http://lod.bco-dmo.org/id/dataset/928980
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-05-28
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Chlorphyll a and pheophytin from two cruises performed as part of the STING project from R/V Atlantic Explorer AE2305 (Sting I cruise) and R/V Endeavor EN704 (Sting II cruise) in the Gulf of Mexico near Florida from February to July 2023
2024-05-28
publication
2024-05-28
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-07-26
publication
https://doi.org/10.26008/1912/bco-dmo.928980.1
Phoebe Dreux Chappell
University of South Florida
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Chappell, P. D., Confesor, K. (2024) Chlorphyll a and pheophytin from two cruises performed as part of the STING project from R/V Atlantic Explorer AE2305 (Sting I cruise) and R/V Endeavor EN704 (Sting II cruise) in the Gulf of Mexico near Florida from February to July 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-05-28 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.928980.1 [access date]
Chlorphyll a and pheophytin from two cruises performed as part of the STING project Dataset Description: Methods and Sampling: <p>Whole water was collected using three methods: tow-fish, surface pump deployed on a metal-free line, or ctd rosette. There were three "types" of samples: surface (collected from one of the two pumps - towfish or surface stationary), chl max (collected at the chl maximum from one of the two CTDs, conventional or trace-metal clean), and profile.</p>
<p>Whole water (300 - 4300 ml) was filtered onto 25 mm GF/F filters (nominal pore size 0.7 um) using a GAST vacuum pump and and kept frozen (at -20 degrees C) in the dark until analysis. Filters were extracted overnight (16-18 hrs) in 90% acetone before analysis on a Trilogy Fluorometer with the chl a extracted acidification module calibrated using Turner Chl a standards following the extraction protocol of Strickland and Parsons 1972 (Strickland, J. D. H., &amp; Parsons, T. R. (1972). <em>A Practical Handbook of Seawater Analysis</em>,<em> </em>2nd edition. Fisheries Research Board of Canada.). Following the initial fluorescence reading, 20 ul of 10% HCl was added and another fluorescence reading was recorded.</p>
<p>The surface pump on STING I was a Wilden Pro-Flo Series P100 PTPE pump. The surface pump on STING II and for all tow-fish samples was an Almatec E15TTT pump. All pump samples were pumped through Teflon tubing. The CTD samples were collected via 10 L Niskin bottles. The fluorometer was a Turner Designs Trilogy Fluorometer with the chl a acidification method module. Calibration was done using chl a standards from Turner.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2341997 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2341997
completed
Phoebe Dreux Chappell
University of South Florida
USF St. Petersburg campus 140 7th Ave. South
St. Petersburg
FL
33701
USA
dreux@usf.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cruise
Station
Lat
Lon
Depth
Date
STING_ID
Collection
Type
Chla
Pheophytin
Niskin bottles
surface pump
tow-fish
Turner Designs Trilogy Fluorometer
theme
None, User defined
Cruise identfier
station id
latitude
longitude
depth
date
sample identification
sampling_method
sample type
chlorophyll a
total phaeopigment
featureType
BCO-DMO Standard Parameters
Niskin bottle
Pump
towed unmanned submersible
Turner Designs Trilogy fluorometer
instrument
BCO-DMO Standard Instruments
AE2305
EN704
service
Deployment Activity
Gulf of Mexico near Florida
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Linking iron and nitrogen sources in an oligotrophic coastal margin: Nitrogen fixation and the role of boundary fluxes
https://osprey.bco-dmo.org/project/880516
Collaborative Research: Linking iron and nitrogen sources in an oligotrophic coastal margin: Nitrogen fixation and the role of boundary fluxes
<p><strong>NSF Award Abstract:</strong><br />
This project will investigate how groundwater discharge delivers important nutrients to the coastal ecosystems of the West Florida Shelf. Preliminary studies indicate that groundwater may supply both dissolved organic nitrogen (DON) and iron in this region. In coastal ecosystems like the West Florida Shelf that have very low nitrate and ammonium concentrations, DON is the main form of nitrogen available to organisms. Nitrogen cycling is strongly affected by iron availability because iron is essential for both photosynthesis and for nitrogen fixation. This study will investigate the sources and composition of DON and iron, and their influence on the coastal ecosystem. The team will sample offshore groundwater wells, river and estuarine waters, and conduct two expeditions across the West Florida Shelf in winter and summer. Investigators will participate in K-12 and outreach activities to increase awareness of the project and related science. The project will fund the work of six graduate and eight undergraduate students across five institutions, furthering NSF’s goals of education and training.</p>
<p>Motivated by preliminary observations of unexplained, tightly-correlated DON and dissolved iron concentrations across the West Florida Shelf (WFS), the proposed work will quantify the flux and isotopic signatures of submarine groundwater discharge (SGD)-derived DON and iron to the WFS, and evaluate the bioavailability of this temporally-variable source using four seasonal near-shore campaigns sampling offshore groundwater wells, estuarine, and riverine endmembers and two cross-shelf cruises. The work will evaluate whether SGD stimulates nitrogen fixation on the WFS, and the potential for the stimulated nitrogen fixation to further modify the chemistry of DON and dissolved iron in the region. The cross-shelf cruises will investigate hypothesized periods of maximum SGD and Trichodesmium abundance (June), and reduced river discharge and SGD (February), thus comparing two distinct biogeochemical regimes. The concentrations and isotopic compositions of DON and dissolved iron, molecular composition of DON, and the concentration and composition of iron-binding ligands will be characterized. Nitrogen fixation rates and Trichodesmium spp. abundance and expression of iron stress genes will be measured. Fluxes of DON and iron from SGD and rivers will be quantified with radium isotope mass balances. The impacts of SGD on nitrogen fixation and DON/ligand production will be constrained with incubations of natural phytoplankton communities with submarine groundwater amendments. Two hypotheses will be tested: 1) SGD is the dominant source of bioavailable DON and dissolved iron on the WFS, and 2) SGD-alleviation of iron stress changes the dominant Trichodesmium species on the WFS, increases nitrogen fixation rates and modifies DON and iron composition. Overall, the work will establish connections between marine nitrogen and iron cycling and evaluate the potential for coastal inputs to modify water along the WFS before export to the Atlantic Ocean. This study will thus provide a framework to consider these boundary fluxes in oligotrophic coastal systems and the relative importance of rivers and SGD as sources of nitrogen and iron in other analogous locations, such as coastal systems in Australia, India, and Africa, where nitrogen fixation and SGD have also been documented.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
West Florida Shelf DON and Fe
largerWorkCitation
project
eng; USA
oceans
Gulf of Mexico near Florida
-86.566932
-82.515283
26.13205
28.498467
2023-02-20
2023-07-12
Gulf of Mexico/America, West Florida Shelf
0
BCO-DMO catalogue of parameters from Chlorphyll a and pheophytin from two cruises performed as part of the STING project from R/V Atlantic Explorer AE2305 (Sting I cruise) and R/V Endeavor EN704 (Sting II cruise) in the Gulf of Mexico near Florida from February to July 2023
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/933273.rdf
Name: Cruise
Units: unitless
Description: <p>which cruise sample was collected: STINGI or STINGII</p>
http://lod.bco-dmo.org/id/dataset-parameter/933274.rdf
Name: Station
Units: unitless
Description: <p>Station identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/933275.rdf
Name: Lat
Units: decimal degrees
Description: <p>Sampling location latitude, south is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/933276.rdf
Name: Lon
Units: decimal degrees
Description: <p>Sampling location longitude, west is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/933277.rdf
Name: Depth
Units: meters (m)
Description: <p>Depth of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/933278.rdf
Name: Date
Units: unitless
Description: <p>Date of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/933279.rdf
Name: STING_ID
Units: unitless
Description: <p>Project specific identification number, which is unique to each sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/933280.rdf
Name: Collection
Units: unitless
Description: <p>Method of collection of water sample. fish = towfish, koubapump = stationary teflon pump, tmctd = trace metal ctd rosette, shipctd = conventional ctd rosette</p>
http://lod.bco-dmo.org/id/dataset-parameter/933281.rdf
Name: Type
Units: unitless
Description: <p>Type of sample collection: surface = (collected from one of the two pumps - towfish or surface stationary), chl max = (collected at the chl maximum from one of the two CTDs, conventional or trace-metal clean), profile = (water column samples not collected at the chl max and classified as a different profile type called "other depth").</p>
