http://lod.bco-dmo.org/id/dataset/931765
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-07-05
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Phosphate Profile Data Comparison between Programmable Flow Injection (pFI) and AA3 Autoanalyzer Methods from R/V Kilo Moana KM2210 in the North Pacific Subtropical Gyre during September 2022 (pFI-SI-LOV project)
2024-07-05
publication
2024-07-05
revision
BCO-DMO Linked Data URI
2024-07-05
creation
http://lod.bco-dmo.org/id/dataset/931765
Maxime Grand
Moss Landing Marine Laboratories
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Grand, M. (2024) Phosphate Profile Data Comparison between Programmable Flow Injection (pFI) and AA3 Autoanalyzer Methods from R/V Kilo Moana KM2210 in the North Pacific Subtropical Gyre during September 2022 (pFI-SI-LOV project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-07-05 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/931765 [access date]
Phosphate profile data comparison between programmable Flow Injection (PFI) and AA3 Autoanalyzer methods Dataset Description: Methods and Sampling: <p>We collected unfiltered seawater samples directly from the Niskin bottles. One set was analyzed in triplicate for phosphate using the pFI analyzer calibrated with MQ standards (0-3&nbsp;µmol L<sup>-1</sup>). The duplicate samples were frozen, shipped to Scripps Institution of Oceanography (SIO) Ocean Data Facility chemistry laboratory on dry ice and analyzed at SIO’s lab using an AA3 autoanalyzer.&nbsp;</p>
<p>The pFI analyzer was calibrated with working phosphate standards prepared in MQ water&nbsp;(0-3&nbsp;µmol L<sup>-1</sup>). To calculate phosphate concentrations at each depth, we took the mean of the baseline corrected absorbance at 880 nm minus the reference absorbance at 1050 nm. Phosphate concentrations were then calculated using the slope and intercept of the MQ calibration curve.&nbsp;</p>
<p>pFI LOD: 0.06 umol/L</p>
<p>pFI precision: 4.1%</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1924539 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1924539
onGoing
Maxime Grand
Moss Landing Marine Laboratories
8317714447
8272 Moss Landing Rd
Moss Landing
CA
950399647
US
mgrand@mlml.calstate.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Latitude
Longitude
ISO_DateTime_UTC
Date_UTC
Time_UTC
Depth
pFI_PO4
pFI_flag
SIO_PO4
SIO_flag
pFI analyzer (miniSIA2, GlobalFIA, USA)
SIO's AA3 analyzer (Seal Analytical, USA)
VIS-NIR Flame spectrophotometer (Ocean Insight, USA)
theme
None, User defined
latitude
longitude
Date and time
date
time of day
depth
phosphate
quality flag
featureType
BCO-DMO Standard Parameters
Flow Injection Analyzer
Flow Injection Analyzer
Spectrometer
instrument
BCO-DMO Standard Instruments
KM2210
service
Deployment Activity
North Pacific subtropical gyre
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Developing Automated Nutrient and Trace Metal Methodology using Programmable Flow Injection
https://osprey.bco-dmo.org/project/815753
Collaborative Research: Developing Automated Nutrient and Trace Metal Methodology using Programmable Flow Injection
<p>NSF Award Abstract:</p>
<p>Progress and discovery in the understanding of the chemical processes that regulate the growth of phytoplankton in the marine environment is limited by the ability of oceanographers to measure nutrients and trace metals at relevant spatial and temporal scales. This project aims to develop a new generation of autonomous and highly sensitive nutrient and trace metal analyzers for shipboard use and deployment at coastal monitoring stations, with potential for incorporation into existing autonomous observing platforms (e.g., gliders, autonomous underwater vehicles). Such a development will generate new insights into nutrient dynamics at a range of spatial and temporal scales and provide a new capability to obtain high resolution data sets for nutrients and trace metals in remote areas and environments where sample volumes are limited (e.g., brines, pore waters).</p>
<p>This project will develop novel automated methods for nutrients (phosphate and silicate) and trace metals (aluminium, manganese) using a technology called programmable Flow Injection (pFI). pFI is a microfluidic technique, which allows to automate conventional wet chemical analysis using microliter volumes of sample and reagents while significantly reducing the generation waste. In order to bring low levels of trace metals and nutrients that characterize remote ocean regions into the analytical window of pFI, concentration techniques will also be developed and coupled to spectrophotometric and fluorescence detection. The developed methodologies will be intercalibrated with standard oceanographic methods by participating in a GO-SHIP research cruise and a Hawaii Ocean Time-series cruise of opportunity. The long-term, unattended operation of the pFI analyzers will be evaluated at the Central California Ocean Observing System (CenCOOS) Moss Landing Shore station, where autonomous pFI analyzers will be deployed to undertake a year-long, hourly time series of phosphate and silicate. The data obtained from the shore station will be made publicly available and will complement existing monitoring data from this location, illuminating the connection between deep ocean nutrient dynamics and biological activity in coastal upwelling systems. The newly developed pFI analyzers will be assembled using commercially available components and open source software to facilitate uptake of this new methodology by the chemical oceanography community at large. This project will also support the training and exchange of at least two graduate students at the University of Hawaii at Mānoa and the Moss Landing Marine Laboratories, who will represent the next generation of specialists in the development and application of pFI methodologies to chemical oceanography.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
pFI-SI-LOV
largerWorkCitation
project
eng; USA
oceans
North Pacific subtropical gyre
158
158
22.75
22.75
2022-09-01
2022-09-01
0
BCO-DMO catalogue of parameters from Phosphate Profile Data Comparison between Programmable Flow Injection (pFI) and AA3 Autoanalyzer Methods from R/V Kilo Moana KM2210 in the North Pacific Subtropical Gyre during September 2022 (pFI-SI-LOV project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/931828.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Water sample latitude in decimal degrees; a positive value indicates a Northern coordinate.</p>
http://lod.bco-dmo.org/id/dataset-parameter/931829.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Water sample longitude in decimal degrees; a negative value indicates a Western coordinate.</p>
http://lod.bco-dmo.org/id/dataset-parameter/931830.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Water sample date time in UTC.</p>
http://lod.bco-dmo.org/id/dataset-parameter/931831.rdf
Name: Date_UTC
Units: unitless
Description: <p>Water sample date in UTC.</p>
http://lod.bco-dmo.org/id/dataset-parameter/931832.rdf
Name: Time_UTC
Units: unitless
Description: <p>Water sample time in UTC.</p>
http://lod.bco-dmo.org/id/dataset-parameter/931833.rdf
Name: Depth
Units: meters (m)
Description: <p>Water sample depth in the water column in meters.</p>
http://lod.bco-dmo.org/id/dataset-parameter/931834.rdf
Name: pFI_PO4
Units: umol/L
Description: <p>Phosphate concentration from pFI analyzer.</p>
http://lod.bco-dmo.org/id/dataset-parameter/931835.rdf
Name: pFI_flag
Units: unitless
Description: <p>pFI flag. 1 = good, 2 = bad, 3 = below detection limit.</p>
http://lod.bco-dmo.org/id/dataset-parameter/931836.rdf
Name: SIO_PO4
Units: umol/L
Description: <p>Phosphate concentration from AA3 analyzer (SIO).</p>
http://lod.bco-dmo.org/id/dataset-parameter/931837.rdf
Name: SIO_flag
Units: unitless
Description: <p>SIO flag. 1 = good, 2 = bad, 3 = below detection limit.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/931765/data/download
download
onLine
dataset
<p>We collected unfiltered seawater samples directly from the Niskin bottles. One set was analyzed in triplicate for phosphate using the pFI analyzer calibrated with MQ standards (0-3&nbsp;µmol L<sup>-1</sup>). The duplicate samples were frozen, shipped to Scripps Institution of Oceanography (SIO) Ocean Data Facility chemistry laboratory on dry ice and analyzed at SIO’s lab using an AA3 autoanalyzer.&nbsp;</p>
<p>The pFI analyzer was calibrated with working phosphate standards prepared in MQ water&nbsp;(0-3&nbsp;µmol L<sup>-1</sup>). To calculate phosphate concentrations at each depth, we took the mean of the baseline corrected absorbance at 880 nm minus the reference absorbance at 1050 nm. Phosphate concentrations were then calculated using the slope and intercept of the MQ calibration curve.&nbsp;</p>
<p>pFI LOD: 0.06 umol/L</p>
<p>pFI precision: 4.1%</p>
Specified by the Principal Investigator(s)
<p><u>Quality flags represented in the primary data file</u></p>
<ul>
<li>1 = good</li>
<li>2 = bad*</li>
<li>3 = below detection limit</li>
</ul>
<p><span style="color:rgb(36, 36, 36); font-size:15px">Data were flagged</span><span style="color:rgb(36, 36, 36); font-size:15px">&nbsp;when the data were inconsistent with adjacent depths and visual observation of the absorbance spectra confirmed an issue during the analysis.&nbsp;</span></p>
Specified by the Principal Investigator(s)
- Units and format details removed from parameter names
- Special characters removed from parameter names
- Spaces in character names replaced with underscores ("_")
- Latitude and longitude values rounded to 6 degrees of precision
- Date format converted from %m%d%y to %Y-%m-%d
- A datetime column was added to the data file by merging the original separate date and time columns (the original date and time columns have also been retained)
- Time format converted from %H%M%S to %H:%M:%S
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
pFI analyzer (miniSIA2, GlobalFIA, USA)
pFI analyzer (miniSIA2, GlobalFIA, USA)
PI Supplied Instrument Name: pFI analyzer (miniSIA2, GlobalFIA, USA) PI Supplied Instrument Description:One set of samples was analyzed using a pFI analyzer (miniSIA2, GlobalFIA, USA). This instrument was furnished with two High Flow milliGAT pumps with stainless steel heads, a plexiglass 8-port Lab-On-Valve, a 20 cm Long Path Linear Flow cell equipped with a Tungsten-Halogen lamp and a VIS-NIR Flame spectrophotometer (Ocean Insight, USA). Instrument Name: Flow Injection Analyzer Instrument Short Name:FIA Instrument Description: An instrument that performs flow injection analysis. Flow injection analysis (FIA) is an approach to chemical analysis that is accomplished by injecting a plug of sample into a flowing carrier stream. FIA is an automated method in which a sample is injected into a continuous flow of a carrier solution that mixes with other continuously flowing solutions before reaching a detector. Precision is dramatically increased when FIA is used instead of manual injections and as a result very specific FIA systems have been developed for a wide array of analytical techniques. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB36/
SIO's AA3 analyzer (Seal Analytical, USA)
SIO's AA3 analyzer (Seal Analytical, USA)
PI Supplied Instrument Name: SIO's AA3 analyzer (Seal Analytical, USA) PI Supplied Instrument Description:Samples not analyzed with the mini SIA2 pFI analyzer were analyzed using a SIO's AA3 analyzer (Seal Analytical, USA) following the vendor's established GO-SHIP protocols. Instrument Name: Flow Injection Analyzer Instrument Short Name:FIA Instrument Description: An instrument that performs flow injection analysis. Flow injection analysis (FIA) is an approach to chemical analysis that is accomplished by injecting a plug of sample into a flowing carrier stream. FIA is an automated method in which a sample is injected into a continuous flow of a carrier solution that mixes with other continuously flowing solutions before reaching a detector. Precision is dramatically increased when FIA is used instead of manual injections and as a result very specific FIA systems have been developed for a wide array of analytical techniques. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB36/
VIS-NIR Flame spectrophotometer (Ocean Insight, USA)
VIS-NIR Flame spectrophotometer (Ocean Insight, USA)
PI Supplied Instrument Name: VIS-NIR Flame spectrophotometer (Ocean Insight, USA) PI Supplied Instrument Description:One set of samples was analyzed using a pFI analyzer (miniSIA2, GlobalFIA, USA). This instrument was furnished with two High Flow milliGAT pumps with stainless steel heads, a plexiglass 8-port Lab-On-Valve, a 20 cm Long Path Linear Flow cell equipped with a Tungsten-Halogen lamp and a VIS-NIR Flame spectrophotometer (Ocean Insight, USA). Instrument Name: Spectrometer Instrument Short Name:Spectrometer Instrument Description: A spectrometer is an optical instrument used to measure properties of light over a specific portion of the electromagnetic spectrum. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0460/
Cruise: KM2210
KM2210
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM2210
Daniel W. Sadler
University of Hawai'i
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel