFR_LOD
	Units: nanomoles nitrogen per liter per day (nmol N2/L/day)
	Description: &lt;p&gt;Limit of detection for nitrogen fixation rate at given sampling location&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/929440.rdf
	Name: NFR_LOQ
	Units: nanomoles nitrogen per liter per day (nmol N2/L/day)
	Description: &lt;p&gt;Limit of quantification for nitrogen fixation rate at given sampling location&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/929441.rdf
	Name: SUR_LOD
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	Description: &lt;p&gt;Limit of detection for specific nitrogen uptake rate at given sampling location&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/929442.rdf
	Name: SUR_LOQ
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http://lod.bco-dmo.org/id/dataset-parameter/929443.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/929444.rdf
	Name: ISO_DateTime_UTC_if_EDT
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&amp;lt;p&amp;gt;Nitrogen fixation rates and hydrographic data were measured in July 2019 in&amp;amp;nbsp;conjunction with the SPIROPA (Shelfbreak Productivity Interdisciplinary Research Operation at the Pioneer Array)&amp;amp;nbsp;project cruises (Chief Scientist Dr. Dennis McGillicuddy).&amp;amp;nbsp;This case study was conducted at the Mid-Atlantic Bight shelfbreak front to the south of Massachusetts, USA to examine whether significant N2 fixation occurs in the continental shelf region. On July 5th, 2019, the&amp;amp;nbsp;vessel R/V Thomas G. Thompson (cruise TN368) departed Woods Hole, MA and performed multiple transects in the&amp;amp;nbsp;area of 70 to 71 degrees West longitude and 39 to 40.5 degrees North latitude. Sampling was restricted to waters above the 0.1% light level.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Water was collected in 10L Niskin bottles on a 24-bottle rosette.&amp;amp;nbsp;Vertical conductivity, temperature, and depth profiles were measured&amp;amp;nbsp;concurrently using a Sea-Bird 911+ CTD profiler. Samples to determine nitrate, phosphate, and silicate concentrations were filtered (0.4 um, polycarbonate) directly from the Niskin bottles into acid-washed polyethylene bottles and stored&amp;amp;nbsp;frozen until analysis.&amp;amp;nbsp; Standard&amp;amp;nbsp;colorimetric methods were performed at the Woods Hole Oceanographic Institution's Nutrient Analytical Facility using a SEAL Analytical AA3 HR discrete chemistry analyzer. Detection limits for nitrate, phosphate, and silicate methods were 0.040, 0.030 and 0.009 μM, respectively.&amp;lt;/p&amp;gt;

&amp;lt;div&amp;gt;This dataset presents hydrographic and nutrient data plus uptake rates and nitrogen fixation rates based on the mean values of replicate samples at each collection site and depth. For full incubation data (and methods)&amp;amp;nbsp;for all replicates, please refer to BCO-DMO dataset&amp;amp;nbsp;https://www.bco-dmo.org/dataset/928568.&amp;lt;/div&amp;gt;

&amp;lt;p&amp;gt;Nitrogen fixation rates (NFR) were measured&amp;amp;nbsp;using the&amp;amp;nbsp;&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;&amp;amp;nbsp;bubble release method&amp;amp;nbsp;on repeat cross-frontal transects. Bottles were incubated on-deck for approximately 24 hours in incubators that recirculated water through aquaria chillers to maintain approximate temperature conditions for shelf, frontal, and offshore waters. At the end of the incubation period, an aliquot was collected from each bottle for isotope analysis (see&amp;amp;nbsp;https://www.bco-dmo.org/dataset/928568).&amp;amp;nbsp;The remaining incubation volume was then filtered for initial particulate nitrogen (PN) samples.&amp;amp;nbsp;&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Specific uptake rates were calculated following Montoya et al. (1996), with equations detailed below.&amp;lt;/p&amp;gt;
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                <gco:CharacterString>&amp;lt;p&amp;gt;Specific N&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;&amp;amp;nbsp;uptake rates (SUR) were calculated following Montoya et al. (1996):&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Equation 1:&amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp;SUR = (APN_tf - APN_t0) / (AN2 - APN_t0) ] x (1/time)&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
where A represents the atom-% enrichment of the initial (t=0) or final (t=f) particulate nitrogen (PN)&amp;amp;nbsp;pool, or the nitrogen (N&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;) pool. Across all experiments, AN2 averaged 3.89 ± 2.0%. Experiments with &amp;amp;lt;1% enrichment were excluded from downstream analysis. The specific uptake rate represents the relative contribution of diazotroph-derived N to PN turnover within a given sample. Absolute N&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;&amp;amp;nbsp;fixation rates (NFR) were calculated as:&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Equation 2:&amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp;NFR = SUR x [PN]&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
where [PN] indicates the particulate nitrogen concentration. Limits of detection and quantification were calculated per incubation by propagating the minimum detectable difference between initial and final&amp;amp;nbsp;&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N atom-% enrichment of the PN pool (3σ and 10σ), three sigma and ten sigma respectively, n≥7, 12.5 µg N standards measured daily&amp;amp;nbsp;through Eqn. 2 (Ripp, 1996; White et al. 2020). A rate was only considered detectable if two of three replicate incubations yielded detectable rates.&amp;lt;/p&amp;gt;</gco:CharacterString>
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                  <gco:CharacterString>- Imported data from source file &amp;quot;Selden_JGR24_TableS1.xlsx&amp;quot; into the BCO-DMO data system. 
- Changed date format from m/d/yyyy to yyyy-mm-dd (ISO Date 8601 format) and converted datetimes from EST to UTC.
- Converted latitude and longitude to decimal degrees.
- Added official cruise ID corresponding to the the provided deployment and cruise name
- Added cast number and replicate sample ids (from the incubations) based on datetime and depth.  
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