http://lod.bco-dmo.org/id/dataset/936158 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2024-08-23 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Neocalanus body and lipid sac morphometrics from zooplankton samples collected during Seward Line cruises in the Northern Gulf of Alaska from 2018 to 2020 2024-08-23 publication 2024-08-23 revision BCO-DMO Linked Data URI 2024-08-23 creation http://lod.bco-dmo.org/id/dataset/936158 Russell R. Hopcroft University of Alaska Fairbanks principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Hopcroft, R. R. (2024) Neocalanus body and lipid sac morphometrics from zooplankton samples collected during Seward Line cruises in the Northern Gulf of Alaska from 2018 to 2020. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-08-23 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/936158 [access date] Methods and Sampling: &lt;p&gt;&lt;!--[if gte mso 9]&gt;&lt;xml&gt; &lt;o&gt; &lt;o&gt;&lt;/o:AllowPNG&gt; &lt;/o:OfficeDocumentSettings&gt; &lt;/xml&gt;&lt;![endif]--&gt;&lt;!--[if gte mso 9]&gt;&lt;xml&gt; &lt;w&gt; &lt;w&gt;Normal&lt;/w:View&gt; &lt;w&gt;0&lt;/w:Zoom&gt; &lt;w&gt;&lt;/w:TrackMoves&gt; &lt;w&gt;&lt;/w:TrackFormatting&gt; &lt;w&gt;&lt;/w:PunctuationKerning&gt; &lt;w&gt;&lt;/w:ValidateAgainstSchemas&gt; &lt;w&gt;false&lt;/w:SaveIfXMLInvalid&gt; &lt;w&gt;false&lt;/w:IgnoreMixedContent&gt; &lt;w&gt;false&lt;/w:AlwaysShowPlaceholderText&gt; &lt;w&gt;&lt;/w:DoNotPromoteQF&gt; &lt;w&gt;EN-US&lt;/w:LidThemeOther&gt; &lt;w&gt;X-NONE&lt;/w:LidThemeAsian&gt; &lt;w&gt;X-NONE&lt;/w:LidThemeComplexScript&gt; &lt;w&gt; &lt;w&gt;&lt;/w:BreakWrappedTables&gt; &lt;w&gt;&lt;/w:SnapToGridInCell&gt; &lt;w&gt;&lt;/w:WrapTextWithPunct&gt; &lt;w&gt;&lt;/w:UseAsianBreakRules&gt; 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&lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;/w:LatentStyles&gt; &lt;/xml&gt;&lt;![endif]--&gt;&lt;!--[if gte mso 10]&gt; &lt;style&gt; /* Style Definitions */ table.MsoNormalTable {mso-style-name:&quot;Table Normal&quot;; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:&quot;&quot;; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-pagination:widow-orphan; font-size:12.0pt; font-family:&quot;Calibri&quot;,sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:&quot;Times New Roman&quot;; mso-bidi-theme-font:minor-bidi;} &lt;/style&gt; &lt;![endif]--&gt;&lt;/p&gt; &lt;p&gt;Zooplankton samples were collected on NGA-LTER cruises along the Seward Line and within PWS during the summer, spring, and fall of 2018-20 aboard the R/V &lt;em&gt;Sikuliaq&lt;/em&gt;, R/V &lt;em&gt;Tiglax&lt;/em&gt; or the R/V &lt;em&gt;Woldstad&lt;/em&gt;. During spring and summer cruises copepods were collected using a Quad net, a modified CalVet net (0.25 m mouth diameter, 1 m cylinder and 1.5 m cone), of 150-µm mesh hauled vertically from 100 m during the day, then live sorted and imaged. During summer 2019, animals were also collected during the night in the drogue net (0-200 m) of a towed Midi Multinet (0.25 m&lt;sup&gt;2&lt;/sup&gt;) (Hydro-Bios, Germany), equipped with 500-µm mesh nets, and live sorted and imaged the following morning. The towed Multinet fished five discrete depth layers including: 0-20, 20-40, 40-60, 60-100, and 100-200 m. During summer 2020, we employed a vertically-hauled Midi Multinet of 150-µm mesh (fished during the day) for these same strata that were live-sorted and imaged immediately after collection to improve specimen quality. During summer and fall of all years at one or two deep-water Seward Line stations, and one or two PWS stations, we also fished the vertically-hauled Multinet at four additional depths: 200-300, 300-400, 400-600 and 600-1200 m (or 600-720 for PWS). A flowmeter installed in each net quantified the volume of water filtered. We targeted &lt;em&gt;Neocalanus flemingeri&lt;/em&gt;, &lt;em&gt;Neocalanus plumchrus &lt;/em&gt;and&lt;em&gt; Neocalanus cristatus &lt;/em&gt;for sorting, staging, and imaging from each of these strata.&lt;/p&gt; &lt;p&gt;Up to 60 individuals of each stage for each species were analyzed, if present. The &lt;em&gt;Neocalanus&lt;/em&gt; specimens were selected individually from homogenized temperature-regulated subsamples using a ladle or wide-bore plastic pipette. Only living, apparently undamaged individuals with intact lipid sacs were sorted and imaged using a Leica MZ16 microscope with either a Spot 4Mpx, Jenoptik 8Mpx, or Spot 12Mpx digital camera. Depending on stage and species, just prior to imaging three to fifteen animals were placed in a chilled embryo dish then water was reduced so animals shifted onto their sides (with gentle manipulation as needed). For every magnification setting, a calibration scale bar was added to the images. In cases where availability of animals was limited, animals with compromised lipid sacs were imaged to establish prosome length only. The calibrated images were later analyzed using Spot Software (V4.7 or 5.3) for determination of the prosome length (measured as maximum) and prosome width (measured from top of prosome to base of maxillipeds), the lipid sac length (measured from most anterior point to most posterior point), and lipid sac width (measured starting at the point closest to the base of the maxillipeds (ventral point) to most dorsal point). Animals that showed signs of compromised lipid sacs upon image inspection were only measured for prosome length. Adobe Photoshop (version CS6) was utilized to measure lipid sac area using the quick select tool to help demarcate the perimeter of the lipid sac. The number of pixels within the perimeter of the lipid sac was converted to area (mm&lt;sup&gt;2&lt;/sup&gt;) using the pixel-to-µm calibration associated with each image.&lt;/p&gt; &lt;p&gt;To calculate lipid sac volume, we first assumed the lipid sac to be a cylinder. We used the measured lipid sac length and the measured lipid sac area to find the average diameter of the cylinder (i.e. area divided by length) in order to account for the varying height and shape of the lipid sac. We then used this average diameter to estimate the volume of the lipid sac.&amp;nbsp; Body volume wa calcualted at a oblate spheroid from prosome length and prosome height.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Species List:&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Scientfic_Name, Lifesciences Identifier (LSID)&lt;/strong&gt;&lt;br /&gt; Neocalanus flemingeri, urn:lsid:marinespecies.org:taxname:353708&lt;br /&gt; Neocalanus plumchrus, urn:lsid:marinespecies.org:taxname:196772&lt;br /&gt; Neocalanus cristatus, urn:lsid:marinespecies.org:taxname:104470&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756859 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756859 onGoing Russell R. Hopcroft University of Alaska Fairbanks 907-474-7842 College of Fisheries and Ocean Sciences, Institute of Marine Sciences 120 O'Neill Fairbanks AK 99775-7220 USA rrhopcroft@alaska.edu pointOfContact asNeeded Dataset Version: 1 Unknown OrgSequence Year Season station DateTime_local ISO_DateTime_UTC Latitude Longitude DephMax DepthMin species LifeStage ProsomeLength ProsomeHeight LipidLength LipidHeight LipidArea AdjustedHeight LipidVolume BodyVolume Lipid_percent_BodyVolume Hydrobios Multinet-midi both 150um and 500um mesh Digital cameras theme None, User defined individual year sample description station Date and time ISO_DateTime_UTC latitude longitude depth_max depth_min species scientific name (binomial) length area volume lipids in biota featureType BCO-DMO Standard Parameters MultiNet Plankton Net Underwater Camera instrument BCO-DMO Standard Instruments SKQ201810S SKQ201915S SKQ202006S SKQ202010S SKQ202012S WSD201807 TXS19 TXF19 TXF18 service Deployment Activity Northern Gulf of Alaska place Locations otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: Molecular profiling of the ecophysiology of dormancy induction in calanid copepods of the Northern Gulf of Alaska LTER site https://osprey.bco-dmo.org/project/720280 Collaborative Research: Molecular profiling of the ecophysiology of dormancy induction in calanid copepods of the Northern Gulf of Alaska LTER site <p>NSF Award Abstract:<br /> The sub-arctic Pacific sustains major fisheries with nearly all commercially important species depending either directly or indirectly on lipid-rich copepods (Neocalanus flemingeri, Neocalanus plumchrus, Neocalanus cristatus and Calanus marshallae). In turn, these species depend on a short-lived spring algal bloom for growth and the accumulation of lipid stores in order to complete an annual life cycle that includes a period of dormancy. The intellectual thrust of this project measures how the timing and magnitude of algal blooms affect preparation for dormancy using a combination of field and experimental observations. The Northern Gulf of Alaska - with four calanid species that experience dormancy, steep environmental gradients, well-described phytoplankton bloom dynamics, and a concurrent NSF-LTER program - provides an unusual opportunity to identify the factors that affect dormancy preparation. Education and outreach plans are integrated with the research. Educational efforts focus on interdisciplinary opportunities for undergraduate, graduate and post-doctoral trainees. The project will generate content for existing graduate and undergraduate courses. U. of Alaska Fairbanks and U. Hawaii at Manoa are Alaska Native and Native Hawaiian Serving Institutions, and students from these groups will be recruited to participate in the project. Because fishing is a major industry in the Gulf of Alaska, outreach will communicate the role copepods play in marine ecosystems using the concept of a dynamic food web tied to production cycles.</p> <p>Diapause (dormancy) and the accompanying accumulation of lipids in copepods have been identified as key drivers in high latitude ecosystems that support economically important fisheries, including those of the Gulf of Alaska. While the disappearance of lipid-rich copepods has been linked to severe declines in fish stocks, little is known about the environmental conditions that are required for the successful completion of the copepod's life cycle. A physiological profiling approach that measures relative gene expression will be used to test two alternative hypotheses: the lipid accumulation window hypothesis, which holds that individuals enter diapause only after they have accumulated sufficient lipid stores, and the developmental program hypothesis, which holds that once the diapause program is activated, progression occurs independent of lipid accumulation. The specific objectives are: 1) determine the effect of food levels during N. flemingeri copepodite stages on progression towards diapause using multiple physiological and developmental markers; 2) characterize the seasonal changes in the physiological profile of N. flemingeri across environmental gradients and across years; 3) compare physiological profiles across co-occurring calanid species (N. flemingeri, Neocalanus plumchrus, Neocalanus cristatus and Calanus marshallae); and 4) estimate the reproductive potential of the overwintering populations of N. flemingeri. The broader scientific significance includes the acquisition of new genomic data and molecular resources that will be made publicly available through established data repositories, and the development of new tools for routinely obtaining physiological profiles of copepods.</p> <p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> <p> </p> <p><strong>NOTE:</strong> Petra Lenz is a former Principal Investigator (PI) and Andrew Christie is a former Co-Principal Investigator (Co-PI) on this project (award #1756767). Daniel Hartline is the PI listed for the award #1756767 and is now a former Co-PI on this project.</p> Diapause preparation largerWorkCitation project eng; USA oceans Northern Gulf of Alaska -149.5 -147.6 57.75 60.6 2018-04-20 2018-09-30 Northern Gulf of Alaska LTER 0 BCO-DMO catalogue of parameters from Neocalanus body and lipid sac morphometrics from zooplankton samples collected during Seward Line cruises in the Northern Gulf of Alaska from 2018 to 2020 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/936203.rdf Name: OrgSequence Units: unitless Description: <p>Internal reference (numeric sequential identifier)</p> http://lod.bco-dmo.org/id/dataset-parameter/936204.rdf Name: Year Units: unitless Description: <p>Year of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/936205.rdf Name: Season Units: unitless Description: <p>Seasons of sample (spring, summer, fall) </p> http://lod.bco-dmo.org/id/dataset-parameter/936206.rdf Name: station Units: unitless Description: <p>NGA-LRTER station name</p> http://lod.bco-dmo.org/id/dataset-parameter/936207.rdf Name: DateTime_local Units: unitless Description: <p>Date and time of samples (Alaska local time AKST/AKDT). The format is excel default time base.  </p> http://lod.bco-dmo.org/id/dataset-parameter/936208.rdf Name: ISO_DateTime_UTC Units: unitless Description: <p>Date and time of samples in ISO 8601 format with timezone (UTC).</p> http://lod.bco-dmo.org/id/dataset-parameter/936209.rdf Name: Latitude Units: decimal degrees Description: <p>Latitude</p> http://lod.bco-dmo.org/id/dataset-parameter/936210.rdf Name: Longitude Units: decimal degrees Description: <p>Longitude</p> http://lod.bco-dmo.org/id/dataset-parameter/936211.rdf Name: DephMax Units: meters (m) Description: <p>Maximum depth of sample in meters</p> http://lod.bco-dmo.org/id/dataset-parameter/936212.rdf Name: DepthMin Units: meters (m) Description: <p>Minimum depth of samples in meters</p> http://lod.bco-dmo.org/id/dataset-parameter/936213.rdf Name: species Units: unitless Description: <p>Species of Neocalanus (see Methodology for taxon ids)</p> http://lod.bco-dmo.org/id/dataset-parameter/936214.rdf Name: LifeStage Units: unitless Description: <p>Copepodite life stage</p> http://lod.bco-dmo.org/id/dataset-parameter/936215.rdf Name: ProsomeLength Units: micrometers (um) Description: <p>Prosome length</p> http://lod.bco-dmo.org/id/dataset-parameter/936216.rdf Name: ProsomeHeight Units: micrometers (um) Description: <p>Prosome height (at maxillipeds)</p> http://lod.bco-dmo.org/id/dataset-parameter/936217.rdf Name: LipidLength Units: micrometers (um) Description: <p>Lipid sac length</p> http://lod.bco-dmo.org/id/dataset-parameter/936218.rdf Name: LipidHeight Units: micrometers (um) Description: <p>Lipid sac height (at maxillipeds)</p> http://lod.bco-dmo.org/id/dataset-parameter/936219.rdf Name: LipidArea Units: square millimeters (mm^2) Description: <p>Lipid sac area in lateral view</p> http://lod.bco-dmo.org/id/dataset-parameter/936220.rdf Name: AdjustedHeight Units: micrometers (um) Description: <p>Lipid sac average height</p> http://lod.bco-dmo.org/id/dataset-parameter/936221.rdf Name: LipidVolume Units: cubic millimeters (mm^3) Description: <p>Lipid sac volume</p> http://lod.bco-dmo.org/id/dataset-parameter/936222.rdf Name: BodyVolume Units: cubic millimeters (mm^3) Description: <p>Body volume</p> http://lod.bco-dmo.org/id/dataset-parameter/936223.rdf Name: Lipid_percent_BodyVolume Units: percent (%) Description: <p>Percentage Lipids of total body volume</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact https://osprey.bco-dmo.org/dataset/936158/data/download download onLine dataset &lt;p&gt;&lt;!--[if gte mso 9]&gt;&lt;xml&gt; 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&lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;w&gt;&lt;/w:LsdException&gt; &lt;/w:LatentStyles&gt; &lt;/xml&gt;&lt;![endif]--&gt;&lt;!--[if gte mso 10]&gt; &lt;style&gt; /* Style Definitions */ table.MsoNormalTable {mso-style-name:&quot;Table Normal&quot;; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:&quot;&quot;; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-pagination:widow-orphan; font-size:12.0pt; font-family:&quot;Calibri&quot;,sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:&quot;Times New Roman&quot;; mso-bidi-theme-font:minor-bidi;} &lt;/style&gt; &lt;![endif]--&gt;&lt;/p&gt; &lt;p&gt;Zooplankton samples were collected on NGA-LTER cruises along the Seward Line and within PWS during the summer, spring, and fall of 2018-20 aboard the R/V &lt;em&gt;Sikuliaq&lt;/em&gt;, R/V &lt;em&gt;Tiglax&lt;/em&gt; or the R/V &lt;em&gt;Woldstad&lt;/em&gt;. During spring and summer cruises copepods were collected using a Quad net, a modified CalVet net (0.25 m mouth diameter, 1 m cylinder and 1.5 m cone), of 150-µm mesh hauled vertically from 100 m during the day, then live sorted and imaged. During summer 2019, animals were also collected during the night in the drogue net (0-200 m) of a towed Midi Multinet (0.25 m&lt;sup&gt;2&lt;/sup&gt;) (Hydro-Bios, Germany), equipped with 500-µm mesh nets, and live sorted and imaged the following morning. The towed Multinet fished five discrete depth layers including: 0-20, 20-40, 40-60, 60-100, and 100-200 m. During summer 2020, we employed a vertically-hauled Midi Multinet of 150-µm mesh (fished during the day) for these same strata that were live-sorted and imaged immediately after collection to improve specimen quality. During summer and fall of all years at one or two deep-water Seward Line stations, and one or two PWS stations, we also fished the vertically-hauled Multinet at four additional depths: 200-300, 300-400, 400-600 and 600-1200 m (or 600-720 for PWS). A flowmeter installed in each net quantified the volume of water filtered. We targeted &lt;em&gt;Neocalanus flemingeri&lt;/em&gt;, &lt;em&gt;Neocalanus plumchrus &lt;/em&gt;and&lt;em&gt; Neocalanus cristatus &lt;/em&gt;for sorting, staging, and imaging from each of these strata.&lt;/p&gt; &lt;p&gt;Up to 60 individuals of each stage for each species were analyzed, if present. The &lt;em&gt;Neocalanus&lt;/em&gt; specimens were selected individually from homogenized temperature-regulated subsamples using a ladle or wide-bore plastic pipette. Only living, apparently undamaged individuals with intact lipid sacs were sorted and imaged using a Leica MZ16 microscope with either a Spot 4Mpx, Jenoptik 8Mpx, or Spot 12Mpx digital camera. Depending on stage and species, just prior to imaging three to fifteen animals were placed in a chilled embryo dish then water was reduced so animals shifted onto their sides (with gentle manipulation as needed). For every magnification setting, a calibration scale bar was added to the images. In cases where availability of animals was limited, animals with compromised lipid sacs were imaged to establish prosome length only. The calibrated images were later analyzed using Spot Software (V4.7 or 5.3) for determination of the prosome length (measured as maximum) and prosome width (measured from top of prosome to base of maxillipeds), the lipid sac length (measured from most anterior point to most posterior point), and lipid sac width (measured starting at the point closest to the base of the maxillipeds (ventral point) to most dorsal point). Animals that showed signs of compromised lipid sacs upon image inspection were only measured for prosome length. Adobe Photoshop (version CS6) was utilized to measure lipid sac area using the quick select tool to help demarcate the perimeter of the lipid sac. The number of pixels within the perimeter of the lipid sac was converted to area (mm&lt;sup&gt;2&lt;/sup&gt;) using the pixel-to-µm calibration associated with each image.&lt;/p&gt; &lt;p&gt;To calculate lipid sac volume, we first assumed the lipid sac to be a cylinder. We used the measured lipid sac length and the measured lipid sac area to find the average diameter of the cylinder (i.e. area divided by length) in order to account for the varying height and shape of the lipid sac. We then used this average diameter to estimate the volume of the lipid sac.&amp;nbsp; Body volume wa calcualted at a oblate spheroid from prosome length and prosome height.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Species List:&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Scientfic_Name, Lifesciences Identifier (LSID)&lt;/strong&gt;&lt;br /&gt; Neocalanus flemingeri, urn:lsid:marinespecies.org:taxname:353708&lt;br /&gt; Neocalanus plumchrus, urn:lsid:marinespecies.org:taxname:196772&lt;br /&gt; Neocalanus cristatus, urn:lsid:marinespecies.org:taxname:104470&lt;/p&gt; Specified by the Principal Investigator(s) BCO-DMO Data Manager Processing Notes: * Data from source file lipid.data.final 2024.csv were imported into the BCO-DMO data system. csv downloads of these data will have the name &quot;936158_v1_neocalanus-lipids-and-morphometrics.csv.&quot; * Parameters (column names) renamed to comply with BCO-DMO naming conventions. See https://www.bco-dmo.org/page/bco-dmo-data-processing-conventions * ISO_DateTime_UTC column added. Converted from DateTime (Alaska local time AKST/AKDT in excel numeric format) to human readable date in ISO 8601 format timestamp with timezone (UTC as Z). * decimals in columns (LipidArea, AdjustedHeight, LipidVolume, BodyVolume, Lipid_percent_BodyVolume) greater than 4 decimal places were rounded to 4 decimal places. * Missing data in the BCO-DMO system displays as a blank (null values) by default and will vary depending upon the file format downloaded (blank in csv files, NaN in .mat matlab files, etc). Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Hydrobios Multinet-midi both 150um and 500um mesh Hydrobios Multinet-midi both 150um and 500um mesh PI Supplied Instrument Name: Hydrobios Multinet-midi both 150um and 500um mesh Instrument Name: MultiNet Instrument Short Name:MultiNet Instrument Description: The MultiNet© Multiple Plankton Sampler is designed as a sampling system for horizontal and vertical collections in successive water layers. Equipped with 5 or 9 net bags, the MultiNet© can be delivered in 3 sizes (apertures) : Mini (0.125 m2), Midi (0.25 m2) and Maxi (0.5 m2). The system consists of a shipboard Deck Command Unit and a stainless steel frame to which 5 (or 9) net bags are attached by means of zippers to canvas. The net bags are opened and closed by means of an arrangement of levers that are triggered by a battery powered Motor Unit. The commands for actuation of the net bags are given via single or multi-conductor cable between the Underwater Unit and the Deck Command Unit. Although horizontal collections typically use a mesh size of 300 microns, mesh sizes from 100 to 500 may also be used. Vertical collections are also common. The shipboard Deck Command Unit displays all relevant system data, including the actual operating depth of the net system. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/NETT0099/ PI Supplied Instrument Name: PI Supplied Instrument Description: Quad net, a modified CalVet net (0.25 m mouth diameter, 1 m cylinder and 1.5 m cone), of 150-µm mesh Instrument Name: Plankton Net Instrument Short Name:Plankton Net Instrument Description: A Plankton Net is a generic term for a sampling net that is used to collect plankton. It is used only when detailed instrument documentation is not available. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/22/ Digital cameras Digital cameras PI Supplied Instrument Name: Digital cameras Instrument Name: Underwater Camera Instrument Short Name: Instrument Description: All types of photographic equipment that may be deployed underwater including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/180/ Cruise: SKQ201810S SKQ201810S R/V Sikuliaq Community Standard Description International Council for the Exploration of the Sea R/V Sikuliaq vessel SKQ201810S Russell R. Hopcroft https://nga.lternet.edu/wp-content/uploads/2019/04/Cruise-Report-SKQ201810S.pdf Report describing SKQ201810S Cruise: SKQ201915S SKQ201915S R/V Sikuliaq Community Standard Description International Council for the Exploration of the Sea R/V Sikuliaq vessel SKQ201915S Seth L. Danielson University of Alaska Fairbanks https://nga.lternet.edu/wp-content/uploads/2020/03/Cruise-Report-SKQ201915S_v3.pdf Report describing SKQ201915S Cruise: SKQ202006S SKQ202006S R/V Sikuliaq Community Standard Description International Council for the Exploration of the Sea R/V Sikuliaq vessel SKQ202006S Russell R. Hopcroft https://nga.lternet.edu/wp-content/uploads/2020/07/Cruise-Report-SKQ202006S_v2.pdf Report describing SKQ202006S Cruise: SKQ202010S SKQ202010S R/V Sikuliaq Community Standard Description International Council for the Exploration of the Sea R/V Sikuliaq vessel SKQ202010S https://nga.lternet.edu/wp-content/uploads/2020/12/Cruise-Report-SKQ202010S.pdf Report describing SKQ202010S Cruise: SKQ202012S SKQ202012S R/V Sikuliaq Community Standard Description International Council for the Exploration of the Sea R/V Sikuliaq vessel SKQ202012S Russell R. Hopcroft https://nga.lternet.edu/wp-content/uploads/2020/11/Cruise-Report-SKQ202012S.pdf Report describing SKQ202012S Cruise: WSD201807 WSD201807 R/V Woldstad Community Standard Description International Council for the Exploration of the Sea R/V Woldstad vessel WSD201807 Russell R. Hopcroft University of Alaska Fairbanks https://nga.lternet.edu/wp-content/uploads/2019/04/Cruise-Report-WOL18J.pdf Report describing WSD201807 Cruise: TXS19 TXS19 R/V Tiglax Community Standard Description International Council for the Exploration of the Sea R/V Tiglax vessel TXS19 Russell R. Hopcroft University of Alaska Fairbanks https://nga.lternet.edu/wp-content/uploads/2019/10/Cruise-Report-TXS19.pdf Report describing TXS19 Cruise: TXF19 TXF19 R/V Tiglax Community Standard Description International Council for the Exploration of the Sea R/V Tiglax vessel TXF19 Russell R. Hopcroft University of Alaska Fairbanks https://nga.lternet.edu/wp-content/uploads/2020/02/Cruise-Report-TXF19.pdf Report describing TXF19 Cruise: TXF18 TXF18 R/V Tiglax Community Standard Description International Council for the Exploration of the Sea R/V Tiglax vessel TXF18 Russell R. Hopcroft University of Alaska Fairbanks https://nga.lternet.edu/wp-content/uploads/2019/04/Cruise-Report-TXF18.pdf Report describing TXF18 R/V Sikuliaq Community Standard Description International Council for the Exploration of the Sea R/V Sikuliaq vessel R/V Woldstad Community Standard Description International Council for the Exploration of the Sea R/V Woldstad vessel R/V Tiglax Community Standard Description International Council for the Exploration of the Sea R/V Tiglax vessel