http://lod.bco-dmo.org/id/dataset/936849
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-09-09
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Amplicon sequence variants (ASVs) and taxonomy of Pseudo-nitzschia spp. from Narragansett Bay in Rhode Island, USA and the Northeast U.S. Shelf (NES-LTER transect) from 2018-2023
2024-10-11
publication
2024-10-11
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-10-14
publication
https://doi.org/10.26008/1912/bco-dmo.936849.1
Bethany D. Jenkins
University of Rhode Island
principalInvestigator
Matthew Bertin
University of Rhode Island
principalInvestigator
Riley Kirk
University of Rhode Island
principalInvestigator
Tatiana A. Rynearson
University of Rhode Island
principalInvestigator
Alexa Sterling
University of Rhode Island
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Roche, K. M., Church, I., Sterling, A., Rynearson, T. A., Bertin, M., Kim, A., Kirk, R., Jenkins, B. D. (2024) Amplicon sequence variants (ASVs) and taxonomy of Pseudo-nitzschia spp. from Narragansett Bay in Rhode Island, USA and the Northeast U.S. Shelf (NES-LTER transect) from 2018-2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-10-11 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.936849.1 [access date]
Dataset Description: <p>Acknowledgement:</p>
<p>We acknowledge the NSF RI C-AIM EPSCoR Cooperative Agreement (OIA-1004057) for research support. Sequencing was performed at the University of Rhode Island Molecular Informatics Core supported by the Institutional Development Award (IDeA) Network for Biomedical Research Excellence from the National Institute of General Medical Sciences of the National Institutes of Health (P20GM103430).</p> Methods and Sampling: <p><span style="font-size:14px">Samples were selected from the NES-LTER transect and various time series sites in </span><span style="color:rgb(0, 0, 0); font-family:helvetica,arial,sans-serif; font-size:14.4px">Narragansett Bay, Rhode Island (</span><span style="font-size:14px">NB) during winter and summer periods from January 2018 through February 2023 to compare seasonal and regional patterns of <em>Pseudo-nitzschia</em> species composition and DA, as well as environmental drivers. NB and NES will henceforth be referred to as subregions of the larger Northeast U.S. Continental Shelf region, with NES specifically referring to the area spanned by the NES-LTER transect. Samples were collected on NES-LTER cruises (<em>R/V</em> Endeavor, <em>R/V</em> Atlantis) from 11 stations along a 150 km transect (n=77) each winter (January-February) and summer (July-August). Samples from three to four stations per cruise were used in this dataset spanning innershelf (L1), midshelf (L3, L4) and outershelf (L7, L8, L10) sections of the transect. The northernmost station, L1, is about 50 km from the mouth of NB. To collect plankton biomass for nucleic acid isolation, CTD rosette seawater from the surface and subsurface chlorophyll maximum (SCM) were passed via peristaltic pump over 25 mm 5 µm pore size filters (Sterlitech, Kent, WA, USA). Biomass filters were either flash frozen in liquid nitrogen (2018-2022) or placed in DNA/RNA shield (winter 2023; Zymo Research, Irvine, CA, USA) and stored in a -80°C freezer. The SCM depth varied as observed by <em>in situ</em> chlorophyll fluorescence, with a median depth of 28 m for summer and 19 m for winter samples. In cases where the SCM was not well defined due to water column mixing that typically took place in winter at nearshore stations, a sampling depth between 20 and 30 m was targeted.</span></p>
<p>&nbsp;</p>
<p><span style="font-size:14px">In NB, surface seawater samples were collected from various sites in the East and West Passages including the Narragansett Bay Long-Term Plankton Time Series (NBPTS) site, Whale Rock (WR), Castle Hill Beach (CHB), East Passage (EP), and University of Rhode Island Graduate School of Oceanography (GSO) dock. Seawater was transported back to the laboratory and passed over 25 mm 5 µm pore size filters (Sterlitech, Kent, WA, USA) using a peristaltic pump before flash freezing in liquid nitrogen and storage at -80°C. To fill in several missing dates from this time series, six samples collected separately in the NBPTS (https://web.uri.edu/gso/research/plankton/) sampling program were used. These samples differed in collection methodology only by the filter pore size used (0.22 µm, Express Plus, Millipore Sigma) and vacuum as opposed to peristaltic filtration.</span></p>
<p>&nbsp;</p>
<p><span style="font-size:14px">DNA was extracted from most NB and NES samples (n=219) using a modified version of the DNeasy Plant Kit (Qiagen, Germantown, MD, USA) that included a 1-minute bead beating step (0.1 mm and 0.5 mm Zirconia/Silica beads, BioSpec Products, Bartlesville, OK, USA) and two part elution into a total of 45 µL Buffer AE. Similarly, the six NBPTS samples were extracted using a modified version of the DNeasy Blood &amp; Tissue kit (Qiagen, Germantown, MD, USA) with a 1-minute bead beating step and final elution into 50 µL Buffer AE. Some NES samples (n=18) were extracted using the Quick-DNA/RNA Miniprep Plus Kit (Zymo Research, Irvine, CA, USA) with a 1-minute bead beating step (0.4 mm Zirconium Beads, OPS Diagnostics, Lebanon, NJ, USA) and final elution into 50 µL nuclease-free water. DNA from each sample was amplified with a primer set that targets the eukaryotic internal transcribed spacer region 1 (ITS1) and effectively distinguishes <em>Pseudo-nitzschia</em> species (White et al., 1990; Sterling et al., 2022). Briefly, DNA was diluted to 1-4 ng/µL and 2 µL of template was added to 25 µL PCR reactions with Phusion Hot Start High-Fidelity Master Mix (Thermo Fisher Scientific Inc., Waltham, MA, USA) and HPLC-purified forward and reverse primers at 0.5 µM concentration with Illumina MiSeq adapters (Integrated DNA Technologies, Coralville, IA, USA). A stepwise thermocycle was used as described in Sterling et al. (2022).</span></p>
<p>&nbsp;</p>
<p><span style="font-size:14px">DNA amplicons were sequenced at the Rhode Island-INBRE Molecular Informatics Core on the Illumina MiSeq platform (Illumina, Inc., San Diego, CA, USA). There, libraries were prepared by cleaning ITS1 PCR products with KAPA pure beads (KAPA Biosystems, Woburn, MA, USA) and attaching sequencing indices and adapters using PCR. This amplification was performed with the Illumina Nextera XT Index Kit (Illumina, San Diego, CA, USA) and Phusion High Fidelity Master Mix, followed by a second round of cleaning with KAPA pure beads and visualization with gel electrophoresis. The quality of select samples was assessed on a Bioanalyzer DNA1000 chip (Agilent Technologies, Santa Clara, CA, USA) and all samples were quantified on a Qubit fluorometer (Invitrogen, Carlsbad, CA, USA). The final library was pooled, quantified with qPCR on a LightCycler480 (Roche, Pleasanton, CA, USA) using a KAPA Biosystems Illumina Kit (KAPA Biosystems, Woburn, MA, USA), and sequenced on the Illumina MiSeq using v3 chemistry and 2x250 paired-end reads. Samples were sequenced across five separate MiSeq runs using identical methods and negative controls.</span></p>
<p>&nbsp;</p>
<p><span style="font-size:14px">Sampling Locations</span></p>
<p><span style="font-size:14px"><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">Narragansett Bay sites: </span></span></span></p>
<p><span style="font-size:14px"><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">Narragansett Bay Long Term Plankton Time Series (41.57 N -71.39 W)</span></span></span></p>
<p><span style="font-size:14px"><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">Whale Rock (41.43 N -71.42 W)</span></span></span></p>
<p><span style="font-size:14px"><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">East Passage (41.45 N -71.38 W)</span></span></span></p>
<p><span style="font-size:14px"><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">Castle Hill Beach (41.46 N -71.36 W)</span></span></span></p>
<p><span style="font-size:14px"><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">Graduate School of Oceanography dock (41.49 N -71.42 W)</span></span></span></p>
<p><span style="font-size:14px"><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">NES stations: </span></span></span></p>
<p><span style="font-size:14px"><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">L1 (41.20 N -70.88 W), L3 (40.86 N -70.88 W), L4 (40.70 N -70.88 W), L7 (40.23 N -70.88 W), L8 (40.14 N -70.77 W), L10 (39.93 N -70.88 W)</span></span></span></p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1655686 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1655686
Funding provided by NSF Office of Integrative Activities (NSF OIA) Award Number: OIA-1655221 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1655221
Funding provided by National Oceanic and Atmospheric Administration (NOAA) Award Number: NA18OAR4170094
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2322676 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2322676
completed
Bethany D. Jenkins
University of Rhode Island
401-874-7551
Department of Cell and Molecular Biology and Graduate School of Oceanography 279 CBLS, 120 Flagg Road
Kingston
RI
02881
USA
bdjenkins@uri.edu
pointOfContact
Matthew Bertin
University of Rhode Island
401 874 5016
mbertin@uri.edu
pointOfContact
Riley Kirk
University of Rhode Island
rileykirk@uri.edu
pointOfContact
Tatiana A. Rynearson
University of Rhode Island
401-874-6022
Graduate School of Oceanography, University of Rhode Island 215 South Ferry Rd.
Narragansett
RI
02882
USA
rynearson@uri.edu
pointOfContact
Alexa Sterling
University of Rhode Island
Sterling.Alexa@epa.gov
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Species
Assignment_method
NCBI_GenBank_Accession_Number
Genbank_ASV_number
ASV_seq
BioProject
Illumina MiSeq Next Generation Sequencing
CTD rosette
peristaltic pump
LightCycler480
Qubit fluorometer
Eppendorf Mastercycler EP Thermal Cycler Series
theme
None, User defined
species scientific name (binomial)
No BCO-DMO term
accession number
sequence
NCBI BioProject accession
featureType
BCO-DMO Standard Parameters
Automated DNA Sequencer
CTD - profiler
Pump
qPCR Thermal Cycler
Qubit fluorometer
Thermal Cycler
instrument
BCO-DMO Standard Instruments
EN608
EN617
EN627
EN644
EN649
EN655
EN661
EN668
EN687
EN695
AT46
service
Deployment Activity
Narragansett Bay in Rhode Island, USA and offshore Atlantic Ocean transect across the Northeast U.S. Shelf (NES)
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Long Term Ecological Research network
http://www.lternet.edu/
Long Term Ecological Research network
adapted from http://www.lternet.edu/
The National Science Foundation established the LTER program in 1980 to support research on long-term ecological phenomena in the United States. The Long Term Ecological Research (LTER) Network is a collaborative effort involving more than 1800 scientists and students investigating ecological processes over long temporal and broad spatial scales. The LTER Network promotes synthesis and comparative research across sites and ecosystems and among other related national and international research programs. The LTER research sites represent diverse ecosystems with emphasis on different research themes, and cross-site communication, network publications, and research-planning activities are coordinated through the LTER Network Office.
2017 LTER research site map obtained from https://lternet.edu/site/lter-network/
LTER
largerWorkCitation
program
Northeast U.S. Shelf Long Term Ecological Research site
https://nes-lter.whoi.edu/
Northeast U.S. Shelf Long Term Ecological Research site
<p><strong>Continuing Award OCE-2322676<br />
<em>Sep 2023 to Aug 2028 (estimated)</em><br />
LTER: Scales of Variability in Ecosystem Dynamics and Production on the Changing Northeast U.S. Shelf (NES II)</strong><br />
<strong><em>NSF Award Abstract:</em></strong></p>
<p>The Northeast U.S. Shelf (NES) is the region of the Northwest Atlantic Ocean that overlies the continental shelf from North Carolina to Maine. The NES has a long history of intense human utilization and provides an array of ecosystem services including shipping, recreation, conservation, and energy development. The NES also comprises a seasonally dynamic and productive ecosystem, supporting renowned fisheries, whose integrity is critical to the health of the Northeast U.S. economy. The NES ecosystem's productivity is fueled by planktonic organisms that interact with each other in complex food webs whose structure depends on environmental conditions (e.g., temperature, light, and nutrient levels). These conditions are rapidly changing because of climate-change-related warming and human utilization. For example, the NES is seeing the largest development of coastal wind farms in the U.S. to date. Phase II of the Northeast U.S. Shelf Long-Term Ecological Research program (NES-LTER II) advances our ability to predict how anthropogenic impacts will affect the dynamics of the shelf's planktonic food webs and their ability to support the productivity of higher trophic levels, from fish to whales and humans. Because the NES is subject to long-term challenges that will impact many people, the project emphasizes an active education component for helping to train the next generation of marine scientists and outreach activities to increase public understanding of marine science and technology. The project team conducts education and outreach via three main components: (1) training and mentoring for early career researchers from undergraduates to postdoctoral researchers in LTER research; (2) an LTER Schoolyard program that engages middle and high school teachers and students; and (3) public outreach through targeted events, the project website, and social media channels.</p>
<p>Patterns of ecosystem change over seasons to decades have been documented in the NES, but the key mechanisms linking changes in the physical environment, planktonic food webs, and higher trophic levels remain poorly understood. As a result, predictive capability is limited and management strategies are largely reactive. To address these needs, NES II is targeting a mechanistic understanding of how food web structure and function responds to environmental conditions, natural variability and human induced changes. NES II combines observations that provide regional-scale context, process cruises along a high gradient cross-shelf transect, high-frequency time series at an inner-shelf location, coupled biological-physical food web models, and targeted population models. In addition, the research team is investigating how community structure and trophic transfer are impacted by disturbances including (i) the increasing prevalence of heat waves, (ii) intrusions of offshore water associated with increasing instability in the Gulf Stream, and (iii) offshore wind farms now under construction on the NES. The long-term research plan is guided by the overarching science question: "How is climate change impacting the pelagic NES ecosystem and, in particular, affecting the relationship between compositional (e.g., species diversity and size structure) and aggregate (e.g., rates of primary production, and transfer of energy to higher trophic levels) variability?" The investigators are assessing the extent to which the NES ecosystem possesses a biodiversity reservoir that is resilient to dramatic changes in the environment and that will allow the ecosystem to maintain overall productivity.<br />
</p>
<p><strong>Prior Award<br />
<em>Sep 2017 to Feb 2024</em><br />
LTER: Linking Pelagic Community Structure with Ecosystem Dynamics and Production Regimes on the Changing Northeast US Shelf</strong><em> </em><br />
Summary information including abstract, PIs, and other award details are included in the Funding History PDF in the Files section below.</p>
<p><strong>Additional Information:</strong><br />
The NES-LTER project includes collaboration with the National Marine Fisheries Service / Northeast Fisheries Science Center [NMFS/NEFSC] in particular for sharing data related to Project EcoMon Zooplankton <a class="moz-txt-link-freetext" href="https://www.bco-dmo.org/project/2106">https://www.bco-dmo.org/project/2106.</a></p>
<p><strong>This project is supported by continuing grants with slight name variations:</strong></p>
<ul>
<li><span style="color:rgb(0, 0, 0); font-family:helvetica,arial,sans-serif; font-size:14.4px"> LTER: Linking Pelagic Community Structure with Ecosystem Dynamics and Production Regimes on the Changing Northeast US Shelf</span></li>
<li><span style="color:rgb(0, 0, 0); font-family:helvetica,arial,sans-serif; font-size:14.4px"> LTER: Scales of Variability in Ecosystem Dynamics and Production on the Changing Northeast U.S. Shelf (NES II)</span></li>
</ul>
NES LTER
largerWorkCitation
project
RII Track-1: Rhode Island Consortium for Coastal Ecology Assessment, Innovation, and Modeling
https://osprey.bco-dmo.org/project/836631
RII Track-1: Rhode Island Consortium for Coastal Ecology Assessment, Innovation, and Modeling
<p>NSF Award Abstract:</p>
<p>Non-technical Description<br />
The University of Rhode Island (URI) will establish the Consortium for Coastal Ecology Assessment, Innovation, and Modeling (C-AIM) to coordinate research, education, and workforce development across Rhode Island (RI) in coastal marine science and ecology. C-AIM addresses fundamental research questions using observations, computational methods, and technology development applied to Narraganset Bay (NB), the largest estuary in New England and home to important ecosystem services including fisheries, recreation, and tourism. The research will improve understanding of the microorganisms in NB, develop new models to predict pollution and harmful algal bloom events in NB, build new sensors for nutrients and pollutants, and provide data and tools for stakeholders in the state. Observational capabilities will be coordinated in an open platform for researchers across RI; it will provide real-time physical, chemical, and biological observations ? including live streaming to mobile devices. C-AIM will also establish the RI STEAM (STEM + Art) Imaging Consortium to foster collaboration between artists, designers, engineers, and scientists. Research internships will be offered to undergraduate students throughout the state and seed funding for research projects will be competitively awarded to Primarily Undergraduate Institution partners.</p>
<p>Technical Description<br />
C-AIM will employ observations and modeling to assess interactions between organisms and ecosystem function in NB and investigate ecological responses to environmental events, such as hypoxia and algal blooms. Observations of the circulation, biogeochemistry, and ecosystem will be made using existing and new instrument platforms. The Bay Observatory ? a network of observational platforms around NB - will be networked to trigger enhanced water sampling and sensing during specific environmental events, such as hypoxic conditions or phytoplankton blooms. Biogeochemical, ecological, and coastal circulation models will be integrated and coupled to focus on eutrophication and pollutant loading. Data and models will be integrated on multiple scales, from individual organisms and trophic interactions to food-web responses, and from turbulence to the regional ocean circulation. New sensing technologies for nutrients and pollutants will be developed, including affordable, micro-fluidic (Lab-on-a-Chip) devices with antifouling capabilities. The results will be synthesized and communicated to stakeholders.</p>
C-AIM
largerWorkCitation
project
Narragansett Bay Long-Term Plankton Time Series
https://web.uri.edu/gso/research/plankton/
Narragansett Bay Long-Term Plankton Time Series
<p>The Narragansett Bay Long-Term Plankton Time Series is one of the world’s longest-running plankton surveys. Beginning in 1957, weekly samples have been collected to assess the phytoplankton community and characterize the physical parameters of Narragansett Bay.</p>
<p>Samples are collected once per week -regardless of tidal stage- for temperature, salinity, turbidity, size-fractionated chlorophyll a and nutrients. Microplankton community composition (size range >10μm, both species identification and abundance) is determined using a light microscope to quantify live samples. The species list for the >10μm size fraction includes 246 different species or species complexes of protists. Samples are also collected for the determination of copepod and ctenophore concentrations.</p>
<p>Funding for the time series has come from the University of Rhode Island since 1999. Ship time is frequently provided by the U.S. Department of Fish and Wildlife.</p>
<p><strong>This Time Series is related to the following projects at BCO-DMO:</strong></p>
<ul>
<li>Connecting local, regional and global scales of gene flow in planktonic marine diatoms (<a href="https://www.bco-dmo.org/project/511708">https://www.bco-dmo.org/project/511708</a>)</li>
<li>Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients (<a href="https://www.bco-dmo.org/project/712787">https://www.bco-dmo.org/project/712787</a>)</li>
<li>LTER: Linking Pelagic Community Structure with Ecosystem Dynamics and Production Regimes on the Changing Northeast US Shelf (<a href="https://www.bco-dmo.org/project/747769">https://www.bco-dmo.org/project/747769</a>)</li>
<li>Quantifying Temperature Dependence In Growth & Grazing Rates of Planktonic Herbivores (<a href="https://www.bco-dmo.org/project/739232">https://www.bco-dmo.org/project/739232</a>)</li>
<li>RII Track-1: Rhode Island Consortium for Coastal Ecology Assessment, Innovation, and Modeling (<a href="https://www.bco-dmo.org/project/836631">https://www.bco-dmo.org/project/836631</a>)</li>
</ul>
NBPTS
largerWorkCitation
project
eng; USA
oceans
Narragansett Bay in Rhode Island, USA and offshore Atlantic Ocean transect across the Northeast U.S. Shelf (NES)
-71.42
-70.77
39.93
41.57
2018-01-31
2023-01-16
From projects that focused on the following 2 locations: 1. Northeast U.S. Continental Shelf Large Marine Ecosystem: 35.2019 to 46.0906 latitude, -77.3492 to -63.3608 longitude 2. Narragansett Bay, Rhode Island
0
BCO-DMO catalogue of parameters from Amplicon sequence variants (ASVs) and taxonomy of Pseudo-nitzschia spp. from Narragansett Bay in Rhode Island, USA and the Northeast U.S. Shelf (NES-LTER transect) from 2018-2023
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/940249.rdf
Name: Species
Units: unitless
Description: <p>Taxonomic assignment of Pseudo-nitzschia species</p>
http://lod.bco-dmo.org/id/dataset-parameter/940250.rdf
Name: Assignment_method
Units: unitless
Description: <p>Method used for taxonomic assignment. QIIME2 indicates the QIIME2 scikit-learn naïve Bayes machine learning classifier was used with a curated Pseudo-nitzschia species database to assign taxonomy. Megablast means that an Amplicon Sequence Variant (ASV) was assigned using a megablast search of NCBI's nr database with a threshold of >99% percent identity and >99% query coverage.</p>
http://lod.bco-dmo.org/id/dataset-parameter/940251.rdf
Name: NCBI_GenBank_Accession_Number
Units: unitless
Description: <p>GenBank accession number associated with an Amplicon Sequence Variant (ASV)</p>
http://lod.bco-dmo.org/id/dataset-parameter/940252.rdf
Name: Genbank_ASV_number
Units: unitless
Description: <p>Amplicon Sequence Variant (ASV) number assigned during GenBank submission</p>
http://lod.bco-dmo.org/id/dataset-parameter/940253.rdf
Name: ASV_seq
Units: unitless
Description: <p>DNA sequence of Amplicon Sequence Variant (ASV)</p>
http://lod.bco-dmo.org/id/dataset-parameter/940254.rdf
Name: BioProject
Units: unitless
Description: <p>NCBI BioProject accession number</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
66009
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/03444471-1652-4369-890f-e1da4e16e7d5/content
download
https://doi.org/10.26008/1912/bco-dmo.936849.1
download
onLine
dataset
<p><span style="font-size:14px">Samples were selected from the NES-LTER transect and various time series sites in </span><span style="color:rgb(0, 0, 0); font-family:helvetica,arial,sans-serif; font-size:14.4px">Narragansett Bay, Rhode Island (</span><span style="font-size:14px">NB) during winter and summer periods from January 2018 through February 2023 to compare seasonal and regional patterns of <em>Pseudo-nitzschia</em> species composition and DA, as well as environmental drivers. NB and NES will henceforth be referred to as subregions of the larger Northeast U.S. Continental Shelf region, with NES specifically referring to the area spanned by the NES-LTER transect. Samples were collected on NES-LTER cruises (<em>R/V</em> Endeavor, <em>R/V</em> Atlantis) from 11 stations along a 150 km transect (n=77) each winter (January-February) and summer (July-August). Samples from three to four stations per cruise were used in this dataset spanning innershelf (L1), midshelf (L3, L4) and outershelf (L7, L8, L10) sections of the transect. The northernmost station, L1, is about 50 km from the mouth of NB. To collect plankton biomass for nucleic acid isolation, CTD rosette seawater from the surface and subsurface chlorophyll maximum (SCM) were passed via peristaltic pump over 25 mm 5 µm pore size filters (Sterlitech, Kent, WA, USA). Biomass filters were either flash frozen in liquid nitrogen (2018-2022) or placed in DNA/RNA shield (winter 2023; Zymo Research, Irvine, CA, USA) and stored in a -80°C freezer. The SCM depth varied as observed by <em>in situ</em> chlorophyll fluorescence, with a median depth of 28 m for summer and 19 m for winter samples. In cases where the SCM was not well defined due to water column mixing that typically took place in winter at nearshore stations, a sampling depth between 20 and 30 m was targeted.</span></p>
<p>&nbsp;</p>
<p><span style="font-size:14px">In NB, surface seawater samples were collected from various sites in the East and West Passages including the Narragansett Bay Long-Term Plankton Time Series (NBPTS) site, Whale Rock (WR), Castle Hill Beach (CHB), East Passage (EP), and University of Rhode Island Graduate School of Oceanography (GSO) dock. Seawater was transported back to the laboratory and passed over 25 mm 5 µm pore size filters (Sterlitech, Kent, WA, USA) using a peristaltic pump before flash freezing in liquid nitrogen and storage at -80°C. To fill in several missing dates from this time series, six samples collected separately in the NBPTS (https://web.uri.edu/gso/research/plankton/) sampling program were used. These samples differed in collection methodology only by the filter pore size used (0.22 µm, Express Plus, Millipore Sigma) and vacuum as opposed to peristaltic filtration.</span></p>
<p>&nbsp;</p>
<p><span style="font-size:14px">DNA was extracted from most NB and NES samples (n=219) using a modified version of the DNeasy Plant Kit (Qiagen, Germantown, MD, USA) that included a 1-minute bead beating step (0.1 mm and 0.5 mm Zirconia/Silica beads, BioSpec Products, Bartlesville, OK, USA) and two part elution into a total of 45 µL Buffer AE. Similarly, the six NBPTS samples were extracted using a modified version of the DNeasy Blood &amp; Tissue kit (Qiagen, Germantown, MD, USA) with a 1-minute bead beating step and final elution into 50 µL Buffer AE. Some NES samples (n=18) were extracted using the Quick-DNA/RNA Miniprep Plus Kit (Zymo Research, Irvine, CA, USA) with a 1-minute bead beating step (0.4 mm Zirconium Beads, OPS Diagnostics, Lebanon, NJ, USA) and final elution into 50 µL nuclease-free water. DNA from each sample was amplified with a primer set that targets the eukaryotic internal transcribed spacer region 1 (ITS1) and effectively distinguishes <em>Pseudo-nitzschia</em> species (White et al., 1990; Sterling et al., 2022). Briefly, DNA was diluted to 1-4 ng/µL and 2 µL of template was added to 25 µL PCR reactions with Phusion Hot Start High-Fidelity Master Mix (Thermo Fisher Scientific Inc., Waltham, MA, USA) and HPLC-purified forward and reverse primers at 0.5 µM concentration with Illumina MiSeq adapters (Integrated DNA Technologies, Coralville, IA, USA). A stepwise thermocycle was used as described in Sterling et al. (2022).</span></p>
<p>&nbsp;</p>
<p><span style="font-size:14px">DNA amplicons were sequenced at the Rhode Island-INBRE Molecular Informatics Core on the Illumina MiSeq platform (Illumina, Inc., San Diego, CA, USA). There, libraries were prepared by cleaning ITS1 PCR products with KAPA pure beads (KAPA Biosystems, Woburn, MA, USA) and attaching sequencing indices and adapters using PCR. This amplification was performed with the Illumina Nextera XT Index Kit (Illumina, San Diego, CA, USA) and Phusion High Fidelity Master Mix, followed by a second round of cleaning with KAPA pure beads and visualization with gel electrophoresis. The quality of select samples was assessed on a Bioanalyzer DNA1000 chip (Agilent Technologies, Santa Clara, CA, USA) and all samples were quantified on a Qubit fluorometer (Invitrogen, Carlsbad, CA, USA). The final library was pooled, quantified with qPCR on a LightCycler480 (Roche, Pleasanton, CA, USA) using a KAPA Biosystems Illumina Kit (KAPA Biosystems, Woburn, MA, USA), and sequenced on the Illumina MiSeq using v3 chemistry and 2x250 paired-end reads. Samples were sequenced across five separate MiSeq runs using identical methods and negative controls.</span></p>
<p>&nbsp;</p>
<p><span style="font-size:14px">Sampling Locations</span></p>
<p><span style="font-size:14px"><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">Narragansett Bay sites: </span></span></span></p>
<p><span style="font-size:14px"><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">Narragansett Bay Long Term Plankton Time Series (41.57 N -71.39 W)</span></span></span></p>
<p><span style="font-size:14px"><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">Whale Rock (41.43 N -71.42 W)</span></span></span></p>
<p><span style="font-size:14px"><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">East Passage (41.45 N -71.38 W)</span></span></span></p>
<p><span style="font-size:14px"><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">Castle Hill Beach (41.46 N -71.36 W)</span></span></span></p>
<p><span style="font-size:14px"><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">Graduate School of Oceanography dock (41.49 N -71.42 W)</span></span></span></p>
<p><span style="font-size:14px"><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">NES stations: </span></span></span></p>
<p><span style="font-size:14px"><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">L1 (41.20 N -70.88 W), L3 (40.86 N -70.88 W), L4 (40.70 N -70.88 W), L7 (40.23 N -70.88 W), L8 (40.14 N -70.77 W), L10 (39.93 N -70.88 W)</span></span></span></p>
Specified by the Principal Investigator(s)
<p>Raw sequencing read quality was assessed using FastQC and MultiQC (v.0.11.9, v1.11) before and after primer and adapter trimming in Cutadapt (v3.2). The Divisive Amplicon Denoising Algorithm (DADA2) was used in R to estimate sequencing error and identify distinct amplicon sequence variants (ASVs) (v1.20.0). DADA2 was run separately for each sequencing run because it is designed to account for run-specific error. Taxonomy was assigned to ASVs from all sequencing runs using a dual approach to maximize the number of ASVs identified to the species level and enhance confidence. First, the scikit-learn naïve Bayes machine learning classifier in QIIME2 (v2022.11) and a curated reference database were used to assign taxonomy with a confidence threshold of 0.8. This curated database from Roche et al. (2022) included 302 unique <em>Pseudo-nitzschia </em>spp. ITS1 sequences from the National Center for Biotechnology Information (NCBI) GenBank with 51 different species represented (retrieved June 1, 2021). Next, to ensure that relevant <em>Pseudo-nitzschia </em>spp<em>.</em> ITS1 taxonomy was not omitted from the curated database, a megablast search was performed using the entire BLAST nt database. Additional ASVs classified by megablast were retained if there was &gt;99% identity and &gt;99% query cover to NCBI <em>Pseudo-nitzschia</em> species. If QIIME2 and megablast taxonomic assignment did not match for a particular ASV, no species-level taxonomy was assigned. Samples containing no reads identified to the <em>Pseudo-nitzschia</em> species level were removed from the analysis (n=15). To avoid potentially falsely detected taxa, ASVs classified as a species that appeared in only one sample across the dataset were discarded (n=6).</p>
Specified by the Principal Investigator(s)
1) Processed the submitted data file DATASET01_PN_ASVs_taxonomy.csv with the BCO-DMO tool Laminar.
Added a version number of 1 to the accession values in the column NCBI_GenBank_Accession_Numbe so that it is a GenBank accession value (which includes a version number).
Added a column named BioProject containing the BioProject accession number PRJNA1129077.
Saved the modified dataset to the file 936849_v1_pseudo_nitzschia_amplicon_seq_variant.csv.
2) Created a taxonomy table using the species names in the dataset and getting taxonomy values using the World Register of Marine Species (WoRMS) website and saved it to the file species_taxonomy.csv.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Illumina MiSeq Next Generation Sequencing
Illumina MiSeq Next Generation Sequencing
PI Supplied Instrument Name: Illumina MiSeq Next Generation Sequencing PI Supplied Instrument Description:Manufactured by Illumina, Inc., San Diego, CA, USA Instrument Name: Automated DNA Sequencer Instrument Short Name:Automated Sequencer Instrument Description: A DNA sequencer is an instrument that determines the order of deoxynucleotides in deoxyribonucleic acid sequences.
CTD rosette
CTD rosette
PI Supplied Instrument Name: CTD rosette Instrument Name: CTD - profiler Instrument Short Name: Instrument Description: The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.
This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
peristaltic pump
peristaltic pump
PI Supplied Instrument Name: peristaltic pump Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps
LightCycler480
LightCycler480
PI Supplied Instrument Name: LightCycler480 PI Supplied Instrument Description:Benchtop instrument. The LightCycler® 480 System features a versatile, plate-based, real-time PCR device that supports mono- or multicolor applications.
Manufactured by Roche, Pleasanton, CA, USA Instrument Name: qPCR Thermal Cycler Instrument Short Name:qPCR Instrument Description: An instrument for quantitative polymerase chain reaction (qPCR), also known as real-time polymerase chain reaction (Real-Time PCR).
Qubit fluorometer
Qubit fluorometer
PI Supplied Instrument Name: Qubit fluorometer PI Supplied Instrument Description:Manufactured by Invitrogen, Carlsbad, CA, USA Instrument Name: Qubit fluorometer Instrument Short Name: Instrument Description: Benchtop fluorometer. The Invitrogen Qubit Fluorometer accurately and quickly measures the concentration of DNA, RNA, or protein in a single sample. It can also be used to assess RNA integrity and quality.
Manufactured by Invitrogen, Carlsbad, CA, USA (Invitrogen is one of several brands under the Thermo Fisher Scientific corporation.)
Eppendorf Mastercycler EP Thermal Cycler Series
Eppendorf Mastercycler EP Thermal Cycler Series
PI Supplied Instrument Name: Eppendorf Mastercycler EP Thermal Cycler Series Instrument Name: Thermal Cycler Instrument Short Name:Thermal Cycler Instrument Description: A thermal cycler or "thermocycler" is a general term for a type of laboratory apparatus, commonly used for performing polymerase chain reaction (PCR), that is capable of repeatedly altering and maintaining specific temperatures for defined periods of time. The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps. They can also be used to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid diagnostics.
(adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)
Cruise: EN608
EN608
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN608
Heidi M. Sosik
Woods Hole Oceanographic Institution
Cruise: EN617
EN617
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN617
Heidi M. Sosik
Woods Hole Oceanographic Institution
Cruise: EN627
EN627
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN627
Heidi M. Sosik
Woods Hole Oceanographic Institution
Cruise: EN644
EN644
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN644
Heidi M. Sosik
Woods Hole Oceanographic Institution
Cruise: EN649
EN649
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN649
Heidi M. Sosik
Woods Hole Oceanographic Institution
Cruise: EN655
EN655
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN655
Heidi M. Sosik
Woods Hole Oceanographic Institution
Cruise: EN661
EN661
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN661
Heidi M. Sosik
Woods Hole Oceanographic Institution
Cruise: EN668
EN668
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN668
Heidi M. Sosik
Woods Hole Oceanographic Institution
Cruise: EN687
EN687
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN687
Heidi M. Sosik
Woods Hole Oceanographic Institution
Cruise: EN695
EN695
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN695
Heidi M. Sosik
Woods Hole Oceanographic Institution
Cruise: AT46
AT46
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT46
Heidi M. Sosik
Woods Hole Oceanographic Institution
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel