http://lod.bco-dmo.org/id/dataset/939825
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-10-08
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Growth and mortality of lobster larvae at different temperatures for lobster larvae from Gulf of Maine near Boothbay Maine from 2021 and 2022 (Lobster Thermal Thresholds project)
2025-03-21
publication
2025-03-21
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-05-13
publication
https://doi.org/10.26008/1912/bco-dmo.939825.1
Eric R. Annis
Hood College
principalInvestigator
Markus Frederich
University of New England - Marine Science Center
principalInvestigator
Douglas B. Rasher
Bigelow Laboratory for Ocean Sciences
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Annis, E. R., Frederich, M., Rasher, D. B. (2025) Growth and mortality of lobster larvae at different temperatures for lobster larvae from Gulf of Maine near Boothbay Maine from 2021 and 2022 (Lobster Thermal Thresholds project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-03-21 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.939825.1 [access date]
Methods and Sampling: <p><em>Homarus americanus</em>&nbsp;(lsid:marinespecies.org:taxname:156134) lobster larvae were reared to the appropriate stage in lab under several different conditions.&nbsp;Most larvae were reared individually in an environmental control room at 18°C in 400 ml glass jars in 0.45 µm filtered seawater and fed fresh hatched brine shrimp ad libitum.&nbsp;Water changes were made every 2-3 days.&nbsp;Alternative rearing conditions included 14°C and fed fresh hatched brine shrimp, ambient seawater temperature (jars were held in a water bath of flow through seawater)&nbsp;fed fresh hatched brine shrimp, and 18°C and fed a diet of live, freshly collected zooplankton from local waters.&nbsp;We also conducted&nbsp;trials on wild caught stage IV larvae.&nbsp;Larvae were collected using a neuston net (0-0.5 m depth) in the vicinity of Boothbay, Maine, USA.&nbsp;Wild larvae were held in individual jars at ambient seawater temperature until trials could be conducted.&nbsp;</p>
<p>&nbsp;</p>
<p>To assess growth and mortality, larvae were transferred from their rearing condition to the treatment temperature and maintained at the treatment temperature until they either molted to the next developmental stage or died.&nbsp;Larvae were transferred in their jar at the rearing temperature to the treatment temperature without exchanging the water, which allowed for a gradual temperature change.&nbsp;Feeding and water changes continued as described for rearing conditions. When larvae molted to the next developmental stage, they were photographed for carapace length measurements and frozen individually.&nbsp;Measurements of carapace length&nbsp;were made from the posterior edge of the ocular cavity to the midline of the posterior edge of the carapace using ImageJ. Frozen larvae were subsequently dried for 24 hours at 60 °C and weighed.&nbsp; Larvae dying during the experiment were not measured or weighed.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1948146 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1948146
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1947639 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1947639
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1948108 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1948108
completed
Eric R. Annis
Hood College
301 740 5646 (cell)
Department of Biology 401 Rosemont Ave
Frederick
MD
21701
annis@hood.edu
pointOfContact
Markus Frederich
University of New England - Marine Science Center
2076022406
11 Hills Beach Road
Biddeford
ME
040055209
US
mfrederich@une.edu
pointOfContact
Douglas B. Rasher
Bigelow Laboratory for Ocean Sciences
207-315-2567
60 Bigelow Drive
East Boothbay
ME
04544
USA
drasher@bigelow.org
pointOfContact
asNeeded
Dataset Version: 1
Unknown
LARVA_ID
TREATMENT_TEMPERATURE
DIET
REARING_CONDITION
REARING_TEMPERATURE
DEVELOPMENTAL_STAGE
INDIVIDUAL_LARVA_NUMBER
HATCH_OR_CAPTURE_DATE
TRANSFER_DATE
MOLT_DATE
TIME_UNTIL_MOLT
DEATH_DATE
TIME_UNTIL_DEATH
CARAPACE_LENGTH
DRY_WEIGHT
NOTES
Source_File
Nikon SMZ745T
theme
None, User defined
experiment id
treatment
stage
individual
date
days
length
weight
comments
file_name
featureType
BCO-DMO Standard Parameters
Camera
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RUI: Collaborative Research: Linking physiological thermal thresholds to the distribution of lobster settlers and juveniles
https://osprey.bco-dmo.org/project/845227
RUI: Collaborative Research: Linking physiological thermal thresholds to the distribution of lobster settlers and juveniles
<p><em>NSF Award Abstract:</em><br />
Temperature is one critical factor that determines the distribution of marine organisms. However, in many cases temperature ranges (thermal tolerances) are only known for adults, but not for the immature stages that transition from the plankton to the bottom. This study is testing how temperature affects where larvae are settling. The American lobster (Homarus americanus) in the Gulf of Maine is serving as a model system to measure the thermal tolerance of the larvae and link this to the distribution of young lobsters in the field. Presently, lobster larvae are more likely to experience relatively cold temperatures than heat stress and larval settlement appears to be restricted to warmer shallow waters by a sensitivity to temperatures below 12°C. As water temperature has increased, settlement and juvenile distribution have expanded into deeper waters suggesting a release from cold stress. This project is advancing the understanding of shifting species distributions in response to increasing ocean temperatures by exploring thermal sensitivity in wild-caught larvae for the first time. This information is providing thermal thresholds for modeling larval viability in response to climate change scenarios. Understanding the larvae?s responses to temperature is fundamental to predicting the impact of climate change on one of the most valuable commercial fisheries in North America. The project is supporting training of undergraduate interns and a master?s student from small colleges (Hood College and University of New England) and connecting them with a research institution (Bigelow Laboratory for Ocean Sciences). Teacher training is occurring in collaboration with the Marine Science Center at the University of New England. Results from this study are being shared with stakeholders and contributing to science-based management of the lobster fishery.</p>
<p>This project is the first to examine how thermal stress on a larval stage determines juvenile distributions using a combination of correlative and experimental approaches that includes measuring biochemical stress indicators in larvae deployed in natural field habitats. The central hypothesis is that the physiology of individual planktonic larvae controls meso-scale settlement patterns in the field. The goal is to ascertain if there is a causal relationship between the underlying physiology and thermal sensitivity of the organism and the distribution of early life stages. Larval supply, settlement and juvenile abundances will be assessed at different depths with temperatures above and below the proposed minimum temperature threshold of 12°C for larvae. Laboratory experiments using conventional methods are determining thermal tolerances in wild-caught larvae and how they change with ontogeny. The upper and lower thermal optima are being resolved using multiple physiological parameters such as measurements of oxygen consumption and aerobic scope, and biochemical assays of thermal stress (HSP70, AMPK, and SIRT). To link physiology to settlement patterns, caged stage IV larvae and V juveniles are being deployed in the field at sites with temperatures above and below 12°C. Lethal and sub-lethal effects on caged lobsters are being evaluated through measures of growth, mortality and biochemical markers of thermal stress. This is the first study to focus on the thermal tolerance of wild larvae, which has broad implications for understanding settling in marine invertebrate larvae.</p>
Lobster Thermal Thresholds
largerWorkCitation
project
eng; USA
oceans
2021-06-01
2022-12-31
Gulf of Maine
0
BCO-DMO catalogue of parameters from Growth and mortality of lobster larvae at different temperatures for lobster larvae from Gulf of Maine near Boothbay Maine from 2021 and 2022 (Lobster Thermal Thresholds project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/956713.rdf
Name: LARVA_ID
Units: unitless
Description: <p>Larva ID consists of the following terms from left to right: Treatment temperature; Rearing temperature (14 degrees C, 18 degrees C, Ambient, Wild); Developmental Stage (1, 2, 3, 4, 5); Experiment (Respirometry, Growth, Molecular); Sequential Number (1, 2, 3...n OR CTRL Testing or CTRL Active).</p>
http://lod.bco-dmo.org/id/dataset-parameter/956714.rdf
Name: TREATMENT_TEMPERATURE
Units: degrees C
Description: <p>Temperature of experimental trial in °C.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956715.rdf
Name: DIET
Units: unitless
Description: <p>Diet larvae were fed during rearing. Z = zooplankton, B = brine shrimp.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956716.rdf
Name: REARING_CONDITION
Units: unitless
Description: <p>Rearing conditions; L= lab, w = wild.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956717.rdf
Name: REARING_TEMPERATURE
Units: degrees C
Description: <p>Rearing temperature; 14 = 14°C, 18 = 18°C, A = Ambient , W = Wild Caught</p>
http://lod.bco-dmo.org/id/dataset-parameter/956718.rdf
Name: DEVELOPMENTAL_STAGE
Units: unitless
Description: <p>The developmental stage at which the larva was placed in the temperature treatment (stage 1-5).</p>
http://lod.bco-dmo.org/id/dataset-parameter/956719.rdf
Name: INDIVIDUAL_LARVA_NUMBER
Units: unitless
Description: <p>Sequential numbers assigned to individuals in each treatment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956720.rdf
