http://lod.bco-dmo.org/id/dataset/938012
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-09-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Adult Black Sea Bass (Centropristis striata) winter survival and lipid accumulation in wild-caught fish in Long Island Sound in Sept of 2022 to Apr of 2023
2024-09-23
publication
2024-09-23
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-09-24
publication
https://doi.org/10.26008/1912/bco-dmo.938012.1
Hannes Baumann
University of Connecticut
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Baumann, H., Zavell, M. D. (2024) Adult Black Sea Bass (Centropristis striata) winter survival and lipid accumulation in wild-caught fish in Long Island Sound in Sept of 2022 to Apr of 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-09-23 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.938012.1 [access date]
Methods and Sampling: <p>17 adult specimens were collected by boat off Stonington Borough, CT (41° 20’37.8” N 71° 54’51.4” W) between September 15th and October 10th, 2022 to act as a wild ‘pre-migratory’ baseline (‘fall’, n = 17) and an additional 21 individuals were collected from the same location on April 27<sup>th</sup> 2021 to act as a wild “post-migratory” baseline (‘spring’, n = 21). Upon collection, individuals were immediately euthanized with MS-222 and measured for length (TL; 33.7 ± 4.4 cm), body depth (BD; 8 ± 0.9 cm), and wet weight (wW; 532.6 ± 227 g). During dissections, the stomach was removed to calculate a stomachless whole weight to standardize for consumed prey items, and the liver and gonad was then removed, individually weighed (0.01 g) and frozen at -20°C for future lipid extractions. A subsample of dorsally located white muscle tissue was also removed and frozen.</p>
<p>For all adults, we calculated gonadosomatic (GSI; %) and hepatosomatic (HSI; %) indices using stomachless fish mass (wW – stomach mass), to standardize for stomach contents, as {e.g., 100 × [(gonad or liver mass, g) / (stomachless fish mass)].</p>
<p>We quantified gonad, liver, and white muscle, storage lipid, lean-mass, and ash weights of each surviving experimental individual and baseline specimen. Samples were frozen at -50°C for 1 week and remeasured for whole body dry weight (dWb, 0.001 g). Following published protocols (Schultz and Conover 1997, Guo et al. 2021, 2022, Zavell et al. 2023), dried specimens were loaded into preweighed Alundum medium-porosity extraction thimbles and transferred to a custom-designed Soxhlet apparatus, where they were bathed in petroleum ether for 3.5 h to extract all metabolically available lipids. Samples were then dried overnight (60°C) and re-measured, with the change in pre- and post-extraction weights (ΔdW), representing the storage-lipid fraction (dWLipid). Samples were then muffle furnaced for 4 h at 550°C and reweighed with ΔdW, representing the lean-mass fraction (dWLean) and the remaining mass represents the inorganic fraction (dWAsh).&nbsp;</p>
<p><strong>Organism Identifier (LSID, Life Sciences Identifier)</strong><br />
Centropristis striata, urn:lsid:marinespecies.org:taxname:159348</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1536336 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1536336
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756751 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756751
Funding provided by Connecticut Sea Grant (CTSG) Award Number: R/LR-30
completed
Hannes Baumann
University of Connecticut
860-405-9297
Department of Marine Sciences 1080 Shennecossett Road
Groton
CT
06340-6048
USA
hannes.baumann@uconn.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Species
Collection_Location
Collection_Longitude
Collection_Latitude
Collection_Date
Sampling_Date
Fish_ID
Season
TL
wW
Kwet
stomach_wW
stomachless_wW
stomach_content_wW
gonad_wW
gonad_dW
liver_wW
liver_dW
