http://lod.bco-dmo.org/id/dataset/939782
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-10-08
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Oxygen consumption by lobster larvae at different temperatures using closed cell respirometry for lobster larvae from Gulf of Maine near Boothbay Maine from 2021 to 2023 (Lobster Thermal Thresholds project)
2025-03-19
publication
2025-03-19
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-05-13
publication
https://doi.org/10.26008/1912/bco-dmo.939782.1
Eric R. Annis
Hood College
principalInvestigator
Markus Frederich
University of New England - Marine Science Center
principalInvestigator
Douglas B. Rasher
Bigelow Laboratory for Ocean Sciences
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Annis, E. R., Frederich, M., Rasher, D. B. (2025) Oxygen consumption by lobster larvae at different temperatures using closed cell respirometry for lobster larvae from Gulf of Maine near Boothbay Maine from 2021 to 2023 (Lobster Thermal Thresholds project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-03-19 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.939782.1 [access date]
Dataset Description: <p>The temporal bounds of this dataset represent the experimental trial dates (not the specimen hatch or capture dates).</p> Methods and Sampling: <p>Microrespirometry trials were conducted on planktonic larval stages I, II, III, IV, and benthic stage V <em>Homarus americanus</em>&nbsp;(lsid:marinespecies.org:taxname:156134) larvae at temperatures ranging from 4-32°C. We used a Unisense MicroRespiration System, with SensorTrace Rate software and Oxygen MicroOptode MR optical oxygen sensors.&nbsp;Sensors were calibrated at 0 and 100% oxygen saturation at each treatment temperature prior to experimental trials. Larvae were&nbsp;held at 18°C until the time of the experiment when they were transferred to a 20 ml vial and placed in a water bath at the treatment temperature for a 10-minute acclimation period.&nbsp;Acclimated larvae were transferred to respiration chambers filled with 0.45&nbsp;µm filtered seawater at treatment temperature and lowered into the water bath for a 20-minute respirometry trial.&nbsp;4 ml respiration chambers were used for stages I-III and 40 ml for stages IV and V.&nbsp;Only one larva was used in each chamber.&nbsp;Magnetic stir bars were used in all trials.&nbsp;In trials for resting oxygen consumption rate, a stainless steel mesh separated the larva from the stir bar.&nbsp;In the actively swimming trials, the mesh was removed and movement of the stir bar prevented the larva from resting on the bottom.&nbsp; Controls were run using respiration chambers with 45&nbsp;µm filtered sea water and no larva. The first 10 minutes of data collected during the trial were discarded as it often required time to reach a linear rate of oxygen consumption.&nbsp;The rate of oxygen consumption was determined for approximately the last 10 minutes of the trial using Unisense SensorTrace Rate software.&nbsp;</p>
<p>After the trial was completed the larvae were rinsed with DI water, frozen individually, and subsequently dried at 60°C for 24 hours to obtain dry weight of individuals.&nbsp; When dry weight for an individual was not available, and average values for the season was used. Oxygen consumption rate as normalized to dry weight of the individual larva.</p>
<p>Larvae were reared in the lab under several different conditions.&nbsp;Most larvae were reared individually in an environmental control room at 18°C in 400 ml glass jars in 0.45 µm filtered seawater and fed freshly hatched brine shrimp ad libitum.&nbsp;Water changes were made every 2-3 days.&nbsp;Alternative rearing conditions included 14°C and fed fresh hatched brine shrimp, ambient seawater temperature (jars were held in a water bath of flow-through seawater)&nbsp;fed fresh hatched brine shrimp, and 18°C and fed a diet of live freshly collected zooplankton from local waters.&nbsp; We also conducted&nbsp;trials on wild-caught stage IV larvae.&nbsp;Larvae were collected using a neuston net (0-0.5 m depth) in the vicinity of Boothbay, Maine, USA.&nbsp;Wild larvae were held in individual jars at ambient seawater temperature until trials could be conducted.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1948146 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1948146
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1947639 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1947639
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1948108 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1948108
completed
Eric R. Annis
Hood College
301 740 5646 (cell)
Department of Biology 401 Rosemont Ave
Frederick
MD
21701
annis@hood.edu
pointOfContact
Markus Frederich
University of New England - Marine Science Center
2076022406
11 Hills Beach Road
Biddeford
ME
040055209
US
mfrederich@une.edu
pointOfContact
Douglas B. Rasher
Bigelow Laboratory for Ocean Sciences
207-315-2567
60 Bigelow Drive
East Boothbay
ME
04544
USA
drasher@bigelow.org
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Larva_ID
Treatment_Temperature
Diet
Rearing_Temperature
Developmental_Stage
Individual_Larva_Number
Year
Hatch_or_Capture_Date
Trial_Date
Trial_Time_Local_EST
Trial_Datetime_UTC
Treatment
O2_Slope
Chamber_Number
Chamber_Volume
Treatment_O2_Consumption
Control_O2_Consumption
Control_Corrected
Dry_Weights
O2_Consumption_Normalized
Unisense MicroRespiration System
theme
None, User defined
experiment id
treatment
stage
individual
year
date
time of day
ISO_DateTime_UTC
O2 consumption
object ID
volume
weight
featureType
BCO-DMO Standard Parameters
Respirometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RUI: Collaborative Research: Linking physiological thermal thresholds to the distribution of lobster settlers and juveniles
https://osprey.bco-dmo.org/project/845227
RUI: Collaborative Research: Linking physiological thermal thresholds to the distribution of lobster settlers and juveniles
<p><em>NSF Award Abstract:</em><br />
Temperature is one critical factor that determines the distribution of marine organisms. However, in many cases temperature ranges (thermal tolerances) are only known for adults, but not for the immature stages that transition from the plankton to the bottom. This study is testing how temperature affects where larvae are settling. The American lobster (Homarus americanus) in the Gulf of Maine is serving as a model system to measure the thermal tolerance of the larvae and link this to the distribution of young lobsters in the field. Presently, lobster larvae are more likely to experience relatively cold temperatures than heat stress and larval settlement appears to be restricted to warmer shallow waters by a sensitivity to temperatures below 12°C. As water temperature has increased, settlement and juvenile distribution have expanded into deeper waters suggesting a release from cold stress. This project is advancing the understanding of shifting species distributions in response to increasing ocean temperatures by exploring thermal sensitivity in wild-caught larvae for the first time. This information is providing thermal thresholds for modeling larval viability in response to climate change scenarios. Understanding the larvae?s responses to temperature is fundamental to predicting the impact of climate change on one of the most valuable commercial fisheries in North America. The project is supporting training of undergraduate interns and a master?s student from small colleges (Hood College and University of New England) and connecting them with a research institution (Bigelow Laboratory for Ocean Sciences). Teacher training is occurring in collaboration with the Marine Science Center at the University of New England. Results from this study are being shared with stakeholders and contributing to science-based management of the lobster fishery.</p>
<p>This project is the first to examine how thermal stress on a larval stage determines juvenile distributions using a combination of correlative and experimental approaches that includes measuring biochemical stress indicators in larvae deployed in natural field habitats. The central hypothesis is that the physiology of individual planktonic larvae controls meso-scale settlement patterns in the field. The goal is to ascertain if there is a causal relationship between the underlying physiology and thermal sensitivity of the organism and the distribution of early life stages. Larval supply, settlement and juvenile abundances will be assessed at different depths with temperatures above and below the proposed minimum temperature threshold of 12°C for larvae. Laboratory experiments using conventional methods are determining thermal tolerances in wild-caught larvae and how they change with ontogeny. The upper and lower thermal optima are being resolved using multiple physiological parameters such as measurements of oxygen consumption and aerobic scope, and biochemical assays of thermal stress (HSP70, AMPK, and SIRT). To link physiology to settlement patterns, caged stage IV larvae and V juveniles are being deployed in the field at sites with temperatures above and below 12°C. Lethal and sub-lethal effects on caged lobsters are being evaluated through measures of growth, mortality and biochemical markers of thermal stress. This is the first study to focus on the thermal tolerance of wild larvae, which has broad implications for understanding settling in marine invertebrate larvae.</p>
Lobster Thermal Thresholds
largerWorkCitation
project
eng; USA
oceans
-69.7615
-69.5025
43.6558
43.8453
2021-06-14
2023-07-18
Gulf of Maine
0
BCO-DMO catalogue of parameters from Oxygen consumption by lobster larvae at different temperatures using closed cell respirometry for lobster larvae from Gulf of Maine near Boothbay Maine from 2021 to 2023 (Lobster Thermal Thresholds project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/956652.rdf
Name: Larva_ID
Units: unitless
Description: <p>Larva ID consists of the following terms from left to right: Treatment temperature; Rearing temperature (14 degrees C, 18 degrees C, Ambient, Wild); Developmental Stage (1, 2, 3, 4, 5); Experiment (Respirometry, Growth, Molecular); Sequential Number (1, 2, 3...n OR CTRL Testing or CTRL Active).</p>
http://lod.bco-dmo.org/id/dataset-parameter/956653.rdf
Name: Treatment_Temperature
Units: degrees C
Description: <p>Temperature of experimental trial.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956654.rdf
Name: Diet
Units: unitless
Description: <p>Diet larvae were fed during rearing. Z = zooplankton, B = brine shrimp.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956655.rdf
Name: Rearing_Temperature
Units: degrees C
Description: <p>Rearing temperature; 14 = 14°C, 18 = 18°C, A = Ambient , W = Wild Caught.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956656.rdf
Name: Developmental_Stage
Units: unitless
Description: <p>Developmental stage 1 through 5.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956657.rdf
Name: Individual_Larva_Number
Units: unitless
Description: <p>Sequential numbers assigned to individuals in each trial.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956658.rdf
Name: Year
Units: unitless
Description: <p>Year in which the trial was conducted (2021, 2022, 2023).</p>
http://lod.bco-dmo.org/id/dataset-parameter/956659.rdf
Name: Hatch_or_Capture_Date
Units: unitless
Description: <p>Date that larva was hatched for lab reared larvae or caught for wild larvae.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956660.rdf
Name: Trial_Date
Units: unitless
Description: <p>Date on which the trial was conducted.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956661.rdf
Name: Trial_Time_Local_EST
Units: unitless
Description: <p>Time at which the trial was conducted in local time, EST (UTC-4).</p>
http://lod.bco-dmo.org/id/dataset-parameter/956662.rdf
Name: Trial_Datetime_UTC
Units: unitless
Description: <p>Datetime at which the trial was conducted in UTC time.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956663.rdf
Name: Treatment
Units: unitless
Description: <p>Experimental treatment during trials; Control Resting = 0.1, Control Active Swimming = 0.2, Resting = 1, Active Swimming = 2.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956664.rdf
Name: O2_Slope
Units: micromole per liter per hour (umol/l/h)
Description: <p>Rate of oxygen consumption (µmol/l/h) derived from SensorTrace Rate software.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956665.rdf
Name: Chamber_Number
Units: unitless
Description: <p>Identifier to determine the correct chamber volume to use in calculations. The leading number indicates the approximate volume (4 or 40 ml) and the number after the decimal is a sequential number assigned to individual chambers.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956666.rdf
Name: Chamber_Volume
Units: liters (l)
Description: <p>Measured volume of the respirometry chamber in liters. </p>
http://lod.bco-dmo.org/id/dataset-parameter/956667.rdf
Name: Treatment_O2_Consumption
Units: micromole per hour (umol/h)
Description: <p>Treatment O2 Consumption as represented by: [O2 Slope]*[Chamber Volume] . </p>
http://lod.bco-dmo.org/id/dataset-parameter/956668.rdf
Name: Control_O2_Consumption
Units: micromole per hour (umol/h)
Description: <p>Mean of all [O2 Slope]*[Chamber Volume] for control trials for a given temperature and year.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956669.rdf
Name: Control_Corrected
Units: micromole per larva per hour (umol/larva/h)
Description: <p>Control corrected represented by: [Treatment O2 Consumption] - [Control O2 Consumption].</p>
http://lod.bco-dmo.org/id/dataset-parameter/956670.rdf
Name: Dry_Weights
Units: milligram (mg)
Description: <p>Individual dry weight in milligrams (60°C for 24 h).</p>
http://lod.bco-dmo.org/id/dataset-parameter/956671.rdf
Name: O2_Consumption_Normalized
Units: micromole per milligram of larva per hour (umol/mg larva/hr
Description: <p>Oxygen consumption normalized to the dry weight of the larva. Normalized O2 consumption represented by: [Control Corrected]/[Dry Weight]. </p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
131372
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/ef34e608-ff16-4bac-abd4-0480b5edd247/content
download
https://doi.org/10.26008/1912/bco-dmo.939782.1
download
onLine
dataset
<p>Microrespirometry trials were conducted on planktonic larval stages I, II, III, IV, and benthic stage V <em>Homarus americanus</em>&nbsp;(lsid:marinespecies.org:taxname:156134) larvae at temperatures ranging from 4-32°C. We used a Unisense MicroRespiration System, with SensorTrace Rate software and Oxygen MicroOptode MR optical oxygen sensors.&nbsp;Sensors were calibrated at 0 and 100% oxygen saturation at each treatment temperature prior to experimental trials. Larvae were&nbsp;held at 18°C until the time of the experiment when they were transferred to a 20 ml vial and placed in a water bath at the treatment temperature for a 10-minute acclimation period.&nbsp;Acclimated larvae were transferred to respiration chambers filled with 0.45&nbsp;µm filtered seawater at treatment temperature and lowered into the water bath for a 20-minute respirometry trial.&nbsp;4 ml respiration chambers were used for stages I-III and 40 ml for stages IV and V.&nbsp;Only one larva was used in each chamber.&nbsp;Magnetic stir bars were used in all trials.&nbsp;In trials for resting oxygen consumption rate, a stainless steel mesh separated the larva from the stir bar.&nbsp;In the actively swimming trials, the mesh was removed and movement of the stir bar prevented the larva from resting on the bottom.&nbsp; Controls were run using respiration chambers with 45&nbsp;µm filtered sea water and no larva. The first 10 minutes of data collected during the trial were discarded as it often required time to reach a linear rate of oxygen consumption.&nbsp;The rate of oxygen consumption was determined for approximately the last 10 minutes of the trial using Unisense SensorTrace Rate software.&nbsp;</p>
<p>After the trial was completed the larvae were rinsed with DI water, frozen individually, and subsequently dried at 60°C for 24 hours to obtain dry weight of individuals.&nbsp; When dry weight for an individual was not available, and average values for the season was used. Oxygen consumption rate as normalized to dry weight of the individual larva.</p>
<p>Larvae were reared in the lab under several different conditions.&nbsp;Most larvae were reared individually in an environmental control room at 18°C in 400 ml glass jars in 0.45 µm filtered seawater and fed freshly hatched brine shrimp ad libitum.&nbsp;Water changes were made every 2-3 days.&nbsp;Alternative rearing conditions included 14°C and fed fresh hatched brine shrimp, ambient seawater temperature (jars were held in a water bath of flow-through seawater)&nbsp;fed fresh hatched brine shrimp, and 18°C and fed a diet of live freshly collected zooplankton from local waters.&nbsp; We also conducted&nbsp;trials on wild-caught stage IV larvae.&nbsp;Larvae were collected using a neuston net (0-0.5 m depth) in the vicinity of Boothbay, Maine, USA.&nbsp;Wild larvae were held in individual jars at ambient seawater temperature until trials could be conducted.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>SensorTrace Rate software (ver. 1.13) was used to manually select the portion of the oxygen consumption curve used to determine the rate.&nbsp; The slope of the line was recorded and transferred to an Excel spreadsheet.&nbsp; The rate (µmol/L/h) was multiplied by the chamber volume to determine the oxygen consumed per hour.&nbsp; The oxygen consumption rate in the controls were subtracted from the treatment rates.&nbsp; The resulting rate was then divided by the dry weight of the larva.&nbsp; All available control values were averaged for the season at each treatment temperature to provide more consistent values.&nbsp;&nbsp;</p>
Specified by the Principal Investigator(s)
- Units have been removed from column names.
- Blank spaces in column names have been replaced with underscores ("_").
- Added column "Trial_Datetime_UTC" from columns "Trial_Date" and "Trial_Time_Local_EST".
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Unisense MicroRespiration System
Unisense MicroRespiration System
PI Supplied Instrument Name: Unisense MicroRespiration System PI Supplied Instrument Description:A Unisense MicroRespiration System, with SensorTrace Rate software and Oxygen MicroOptode MR optical oxygen sensors, was used during microrespirometry trials on planktonic larvae. Instrument Name: Respirometer Instrument Short Name: Instrument Description: A device that measures the rate of respiration by a living organism or organic system by measuring its rate of exchange of oxygen and/or carbon dioxide.