Name: HATCH_OR_CAPTURE_DATE
Units: unitless
Description: <p>Date that larva was hatched for lab reared larvae or caught for wild larvae.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956721.rdf
Name: TRANSFER_DATE
Units: unitless
Description: <p>Date that the larva was transferred to the temperature treatment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956722.rdf
Name: MOLT_DATE
Units: unitless
Description: <p>Date that the larva molted to the next developmental stage (only recorded if it molted).</p>
http://lod.bco-dmo.org/id/dataset-parameter/956723.rdf
Name: TIME_UNTIL_MOLT
Units: unitless
Description: <p>Number of days from transfer date to molting to the next developmental stage (only recorded if it molted). </p>
http://lod.bco-dmo.org/id/dataset-parameter/956724.rdf
Name: DEATH_DATE
Units: unitless
Description: <p>Date that the larva died (only recorded if the larva died).</p>
http://lod.bco-dmo.org/id/dataset-parameter/956725.rdf
Name: TIME_UNTIL_DEATH
Units: days
Description: <p>Number of days from transfer to death (only recorded if the larva died).</p>
http://lod.bco-dmo.org/id/dataset-parameter/956726.rdf
Name: CARAPACE_LENGTH
Units: millimeters (mm_
Description: <p>Measured in mm from the posterior edge of the ocular cavity too the the midline of the posterior edge of the carapace.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956727.rdf
Name: DRY_WEIGHT
Units: grams (g)
Description: <p>Measured in g after the larvae were died for 24 hours at 60°C.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956728.rdf
Name: NOTES
Units: unitless
Description: <p>Additional comments on individual larvae </p>
http://lod.bco-dmo.org/id/dataset-parameter/956729.rdf
Name: Source_File
Units: unitless
Description: <p>Original file name from which the corresponding row of data was sourced. Originally, the 2021 and 2022 data were stored in two separate files merged to create the primary data file published through BCO-DMO.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
-1
download
https://doi.org/10.26008/1912/bco-dmo.939825.1
download
onLine
dataset
<p><em>Homarus americanus</em>&nbsp;(lsid:marinespecies.org:taxname:156134) lobster larvae were reared to the appropriate stage in lab under several different conditions.&nbsp;Most larvae were reared individually in an environmental control room at 18°C in 400 ml glass jars in 0.45 µm filtered seawater and fed fresh hatched brine shrimp ad libitum.&nbsp;Water changes were made every 2-3 days.&nbsp;Alternative rearing conditions included 14°C and fed fresh hatched brine shrimp, ambient seawater temperature (jars were held in a water bath of flow through seawater)&nbsp;fed fresh hatched brine shrimp, and 18°C and fed a diet of live, freshly collected zooplankton from local waters.&nbsp;We also conducted&nbsp;trials on wild caught stage IV larvae.&nbsp;Larvae were collected using a neuston net (0-0.5 m depth) in the vicinity of Boothbay, Maine, USA.&nbsp;Wild larvae were held in individual jars at ambient seawater temperature until trials could be conducted.&nbsp;</p>
<p>&nbsp;</p>
<p>To assess growth and mortality, larvae were transferred from their rearing condition to the treatment temperature and maintained at the treatment temperature until they either molted to the next developmental stage or died.&nbsp;Larvae were transferred in their jar at the rearing temperature to the treatment temperature without exchanging the water, which allowed for a gradual temperature change.&nbsp;Feeding and water changes continued as described for rearing conditions. When larvae molted to the next developmental stage, they were photographed for carapace length measurements and frozen individually.&nbsp;Measurements of carapace length&nbsp;were made from the posterior edge of the ocular cavity to the midline of the posterior edge of the carapace using ImageJ. Frozen larvae were subsequently dried for 24 hours at 60 °C and weighed.&nbsp; Larvae dying during the experiment were not measured or weighed.</p>
Specified by the Principal Investigator(s)
- Data from 2021 and 2022 were merged from two original data files into one data table represented by the primary data file of this dataset. Year data is still retained within the HATCH_OR_CAPTURE_DATE column of this data file.
- Units were removed from column names (unit information can be found in the parameters section of the BCO-DMO metadata page).
- Spaces in column names replaced with underscores ("_").
- Standardized bit character representation of blank values within the data file.
- Removed "1 claw" note from dry weight field and added this to the notes column.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Nikon SMZ745T
Nikon SMZ745T
PI Supplied Instrument Name: Nikon SMZ745T PI Supplied Instrument Description:A Nikon SMZ745T with an AmScope HD205-WU camera, ImageJ was used to take photographs and make the larval measurements. Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/