http://lod.bco-dmo.org/id/dataset/945544
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-12-05
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Response of Micromonas sp. to iron-limitation sampled aboard R/V Roger Revelle RR1813 in the Subarctic North Pacific near Station PAPA (50 N, 144.8 W) from August to September 2018
2025-04-04
publication
2025-04-04
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-04-07
publication
https://doi.org/10.26008/1912/bco-dmo.945544.1
Adrian Marchetti
University of North Carolina at Chapel Hill
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Meyer, M. G., Marchetti, A. (2025) Response of Micromonas sp. to iron-limitation sampled aboard R/V Roger Revelle RR1813 in the Subarctic North Pacific near Station PAPA (50 N, 144.8 W) from August to September 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-04-04 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.945544.1 [access date]
Response of Micromonas sp. to iron-limitation Dataset Description: Methods and Sampling: <p>Samples were taken at Ocean Station Papa, Subarctic North Pacific Ocean.&nbsp; Sampling depth during cruise RR1813 was 3 m. The culture was isolated from a sample taken at approximate location (lat:50, lon:-145). Micromonas cells were isolated using a 10 mL pipette after whole seawater sampling from a trace metal clean rosette aboard the R/V Revelle.&nbsp; Cultures were transported back to the University of North Carolina at Chapel Hill and stored in Aquil media under constant 120-150 µmol photons m-2 d-1 light in a 12ºC incubator.</p>
<p>Cultures were grown with either 1.37x10-6 M total Fe ("Fe+")&nbsp; or 3.10x10-9 M total Fe ("Fe-") media.&nbsp; Upon reaching exponential phase growth (based on trends in relative fluorescence units as measured by a Turner fluorometer),&nbsp; cultures were filtered for chlorophyll a concentration, particulate carbon/particulate nitrogen, and RNA. Additional samples for cell count were collected and fixed with a 10% addition of paraformaldehyde. Measurements of the maximum quantum yield of photochemistry in photosystem II (Fv/Fm) and the functional absorption cross section of photosystem II (sigma) were collected. Filters used in chlorophyll and carbon/nitrogen sampling were uncombusted and combusted Whatman 0.7µm glass fiber filters, respectively.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2219973 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2219973
completed
Adrian Marchetti
University of North Carolina at Chapel Hill
919-843-3473
123 South Rd.
Chapel Hill
NC
27514
USA
amarchet@email.unc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cruise
Latitude
Longitude
Treatment
Replicate
Volume
Cell_Count
Carbon
Nitrogen
CHL
Fv_Fm
Sigma
Growth_Rates
Guava easyCyte flow cytometer
Turner 10AU fluorometer
theme
None, User defined
cruise_id
latitude
longitude
treatment
replicate
volume
count
Elemental carbon (C)
Nitrogen
chlorophyll a
Fv to Fm ratio
No BCO-DMO term
growth
featureType
BCO-DMO Standard Parameters
Elemental Analyzer
Flow Cytometer
Turner Designs Fluorometer 10-AU
instrument
BCO-DMO Standard Instruments
RR1813
service
Deployment Activity
Subarctic North Pacific near Station PAPA (50 N, 144.8 W)
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Evaluating the contribution of small eukaryotes to nitrate-based new production in the North Pacific Subtropical Gyre
https://osprey.bco-dmo.org/project/914269
Collaborative Research: Evaluating the contribution of small eukaryotes to nitrate-based new production in the North Pacific Subtropical Gyre
<p>NSF abstract:<br />
The subtropical oceans in the middle latitudes are “ocean deserts,” where there are relatively little nutrients in the surface waters. Nitrogen is an important nutrient that is particularly scarce. Nitrogen in the ocean occurs as nitrate or ammonium. As a result of limited nutrients in the subtropical gyres, marine algae or phytoplankton there grow slowly. In deeper waters below the surface, there are more nutrients. However, it is not well understood how phytoplankton living in surface waters in summer can access the nutrients in the deeper waters. This award will investigate how phytoplankton access subsurface nitrogen at Station ALOHA in the North Pacific Subtropical Gyre (NPSG). Phytoplankton will be sorted by size and pigment composition. The researchers will make measurements of the stable nitrogen isotope ratios of sorted populations and determine where phytoplankton are getting the needed nutrients, either nitrate from the subsurface or ammonium from the surface waters. The researchers will use metatranscriptomic analysis to investigate the physiological pathways that different phytoplankton populations use to take up the needed nitrogen. The metatranscriptomic analysis involves analyzing the RNA sequences transcribed by plankton, comparing the sequence to documented gene sequences and mapped to documented physiological pathways. The researchers will assess which taxonomic groups are using nitrate versus recycled sources of nitrogen. They will test the hypothesis that eukaryotic phytoplankton primarily use nitrate whereas prokaryotic phytoplankton mostly use recycled sources of nitrogen. With these measurements, the researchers will help the society anticipate climate impacts on the productivity of subtropical gyres. This project will support two graduate students and a post-doctoral researcher in laboratory and ship-based research. Principal Investigator (PI) Granger in Connecticut will engage high school students and sponsor graduate students from underrepresented groups via AGU’s Bridge Partner Program. PI Marchetti in North Carolina will sponsor the internship of a high school student from a local minority county. PI White in Hawaii will engage with Hawaiian high school students though the Indigenous Partnership for Ocean Monitoring program to mentor and teach essential research skills.</p>
<p>The researchers of this project will investigate the drivers of productivity at station ALOHA in NPSG. They will exploit a high-sensitivity method for natural abundance N isotope analysis (the ‘persulphate-denitrifier’ method) to evaluate the extent to which taxonomically distinct components of the plankton sorted by flow cytometry rely on nitrate vs. reduced N sources in surface waters. We will also query physiological nitrogen pathways of the plankton with metatranscriptomic analysis to infer which clades are reliant on nitrate. We will quantify the fraction of primary production fueled by nitrate to characterize seasonal trends and explore potential mechanisms of nitrate supply to the euphotic zone and its mixed layer. The work proposed here will resolve the relative contribution of nitrate mixed from the ocean interior to surface production at station ALOHA, to better define seasonal dynamics of the biological pump in the NPSG. We will identify plankton groups that contribute dominantly to nitrate-based production and resolve eco-physiological strategies that confer fitness in a severely N-limited environment. Seasonal trends in the contributions of nitrate to total nitrogen production will be evaluated considering incident hydrography to infer potential mechanisms of nitrate supply.</p>
Small Euks
largerWorkCitation
project
eng; USA
oceans
Subarctic North Pacific near Station PAPA (50 N, 144.8 W)
-145
-145
50
50
2018-08-16
2018-09-07
Station ALOHA - North Pacific Subtropical Gyre
0
BCO-DMO catalogue of parameters from Response of Micromonas sp. to iron-limitation sampled aboard R/V Roger Revelle RR1813 in the Subarctic North Pacific near Station PAPA (50 N, 144.8 W) from August to September 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/945570.rdf
Name: Cruise
Units: unitless
Description: <p>Cruise ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/945571.rdf
Name: Latitude
Units: unitless
Description: <p>Approximate sampling latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/945572.rdf
Name: Longitude
Units: unitless
Description: <p>Approximat sampling longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/945573.rdf
Name: Treatment
Units: unitless
Description: <p>Treatment (NO3- Fe-, NO3- Fe+) - iron treatment, Fe- refers to Aquil media concentrations of 1.37 x 10-6 M Fe and Fe+ refers to Aquil media concentrations of 3.10 x 10-6 M Fe </p>
http://lod.bco-dmo.org/id/dataset-parameter/945574.rdf
Name: Replicate
Units: unitless
Description: <p>Replicate (A, B, C) - culture replicate </p>
http://lod.bco-dmo.org/id/dataset-parameter/945575.rdf
Name: Volume
Units: liter (L)
Description: <p>Measured volume</p>
http://lod.bco-dmo.org/id/dataset-parameter/945576.rdf
Name: Cell_Count
Units: cell per liter (cell L-1)
Description: <p>Cell Count</p>
http://lod.bco-dmo.org/id/dataset-parameter/945577.rdf
Name: Carbon
Units: grams (g)
Description: <p>Particulate carbon concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/945578.rdf
Name: Nitrogen
Units: grams (g)
Description: <p>Particulate nitrogen concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/957924.rdf
Name: CHL
Units: micrograms per liter (µg L-1)
Description: <p>Chlorophyll concentration extracted in acetone and analyzed fluorometrically according to Graff and Rynearson (2011)</p>
http://lod.bco-dmo.org/id/dataset-parameter/945579.rdf
Name: Fv_Fm
Units: unitless
Description: <p>Estimates of maximum photochemical yield of photosystem II</p>
http://lod.bco-dmo.org/id/dataset-parameter/945580.rdf
Name: Sigma
Units: unitless
Description: <p>Cross-sectional absorption area of photosystem II</p>
http://lod.bco-dmo.org/id/dataset-parameter/945581.rdf
Name: Growth_Rates
Units: per day (d-1)
Description: <p>Growth rates (d-1)</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
804
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/f1f6f191-2290-4a26-aa8e-a5368916cadb/content
download
https://doi.org/10.26008/1912/bco-dmo.945544.1
download
onLine
dataset
<p>Samples were taken at Ocean Station Papa, Subarctic North Pacific Ocean.&nbsp; Sampling depth during cruise RR1813 was 3 m. The culture was isolated from a sample taken at approximate location (lat:50, lon:-145). Micromonas cells were isolated using a 10 mL pipette after whole seawater sampling from a trace metal clean rosette aboard the R/V Revelle.&nbsp; Cultures were transported back to the University of North Carolina at Chapel Hill and stored in Aquil media under constant 120-150 µmol photons m-2 d-1 light in a 12ºC incubator.</p>
<p>Cultures were grown with either 1.37x10-6 M total Fe ("Fe+")&nbsp; or 3.10x10-9 M total Fe ("Fe-") media.&nbsp; Upon reaching exponential phase growth (based on trends in relative fluorescence units as measured by a Turner fluorometer),&nbsp; cultures were filtered for chlorophyll a concentration, particulate carbon/particulate nitrogen, and RNA. Additional samples for cell count were collected and fixed with a 10% addition of paraformaldehyde. Measurements of the maximum quantum yield of photochemistry in photosystem II (Fv/Fm) and the functional absorption cross section of photosystem II (sigma) were collected. Filters used in chlorophyll and carbon/nitrogen sampling were uncombusted and combusted Whatman 0.7µm glass fiber filters, respectively.</p>
Specified by the Principal Investigator(s)
<p>Chlorophyll was analyzed at the University of North Carolina on a Turner 10AU fluorometer following the protocol of Graff and Rynearson (2011). Particulate carbon/particulate nitrogen was analyzed on an elemental analyzer at the University of Maryland Center for Environmental Science Application Laboratory.&nbsp; Cell counts were measured at North Carolina State University on a Guava easyCyte flow cytometer equipped with a red laser.&nbsp;</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
* Renamed parameters to comply with database requirements
* Added cruise ID and apporximate sampling location to data
* Changed Bathycoccus sp. to Micromonas sp. by request of submitter (April 4, 2025)
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
Guava easyCyte flow cytometer
Guava easyCyte flow cytometer
PI Supplied Instrument Name: Guava easyCyte flow cytometer Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
Turner 10AU fluorometer
Turner 10AU fluorometer
PI Supplied Instrument Name: Turner 10AU fluorometer Instrument Name: Turner Designs Fluorometer 10-AU Instrument Short Name:Turner Fluorometer 10-AU Instrument Description: The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/
Cruise: RR1813
RR1813
R/V Roger Revelle
Community Standard Description
International Council for the Exploration of the Sea
R/V Roger Revelle
vessel
RR1813
Deborah K. Steinberg
Virginia Institute of Marine Science
https://datadocs.bco-dmo.org/docs/EXPORTS/data_docs/RR1813_Cruise_Report.pdf
Report describing RR1813
R/V Roger Revelle
Community Standard Description
International Council for the Exploration of the Sea
R/V Roger Revelle
vessel