http://lod.bco-dmo.org/id/dataset/944935
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-12-03
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Hydrogen peroxide influence on toxicity of cyanobacterial harmful algal blooms (CHABs) in Lake Erie and other eutrophic waters from 2017 - 2019
2025-02-03
publication
2025-02-03
revision
BCO-DMO Linked Data URI
2025-02-03
creation
http://lod.bco-dmo.org/id/dataset/944935
Gregory J. Dick
University of Michigan
principalInvestigator
Rose Cory
University of Michigan
principalInvestigator
George Kling
University of Michigan
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Smith, D., Kling, G., Cory, R., Dick, G. J. (2025) Hydrogen peroxide influence on toxicity of cyanobacterial harmful algal blooms (CHABs) in Lake Erie and other eutrophic waters from 2017 - 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-02-03 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/944935 [access date]
Methods and Sampling: <p>Water samples were collected in the western basin of Lake Erie during the summer and fall of 2017, 2018, and 2019.&nbsp; In 2017, water was collected approximately weekly from NOAA station WE2 in conjunction with the NOAA Great Lakes Environmental Research Lab (GLERL) harmful algal bloom monitoring program.&nbsp; During August and October 2017, lake water was also collected by Environment and Climate Change Canada’s monitoring program.&nbsp; In 2018 and 2019, lake water was collected at several stages of bloom development (pre-bloom, early bloom, late bloom, and post bloom).&nbsp; In 2018, lake water was collected at NOAA’s monitoring stations WE2 and WE12 and at the drinking water intake for the City of Toledo (TWI).&nbsp; During summer 2019, the goal was to sample lake waters containing high bloom biomass as predicted by the NOAA HAB forecast model and HAB tracker bulletins (Wynne et al. 2013).&nbsp; Sampling sites were chosen based on the presence of surface scums comprised of dense cyanobacterial colonies (i.e., “bloom chase” sites).</p>
<p>For all sites, a depth-integrated water sample was collected in acid-washed carboys.&nbsp; Water samples were collected from the NOAA stations using a peristaltic pump.&nbsp; The pump hose was moved down the water column from the surface to 1 meter above the bottom.&nbsp; For the TWI, bloom chase, and Environment Canada cruise sites, a depth-integrated sample was collected by pooling water collected at 1 m intervals from surface to 1 meter above the lake bottom using a Niskin (Environment Canada) or Van Dorn (TWI and bloom chase sites) bottle.&nbsp; Integrated water samples were stored in carboys in an outdoor aquaculture tank until the start of the bottle experiments the following morning.&nbsp; The water temperature in the aquaculture tank was controlled using copper piping attached to a NESLAB RTE refrigerated water bath (Thermo Scientific, Newington, NH) and maintained at the lake temperature measured at the time of sample collection.&nbsp; During the Environment Canada cruises, bottles and carboys were stored in a plexiglass tank continuously circulated with fresh lake water.</p>
<p>Subsamples for supporting water quality analyses were taken from each carboy.&nbsp; Upon arrival in the laboratory at the University of Michigan, a subsample of whole (unfiltered) water was taken for analysis of total phosphorus.&nbsp; During 2017, pH of the water from each site was obtained from NOAA monitoring buoys.&nbsp; For samples collected in 2018-2019, pH of the whole water was measured upon arrival in the laboratory.&nbsp;&nbsp; Subsamples of the whole water were filtered through a 0.22 μm polyethersulfone (PES) filter for subsequent analysis of total dissolved phosphorus (TDP), soluble reactive phosphorous, nitrate and ammonium, dissolved organic carbon (DOC), and chromophoric and fluorescent dissolved organic matter (CDOM and FDOM, respectively).&nbsp; DOC samples were preserved by addition of 6N trace metal grade hydrochloric acid to pH 3.&nbsp;&nbsp; TDP, SRP, DOC, CDOM and FDOM were stored in the dark at 4 °C until analysis.&nbsp; Nitrate and ammonium samples were stored at -20 °C until analysis at GLERL.</p>