w_muscle_sub_dW
Sex
GSI
HSI
gonad_lipid_g
gonad_lean_g
liver_lipid_g
liver_lean_g
P_gonad_lipid
P_gonad_lean
P_liver_lipd
P_liver_lean
w_muscle_lipid_g
w_muscle_lean_g
p_w_muscle_lip
p_w_muscle_lean
Notes
Hach Handheld pH and Temperature probe (HQ2200 Multi/2 Channel)
Mettler Toledo Balance (XPR1202S)
HOBO Pendant MX Water Temperature Data Logger
theme
None, User defined
species scientific name (binomial)
site
longitude
latitude
date
individual
sample description
fish_len
weight
sex
lipids in biota
comments
featureType
BCO-DMO Standard Parameters
Multi Parameter Portable Meter
scale or balance
Soxhlet extractor
Temperature Logger
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative research: The genomic underpinnings of local adaptation despite gene flow along a coastal environmental cline
https://befel.marinesciences.uconn.edu/2018/03/07/research-news-new-nsf-grant-to-study-silverside-genes/
Collaborative research: The genomic underpinnings of local adaptation despite gene flow along a coastal environmental cline
<p>NSF Abstract:</p>
<p>Oceans are large, open habitats, and it was previously believed that their lack of obvious barriers to dispersal would result in extensive mixing, preventing organisms from adapting genetically to particular habitats. It has recently become clear, however, that many marine species are subdivided into multiple populations that have evolved to thrive best under contrasting local environmental conditions. Nevertheless, we still know very little about the genomic mechanisms that enable divergent adaptations in the face of ongoing intermixing. This project focuses on the Atlantic silverside (Menidia menidia), a small estuarine fish that exhibits a remarkable degree of local adaptation in growth rates and a suite of other traits tightly associated with a climatic gradient across latitudes. Decades of prior lab and field studies have made Atlantic silverside one of the marine species for which we have the best understanding of evolutionary tradeoffs among traits and drivers of selection causing adaptive divergence. Yet, the underlying genomic basis is so far completely unknown. The investigators will integrate whole genome sequencing data from wild fish sampled across the distribution range with breeding experiments in the laboratory to decipher these genomic underpinnings. This will provide one of the most comprehensive assessments of the genomic basis for local adaptation in the oceans to date, thereby generating insights that are urgently needed for better predictions about how species can respond to rapid environmental change. The project will provide interdisciplinary training for a postdoc as well as two graduate and several undergraduate students from underrepresented minorities. The findings will also be leveraged to develop engaging teaching and outreach materials (e.g. a video documentary and popular science articles) to promote a better understanding of ecology, evolution, and local adaptation among science students and the general public.</p>
<p>The goal of the project is to characterize the genomic basis and architecture underlying local adaptation in M. menidia and examine how the adaptive divergence is shaped by varying levels of gene flow and maintained over ecological time scales. The project is organized into four interconnected components. Part 1 examines fine-scale spatial patterns of genomic differentiation along the adaptive cline to a) characterize the connectivity landscape, b) identify genomic regions under divergent selection, and c) deduce potential drivers and targets of selection by examining how allele frequencies vary in relation to environmental factors and biogeographic features. Part 2 maps key locally adapted traits to the genome to dissect their underlying genomic basis. Part 3 integrates patterns of variation in the wild (part 1) and the mapping of traits under controlled conditions (part 2) to a) examine how genomic architectures underlying local adaptation vary across gene flow regimes and b) elucidating the potential role of chromosomal rearrangements and other tight linkage among adaptive alleles in facilitating adaptation. Finally, part 4 examines dispersal - selection dynamics over seasonal time scales to a) infer how selection against migrants and their offspring maintains local adaptation despite homogenizing connectivity and b) validate candidate loci for local adaptation. Varying levels of gene flow across the species range create a natural experiment for testing general predictions about the genomic mechanisms that enable adaptive divergence in the face of gene flow. The findings will therefore have broad implications and will significantly advance our understanding of the role genomic architecture plays in modifying the gene flow - selection balance within coastal environments.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
GenomAdapt
largerWorkCitation
project
eng; USA
oceans
-71.9332
-71.9332
41.3045
41.3045
2022-09-15
2023-04-27
Eastern coastline of North America
0
BCO-DMO catalogue of parameters from Adult Black Sea Bass (Centropristis striata) winter survival and lipid accumulation in wild-caught fish in Long Island Sound in Sept of 2022 to Apr of 2023
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/938037.rdf
Name: Species
Units: unitless
Description: <p>Black Sea Bass - Centropristis striata. LSID: urn:lsid:marinespecies.org:taxname:159348</p>
http://lod.bco-dmo.org/id/dataset-parameter/938038.rdf
Name: Collection_Location
Units: unitless
Description: <p>Stonington Borough, CT</p>
http://lod.bco-dmo.org/id/dataset-parameter/938039.rdf
Name: Collection_Longitude
Units: decimal degrees
Description: <p>Longitute of collection site (Stonington Borough)</p>
http://lod.bco-dmo.org/id/dataset-parameter/938040.rdf
Name: Collection_Latitude
Units: decimal degrees
Description: <p>Latitude of collection site (Stonington Borough)</p>
http://lod.bco-dmo.org/id/dataset-parameter/938041.rdf
Name: Collection_Date
Units: unitless
Description: <p>Date of fish collection in the wild</p>
http://lod.bco-dmo.org/id/dataset-parameter/938042.rdf
Name: Sampling_Date
Units: unitless
Description: <p>Date of fish sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/938043.rdf
Name: Fish_ID
Units: unitless
Description: <p>ID of each individual fish</p>
http://lod.bco-dmo.org/id/dataset-parameter/938044.rdf
Name: Season
Units: unitless
Description: <p>Sampling Season (fall or spring)</p>
http://lod.bco-dmo.org/id/dataset-parameter/938045.rdf
Name: TL
Units: centimenter (cm)
Description: <p>Total length at sampling date</p>
http://lod.bco-dmo.org/id/dataset-parameter/938046.rdf
Name: wW
Units: grams (g)
Description: <p>Whole body wet weight at sampling date</p>
http://lod.bco-dmo.org/id/dataset-parameter/938047.rdf
Name: Kwet
Units: grams per cubic centimeter(g/cm3)
Description: <p>Fulton's condition index using wet weight at sampling date. Fulton’s Condition Index is: (Weight / TL^3)*100 (see Fulton, TW (1902); Ricker, WE (1975)).</p>
http://lod.bco-dmo.org/id/dataset-parameter/938048.rdf
Name: stomach_wW
Units: grams (g)
Description: <p>Stomach weight at sampling date</p>
http://lod.bco-dmo.org/id/dataset-parameter/938049.rdf
Name: stomachless_wW
Units: grams (g)
Description: <p>Stomachless weight at tat experiment end (wWF - Stomach.wW)</p>
http://lod.bco-dmo.org/id/dataset-parameter/938050.rdf
Name: stomach_content_wW
Units: grams (g)
Description: <p>Weight of stomach contants</p>
http://lod.bco-dmo.org/id/dataset-parameter/938051.rdf
Name: gonad_wW
Units: grams (g)
Description: <p>Gonad wet weight at sampling date</p>
http://lod.bco-dmo.org/id/dataset-parameter/938052.rdf
Name: gonad_dW
Units: grams (g)
Description: <p>Gonad dry weight at sampling date</p>
http://lod.bco-dmo.org/id/dataset-parameter/938053.rdf
Name: liver_wW
Units: grams (g)
Description: <p>Liver wet weight at sampling date</p>
http://lod.bco-dmo.org/id/dataset-parameter/938054.rdf
Name: liver_dW
Units: grams (g)
Description: <p>Liver dry weight at sampling date</p>
http://lod.bco-dmo.org/id/dataset-parameter/938055.rdf
Name: w_muscle_sub_dW
Units: grams (g)
Description: <p>Dry weight of the subsample of white muscle used to run lipid and lean analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/938056.rdf
Name: Sex
Units: unitless
Description: <p>Sex via visual observation of the gonads (M = male, F = female, U = unknown)</p>
http://lod.bco-dmo.org/id/dataset-parameter/938057.rdf
Name: GSI
Units: percent (%)
Description: <p>Gonadosomatic Index (gonad_wW / wWF *100)</p>
http://lod.bco-dmo.org/id/dataset-parameter/938058.rdf
Name: HSI
Units: percent (%)
Description: <p>Hepatosomatic Index (liver_wW / wWF *100)</p>
http://lod.bco-dmo.org/id/dataset-parameter/938059.rdf
Name: gonad_lipid_g
Units: grams (g)
Description: <p>Total gonad lipid content</p>
http://lod.bco-dmo.org/id/dataset-parameter/938060.rdf
Name: gonad_lean_g
Units: grams (g)
Description: <p>Total gonad lean content</p>
http://lod.bco-dmo.org/id/dataset-parameter/938061.rdf
Name: liver_lipid_g
Units: grams (g)
Description: <p>Total liver lipid content</p>
http://lod.bco-dmo.org/id/dataset-parameter/938062.rdf
Name: liver_lean_g
Units: grams (g)
Description: <p>Total liver lean content</p>
http://lod.bco-dmo.org/id/dataset-parameter/938063.rdf
Name: P_gonad_lipid
Units: percent (%)
Description: <p>Percent gonad lipid content (gonad_lipid_g /gonad_dW*100)</p>
http://lod.bco-dmo.org/id/dataset-parameter/938064.rdf
Name: P_gonad_lean
Units: percent (%)
Description: <p>Percent gonad lean content (gonad_lean_g/gonad_dW*100)</p>
http://lod.bco-dmo.org/id/dataset-parameter/938065.rdf
Name: P_liver_lipd
Units: percent (%)
Description: <p>Percent liver lipid content (liver_lipid_g /liver_dW*100)</p>
http://lod.bco-dmo.org/id/dataset-parameter/938066.rdf
Name: P_liver_lean
Units: percent (%)
Description: <p>Percent liver lean content (liver_lean_g/liver_dW*100)</p>
http://lod.bco-dmo.org/id/dataset-parameter/938067.rdf
Name: w_muscle_lipid_g
Units: grams (g)
Description: <p>Total white muscle lipid in subsample</p>
http://lod.bco-dmo.org/id/dataset-parameter/938068.rdf
Name: w_muscle_lean_g
Units: grams (g)
Description: <p>Total white muscle lean in subsample</p>
http://lod.bco-dmo.org/id/dataset-parameter/938069.rdf
Name: p_w_muscle_lip
Units: percent (%)
Description: <p>Percent white muscle lipid content (w_muscle_lipid_g/w_muscle_sub_dW*100)</p>
http://lod.bco-dmo.org/id/dataset-parameter/938070.rdf
Name: p_w_muscle_lean
Units: percent (%)
Description: <p>Percent white muscle lean content (w_muscle_lean_g/w_muscle_sub_dW*100)</p>
http://lod.bco-dmo.org/id/dataset-parameter/938071.rdf
Name: Notes
Units: unitless
Description: <p>Notes</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
12489
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/bd8d5e5e-7b92-4443-9381-9f4f58d06149/content
download
https://doi.org/10.26008/1912/bco-dmo.938012.1
download
onLine
dataset
<p>17 adult specimens were collected by boat off Stonington Borough, CT (41° 20’37.8” N 71° 54’51.4” W) between September 15th and October 10th, 2022 to act as a wild ‘pre-migratory’ baseline (‘fall’, n = 17) and an additional 21 individuals were collected from the same location on April 27<sup>th</sup> 2021 to act as a wild “post-migratory” baseline (‘spring’, n = 21). Upon collection, individuals were immediately euthanized with MS-222 and measured for length (TL; 33.7 ± 4.4 cm), body depth (BD; 8 ± 0.9 cm), and wet weight (wW; 532.6 ± 227 g). During dissections, the stomach was removed to calculate a stomachless whole weight to standardize for consumed prey items, and the liver and gonad was then removed, individually weighed (0.01 g) and frozen at -20°C for future lipid extractions. A subsample of dorsally located white muscle tissue was also removed and frozen.</p>
<p>For all adults, we calculated gonadosomatic (GSI; %) and hepatosomatic (HSI; %) indices using stomachless fish mass (wW – stomach mass), to standardize for stomach contents, as {e.g., 100 × [(gonad or liver mass, g) / (stomachless fish mass)].</p>
<p>We quantified gonad, liver, and white muscle, storage lipid, lean-mass, and ash weights of each surviving experimental individual and baseline specimen. Samples were frozen at -50°C for 1 week and remeasured for whole body dry weight (dWb, 0.001 g). Following published protocols (Schultz and Conover 1997, Guo et al. 2021, 2022, Zavell et al. 2023), dried specimens were loaded into preweighed Alundum medium-porosity extraction thimbles and transferred to a custom-designed Soxhlet apparatus, where they were bathed in petroleum ether for 3.5 h to extract all metabolically available lipids. Samples were then dried overnight (60°C) and re-measured, with the change in pre- and post-extraction weights (ΔdW), representing the storage-lipid fraction (dWLipid). Samples were then muffle furnaced for 4 h at 550°C and reweighed with ΔdW, representing the lean-mass fraction (dWLean) and the remaining mass represents the inorganic fraction (dWAsh).&nbsp;</p>
<p><strong>Organism Identifier (LSID, Life Sciences Identifier)</strong><br />
Centropristis striata, urn:lsid:marinespecies.org:taxname:159348</p>
Specified by the Principal Investigator(s)
<p>See methods section.&nbsp;</p>
Specified by the Principal Investigator(s)
* Sheet "Wild Fish" of submitted file "Adult-BSB-Overwintering-Wild-Fish-BCO-DMO-V4.xlsx" was imported into the BCO-DMO data system as the primary data table for this dataset.
** Missing data values are displayed differently based on the file format you download. They are blank in csv files, "NaN" in MatLab files, etc.
* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores. Can not start with a number]
* Dates converted to ISO 8601 format
* Latitude and Longitude converted to decimal degree format (South and west are negative). example lon 71.9332°W to -71.9332
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Hach Handheld pH and Temperature probe (HQ2200 Multi/2 Channel)
Hach Handheld pH and Temperature probe (HQ2200 Multi/2 Channel)
PI Supplied Instrument Name: Hach Handheld pH and Temperature probe (HQ2200 Multi/2 Channel) Instrument Name: Multi Parameter Portable Meter Instrument Short Name: Instrument Description: An analytical instrument that can measure multiple parameters, such as pH, EC, TDS, DO and temperature with one device and is portable or hand-held.
Mettler Toledo Balance (XPR1202S)
Mettler Toledo Balance (XPR1202S)
PI Supplied Instrument Name: Mettler Toledo Balance (XPR1202S) Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/
PI Supplied Instrument Name: PI Supplied Instrument Description:Custom-designed Soxhlet apparatus for Lipid/Lean analyzes – UConn Storrs – self designed and assembled Instrument Name: Soxhlet extractor Instrument Short Name: Instrument Description: A Soxhlet extractor is a piece of laboratory apparatus designed for the extraction of a lipid from a solid material. The solid is placed in a filter paper thimble which is then placed into the main chamber of the Soxhlet extractor. The solvent (heated to reflux) travels into the main chamber and the partially soluble components are slowly transferred to the solvent.
HOBO Pendant MX Water Temperature Data Logger
HOBO Pendant MX Water Temperature Data Logger
PI Supplied Instrument Name: HOBO Pendant MX Water Temperature Data Logger Instrument Name: Temperature Logger Instrument Short Name: Instrument Description: Records temperature data over a period of time.