http://lod.bco-dmo.org/id/dataset/938004 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2024-09-19 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Adult Black Sea Bass (Centropristis striata) winter survival and lipid accumulation under varying diet and temperature conditions from a laboratory mesocosm experiment (Oct 2022 to Apr 2023) with individuals collected in Long Island Sound 2024-09-23 publication 2024-09-23 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2024-09-24 publication https://doi.org/10.26008/1912/bco-dmo.938004.1 Hannes Baumann University of Connecticut principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Baumann, H., Zavell, M. D. (2024) Adult Black Sea Bass (Centropristis striata) winter survival and lipid accumulation under varying diet and temperature conditions from a laboratory mesocosm experiment (Oct 2022 to Apr 2023) with individuals collected in Long Island Sound. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-09-23 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.938004.1 [access date] Methods and Sampling: &lt;p&gt;&lt;strong&gt;Experimental setup:&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;50 adult specimens were collected by boat off Stonington Borough, CT (41° 20’37.8” N 71° 54’51.4” W) between September 15th and October 10th, 2022. Individuals were collected via hook and line angling and were transported in aerated coolers to the Rankin seawater laboratory where adults were acclimated and equally split between two 3785-l tanks. Each tank was supplied with flowing, sand-filtered seawater from eastern LIS which naturally ranges in temperature from 25 to 4°C and a salinity between 28 to 33 ‰. Prior to the start of the experiment adults were fed previously frozen loligo squid (Seafreeze LLC, North Kingstown RI) 3× a week ad libitum.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Experimental design:&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;The adult experiment assessed the inshore overwintering potential of adult BSB under two distinct diets and a current inshore temperature regime for 200 days (October 10th, 2022, to April 28th, 2023). Following each specimen collection period, a subset of individuals was immediately euthanized with an overdose of MS-222 to act as a wild ‘pre-migratory’ baseline (‘fall’, n = 17) and dissected (“see methods in “Adult black sea bass winter survival and lipid dynamics: Wild fish”).&lt;/p&gt; &lt;p&gt;At the start of the experiment (October 10th, 2022) 50 specimens were briefly anesthetized, measured in length (TL = 32.8 ± 6.6 cm) and weight (wW = 514.7 ± 250), tagged with monofilament Floy-tags, and randomly allocated to one of two 3785-l tanks which was allocated a diet treatment; ‘mussels’ (Mytilus edulis; American Mussel Harvesters, North Kingston RI) and ‘herring’ (Clupea harrengus; Seafreeze ltd., North Kingston RI).&lt;/p&gt; &lt;p&gt;Fish were then fed 2% of the initial stocked biomass 3× a week throughout the experiment. Experimental tanks were flow-through (9060 l h-1), with conditions ambient to local LIS conditions and had a set of lights whose photoperiod was gradually changed weekly (11.5 h light: 12.5 h dark to 9 h light: 15 h dark and back up). Temperature was monitored in one tank by a Eureka Manta probe which recorded tank temperature every 30 minutes, while pH was monitored twice weekly via a handheld pH probe (Hach Handheld HQ40D), ammonia and salinity were measured once a week, and each tank was siphoned twice weekly to remove detritus. Over the course of the experiment there was no ammonia (0 ppm), while the mean pH and salinity were 8.04 ± 0.12 pH units and 31.8 ± 1.07 ‰, respectively.&lt;/p&gt; &lt;p&gt;Over the course of the experiment, fish were examined twice daily for mortalities or loss of equilibria. In the case of lost equilibria, specimens were immediately removed, euthanized with an overdose of MS-222, and dissected as previously described. Over the course of the experiment three individuals from the Herring diet treatment jumped overnight and weren’t included in subsequent analyses.&lt;/p&gt; &lt;p&gt;Since C. striata tend to have a high site-fidelity, beginning of April 2023, sampling occurred weekly on the same reef that the experimental and fall baseline specimens were collected on in order to collect ‘post-migratory’ baseline specimens (‘spring’, n = 21), upon their first entry into LIS (April 27th, 2023; see methods in “Adult black sea bass winter survival and lipid dynamics: Wild fish”). Specimens were immediately euthanized, measured, and dissected as previously described and the experiment ended the following day (April 28th, 2023). Upon ending the experiment all surviving specimens were measured for length (TL; 33.7 ± 4.4 cm), body depth (BD; 8 ± 0.9 cm), and wet weight (wW; 532.6 ± 227 g). During dissections, the stomach was removed to calculate a stomachless whole weight to standardize for consumed prey items, and the liver and gonad was then removed, individually weighed (0.01 g) and frozen at -20°C for future lipid extractions. A subsample of dorsally located white muscle tissue was also removed and frozen.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Response traits:&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;For experimental adults, we only calculated cumulative growth in length (GR), and specific growth rates (SGR). For all adults, we calculated gonadosomatic (GSI; %) and hepatosomatic (HSI; %) indices using stomachless fish mass (wW – stomach mass), to standardize for stomach contents, as {e.g., 100 × [(gonad or liver mass, g) / (stomachless fish mass)].&lt;/p&gt; &lt;p&gt;We quantified gonad, liver, and white muscle, storage lipid, lean-mass, and ash weights of each surviving experimental individual and baseline specimen. Samples were frozen at -50°C for 1 week and remeasured for whole body dry weight (dWb, 0.001 g). Following published protocols (Schultz and Conover 1997, Guo et al. 2021, 2022, Zavell et al. 2023), dried specimens were loaded into preweighed Alundum medium-porosity extraction thimbles and transferred to a custom-designed Soxhlet apparatus, where they were bathed in petroleum ether for 3.5 h to extract all metabolically available lipids. Samples were then dried overnight (60°C) and re-measured, with the change in pre- and post-extraction weights (ΔdW), representing the storage-lipid fraction (dWLipid). Samples were then muffle furnaced for 4 h at 550°C and reweighed with ΔdW, representing the lean-mass fraction (dWLean) and the remaining mass represents the inorganic fraction (dWAsh).&amp;nbsp;&lt;br /&gt; &lt;br /&gt; &lt;strong&gt;Organism Identifier (LSID, Life Sciences Identifier)&lt;/strong&gt;&lt;br /&gt; Centropristis striata, urn:lsid:marinespecies.org:taxname:159348&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1536336 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1536336 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756751 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756751 Funding provided by Connecticut Sea Grant (CTSG) Award Number: R/LR-30 completed Hannes Baumann University of Connecticut 860-405-9297 Department of Marine Sciences 1080 Shennecossett Road Groton CT 06340-6048 USA hannes.baumann@uconn.edu pointOfContact asNeeded Dataset Version: 1 Unknown Species Collection_Location Collection_Longitude Collection_Latitude Collection_Date Fish_ID Treatment Exp_Start_Date Exp_End_Date Mortality_Date Total_Exp_Duration Num_of_Days_Alive Survivor_or_Mortality TL0 TLF wW0 wWF GR_mm SGR Kwet0 KwetF stomach_wW stomachless_wW stomach_content_wW gonad_wW gonad_dW liver_wW liver_dW w_muscle_sub_dW Sex GSI HSI gonad_lipid_g gonad_lean_g liver_lipid_g liver_lean_g P_gonad_lipid P_gonad_lean P_liver_lipd P_liver_lean w_muscle_lipid_g w_muscle_lean_g p_w_muscle_lip p_w_muscle_lean Notes Hach Handheld pH and Temperature probe (HQ2200 Multi/2 Channel) Mettler Toledo Balance (XPR1202S) HOBO Pendant MX Water Temperature Data Logger theme None, User defined species scientific name (binomial) site longitude latitude date individual treatment time_elapsed mortality fish_len wet_weight_biomass growth sample description weight sex lipids in biota comments featureType BCO-DMO Standard Parameters Multi Parameter Portable Meter scale or balance Soxhlet extractor Temperature Logger instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative research: The genomic underpinnings of local adaptation despite gene flow along a coastal environmental cline https://befel.marinesciences.uconn.edu/2018/03/07/research-news-new-nsf-grant-to-study-silverside-genes/ Collaborative research: The genomic underpinnings of local adaptation despite gene flow along a coastal environmental cline <p>NSF Abstract:</p> <p>Oceans are large, open habitats, and it was previously believed that their lack of obvious barriers to dispersal would result in extensive mixing, preventing organisms from adapting genetically to particular habitats. It has recently become clear, however, that many marine species are subdivided into multiple populations that have evolved to thrive best under contrasting local environmental conditions. Nevertheless, we still know very little about the genomic mechanisms that enable divergent adaptations in the face of ongoing intermixing. This project focuses on the Atlantic silverside (Menidia menidia), a small estuarine fish that exhibits a remarkable degree of local adaptation in growth rates and a suite of other traits tightly associated with a climatic gradient across latitudes. Decades of prior lab and field studies have made Atlantic silverside one of the marine species for which we have the best understanding of evolutionary tradeoffs among traits and drivers of selection causing adaptive divergence. Yet, the underlying genomic basis is so far completely unknown. The investigators will integrate whole genome sequencing data from wild fish sampled across the distribution range with breeding experiments in the laboratory to decipher these genomic underpinnings. This will provide one of the most comprehensive assessments of the genomic basis for local adaptation in the oceans to date, thereby generating insights that are urgently needed for better predictions about how species can respond to rapid environmental change. The project will provide interdisciplinary training for a postdoc as well as two graduate and several undergraduate students from underrepresented minorities. The findings will also be leveraged to develop engaging teaching and outreach materials (e.g. a video documentary and popular science articles) to promote a better understanding of ecology, evolution, and local adaptation among science students and the general public.</p> <p>The goal of the project is to characterize the genomic basis and architecture underlying local adaptation in M. menidia and examine how the adaptive divergence is shaped by varying levels of gene flow and maintained over ecological time scales. The project is organized into four interconnected components. Part 1 examines fine-scale spatial patterns of genomic differentiation along the adaptive cline to a) characterize the connectivity landscape, b) identify genomic regions under divergent selection, and c) deduce potential drivers and targets of selection by examining how allele frequencies vary in relation to environmental factors and biogeographic features. Part 2 maps key locally adapted traits to the genome to dissect their underlying genomic basis. Part 3 integrates patterns of variation in the wild (part 1) and the mapping of traits under controlled conditions (part 2) to a) examine how genomic architectures underlying local adaptation vary across gene flow regimes and b) elucidating the potential role of chromosomal rearrangements and other tight linkage among adaptive alleles in facilitating adaptation. Finally, part 4 examines dispersal - selection dynamics over seasonal time scales to a) infer how selection against migrants and their offspring maintains local adaptation despite homogenizing connectivity and b) validate candidate loci for local adaptation. Varying levels of gene flow across the species range create a natural experiment for testing general predictions about the genomic mechanisms that enable adaptive divergence in the face of gene flow. The findings will therefore have broad implications and will significantly advance our understanding of the role genomic architecture plays in modifying the gene flow - selection balance within coastal environments.</p> <p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> GenomAdapt largerWorkCitation project eng; USA oceans -71.9332 -71.9332 41.3045 41.3045 2022-09-15 2023-04-28 Eastern coastline of North America 0 BCO-DMO catalogue of parameters from Adult Black Sea Bass (Centropristis striata) winter survival and lipid accumulation under varying diet and temperature conditions from a laboratory mesocosm experiment (Oct 2022 to Apr 2023) with individuals collected in Long Island Sound Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/938072.rdf Name: Species Units: unitless Description: <p>Black Sea Bass - Centropristis striata. LSID: urn:lsid:marinespecies.org:taxname:159348</p> http://lod.bco-dmo.org/id/dataset-parameter/938073.rdf Name: Collection_Location Units: unitless Description: <p>Stonington Borough, CT</p> http://lod.bco-dmo.org/id/dataset-parameter/938074.rdf Name: Collection_Longitude Units: decimal degrees Description: <p>Longitude of collection site (Stonington Borough)</p> http://lod.bco-dmo.org/id/dataset-parameter/938075.rdf Name: Collection_Latitude Units: decimal degrees Description: <p>Latitude of collection site (Stonington Borough)</p> http://lod.bco-dmo.org/id/dataset-parameter/938076.rdf Name: Collection_Date Units: unitless Description: <p>Date of fish collection in the wild</p> http://lod.bco-dmo.org/id/dataset-parameter/938077.rdf Name: Fish_ID Units: unitless Description: <p>ID of each individual fish</p> http://lod.bco-dmo.org/id/dataset-parameter/938078.rdf Name: Treatment Units: unitless Description: <p>Diet treatment (Mussels or Herring)</p> http://lod.bco-dmo.org/id/dataset-parameter/938079.rdf Name: Exp_Start_Date Units: unitless Description: <p>Date of experiment start</p> http://lod.bco-dmo.org/id/dataset-parameter/938080.rdf Name: Exp_End_Date Units: unitless Description: <p>Date of Experiment end</p> http://lod.bco-dmo.org/id/dataset-parameter/938081.rdf Name: Mortality_Date Units: unitless Description: <p>Date of an individual mortality</p> http://lod.bco-dmo.org/id/dataset-parameter/938082.rdf Name: Total_Exp_Duration Units: days Description: <p>Total duration of the experiment</p> http://lod.bco-dmo.org/id/dataset-parameter/938083.rdf Name: Num_of_Days_Alive Units: days Description: <p>Number of days an individual was alive</p> http://lod.bco-dmo.org/id/dataset-parameter/938084.rdf Name: Survivor_or_Mortality Units: unitless Description: <p>Denotes whether an individual survived (s) until the end of the experiment (200 days) or was a mortality (m) prior to the end of the expirement</p> http://lod.bco-dmo.org/id/dataset-parameter/938085.rdf Name: TL0 Units: centimenter (cm) Description: <p>Total length at experiment start</p> http://lod.bco-dmo.org/id/dataset-parameter/938086.rdf Name: TLF Units: centimenter (cm) Description: <p>Total length at at experiment end</p> http://lod.bco-dmo.org/id/dataset-parameter/938087.rdf Name: wW0 Units: grams (g) Description: <p>Whole body wet weight at experiment start</p> http://lod.bco-dmo.org/id/dataset-parameter/938088.rdf Name: wWF Units: grams (g) Description: <p>Whole body wet weight at experiment end</p> http://lod.bco-dmo.org/id/dataset-parameter/938089.rdf Name: GR_mm Units: millimeters per day (ml/day) Description: <p>Total length growth rate</p> http://lod.bco-dmo.org/id/dataset-parameter/938090.rdf Name: SGR Units: percent (%) per day Description: <p>Weight-specific growth rate</p> http://lod.bco-dmo.org/id/dataset-parameter/938091.rdf Name: Kwet0 Units: grams per cubic centimeter(g/cm3) Description: <p>Fulton's condition index using wet weight at experiment start. Kwet0 = (wW0 / TL0^3)*100 (see Fulton, TW (1902); Ricker, WE (1975))</p> http://lod.bco-dmo.org/id/dataset-parameter/938092.rdf Name: KwetF Units: grams per cubic centimeter(g/cm3) Description: <p>Fulton's condition index using wet weight at experiment end. KwetF = (wWF / TLF^3)*100 (see Fulton, TW (1902); Ricker, WE (1975)).</p> http://lod.bco-dmo.org/id/dataset-parameter/938093.rdf Name: stomach_wW Units: grams (g) Description: <p>Stomach weight at at experiment end</p> http://lod.bco-dmo.org/id/dataset-parameter/938094.rdf Name: stomachless_wW Units: grams (g) Description: <p>Stomachless weight at experiment end (wWF - Stomach_wW)</p> http://lod.bco-dmo.org/id/dataset-parameter/938095.rdf Name: stomach_content_wW Units: grams (g) Description: <p>Weight of stomach contants</p> http://lod.bco-dmo.org/id/dataset-parameter/938096.rdf Name: gonad_wW Units: grams (g) Description: <p>Gonad wet weight at experiment end</p> http://lod.bco-dmo.org/id/dataset-parameter/938097.rdf Name: gonad_dW Units: grams (g) Description: <p>Gonad dry weight at experiment end</p> http://lod.bco-dmo.org/id/dataset-parameter/938098.rdf Name: liver_wW Units: grams (g) Description: <p>Liver wet weight at experiment end</p> http://lod.bco-dmo.org/id/dataset-parameter/938099.rdf Name: liver_dW Units: grams (g) Description: <p>Liver dry weight at experiment end</p> http://lod.bco-dmo.org/id/dataset-parameter/938100.rdf Name: w_muscle_sub_dW Units: grams (g) Description: <p>Dry weight of the subsample of white muscle used to run lipid and lean analysis</p> http://lod.bco-dmo.org/id/dataset-parameter/938101.rdf Name: Sex Units: unitless Description: <p>Sex via visual observation of the gonads (M = male, F = female, U = unknown)</p> http://lod.bco-dmo.org/id/dataset-parameter/938102.rdf Name: GSI Units: percent (%) Description: <p>Gonadosomatic Index (gonad_wW / wWF *100)</p> http://lod.bco-dmo.org/id/dataset-parameter/938103.rdf Name: HSI Units: percent (%) Description: <p>Hepatosomatic Index (liver_wW / wWF *100)</p> http://lod.bco-dmo.org/id/dataset-parameter/938104.rdf Name: gonad_lipid_g Units: grams (g) Description: <p>Total gonad lipid content</p> http://lod.bco-dmo.org/id/dataset-parameter/938105.rdf Name: gonad_lean_g Units: grams (g) Description: <p>Total gonad lean content</p> http://lod.bco-dmo.org/id/dataset-parameter/938106.rdf Name: liver_lipid_g Units: grams (g) Description: <p>Total liver lipid content</p> http://lod.bco-dmo.org/id/dataset-parameter/938107.rdf Name: liver_lean_g Units: grams (g) Description: <p>Total liver lean content</p> http://lod.bco-dmo.org/id/dataset-parameter/938108.rdf Name: P_gonad_lipid Units: percent (%) Description: <p>Percent gonad lipid content (gonad_lipid_g /gonad_dW*100)</p> http://lod.bco-dmo.org/id/dataset-parameter/938109.rdf Name: P_gonad_lean Units: percent (%) Description: <p>Percent gonad lean content (gonad_lean_g/gonad_dW*100)</p> http://lod.bco-dmo.org/id/dataset-parameter/938110.rdf Name: P_liver_lipd Units: percent (%) Description: <p>Percent liver lipid content (liver_lipid_g /liver_dW*100)</p> http://lod.bco-dmo.org/id/dataset-parameter/938111.rdf Name: P_liver_lean Units: percent (%) Description: <p>Percent liver lean content (liver_lean_g/liver_dW*100)</p> http://lod.bco-dmo.org/id/dataset-parameter/938112.rdf Name: w_muscle_lipid_g Units: grams (g) Description: <p>Total white muscle lipid in subsample</p> http://lod.bco-dmo.org/id/dataset-parameter/938113.rdf Name: w_muscle_lean_g Units: grams (g) Description: <p>Total white muscle lean in subsample</p> http://lod.bco-dmo.org/id/dataset-parameter/938114.rdf Name: p_w_muscle_lip Units: percent (%) Description: <p>Percent white muscle lipid content (w_muscle_lipid_g/w_muscle_sub_dW*100)</p> http://lod.bco-dmo.org/id/dataset-parameter/938115.rdf Name: p_w_muscle_lean Units: percent (%) Description: <p>Percent white muscle lean content (w_muscle_lean_g/w_muscle_sub_dW*100)</p> http://lod.bco-dmo.org/id/dataset-parameter/938116.rdf Name: Notes Units: unitless Description: <p>Notes</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 13200 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/dba761e1-8f10-4e45-a18d-8b1fa623b5f8/content download https://doi.org/10.26008/1912/bco-dmo.938004.1 download onLine dataset &lt;p&gt;&lt;strong&gt;Experimental setup:&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;50 adult specimens were collected by boat off Stonington Borough, CT (41° 20’37.8” N 71° 54’51.4” W) between September 15th and October 10th, 2022. Individuals were collected via hook and line angling and were transported in aerated coolers to the Rankin seawater laboratory where adults were acclimated and equally split between two 3785-l tanks. Each tank was supplied with flowing, sand-filtered seawater from eastern LIS which naturally ranges in temperature from 25 to 4°C and a salinity between 28 to 33 ‰. Prior to the start of the experiment adults were fed previously frozen loligo squid (Seafreeze LLC, North Kingstown RI) 3× a week ad libitum.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Experimental design:&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;The adult experiment assessed the inshore overwintering potential of adult BSB under two distinct diets and a current inshore temperature regime for 200 days (October 10th, 2022, to April 28th, 2023). Following each specimen collection period, a subset of individuals was immediately euthanized with an overdose of MS-222 to act as a wild ‘pre-migratory’ baseline (‘fall’, n = 17) and dissected (“see methods in “Adult black sea bass winter survival and lipid dynamics: Wild fish”).&lt;/p&gt; &lt;p&gt;At the start of the experiment (October 10th, 2022) 50 specimens were briefly anesthetized, measured in length (TL = 32.8 ± 6.6 cm) and weight (wW = 514.7 ± 250), tagged with monofilament Floy-tags, and randomly allocated to one of two 3785-l tanks which was allocated a diet treatment; ‘mussels’ (Mytilus edulis; American Mussel Harvesters, North Kingston RI) and ‘herring’ (Clupea harrengus; Seafreeze ltd., North Kingston RI).&lt;/p&gt; &lt;p&gt;Fish were then fed 2% of the initial stocked biomass 3× a week throughout the experiment. Experimental tanks were flow-through (9060 l h-1), with conditions ambient to local LIS conditions and had a set of lights whose photoperiod was gradually changed weekly (11.5 h light: 12.5 h dark to 9 h light: 15 h dark and back up). Temperature was monitored in one tank by a Eureka Manta probe which recorded tank temperature every 30 minutes, while pH was monitored twice weekly via a handheld pH probe (Hach Handheld HQ40D), ammonia and salinity were measured once a week, and each tank was siphoned twice weekly to remove detritus. Over the course of the experiment there was no ammonia (0 ppm), while the mean pH and salinity were 8.04 ± 0.12 pH units and 31.8 ± 1.07 ‰, respectively.&lt;/p&gt; &lt;p&gt;Over the course of the experiment, fish were examined twice daily for mortalities or loss of equilibria. In the case of lost equilibria, specimens were immediately removed, euthanized with an overdose of MS-222, and dissected as previously described. Over the course of the experiment three individuals from the Herring diet treatment jumped overnight and weren’t included in subsequent analyses.&lt;/p&gt; &lt;p&gt;Since C. striata tend to have a high site-fidelity, beginning of April 2023, sampling occurred weekly on the same reef that the experimental and fall baseline specimens were collected on in order to collect ‘post-migratory’ baseline specimens (‘spring’, n = 21), upon their first entry into LIS (April 27th, 2023; see methods in “Adult black sea bass winter survival and lipid dynamics: Wild fish”). Specimens were immediately euthanized, measured, and dissected as previously described and the experiment ended the following day (April 28th, 2023). Upon ending the experiment all surviving specimens were measured for length (TL; 33.7 ± 4.4 cm), body depth (BD; 8 ± 0.9 cm), and wet weight (wW; 532.6 ± 227 g). During dissections, the stomach was removed to calculate a stomachless whole weight to standardize for consumed prey items, and the liver and gonad was then removed, individually weighed (0.01 g) and frozen at -20°C for future lipid extractions. A subsample of dorsally located white muscle tissue was also removed and frozen.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Response traits:&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;For experimental adults, we only calculated cumulative growth in length (GR), and specific growth rates (SGR). For all adults, we calculated gonadosomatic (GSI; %) and hepatosomatic (HSI; %) indices using stomachless fish mass (wW – stomach mass), to standardize for stomach contents, as {e.g., 100 × [(gonad or liver mass, g) / (stomachless fish mass)].&lt;/p&gt; &lt;p&gt;We quantified gonad, liver, and white muscle, storage lipid, lean-mass, and ash weights of each surviving experimental individual and baseline specimen. Samples were frozen at -50°C for 1 week and remeasured for whole body dry weight (dWb, 0.001 g). Following published protocols (Schultz and Conover 1997, Guo et al. 2021, 2022, Zavell et al. 2023), dried specimens were loaded into preweighed Alundum medium-porosity extraction thimbles and transferred to a custom-designed Soxhlet apparatus, where they were bathed in petroleum ether for 3.5 h to extract all metabolically available lipids. Samples were then dried overnight (60°C) and re-measured, with the change in pre- and post-extraction weights (ΔdW), representing the storage-lipid fraction (dWLipid). Samples were then muffle furnaced for 4 h at 550°C and reweighed with ΔdW, representing the lean-mass fraction (dWLean) and the remaining mass represents the inorganic fraction (dWAsh).&amp;nbsp;&lt;br /&gt; &lt;br /&gt; &lt;strong&gt;Organism Identifier (LSID, Life Sciences Identifier)&lt;/strong&gt;&lt;br /&gt; Centropristis striata, urn:lsid:marinespecies.org:taxname:159348&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;See methods section.&amp;nbsp;&lt;/p&gt; Specified by the Principal Investigator(s) * Sheet &quot;Experiment&quot; of submitted file &quot;Adult-BSB-Overwintering-Experiment-BCO-DMO-V4.xlsx&quot; was imported into the BCO-DMO data system as the primary data table for this dataset. Sheet &quot;Temperature&quot; of file &quot;Adult-BSB-Overwintering-Exp-BCO-DMO-V3.xlsx&quot; was imported as a supplemental table. ** Missing data values are displayed differently based on the file format you download.  They are blank in csv files, &quot;NaN&quot; in MatLab files, etc. * Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores.  Can not start with a number] * Dates converted to ISO 8601 format * Latitude and Longitude converted to decimal degree format (South and west are negative). example lon 71.9332°W to -71.9332 Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Hach Handheld pH and Temperature probe (HQ2200 Multi/2 Channel) Hach Handheld pH and Temperature probe (HQ2200 Multi/2 Channel) PI Supplied Instrument Name: Hach Handheld pH and Temperature probe (HQ2200 Multi/2 Channel) Instrument Name: Multi Parameter Portable Meter Instrument Short Name: Instrument Description: An analytical instrument that can measure multiple parameters, such as pH, EC, TDS, DO and temperature with one device and is portable or hand-held. Mettler Toledo Balance (XPR1202S) Mettler Toledo Balance (XPR1202S) PI Supplied Instrument Name: Mettler Toledo Balance (XPR1202S) Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/ PI Supplied Instrument Name: PI Supplied Instrument Description:Custom-designed Soxhlet apparatus for Lipid/Lean analyzes – UConn Storrs – self designed and assembled  Instrument Name: Soxhlet extractor Instrument Short Name: Instrument Description: A Soxhlet extractor is a piece of laboratory apparatus designed for the extraction of a lipid from a solid material. The solid is placed in a filter paper thimble which is then placed into the main chamber of the Soxhlet extractor. The solvent (heated to reflux) travels into the main chamber and the partially soluble components are slowly transferred to the solvent. HOBO Pendant MX Water Temperature Data Logger HOBO Pendant MX Water Temperature Data Logger PI Supplied Instrument Name: HOBO Pendant MX Water Temperature Data Logger Instrument Name: Temperature Logger Instrument Short Name: Instrument Description: Records temperature data over a period of time.