<p>Subsamples for H<sub>2</sub>O<sub>2</sub> and DNA were collected by filtering 100-200 mL of water from each bottle through a 0.22 μm pore size PES filter and collecting the last 50 mL of filtrate into a centrifuge tube.&nbsp; The filtered water for H<sub>2</sub>O<sub>2</sub> analysis was stored in the dark at 4 °C until analysis within 4 hours of collection.&nbsp; H<sub>2</sub>O<sub>2</sub> concentrations were measured on an FeLume by flow injection analysis using standard additions as previously described (King et al. 2007, as applied to Lake Erie waters in Cory et al. 2017; Pandey et al. 2022).&nbsp; The filter was saved for DNA extraction by freezing in a cryovial containing 1 mL RNAlater at -80 °C.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1736629 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1736629
onGoing
Gregory J. Dick
University of Michigan
734-763-3228
Department of Earth and Environmental Sciences 1100 N. University Ave., 2534 CC Little Building
Ann Arbor
MI
48109-1005
USA
gdick@umich.edu
pointOfContact
Rose Cory
University of Michigan
734-615-3199
Department of Earth and Environmental Sciences 2534 CC Little Bldg, 1100 N. University Ave
Ann Arbor
MI
48109-1005
USA
rmcory@umich.edu
pointOfContact
George Kling
University of Michigan
734-647-0898
Department of Ecology & Evolutionary Biology
Ann Arbor
MI
48109-1048
USA
gwk@umich.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Collection_Date
Experiment_Date
Incubation_Type
Site
Latitude
Longitude
Chlorophyll_a
Chlorophyll_a_95percent_CI
Dissolved_Inorganic_Carbon
Dissolved_Inorganic_Carbon_95percent_CI
H2CO3_star
H2CO3_star_95percent_CI
Bicarbonate
Bicarbonate_95percent_CI
Carbonate
Carbonate_95percent_CI
Dissolved_Organic_Carbon
Respiration_Rate
Respiration_Rate_95percent_CI
Primary_Production_Rate
Primary_Production_Rate_95percent_CI
CDOM_a305
CDOM_95percent_CI
Day_Integrated_UV_A
Day_Integrated_UV_B
Day_Integrated_UV
pH
Total_Phosphorus
Total_Phosphorus_95percent_CI
Total_Dissolved_Phosphorus
Total_Dissolved_Phosphorus_95percent_CI
Nitrate
Nitrate_95percent_CI
Ammonium
Ammonium_95percent_CI
Soluble_Reactive_Phosphorus
Soluble_Reactive_Phosphorus_95percent_CI
Average_Incubation_Temperature
Incubation_Temperature_Standard_Deviation
peak_A
peak_A_95percent_CI
peak_C
peak_C_95percent_CI
peak_T
peak_T_95percent_CI
peak_C_peak_A_ratio
peak_C_peak_A_ratio_95percent_CI
peak_T_peak_A_ratio
peak_T_peak_A_ratio_95percent_CI
Integrated_Fluorescence
Integrated_Fluorescence_95percent_CI
Fluorescence_Index
Fluorescence_Index_95percent_CI
Slope_Ratio
Slope_Ratio_95percent_CI
NESLAB RTE refrigerated water bath
FeLume
Aqualog fluorometer
peristaltic pump
theme
None, User defined
date
experiment type
site
latitude
longitude
chlorophyll a
dissolved inorganic Carbon
No BCO-DMO term
bicarbonate concentration [HCO3]-
Carbonate concentration [CO3]2-
dissolved organic Carbon
respiration rate
primary production
colored or chromophoric dissolved organic matter
pH
Phosphorus
Total Dissolved Phosphorus
Nitrate
Ammonium
SRP
incubation temperature
fDOM
fluorescence
featureType
BCO-DMO Standard Parameters
circulating water bath
Flow Injection Analyzer
Fluorometer
Niskin bottle
Pump
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The role of heterotrophic bacteria in protecting cyanobacteria from hydrogen peroxide in coastal ecosystems
https://osprey.bco-dmo.org/project/700767
The role of heterotrophic bacteria in protecting cyanobacteria from hydrogen peroxide in coastal ecosystems
<p><em>NSF Award Abstract:</em><br />
