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            <gco:CharacterString>Cite this dataset as: Cowen, R. K., Conser, E., Sponaugle, S., Sutherland, K. R., Corrales Ugalde, M. (2024) Environmental sensor data from an underwater imaging system (ISIIS-3) collected during R/V Langseth cruise MGL2207 July 20-28 2022 and R/V Sally Ride cruise SR2317 August 10-20 2023 in the Northern California Current. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-11-01 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.942686.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Dataset Description: &amp;lt;p&amp;gt;This dataset will be updated to include additional data from deployments from the&amp;amp;nbsp;winter of&amp;amp;nbsp;2022 and winter of 2023.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Location description:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;Six cross-shelf transects in the northern California Current, ranging from near the Oregon-California border (42.5°N) to southern Washington (47.1°N). Maximum depth of instrument is 100m or within 2-5m of the seafloor. A list of more detailed information about the individual transects is included (See Supplemental Files).&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;ISIIS-3 was deployed at night from the research vessel, and towed the length of the transects in an undulating manner at 2.5 m/s (vessel speed 5 kts), undulating between 1m and 100m depth, or within 2m of the seafloor in waters shallower than 100m depth. A total of six transects were sampled per cruise, with the longest transects split into inshore and offshore portions sampled over two nights. Each transect was sampled in the “tow-yo” fashion throughout the deployment of the instrument. ISIIS-3 was terminated to a fiber optic winch. Data were streamed to the shipboard ISIIS-3 computer in real time. A program manager (JADE, https://sixclear.com/jade/) took the input data streams from both cameras, each sensor, and GPS data from the ship, and wrote into individual millisecond time-stamped files. Data were stored on the shipboard computer’s 16TB internal drives, then copied on to individual 6TB external drives in duplicate for each camera. Individual files were written for the CTD, GPS stream, voltage-based sensor readings (pH, dissolved oxygen, PAR), altimeter, and flowmeter, and files were written on the shipboard ISIIS-3 computer.&amp;amp;nbsp;&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
See&amp;amp;nbsp;Schmid et al. (2023, doi:10.3389/fmars.2023.1187771) for more information on ISIIS-3 imager and deployments on these research cruises.&amp;amp;nbsp;&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/855250.rdf" xlink:title="OCE-2125408" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2125408 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2125408</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/858394.rdf" xlink:title="OCE-2125407" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2125407 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2125407</gmx:Anchor>
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Marine plankton form the base of most ocean food webs that support valuable fisheries. This highly diverse and complex community is composed of organisms that drift with ocean currents. Planktonic organisms remain understudied: they are difficult to sample given that their sizes span more than six orders of magnitude from less than one micron to meters. Yet, understanding how these communities respond to climate change, and ultimately how these responses affect valuable fisheries, and therefore food security, is critical. Because many ecological and physiological processes are dictated by relative size, the theory of size spectra (i.e., the relationship between size and organism abundance as it drives ecosystem properties such as food webs) provides a valuable framework for forecasting climate change impacts on marine ecosystems. A deeper understanding of the scope and nature of variability in size spectra under contrasting environmental conditions is needed. The dynamic, highly productive northern California Current off Oregon and Washington, during the summer and winter seasons, produces a patchwork of oceanographic conditions including those associated with hypoxia and ocean acidification. This study is sampling the plankton communities in this region to investigate how gradients of temperature, nutrients, dissolved oxygen, and pH conditions impact size spectra. The broader impacts include the training of students, building scientific resources, and outreach to broader communities. Undergraduate and graduate students are being trained in oceanography, field research and new technologies. The automated image analysis pipeline developed as part of the project is openly accessible to the oceanographic community and the image data are available through the novel Global Plankton Imagery Library, an open-access repository for plankton imagery. Size spectra data from this study are shared directly with ecosystem modelers. The project’s flagship outreach activity is the collaboration with the Sitka Center for Art and Ecology and the hosting of an Artist-At-Sea Program. A professional artist is competitively selected to join the research cruises and to create artistic products that give a unique voice to oceanographic research and the organisms under study. The artwork is being assembled into a traveling public Art Exhibit with planned displays at the Sitka Center, Oregon State University’s Hatfield Marine Science Center, University of Oregon’s Charleston Marine Life Center and centers located in underserved coastal communities. Finally, imagery data from the project are being shared via the Plankton Portal, a public website developed in partnership with the Citizen Science Alliance’s Zooniverse, that invites citizen scientists to participate in classifying plankton images.&lt;/p&gt;
