http://lod.bco-dmo.org/id/dataset/939970
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-10-09
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Adenosine triphosphate and microbial biomass measurements from the Chesapeake Bay, sampled aboard RV Fay Slover on April 11, 2018
2024-12-30
publication
2024-12-30
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-01-27
publication
https://doi.org/10.26008/1912/bco-dmo.939970.1
Alexander Boris Bochdansky
Old Dominion University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Bochdansky, A. B. (2025) Adenosine triphosphate and microbial biomass measurements from the Chesapeake Bay, sampled aboard RV Fay Slover on April 11, 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-12-30 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.939970.1 [access date]
ATP & micriobial biomass, Chesapeake Bay 2018 Dataset Description: Methods and Sampling: <p><u><strong>Field measurements and bottle collections:</strong> </u>Seawater was collected on the research vessel Fay Slover using 5 L Niskin bottles along a gradient from the open ocean near the Chesapeake light tower into the Chesapeake Bay and the James River, in April 2018 and June 2019. Conductivity, temperature, and depth were measured using a SBE 32 CTD equipped with a Wetlabs fluorometer and a Wetlabs transmissometer (650 nm).</p>
<p><u><strong>Field collection of ATP:&nbsp;</strong> </u>ATP samples were processed using the hot-water extraction method described by Bochdansky et al. (2021). Three types of ATP samples were collected: 1) pATP in 5 ml seawater filtered through 0.2 mm pore size polycarbonate filters (Isopore GTTP, 25 mm diam.), 2) dissolved ATP (ATP in the filtrate of 0.2 mm filtration), and 3) 0.5 ml ATP in whole water without filtration. Polycarbonate filters were chosen over GF/F filters because of their higher retention efficiency (Taguchi and Laws, 1988; Lee et al., 1995) and because the volumes filtered were small, to ensure filtration times were kept to a minimum even with the use of the smaller pore size. Five milliliters of each depth were immediately and simultaneously filtered using a filtration manifold (25 mm-diameter filters, stainless steel screen supports) preloaded with the polycarbonate filters. The filters were placed in 15 ml polypropylene centrifuge tubes (Falcon<sup>TM</sup>) within seconds after the water passed. The dissolved ATP was captured in 15 ml polypropylene centrifuge vials placed underneath the filtration manifold and inside the vacuum flasks. The dissolved ATP samples (i.e., the 0.2 mm filtrates) were immersed in a boiling water bath for ~15 minutes immediately after filtration to sterilize the water and inactivate ATPases and then frozen at -80 °C until analysis.</p>
<p>In April 2018, approximately 4.5 ml of boiling ultrapure water was quickly added to each centrifuge tube. The tubes were stoppered and transferred to a beaker with boiling water for ~15 minutes for extraction of intracellular ATP and inactivation of ATPases. After extraction, the tubes were cooled to room temperature and then kept frozen at -20 °C for transport to the laboratory. Samples were subsequently kept at -80 °C until analysis.</p>
<p>Hot-water extraction was then performed on the still frozen samples (i.e., without prior thawing) in the laboratory (see Bochdansky et al. 2021 for details). The shock freezing-boiling treatment breaks up cells more efficiently than boiling alone, which results in higher extraction efficiencies (Bochdansky et al. 2021).</p>
<p>In 2018, 500 microliters of water from each bottle was collected and extracted in a boiling hot-water bath before the samples were cooled to room temperature, and then frozen at -20 °C. In 2019, 500 microliters<strong> </strong>of water from each bottle was placed in 15 ml centrifuge tubes and shock-frozen in liquid nitrogen. These unfiltered samples thus contained both particulate and dissolved ATP and were labeled total ATP (tATP). All samples were brought to the laboratory in a -20 °C freezer and subsequently stored at -80 °C in the laboratory.</p>
<p>For analysis of the 500 microliters shock-frozen samples, boiling hot water was added to the samples and extracted for ~15 minutes in a boiling-water bath.</p>
<p>Particulate and total ATP samples were topped up to 5 ml with ultrapure water using the gradations on the centrifuge tubes and mixed with a vortex mixer. The 500 microliters whole-water samples were also diluted to 5 ml with ultrapure water to reduce salt effects that strongly decrease the luminescence yield. It should be noted that the whole-water extraction method used here (for total ATP) requires sufficiently high ATP levels to produce a signal. This was possible because samples were taken in the mesotrophic coastal ocean and in a eutrophic estuary. Such a small amount of water (500 ml) would be insufficient in oligotrophic or deep-sea environments. Hot-water extraction is only one of two methods proposed by Bochdansky et al. (2021). Many of our subsequent collections were based on chemical extraction using phosphorus benzalkonium chloride (P-BAC) instead. Both methods give highly consistent results, with the values from the chemical extraction method exceeding that of the hot water extraction by 20% (Bochdansky et al. 2021). The hot-water method used here has the advantage that measurements of dissolved ATP can be added easily to the protocol as hot water for the inactivation of ATPases is already at hand.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2319114 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2319114
completed
Alexander Boris Bochdansky
Old Dominion University
757-683-4933
Ocean, Earth and Atmospheric Sciences 4600 Elkhorn Ave.
Norfolk
VA
23529
USA
abochdan@odu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
latitude
longitude
station
depth
ATPtot
Prokaryote_carbon_fg_ml
Eukayote_carbon_fg_ml
temperature_Celsius
chlorophyll_rfu
beamc
salinity_psu
sigma_t
SBE 32 CTD
Olympus BC61 epifluorescence microscope
Wetlabs fluorometer
Europa 20 20
perkinElmer Liquid Scintillation Analyzer
Wetlabs transmissiometer
theme
None, User defined
date
latitude
longitude
station
depth
Adenosinetriphosphate
Elemental carbon (C)
water temperature at measurement depth
fluorescence
beam attenuation
salinity
sigma-t
featureType
BCO-DMO Standard Parameters
CTD Sea-Bird
Fluorescence Microscope
Fluorometer
Isotope-ratio Mass Spectrometer
Liquid Scintillation Counter
Niskin bottle
Transmissometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Adenosine triphosphate as a master variable for biomass in the oceanographic context
https://osprey.bco-dmo.org/project/917950
Adenosine triphosphate as a master variable for biomass in the oceanographic context
<p><em>NSF Award Abstract:</em><br />
In the ocean, most living organisms are microbes that are too small to be seen by the naked eye. Despite their small size, microbes play an important role in processes that govern marine ecosystems and food webs. For example, microbes affect the concentrations of nutrients and gases in the water and the atmosphere, thereby exerting a significant impact on the climate globally. Consequently, it is important to know how many microbes there are in any given environment because there is a direct causal connection between living mass and overall biological activity. Determining how “alive” any volume of water is, however, is a difficult task. The gold standard is to count microbial cells under the microscope. This method is extremely time consuming when done well and needs to be performed separately on many different types of microbial cells. In addition, standard microscopic techniques do not reveal whether the cells were alive when they were collected. In contrast, a chemical method based on the amount of adenosine triphosphate (ATP) offers distinct advantages. Notably, ATP is relatively easy to measure, and the method can be widely used because all living cells contain ATP in similar concentrations. This study tests and applies an improved method of ATP analysis to generate data at very high resolution in space and time. One PhD student and six undergraduate students will receive research training and the project fosters international research collaborations with European scientists. This research provides deeper insights into the distribution of live matter in different regions and depths of the world’s oceans.</p>
<p>Decades ago, adenosine triphosphate (ATP) was proposed as a universal biomass indicator. However, its application in the field of oceanography has been limited due to misconceptions regarding cellular ATP concentration. Recent evidence suggests that ATP functions as a hydrotrope requiring homeostatically controlled ATP levels much higher than those solely needed for energy metabolism. ATP occurs in surprisingly stable concentrations in cytoplasm across a wide range of microbes thus representing live cytoplasm volume. This project examines in detail the usefulness of particulate ATP (PATP) as a biomass marker over a large section of the North Atlantic Ocean with special emphasis on mesopelagic and deep-sea environments where chlorophyll is a poor indicator of biomass or associated biological processes. The project uses field collections of marine snow and ambient water in combination with particle cameras to examine the microscale heterogeny of biomass in the water column. Laboratory studies determine factors that may influence the recovery of PATP through filtration and extraction protocols and determine to what extent ATP concentrations potentially deviate from the typical cytoplasm concentration during phosphorus limitation. The improved PATP-biomass method offers numerous operational advantages, especially the fact that it can be employed at high spatial and temporal resolution. Once validated, the PATP biomass method could be widely adopted as a key variable for biomass in routine oceanographic surveys. This project supports graduate and undergraduate students from diverse backgrounds to contribute to laboratory and field research. Public outreach efforts include tours and presentations for middle and high-school students, as well as the general public.</p>
<p>This project is funded by the Chemical Oceanography and Biological Oceanography Programs in the Division of Ocean Sciences.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
ATP biomass indicator
largerWorkCitation
project
eng; USA
oceans
-76.22801167
-75.66680167
36.90872833
37.00831167
2018-04-11
2018-04-11
Global
0
BCO-DMO catalogue of parameters from Adenosine triphosphate and microbial biomass measurements from the Chesapeake Bay, sampled aboard RV Fay Slover on April 11, 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/947579.rdf
Name: Date
Units: unitless
Description: <p>Sampling date in ISO format</p>
http://lod.bco-dmo.org/id/dataset-parameter/947580.rdf
Name: latitude
Units: decimal degrees
Description: <p>Sampling latitude, south is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/947581.rdf
Name: longitude
Units: decimal degrees
Description: <p>Sampling longitude, west is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/947582.rdf
Name: station
Units: unitless
Description: <p>Station station number during cruise</p>
http://lod.bco-dmo.org/id/dataset-parameter/947583.rdf
Name: depth
Units: meters (m)
Description: <p>Sampling depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/947584.rdf
Name: ATPtot
Units: unitless
Description: <p>Total ATP (whole water)</p>
http://lod.bco-dmo.org/id/dataset-parameter/947585.rdf
Name: Prokaryote_carbon_fg_ml
Units: femtogram/ml
Description: <p>Microscope estimates of prokaryote carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/947586.rdf
Name: Eukayote_carbon_fg_ml
Units: femtogram/ml
Description: <p>Microscope estimates of eukaryote carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/947587.rdf
Name: temperature_Celsius
Units: degrees Celsius (°C)
Description: <p>Temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/947588.rdf
Name: chlorophyll_rfu
Units: relative fluorescence units
Description: <p>Chlorophyll fluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/947589.rdf
Name: beamc
Units: m-1
Description: <p>Beam attenuation</p>
http://lod.bco-dmo.org/id/dataset-parameter/947590.rdf
Name: salinity_psu
Units: psu
Description: <p>Salinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/947591.rdf
Name: sigma_t
Units: unitless
Description: <p>Sigma-t</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
4058
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/c8e79fab-27eb-442f-9dd5-3d6d1d1b3a36/content
download
https://doi.org/10.26008/1912/bco-dmo.939970.1
download
onLine
dataset
<p><u><strong>Field measurements and bottle collections:</strong> </u>Seawater was collected on the research vessel Fay Slover using 5 L Niskin bottles along a gradient from the open ocean near the Chesapeake light tower into the Chesapeake Bay and the James River, in April 2018 and June 2019. Conductivity, temperature, and depth were measured using a SBE 32 CTD equipped with a Wetlabs fluorometer and a Wetlabs transmissometer (650 nm).</p>
<p><u><strong>Field collection of ATP:&nbsp;</strong> </u>ATP samples were processed using the hot-water extraction method described by Bochdansky et al. (2021). Three types of ATP samples were collected: 1) pATP in 5 ml seawater filtered through 0.2 mm pore size polycarbonate filters (Isopore GTTP, 25 mm diam.), 2) dissolved ATP (ATP in the filtrate of 0.2 mm filtration), and 3) 0.5 ml ATP in whole water without filtration. Polycarbonate filters were chosen over GF/F filters because of their higher retention efficiency (Taguchi and Laws, 1988; Lee et al., 1995) and because the volumes filtered were small, to ensure filtration times were kept to a minimum even with the use of the smaller pore size. Five milliliters of each depth were immediately and simultaneously filtered using a filtration manifold (25 mm-diameter filters, stainless steel screen supports) preloaded with the polycarbonate filters. The filters were placed in 15 ml polypropylene centrifuge tubes (Falcon<sup>TM</sup>) within seconds after the water passed. The dissolved ATP was captured in 15 ml polypropylene centrifuge vials placed underneath the filtration manifold and inside the vacuum flasks. The dissolved ATP samples (i.e., the 0.2 mm filtrates) were immersed in a boiling water bath for ~15 minutes immediately after filtration to sterilize the water and inactivate ATPases and then frozen at -80 °C until analysis.</p>
<p>In April 2018, approximately 4.5 ml of boiling ultrapure water was quickly added to each centrifuge tube. The tubes were stoppered and transferred to a beaker with boiling water for ~15 minutes for extraction of intracellular ATP and inactivation of ATPases. After extraction, the tubes were cooled to room temperature and then kept frozen at -20 °C for transport to the laboratory. Samples were subsequently kept at -80 °C until analysis.</p>
<p>Hot-water extraction was then performed on the still frozen samples (i.e., without prior thawing) in the laboratory (see Bochdansky et al. 2021 for details). The shock freezing-boiling treatment breaks up cells more efficiently than boiling alone, which results in higher extraction efficiencies (Bochdansky et al. 2021).</p>
<p>In 2018, 500 microliters of water from each bottle was collected and extracted in a boiling hot-water bath before the samples were cooled to room temperature, and then frozen at -20 °C. In 2019, 500 microliters<strong> </strong>of water from each bottle was placed in 15 ml centrifuge tubes and shock-frozen in liquid nitrogen. These unfiltered samples thus contained both particulate and dissolved ATP and were labeled total ATP (tATP). All samples were brought to the laboratory in a -20 °C freezer and subsequently stored at -80 °C in the laboratory.</p>
<p>For analysis of the 500 microliters shock-frozen samples, boiling hot water was added to the samples and extracted for ~15 minutes in a boiling-water bath.</p>
<p>Particulate and total ATP samples were topped up to 5 ml with ultrapure water using the gradations on the centrifuge tubes and mixed with a vortex mixer. The 500 microliters whole-water samples were also diluted to 5 ml with ultrapure water to reduce salt effects that strongly decrease the luminescence yield. It should be noted that the whole-water extraction method used here (for total ATP) requires sufficiently high ATP levels to produce a signal. This was possible because samples were taken in the mesotrophic coastal ocean and in a eutrophic estuary. Such a small amount of water (500 ml) would be insufficient in oligotrophic or deep-sea environments. Hot-water extraction is only one of two methods proposed by Bochdansky et al. (2021). Many of our subsequent collections were based on chemical extraction using phosphorus benzalkonium chloride (P-BAC) instead. Both methods give highly consistent results, with the values from the chemical extraction method exceeding that of the hot water extraction by 20% (Bochdansky et al. 2021). The hot-water method used here has the advantage that measurements of dissolved ATP can be added easily to the protocol as hot water for the inactivation of ATPases is already at hand.</p>
Specified by the Principal Investigator(s)
<p><strong><u>Laboratory analysis of ATP : </u></strong>Fifty microliters of each sample (in triplicates) were transferred to 6 ml pony scintillation vials (Research Products International), and received 3 ml of ultrapure water, and 50 µL of CellTiter-Glo 2.0 (Promega Corporation). Internal standards were used by spiking a fourth vial with 50 µL of samples with 50 µL of 0.0164 µM ATP standard. Using internal standards instead of separate calibration curves corrects for matrix effects that change the luminescence signal caused by the presence of ions, acids, and organic material (Bochdansky et al. 2021). &nbsp;Luminescence was analyzed in a PerkinElmer Liquid Scintillation Analyzer with a single photon counting protocol of 1 minute each. The counter was programmed to cycle samples five times in sequence. We determined that values from the second cycle were the most consistent and were thus subsequently used for all analyses.</p>
<p>See supplemental files for formula to calculate ATP, also available in Bochdansky et al. 2021.</p>
<p><strong><u>Microscopic analysis of biomass, 2018 samples: </u></strong>For bacteria abundance, 20 ml of the Niskin bottle sample was added to a 50 ml Falcon tube and preserved with 2% (fin. conc.) formaldehyde. Within 24 hours, 5 ml of the formaldehyde sample (in duplicates) was filtered onto a 25 mm diameter, 0.2 mm pore size, black polycarbonate filter (Isopore type GTBP, Millipore Corp.). The filter was put onto a slide and one drop of Vectashield Antifade Mounting Medium with DAPI (H1200, Vector Laboratories) was applied. A cover slip was added and the slide was stored at -20 °C.</p>
<p>The slides were brought to room temperature in a desiccator to remove condensation before immersion oil was added to the cover slips. Bacteria were enumerated under an Olympus BX61 epifluorescence microscope (100 x oil immersion objective, 2 x loupe, 10 x ocular magnification). ToupView (Toup Tek Photonics) imaging software was used to take images of the bacteria slides. Images were analyzed using a custom macro developed for ImageJ software (National Institutes of Health). This macro inverted the image, converted it into an 8 bit image, applied a FFT Bandpass Filter, binarized the image, and finally applied a watershed feature that outlined each bacterium. The algorithm automatically counted the number of bacteria and the area of each bacterium on each picture. A fixed value of 20 femtogram (fg) carbon per prokaryotic cell was applied (Ducklow, 2018). We preferred the higher value as cell carbon is typically higher in eutrophic systems than in open and deep ocean environments (Ducklow, 2018; Herndl et al., 2023).&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
<p>For the enumeration of eukaryotes and measurements of size, two types of analysis were performed: epifluorescence and inverted microscopy. The same slides used for the bacteria counts were also used to count eukaryote microbes directly. The eukaryotes were counted if the bright blue spots were at least twice the size (linear dimension) of the bacteria in the image. If a large red fluorescence due to chlorophyll was noticed but there was no noticeable blue nucleus, it was still counted as a eukaryote. Organisms that were completely inside the picture were counted; if an organism landed on the top or left side of the picture and at least ¾ of the organism was visible, then it was counted too. If the organism landed on the right or the bottom of the image and was cut off, then it was not counted. The total number of eukaryotes was taken for each station and depth and a fixed value of 2,200 fg was applied, assuming that the overwhelming majority of these eukaryotes were nanoplankton (Pomeroy 1974, Fukuda et al., 2007; Sohrin et al., 2010). Given the wide size range in eukaryotes, this step represents a majr simplification.</p>