Toxic cyanobacterial harmful algal blooms (CHABs) are now a worldwide problem that poses dangers for humans and aquatic organisms including life-threatening sickness, beach closures, health alerts, and drinking water treatment plant closures. This project focuses on the basic science needed to understand interactions between the microorganisms present in CHABs and the chemistry of the lakes they inhabit. In particular, it will study the sources, fate, and effects of hydrogen peroxide, which is a potentially important control on the toxicity and species present within these blooms. This research will be conducted in Lake Erie, a source of drinking water for 11 million people that is threatened by CHABs annually. Results will be directly integrated into two water quality models that are widely used by water managers and other stakeholders. This project will support the training of two PhD students, including a first-generation college attendee, and undergraduate students from backgrounds that are underrepresented in the earth sciences. Research will also be integrated into outreach aimed at increasing diversity in the earth sciences by involving women and underrepresented minorities in K-12 as well as college and adult educational settings.</p>
<p>The overall goal of this project is to determine the influence of hydrogen peroxide (H2O2) on cyanobacterial community composition and function in nearshore ecosystems. Preliminary results from Lake Erie show that dominant primary producers rely on heterotrophic bacteria to draw down H2O2 from transiently high environmental levels that are likely inhibitory to members of the cyanobacterial community. This suggests that H2O2 plays important and still poorly understood roles in aquatic microbial ecology. A combination of field sampling, experiments, and state-of-the art "-omics" will be used to test the overall hypothesis that H2O2 decomposition by heterotrophic "helpers" is an important determinant of microbial interactions and community structure and function. Lake Erie will be studied because (i) it is a model system for shallow coastal areas receiving high terrestrial nutrient runoff, (ii) it offers strong inshore-offshore gradients of light and nutrients for comparative studies, and (iii) existing sampling infrastructure, archived samples, and preliminary data can be leveraged. Field and laboratory experiments and measurements will be integrated to answer the following questions: Q1: What drives the temporal dynamics of H2O2 concentrations? Q2: Which enzymes and organisms are responsible for protecting the community via biological H2O2 decay? Q3: How does protection from H2O2 by helpers influence the composition and function of the community? The study will perform controlled lab experiments on cultures and on natural waters during different points of the bloom. Measures of H2O2 concentrations and rates of production and decay, along with supporting chemical and biological measurements, will be used to assess the major sources and sinks of H2O2. Molecular tools will be used to determine the pathways underpinning H2O2 decay and the effect of H2O2 on cyanobacterial community composition function. In parallel, impacts of varying H2O2 concentrations on growth rates of major cyanobacteria will be assessed experimentally. These experimental results will be placed into context through comparisons with the structure and function of microbial communities from field samples across spatial, temporal, and chemical gradients in this coastal ecosystem. The approach of integrating studies of H2O2 with "-omics" in natural systems is novel, and will advance our fundamental knowledge and understanding of the relationship between microbial community composition and function.</p>
Lake Erie H2O2
largerWorkCitation
project
eng; USA
inlandWaters
2017-05-30
2019-09-19
Western Basin of Lake Erie (41N, 83W)
0
BCO-DMO catalogue of parameters from Hydrogen peroxide influence on toxicity of cyanobacterial harmful algal blooms (CHABs) in Lake Erie and other eutrophic waters from 2017 - 2019
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/950232.rdf
Name: Collection_Date
Units: unitless
Description: <p>Collection date</p>
http://lod.bco-dmo.org/id/dataset-parameter/950233.rdf
Name: Experiment_Date
Units: unitless
Description: <p>Experiment date</p>
http://lod.bco-dmo.org/id/dataset-parameter/950234.rdf
Name: Incubation_Type
Units: unitless
Description: <p>Incubation conditions</p>
http://lod.bco-dmo.org/id/dataset-parameter/950235.rdf
Name: Site
Units: unitless
Description: <p>Sampling site</p>
http://lod.bco-dmo.org/id/dataset-parameter/950236.rdf
Name: Latitude
Units: decimal degrees
Description: <p>latitude, South is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/950237.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude, West is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/950238.rdf
Name: Chlorophyll_a
Units: microgram/Liter (ug/L)
Description: <p>Chlorophyll a</p>
http://lod.bco-dmo.org/id/dataset-parameter/950239.rdf
Name: Chlorophyll_a_95percent_CI
Units: microgram/Liter (ug/L)
Description: <p>Chlorophyll a 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950240.rdf
Name: Dissolved_Inorganic_Carbon
Units: micrometer (uM)
Description: <p>dissolved inorganic Carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/950241.rdf
Name: Dissolved_Inorganic_Carbon_95percent_CI
Units: micrometer (uM)
Description: <p>Chlorophyll a 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950242.rdf
Name: H2CO3_star
Units: micrometer (uM)
Description: <p>H2CO3* (the total dissolved inorganic cabon including aqueous carbon dioxide)</p>
http://lod.bco-dmo.org/id/dataset-parameter/950243.rdf
Name: H2CO3_star_95percent_CI
Units: micrometer (uM)
Description: <p>H2CO3* 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950244.rdf
Name: Bicarbonate
Units: micrometer (uM)
Description: <p>Bicarbonate</p>
http://lod.bco-dmo.org/id/dataset-parameter/950245.rdf
Name: Bicarbonate_95percent_CI
Units: micrometer (uM)
Description: <p>Bicarbonate 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950246.rdf
Name: Carbonate
Units: micrometer (uM)
Description: <p>Carbonate</p>
http://lod.bco-dmo.org/id/dataset-parameter/950247.rdf
Name: Carbonate_95percent_CI
Units: micrometer (uM)
Description: <p>Carbonate 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950248.rdf
Name: Dissolved_Organic_Carbon
Units: micrometer (uM)
Description: <p>dissolved organic Carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/950249.rdf
Name: Respiration_Rate
Units: micrometer O2 / day (uM O2 / day)
Description: <p>Respiration Rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/950250.rdf
Name: Respiration_Rate_95percent_CI
Units: micrometer O2 / day (uM O2 / day)
Description: <p>Respiration Rate 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950251.rdf
Name: Primary_Production_Rate
Units: micrometer C / hour (uM C / hr)
Description: <p>Primary Production Rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/950252.rdf
Name: Primary_Production_Rate_95percent_CI
Units: micrometer C / hour (uM C / hr)
Description: <p>Primary Production Rate 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950253.rdf
Name: CDOM_a305
Units: per meter (m-1)
Description: <p>CDOM (a305)</p>
http://lod.bco-dmo.org/id/dataset-parameter/950254.rdf
Name: CDOM_95percent_CI
Units: per meter (m-1)
Description: <p>CDOM 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950255.rdf
Name: Day_Integrated_UV_A
Units: joules per meter squared (J/m^2)
Description: <p>total amount of UV-A that the experimental bottles were exposed to during the 9 hour experiments</p>
http://lod.bco-dmo.org/id/dataset-parameter/950256.rdf
Name: Day_Integrated_UV_B
Units: joules per meter squared (J/m^2)
Description: <p>total amount of UV-B that the experimental bottles were exposed to during the 9 hour experiments</p>
http://lod.bco-dmo.org/id/dataset-parameter/950257.rdf
Name: Day_Integrated_UV
Units: joules per meter squared (J/m^2)
Description: <p>sum of UV-A and UV-B that the experimental bottles were exposed to during the 9 hour experiments</p>
http://lod.bco-dmo.org/id/dataset-parameter/950258.rdf
Name: pH
Units: unitless
Description: <p>pH</p>
http://lod.bco-dmo.org/id/dataset-parameter/950259.rdf
Name: Total_Phosphorus
Units: microgram/Liter (ug/L)
Description: <p>Total Phosphorus</p>
http://lod.bco-dmo.org/id/dataset-parameter/950260.rdf
Name: Total_Phosphorus_95percent_CI
Units: microgram/Liter (ug/L)
Description: <p>Total Phosphorus 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950261.rdf
Name: Total_Dissolved_Phosphorus
Units: microgram/Liter (ug/L)
Description: <p>Total Dissolved Phosphorus</p>
http://lod.bco-dmo.org/id/dataset-parameter/950262.rdf
Name: Total_Dissolved_Phosphorus_95percent_CI
Units: microgram/Liter (ug/L)
Description: <p>Total Dissolved Phosphorus 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950263.rdf
Name: Nitrate
Units: miligram/Liter (mg/L)
Description: <p>Nitrate</p>
http://lod.bco-dmo.org/id/dataset-parameter/950264.rdf
Name: Nitrate_95percent_CI
Units: miligram/Liter (mg/L)
Description: <p>Nitrate 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950265.rdf
Name: Ammonium
Units: microgram/Liter (ug/L)
Description: <p>Ammonium</p>
http://lod.bco-dmo.org/id/dataset-parameter/950266.rdf
Name: Ammonium_95percent_CI
Units: microgram/Liter (ug/L)
Description: <p>Ammonium 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950267.rdf
Name: Soluble_Reactive_Phosphorus
Units: microgram/Liter (ug/L)
Description: <p>Soluble Reactive Phosphorus</p>
http://lod.bco-dmo.org/id/dataset-parameter/950268.rdf
Name: Soluble_Reactive_Phosphorus_95percent_CI
Units: microgram/Liter (ug/L)
Description: <p>Soluble Reactive Phosphorus 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950269.rdf
Name: Average_Incubation_Temperature
Units: degrees Celsius (°C)
Description: <p>Average Incubation Temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/950270.rdf
Name: Incubation_Temperature_Standard_Deviation
Units: degrees Celsius (°C)
Description: <p>Incubation Temperature Standard Deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/950271.rdf
Name: peak_A
Units: Raman units (RU)
Description: <p>Peak A is the fluorescence intensity of FDOM at excitation (ex) of 250 nm and emission (em) at 450 nm</p>
http://lod.bco-dmo.org/id/dataset-parameter/950272.rdf
Name: peak_A_95percent_CI
Units: Raman units (RU)
Description: <p>fDOM peak A 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950273.rdf
Name: peak_C
Units: Raman units (RU)
Description: <p>Peak C is the fluorescence intensity at ex and em of 350 and 450 nm, respectively</p>
http://lod.bco-dmo.org/id/dataset-parameter/950274.rdf
Name: peak_C_95percent_CI
Units: Raman units (RU)
Description: <p>fDOM peak C 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950275.rdf
Name: peak_T
Units: Raman units (RU)
Description: <p>Peak T is the fluorescence intensity at ex and em of 275 and 340 nm, respectively</p>
http://lod.bco-dmo.org/id/dataset-parameter/950276.rdf
Name: peak_T_95percent_CI
Units: Raman units (RU)
Description: <p>fDOM peak T 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950277.rdf
Name: peak_C_peak_A_ratio
Units: unitless
Description: <p>fDOM peak C/peak A ratio</p>
http://lod.bco-dmo.org/id/dataset-parameter/950278.rdf
Name: peak_C_peak_A_ratio_95percent_CI
Units: unitless
Description: <p>fDOM peak C/peak A ratio 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950279.rdf
Name: peak_T_peak_A_ratio
Units: unitless
Description: <p>fDOM peak T/peak A ratio</p>
http://lod.bco-dmo.org/id/dataset-parameter/950280.rdf
Name: peak_T_peak_A_ratio_95percent_CI
Units: unitless
Description: <p>fDOM peak T/peak A ratio 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950281.rdf
Name: Integrated_Fluorescence
Units: unitless
Description: <p>Integrated Fluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/950282.rdf
Name: Integrated_Fluorescence_95percent_CI
Units: unitless
Description: <p>Integrated Fluorescence 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950283.rdf
Name: Fluorescence_Index
Units: unitless
Description: <p>Fluorescence Index</p>
http://lod.bco-dmo.org/id/dataset-parameter/950284.rdf
Name: Fluorescence_Index_95percent_CI
Units: unitless
Description: <p>Fluorescence Index 95% Confidence Interval</p>
http://lod.bco-dmo.org/id/dataset-parameter/950285.rdf
Name: Slope_Ratio
Units: unitless
Description: <p>The Slope Ratio is the spectral slope ratio of the absorption curve of chromophoric dissolved organic matter (CDOM). The slope ratio is a unitless proxy for the average molecular weight of DOM</p>
http://lod.bco-dmo.org/id/dataset-parameter/950286.rdf
Name: Slope_Ratio_95percent_CI
Units: unitless
Description: <p>Slope Ratio 95% Confidence Interval</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/944935/data/download
download
onLine
dataset
<p>Water samples were collected in the western basin of Lake Erie during the summer and fall of 2017, 2018, and 2019.&nbsp; In 2017, water was collected approximately weekly from NOAA station WE2 in conjunction with the NOAA Great Lakes Environmental Research Lab (GLERL) harmful algal bloom monitoring program.&nbsp; During August and October 2017, lake water was also collected by Environment and Climate Change Canada’s monitoring program.&nbsp; In 2018 and 2019, lake water was collected at several stages of bloom development (pre-bloom, early bloom, late bloom, and post bloom).&nbsp; In 2018, lake water was collected at NOAA’s monitoring stations WE2 and WE12 and at the drinking water intake for the City of Toledo (TWI).&nbsp; During summer 2019, the goal was to sample lake waters containing high bloom biomass as predicted by the NOAA HAB forecast model and HAB tracker bulletins (Wynne et al. 2013).&nbsp; Sampling sites were chosen based on the presence of surface scums comprised of dense cyanobacterial colonies (i.e., “bloom chase” sites).</p>
<p>For all sites, a depth-integrated water sample was collected in acid-washed carboys.&nbsp; Water samples were collected from the NOAA stations using a peristaltic pump.&nbsp; The pump hose was moved down the water column from the surface to 1 meter above the bottom.&nbsp; For the TWI, bloom chase, and Environment Canada cruise sites, a depth-integrated sample was collected by pooling water collected at 1 m intervals from surface to 1 meter above the lake bottom using a Niskin (Environment Canada) or Van Dorn (TWI and bloom chase sites) bottle.&nbsp; Integrated water samples were stored in carboys in an outdoor aquaculture tank until the start of the bottle experiments the following morning.&nbsp; The water temperature in the aquaculture tank was controlled using copper piping attached to a NESLAB RTE refrigerated water bath (Thermo Scientific, Newington, NH) and maintained at the lake temperature measured at the time of sample collection.&nbsp; During the Environment Canada cruises, bottles and carboys were stored in a plexiglass tank continuously circulated with fresh lake water.</p>
<p>Subsamples for supporting water quality analyses were taken from each carboy.&nbsp; Upon arrival in the laboratory at the University of Michigan, a subsample of whole (unfiltered) water was taken for analysis of total phosphorus.&nbsp; During 2017, pH of the water from each site was obtained from NOAA monitoring buoys.&nbsp; For samples collected in 2018-2019, pH of the whole water was measured upon arrival in the laboratory.&nbsp;&nbsp; Subsamples of the whole water were filtered through a 0.22 μm polyethersulfone (PES) filter for subsequent analysis of total dissolved phosphorus (TDP), soluble reactive phosphorous, nitrate and ammonium, dissolved organic carbon (DOC), and chromophoric and fluorescent dissolved organic matter (CDOM and FDOM, respectively).&nbsp; DOC samples were preserved by addition of 6N trace metal grade hydrochloric acid to pH 3.&nbsp;&nbsp; TDP, SRP, DOC, CDOM and FDOM were stored in the dark at 4 °C until analysis.&nbsp; Nitrate and ammonium samples were stored at -20 °C until analysis at GLERL.</p>
<p>Subsamples for H<sub>2</sub>O<sub>2</sub> and DNA were collected by filtering 100-200 mL of water from each bottle through a 0.22 μm pore size PES filter and collecting the last 50 mL of filtrate into a centrifuge tube.&nbsp; The filtered water for H<sub>2</sub>O<sub>2</sub> analysis was stored in the dark at 4 °C until analysis within 4 hours of collection.&nbsp; H<sub>2</sub>O<sub>2</sub> concentrations were measured on an FeLume by flow injection analysis using standard additions as previously described (King et al. 2007, as applied to Lake Erie waters in Cory et al. 2017; Pandey et al. 2022).&nbsp; The filter was saved for DNA extraction by freezing in a cryovial containing 1 mL RNAlater at -80 °C.&nbsp;</p>
Specified by the Principal Investigator(s)
Processed the submitted file Environmental data BCO-DMO.xlsx with the BCO-DMO processing tool Laminar.
- Imported the submitted file into Laminar
- Renamed the parameters to conform with the BCO-DMO naming convention by replacing spaces with underscores, replacing % symbols with the word 'percent', and remove parenthesis.
- Converted dates from the format %d-%b-%y into the ISO 8601 format of %Y-%m-%d
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
NESLAB RTE refrigerated water bath
NESLAB RTE refrigerated water bath
PI Supplied Instrument Name: NESLAB RTE refrigerated water bath PI Supplied Instrument Description:NESLAB RTE refrigerated water bath made by Thermo Scientific, Newington, NH Instrument Name: circulating water bath Instrument Short Name: Instrument Description: A device designed to regulate the temperature of a vessel by bathing it in water held at the desired temperature. [Definition Source: NCI]
FeLume
FeLume
PI Supplied Instrument Name: FeLume PI Supplied Instrument Description:FeLume, Waterville Analytical, purchased 2011
The description from the company website of Waterville Analytical, http://watervilleanalytical.com/products.html
The FeLume(II) is a general purpose, FIA-based, analytical system for the analysis of Fe(II), Fe(III), Cu(II), Co(II), hydrogen peroxide, and superoxide. Instrument Name: Flow Injection Analyzer Instrument Short Name:FIA Instrument Description: An instrument that performs flow injection analysis. Flow injection analysis (FIA) is an approach to chemical analysis that is accomplished by injecting a plug of sample into a flowing carrier stream. FIA is an automated method in which a sample is injected into a continuous flow of a carrier solution that mixes with other continuously flowing solutions before reaching a detector. Precision is dramatically increased when FIA is used instead of manual injections and as a result very specific FIA systems have been developed for a wide array of analytical techniques. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB36/
Aqualog fluorometer
Aqualog fluorometer
PI Supplied Instrument Name: Aqualog fluorometer PI Supplied Instrument Description:Aqualog fluorometer made by Horiba Scientific Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
PI Supplied Instrument Name: Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
peristaltic pump
peristaltic pump
PI Supplied Instrument Name: peristaltic pump Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps