http://lod.bco-dmo.org/id/dataset/935794
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-08-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Hydrologic data from field sampling sites in the Neuse River Estuary, Pamlico Sound, and Onslow Bay in the coastal North Atlantic offshore from North Carolina (USA) in 2021 and 2022
2024-08-19
publication
2024-08-19
revision
BCO-DMO Linked Data URI
2024-08-19
creation
http://lod.bco-dmo.org/id/dataset/935794
Ryan Paerl
North Carolina State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Paerl, R., Curtis, N. (2024) Hydrologic data from field sampling sites in the Neuse River Estuary, Pamlico Sound, and Onslow Bay in the coastal North Atlantic offshore from North Carolina (USA) in 2021 and 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-08-19 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/935794 [access date]
Methods and Sampling: <p>Sampling summary:<br />
Discrete samples were all obtained from ~0.2m using a diaphragm pump and weighted, marked hose. All containers were kept in dark coolers at ambient temperature during transport to the laboratory.&nbsp; All filtration was done within a few hours of collection and when conditions permitted, on board the research vessel.<br />
<br />
Water sampling was conducted bi-weekly. When the collection was split over two days, a single date was used based on the upstream or majority stations.</p>
<hr />
<p>Methodology summaries corresponding to data columns in the dataset:</p>
<p>In-situ YSI sampling (YSI_* columns):</p>
<p>In situ measurements were performed at discrete depths on the sunlit side of the research vessel using a Yellow Springs Instruments (YSI Incoporated, Ohio) multiparameter sonde (Model 6600 or 6600 EDS-S Extended Deployment System) equipped with a YSI conductivity/temperature probe (Model 6560), a YSI chlorophyll probe (Model 6025), a YSI pH probe (Model 6561 or 6566), a YSI pulsed dissolved oxygen probe (Model 6562), a self cleaning YSI turbidity probe (Model 6026 or 6136), and beginning on the 07/30/2003 sampling date, a flat Li-Cor sensor (UWQ-PAR 6067).&nbsp; The YSI sonde was coupled to a either a YSI 610 DM datalogger or a YSI 650 MDS Multi-parameter Display System datalogger. The data were stored on the datalogger and downloaded to EcoWatch&nbsp;(v1.0.5.15, YSI software)&nbsp;upon return to the laboratory.</p>
<p>"PAR" column:<br />
<br />
The diffuse light attenuation coefficient, Kd, was calculated from depth profiles of photosynthetically active radiation (PAR, 400-700 nm).&nbsp; Prior to the 07/30/2003 sampling date, PAR measurements were performed with a spherical underwater quantum sensor (LI-COR LI-193SA) coupled to a LI-COR LI-1000 datalogger.&nbsp; Beginning on the 07/30/2003 sampling date, a flat underwater quantum sensor (LI-COR LI-193SA) attached to a Yellow Springs Instruments YSI 6600 or YSI 6600 EDS-S sonde was used to measure PAR.&nbsp; Measurements of PAR were performed on the sunlit side of the research vessel in 0.5 meter depth increments, beginning just below the water surface.&nbsp; The diffuse attenuation coefficient is the slope of the linear regression between natural log transformed PAR data and depth.&nbsp;</p>
<p>"POC" and "PN" columns:<br />
<br />
Particulate organic carbon (POC) and Particulate Nitrogen (PN) concentrations were determined by elemental analysis of material collected on pre-combusted Whatman GF/F glass fiber filters.&nbsp; Carbonates were removed from the filters by vapor phase acidification using concentrated hydrochloric acid (HCl).&nbsp; After drying at 60 0C, the filters were rolled in tin disks and injected into a PE 2400 Series II CHNS/O Analyzer calibrated with acetanilide ending in June 2014.&nbsp; Starting on the Neuse River sample date of June 2, 2014, a Costech Analytical Technologies, Inc. Elemental Combustion System CHNS-O ECS 4010 was used for elemental analysis by "flash combustion/chromatographic separation and multi-detector techniques".&nbsp; The Costech Instrument utilizes EAS Clarity Software.&nbsp; Atropine standards are used to develop a calibration curve (C 70.56%, N 4.84%, and carbon response ratio of 0.025 +/-0.003).&nbsp; NIST Buffalo River Sediment Reference Material 8704 (C 3.351% +/-0.017, N 0.20% +/-0.04) and/or Acetanilide Bypass (C 71.09%, N 10.36%, carbon response ratio of 0.055 +/- 0.003) may used for calibration or a check standard.<br />
<br />
"DOC" column:</p>
<p>Dissolved organic carbon (DOC) concentration was measured using a Shimadzu TOC-5000A Analyzer:&nbsp; Water samples were vacuum filtered (less than 25 kilopascal) using pre-combusted Whatman glass microfibre filters (GF/F).&nbsp; The filtrate was stored in pre-combusted glass scintillation vials with Teflon closures and frozen at -20 degrees Celsius until analysis.&nbsp; The Shimadzu TOC-5000A Analyzer uses high temperature catalytic oxidation followed by non-dispersive infrared analysis of the CO2 produced.&nbsp; Samples were acidified to a pH less than 2 and sparged with air before they were analyzed for non-volatile organic carbon.&nbsp;&nbsp;<br />
<br />
"DIC" column:</p>
<p>Dissolved inorganic carbon (DIC) was measured on samples held overnight in research pond by acidification followed by infrared analysis of carbon dioxide (CO2) on a Shimadzu Total Organic Carbon Analyzer (TOC-5000A) in IC mode and calibrated with sodium carbonate standards.&nbsp; Previously run ModMon samples resulted in standard error analysis measurement (RSD) of DIC +/- 0.94%.&nbsp;&nbsp;<br />
<br />
"NO3_NO2" column:</p>
<p>Nitrate/nitrite (NO3- / NO2-) concentration was determined using a Lachat/Zellweger Analytics QuikChem 8000 flow injection autoanalyzer (Milwaukee, WI, USA) using method FIA 31-107-04-1-C:&nbsp; Water samples were vacuum filtered (less than 25 kiloPascals) using pre-combusted Whatman glass microfibre filters (GF/F).&nbsp; The filtrate was stored in high-density polyethylene bottles and frozen at -20 degrees Celsius until analysis.&nbsp; Two replicates were run from the same bottle.&nbsp; Method detection limits (MDL, µg L-1) were 0.71 on 1/25/2020.<br />
<br />
"NH4" column:</p>
<p>Ammonium (NH4+) concentration was determined using a Lachat/Zellweger Analytics QuikChem 8000 flow injection autoanalyzer (Milwaukee, WI) using method FIA 31-107-06-1-A/B:&nbsp; Water samples were vacuum filtered (less than 25 kiloPascals) using pre-combusted Whatman glass microfibre filters (GF/F).&nbsp; The filtrate was stored in high-density polyethylene bottles and frozen (-20 degrees Celsius) until analysis.&nbsp; Two replicates were run from the same bottle.&nbsp; &nbsp;Method detection limits (MDL, µg L-1) were: 6.99 on 1/25/2020.</p>
<p>"TDN" column:<br />
<br />
Total dissolved nitrogen (TDN) was measured by in-line digestion using the Lachat/Zellweger Analytics QuikChem 8000 flow injection autoanalyzer (Milwaukee, WI, USA) using method FIA 31-107-04-3-B for low total nitrogen for brackish/fresh waters (detection level: 0.1 - 5.0 milligrams nitrogen per liter):&nbsp; Water samples were vacuum filtered (less than 25 kiloPascals) using pre-combusted Whatman glass microfibre filters (GF/F).&nbsp; The filtrate was stored in high-density polyethylene bottles and frozen at -20 degrees Celsius until analysis.&nbsp; Two replicates were run from the same bottle.&nbsp; Total dissolved nitrogen by in-line digestion works by oxidizing all the nitrogen compounds to nitrate by heating to 100 degrees Celsius and adding energy via UV light.&nbsp; The pH is dropped from 9.1 to 3 during the decomposition.&nbsp; The entire digestion occurs prior to the injection valve.&nbsp; The nitrate/nitrite concentration is then determined using standard colorimetric techniques similar to the strict nitrate/nitrite manifold. Method detection limits (MDL, µg L-1) were 11.75 on 1/25/2020.&nbsp;</p>
<p>"DON" column:<br />
<br />
Dissolved organic nitrogen (DON) was calculated by subtracting dissolved inorganic nitrogen (DIN) from total dissolved nitrogen (TDN).&nbsp; If the DIN value used in the calculation was below the detection limit, it was taken to be zero for this calculation.&nbsp; At one point DON was determined by high temperature oxidation using the Antek 7000N or Antek 7000V analyzer.<br />
<br />
"PO4" column:</p>
<p>Orthophosphate (PO43-) was determined using a Lachat/Zellweger Analytics QuikChem 8000 flow injection autoanalyzer (Milwaukee, WI) using method FIA 31-115-01-1-F/G:&nbsp; Water samples were vacuum filtered (less than 25 kiloPascals) using pre-combusted Whatman glass microfibre filters (GF/F).&nbsp; The filtrate was stored in high-density polyethylene bottles and frozen at -20 degrees Celsius until analysis.&nbsp; Two replicates were run from the same bottle.&nbsp; Method detection limits (MDL, µg L-1) were 6.56 on 1/25/2020.<br />
<br />
"SiO2" column:</p>
<p>Silicic acid (SiO2) was measured after vacuum filtration (&lt; 25 kPA) of the collected water samples through pre-combusted (3-4 hours at 450 0C) Whatman GF/F glass fiber filters.&nbsp; The filtrate was stored in high-density polyethylene bottles and frozen (-20 0C) until analysis.&nbsp; Two replicates were run from the same sample bottle.&nbsp; Nitrate plus nitrite concentrations were determined using a Lachat QuikChem 8000 flow injection autoanalyzer (Milwaukee, WI, USA).&nbsp; Method detection limits (MDL, µM) were 1.17 on 1/25/2020.<br />
<br />
"PPR" column:</p>
<p>Primary Productivity rate was measured using an adaptation of Steeman Nielsen's (1952) 14C bicarbonate method (Paerl et al. 1998).&nbsp; This method of measuring primary productivity allows direct measurement of carbon uptake and measures only net photosynthesis:&nbsp; Water samples were stored in 10 Liter high density polyethylene containers overnight in the research pond, a flow through system that receives water from the adjacent Bogue Sound, thereby simulating ambient water temperatures.&nbsp; The following morning the water samples were removed from the pond and transported to the laboratory for analysis.&nbsp; Water samples (76 milliliters) were added to three clear plastic square bottles to determine light uptake of carbon in triplicate and to 1 dark bottle to determine dark uptake of carbon.&nbsp; A solution of radioactive carbonate (300 microliters) was added to each bottle.&nbsp; The bottles were incubated for 4 hours in the pond.&nbsp; The light bottles were incubated underneath a field light simulator, while the dark bottles were incubated in a covered perforated bucket that was submerged in the pond.&nbsp; The FLS was used to simulate the ambient light conditions that phytoplankton are exposed to in the estuary (mixing conditions).&nbsp; The FLS is comprised of a rotating wheel with varying levels of screening.&nbsp; During the incubation period, photosynthetically active radiation (PAR) measurements were performed using a 2 pi Li-Cor LI-192SA spherical quantum sensor attached to a Li-Cor data logger.&nbsp; After the incubation period, the samples were returned to the laboratory, shaken and the entire contents were gently vacuum filtered (less than 25 kilopascals) using 25 mm Whatman glass microfibre filters (GF/F).&nbsp; The filters were placed in wooden drying trays and treated with concentrated hydrochloric acid fumes for 40 minutes to an hour to remove inorganic 14C.&nbsp; The filters were folded in half and placed in 7 milliliter plastic scintillation vials.&nbsp; Five milliliters of liquid scintillation cocktail (ecolume or cytoscint) was added to the vials.&nbsp; The vials were capped, shaken, stored in the dark for 3-24 hours and then assayed for radioactivity using a Beckman liquid scintillation counter.&nbsp; In addition to the samples, triplicate voucher samples were used to quantify the radioactivity of the 14C added.&nbsp; Voucher samples consisted of 100 microliter of 14C and 100 microliters of phenylethylamine.&nbsp; These vials also received 5 milliliters of liquid scintillation cocktail.&nbsp; A background vial and two 14C background standards were used.&nbsp; &nbsp;The quantity of carbon fixed is proportional to the fraction of radioactive carbon assimilated.&nbsp; (Paerl, H.W., J.L. Pinckney, J.M. Fear, and B.L. Peierls 1998. Ecosystem responses to internal and watershed organic matter loading: consequences for hypoxia in the eutrophying Neuse River Estuary, North Carolina, USA. Marine Ecology Progress Series 166: 17-25; Steemann Nielsen, E. 1952. The use of radio-active carbon (C14) for measuring organic production in the sea. Journal du Conseil permanent international pour L'Exploration de la Mer 18: 117-140).<br />
<br />
"Correct_Chla_IV" column:</p>
<p>Chl a concentration was measured using the modified in vitro fluorescence technique in EPA Method 445.0 (Welshmeyer 1994, Arar et al.&nbsp; 1997): Each water sample was vacuum filtered (less than 25 kilopascals) in duplicate at low ambient light conditions using 25 mm Whatman glass microfibre filters (GF/F).&nbsp; The filters were blotted dry, wrapped in foil and frozen immediately at -20 degrees Celsius until analysis.&nbsp; Chlorophyll a was extracted from the filter using a tissue grinder and 10 mL of 90 percent reagent grade aqueous acetone (v/v with deionized water, Fisher Scientific NF/FCC Grade). The samples remained in the acetone overnight at -20 degrees Celsius.&nbsp; The extracts were filter-clarified using a centrifuge and analyzed on a Turner Designs Trilogy fluorometer.&nbsp; The value reported is the average chlorophyll a concentration measured from the two filters. MDL 0.025 ug/l.&nbsp; 7200-046 Chl a extraction non acid module, 436 EX/685 EM (460 LED). The fluorometer was calibrated with a known concentration of pure Chl a that was determined using a Shimadzu UV-160U spectrophotometer and the extinction coefficients of Jeffrey and Humphrey (1975).&nbsp; The calibration was checked daily against a solid secondary standard (Turner Designs, proprietary formula).&nbsp; &nbsp;</p>
<p>Diagnostic phytoplankton photopigments were identified, separated and quantified by high performance liquid chromatography (HLPC) coupled to an in-line photodiode array spectrophotometer (Jeffrey et al.&nbsp; 1997). See "Related Datasets" section for the HLPC data and methods).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2049388 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2049388
onGoing
Ryan Paerl
North Carolina State University
919.515.7276
135 Jordan Hall 2800 Faucette Drive
Raleigh
NC
27695
USA
rpaerl@ncsu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Station
Trip_Code
Site
Lat
Lon
Date
YSI_Time
ISO_DateTime_UTC
YSI_Depth
YSI_Temp
YSI_SpecCond
YSI_Salinity
YSI_Dosat
YSI_DO
YSI_pH
YSI_Turbidity
Correct_Chla_IV
YSI_BP
PAR_Depth
PAR
POC
PN
C_to_N
DOC
DOC_flag
DIC
NO3_NO2
NO3_NO2_flag
NH4
NH4_flag
TDN
DON
PO4
PO4_flag
SiO2
PPR
PPR_flag
Costech Analytical Technologies, Inc. Elemental Combustion System CHNS-O ECS 4010
PE 2400 Series II CHNS/O Analyzer
Lachat/Zellweger Analytics QuikChem 8000 flow injection autoanalyzer (Milwaukee, WI, USA)
YSI multiparameter sonde (Model 6600 or 6600 EDS-S Extended Deployment System) ...
Antek 7000N or Antek 7000V analyzer
Shimadzu UV-160U spectrophotometer
Shimadzu TOC-5000A Analyzer
theme
None, User defined
station
deployment number
site
latitude
longitude
date_local
time_local
ISO_DateTime_UTC
depth
water temperature at measurement depth
conductivity
salinity
O2 saturation
dissolved Oxygen
pH
turbidity
chlorophyll-a; fluorometric method
barometric pressure
PAR
particulate organic Carbon (POC)
Total Particulate Nitrogen
Carbon to Nitrogen ratio (C:N)
dissolved organic Carbon
No BCO-DMO term
dissolved inorganic Carbon
nitrate plus nitrite
flag
Ammonium
Total Dissolved Nitrogen
Dissolved Organic Nitrogen
reactive phosphorus (PO4)
silicate, silicon dioxide SiO2
primary production
featureType
BCO-DMO Standard Parameters
Costech International Elemental Combustion System (ECS) 4010
Elemental Analyzer
Flow Injection Analyzer
LI-COR Biospherical PAR Sensor
Multi Parameter Portable Meter
Particulate Organic Carbon/Nitrogen Analyzer
Spectrophotometer
Total Organic Carbon Analyzer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Vitamin B1 Limitation and Advantageous Use of B1-related Compounds by Marine Bacterioplankton
https://osprey.bco-dmo.org/project/834222
Collaborative Research: Vitamin B1 Limitation and Advantageous Use of B1-related Compounds by Marine Bacterioplankton
<p><strong>NSF Award Abstract:</strong><br />
Planktonic marine bacteria significantly impact global elemental cycling, productivity, and water quality. Recent evidence shows that abundant and diverse marine bacterioplankton require external vitamin B1 or precursors (B1 herein) to survive, and addition of these nutrients stimulates bacterial production. This suggests that it is favorable for most marine bacteria to rely on supplied B1, making B1 dynamics an environmentally relevant test case to study nutrient exchanges within the planktonic microbiome. Notably though, links between extracellular B1 availability and the composition, function, and fitness of marine bacteria are poorly understood. This project sheds light on 1) which activities and interactions are modulated by B1 availability, and 2) the benefits of exogenous B1 use by ubiquitous marine bacterioplankton. Experiments are being conducted to address uncertainty regarding B1 limitation of natural bacterioplankton, help predict plankton responses to natural or anthropogenic increases in B1 and reveal more on the rules governing nutrient exchange between plankton. Greater knowledge of the advantages of bacterial B1 use will benefit fields beyond oceanography, such as synthetic biology which focuses on streamlined microbial product generation. B1-deficiency in animals is a current concern in marine ecosystems. Greater knowledge of costs and quotas at the microbial level will best position the larger community to ask deficiency questions at other trophic levels. The teaching and training components of this project include support for graduate students and a post-doctoral scholar. The major outreach component is a newly conceived Mobile Aquatic Microbial Laboratory (MAML) that seeks to improve public awareness of aquatic microbes and their ecosystem impact, as well as convey concepts of nutrient limitation and why cells need vitamins. Pre- and post-assessment and social distancing measures are integrated into MAML, as are incentives for participants to share via social media images and contribute to the program.</p>
<p>This project investigates the impact of vitamin B1 and B1 congeners on bacterioplankton in marine ecosystems where vitamins have garnered little recent consideration as influential nutrients. The work is conducted in the Neuse River estuary, the second largest estuary in the lower USA, and in the offshore coastal ocean. Nutrient amendment experiments are being conducted to test whether B1/congeners modulate bacterioplankton growth, composition, and gene transcription (reflecting putative function). Complementary lab-based experiments with wildtype and genetically engineered bacterioplankton are being conducted to test whether use of exogenous B1/congener significantly improves cell fitness and to address why reliance upon exogenous B1/congener is so prevalent among wild bacterioplankton. Additional lab experiments with isolates are being conducted to examine the protein-related cost of B1 <em>de novo</em> biosynthesis versus exogenous B1/congener use as well as cell quotas of B1 and precursors using mass spectrometry techniques. The work reaches beyond recent genome-based extrapolations to address how exogenous B1/congener availability shapes bacterioplankton metabolism, community structure, microbial interactions, and fitness. </p>
VBLAM
largerWorkCitation
project
eng; USA
oceans
-76.6771
-76.2006
34.609368
35.1225
2021-09-27
2022-08-29
Pamlico Albemarle Sound System, NC and Coastal N. Atlantic Ocean.
0
BCO-DMO catalogue of parameters from Hydrologic data from field sampling sites in the Neuse River Estuary, Pamlico Sound, and Onslow Bay in the coastal North Atlantic offshore from North Carolina (USA) in 2021 and 2022
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/966707.rdf
Name: Station
Units: unitless
Description: <p>Sampled station</p>
http://lod.bco-dmo.org/id/dataset-parameter/966708.rdf
Name: Trip_Code
Units: unitless
Description: <p>Individual trip code</p>
http://lod.bco-dmo.org/id/dataset-parameter/966709.rdf
Name: Site
Units: unitless
Description: <p>Site name (geolocation)</p>
http://lod.bco-dmo.org/id/dataset-parameter/966710.rdf
Name: Lat
Units: decimal degrees
Description: <p>Site latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/966711.rdf
Name: Lon
Units: decimal degrees
Description: <p>Site longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/966712.rdf
Name: Date
Units: unitless
Description: <p>Date when the in situ measurements were made. When the collection was split over two days, a single date was used based on the upstream or majority stations. (local time zone EST/EDT)</p>
http://lod.bco-dmo.org/id/dataset-parameter/966713.rdf
Name: YSI_Time
Units: unitless
Description: <p>Time when the in situ measurements were made. This time is an approximate water sampling time. (local time zone EST/EDT).</p>
http://lod.bco-dmo.org/id/dataset-parameter/966714.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Timestamp when the in situ measurements were made. This time is an approximate water sampling time. (UTC time zone, ISO 8601 format).</p>
http://lod.bco-dmo.org/id/dataset-parameter/966715.rdf
Name: YSI_Depth
Units: meters (m)
Description: <p>Exact depth (meters) where the in situ measurements were made.</p>
http://lod.bco-dmo.org/id/dataset-parameter/966716.rdf
Name: YSI_Temp
Units: degrees Celsius
Description: <p>In situ water temperature.</p>
http://lod.bco-dmo.org/id/dataset-parameter/966717.rdf
Name: YSI_SpecCond
Units: milliSiemens per centimeter (mS/cm)
Description: <p>In situ specific conductivity.</p>
http://lod.bco-dmo.org/id/dataset-parameter/966718.rdf
Name: YSI_Salinity
Units: parts per thousand (ppt)
Description: <p>In situ salinity.</p>
http://lod.bco-dmo.org/id/dataset-parameter/966719.rdf
Name: YSI_Dosat
Units: percent (%)
Description: <p>In situ dissolved oxygen saturation.</p>
http://lod.bco-dmo.org/id/dataset-parameter/966720.rdf
Name: YSI_DO
Units: milligrams per liter (mg/L)
Description: <p>In situ dissolved oxygen concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/966721.rdf
Name: YSI_pH
Units: unitless
Description: <p>In situ pH.</p>
http://lod.bco-dmo.org/id/dataset-parameter/966722.rdf
Name: YSI_Turbidity
Units: Nephelometric Turbidity Unit (NTU)
Description: <p>In situ turbidity (NTU).</p>
http://lod.bco-dmo.org/id/dataset-parameter/966723.rdf
Name: Correct_Chla_IV
Units: micrograms per liter (ug/L)
Description: <p>Extracted chlorophyll a based on the EPA method (Welshmeyer 1994, Arar et al. 1997). Chlorophyll a concentration measured by in vitro fluorometry.</p>
http://lod.bco-dmo.org/id/dataset-parameter/966724.rdf
Name: YSI_BP
Units: millimeters of mercury (mm Hg)
Description: <p>Surface barometric pressure (millimeters of mercury).</p>
http://lod.bco-dmo.org/id/dataset-parameter/966725.rdf
Name: PAR_Depth
Units: meters (m)
Description: <p>Depth of PAR measurement based on YSI 6600 or 6600 EDS-S sonde.</p>
http://lod.bco-dmo.org/id/dataset-parameter/966726.rdf
Name: PAR
Units: micromoles per square meter per second (umol m-2 s-1)
Description: <p>Photosynthetically Active Radiation (PAR).</p>
http://lod.bco-dmo.org/id/dataset-parameter/966727.rdf
Name: POC
Units: micrograms per liter (ug/L)
Description: <p>Particulate organic carbon concentration (micrograms of carbon per liter).</p>
http://lod.bco-dmo.org/id/dataset-parameter/966728.rdf
Name: PN
Units: micrograms per liter (ug/L)
Description: <p>Particulate nitrogen concentration (micrograms of nitrogen per liter).</p>
http://lod.bco-dmo.org/id/dataset-parameter/966729.rdf
Name: C_to_N
Units: unitless
Description: <p>Calculated molar ratio of particulate organic carbon (POC) to particulate nitrogen (PN).</p>
http://lod.bco-dmo.org/id/dataset-parameter/966730.rdf
Name: DOC
Units: micromolar (uM)
Description: <p>Dissolved organic carbon concentration (micromolar).</p>
http://lod.bco-dmo.org/id/dataset-parameter/966731.rdf
Name: DOC_flag
Units: unitless
Description: <p>DOC flag. Indicates if the blank value in the DOC column is due to the following:. BDL = ("Below the detection limit", NA= "Parameter was not analyzed/measured/recorded"). Flag "SUB" indicates the data in the DOC column is from NRE160 (adjacent station) used, NRE180 measurements not taken. SUB only occurs for the DOC column.</p>
http://lod.bco-dmo.org/id/dataset-parameter/966732.rdf
Name: DIC
Units: milligrams per liter (mg/L)
Description: <p>Dissolved inorganic carbon concentration (milligrams of carbon per liter).</p>
http://lod.bco-dmo.org/id/dataset-parameter/966733.rdf
Name: NO3_NO2
Units: micrograms per liter (ug/L)
Description: <p>Nitrate plus nitrite concentration (micrograms of nitrogen per liter).</p>
http://lod.bco-dmo.org/id/dataset-parameter/966734.rdf
Name: NO3_NO2_flag
Units: unitless
Description: <p>NO3_NO2 flag. Indicates if the blank value in the NO3_NO2 column is due to the following: BDL = "Below the detection limit", NA= "Parameter was not analyzed/measured/recorded"</p>
http://lod.bco-dmo.org/id/dataset-parameter/966735.rdf
Name: NH4
Units: micrograms per liter (ug/L)
Description: <p>Ammonium concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/966736.rdf
Name: NH4_flag
Units: unitless
Description: <p>NH4 flag. Indicates if the blank value in the NH4 column is due to the following: BDL = "Below the detection limit", NA= "Parameter was not analyzed/measured/recorded"</p>
<p> </p>
http://lod.bco-dmo.org/id/dataset-parameter/966737.rdf
Name: TDN
Units: micrograms per liter (ug/L)
Description: <p>Total dissolved nitrogen concentration (organic plus inorganic species in micrograms of nitrogen per liter).</p>
http://lod.bco-dmo.org/id/dataset-parameter/966738.rdf
Name: DON
Units: micrograms per liter (ug/L)
Description: <p>Calculated dissolved organic nitrogen concentration (micrograms of nitrogen per liter).</p>
http://lod.bco-dmo.org/id/dataset-parameter/966739.rdf
Name: PO4
Units: micrograms per liter (ug/L)
Description: <p>Orthophosphate concentration (micrograms of phosphorus per liter).</p>
http://lod.bco-dmo.org/id/dataset-parameter/966740.rdf
Name: PO4_flag
Units: unitless
Description: <p>PO4 flag. Indicates if the blank value in the PO4 column is due to the following: BDL = "Below the detection limit", NA= "Parameter was not analyzed/measured/recorded"</p>
http://lod.bco-dmo.org/id/dataset-parameter/966741.rdf
Name: SiO2
Units: micromolar (uM)
Description: <p>Silica concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/966742.rdf
Name: PPR
Units: milligrams per cubic meter per hour (mg C m-3 hr-1)
Description: <p>Primary productivity by light/dark 14C bicarbonate incorporation (milligrams of C per meter cubed per hour).</p>
http://lod.bco-dmo.org/id/dataset-parameter/966743.rdf
Name: PPR_flag
Units: unitless
Description: <p>PPR flag. Indicates if the blank value in the PPR column is due to the following: BDL = "Below the detection limit", NA= "Parameter was not analyzed/measured/recorded"</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/935794/data/download
download
onLine
dataset
<p>Sampling summary:<br />
Discrete samples were all obtained from ~0.2m using a diaphragm pump and weighted, marked hose. All containers were kept in dark coolers at ambient temperature during transport to the laboratory.&nbsp; All filtration was done within a few hours of collection and when conditions permitted, on board the research vessel.<br />
<br />
Water sampling was conducted bi-weekly. When the collection was split over two days, a single date was used based on the upstream or majority stations.</p>
<hr />
<p>Methodology summaries corresponding to data columns in the dataset:</p>
<p>In-situ YSI sampling (YSI_* columns):</p>
<p>In situ measurements were performed at discrete depths on the sunlit side of the research vessel using a Yellow Springs Instruments (YSI Incoporated, Ohio) multiparameter sonde (Model 6600 or 6600 EDS-S Extended Deployment System) equipped with a YSI conductivity/temperature probe (Model 6560), a YSI chlorophyll probe (Model 6025), a YSI pH probe (Model 6561 or 6566), a YSI pulsed dissolved oxygen probe (Model 6562), a self cleaning YSI turbidity probe (Model 6026 or 6136), and beginning on the 07/30/2003 sampling date, a flat Li-Cor sensor (UWQ-PAR 6067).&nbsp; The YSI sonde was coupled to a either a YSI 610 DM datalogger or a YSI 650 MDS Multi-parameter Display System datalogger. The data were stored on the datalogger and downloaded to EcoWatch&nbsp;(v1.0.5.15, YSI software)&nbsp;upon return to the laboratory.</p>
<p>"PAR" column:<br />
<br />
The diffuse light attenuation coefficient, Kd, was calculated from depth profiles of photosynthetically active radiation (PAR, 400-700 nm).&nbsp; Prior to the 07/30/2003 sampling date, PAR measurements were performed with a spherical underwater quantum sensor (LI-COR LI-193SA) coupled to a LI-COR LI-1000 datalogger.&nbsp; Beginning on the 07/30/2003 sampling date, a flat underwater quantum sensor (LI-COR LI-193SA) attached to a Yellow Springs Instruments YSI 6600 or YSI 6600 EDS-S sonde was used to measure PAR.&nbsp; Measurements of PAR were performed on the sunlit side of the research vessel in 0.5 meter depth increments, beginning just below the water surface.&nbsp; The diffuse attenuation coefficient is the slope of the linear regression between natural log transformed PAR data and depth.&nbsp;</p>
<p>"POC" and "PN" columns:<br />
<br />
Particulate organic carbon (POC) and Particulate Nitrogen (PN) concentrations were determined by elemental analysis of material collected on pre-combusted Whatman GF/F glass fiber filters.&nbsp; Carbonates were removed from the filters by vapor phase acidification using concentrated hydrochloric acid (HCl).&nbsp; After drying at 60 0C, the filters were rolled in tin disks and injected into a PE 2400 Series II CHNS/O Analyzer calibrated with acetanilide ending in June 2014.&nbsp; Starting on the Neuse River sample date of June 2, 2014, a Costech Analytical Technologies, Inc. Elemental Combustion System CHNS-O ECS 4010 was used for elemental analysis by "flash combustion/chromatographic separation and multi-detector techniques".&nbsp; The Costech Instrument utilizes EAS Clarity Software.&nbsp; Atropine standards are used to develop a calibration curve (C 70.56%, N 4.84%, and carbon response ratio of 0.025 +/-0.003).&nbsp; NIST Buffalo River Sediment Reference Material 8704 (C 3.351% +/-0.017, N 0.20% +/-0.04) and/or Acetanilide Bypass (C 71.09%, N 10.36%, carbon response ratio of 0.055 +/- 0.003) may used for calibration or a check standard.<br />
<br />
"DOC" column:</p>
<p>Dissolved organic carbon (DOC) concentration was measured using a Shimadzu TOC-5000A Analyzer:&nbsp; Water samples were vacuum filtered (less than 25 kilopascal) using pre-combusted Whatman glass microfibre filters (GF/F).&nbsp; The filtrate was stored in pre-combusted glass scintillation vials with Teflon closures and frozen at -20 degrees Celsius until analysis.&nbsp; The Shimadzu TOC-5000A Analyzer uses high temperature catalytic oxidation followed by non-dispersive infrared analysis of the CO2 produced.&nbsp; Samples were acidified to a pH less than 2 and sparged with air before they were analyzed for non-volatile organic carbon.&nbsp;&nbsp;<br />
<br />
"DIC" column:</p>
<p>Dissolved inorganic carbon (DIC) was measured on samples held overnight in research pond by acidification followed by infrared analysis of carbon dioxide (CO2) on a Shimadzu Total Organic Carbon Analyzer (TOC-5000A) in IC mode and calibrated with sodium carbonate standards.&nbsp; Previously run ModMon samples resulted in standard error analysis measurement (RSD) of DIC +/- 0.94%.&nbsp;&nbsp;<br />
<br />
"NO3_NO2" column:</p>
<p>Nitrate/nitrite (NO3- / NO2-) concentration was determined using a Lachat/Zellweger Analytics QuikChem 8000 flow injection autoanalyzer (Milwaukee, WI, USA) using method FIA 31-107-04-1-C:&nbsp; Water samples were vacuum filtered (less than 25 kiloPascals) using pre-combusted Whatman glass microfibre filters (GF/F).&nbsp; The filtrate was stored in high-density polyethylene bottles and frozen at -20 degrees Celsius until analysis.&nbsp; Two replicates were run from the same bottle.&nbsp; Method detection limits (MDL, µg L-1) were 0.71 on 1/25/2020.<br />
<br />
"NH4" column:</p>
<p>Ammonium (NH4+) concentration was determined using a Lachat/Zellweger Analytics QuikChem 8000 flow injection autoanalyzer (Milwaukee, WI) using method FIA 31-107-06-1-A/B:&nbsp; Water samples were vacuum filtered (less than 25 kiloPascals) using pre-combusted Whatman glass microfibre filters (GF/F).&nbsp; The filtrate was stored in high-density polyethylene bottles and frozen (-20 degrees Celsius) until analysis.&nbsp; Two replicates were run from the same bottle.&nbsp; &nbsp;Method detection limits (MDL, µg L-1) were: 6.99 on 1/25/2020.</p>
<p>"TDN" column:<br />
<br />
Total dissolved nitrogen (TDN) was measured by in-line digestion using the Lachat/Zellweger Analytics QuikChem 8000 flow injection autoanalyzer (Milwaukee, WI, USA) using method FIA 31-107-04-3-B for low total nitrogen for brackish/fresh waters (detection level: 0.1 - 5.0 milligrams nitrogen per liter):&nbsp; Water samples were vacuum filtered (less than 25 kiloPascals) using pre-combusted Whatman glass microfibre filters (GF/F).&nbsp; The filtrate was stored in high-density polyethylene bottles and frozen at -20 degrees Celsius until analysis.&nbsp; Two replicates were run from the same bottle.&nbsp; Total dissolved nitrogen by in-line digestion works by oxidizing all the nitrogen compounds to nitrate by heating to 100 degrees Celsius and adding energy via UV light.&nbsp; The pH is dropped from 9.1 to 3 during the decomposition.&nbsp; The entire digestion occurs prior to the injection valve.&nbsp; The nitrate/nitrite concentration is then determined using standard colorimetric techniques similar to the strict nitrate/nitrite manifold. Method detection limits (MDL, µg L-1) were 11.75 on 1/25/2020.&nbsp;</p>
<p>"DON" column:<br />
<br />
Dissolved organic nitrogen (DON) was calculated by subtracting dissolved inorganic nitrogen (DIN) from total dissolved nitrogen (TDN).&nbsp; If the DIN value used in the calculation was below the detection limit, it was taken to be zero for this calculation.&nbsp; At one point DON was determined by high temperature oxidation using the Antek 7000N or Antek 7000V analyzer.<br />
<br />
"PO4" column:</p>
<p>Orthophosphate (PO43-) was determined using a Lachat/Zellweger Analytics QuikChem 8000 flow injection autoanalyzer (Milwaukee, WI) using method FIA 31-115-01-1-F/G:&nbsp; Water samples were vacuum filtered (less than 25 kiloPascals) using pre-combusted Whatman glass microfibre filters (GF/F).&nbsp; The filtrate was stored in high-density polyethylene bottles and frozen at -20 degrees Celsius until analysis.&nbsp; Two replicates were run from the same bottle.&nbsp; Method detection limits (MDL, µg L-1) were 6.56 on 1/25/2020.<br />
<br />
"SiO2" column:</p>
<p>Silicic acid (SiO2) was measured after vacuum filtration (&lt; 25 kPA) of the collected water samples through pre-combusted (3-4 hours at 450 0C) Whatman GF/F glass fiber filters.&nbsp; The filtrate was stored in high-density polyethylene bottles and frozen (-20 0C) until analysis.&nbsp; Two replicates were run from the same sample bottle.&nbsp; Nitrate plus nitrite concentrations were determined using a Lachat QuikChem 8000 flow injection autoanalyzer (Milwaukee, WI, USA).&nbsp; Method detection limits (MDL, µM) were 1.17 on 1/25/2020.<br />
<br />
"PPR" column:</p>
<p>Primary Productivity rate was measured using an adaptation of Steeman Nielsen's (1952) 14C bicarbonate method (Paerl et al. 1998).&nbsp; This method of measuring primary productivity allows direct measurement of carbon uptake and measures only net photosynthesis:&nbsp; Water samples were stored in 10 Liter high density polyethylene containers overnight in the research pond, a flow through system that receives water from the adjacent Bogue Sound, thereby simulating ambient water temperatures.&nbsp; The following morning the water samples were removed from the pond and transported to the laboratory for analysis.&nbsp; Water samples (76 milliliters) were added to three clear plastic square bottles to determine light uptake of carbon in triplicate and to 1 dark bottle to determine dark uptake of carbon.&nbsp; A solution of radioactive carbonate (300 microliters) was added to each bottle.&nbsp; The bottles were incubated for 4 hours in the pond.&nbsp; The light bottles were incubated underneath a field light simulator, while the dark bottles were incubated in a covered perforated bucket that was submerged in the pond.&nbsp; The FLS was used to simulate the ambient light conditions that phytoplankton are exposed to in the estuary (mixing conditions).&nbsp; The FLS is comprised of a rotating wheel with varying levels of screening.&nbsp; During the incubation period, photosynthetically active radiation (PAR) measurements were performed using a 2 pi Li-Cor LI-192SA spherical quantum sensor attached to a Li-Cor data logger.&nbsp; After the incubation period, the samples were returned to the laboratory, shaken and the entire contents were gently vacuum filtered (less than 25 kilopascals) using 25 mm Whatman glass microfibre filters (GF/F).&nbsp; The filters were placed in wooden drying trays and treated with concentrated hydrochloric acid fumes for 40 minutes to an hour to remove inorganic 14C.&nbsp; The filters were folded in half and placed in 7 milliliter plastic scintillation vials.&nbsp; Five milliliters of liquid scintillation cocktail (ecolume or cytoscint) was added to the vials.&nbsp; The vials were capped, shaken, stored in the dark for 3-24 hours and then assayed for radioactivity using a Beckman liquid scintillation counter.&nbsp; In addition to the samples, triplicate voucher samples were used to quantify the radioactivity of the 14C added.&nbsp; Voucher samples consisted of 100 microliter of 14C and 100 microliters of phenylethylamine.&nbsp; These vials also received 5 milliliters of liquid scintillation cocktail.&nbsp; A background vial and two 14C background standards were used.&nbsp; &nbsp;The quantity of carbon fixed is proportional to the fraction of radioactive carbon assimilated.&nbsp; (Paerl, H.W., J.L. Pinckney, J.M. Fear, and B.L. Peierls 1998. Ecosystem responses to internal and watershed organic matter loading: consequences for hypoxia in the eutrophying Neuse River Estuary, North Carolina, USA. Marine Ecology Progress Series 166: 17-25; Steemann Nielsen, E. 1952. The use of radio-active carbon (C14) for measuring organic production in the sea. Journal du Conseil permanent international pour L'Exploration de la Mer 18: 117-140).<br />
<br />
"Correct_Chla_IV" column:</p>
<p>Chl a concentration was measured using the modified in vitro fluorescence technique in EPA Method 445.0 (Welshmeyer 1994, Arar et al.&nbsp; 1997): Each water sample was vacuum filtered (less than 25 kilopascals) in duplicate at low ambient light conditions using 25 mm Whatman glass microfibre filters (GF/F).&nbsp; The filters were blotted dry, wrapped in foil and frozen immediately at -20 degrees Celsius until analysis.&nbsp; Chlorophyll a was extracted from the filter using a tissue grinder and 10 mL of 90 percent reagent grade aqueous acetone (v/v with deionized water, Fisher Scientific NF/FCC Grade). The samples remained in the acetone overnight at -20 degrees Celsius.&nbsp; The extracts were filter-clarified using a centrifuge and analyzed on a Turner Designs Trilogy fluorometer.&nbsp; The value reported is the average chlorophyll a concentration measured from the two filters. MDL 0.025 ug/l.&nbsp; 7200-046 Chl a extraction non acid module, 436 EX/685 EM (460 LED). The fluorometer was calibrated with a known concentration of pure Chl a that was determined using a Shimadzu UV-160U spectrophotometer and the extinction coefficients of Jeffrey and Humphrey (1975).&nbsp; The calibration was checked daily against a solid secondary standard (Turner Designs, proprietary formula).&nbsp; &nbsp;</p>
<p>Diagnostic phytoplankton photopigments were identified, separated and quantified by high performance liquid chromatography (HLPC) coupled to an in-line photodiode array spectrophotometer (Jeffrey et al.&nbsp; 1997). See "Related Datasets" section for the HLPC data and methods).</p>
Specified by the Principal Investigator(s)
<p>Formatting and organization of data was completed in Excel (Microsoft).</p>
Specified by the Principal Investigator(s)
BCO-DMO Data Manager Processing Notes:
* Data from source file BLAMM_METADATA_7_3_24_RWP.xlsx Sheet "Hydrologic_conditions" were exported from Excel as a csv file. The csv file was imported into the BCO-DMO data system.
* Additional *_flag columns added. For example, DOC_flag for the DOC column. _flag columns provide information about what type of missing data each blank value corresponds to or if substituted data was used. Flags (-7777,-9999, and asterisk characters) within data columns moved to the new _flag columns as (NA,BDL,and SUB), respectively. See the Parameters section for more details on flag meanings.
** Missing data in the BCO-DMO system displays as a blank (null values) by default and will vary depending upon the file format downloaded (blank in csv files, NaN in .mat matlab files, etc).
* Parameters (column names) renamed to comply with BCO-DMO naming conventions. See https://www.bco-dmo.org/page/bco-dmo-data-processing-conventions
* Site name (geolocation), lat, lon were added to the data table from an additional sheet provided in the excel file.
* DateTime (UTC) column added in ISO 8601 format using local date and time provided (assumed to be EST/EDT but confirming with the data provider). Date and time format changed to ISO 8601 format.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Costech Analytical Technologies, Inc. Elemental Combustion System CHNS-O ECS 4010
Costech Analytical Technologies, Inc. Elemental Combustion System CHNS-O ECS 4010
PI Supplied Instrument Name: Costech Analytical Technologies, Inc. Elemental Combustion System CHNS-O ECS 4010 Instrument Name: Costech International Elemental Combustion System (ECS) 4010 Instrument Short Name:Costech ECS 4010 Instrument Description: The ECS 4010 Nitrogen / Protein Analyzer is an elemental combustion analyser for CHNSO elemental analysis and Nitrogen / Protein determination. The GC oven and separation column have a temperature range of 30-110 degC, with control of +/- 0.1 degC.
PE 2400 Series II CHNS/O Analyzer
PE 2400 Series II CHNS/O Analyzer
PI Supplied Instrument Name: PE 2400 Series II CHNS/O Analyzer Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
Lachat/Zellweger Analytics QuikChem 8000 flow injection autoanalyzer (Milwaukee, WI, USA)
Lachat/Zellweger Analytics QuikChem 8000 flow injection autoanalyzer (Milwaukee, WI, USA)
PI Supplied Instrument Name: Lachat/Zellweger Analytics QuikChem 8000 flow injection autoanalyzer (Milwaukee, WI, USA) Instrument Name: Flow Injection Analyzer Instrument Short Name:FIA Instrument Description: An instrument that performs flow injection analysis. Flow injection analysis (FIA) is an approach to chemical analysis that is accomplished by injecting a plug of sample into a flowing carrier stream. FIA is an automated method in which a sample is injected into a continuous flow of a carrier solution that mixes with other continuously flowing solutions before reaching a detector. Precision is dramatically increased when FIA is used instead of manual injections and as a result very specific FIA systems have been developed for a wide array of analytical techniques. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB36/
PI Supplied Instrument Name: PI Supplied Instrument Description:spherical underwater quantum sensor (LI-COR LI-193SA) coupled to a LI-COR LI-1000 datalogger Instrument Name: LI-COR Biospherical PAR Sensor Instrument Short Name:LI-COR Biospherical PAR Instrument Description: The LI-COR Biospherical PAR Sensor is used to measure Photosynthetically Available Radiation (PAR) in the water column. This instrument designation is used when specific make and model are not known. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0074/
YSI multiparameter sonde (Model 6600 or 6600 EDS-S Extended Deployment System) ...
YSI multiparameter sonde (Model 6600 or 6600 EDS-S Extended Deployment System) ...
PI Supplied Instrument Name: YSI multiparameter sonde (Model 6600 or 6600 EDS-S Extended Deployment System) ... PI Supplied Instrument Description:In situ measurements were performed at discrete depths on the sunlit side of the research vessel using a Yellow Springs Instruments (YSI Incoporated, Ohio) multiparameter sonde (Model 6600 or 6600 EDS-S Extended Deployment System) equipped with a YSI conductivity/temperature probe (Model 6560), a YSI chlorophyll probe (Model 6025), a YSI pH probe (Model 6561 or 6566), a YSI pulsed dissolved oxygen probe (Model 6562), a self cleaning YSI turbidity probe (Model 6026 or 6136), and beginning on the 07/30/2003 sampling date, a flat Li-Cor sensor (UWQ-PAR 6067). The YSI sonde was coupled to a either a YSI 610 DM datalogger or a YSI 650 MDS Multi-parameter Display System datalogger. The data were stored on the datalogger and downloaded to Ecowin software upon return to the laboratory. Instrument Name: Multi Parameter Portable Meter Instrument Short Name: Instrument Description: An analytical instrument that can measure multiple parameters, such as pH, EC, TDS, DO and temperature with one device and is portable or hand-held.
Antek 7000N or Antek 7000V analyzer
Antek 7000N or Antek 7000V analyzer
PI Supplied Instrument Name: Antek 7000N or Antek 7000V analyzer Instrument Name: Particulate Organic Carbon/Nitrogen Analyzer Instrument Short Name:CHN Instrument Description: A unit that accurately determines the carbon and nitrogen concentrations of organic compounds typically by detecting and measuring their combustion products (CO2 and NO).
Shimadzu UV-160U spectrophotometer
Shimadzu UV-160U spectrophotometer
PI Supplied Instrument Name: Shimadzu UV-160U spectrophotometer Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Shimadzu TOC-5000A Analyzer
Shimadzu TOC-5000A Analyzer
PI Supplied Instrument Name: Shimadzu TOC-5000A Analyzer Instrument Name: Total Organic Carbon Analyzer Instrument Short Name:TOC analyzer Instrument Description: A unit that accurately determines the carbon concentrations of organic compounds typically by detecting and measuring its combustion product (CO2). See description document at: http://bcodata.whoi.edu/LaurentianGreatLakes_Chemistry/bs116.pdf Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/