<p>For inverted microscopy, 20 ml sample vials were filled with seawater to the vial shoulder and 6 drops of Lugol’s solution were added to produce a tea-colored solution. Once in the lab, samples were mixed by inversion and 10 ml of the sample was measured into a graduated cylinder. Another 4 drops of Lugol’s solution were added and then topped off with equal-salinity 0.2 mm-filtered artificial seawater. Once the chamber was topped off, a glass plate was placed on the top to make it air-tight and to hold the water column in the settling chamber. After 24–48 hours of settling, the chamber was drained and replaced with a cover slip.</p>
<p>The slide was then processed under an Olympus CK7 inverted microscope equipped with a digital camera (see above). Organisms on the entire slide were imaged and all of those with the following criteria were analyzed. Organisms that were over 9,000 pixels in area (~ 100 mm<sup>2</sup>) and had a smooth shape to the structure (indicating a cell membrane) were included. For ciliated cells, only the area inside the cell membrane was measured. Diatom cell chains were measured as one composite organism. Each organism on the image was outlined and the area in pixels was recorded. If two or more organisms were identical, then only one would be measured and that measurement would be extrapolated to all identical organisms on the image. Any empty-shelled organisms were excluded. Due to time constraints, only approximately every tenth image was examined, and the results extrapolated to the entire sample. The area in pixels was converted to mm<sup>2</sup> then the volume was calculated according to Bochdansky et al. (2017a). Volumes were converted into carbon values using equation 2 (Menden-Deuer and Lessard, 2000):</p>
<p>Log<sub>10</sub>(carbon (pg)) = -0.665+0.939*Log<sub>10</sub>(volume (mm<sup>3</sup>)</p>
Specified by the Principal Investigator(s)
* Split methodology and data processing between 2 datasets. (review from submitter).
* Add sampling date to dataset.
* Adjusted parameter names to comply with database requirements.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
SBE 32 CTD
SBE 32 CTD
PI Supplied Instrument Name: SBE 32 CTD Instrument Name: CTD Sea-Bird Instrument Short Name:CTD Sea-Bird Instrument Description: A Conductivity, Temperature, Depth (CTD) sensor package from SeaBird Electronics. This instrument designation is used when specific make and model are not known or when a more specific term is not available in the BCO-DMO vocabulary. Refer to the dataset-specific metadata for more information about the specific CTD used. More information from: http://www.seabird.com/ Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
Olympus BC61 epifluorescence microscope
Olympus BC61 epifluorescence microscope
PI Supplied Instrument Name: Olympus BC61 epifluorescence microscope Instrument Name: Fluorescence Microscope Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/
Wetlabs fluorometer
Wetlabs fluorometer
PI Supplied Instrument Name: Wetlabs fluorometer Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
Europa 20 20
Europa 20 20
PI Supplied Instrument Name: Europa 20 20 Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
perkinElmer Liquid Scintillation Analyzer
perkinElmer Liquid Scintillation Analyzer
PI Supplied Instrument Name: perkinElmer Liquid Scintillation Analyzer Instrument Name: Liquid Scintillation Counter Instrument Short Name:LSC Instrument Description: Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used to quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.
Liquid scintillation counters are instruments assaying alpha and beta radiation by quantitative detection of visible light produced by the passage of rays or particles through a suitable scintillant incorporated into the sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/
PI Supplied Instrument Name: Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Wetlabs transmissiometer
Wetlabs transmissiometer
PI Supplied Instrument Name: Wetlabs transmissiometer Instrument Name: Transmissometer Instrument Short Name:Transmissometer Instrument Description: A transmissometer measures the beam attenuation coefficient of the lightsource over the instrument's path-length. This instrument designation is used when specific manufacturer, make and model are not known. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/124/