&lt;p&gt;The coupling of in situ plankton imagery and morphometric data allows quantifying scales of variation in plankton size spectra as well as testing predictions of how changes in environmental conditions (notably, temperature, nutrients, oxygen, pH) correlate with shifts in size spectra to reveal functional consequences to the food web. Plankton size spectra are being compared across environmental conditions by sampling in a habitat with steep environmental gradients and during two contrasting seasons. Planktonic organisms spanning 10 orders of magnitude in biomass are sampled using two complementary high-resolution imaging systems: the In Situ Ichthyoplankton Imaging System (ISIIS) and the Laser In-Situ Scattering and Transmissometry (LISST) particle imager. High-throughput image analysis software is used to create size distributions together with taxonomic classification. Depth-discrete meso-zooplankton samples are collected in parallel to examine community shifts in carbon, obtain length-to-carbon conversions and calibrate image data. The normalized biomass size spectra computed from the image data are tested for deviations from expected patterns. The plankton collections are also being analyzed for diet and reproductive status of gelatinous zooplankton, and diet and daily growth rate of representative larval fishes. These two groups have been historically understudied yet play central roles in ecosystem function. The data are being used to examine how these organisms are impacted by environmental conditions, and how they affect plankton size spectra. This study is foundational to the understanding of marine ecosystems within the context of climate change.&lt;/p&gt;
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            http://lod.bco-dmo.org/id/dataset-parameter/944044.rdf
	Name: Time
	Units: unitless
	Description: &lt;p&gt;Time of sampling, in time zone Pacific Time (PST/PDT). Measurements from individual sensors  merged based on millisecond timestamp using Python code&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944045.rdf
	Name: Cruise_ID
	Units: unitless
	Description: &lt;p&gt;Cruise identifier&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944046.rdf
	Name: ISO_DateTime_UTC
	Units: unitless
	Description: &lt;p&gt;Time of sampling, in time zone (UTC).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944047.rdf
	Name: Temperature
	Units: degrees Celsius
	Description: &lt;p&gt;Water temperature, degrees Celsius, Sea-Bird SBE 49 FastCAT mounted on ISIIS-3 . Quality flag is in the column &amp;#039;Temperature_flag&amp;#039;, see column description for more information about what data are flagged.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944048.rdf
	Name: Conductivity
	Units: Siemens per meter (Siemens/m)
	Description: &lt;p&gt;Water conductivity, measured using SeaBird SBE 49 FastCAT mounted on ISIIS-3. Quality flag is in &amp;#039;Conductivity_flag&amp;#039;, see column description for more information about what data are flagged&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944049.rdf
	Name: Pressure
	Units: decibars (dBar)
	Description: &lt;p&gt;Pressure measured using SeaBird SBE 49 FastCat mounted on ISIIS-3. Quality flag is in &amp;#039;Pressure_flag&amp;#039;, see column description for more information about what data are flagged&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944050.rdf
	Name: Depth
	Units: meters (m)
	Description: &lt;p&gt;Depth measured using SeaBird SBE 49 FastCat mounted on ISIIS-3. Quality flag is 'Depth_flag', see column description for more information about what data are flagged&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944051.rdf
	Name: Salinity
	Units: Practical Salinity Units (PSU)
	Description: &lt;p&gt;Salinity measured using SeaBird SBE 49 FastCat mounted on ISIIS-3. Quality flag is &amp;#039;Salinity_flag&amp;#039;, see column description for more information about what data are flagged&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944052.rdf
	Name: Altitude
	Units: meters (m)
	Description: &lt;p&gt;Imagenex Model 850 Altimeter mounted on ISIIS-3. Altitude of ISIIS-3 instrument from seafloor. Only measures within 50m of seafloor, values where ISIIS-3 is greater than 50m above seafloor are blank values (null).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944053.rdf
	Name: Horizontal_Speed
	Units: centimeters per second (cm/s)
	Description: &lt;p&gt;Relative horizontal speed of ISIIS instrument through the water, from Valeport Model 803 ROV Current Meter mounted on ISIIS-3. Quality flag is &amp;#039;Horizontal_Speed_flag,&amp;#039; see column description for more information about what data are flagged&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944054.rdf
	Name: Vertical_Speed
	Units: centimeters per second (cm/s)
	Description: &lt;p&gt;Relative vertical speed of ISIIS instrument through the water, from Valeport Model 803 ROV Current Meter mounted on ISIIS-3. Quality flag is &amp;#039;Vertical_Speed_flag,&amp;#039; see column description for more information about what data are flagged&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944055.rdf
	Name: Fluoro
	Units: counts
	Description: &lt;p&gt;Fluoroscence measured with Wet Labs FLRT mounted on ISIIS-3. Quality flag is &amp;#039;Fluoro_flag,&amp;#039; see column description for more information about what data are flagged&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944056.rdf
	Name: Latitude
	Units: decimal degrees
	Description: &lt;p&gt;Latitude from ship data streams. Merged with sensor data based on millisecond time stamp&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944057.rdf
	Name: Longitude
	Units: decimal degrees
	Description: &lt;p&gt;Longitude from ship data streams. Merged with sensor data based on millisecond time stamp&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944058.rdf
	Name: PAR
	Units: microEinsteins per square centimeter (uEinsteins per cm^2)
	Description: &lt;p&gt;Photosynthetically active radiation, Biospherical QCP-2300 mounted on ISIIS-3. Quality flag is &amp;#039;PAR_flag,&amp;#039; see column description for more information about what data are flagged&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944059.rdf
	Name: pH
	Units: unitless
	Description: &lt;p&gt;Water pH from SBE 18 mounted on ISIIS-3. Quality flag is &amp;#039;pH_flag,&amp;#039; see column description for more information about what data are flagged&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944060.rdf
	Name: Oxygen
	Units: milliliters per liter (ml/L)
	Description: &lt;p&gt;Dissolved oxygen from SBE 43 mounted with SBE 5M pump  on ISIIS-3. Quality flag is &amp;#039;Oxygen_flag&amp;#039; see column description for more information about data flagging&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944061.rdf
	Name: chl_a_ul
	Units: microliters per liter (uL/L)
	Description: &lt;p&gt;Conversion from fluorometer counts to chlorophyll-a. Relationship between fluorescence at chlorophyll-a from WetLabs calibration, calculated from a phytoplankton monoculture relationship. Quality flag is &amp;#039;chl_a_flag&amp;#039;, see column description for more information about what data are flagged&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944062.rdf
	Name: Temperature_flag
	Units: unitless
	Description: &lt;p&gt;Value is 1 where Depth is less than 1m or greater than 110m&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944063.rdf
	Name: Conductivity_flag
	Units: unitless
	Description: &lt;p&gt;Value is 1 where Pressure is less than 1dbar, or greater than 110 dbar, or blank value (null).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944064.rdf
	Name: Pressure_flag
	Units: unitless
	Description: &lt;p&gt;Value is 1 for salinity values &amp;lt;20 psu or blank value (null).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944065.rdf
	Name: Depth_flag
	Units: unitless
	Description: &lt;p&gt;Value is 1 where Conductivity &amp;lt; 3 S/m or blank value(null).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944066.rdf
	Name: Salinity_flag
	Units: unitless
	Description: &lt;p&gt;Value of 1 where blank value (null).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944067.rdf
	Name: Horizontal_Speed_flag
	Units: unitless
	Description: &lt;p&gt;Value of 1 where &amp;#039;Horizontal_Speed&amp;#039; is less than -500 cm/s, greater than 500 cm/s, or blank value (null).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944068.rdf
	Name: Vertical_Speed_flag
	Units: unitless
	Description: &lt;p&gt;Value of 1 where &amp;#039;Vertical_Speed&amp;#039; is greater than 4000 cm/s, less than 0 m/s or blank value (null).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944069.rdf
	Name: Fluoro_flag
	Units: unitless
	Description: &lt;p&gt;Value of 1 where Fluorometer value is blank value (null)., less than 0, or greater than 4000&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944070.rdf
	Name: PAR_flag
	Units: unitless
	Description: &lt;p&gt;Value of 1 where PAR is greater than 0.1 µE/s, less than 0 µE/s or blank values (null).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944071.rdf
	Name: pH_flag
	Units: unitless
	Description: &lt;p&gt;Value of 1 where pH is blank value (null) or less than 7&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944072.rdf
	Name: Oxygen_flag
	Units: unitless
	Description: &lt;p&gt;Value of 1 where blank value (null).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944073.rdf
	Name: chl_a_flag
	Units: unitless
	Description: &lt;p&gt;Value of 1 where chl_a_ul is blank value (null)., less than 0 microliters per liter, or greater than 45 microliters per liter&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/944074.rdf
	Name: flag_totals
	Units: unitless
	Description: &lt;p&gt;Total sum of all flags in data. Ranges from 0 to 12, where 0 has no quality flags, and 12 represents data that are the most likely to contain errors or extreme data to be highly scrutinized&lt;/p&gt; 
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&amp;lt;p&amp;gt;ISIIS-3 was deployed at night from the research vessel, and towed the length of the transects in an undulating manner at 2.5 m/s (vessel speed 5 kts), undulating between 1m and 100m depth, or within 2m of the seafloor in waters shallower than 100m depth. A total of six transects were sampled per cruise, with the longest transects split into inshore and offshore portions sampled over two nights. Each transect was sampled in the “tow-yo” fashion throughout the deployment of the instrument. ISIIS-3 was terminated to a fiber optic winch. Data were streamed to the shipboard ISIIS-3 computer in real time. A program manager (JADE, https://sixclear.com/jade/) took the input data streams from both cameras, each sensor, and GPS data from the ship, and wrote into individual millisecond time-stamped files. Data were stored on the shipboard computer’s 16TB internal drives, then copied on to individual 6TB external drives in duplicate for each camera. Individual files were written for the CTD, GPS stream, voltage-based sensor readings (pH, dissolved oxygen, PAR), altimeter, and flowmeter, and files were written on the shipboard ISIIS-3 computer.&amp;amp;nbsp;&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
See&amp;amp;nbsp;Schmid et al. (2023, doi:10.3389/fmars.2023.1187771) for more information on ISIIS-3 imager and deployments on these research cruises.&amp;amp;nbsp;&amp;lt;/p&amp;gt;</gco:CharacterString>
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** In the BCO-DMO data system missing data identifiers are displayed according to the format of data you access. For example, in csv files it will be blank (null) values. In Matlab .mat files it will be NaN values. When viewing data online at BCO-DMO, the missing value will be shown as blank (null) values.

* ISO DateTime with timezone (UTC) column added in ISO 8601 format (converted from local &amp;quot;US/Pacific&amp;quot; timestamp in PST/PDT time zone.

* Column &amp;quot;Cruise_ID&amp;quot; added.

* Lat,lon rounded to 5 decimal places as decided with data submitter.  pH PAR and oxygen also rounded to 5 decimal places when over 5 places.

* One value in Time column &amp;quot;2023-08-14 02:36:48&amp;quot; was corrected to &amp;quot;2023-08-14 02:36:48.000000&amp;quot; to match format of column.  The submitter verified that indeed it should be microseconds .000000 and was a real time that had the trailing zeroes removed during processing workflow.</gco:CharacterString>
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            <gco:CharacterString>PI Supplied Instrument Name:  PI Supplied Instrument Description:Instrumentation associated wth ISIIS-3 are listed in the &quot;Instruments&quot; section of this dataset page except:
Two linescan cameras (55 µm/pixel resolution, Bellamare ISIIS-DPI) **imagery data is not associated with this dataset &quot;ISIIS Environmental Data&quot; Instrument Name: In Situ Ichtyoplankton Imaging System Instrument Short Name:ISIIS   Instrument Description: The In Situ Ichthyoplankton Imaging System (ISIIS) is an underwater imaging system aimed at capturing in situ, real time images of marine zooplankton of relatively low abundance such as fish larvae and fragile gelatinous organisms. The first prototype, delivered in 2007, was attached to a relatively simple vehicle towed by an oceanographic vessel at a speed of five knots. The vehicle, and associated imaging system and sensors, was moved up and down through the water column by paying cable in and out via an oceanographic winch. Subsequently, a new vehicle has been designed with the capacity of self undulation using motor actuated dive fins. 

The ISIIS system utilizes a high-resolution line-scanning camera with a Light Emitting Diode (LED) light source, modified by plano-convex optics, to create a collimated light field to back-light a parcel of water.

ISIIS was developed in collaboration between the University of Miami's Rosenstiel School of Atmospheric and Marine Science (RSMAS) and the subsea engineering company, Bellamare, LLC, located in San Diego CA. See complete description from RSMAS.

Reference:
Cowen RK and Guigand CM. 2008. In situ Ichthyoplankton Imaging System (ISIIS): system design and preliminary results. Limnol. Oceanogr. Methods. 6:126-132. doi:10.4319/lom.2008.6.126</gco:CharacterString>
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            <gco:CharacterString>PI Supplied Instrument Name: pH sensor (Seabird SBE 18) PI Supplied Instrument Description:Instrumentation associated with ISIIS-3. Instrument Name: pH Sensor Instrument Short Name:pH Sensor   Instrument Description: An instrument that measures the hydrogen ion activity in solutions.

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            <gco:CharacterString>PI Supplied Instrument Name: Photosynthetically active radiation/irradiance (Biospherical QCP-2300) PI Supplied Instrument Description:Instrumentation associated with ISIIS-3. Instrument Name: Photosynthetically Available Radiation Sensor Instrument Short Name:PAR sensor   Instrument Description: A PAR sensor measures photosynthetically available (or active) radiation.  The sensor measures photon flux density (photons per second per square meter) within the visible wavelength range (typically 400 to 700 nanometers). PAR gives an indication of the total energy available to plants for photosynthesis.  This instrument name is used when specific type, make and model are not known. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/122/</gco:CharacterString>
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  Instrument Name: Sea-Bird SBE 49 FastCAT CTD Sensor Instrument Short Name:SBE-49   Instrument Description: The SBE 49 FastCAT is a CTD sensor for use in autonomous platforms. It contains a SBE 3P temperature sensor, a SBE 4C conductivity sensor and a strain-gauge pressure sensor as standard. It can operate in autonomus (16 Hz per sec) or polled mode (transmits each sample). The sensor is depth-rated to 350 m (plastic housing) or 7000 m (titanium housing). Accuracy: +/- 0.002 deg C (temperature), +/- 0.0003 S/m (conductivity), 0.1% of full scale range (pressure).</gco:CharacterString>
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