http://lod.bco-dmo.org/id/dataset/942884 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2024-11-05 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Biogeochemical and microbial parameters collected during spatial surveys on a coral reef at Mo'orea's North Shore (French Polynesia) in September 2017, May 2019, and April 2022 2024-12-06 publication 2024-12-06 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2024-12-06 publication https://doi.org/10.26008/1912/bco-dmo.942884.1 Lihini Aluwihare University of California-San Diego Scripps principalInvestigator Craig E. Nelson University of Hawaiʻi at Mānoa principalInvestigator Linda Wegley Kelly University of California-San Diego Scripps principalInvestigator Irina Koester University of California-San Diego Scripps principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Koester, I., Aluwihare, L., Wegley Kelly, L., Nelson, C. E. (2024) Biogeochemical and microbial parameters collected during spatial surveys on a coral reef at Mo'orea's North Shore (French Polynesia) in September 2017, May 2019, and April 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-12-06 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.942884.1 [access date] Biogeochemical Measurements from Surface Waters at the North Shore of Mo'orea, French Polynesia Dataset Description: Methods and Sampling: &lt;p&gt;&lt;strong&gt;Sample Collection:&lt;/strong&gt;&lt;br /&gt; Surface water was sampled at Mo'orea's North Shore (French Polynesia) (-17.47722, -149.84258) mid-day aboard kayaks or small motor boats in September 2017, May 2019, and April 2022. At every station on the backreef, salinity and temperature were measured using a YSI Professional Plus (Yellow Springs Instruments). Seawater samples were collected directly into 5-liter (L) polycarbonate carboys from the surface waters approximately 30 centimeters (cm) below the seawater surface and immediately transported to the shore for further processing. One milliliter (mL) of whole seawater was collected by pipette and added to a cryovial with 16 microliters (µL) of 32% paraformaldehyde, mixed by inversion, and immediately frozen and stored at -40 degrees Celsius (°C) for subsequent flow cytometric analysis of plankton abundance. Subsequent samples were processed using a multi-channel peristaltic pump and acid-washed silicone tubing initially flushed with approximately 300 mL seawater sample. For microbial community composition analysis, 300 to 500 mL sample was filtered through 0.2-micrometer (µm) polyethersulfone filter cartridges (Sterivex, Millipore, UK) and frozen at -40°C. The sterivex filtrate was used to rinse bottles to collect samples for inorganic nutrients, which were frozen and stored at -40°C. For particulate organic matter samples, 3 to 4L of seawater samples were filtered through pre-combusted GF/F (25mm, Whatman) filters, folded into combusted aluminum foil, and frozen at -40°C. For DOC measurements, GF/F filtrate was used to triple sample-rinse borosilicate vials with Teflon septa caps before collecting 40 mL (for deployment 2 and 3 in duplicates). Additionally, 2 liters of filtrate were also collected in triple-rinsed polycarbonate bottles for solid-phase DOM extraction. In total, 3 to 5 liters of sample were filtered through this G/FF filter via peristaltic pumps using acid-cleaned silicone tubing for analysis of particulate organic matter (POM). The filters were folded in half, wrapped in aluminum foil, and promptly frozen and stored at -80°C until analysis. DOC and DOM samples were acidified to pH 2 using trace metal grade HCl. Duplicates of acidified 1L DOM samples were solid-phase extracted using the multichannel pump operating at a flow rate of 18 milliliters per minute (mL/min) onto Bond Elut PPL resin cartridges (200 milligrams (mg) bed mass, Agilent 2105005, USA), according to (Dittmar et al., 2008) and (Petras et al., 2017). After desalinating the resin with LC-MS grade water (Fisher Chemical, Belgium), the cartridges were dried using Ultra High Purity compressed N₂ gas and kept frozen at -40°C.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Biogeochemical Measurements:&lt;/strong&gt;&lt;br /&gt; The following inorganic nutrients were analyzed using a Seal AA3 Segmented Flow Injection Autoanalyzer at the University of Hawai'i SOEST Laboratory for Analytical Biogeochemistry: Nitrate+nitrite (N+N) and silicate concentrations (Grasshoff et al., 1983), ammonium (Kérouel and Aminot, 1997), phosphate (Murphy and Riley, 1962). Additionally, total dissolved nitrogen and total dissolved phosphorus were determined through separate injections, with UV and alkaline or acid persulfate in-line oxidation, respectively. DOC samples were analyzed using high-temperature platinum catalytic oxidation on a Shimadzu TOC-V at the University of Santa Barbara, according to (Carlson et al., 2010) for data collected in 2017 and 2019. Data collected in 2022 were analyzed at Scripps Institution of Oceanography according to &lt;a href=&quot;https://ccelter.ucsd.edu/dissolved-organic-carbon-and-total-nitrogen/&quot; target=&quot;_blank&quot;&gt;https://ccelter.ucsd.edu/dissolved-organic-carbon-and-total-nitrogen/&lt;/a&gt;. Particulate organic carbon (POC) and nitrogen (PON) concentrations were determined via filter combustion after acid fumigation to remove particulate inorganic carbon, drying, weighing, and packing into tin capsules (&lt;a href=&quot;https://ccelter.ucsd.edu/particulate-organic-carbon-and-nitrogen/&quot; target=&quot;_blank&quot;&gt;https://ccelter.ucsd.edu/particulate-organic-carbon-and-nitrogen/&lt;/a&gt;). Samples from 2017 were analyzed for carbon and nitrogen concentrations and stable isotope composition (δ13C and δ15N) at the SIO Stable Isotope Facility on a Thermo Finnigan DeltaPlus Isotope-Ratio mass spectrometer interfaced with a Costech 4010 elemental combustion analyzer. Filters from 2019 were analyzed on an Exeter Analytical CE 440 Elemental Analyzer in the SOEST Analytical Laboratory (&lt;a href=&quot;http://www.soest.hawaii.edu/S-LAB/&quot; target=&quot;_blank&quot;&gt;http://www.soest.hawaii.edu/S-LAB/&lt;/a&gt;). The analysis of fluorescent dissolved organic matter (fDOM) was conducted using a Horiba Aqualog scanning fluorometer, according to the methodology outlined in (Nelson et al., 2015). Samples for bacterioplankton enumeration were thawed and 200 µL of each sample was stained with SYBR Green I stain for a final concentration of 1X. Bacterial cell counts were enumerated using an Attune Acoustic Focusing Cytometer (Applied Biosystems, Part No. 4445280ASR) as described in (Nelson et al., 2015).&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2023509 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2023509 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2023298 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2023298 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2023707 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2023707 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2118618 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2118618 completed Lihini Aluwihare University of California-San Diego Scripps 858-822-4886 9500 Gilman Drive La Jolla CA 92093-0244 USA laluwihare@ucsd.edu pointOfContact Craig E. Nelson University of Hawaiʻi at Mānoa 805-705-3497 Center for Microbial Oceanography: Research & Education Dept of Oceanography & UH Sea Grant Honolulu HI 96822 USA cen@hawaii.edu pointOfContact Linda Wegley Kelly University of California-San Diego Scripps 858-246-4655 9500 Gilman Dr #0202 La Jolla CA 92093-0202 USA lwegley@ucsd.edu pointOfContact Irina Koester University of California-San Diego Scripps ikoester@ucsd.edu pointOfContact asNeeded Dataset Version: 1 Unknown Sample_ID Sampling_Strategy MassiveID MS_Sample_ID_1 MS_Sample_ID_2 Year Lon Lat DateTime_local ISO_DateTime_UTC ContactTime DOC Total_N Total_P Phosphate Silicate N_plus_N Ammonia PON POC PON15 POC13 Salinity Temperature PicoEukaryotes Prochlorococcus Synechococcus Heterotrophs M_C_ratio BIX HIX FI Ultra_Violet_Humic_like Marine_Humic_like Visible_Humic_like Tryptophan_like Tyrosine_like Phenylalanine_like Fulvic_Acid_like Optical_Brighteners Diesel_Band_II Petroleum_like Lignin_like PARAFAC1 PARAFAC2 PARAFAC3 PARAFAC4 PARAFAC5 PARAFAC6 Costech 4010 elemental combustion analyzer Exeter Analytical CE 440 Elemental Analyzer Attune Acoustic Focusing Cytometer Seal AA3 Segmented Flow Injection Autoanalyzer Horiba Aqualog scanning fluorometer Thermo Finnigan DeltaPlus Isotope-Ratio mass spectrometer Shimadzu TOC-V YSI Professional Plus theme None, User defined sample identification sampling_method year longitude latitude date ISO_DateTime_UTC time_elapsed dissolved organic Carbon Nitrogen Phosphorus phosphate Silicate, Si(OH)4, silicic acid nitrate plus nitrite Ammonia particulate organic nitrogen particulate organic Carbon (POC) d15N d13C salinity water temperature at measurement depth cell_concentration fDOM featureType BCO-DMO Standard Parameters Costech International Elemental Combustion System (ECS) 4010 Elemental Analyzer Flow Cytometer Flow Injection Analyzer Fluorometer Isotope-ratio Mass Spectrometer Shimadzu TOC-V Analyzer YSI Professional Plus Multi-Parameter Probe instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: Characterizing microbial transformation of marine DOM at the molecular level using untargeted metabolomic https://osprey.bco-dmo.org/project/931781 Collaborative Research: Characterizing microbial transformation of marine DOM at the molecular level using untargeted metabolomic <p><em>NSF Award Abstract:</em><br /> Dissolved organic matter is an important component of the global carbon cycle. Dissolved organic matter provides food and energy for microbes living in the ocean and influences microbial diversity. Microbes convert some dissolved organic matter to CO2 (respiration) whereas other forms of dissolved organic matter are altered by microbial processes and persist in the ocean. Thus, it is important to understand how microbes change dissolved organic matter composition and reactivity. This project will examine the chemical structure of dissolved organic matter to identify: 1) molecules that fulfill carbon demand (biomass produced minus losses from respiration) and 2) transformation processes that result from microbial activity. The project will combine lab experiments and field studies at the Moorea Coral Reef Long Term Ecological Research site. The project will support training for three graduate students in marine biogeochemistry. Undergraduate training is aimed at sustained mentoring of underrepresented minority (URM) students. Undergraduates will be recruited from existing programs at Minority Serving Institutions at San Diego State University and the University of Hawaiʻi at Mānoa. Undergraduates will participate in the Scripps Institution of Oceanography SURF Research Experiences for Undergraduates program, where they will conduct research in marine chemistry. The goal is to provide a mentoring approach that can successfully overcome roadblocks to URM engagement in STEM and increase retention of these students in marine science.</p> <p>This work will combine field and lab studies using advanced molecular-level chemical characterization tools to explore how bacteria alter the composition and bioreactivity of organic compounds dissolved in seawater. Additionally, this project will develop informatics-based tools to identify a larger proportion of chemical structures in marine dissolved organic matter (DOM) than is currently possible using traditional approaches. The project will use tandem mass spectrometry and networking techniques to comprehensively classify organic compounds into molecular families and determine common chemical transformations. Then, using a well-developed field-based experimental ecosystem to produce diverse labile DOM pools the research team will track microbial transformation using expression of hydrolytic enzymes and measure selection for particular microbial taxa and metabolisms. This approach defines the reactivity of individual molecules and broader compound classes participating in carbon fluxes that underpin DOM-microbe interactions. Field surveys conducted within the Moorea Coral Reef Long Term Ecological Research program will explore methods to track transformation of specific molecules in the environment and validate experimental observations of compound classes that appear to accumulate as semi-labile DOM. By integrating laboratory and field experiments and oceanographic surveys with the refinement of analytical tools for untargeted metabolomics, this project will characterize the fate of reactive DOM in the ocean.</p> Metabolomics on the Mo&#039;orea Reef largerWorkCitation project eng; USA oceans -149.8529899 -149.805 -17.50584 -17.4459786 2017-09-22 2022-04-12 Mo&#039;orea coral reefs 0 BCO-DMO catalogue of parameters from Biogeochemical and microbial parameters collected during spatial surveys on a coral reef at Mo'orea's North Shore (French Polynesia) in September 2017, May 2019, and April 2022 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/943085.rdf Name: Sample_ID Units: unitless Description: <p>Sample ID</p> http://lod.bco-dmo.org/id/dataset-parameter/943086.rdf Name: Sampling_Strategy Units: unitless Description: <p>Sampling strategy. Either "Spatial survey" and "Lagrangian survey".</p> <p>Spatial Survey = Samples were collected closely in time on Mo'orea's North Shore (French Polynesia) to provide a synoptic snapshot of the reef environment.<br /> Lagrangian Survey = Samples were collected during Lagrangian transect deployments. These followed the linear water flow from ocean-facing forereefs over a reef crest to the backreef lagoons.</p> http://lod.bco-dmo.org/id/dataset-parameter/943087.rdf Name: MassiveID Units: unitless Description: <p>Identification number for mass spectrometry data deposited on MassIVE (<a href="https://massive.ucsd.edu/" target="_blank">https://massive.ucsd.edu/</a>)</p> http://lod.bco-dmo.org/id/dataset-parameter/943088.rdf Name: MS_Sample_ID_1 Units: unitless Description: <p>File name of mass spectrometry data (LC-MS/MS; format: .raw and .mzML) for duplicate 1</p> http://lod.bco-dmo.org/id/dataset-parameter/943089.rdf Name: MS_Sample_ID_2 Units: unitless Description: <p>File name of mass spectrometry data (LC-MS/MS; format: .raw and .mzML) for duplicate 2</p> http://lod.bco-dmo.org/id/dataset-parameter/943090.rdf Name: Year Units: unitless Description: <p>4-digit year of sample collection</p> http://lod.bco-dmo.org/id/dataset-parameter/943091.rdf Name: Lon Units: decimal degrees Description: <p>Longitude of sample collection</p> http://lod.bco-dmo.org/id/dataset-parameter/943092.rdf Name: Lat Units: decimal degrees Description: <p>Latitude of sample collection</p> http://lod.bco-dmo.org/id/dataset-parameter/943093.rdf Name: DateTime_local Units: unitless Description: <p>Local date and time of sample collection</p> http://lod.bco-dmo.org/id/dataset-parameter/943094.rdf Name: ISO_DateTime_UTC Units: unitless Description: <p>Date and time (UTC) of sample collection in ISO 8601 format</p> http://lod.bco-dmo.org/id/dataset-parameter/943096.rdf Name: ContactTime Units: minutes Description: <p>Time after deployment for Lagrangian sample taken on the backreef</p> http://lod.bco-dmo.org/id/dataset-parameter/943097.rdf Name: DOC Units: micromoles per liter (umol/L) Description: <p>Dissolved Organic Carbon concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/943098.rdf Name: Total_N Units: micromoles per liter (umol/L) Description: <p>Total Nitrogen concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/943099.rdf Name: Total_P Units: micromoles per liter (umol/L) Description: <p>Total Phosphorus concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/943100.rdf Name: Phosphate Units: micromoles per liter (umol/L) Description: <p>Phosphate concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/943101.rdf Name: Silicate Units: micromoles per liter (umol/L) Description: <p>Silicate concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/943102.rdf Name: N_plus_N Units: micromoles per liter (umol/L) Description: <p>Nitrate + Nitrite concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/943103.rdf Name: Ammonia Units: micromoles per liter (umol/L) Description: <p>Ammonia concentration in water sample</p> http://lod.bco-dmo.org/id/dataset-parameter/943104.rdf Name: PON Units: micrograms per liter (ug/L) Description: <p>Particulate Organic Nitrogen based on GF/F filter analysis</p> http://lod.bco-dmo.org/id/dataset-parameter/943105.rdf Name: POC Units: micrograms per liter (ug/L) Description: <p>Particulate Organic Carbon based on GF/F filter analysis</p> http://lod.bco-dmo.org/id/dataset-parameter/943106.rdf Name: PON15 Units: per mil (‰) Description: <p>Stable nitrogen isotope ratio of Particulate Organic Nitrogen (?15N)</p> http://lod.bco-dmo.org/id/dataset-parameter/943107.rdf Name: POC13 Units: per mil (‰) Description: <p>Stable carbon isotope ratio of Particulate Organic Carbon (?13C)</p> http://lod.bco-dmo.org/id/dataset-parameter/943108.rdf Name: Salinity Units: practicial salinity units (PSU) Description: <p>Salinity</p> http://lod.bco-dmo.org/id/dataset-parameter/943109.rdf Name: Temperature Units: degrees Celsius Description: <p>Water temperature</p> http://lod.bco-dmo.org/id/dataset-parameter/943110.rdf Name: PicoEukaryotes Units: cells per microliter (cells/uL) Description: <p>PicoEukaryotes cell concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/943111.rdf Name: Prochlorococcus Units: cells per microliter (cells/uL) Description: <p>Prochlorococcus cell concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/943112.rdf Name: Synechococcus Units: cells per microliter (cells/uL) Description: <p>Synechococcus cell concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/943113.rdf Name: Heterotrophs Units: cells per microliter (cells/uL) Description: <p>Heterotrophic bacterioplankton concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/943114.rdf Name: M_C_ratio Units: Raman Fluorescence Units of Water Description: <p>Humic to Protein-like ratio</p> http://lod.bco-dmo.org/id/dataset-parameter/943115.rdf Name: BIX Units: Raman Fluorescence Units of Water Description: <p>Biological index</p> http://lod.bco-dmo.org/id/dataset-parameter/943116.rdf Name: HIX Units: Raman Fluorescence Units of Water Description: <p>Humification Index</p> http://lod.bco-dmo.org/id/dataset-parameter/943117.rdf Name: FI Units: Raman Fluorescence Units of Water Description: <p>Fluorescence Index</p> http://lod.bco-dmo.org/id/dataset-parameter/943118.rdf Name: Ultra_Violet_Humic_like Units: Raman Fluorescence Units of Water Description: <p>Ultra Violet Humic-like component</p> http://lod.bco-dmo.org/id/dataset-parameter/943119.rdf Name: Marine_Humic_like Units: Raman Fluorescence Units of Water Description: <p>Marine Humic-like component</p> http://lod.bco-dmo.org/id/dataset-parameter/943120.rdf Name: Visible_Humic_like Units: Raman Fluorescence Units of Water Description: <p>Visible Humic-like component</p> http://lod.bco-dmo.org/id/dataset-parameter/943121.rdf Name: Tryptophan_like Units: Raman Fluorescence Units of Water Description: <p>Tryptophan-like component</p> http://lod.bco-dmo.org/id/dataset-parameter/943122.rdf Name: Tyrosine_like Units: Raman Fluorescence Units of Water Description: <p>Tyrosine-like component</p> http://lod.bco-dmo.org/id/dataset-parameter/943123.rdf Name: Phenylalanine_like Units: Raman Fluorescence Units of Water Description: <p>Phenylalanine-like component</p> http://lod.bco-dmo.org/id/dataset-parameter/943124.rdf Name: Fulvic_Acid_like Units: Raman Fluorescence Units of Water Description: <p>Fulvic Acid-like component</p> http://lod.bco-dmo.org/id/dataset-parameter/943125.rdf Name: Optical_Brighteners Units: Raman Fluorescence Units of Water Description: <p>Optical Brighteners component</p> http://lod.bco-dmo.org/id/dataset-parameter/943126.rdf Name: Diesel_Band_II Units: Raman Fluorescence Units of Water Description: <p>Diesel Band II component</p> http://lod.bco-dmo.org/id/dataset-parameter/943127.rdf Name: Petroleum_like Units: Raman Fluorescence Units of Water Description: <p>Petroleum-like component</p> http://lod.bco-dmo.org/id/dataset-parameter/943128.rdf Name: Lignin_like Units: Raman Fluorescence Units of Water Description: <p>Lignin-like component</p> http://lod.bco-dmo.org/id/dataset-parameter/943129.rdf Name: PARAFAC1 Units: Raman Fluorescence Units of Water Description: <p>PARAFAC component 1</p> http://lod.bco-dmo.org/id/dataset-parameter/943130.rdf Name: PARAFAC2 Units: Raman Fluorescence Units of Water Description: <p>PARAFAC component 2</p> http://lod.bco-dmo.org/id/dataset-parameter/943131.rdf Name: PARAFAC3 Units: Raman Fluorescence Units of Water Description: <p>PARAFAC component 3</p> http://lod.bco-dmo.org/id/dataset-parameter/943132.rdf Name: PARAFAC4 Units: Raman Fluorescence Units of Water Description: <p>PARAFAC component 4</p> http://lod.bco-dmo.org/id/dataset-parameter/943133.rdf Name: PARAFAC5 Units: Raman Fluorescence Units of Water Description: <p>PARAFAC component 5</p> http://lod.bco-dmo.org/id/dataset-parameter/943134.rdf Name: PARAFAC6 Units: Raman Fluorescence Units of Water Description: <p>PARAFAC component 6</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 36775 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/540db2d0-9dd5-47b1-82a5-5317655d255a/content download https://doi.org/10.26008/1912/bco-dmo.942884.1 download onLine dataset &lt;p&gt;&lt;strong&gt;Sample Collection:&lt;/strong&gt;&lt;br /&gt; Surface water was sampled at Mo'orea's North Shore (French Polynesia) (-17.47722, -149.84258) mid-day aboard kayaks or small motor boats in September 2017, May 2019, and April 2022. At every station on the backreef, salinity and temperature were measured using a YSI Professional Plus (Yellow Springs Instruments). Seawater samples were collected directly into 5-liter (L) polycarbonate carboys from the surface waters approximately 30 centimeters (cm) below the seawater surface and immediately transported to the shore for further processing. One milliliter (mL) of whole seawater was collected by pipette and added to a cryovial with 16 microliters (µL) of 32% paraformaldehyde, mixed by inversion, and immediately frozen and stored at -40 degrees Celsius (°C) for subsequent flow cytometric analysis of plankton abundance. Subsequent samples were processed using a multi-channel peristaltic pump and acid-washed silicone tubing initially flushed with approximately 300 mL seawater sample. For microbial community composition analysis, 300 to 500 mL sample was filtered through 0.2-micrometer (µm) polyethersulfone filter cartridges (Sterivex, Millipore, UK) and frozen at -40°C. The sterivex filtrate was used to rinse bottles to collect samples for inorganic nutrients, which were frozen and stored at -40°C. For particulate organic matter samples, 3 to 4L of seawater samples were filtered through pre-combusted GF/F (25mm, Whatman) filters, folded into combusted aluminum foil, and frozen at -40°C. For DOC measurements, GF/F filtrate was used to triple sample-rinse borosilicate vials with Teflon septa caps before collecting 40 mL (for deployment 2 and 3 in duplicates). Additionally, 2 liters of filtrate were also collected in triple-rinsed polycarbonate bottles for solid-phase DOM extraction. In total, 3 to 5 liters of sample were filtered through this G/FF filter via peristaltic pumps using acid-cleaned silicone tubing for analysis of particulate organic matter (POM). The filters were folded in half, wrapped in aluminum foil, and promptly frozen and stored at -80°C until analysis. DOC and DOM samples were acidified to pH 2 using trace metal grade HCl. Duplicates of acidified 1L DOM samples were solid-phase extracted using the multichannel pump operating at a flow rate of 18 milliliters per minute (mL/min) onto Bond Elut PPL resin cartridges (200 milligrams (mg) bed mass, Agilent 2105005, USA), according to (Dittmar et al., 2008) and (Petras et al., 2017). After desalinating the resin with LC-MS grade water (Fisher Chemical, Belgium), the cartridges were dried using Ultra High Purity compressed N₂ gas and kept frozen at -40°C.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Biogeochemical Measurements:&lt;/strong&gt;&lt;br /&gt; The following inorganic nutrients were analyzed using a Seal AA3 Segmented Flow Injection Autoanalyzer at the University of Hawai'i SOEST Laboratory for Analytical Biogeochemistry: Nitrate+nitrite (N+N) and silicate concentrations (Grasshoff et al., 1983), ammonium (Kérouel and Aminot, 1997), phosphate (Murphy and Riley, 1962). Additionally, total dissolved nitrogen and total dissolved phosphorus were determined through separate injections, with UV and alkaline or acid persulfate in-line oxidation, respectively. DOC samples were analyzed using high-temperature platinum catalytic oxidation on a Shimadzu TOC-V at the University of Santa Barbara, according to (Carlson et al., 2010) for data collected in 2017 and 2019. Data collected in 2022 were analyzed at Scripps Institution of Oceanography according to &lt;a href=&quot;https://ccelter.ucsd.edu/dissolved-organic-carbon-and-total-nitrogen/&quot; target=&quot;_blank&quot;&gt;https://ccelter.ucsd.edu/dissolved-organic-carbon-and-total-nitrogen/&lt;/a&gt;. Particulate organic carbon (POC) and nitrogen (PON) concentrations were determined via filter combustion after acid fumigation to remove particulate inorganic carbon, drying, weighing, and packing into tin capsules (&lt;a href=&quot;https://ccelter.ucsd.edu/particulate-organic-carbon-and-nitrogen/&quot; target=&quot;_blank&quot;&gt;https://ccelter.ucsd.edu/particulate-organic-carbon-and-nitrogen/&lt;/a&gt;). Samples from 2017 were analyzed for carbon and nitrogen concentrations and stable isotope composition (δ13C and δ15N) at the SIO Stable Isotope Facility on a Thermo Finnigan DeltaPlus Isotope-Ratio mass spectrometer interfaced with a Costech 4010 elemental combustion analyzer. Filters from 2019 were analyzed on an Exeter Analytical CE 440 Elemental Analyzer in the SOEST Analytical Laboratory (&lt;a href=&quot;http://www.soest.hawaii.edu/S-LAB/&quot; target=&quot;_blank&quot;&gt;http://www.soest.hawaii.edu/S-LAB/&lt;/a&gt;). The analysis of fluorescent dissolved organic matter (fDOM) was conducted using a Horiba Aqualog scanning fluorometer, according to the methodology outlined in (Nelson et al., 2015). Samples for bacterioplankton enumeration were thawed and 200 µL of each sample was stained with SYBR Green I stain for a final concentration of 1X. Bacterial cell counts were enumerated using an Attune Acoustic Focusing Cytometer (Applied Biosystems, Part No. 4445280ASR) as described in (Nelson et al., 2015).&lt;/p&gt; Specified by the Principal Investigator(s) - Imported original file &quot;Moorea_NorthShore_Biogeochemistry.csv&quot; into the BCO-DMO system. - Marked &quot;NA&quot; as a missing data value (missing data are empty/blank in the final CSV file). - Renamed fields to comply with BCO-DMO naming conventions. - Converted the date-time columns to ISO 8601 format. - Saved final file as &quot;942884_v1_moorea_northshore_biogeochemistry.csv&quot;. Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Costech 4010 elemental combustion analyzer Costech 4010 elemental combustion analyzer PI Supplied Instrument Name: Costech 4010 elemental combustion analyzer Instrument Name: Costech International Elemental Combustion System (ECS) 4010 Instrument Short Name:Costech ECS 4010 Instrument Description: The ECS 4010 Nitrogen / Protein Analyzer is an elemental combustion analyser for CHNSO elemental analysis and Nitrogen / Protein determination. The GC oven and separation column have a temperature range of 30-110 degC, with control of +/- 0.1 degC. Exeter Analytical CE 440 Elemental Analyzer Exeter Analytical CE 440 Elemental Analyzer PI Supplied Instrument Name: Exeter Analytical CE 440 Elemental Analyzer Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/ Attune Acoustic Focusing Cytometer Attune Acoustic Focusing Cytometer PI Supplied Instrument Name: Attune Acoustic Focusing Cytometer Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/ Seal AA3 Segmented Flow Injection Autoanalyzer Seal AA3 Segmented Flow Injection Autoanalyzer PI Supplied Instrument Name: Seal AA3 Segmented Flow Injection Autoanalyzer Instrument Name: Flow Injection Analyzer Instrument Short Name:FIA Instrument Description: An instrument that performs flow injection analysis. Flow injection analysis (FIA) is an approach to chemical analysis that is accomplished by injecting a plug of sample into a flowing carrier stream. FIA is an automated method in which a sample is injected into a continuous flow of a carrier solution that mixes with other continuously flowing solutions before reaching a detector. Precision is dramatically increased when FIA is used instead of manual injections and as a result very specific FIA systems have been developed for a wide array of analytical techniques. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB36/ Horiba Aqualog scanning fluorometer Horiba Aqualog scanning fluorometer PI Supplied Instrument Name: Horiba Aqualog scanning fluorometer Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/ Thermo Finnigan DeltaPlus Isotope-Ratio mass spectrometer Thermo Finnigan DeltaPlus Isotope-Ratio mass spectrometer PI Supplied Instrument Name: Thermo Finnigan DeltaPlus Isotope-Ratio mass spectrometer Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/ Shimadzu TOC-V Shimadzu TOC-V PI Supplied Instrument Name: Shimadzu TOC-V Instrument Name: Shimadzu TOC-V Analyzer Instrument Short Name:Shimadzu TOC-V Instrument Description: A Shimadzu TOC-V Analyzer measures DOC by high temperature combustion method. Community Standard Description: http://onto.nerc.ac.uk/CAST/124 YSI Professional Plus YSI Professional Plus PI Supplied Instrument Name: YSI Professional Plus Instrument Name: YSI Professional Plus Multi-Parameter Probe Instrument Short Name:YSI ProPlus Instrument Description: The YSI Professional Plus handheld multiparameter meter provides for the measurement of a variety of combinations for dissolved oxygen, conductivity, specific conductance, salinity, resistivity, total dissolved solids (TDS), pH, ORP, pH/ORP combination, ammonium (ammonia), nitrate, chloride and temperature. More information from the manufacturer.