http://lod.bco-dmo.org/id/dataset/933832
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-07-26
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Lesion frequencies and sizes after fish feces treatment on coral samples collected on the north shore of Mo’orea, French Polynesia, Oct 2020 to Jun 2021
2024-09-24
publication
2024-09-24
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-11-20
publication
https://doi.org/10.26008/1912/bco-dmo.933832.1
Adrienne M.S. Correa
Rice University
principalInvestigator
Carsten G.B. Grupstra
Rice University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Correa, A. M., Grupstra, C. (2024) Lesion frequencies and sizes after fish feces treatment on coral samples collected on the north shore of Mo’orea, French Polynesia, Oct 2020 to Jun 2021. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-09-24 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.933832.1 [access date]
Coral lesion frequencies and sizes after fish feces treatment Dataset Description: Methods and Sampling: <p>A feces addition experiment was conducted three times (Final N = 11 replicates) using fragments of multiple species of <em>Pocillopora</em>&nbsp;in Moorea, French Polynesia. An initial experimental iteration was performed in October 2020 (four replicates); two additional iterations took place in June 2021 (5 replicates each).&nbsp;Coral colonies were collected from the fore reef on the north shore of Mo'orea on Scuba at 4-8 m depth and immediately returned to the research station and placed into flow-through seawater tables.&nbsp;After 24&nbsp;h, all corals were cut into five ~10 cm long fragments and left to acclimate for 48 hours, and any macrosymbionts (e.g., Trapezia&nbsp;crabs, shrimp) were removed. All fragments were transferred to glass jars containing 500&nbsp;ml of 0.2 μm-filtered (sterile) seawater with bubblers and air stones to promote aeration and water circulation, and photographed under a dissection microscope with 3.5-180X zoom (AmScope SM-1TSZZ-144S-10M).&nbsp;</p>
<p>We then blindly assigned each jar containing a coral fragment to one of the following five treatments (such that one fragment from each coral colony was assigned to each treatment, 4 replicate colonies x 5 treatments = 20 coral fragments in separate jars): fresh feces from a corallivorous butterflyfish (FC); fresh feces from a grazer/detritivore (FG); sterilized feces from a corallivorous butterflyfish (SC); sterilized feces from a grazer/detritivore (SG); no-feces control (C). For the fresh feces treatments (FC, FG), we applied 100 µl of fresh feces isolated from the hindgut of the butterflyfish&nbsp;Chaetodon ornatissimus&nbsp;(FC) or the grazer/detritivore&nbsp;Ctenochaetus striatus&nbsp;(FG) directly onto each coral fragment. Fish were collected on the north shore of Mo'orea using a Hawaiian sling and immediately transported on ice to the laboratory. Feces were isolated from the hindgut using sterile tools by making an incision from the anus to the pelvic fin and removing the intestinal tract; feces were then squeezed from the hindgut into sterile collection tubes using sterile tweezers and pipetted onto coral fragments using 1&nbsp;ml filter tips that were modified to widen the tip opening using a sterilized razor blade. For the sterilized feces treatments (SC, SG), fecal pellets were sterilized in a pressure cooker for 40 minutes at 120 °C and then applied in the same manner as fresh feces. The fresh feces used in the experiment were subsampled for DNA extractions by preservation in DNA/RNA Shield (Zymo Research, CA). No manipulation was conducted on the no-feces control fragments. The experiment ran for ~22 hours; this treatment duration is reflective of time periods over which corals may sometimes be in direct contact with fish feces&nbsp;in situ&nbsp;(up to 48 hours;&nbsp;Ezzat et&nbsp;al., 2019;&nbsp;Ezzat et&nbsp;al., 2021).&nbsp;</p>
<p>Minor modifications were incorporated into the design of the second and third experimental iterations: for these iterations, five replicate colonies were used instead of four. Additionally, fecal treatments in these iterations were composed of feces from two individuals per fish species that were mixed prior to application (instead of using feces from a single fish individual per treatment as in the first experimental iteration).</p>
<p>To test how microbial communities in fish feces affected coral health, we quantified the frequencies and sizes of coral lesions caused by fecal treatments (in addition to measuring the photosynthetic efficiency of each fragment). In brief, fecal pellets were removed from each fragment and photographs were taken using a dissection microscope.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2145472 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2145472
completed
Adrienne M.S. Correa
Rice University
amsc@berkeley.edu
pointOfContact
Carsten G.B. Grupstra
Rice University
777 Glades Road
Boca Raton
FL
33431
USA
cgrupstra@fau.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample_ID
Collection_Date
Experiment
Treatment
Colony
Replicate
Health
Dead_Polyps
Imaging PAM maxi (k-6) (Walz, Germany)
Dissection scope (AmScope SM-1TSZZ-144S-10M)
theme
None, User defined
sample identification
date
experiment id
treatment
number
replicate
mortality
count
featureType
BCO-DMO Standard Parameters
Fluorometer
Microscope - Optical
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
CAREER: Testing the effects of predator-derived feces on host symbiont acquisition and health
https://osprey.bco-dmo.org/project/868967
CAREER: Testing the effects of predator-derived feces on host symbiont acquisition and health
<p><em>NSF Award Abstract:</em></p>
<p>Climate change and local-scale anthropogenic stressors are degrading coral reefs across the globe. When conditions become too stressful on reefs, corals can lose beneficial microbial symbionts (e.g., dinoflagellates in the family Symbiodiniaceae) that live in their tissues via a process called ?bleaching?. Although Symbiodiniaceae play key roles in the health of coral colonies, we know little about the processes that make symbionts available in the environment to prospective host corals. This research tests the extent to which the feces of coral-eating fish, which contain live Symbiodiniaceae, facilitate symbiont acquisition by corals in their early life stages. It will generate seminal knowledge on how corallivore feces impact coral symbioses and health, and will assess the ecological importance of corallivorous fishes as drivers of coral symbiont assemblages. This research also test the extent to which corallivore feces are a source of food and nutrients that impact coral health; this has particular relevance to the survival and recovery of bleached adult corals. This research can ultimately inform intervention strategies to support reef resilience and mitigate reef degradation. Results from this project will be communicated widely in scientific arenas, in undergraduate education programs, and to the public via multimedia content and outreach. The Houston Independent School District (HISD, Houston, TX) is the nation?s 7th largest public school system. This work will also support economically disadvantaged and first-generation undergraduate students in pursuing STEM majors and careers through multi-year research experiences.</p>
<p>Symbioses between foundation species (e.g., corals, sponges, trees) and microbiota (e.g., microeukaryotes, bacteria) underpin the biodiversity, productivity, and stability of ecosystems. Consumers, such as predators and herbivores, shape communities of these foundation species through trophic interactions. For instance, grazers contribute to the maintenance of coral dominance on reefs via consumption of macroalgal competitors. However, the indirect effects of other consumers on foundation species are rarely examined. Few studies have tested how consumers affect microbiota assembly in corals, even though coral symbionts (e.g., dinoflagellates in the family Symbiodiniaceae) play key roles in reef function and persistence. Corallivorous (coral-eating) fishes were recently demonstrated to egest large quantities of live Symbiodiniaceae cells as they swim across reefs. This research is testing the hypothesis that corallivore feces promote coral dominance on reefs by supporting coral acquisition of key symbionts and nutrients. The following research objectives will be accomplished: (1) to quantify the contribution of corallivorous fish feces to coral symbiont acquisition; and (2) to test the extent to which corallivorous fish feces influence coral health and recovery from thermal stress. Reefs are being degraded globally due to climate-change induced bleaching and associated mortality. This project is teasing apart the extent to which nutrients and/or live symbionts associated with corallivore feces contribute to the resilience of bleached corals under ambient and heat stress conditions. The research is tightly integrated with two education objectives: (1) to organize a Research Experience for Teachers (RET) program in which rigorous learning modules that high school teachers can incorporate into their Environmental Systems course offerings are developed and tested; and (2) to provide undergraduate students with a multi-year research experience through a partnership with the Rice Emerging Scholars Program (RESP).</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Fish transmit microbes
largerWorkCitation
project
eng; USA
biota
oceans
-149.849444
-149.827222
-17.483056
-17.474444
2020-10-01
2021-06-30
0
BCO-DMO catalogue of parameters from Lesion frequencies and sizes after fish feces treatment on coral samples collected on the north shore of Mo’orea, French Polynesia, Oct 2020 to Jun 2021
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/938231.rdf
Name: Sample_ID
Units: unitless
Description: <p>Unique identifer of samples and treatment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/938232.rdf
Name: Collection_Date
Units: unitless
Description: <p>Date of sampling as year and month.</p>
http://lod.bco-dmo.org/id/dataset-parameter/938235.rdf
Name: Experiment
Units: unitless
Description: <p>Iteration of the experiment, graco1 (2020), graco2 (2021), or graco3 (2021).</p>
http://lod.bco-dmo.org/id/dataset-parameter/938236.rdf
Name: Treatment
Units: unitless
Description: <p>Treatment name; C= control, FC= fresh corallivore feces, FG= fresh grazer feces, SC= sterile corallivore feces, SG=sterile grazer feces.</p>
http://lod.bco-dmo.org/id/dataset-parameter/938237.rdf
Name: Colony
Units: unitless
Description: <p>Coral colony number; note that these are unique per year (graco1 = 2020, graco2 and graco3= 2021).</p>
http://lod.bco-dmo.org/id/dataset-parameter/938238.rdf
Name: Replicate
Units: unitless
Description: <p>Replicate; combination of treatment name and colony number.</p>
http://lod.bco-dmo.org/id/dataset-parameter/938239.rdf
Name: Health
Units: unitless
Description: <p>Health state of the fragment after removal of the fecal pellet; healthy (there was no lesion), lesion (there was one lesion, i.e., a patch where coral tissue had died), or dead (the entire fragment died).</p>
http://lod.bco-dmo.org/id/dataset-parameter/938240.rdf
Name: Dead_Polyps
Units: unitless
Description: <p>Number of polyps that died due to application of fecal treatment. If a replicate was "healthy" or "dead" in the above file, then it is not included in this file.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2559
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/d7702666-0b7a-4b4c-94f9-5ccd23d717c6/content
download
https://doi.org/10.26008/1912/bco-dmo.933832.1
download
onLine
dataset
<p>A feces addition experiment was conducted three times (Final N = 11 replicates) using fragments of multiple species of <em>Pocillopora</em>&nbsp;in Moorea, French Polynesia. An initial experimental iteration was performed in October 2020 (four replicates); two additional iterations took place in June 2021 (5 replicates each).&nbsp;Coral colonies were collected from the fore reef on the north shore of Mo'orea on Scuba at 4-8 m depth and immediately returned to the research station and placed into flow-through seawater tables.&nbsp;After 24&nbsp;h, all corals were cut into five ~10 cm long fragments and left to acclimate for 48 hours, and any macrosymbionts (e.g., Trapezia&nbsp;crabs, shrimp) were removed. All fragments were transferred to glass jars containing 500&nbsp;ml of 0.2 μm-filtered (sterile) seawater with bubblers and air stones to promote aeration and water circulation, and photographed under a dissection microscope with 3.5-180X zoom (AmScope SM-1TSZZ-144S-10M).&nbsp;</p>
<p>We then blindly assigned each jar containing a coral fragment to one of the following five treatments (such that one fragment from each coral colony was assigned to each treatment, 4 replicate colonies x 5 treatments = 20 coral fragments in separate jars): fresh feces from a corallivorous butterflyfish (FC); fresh feces from a grazer/detritivore (FG); sterilized feces from a corallivorous butterflyfish (SC); sterilized feces from a grazer/detritivore (SG); no-feces control (C). For the fresh feces treatments (FC, FG), we applied 100 µl of fresh feces isolated from the hindgut of the butterflyfish&nbsp;Chaetodon ornatissimus&nbsp;(FC) or the grazer/detritivore&nbsp;Ctenochaetus striatus&nbsp;(FG) directly onto each coral fragment. Fish were collected on the north shore of Mo'orea using a Hawaiian sling and immediately transported on ice to the laboratory. Feces were isolated from the hindgut using sterile tools by making an incision from the anus to the pelvic fin and removing the intestinal tract; feces were then squeezed from the hindgut into sterile collection tubes using sterile tweezers and pipetted onto coral fragments using 1&nbsp;ml filter tips that were modified to widen the tip opening using a sterilized razor blade. For the sterilized feces treatments (SC, SG), fecal pellets were sterilized in a pressure cooker for 40 minutes at 120 °C and then applied in the same manner as fresh feces. The fresh feces used in the experiment were subsampled for DNA extractions by preservation in DNA/RNA Shield (Zymo Research, CA). No manipulation was conducted on the no-feces control fragments. The experiment ran for ~22 hours; this treatment duration is reflective of time periods over which corals may sometimes be in direct contact with fish feces&nbsp;in situ&nbsp;(up to 48 hours;&nbsp;Ezzat et&nbsp;al., 2019;&nbsp;Ezzat et&nbsp;al., 2021).&nbsp;</p>
<p>Minor modifications were incorporated into the design of the second and third experimental iterations: for these iterations, five replicate colonies were used instead of four. Additionally, fecal treatments in these iterations were composed of feces from two individuals per fish species that were mixed prior to application (instead of using feces from a single fish individual per treatment as in the first experimental iteration).</p>
<p>To test how microbial communities in fish feces affected coral health, we quantified the frequencies and sizes of coral lesions caused by fecal treatments (in addition to measuring the photosynthetic efficiency of each fragment). In brief, fecal pellets were removed from each fragment and photographs were taken using a dissection microscope.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Dissection microscope photographs were analyzed in ImageJ (version&nbsp;1.53f51; Abràmoff, 2004). Each fragment was binned to one of three categories: “apparently healthy” (no change in fragment compared to before fecal application), “lesion” (fragment contains a novel patch of bare calcium carbonate, where coral tissue died and sloughed off following fecal application), or “dead” (fragment no longer contains live tissue; all tissue sloughed off following fecal application). The size of each lesion (excluding fragments binned as “apparently healthy” or “dead”) was measured by determining the average polyp size on each fragment in ImageJ by counting the number of polyps in a polygon of standardized size in triplicate. Then, the size of the lesion was measured (in pixels), and the lesion size was expressed as the estimated number of polyps that had died.</p>
Specified by the Principal Investigator(s)
- Imported original files "Lesion frequencies.CSV" and "Lesion sizes.CSV" into the BCO-DMO system.
- Concatenated "graco_iteration", "Treatment", and "Colony" columns into a sample id for each table to join on
- Joined tables into single dataset "933832_v1_coral_lesion_fish_feces.csv"
- Renamed fields to comply with system requirements
- Added month of collection column as indicated in methods section
- Added columns for lat and lon of reef as a general collection location
- Saved final file as "933832_v1_coral_lesion_fish_feces.csv"
- Checked all scientific names referenced in methods section and the methods section of related dataset using World Register of Marine Species (WoRMS) Taxon Match. All scientific names referenced are valid and accepted names as of 2024-09-23. Included the complete list in taxon_identifiers.csv as a supplemental file
- Included "Identification_in_methods", "matched_AphiaID', "LSID", "AphiaID_accepted", and "ScientificName_accepted" in "taxon_identifiers.csv" supplemental file
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Imaging PAM maxi (k-6) (Walz, Germany)
Imaging PAM maxi (k-6) (Walz, Germany)
PI Supplied Instrument Name: Imaging PAM maxi (k-6) (Walz, Germany) Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
Dissection scope (AmScope SM-1TSZZ-144S-10M)
Dissection scope (AmScope SM-1TSZZ-144S-10M)
PI Supplied Instrument Name: Dissection scope (AmScope SM-1TSZZ-144S-10M) PI Supplied Instrument Description:To test how microbial communities in fish feces affected coral health, we quantified the frequencies and sizes of coral lesions caused by fecal treatments (in addition to measuring the photosynthetic efficiency of each fragment). In brief, fecal pellets were removed from each fragment and photographs were taken using a dissection microscope. Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/