http://lod.bco-dmo.org/id/dataset/944892
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-12-03
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Protist carbon from microscopy samples collected in the Argo Basin of the Eastern Indian Ocean on R/V Roger Revelle cruise RR2202 from Feb to Mar 2022
2024-12-03
publication
2024-12-03
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-12-09
publication
https://doi.org/10.26008/1912/bco-dmo.944892.1
Michael R. Landry
University of California-San Diego Scripps
principalInvestigator
Michael R. Stukel
Florida State University
principalInvestigator
Natalia Yingling
Florida State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Yingling, N., Landry, M. R., Stukel, M. R. (2024) Protist carbon from microscopy samples collected in the Argo Basin of the Eastern Indian Ocean on R/V Roger Revelle cruise RR2202 from Feb to Mar 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-12-03 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.944892.1 [access date]
Protist carbon from microscopy samples collected in the Argo Basin of the Eastern Indian Ocean on R/V Roger Revelle cruise RR2202 from Feb to Mar 2022 Dataset Description: Methods and Sampling: <p><strong>Field Collection</strong></p>
<p>We conducted four Lagrangian experiments (hereafter referred to as “cycles”) that lasted three to five days. &nbsp;The mixed layer was followed in a Lagrangian frame of reference using subsurface drogues attached to satellite-tagged marker buoys&nbsp;(Landry et al. 2009; Stukel et al. 2015).&nbsp;Six depths were chosen to span the euphotic zone (based on chlorophyll fluorescence measured during the conductivity-temperature-depth (CTD) downcast profiles).We used a 12-place 6-L Niskin bottle rosette equipped with a Seabird Conductivity, Temperature, and Depth (CTD) sensor and profiling chlorophyll fluorometer to sample water daily at six depths spanning the euphotic zone for phytoplankton community samples.</p>
<p>The RR2201 cruise report can be found at <a href="http://hdl.handle.net/1834/43464" target="_blank">http://hdl.handle.net/1834/43464</a>.</p>
<p><strong>Epifluorescence Microscopy Sampling</strong></p>
<p>From each depth, two different volumes of water were sampled: 50 mL for nanoplankton- epifluorescence microscopy (filtered through a 0.8-µm pore-size black polycarbonate filter) and 400 mL for microplankton epifluorescence microscopy (filtered through an 8-µm pore-size filter). Utilizing two different sized filters and sampling volumes allowed for appropriate, adjustable filtered volumes and avoid overlapping cells on the slides.&nbsp;20&nbsp;µm&nbsp;backing filters were utilized as data has indicated that they support the membrane filters and ensure even dispersal of sample on the filter&nbsp;(Kemp&nbsp;et. al., 1993; Taylor et al., 2015).&nbsp;The samples were preserved&nbsp;using buffered formalin, alkaline Lugol’s solution, and sodium thiosulfate then stained using proflavine and 4’, 6-diamidino-2phenylindole (DAPI) (modified protocol from Sherr and Sherr, 1993 in&nbsp;Kemp et. al., 1993). During and immediately after filtration, filters were covered with tin foil to prevent photochemical quenching. Filters were mounted onto a glass slide and frozen in a -80º C freezer for later analysis.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1851381 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1851381
completed
Michael R. Landry
University of California-San Diego Scripps
858-534-4702
9500 Gilman Drive Mail Code: 0227
La Jolla
CA
92093-0227
USA
mlandry@ucsd.edu
pointOfContact
Michael R. Stukel
Florida State University
815-258-3875
Earth, Ocean, and Atmos. Science Building (EOA) Rm. 6089 1011 Academic Way
Tallahassee
FL
32306
USA
mstukel@fsu.edu
pointOfContact
Natalia Yingling
Florida State University
1011 Academic Way
Tallahassee
FL
USA
ny18b@fsu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cruise
Station_Event
DateTimeUTC
Date
Latitude
Longitude
Cycle
Day_Cycle
CTD_Cast
Depth
Autotrophic_nanoplankton_abundance
Autotrophic_nanoplankton_biomass
Heterotrophic_nanoplankton_abundance
Heterotrophic_nanoplankton_biomass
Autotrophic_microplankton_abundance
Autotrophic_microplankton_biomass
Heterotrophic_microplankton_abundance
Heterotrophic_microplankton_biomass
Olympus Microscope DP72 Camera
Seabird Conductivity, Temperature, and Depth (CTD) sensor
subsurface drogues attached to satellite-tagged marker buoys
Olympus BX51 microscope with a Olympus DP72 camera
profiling chlorophyll fluorometer
6-L Niskin bottle
theme
None, User defined
Cruise identfier
station
ISO_DateTime_UTC
date
latitude
longitude
experiment id
cast
depth
abundance
wet_weight_biomass
featureType
BCO-DMO Standard Parameters
Camera
CTD Sea-Bird 911
Drifter Buoy
Fluorescence Microscope
Fluorometer
Niskin bottle
instrument
BCO-DMO Standard Instruments
RR2201
service
Deployment Activity
Eastern Indian Ocean (Argo Basin)
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Second International Indian Ocean Expedition
https://web.whoi.edu/iioe2/
Second International Indian Ocean Expedition
Description from the program website:
The Second International Indian Ocean Expedition (IIOE-2) is a major global scientific program which will engage the international scientific community in collaborative oceanographic and atmospheric research from coastal environments to the deep sea over the period 2015-2020, revealing new information on the Indian Ocean (i.e. its currents, its influence upon the climate, its marine ecosystems) which is fundamental for future sustainable development and expansion of the Indian Ocean's blue economy. A large number of scientists from research institutions from around the Indian Ocean and beyond are planning their involvement in IIOE-2 in accordance with the overarching six scientific themes of the program. Already some large collaborative research projects are under development, and it is anticipated that by the time these projects are underway, many more will be in planning or about to commence as the scope and global engagement in IIOE-2 grows.
Focused research on the Indian Ocean has a number of benefits for all nations. The Indian Ocean is complex and drives the region's climate including extreme events (e.g. cyclones, droughts, severe rains, waves and storm surges). It is the source of important socio-economic resources (e.g. fisheries, oil and gas exploration/extraction, eco-tourism, and food and energy security) and is the background and focus of many of the region's human populations around its margins. Research and observations supported through IIOE-2 will result in an improved understanding of the ocean's physical and biological oceanography, and related air-ocean climate interactions (both in the short-term and long-term). The IIOE-2's program will complement and harmonise with other regional programs underway and collectively the outcomes of IIOE-2 will be of huge benefit to individual and regional sustainable development as the information is a critical component of improved decision making in areas such as maritime services and safety, environmental management, climate monitoring and prediction, food and energy security.
IIOE-2 activities will also include a significant focus on building the capacity of all nations around the Indian Ocean to understand and apply observational data or research outputs for their own socio-economic requirements and decisions. IIOE-2 capacity building programs will therefore be focused on the translation of the science and information outputs for societal benefit and training of relevant individuals from surrounding nations in these areas.
A Steering Committee was established to support U.S. participation in IIOE-2. More information is available on their website at https://web.whoi.edu/iioe2/.
IIOE-2
largerWorkCitation
program
Collaborative Research: Mesoscale variability in nitrogen sources and food-web dynamics supporting larval southern bluefin tuna in the eastern Indian Ocean
https://osprey.bco-dmo.org/project/819488
Collaborative Research: Mesoscale variability in nitrogen sources and food-web dynamics supporting larval southern bluefin tuna in the eastern Indian Ocean
<p><em>NSF Award Abstract:</em><br />
The small area between NW Australia and Indonesia in the eastern Indian Ocean (IO) is the only known spawning ground of Southern Bluefin Tuna (SBT), a critically endangered top marine predator. Adult SBT migrate thousands of miles each year from high latitude feeding areas to lay their eggs in these tropical waters, where food concentrations on average are below levels that can support optimal feeding and growth of their larvae. Many critical aspects of this habitat are poorly known, such as the main source of nitrogen nutrient that sustains system productivity, how the planktonic food web operates to produce the unusual types of zooplankton prey that tuna larvae prefer, and how environmental differences in habitat quality associated with ocean fronts and eddies might be utilized by adult spawning tuna to give their larvae a greater chance for rapid growth and survival success. This project investigates these questions on a 38-day expedition in early 2021, during the peak time of SBT spawning. This project is a US contribution to the 2nd International Indian Ocean Expedition (IIOE-2) that advances understanding of biogeochemical and ecological dynamics in the poorly studied eastern IO. This is the first detailed study of nitrogen and carbon cycling in the region linking Pacific and IO waters. The shared dietary preferences of SBT larvae with those of other large tuna and billfish species may also make the insights gained broadly applicable to understanding larval recruitment issues for top consumers in other marine ecosystems. New information from the study will enhance international management efforts for SBT. The shared larval dietary preferences of large tuna and billfish species may also extend the insights gained broadly to many other marine top consumers, including Atlantic bluefin tuna that spawn in US waters of the Gulf of Mexico. The end-to-end study approach, highlights connections among physical environmental variability, biogeochemistry, and plankton food webs leading to charismatic and economically valuable fish production, is the theme for developing educational tools and modules through the "scientists-in-the-schools" program of the Center for Ocean-Atmospheric Prediction Studies at Florida State University, through a program for enhancing STEM learning pathways for underrepresented students in Hawaii, and through public outreach products for display at the Birch Aquarium in San Diego. The study also aims to support an immersive field experience to introduce talented high school students to marine research, with the goal of developing a sustainable marine-related educational program for underrepresented students in rural northwestern Florida.</p>
<p>Southern Bluefin Tuna (SBT) migrate long distances from high-latitude feeding grounds to spawn exclusively in a small oligotrophic area of the tropical eastern Indian Ocean (IO) that is rich in mesoscale structures, driven by complex currents and seasonally reversing monsoonal winds. To survive, SBT larvae must feed and grow rapidly under environmental conditions that challenge conventional understanding of food-web structure and functional relationships in poor open-ocean systems. The preferred prey of SBT larvae, cladocerans and Corycaeidae copepods, are poorly studied and have widely different implications for trophic transfer efficiencies to larvae. Differences in nitrogen sources - N fixation vs deep nitrate of Pacific origin - to sustain new production in the region also has implications for conditions that may select for prey types (notably cladocerans) that enhance transfer efficiency and growth rates of SBT larvae. The relative importance of these N sources for the IO ecosystem may affect SBT resiliency to projected increased ocean stratification. This research expedition investigates how mesoscale variability in new production, food-web structure and trophic fluxes affects feeding and growth conditions for SBT larvae. Sampling across mesoscale features tests hypothesized relationships linking variability in SBT larval feeding and prey preferences (gut contents), growth rates (otolith analyses) and trophic positions (TP) to the environmental conditions of waters selected by adult spawners. Trophic Positions of larvae and their prey are determined using Compound-Specific Isotope Analyses of Amino Acids (CSIA-AA). Lagrangian experiments investigate underlying process rates and relationships through measurements of water-column 14C productivity, N2 fixation, 15NO3- uptake and nitrification; community biomass and composition (flow cytometry, pigments, microscopy, in situ imaging, genetic analyses); and trophic fluxes through micro- and mesozooplankton grazing, remineralization and export. Biogeochemical and food web elements of the study are linked by CSIA-AA (N source, TP), 15N-constrained budgets and modeling. The project elements comprise an end-to-end coupled biogeochemistry-trophic study as has not been done previously for any pelagic ecosystem.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
BLOOFINZ-IO
largerWorkCitation
project
eng; USA
oceans
Eastern Indian Ocean (Argo Basin)
114.1351
118.1424
-17.1263
-15.3494
2022-02-03
2022-02-22
Eastern Indian Ocean, Indonesian Throughflow area, and the Gulf of Mexico
0
BCO-DMO catalogue of parameters from Protist carbon from microscopy samples collected in the Argo Basin of the Eastern Indian Ocean on R/V Roger Revelle cruise RR2202 from Feb to Mar 2022
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/945058.rdf
Name: Cruise
Units: unitless
Description: <p>R/V Revelle cruise ID number</p>
http://lod.bco-dmo.org/id/dataset-parameter/945059.rdf
Name: Station_Event
Units: unitless
Description: <p>Station designation incorporating date and UTC time</p>
http://lod.bco-dmo.org/id/dataset-parameter/945060.rdf
Name: DateTimeUTC
Units: unitless
Description: <p>Date and time (UTC) in ISO 8601 format of collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/945061.rdf
Name: Date
Units: unitless
Description: <p>Date of collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/945062.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude; negative values = South</p>
http://lod.bco-dmo.org/id/dataset-parameter/945063.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude; positive values = East</p>
http://lod.bco-dmo.org/id/dataset-parameter/945064.rdf
Name: Cycle
Units: unitless
Description: <p>Lagrangian experiment number</p>
http://lod.bco-dmo.org/id/dataset-parameter/945065.rdf
Name: Day_Cycle
Units: unitless
Description: <p>Day of Lagrangian cycle</p>
http://lod.bco-dmo.org/id/dataset-parameter/945066.rdf
Name: CTD_Cast
Units: unitless
Description: <p>Sequential CTD number on the cruise</p>
http://lod.bco-dmo.org/id/dataset-parameter/945067.rdf
Name: Depth
Units: meters (m)
Description: <p>Depth of water collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/945068.rdf
Name: Autotrophic_nanoplankton_abundance
Units: cells/mL
Description: <p>The number of autotrophic nanoplankton (2 to 20 µm in size)</p>
http://lod.bco-dmo.org/id/dataset-parameter/945069.rdf
Name: Autotrophic_nanoplankton_biomass
Units: µg C L-1 (micrograms of carbon per liter)
Description: <p>The biomass of autotrophic nanoplankton (2 to 20 µm in size)</p>
http://lod.bco-dmo.org/id/dataset-parameter/945070.rdf
Name: Heterotrophic_nanoplankton_abundance
Units: cells/mL
Description: <p>The number of heterotrophic nanoplankton (2 to 20 µm in size)</p>
http://lod.bco-dmo.org/id/dataset-parameter/945071.rdf
Name: Heterotrophic_nanoplankton_biomass
Units: µg C L-1 (micrograms of carbon per liter)
Description: <p>The biomass of heterotrophic nanoplankton (2 to 20 µm in size)</p>
http://lod.bco-dmo.org/id/dataset-parameter/945072.rdf
Name: Autotrophic_microplankton_abundance
Units: cells/mL
Description: <p>The number of autotrophic microplankton (>20 µm in size)</p>
http://lod.bco-dmo.org/id/dataset-parameter/945073.rdf
Name: Autotrophic_microplankton_biomass
Units: µg C L-1 (micrograms of carbon per liter)
Description: <p>The biomass of autotrophic microplankton (>20µm in size)</p>
http://lod.bco-dmo.org/id/dataset-parameter/945074.rdf
Name: Heterotrophic_microplankton_abundance
Units: cells/mL
Description: <p>The number of heterotrophic microplankton (>20 µm in size)</p>
http://lod.bco-dmo.org/id/dataset-parameter/945075.rdf
Name: Heterotrophic_microplankton_biomass
Units: µg C L-1 (micrograms of carbon per liter)
Description: <p>The biomass of heterotrophic microplankton (>20µm in size)</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
9867
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/1c68123d-e046-4ad8-a0c1-27aecb220738/content
download
https://doi.org/10.26008/1912/bco-dmo.944892.1
download
onLine
dataset
<p><strong>Field Collection</strong></p>
<p>We conducted four Lagrangian experiments (hereafter referred to as “cycles”) that lasted three to five days. &nbsp;The mixed layer was followed in a Lagrangian frame of reference using subsurface drogues attached to satellite-tagged marker buoys&nbsp;(Landry et al. 2009; Stukel et al. 2015).&nbsp;Six depths were chosen to span the euphotic zone (based on chlorophyll fluorescence measured during the conductivity-temperature-depth (CTD) downcast profiles).We used a 12-place 6-L Niskin bottle rosette equipped with a Seabird Conductivity, Temperature, and Depth (CTD) sensor and profiling chlorophyll fluorometer to sample water daily at six depths spanning the euphotic zone for phytoplankton community samples.</p>
<p>The RR2201 cruise report can be found at <a href="http://hdl.handle.net/1834/43464" target="_blank">http://hdl.handle.net/1834/43464</a>.</p>
<p><strong>Epifluorescence Microscopy Sampling</strong></p>
<p>From each depth, two different volumes of water were sampled: 50 mL for nanoplankton- epifluorescence microscopy (filtered through a 0.8-µm pore-size black polycarbonate filter) and 400 mL for microplankton epifluorescence microscopy (filtered through an 8-µm pore-size filter). Utilizing two different sized filters and sampling volumes allowed for appropriate, adjustable filtered volumes and avoid overlapping cells on the slides.&nbsp;20&nbsp;µm&nbsp;backing filters were utilized as data has indicated that they support the membrane filters and ensure even dispersal of sample on the filter&nbsp;(Kemp&nbsp;et. al., 1993; Taylor et al., 2015).&nbsp;The samples were preserved&nbsp;using buffered formalin, alkaline Lugol’s solution, and sodium thiosulfate then stained using proflavine and 4’, 6-diamidino-2phenylindole (DAPI) (modified protocol from Sherr and Sherr, 1993 in&nbsp;Kemp et. al., 1993). During and immediately after filtration, filters were covered with tin foil to prevent photochemical quenching. Filters were mounted onto a glass slide and frozen in a -80º C freezer for later analysis.</p>
Specified by the Principal Investigator(s)
<p>Slides were imaged using an Olympus Microscope DP72 Camera on an Olympus BX51 fluorescence microscope.&nbsp;Slides were calibrated from pixels to microns for both 0.8 µm and 8 µm to determine what length of pixels equates to microns.&nbsp;Our data includes conversion ratios (0.0012 for 0.8 µm and 0.0072 for 8 µm), which should be applied when determining biomass or abundance, along with the volume filtered. These ratios account for the area of the images taken and represent the proportion of cells actually counted for the 0.8 µm and 8 µm samples, and they have already been incorporated into the dataset presented.</p>
<p>Twenty 8-µm images (20x objective lens) and thirty 0.8-µm images (60x objective lens) were captured using filter sets that were suited for proflavine (green fluorescence with 482 nm excitation, 536 nm emission filters), auto-chlorophyll fluorescence (red fluorescence with 450-490 nm excitation, 660-680 nm emission) and DAPI (blue fluorescence with 350 nm excitation, 465 nm emission) to capture the fluorescence of cell proteins, chlorophyll and DNA. The images were subsequently analyzed to identify phototrophs and heterotrophs, with cells marked based on the presence of both fluorescence signals (indicating phototrophs) or only proflavine fluorescence (indicating heterotrophs).ImageJ image analysis software (v 1.52a or 1.53c)&nbsp;was utilized for processing images, involving manually outlining cells in order to calculate feret length, area and width. Cells that were &gt;50% out of frame or broken were not included in analyses. Images were calibrated to microns and a conversion factor was applied to include the number of images, area of the slide and area imaged with the specific camera/lens combination.&nbsp;</p>
<p>To determine the true filtration diameter, a light microscope was used to examine a 25 mm glass fiber filter (GF/F) filter that had a small amount of dyed water filtered through. It was discovered that the filter funnel blocks roughly 12% of the 25-mm filter and the filtered region had a diameter of 22 mm. Equations 1-5 show the equations used to calculate cell width, biovolume, ESD (equivalent spherical diameter) and biomass, where * implies we assumed that cell height = cell width/2. ESD was used as a consistent measure of mean cell size since many plankton have an irregular shape. The height of a cell was assumed to be roughly equivalent to half of the cell width since cells are often flattened during filtration&nbsp;(Taylor et al., 2011)&nbsp;with the exception of diatoms. The biomass of diatoms (which were the only taxon we could conclusively identify) was estimated allometrically&nbsp;using equation 5 while all other cell biomass (non-diatoms) was estimated allometrically using equation 4.&nbsp;(Menden-Deuer and Lessard, 2000).&nbsp;</p>
<p>&nbsp;</p>
<p>Equation 1: Cell width = (4/π) x (Area of the cell/Feret length of the cell)</p>
<p>Equation 2: Biovolume = (4/3)(π) x (Feret Length/2) x (Cell Width/2) x (Cell Height*/2)</p>
<p>Equation 3: ESD = 2 x (3 x Biovolume/4π)^(1/3)</p>
<p>Equation 4: Biomass (non-diatoms) = 0.216 x Biovolume^0.939</p>
<p>Equation 5: Biomass (diatoms) = 0.288 x Biovolume^0.811</p>
Specified by the Principal Investigator(s)
-imported "BF_IO_Epifluroescence_Microscopy_Carbon_Data.xlsx" without excel formatting into BCO-DMO system
- split "Cycle,Day" column on commas into "Cycle" and "Day_Cycle"
- removed T00:00:00 from "Date" field values
- rounded biomass fields to 3 places
- renamed fields for BCO-DMO system requirements and clarity
- exported "944892_v1_protist_carbon.csv"
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Olympus Microscope DP72 Camera
Olympus Microscope DP72 Camera
PI Supplied Instrument Name: Olympus Microscope DP72 Camera PI Supplied Instrument Description:Slides were imaged using an Olympus Microscope DP72 Camera on an Olympus BX51 fluorescence microscope. Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/
Seabird Conductivity, Temperature, and Depth (CTD) sensor
Seabird Conductivity, Temperature, and Depth (CTD) sensor
PI Supplied Instrument Name: Seabird Conductivity, Temperature, and Depth (CTD) sensor PI Supplied Instrument Description:We used a 12-place 6-L Niskin bottle rosette equipped with a Seabird Conductivity, Temperature, and Depth (CTD) sensor and profiling chlorophyll fluorometer to sample water daily at six depths spanning the euphotic zone for phytoplankton community samples. Instrument Name: CTD Sea-Bird 911 Instrument Short Name:CTD SBE 911 Instrument Description: The Sea-Bird SBE 911 is a type of CTD instrument package. The SBE 911 includes the SBE 9 Underwater Unit and the SBE 11 Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). More information from Sea-Bird Electronics. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0035/
subsurface drogues attached to satellite-tagged marker buoys
subsurface drogues attached to satellite-tagged marker buoys
PI Supplied Instrument Name: subsurface drogues attached to satellite-tagged marker buoys PI Supplied Instrument Description:The mixed layer was followed in a Lagrangian frame of reference using subsurface drogues attached to satellite-tagged marker buoys (Landry et al. 2009; Stukel et al. 2015). Instrument Name: Drifter Buoy Instrument Short Name: Instrument Description: Drifting buoys are free drifting platforms with a float or buoy that keep the drifter at the surface and underwater sails or socks that catch the current. These instruments sit at the surface of the ocean and are transported via near-surface ocean currents. They are not fixed to the ocean bottom, therefore they "drift" with the currents. For this reason, these instruments are referred to as drifters, or drifting buoys.
The surface float contains sensors that measure different parameters, such as sea surface temperature, barometric pressure, salinity, wave height, etc. Data collected from these sensors are transmitted to satellites passing overhead, which are then relayed to land-based data centers.
definition sources: https://mmisw.org/ont/ioos/platform/drifting_buoy and https://www.aoml.noaa.gov/phod/gdp/faq.php#drifter1
Olympus BX51 microscope with a Olympus DP72 camera
Olympus BX51 microscope with a Olympus DP72 camera
PI Supplied Instrument Name: Olympus BX51 microscope with a Olympus DP72 camera PI Supplied Instrument Description:Epifluorescence microscope: Olympus BX51 microscope with a Olympus DP72 camera and Exfo X-cite Series 120 mercury bulb with a FITC filter for green fluorescence. Instrument Name: Fluorescence Microscope Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/
profiling chlorophyll fluorometer
profiling chlorophyll fluorometer
PI Supplied Instrument Name: profiling chlorophyll fluorometer PI Supplied Instrument Description:We used a 12-place 6-L Niskin bottle rosette equipped with a Seabird Conductivity, Temperature, and Depth (CTD) sensor and profiling chlorophyll fluorometer to sample water daily at six depths spanning the euphotic zone for phytoplankton community samples. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
6-L Niskin bottle
6-L Niskin bottle
PI Supplied Instrument Name: 6-L Niskin bottle PI Supplied Instrument Description:We used a 12-place 6-L Niskin bottle rosette equipped with a Seabird Conductivity, Temperature, and Depth (CTD) sensor and profiling chlorophyll fluorometer to sample water daily at six depths spanning the euphotic zone for phytoplankton community samples. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: RR2201
RR2201
R/V Roger Revelle
Community Standard Description
International Council for the Exploration of the Sea
R/V Roger Revelle
vessel
RR2201
Michael R. Landry
University of California-San Diego Scripps
http://hdl.handle.net/1834/43464
Report describing RR2201
R/V Roger Revelle
Community Standard Description
International Council for the Exploration of the Sea
R/V Roger Revelle
vessel