http://lod.bco-dmo.org/id/dataset-parameter/933282.rdf
Name: Chla
Units: micrograms/liter (ug/l)
Description: <p>Chlorophyll a concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/933283.rdf
Name: Pheophytin
Units: micrograms/liter (ug/l)
Description: <p>Pheophytin concentration</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
10000
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/233925d2-5117-4383-a058-e7f0e1e7dbb6/content
download
https://doi.org/10.26008/1912/bco-dmo.928980.1
download
onLine
dataset
<p>Whole water was collected using three methods: tow-fish, surface pump deployed on a metal-free line, or ctd rosette. There were three "types" of samples: surface (collected from one of the two pumps - towfish or surface stationary), chl max (collected at the chl maximum from one of the two CTDs, conventional or trace-metal clean), and profile.</p>
<p>Whole water (300 - 4300 ml) was filtered onto 25 mm GF/F filters (nominal pore size 0.7 um) using a GAST vacuum pump and and kept frozen (at -20 degrees C) in the dark until analysis. Filters were extracted overnight (16-18 hrs) in 90% acetone before analysis on a Trilogy Fluorometer with the chl a extracted acidification module calibrated using Turner Chl a standards following the extraction protocol of Strickland and Parsons 1972 (Strickland, J. D. H., &amp; Parsons, T. R. (1972). <em>A Practical Handbook of Seawater Analysis</em>,<em> </em>2nd edition. Fisheries Research Board of Canada.). Following the initial fluorescence reading, 20 ul of 10% HCl was added and another fluorescence reading was recorded.</p>
<p>The surface pump on STING I was a Wilden Pro-Flo Series P100 PTPE pump. The surface pump on STING II and for all tow-fish samples was an Almatec E15TTT pump. All pump samples were pumped through Teflon tubing. The CTD samples were collected via 10 L Niskin bottles. The fluorometer was a Turner Designs Trilogy Fluorometer with the chl a acidification method module. Calibration was done using chl a standards from Turner.</p>
Specified by the Principal Investigator(s)
<p>Duplicates from each sample were averaged together.</p>
Specified by the Principal Investigator(s)
1. Loaded submitted file STING_Chl_ToSubmit.csv into BCO-DMO data processing system laminar.
2. Converted date from the format %d-%b-%Y to an ISO date format of %Y-%m-%d
3. Renamed parameters with units in their name by removing the units from the text. For example, renamed 'Pheophytin_ugperL' to 'Pheophytin'
4. Reordered parameter columns so that the metadata parameters location and date come before data results.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Niskin bottles
Niskin bottles
PI Supplied Instrument Name: Niskin bottles PI Supplied Instrument Description:10 L Niskin bottles Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
surface pump
surface pump
PI Supplied Instrument Name: surface pump PI Supplied Instrument Description:The surface pump on STING I was a Wilden Pro-Flo Series P100 PTPE pump. The surface pump on STING II and for all tow-fish samples was an Almatec E15TTT pump. All pump samples were pumped through Teflon tubing. Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps
tow-fish
tow-fish
PI Supplied Instrument Name: tow-fish Instrument Name: towed unmanned submersible Instrument Short Name:towed unmanned submersible Instrument Description: A vehicle towed by rigid cable through the water column at fixed or varying depth with no propulsion and no human operator (e.g. Towfish, Scanfish, UOR, SeaSoar).
Turner Designs Trilogy Fluorometer
Turner Designs Trilogy Fluorometer
PI Supplied Instrument Name: Turner Designs Trilogy Fluorometer PI Supplied Instrument Description:Turner Designs Trilogy Fluorometer with the chl a acidification method module. Calibration was done using chl a standards from Turner. Instrument Name: Turner Designs Trilogy fluorometer Instrument Short Name:Turner Trilogy Instrument Description: The Trilogy Laboratory Fluorometer is a compact laboratory instrument for making fluorescence, absorbance, and turbidity measurements using the appropriate snap-in application module. Fluorescence modules are available for discrete sample measurements of various fluorescent materials including chlorophyll (in vivo and extracted), rhodamine, fluorescein, cyanobacteria pigments, ammonium, CDOM, optical brighteners, and other fluorescent compounds.
Cruise: AE2305
AE2305
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
AE2305
Angela N. Knapp
Florida State University
Cruise: EN704
EN704
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN704
Angela N. Knapp
Florida State University
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel