http://lod.bco-dmo.org/id/dataset/930084
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-06-21
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Flow cytometry, 16S rRNA gene amplicons, chlorophyll a, and surface seawater measurements taken between August 2017 to June 2019 Kāneʻohe Bay, Oʻahu, Hawaiʻi
2024-08-30
publication
2024-08-30
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-08-30
publication
https://doi.org/10.26008/1912/bco-dmo.930084.1
Michael S. Rappé
University of Hawaiʻi at Mānoa
principalInvestigator
Kelle C. Freel
University of Hawaiʻi at Mānoa
principalInvestigator
Yoshimi M. Rii
University of Hawaiʻi at Mānoa
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Tucker, S. J., Freel, K. C., Monaghan, E. A., Sullivan, C. E., Ramfelt, O., Rii, Y. M., Rappé, M. S. (2024) Flow cytometry, 16S rRNA gene amplicons, chlorophyll a, and surface seawater measurements taken between August 2017 to June 2019 Kāneʻohe Bay, Oʻahu, Hawaiʻi. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-08-30 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.930084.1 [access date]
Kāneʻohe Bay Time-series - microbial community Dataset Description: <p>Other Grants:<br />
* "National Science Foundation Graduate Research Fellowship Program" (Grant ID 1842402, National Science Foundation)<br />
* "NOAA Margaret A. Davidson Fellowship" (Grant ID NA20NOS4200123, National Oceanic and Atmospheric Administration)</p> Methods and Sampling: <p>Methods and sampling are reported in Tucker et al., (2021)</p>
<p>Sample collection and environmental parameters</p>
<p>Between August 2017 and June 2019, seawater was collected from a depth of 2 m at 10 sites in and around Kāneʻohe Bay, Oʻahu, Hawaiʻi, on a near-monthly basis (20 sampling events over 23 months). At each station, seawater samples for biogeochemical analyses and nucleic acids were collected, and&nbsp;<em>in situ</em>&nbsp;measurements of seawater temperature, pH, and salinity were made with a YSI 6,600 sonde (YSI Incorporated, Yellow Springs, OH, USA). Approximately one L of seawater was prefiltered using an 85 μm Nitex mesh and subsequently collected on a 25-mm diameter 0.1-μm pore-sized polyethersulfone (PES) membrane for nucleic acids (Supor-100, Pall Gelman Inc., Ann Arbor, MI, USA). The filters were submerged in DNA lysis buffer (Suzuki et al., 2001;&nbsp;Yeo et al., 2013) and stored at −80 °C until extraction.</p>
<p>Subsamples for chlorophyll&nbsp;<em>a</em>&nbsp;were collected by filtering 125 mL of seawater onto 25-mm diameter GF/F glass microfiber filters (Whatman, GE Healthcare Life Sciences, Chicago, IL, USA), and stored in aluminum foil at −80 °C until extraction in 100% acetone and subsequent measurement of fluorescence with a Turner 10AU fluorometer (Turner Designs, Sunnyvale, CA, USA) followed standard techniques (Welschmeyer, 1994). Seawater for cellular enumeration was preserved in two-mL aliquots in a final concentration of 0.95% (v:v) paraformaldehyde (Electron Microscopy Services, Hatfield, PA, USA) at −80 °C until analyzed&nbsp;<em>via</em>&nbsp;flow cytometry. Cellular enumeration of cyanobacterial picophytoplankton (marine&nbsp;<em>Synechococcus</em>&nbsp;and&nbsp;<em>Prochlorococcus</em>), eukaryotic picophytoplankton, and non-cyanobacterial (presumably heterotrophic) bacteria and archaea (hereafter referred to as heterotrophic bacteria) was performed on an EPICS ALTRA flow cytometer (Beckman Coulter Inc., Brea, CA, USA), following the method of Monger and Landry (Monger &amp; Landry, 1993).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538628 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538628
completed
Michael S. Rappé
University of Hawaiʻi at Mānoa
808-372-2394
University of Hawaii at Manoa - Hawaii Institute of Marine Biology 46-007 Lilipuna Rd
Kaneohe
HI
96744
USA
rappe@hawaii.edu
pointOfContact
Kelle C. Freel
University of Hawaiʻi at Mānoa
kfreel@hawaii.edu
pointOfContact
Yoshimi M. Rii
University of Hawaiʻi at Mānoa
shimi@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample_ID
collection_date
depth
env_broad_scale
env_local_scale
env_medium
geo_loc_name
latitude
longitude
Site_name
chlorophyll_a_ug_per_L
ph
salinity
Seawater_temperature
Prochlorococcus_cells_per_mL
Synechococcus_cells_per_mL
Heterotrophic_bacteria_cells_per_mL
Eukaryotic_picophytoplankton_cells_per_mL
Universal_Sample_ID
SRA_accession
study
bioproject_accession
biosample_accession
library_ID
library_strategy
library_source
library_selection
library_layout
platform
instrument_model
design_description
filetype
filename
filename2
Illumina MiSeq v2
EPICS ALTRA flow cytometer (Beckman Coulter Inc., Brea, CA, USA
Turner 10AU fluorometer (Turner Designs, Sunnyvale, CA, USA)
YSI 6,600 sonde (YSI Incorporated, Yellow Springs, OH, USA)
theme
None, User defined
sample identification
date
depth
sample description
region
latitude
longitude
site
chlorophyll a
pH
salinity
water temperature at measurement depth
prochlorococcus abundance
synechococcus abundance
cell_concentration
pico_euks
NCBI SRA run accession (SRR)
NCBI SRA study accession (SRP)
NCBI BioProject accession
NCBI BioSample accession
seq_descrip
instrument
instrument model
datatype
file_name
featureType
BCO-DMO Standard Parameters
Automated DNA Sequencer
Flow Cytometer
Turner Designs Fluorometer 10-AU
YSI Sonde 6-Series
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Population genomics and ecotypic divergence in the most dominant lineage of marine bacteria
https://rappelab.wordpress.com/marine-bacterioplankton/population-genomics-of-sar11-marine-bacteria/
Population genomics and ecotypic divergence in the most dominant lineage of marine bacteria
<p>In the upper water column of Earth's coastal and open oceans, roughly one million microscopic, single-celled bacteria inhabit each milliliter of seawater, where they play important roles in driving nutrient cycles and other processes that are vital to the habitability of these systems to other marine life. While some marine bacteria are similar to plants in that they use energy from the sun to transform the greenhouse gas carbon dioxide into living material and produce oxygen as a byproduct, other marine bacteria known as chemoheterotrophs are similar to humans and other animals in that they consume organic matter and oxygen, producing carbon dioxide as a consequence of their growth. Although they are limited in size and shape when observed under a microscope, genetic techniques such as DNA sequencing have revealed tremendous functional (i.e. what they are doing) and phylogenetic (i.e. how they are related) biodiversity in natural communities of marine bacteria. Despite this high genetic diversity, a single group of phylogenetically related chemoheterotrophic bacteria known as SAR11 can sometimes make up over 50% of the microscopic cells inhabiting seawater systems around the globe; it is considered one of the most abundant organisms on Earth and thus an important aspect of ocean ecology. While it is known that the SAR11 group consists of many distinct "types" that differ in abundance with location, depth and time, we know little about what genetically encoded features distinguish the different types, or how genetic characteristics are gained and lost within the group. The goal of this study is to use a genomics approach to understand the evolutionary processes that shape one of the most abundant groups of organisms on our planet, and to improve our theoretical understanding of the evolutionary processes that shape natural microbial biodiversity in general. This project will provide advanced, cross-disciplinary professional training for a postdoctoral scientist and a graduate student, and will increase the participation of underrepresented groups in scientific research by mentoring undergraduate students of native Hawaiian or Pacific Island ancestry in hands-on research and training. Results will be incorporated into a new university course offering on comparative genomics and microbial evolution. A culture collection of marine microorganisms will also be expanded and maintained, providing a valuable resource for other marine scientists.</p>
<p>This project will take advantage of recent advances in DNA sequencing technology and a high throughput extinction culturing approach in order to investigate the evolutionary characteristics of genomes from sympatric populations of the globally important SAR11 marine bacterial lineage. The major objectives of this project are to understand the forces that shape genomic diversity in large bacterial populations such as SAR11, and to determine the nature by which this diversity is reflected in functional differences between populations, as inferred from genomics. SAR11 cells will be isolated from similar ecosystems in the tropical North and South Pacific, as well as the coastal ocean of the subpolar North Pacific, in order to investigate the effect of geographic distance versus habitat similarity on the population genetics of free-living, planktonic marine bacteria. By opening a unique genomic window that encompasses SAR11 lineages of varying degrees of genetic divergence simultaneously, this study will facilitate the investigation of evolutionary dynamics that spans a continuum between macro- and microevolutionary processes. Quantitative information regarding the mechanisms by which genetic diversity is generated, propagated, and removed from native SAR11 populations will also help efforts to model the fate of SAR11 and other large marine bacterioplankton populations in the face of predicted climate-induced changes to the global ocean.</p>
Pelagibacteromics
largerWorkCitation
project
eng; USA
oceans
-157.83585
-157.7663
21.43635
21.526
2017-08-23
2019-06-14
Moorea, Oahu, and Gulf of Alaska
0
BCO-DMO catalogue of parameters from Flow cytometry, 16S rRNA gene amplicons, chlorophyll a, and surface seawater measurements taken between August 2017 to June 2019 Kāneʻohe Bay, Oʻahu, Hawaiʻi
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/930125.rdf
Name: Sample_ID
Units: unitless
Description: <p>Sample ID. Sample ID is composed of the station ID abbreviation and a uniqe sample ID #. In most cases a month abbreviation is also included.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930126.rdf
Name: collection_date
Units: unitless
Description: <p>Collection date.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930127.rdf
Name: depth
Units: m
Description: <p>Depth of sample collection.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930128.rdf
Name: env_broad_scale
Units: unitless
Description: <p>Environment description, broad.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930129.rdf
Name: env_local_scale
Units: unitless
Description: <p>Environment description, local. Defined by analyses included in associated publication.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930130.rdf
Name: env_medium
Units: unitless
Description: <p>Source of sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930131.rdf
Name: geo_loc_name
Units: unitless
Description: <p>Geographic location of sampling.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930132.rdf
Name: latitude
Units: decimal degrees
Description: <p>Station latitude, south is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/930133.rdf
Name: longitude
Units: decimal degrees
Description: <p>Station longitude, west is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/930134.rdf
Name: Site_name
Units: unitless
Description: <p>Site ID for collection.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930135.rdf
Name: chlorophyll_a_ug_per_L
Units: micrograms per Liter
Description: <p>Extracted chlorophyll a concentrations from surface seawater.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930136.rdf
Name: ph
Units: no unit
Description: <p>pH of surface seawater in situ.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930137.rdf
Name: salinity
Units: ppt
Description: <p>Salinity of surface seawater in situ.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930138.rdf
Name: Seawater_temperature
Units: degrees Celsius (°C)
Description: <p>Temperature of surface seawater in situ.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930139.rdf
Name: Prochlorococcus_cells_per_mL
Units: cells per mL
Description: <p>Surface seawater cellular abundances of Prochlorocococcus cells counted on EPICS ALTRA flow cytometer.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930140.rdf
Name: Synechococcus_cells_per_mL
Units: cells per mL
Description: <p>Surface seawater cellular abundances of Synechococcus cells counted on EPICS ALTRA flow cytometer.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930141.rdf
Name: Heterotrophic_bacteria_cells_per_mL
Units: cells per mL
Description: <p>Surface seawater cellular abundances of non-cyanobacterial (presumably heterotrophic) bacteria and archaea (referred to as heterotrophic bacteria) counted on EPICS ALTRA flow cytometer.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930142.rdf
Name: Eukaryotic_picophytoplankton_cells_per_mL
Units: cells per mL
Description: <p>Surface seawater cellular abundances of photosynthetic picoeukaryotes counted on EPICS ALTRA flow cytometer.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930143.rdf
Name: Universal_Sample_ID
Units: unitless
Description: <p>Universal Sample ID. Universal Sample ID is used to connect multiple sample types within the Kāneʻohe Bay Time-series. It is composed of the Project ID "KBT", the date (mm.dd.yyyy), and Station ID.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930144.rdf
Name: SRA_accession
Units: unitless
Description: <p>NCBI SRA Accession for amplicon data.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930145.rdf
Name: study
Units: unitless
Description: <p>NCBI Study ID.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930146.rdf
Name: bioproject_accession
Units: unitless
Description: <p>NCBI Bioproject Accession for amplicon data.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930147.rdf
Name: biosample_accession
Units: unitless
Description: <p>NCBI Biosample Accession.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930148.rdf
Name: library_ID
Units: unitless
Description: <p>Unique barcode sample identifier for amplicon sequencing.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930149.rdf
Name: library_strategy
Units: unitless
Description: <p>Sequencing library type.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930150.rdf
Name: library_source
Units: unitless
Description: <p>Source of sequencing library.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930151.rdf
Name: library_selection
Units: unitless
Description: <p>Mode of library creation.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930152.rdf
Name: library_layout
Units: unitless
Description: <p>Single or paired end sequencing reads.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930153.rdf
Name: platform
Units: unitless
Description: <p>Platform used for library creation.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930154.rdf
Name: instrument_model
Units: unitless
Description: <p>Sequencer model.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930155.rdf
Name: design_description
Units: unitless
Description: <p>Description of ampicon library.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930156.rdf
Name: filetype
Units: unitless
Description: <p>File type.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930157.rdf
Name: filename
Units: unitless
Description: <p>Forward reads file name.</p>
http://lod.bco-dmo.org/id/dataset-parameter/930158.rdf
Name: filename2
Units: unitless
Description: <p>Reverse reads file name.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
102318
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/858ca648-3054-4720-86c9-debdb52fba3c/content
download
https://doi.org/10.26008/1912/bco-dmo.930084.1
download
onLine
dataset
<p>Methods and sampling are reported in Tucker et al., (2021)</p>
<p>Sample collection and environmental parameters</p>
<p>Between August 2017 and June 2019, seawater was collected from a depth of 2 m at 10 sites in and around Kāneʻohe Bay, Oʻahu, Hawaiʻi, on a near-monthly basis (20 sampling events over 23 months). At each station, seawater samples for biogeochemical analyses and nucleic acids were collected, and&nbsp;<em>in situ</em>&nbsp;measurements of seawater temperature, pH, and salinity were made with a YSI 6,600 sonde (YSI Incorporated, Yellow Springs, OH, USA). Approximately one L of seawater was prefiltered using an 85 μm Nitex mesh and subsequently collected on a 25-mm diameter 0.1-μm pore-sized polyethersulfone (PES) membrane for nucleic acids (Supor-100, Pall Gelman Inc., Ann Arbor, MI, USA). The filters were submerged in DNA lysis buffer (Suzuki et al., 2001;&nbsp;Yeo et al., 2013) and stored at −80 °C until extraction.</p>
<p>Subsamples for chlorophyll&nbsp;<em>a</em>&nbsp;were collected by filtering 125 mL of seawater onto 25-mm diameter GF/F glass microfiber filters (Whatman, GE Healthcare Life Sciences, Chicago, IL, USA), and stored in aluminum foil at −80 °C until extraction in 100% acetone and subsequent measurement of fluorescence with a Turner 10AU fluorometer (Turner Designs, Sunnyvale, CA, USA) followed standard techniques (Welschmeyer, 1994). Seawater for cellular enumeration was preserved in two-mL aliquots in a final concentration of 0.95% (v:v) paraformaldehyde (Electron Microscopy Services, Hatfield, PA, USA) at −80 °C until analyzed&nbsp;<em>via</em>&nbsp;flow cytometry. Cellular enumeration of cyanobacterial picophytoplankton (marine&nbsp;<em>Synechococcus</em>&nbsp;and&nbsp;<em>Prochlorococcus</em>), eukaryotic picophytoplankton, and non-cyanobacterial (presumably heterotrophic) bacteria and archaea (hereafter referred to as heterotrophic bacteria) was performed on an EPICS ALTRA flow cytometer (Beckman Coulter Inc., Brea, CA, USA), following the method of Monger and Landry (Monger &amp; Landry, 1993).</p>
Specified by the Principal Investigator(s)
<h3>DNA extraction and sequencing</h3>
<p>Genomic DNA was extracted using a Qiagen Blood and Tissue Kit (Qiagen Inc., Valencia, CA, USA) with modifications (Becker, Brandon &amp; Rappé, 2007). For each sample, 16S rRNA gene fragments were amplified by polymerase chain reaction using a dual-index sequencing strategy where barcoded universal primers 515-Y-F and 926R (Parada, Needham &amp; Fuhrman, 2016) are complete with Illumina sequencing adapters, barcode, and index. The 25 µL reactions included 13 µL H<sub>2</sub>O, 0.5 µL each of forward and reverse primer at 0.2 µm final concentration, one µL gDNA (0.5 ng), and 10 µL 1 × 5PRIME Hot Master Mix (0.5 U&nbsp;<em>Taq</em>&nbsp;DNA polymerase, 45 mm KCl, 2.5 mm Mg<sup>2+</sup>, 200 μm dNTPs) (Quantabio, Beverly, MA, USA). PCR conditions included an initial denaturation at 95 °C for 2 min followed by 30 cycles of 95 °C for 45 s, 50 °C for 45 s, and 68 °C for 90 s, and a final 5 min extension at 68 °C. PCR products were inspected on a 1.5% agarose gel and quantified using the Qubit dsDNA HS Kit (Qubit 2.0, Life Technologies, Foster City, CA, USA). PCR products were normalized to 240 ng each, pooled, and purified using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany). Pooled libraries were then sequenced on an Illumina MiSeq v2 250 bp paired-end run at the Oregon State University Center for Genome Research &amp; Biocomputing.</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
* No data value NA set to blank
* Split latitude and longitude value into own column.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Illumina MiSeq v2
Illumina MiSeq v2
PI Supplied Instrument Name: Illumina MiSeq v2 Instrument Name: Automated DNA Sequencer Instrument Short Name:Automated Sequencer Instrument Description: A DNA sequencer is an instrument that determines the order of deoxynucleotides in deoxyribonucleic acid sequences.
EPICS ALTRA flow cytometer (Beckman Coulter Inc., Brea, CA, USA
EPICS ALTRA flow cytometer (Beckman Coulter Inc., Brea, CA, USA
PI Supplied Instrument Name: EPICS ALTRA flow cytometer (Beckman Coulter Inc., Brea, CA, USA Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
Turner 10AU fluorometer (Turner Designs, Sunnyvale, CA, USA)
Turner 10AU fluorometer (Turner Designs, Sunnyvale, CA, USA)
PI Supplied Instrument Name: Turner 10AU fluorometer (Turner Designs, Sunnyvale, CA, USA) Instrument Name: Turner Designs Fluorometer 10-AU Instrument Short Name:Turner Fluorometer 10-AU Instrument Description: The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/
YSI 6,600 sonde (YSI Incorporated, Yellow Springs, OH, USA)
YSI 6,600 sonde (YSI Incorporated, Yellow Springs, OH, USA)
PI Supplied Instrument Name: YSI 6,600 sonde (YSI Incorporated, Yellow Springs, OH, USA) Instrument Name: YSI Sonde 6-Series Instrument Short Name:YSI Sonde 6-Series Instrument Description: YSI 6-Series water quality sondes and sensors are instruments for environmental monitoring and long-term deployments. YSI datasondes accept multiple water quality sensors (i.e., they are multiparameter sondes). Sondes can measure temperature, conductivity, dissolved oxygen, depth, turbidity, and other water quality parameters. The 6-Series includes several models. More from YSI. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0737/