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        <gco:CharacterString>CliOMZ AT50-10 AUV Clio Data: Clio Dive Log Dataset Description:  Methods and Sampling: &amp;lt;p&amp;gt;Dive log for Clio dives 031-050. In depth operation summary for each dive can be found in the Clio Operations Report (see related publications).&amp;lt;/p&amp;gt;</gco:CharacterString>
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Though scarce and largely insoluble, trace metals are key components of sophisticated enzymes (protein molecules that speed up biochemical reactions) involved in biogeochemical cycles in the dark ocean (below 1000m). For example, metalloenzymes are involved in nearly every reaction in the nitrogen cycle. Yet, despite direct connections between trace metal and nitrogen cycles, the relationship between trace metal distributions and biological nitrogen cycling processes in the dark ocean have rarely been explored, likely due to the technical challenges associated with their study. Availability of the autonomous underwater vehicle (AUV) Clio, a sampling platform capable of collecting high-resolution vertical profile samples for biochemical and microbial measurements by large volume filtration of microbial particulate material, has overcome this challenge. Thus, this research project plans an interdisciplinary chemistry, biology, and engineering effort to test the hypothesis that certain chemical reactions, such as nitrite oxidation, could become limited by metal availability within the upper mesopelagic and that trace metal demands for nitrite-oxidizing bacteria may be increased under low oxygen conditions. Broader impacts of this study include the continued development and application of the Clio Biogeochemical AUV as a community resource by developing and testing its high-resolution and adaptive sampling capabilities. In addition, metaproteomic data will be deposited into the recently launched Ocean Protein Portal to allow oceanographers and the metals in biology community to examine the distribution of proteins and metalloenzymes in the ocean. Undergraduate students will be supported by this project at all three institutions, with an effort to recruit minority students. The proposed research will also be synergistic with the goals of early community-building efforts for a potential global scale microbial biogeochemistry program modeled after the success of the GEOTRACES program, provisionally called &quot;Biogeoscapes: Ocean metabolism and nutrient cycles on a changing planet&quot;.&lt;/p&gt;
&lt;p&gt;The proposed research project will test the following three hypotheses: (1) the microbial metalloenzyme distribution of the mesopelagic is spatially dynamic in response to environmental gradients in oxygen and trace metals, (2) nitrite oxidation in the Eastern Tropical Pacific Ocean can be limited by iron availability in the upper mesopelagic through an inability to complete biosynthesis of the microbial protein nitrite oxidoreductase, and (3) nitrite-oxidizing bacteria increase their metalloenzyme requirements at low oxygen, impacting the distribution of both dissolved and particulate metals within oxygen minimum zones. One of the challenges to characterizing the biogeochemistry of the mesopelagic ocean is an inability to effectively sample it. As a sampling platform, we will use the novel biogeochemical AUV Clio that enables high-resolution vertical profile samples for biochemical and microbial measurements by large volume filtration of microbial particulate material on a research expedition in the Eastern Tropical Pacific Ocean. Specific research activities will be orchestrated to test the hypotheses. Hypothesis 1 will be explored by comparison of hydrographic, microbial distributions, dissolved and particulate metal data, and metaproteomic results with profile samples collected by Clio. Hypothesis 2 will be tested by incubation experiments using 15NO2- oxidation rates on Clio-collected incubation samples. Hypothesis 3 will be tested by dividing targeted nitrite oxidoreductase protein copies by qPCR (quantitative polymerase chain reaction)-based nitrite oxidizing bacteria abundance (NOB) to determine if cellular copy number varies with oxygen distributions, and by metalloproteomic analyses of NOB cultures. The demonstration of trace metal limitation of remineralization processes, not just primary production, would transform our understanding of the role of metals in biogeochemical cycling and provide new ways with which to interpret sectional data of dissolved and particulate trace metal distributions in the ocean. The idea that oxygen may play a previously underappreciated role in controlling trace metals due not just to metals' physical chemistry, but also from changing biological demand, will improve our ability to predict trace metal distributions in the face of decreasing ocean oxygen content.&lt;/p&gt;
&lt;p&gt;This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.&lt;/p&gt;</gco:CharacterString>
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http://lod.bco-dmo.org/id/dataset-parameter/932266.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/932267.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/932268.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/932269.rdf
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	Units: unitless
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http://lod.bco-dmo.org/id/dataset-parameter/932270.rdf
	Name: DEPTH_STATION_TYPE
	Units: unitless
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http://lod.bco-dmo.org/id/dataset-parameter/932271.rdf
	Name: DEPTH_STATION_ORDER
	Units: unitless
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http://lod.bco-dmo.org/id/dataset-parameter/932272.rdf
	Name: DEPTH_STATION_PARAMETERS
	Units: meters
	Description: &lt;p&gt;The depth at which the vehicle was commanded to sample. Note for adaptive samples, this was the initialized depth, but may have been changed with the adaptive behavior. See actual depth column.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932273.rdf
	Name: BAY
	Units: unitless
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http://lod.bco-dmo.org/id/dataset-parameter/932274.rdf
	Name: SUPR_UNIT
	Units: unitless
	Description: &lt;p&gt;Which Supr unit took the sample (BUSHBABY or TIGERCLAW)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932275.rdf
	Name: SUPR_VALVE
	Units: unitless
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http://lod.bco-dmo.org/id/dataset-parameter/932276.rdf
	Name: SUPR_FILTER_INCUBATOR
	Units: unitless
	Description: &lt;p&gt;The filter number as installed in the SUPR sampler&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932277.rdf
	Name: FILTERS
	Units: unitless
	Description: &lt;p&gt;The type of filter installed&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932278.rdf
	Name: STOP_CONDITION_TIME_VOL_RATE
	Units: minutes (time), liters (volume), liters per minute (rate)
	Description: &lt;p&gt;What triggers sampling to stop: time, volume, or rate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932279.rdf
	Name: STOP_TIME
	Units: minutes
	Description: &lt;p&gt;The duration at which the SUPR sampler is set to stop pumping (unit = minutes) when time is used as the stop criterion.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932280.rdf
	Name: STOP_VOLUME
	Units: liters
	Description: &lt;p&gt;The volume at which the SUPR sampler is set to stop pumping (unit = liters) when volume is used as the stop criterion.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932281.rdf
	Name: STOP_RATE
	Units: liters per minute
	Description: &lt;p&gt;The minimum flow rate below which the SUPR sampler is set to stop pumping (unit = liters per minute) when flow rate is used as the stop criterion.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932282.rdf
	Name: INCUBATOR_PURGE_TIME
	Units: minute
	Description: &lt;p&gt;The duration set for the incubator to perform its purge sequence&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932283.rdf
	Name: INCUBATOR_FILL_TIME
	Units: minute
	Description: &lt;p&gt;The duration set for the incubator to fill&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932284.rdf
	Name: SAMPLE_START_TIME_UTC
	Units: UTC
	Description: &lt;p&gt;The time at which pumping started for the sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932285.rdf
	Name: ACTUAL_DEPTH_m
	Units: meters
	Description: &lt;p&gt;The actual depth the sample was taken at. This value is the average depth over time time period spent sampling.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932286.rdf
	Name: VOLUME_FILTERED_L
	Units: liters
	Description: &lt;p&gt;The amount of seawater pumped during the sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932287.rdf
	Name: TIME_SPENT_FILTERING_min
	Units: minutes
	Description: &lt;p&gt;Actual time spent pumping (should match stop time)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932288.rdf
	Name: VEHICLE_COMMENT
	Units: unitless
	Description: &lt;p&gt;Vehicle comment&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932289.rdf
	Name: SUBMERGE_TIME_UTC
	Units: unitless
	Description: &lt;p&gt;Time of last Iridium update before submerging&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932290.rdf
	Name: SUBMERGE_LAT
	Units: decimal degrees
	Description: &lt;p&gt;Latitude of last Iridium update before submerging&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932291.rdf
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	Units: decimal degrees
	Description: &lt;p&gt;Longitude of last Iridium update before submerging&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932292.rdf
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	Units: unitless
	Description: &lt;p&gt;Time of first Iridium update after surfacing (note that Clio could be on surface for a significant amount of time due to other ship operations before recovery)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932293.rdf
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	Units: decimal degrees
	Description: &lt;p&gt;Latitude of first Iridium update after surfacing (note that Clio could be on surface for a significant amount of time due to other ship operations before recovery)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932294.rdf
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	Units: decimal degrees
	Description: &lt;p&gt;Longitude of first Iridium update after surfacing (note that Clio could be on surface for a significant amount of time due to other ship operations before recovery)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/932295.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/932296.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/932297.rdf
	Name: NOTES
	Units: unitless
	Description: &lt;p&gt;Dive notes&lt;/p&gt; 
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