http://lod.bco-dmo.org/id/dataset/943333 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2024-11-12 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Specific daily growth of heterotrophic bacteria and grazing mortality on bacteria by microzooplankton from R/V Roger Revelle KIWI6, KIWI7, KIWI8, KIWI9 cruises in the Southern Ocean, 1997-1998 (U.S. JGOFS AESOPS project) 2024-11-14 publication 2024-11-14 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2024-11-14 publication https://doi.org/10.26008/1912/bco-dmo.943333.1 Michael R. Landry University of Hawaiʻi at Mānoa principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Landry, M. R. (2024) Specific daily growth of heterotrophic bacteria and grazing mortality on bacteria by microzooplankton from R/V Roger Revelle KIWI6, KIWI7, KIWI8, KIWI9 cruises in the Southern Ocean, 1997-1998 (U.S. JGOFS AESOPS project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-11-14 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.943333.1 [access date] Bacterivory Dataset Description: Methods and Sampling: &lt;p&gt;Rates of microzooplankton grazing were determined from serial dilution experiments as described in Landry et al. (2002). Seawater for most experiments was collected in 30-liter Go-Flo bottles, except for a few stations sampled with CTD Niskin bottles. Generally, two experiments were run at each station – one with water collected from the depth of penetration of ~25% of surface PAR, the other varying from 10 to 58% PAR depending on relative mixed-layer versus euphotic zone depths.&lt;/p&gt; &lt;p&gt;For each experiment, ten 2-liter polycarbonate bottles were used to establish a nutrient-enriched dilution series consisting of replicated bottles with 22, 45, 65, 86, and 100% natural (unfiltered) seawater with final concentrations of 0.5 micromolar (µM) ammonium, 0.03 µM phosphate, 1.0 nM FeSO4, and 0.1 nM MnSO4. Five additional bottles were filled with whole seawater with no nutrient enrichment. Two were used for initial samples, and the final three were incubated as natural seawater controls. All bottles were tightly capped after filling and incubated for ~48 hours in seawater-cooled incubators calibrated to the relative PAR light levels.&lt;/p&gt; Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-9634053 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=9634053 completed Michael R. Landry University of Hawaiʻi at Mānoa 858-534-4702 9500 Gilman Drive Mail Code: 0227 La Jolla CA 92093-0227 USA mlandry@ucsd.edu pointOfContact asNeeded Dataset Version: 1 Unknown Cruise Event Date Latitude Longitude Station Cast Cast_type Depth Bact_growth Microzoo_graz Coulter EPICS 753 flow cytometer 30-liter Go-Flo bottles CTD Niskin bottles theme None, User defined Cruise identfier event date latitude longitude station cast cast type depth growth featureType BCO-DMO Standard Parameters Flow Cytometer GO-FLO Bottle Niskin bottle instrument BCO-DMO Standard Instruments KIWI6 KIWI7 KIWI8 KIWI9 service Deployment Activity U.S. JGOFS Southern Ocean place Locations otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. U.S. Joint Global Ocean Flux Study http://usjgofs.whoi.edu/ U.S. Joint Global Ocean Flux Study The United States Joint Global Ocean Flux Study was a national component of international JGOFS and an integral part of global climate change research. The U.S. launched the Joint Global Ocean Flux Study (JGOFS) in the late 1980s to study the ocean carbon cycle. An ambitious goal was set to understand the controls on the concentrations and fluxes of carbon and associated nutrients in the ocean. A new field of ocean biogeochemistry emerged with an emphasis on quality measurements of carbon system parameters and interdisciplinary field studies of the biological, chemical and physical process which control the ocean carbon cycle. As we studied ocean biogeochemistry, we learned that our simple views of carbon uptake and transport were severely limited, and a new "wave" of ocean science was born. U.S. JGOFS has been supported primarily by the U.S. National Science Foundation in collaboration with the National Oceanic and Atmospheric Administration, the National Aeronautics and Space Administration, the Department of Energy and the Office of Naval Research. U.S. JGOFS, ended in 2005 with the conclusion of the Synthesis and Modeling Project (SMP). U.S. JGOFS largerWorkCitation program U.S. JGOFS Antarctic Environment and Southern Ocean Process Study http://usjgofs.whoi.edu/research/aesops.html U.S. JGOFS Antarctic Environment and Southern Ocean Process Study <p>The U.S. Southern Ocean JGOFS program, called Antarctic Environment and Southern Ocean Process Study (AESOPS), began in August 1996 and continued through March 1998. The U.S. JGOFS AESOPS program focused on two regions in the Southern Ocean: an east/west section of the Ross-Sea continental shelf along 76.5°S, and a second north/south section of the Southern Ocean spanning the Antarctic Circumpolar Current (ACC) at ~170°W (identified as the Polar Front). The science program, coordinated by Antarctic Support Associates (ASA), comprised eleven cruises using the R.V.I.B Nathaniel B. Palmer and R/V Roger Revelle as observational platforms and for deployment and recovery of instrumented moorings and sediment-trap arrays. The Ross-Sea region was occupied on six occasions and the Polar Front five times. Mapping data were obtained from SeaSoar, ADCP, and bathymetric systems. Satellite coverage was provided by the NASA SeaWiFS and the NOAA/NASA Pathfinder programs.</p> AESOPS largerWorkCitation project eng; USA oceans U.S. JGOFS Southern Ocean -174.73 -165.92 -70.4 -52.97 1997-10-24 1998-03-10 Southern Ocean, Ross Sea 0 BCO-DMO catalogue of parameters from Specific daily growth of heterotrophic bacteria and grazing mortality on bacteria by microzooplankton from R/V Roger Revelle KIWI6, KIWI7, KIWI8, KIWI9 cruises in the Southern Ocean, 1997-1998 (U.S. JGOFS AESOPS project) Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/943402.rdf Name: Cruise Units: unitless Description: <p>cruise ID (KIWI6, KIWI7, KIWI8 or KIWI9)</p> http://lod.bco-dmo.org/id/dataset-parameter/943403.rdf Name: Event Units: unitless Description: <p>unique event number in UTC time (MMDDHHmm) assigned to each sampling activity</p> http://lod.bco-dmo.org/id/dataset-parameter/943404.rdf Name: Date Units: unitless Description: <p>date (GMT)</p> http://lod.bco-dmo.org/id/dataset-parameter/943405.rdf Name: Latitude Units: decimal degrees Description: <p>latitude (North is positive; South is negative)</p> http://lod.bco-dmo.org/id/dataset-parameter/943406.rdf Name: Longitude Units: decimal degrees Description: <p>longitude (East is positive; West is negative)</p> http://lod.bco-dmo.org/id/dataset-parameter/943407.rdf Name: Station Units: unitless Description: <p>designated cruise sampling station</p> http://lod.bco-dmo.org/id/dataset-parameter/943408.rdf Name: Cast Units: unitless Description: <p>designated cast number at station</p> http://lod.bco-dmo.org/id/dataset-parameter/943409.rdf Name: Cast_type Units: unitless Description: <p>CTD=CTD rosette; TM=Trace Metal rosette</p> http://lod.bco-dmo.org/id/dataset-parameter/943410.rdf Name: Depth Units: meters (m) Description: <p>water sample depth</p> http://lod.bco-dmo.org/id/dataset-parameter/943411.rdf Name: Bact_growth Units: per day (d-1) Description: <p>growth rate of heterotrophic bacteria cells</p> http://lod.bco-dmo.org/id/dataset-parameter/943412.rdf Name: Microzoo_graz Units: per day (d-1) Description: <p>grazing rate of microzooplankton on bacteria</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 3242 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/c10b14f7-353b-4341-8189-4fb31c148bc8/content download https://doi.org/10.26008/1912/bco-dmo.943333.1 download onLine dataset &lt;p&gt;Rates of microzooplankton grazing were determined from serial dilution experiments as described in Landry et al. (2002). Seawater for most experiments was collected in 30-liter Go-Flo bottles, except for a few stations sampled with CTD Niskin bottles. Generally, two experiments were run at each station – one with water collected from the depth of penetration of ~25% of surface PAR, the other varying from 10 to 58% PAR depending on relative mixed-layer versus euphotic zone depths.&lt;/p&gt; &lt;p&gt;For each experiment, ten 2-liter polycarbonate bottles were used to establish a nutrient-enriched dilution series consisting of replicated bottles with 22, 45, 65, 86, and 100% natural (unfiltered) seawater with final concentrations of 0.5 micromolar (µM) ammonium, 0.03 µM phosphate, 1.0 nM FeSO4, and 0.1 nM MnSO4. Five additional bottles were filled with whole seawater with no nutrient enrichment. Two were used for initial samples, and the final three were incubated as natural seawater controls. All bottles were tightly capped after filling and incubated for ~48 hours in seawater-cooled incubators calibrated to the relative PAR light levels.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;Initial and final concentrations of bacterial cells were determined from 1-milliliter (mL) samples preserved with 0.5% paraformaldehyde, frozen in liquid nitrogen, and analyzed with a Coulter EPICS 753 flow cytometer with two lasers (UV and 488 nanometers (nm)) after Hoechst 33342 staining. Net rates of change (ki, d-1) during the incubations were computed from initial and final cell counts in each bottle; k = ln(Pf/Pi)/t, where t = 2 days. Mortality rates due to microzooplankton grazing (m, d-1) were computed as the slopes of the linear regressions between kiand Di for the nutrient-amended bottles, and growth rates (µ, d-1) were determined from net growth in bottles without added nutrients (Landry and Hassett, 1982).&lt;/p&gt; Specified by the Principal Investigator(s) - Imported file &quot;AESOPS_bacterivory_BCO_DMO submission 5Nov2024.csv&quot; into the BCO-DMO system. - Converted Date column to YYYY-MM-DD format. - Renamed fields to comply with BCO-DMO naming conventions. - Saved final file as &quot;943333_v1_bacterivory_aesops.csv&quot;. Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Coulter EPICS 753 flow cytometer Coulter EPICS 753 flow cytometer PI Supplied Instrument Name: Coulter EPICS 753 flow cytometer PI Supplied Instrument Description:Coulter EPICS 753 flow cytometer with two lasers (UV and 488 nm) Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/ 30-liter Go-Flo bottles 30-liter Go-Flo bottles PI Supplied Instrument Name: 30-liter Go-Flo bottles PI Supplied Instrument Description:Seawater for most experiments was collected in 30-liter Go-Flo bottles, except for a few stations sampled with CTD Niskin bottles. Instrument Name: GO-FLO Bottle Instrument Short Name:GO-FLO Instrument Description: GO-FLO bottle cast used to collect water samples for pigment, nutrient, plankton, etc. The GO-FLO sampling bottle is specially designed to avoid sample contamination at the surface, internal spring contamination, loss of sample on deck (internal seals), and exchange of water from different depths. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/30/ CTD Niskin bottles CTD Niskin bottles PI Supplied Instrument Name: CTD Niskin bottles PI Supplied Instrument Description:Seawater for most experiments was collected in 30-liter Go-Flo bottles, except for a few stations sampled with CTD Niskin bottles. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/ Cruise: KIWI6 KIWI6 R/V Roger Revelle Community Standard Description International Council for the Exploration of the Sea R/V Roger Revelle vessel KIWI6 Timothy Cowles Oregon State University http://usjgofs.whoi.edu/aesops/RRs1.html Report describing KIWI6 Cruise: KIWI7 KIWI7 R/V Roger Revelle Community Standard Description International Council for the Exploration of the Sea R/V Roger Revelle vessel KIWI7 Richard Barber Duke University http://usjgofs.whoi.edu/aesops/RRp1.html Report describing KIWI7 Cruise: KIWI8 KIWI8 R/V Roger Revelle Community Standard Description International Council for the Exploration of the Sea R/V Roger Revelle vessel KIWI8 Kenneth H. Coale Moss Landing Marine Laboratories http://usjgofs.whoi.edu/aesops/RRs2.html Report describing KIWI8 Cruise: KIWI9 KIWI9 R/V Roger Revelle Community Standard Description International Council for the Exploration of the Sea R/V Roger Revelle vessel KIWI9 Wilford D. Gardner Texas A&M University http://usjgofs.whoi.edu/aesops/RRp2.html Report describing KIWI9 R/V Roger Revelle Community Standard Description International Council for the Exploration of the Sea R/V Roger Revelle vessel