http://lod.bco-dmo.org/id/dataset/945708
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-12-10
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Invertebrate stable isotopes from samples collected at methane seeps off Southern California with the HOV Alvin from July 16-28, 2023 during R/V Atlantis cruise AT50-12
2025-02-07
publication
2025-02-07
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-02-11
publication
https://doi.org/10.26008/1912/bco-dmo.945708.1
Lisa A. Levin
University of California-San Diego Scripps
principalInvestigator
María Emilia Emilia Bravo
Instituto Argentino de Oceanografía
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Pereira, O. S., Levin, L. A., Bravo, M. E. E. (2025) Invertebrate stable isotopes from samples collected at methane seeps off Southern California with the HOV Alvin from July 16-28, 2023 during R/V Atlantis cruise AT50-12. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-02-07 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.945708.1 [access date]
Invertebrate stable isotopes from Southern California seeps (July 2023) Dataset Description: Methods and Sampling: <p>Pushcore, rocks, biotubes, and slurp samples were collected with HOV Alvin using the manipulator. Rocks were placed into individual compartments in a biobox on the Alvin basket. Before preserving, samples were kept cold and animals were handpicked to sample tissue for stable isotope analyses.&nbsp;The tissue (aprox. 0.5-2.5 mg) was placed in pre weighted tin boats or combusted vials (500C overnight) and frozen at -70C. In&nbsp;addition to natural abundance analyses, some animals were placed into mylar bags and exposed to labeled C and N for incubation experiments. These were carried out for 3-8 days, and animals were then processed following the same method as for natural abundance samples. Surface and bottom seawater was collected using a CTD/Rosette system and filtered through microglass fiber filters. Filters were frozen for further processing in the laboratory.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2048720 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2048720
completed
Lisa A. Levin
University of California-San Diego Scripps
858-534-3579
9500 Gilman Drive Mail Code: 0218
La Jolla
CA
92093-0218
USA
llevin@ucsd.edu
pointOfContact
María Emilia Emilia Bravo
Instituto Argentino de Oceanografía
mebravo@gl.fcen.uba.ar
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
Alvin_Dive
Site
Latitude
Longitude
Depth
Sample_number
Habitat
Sample_type
Analysis_context
Phylum
Taxon_ID
Incubation
C13
N15
Remarks
CTD/Rosette system
oven
HOV Alvin
isotope ratio mass spectrometer
theme
None, User defined
date
dive_id
site
latitude
longitude
depth
sample identification
site description
sample description
phylum
taxon
experiment type
d13C
d15N
comments
featureType
BCO-DMO Standard Parameters
CTD Sea-Bird 911
Drying Oven
HOV Alvin
Isotope-ratio Mass Spectrometer
instrument
BCO-DMO Standard Instruments
AT50-12
service
Deployment Activity
Southern California Borderland
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Redefining the footprint of deep ocean methane seepage for benthic ecosystems
https://osprey.bco-dmo.org/project/840220
Collaborative Research: Redefining the footprint of deep ocean methane seepage for benthic ecosystems
<p><em>NSF Award Abstract:</em><br />
This research examines the role of deep-sea organisms in determining the fate and footprint of methane, a potent greenhouse gas, on Pacific continental margins. The investigators are evaluating the deep ocean methanosphere defined by the microbial communities that consume methane and the animals that directly feed on or form symbioses with methane-consuming microbes. They are also investigating animal communities that gain energy indirectly from methane, as well as those that take advantage of carbonate rocks, the physical manifestation of methane consumption in seafloor sediments. The study of methane seeps in the deep waters of both Alaska (4400-5500 meters) and Southern California (450-1040 meters) is enabling comparisons of the methanosphere under different food-limitation and oxygen regimes. By applying diverse chemical, isotopic, microscopy, and genetic-based analyses to seep microbes and fauna, this study is advancing understanding of the contribution of methane to deep-sea biodiversity and ecosystem function, information that can inform management and conservation actions in US waters. In addition to training for graduate and undergraduate students at their home institutions, the investigators are collaborating with the Alaska Native Science and Engineering Program (ANSEP). They are recruiting Alaskan undergraduates to participate in the research, contributing to ANSEP's online resources that promote interaction between scientists and middle and high school students, and participating in ANSEP's annual residential Career Exploration in Marine Science programs to engage middle school students in learning about deep-sea ecosystems and the variety of career pathways available in marine related fields.</p>
<p>Microbial production and consumption of methane is dynamic and widespread along continental margins, and some animals within deep-sea methane seeps rely on the oxidation and sequestration of methane for nutrition. At the same time, understanding of methane-dependent processes and symbioses in the deep-sea environment is still rudimentary. The goals of this study are to 1) examine the diversity of animals involved in methane-based symbioses and heterotrophic consumption of methane-oxidizing microbes and how these symbioses extend the periphery of seeps, contributing to non-seep, continental slope food webs; and 2) determine whether carbonates on the seep periphery sustain active methanotrophic microbial assemblages, providing a localized food source or chemical fuel for thiotrophic symbioses, via anaerobic oxidation of methane, or free-living, sulfide-oxidizing bacteria consumed by animals. The investigators are addressing these goals by surveying, sampling, and characterizing microbes, water, sediments, carbonates and animals at a deep seep site on the Aleutian Margin and a shallow site off Southern California. Shipboard experiments and laboratory analyses are using molecular, isotopic, geochemical, and radiotracer tools to understand transfer of methane-sourced carbon from aerobic methanotrophs under multiple oxygen levels, pressures, and photosynthetic food inputs. This approach offers a wide lens by which to examine the methane seep footprint, allow reinterpretation of past observations, and identify new scientific areas for future study. Improved characterization of the deep continental margin methanosphere informs climate science, biodiversity conservation, and resource management.</p>
Methanosphere
largerWorkCitation
project
eng; USA
biota
oceans
Southern California Borderland
-118.647282
-117.40573333333
32.6326
33.7997358
2023-07-16
2023-07-28
Gulf of Alaska and Southern California Bight
0
BCO-DMO catalogue of parameters from Invertebrate stable isotopes from samples collected at methane seeps off Southern California with the HOV Alvin from July 16-28, 2023 during R/V Atlantis cruise AT50-12
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/948930.rdf
Name: Date
Units: unitless
Description: <p>Date of collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/948931.rdf
Name: Alvin_Dive
Units: unitless
Description: <p>HOV Alvin dive number of collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/948932.rdf
Name: Site
Units: unitless
Description: <p>Methane seep site of collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/948933.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude in decimal degrees; positive values = North</p>
http://lod.bco-dmo.org/id/dataset-parameter/948934.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude in decimal degrees; positive values = East</p>
http://lod.bco-dmo.org/id/dataset-parameter/948935.rdf
Name: Depth
Units: meters (m)
Description: <p>Depth of collection in meters</p>
http://lod.bco-dmo.org/id/dataset-parameter/948936.rdf
Name: Sample_number
Units: unitless
Description: <p>Sample number</p>
http://lod.bco-dmo.org/id/dataset-parameter/948937.rdf
Name: Habitat
Units: unitless
Description: <p>Level of seepage activity at collection; Activity was categorized as Active, Inactive, Transition, no data, Edge of Seep, Less Active, Low Activity, Unknown, or Low Activity/None</p>
http://lod.bco-dmo.org/id/dataset-parameter/948938.rdf
Name: Sample_type
Units: unitless
Description: <p>Describes if it is a rock sample (carbonate or mudstone) or a pushcore sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/948939.rdf
Name: Analysis_context
Units: unitless
Description: <p>Indicates whether the sample was analyzed for its natural isotope abundance or as part of an incubation experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/948940.rdf
Name: Phylum
Units: unitless
Description: <p>Phylum of the specimen</p>
http://lod.bco-dmo.org/id/dataset-parameter/948941.rdf
Name: Taxon_ID
Units: unitless
Description: <p>Identification at the lowest taxonomic level possible of specimen</p>
http://lod.bco-dmo.org/id/dataset-parameter/948942.rdf
Name: Incubation
Units: unitless
Description: <p>For natural abundance samples it says "Natural abundance" and for incubation samples it says the treatment with labeled C and/or N of incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/948943.rdf
Name: C13
Units: per mil (0/00)
Description: <p>C isotope value in per mille</p>
http://lod.bco-dmo.org/id/dataset-parameter/948945.rdf
Name: N15
Units: per mil (0/00)
Description: <p>N isotope value in per mille</p>
http://lod.bco-dmo.org/id/dataset-parameter/948947.rdf
Name: Remarks
Units: unitless
Description: <p>General comments</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
156829
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/81e9db6e-dddd-4627-8b40-c609f7923464/content
download
https://doi.org/10.26008/1912/bco-dmo.945708.1
download
onLine
dataset
<p>Pushcore, rocks, biotubes, and slurp samples were collected with HOV Alvin using the manipulator. Rocks were placed into individual compartments in a biobox on the Alvin basket. Before preserving, samples were kept cold and animals were handpicked to sample tissue for stable isotope analyses.&nbsp;The tissue (aprox. 0.5-2.5 mg) was placed in pre weighted tin boats or combusted vials (500C overnight) and frozen at -70C. In&nbsp;addition to natural abundance analyses, some animals were placed into mylar bags and exposed to labeled C and N for incubation experiments. These were carried out for 3-8 days, and animals were then processed following the same method as for natural abundance samples. Surface and bottom seawater was collected using a CTD/Rosette system and filtered through microglass fiber filters. Filters were frozen for further processing in the laboratory.</p>
Specified by the Principal Investigator(s)
<p>In the laboratory, tissue samples were oven dried (60C) overnight, weighed and acidified with 12.5-25 ul 1N H3PO4 to remove inorganic C. Stable isotopes measurements (d13C and d15N) were carried out using an isotope ratio mass spectrometer at the Stable Isotope Facility at University of California, Davis. Microglass fiber filters were oven dried (60C) overnight, and the material on the filters was scrapped off and placed into pre-weighed tin boats. Samples were then&nbsp;processed following the same method as for tissue samples.</p>
Specified by the Principal Investigator(s)
- Imported "AT50-12_Isotope Data_BCO-DMO.xlsx" and "AT50-12_Sample information_BCO-DMO.xlsx" into BCO-DMO system
- Adjusted strings in sample names to correspond with sample names in sample info file, removing leading zeros and confirming with submitter that items should be mapped or corrected
-In the samples file "AT50-12_Sample information_BCO-DMO.xlsx", the following transformation were made:
*Replaced GFF #0 → GFF #
*Replaced phosphorte → phosphorite
-In the data file "AT50-12_Isotope Data_BCO-DMO.xlsx", the following transformations and mappings were made:
*Replaced Colonization wood SCB W11 → Colonization wood SCB-W11
*Replaced Colonization wood SCB W12 → Colonization wood SCB-W12
*Replaced Colonization bone CR6 → Colonization bone SCB-CR6
*Matched row 83 with Dive/Sample number AD5193/Rock 7
*Matched rows 551 and 552 with Dive/Sample number AD5197/Slurp 5
*Matched row 745 with Dive/Sample number AD5202/Rock 3,4
- Removed special characters to comply with UTC-8 requirements for system
- Joined "AT50-12_Isotope Data_BCO-DMO.xlsx" and "AT50-12_Sample information_BCO-DMO.xlsx" to incorporate sample metadata into primary dataset
- Converted date in both files to represent ISO YYYY-mm-dd
- Replaced 'Algae' with 'Ocrophyta' in Phylum parameter, confirmed by submitter
- Removed rows with 'LOST' values in C13, confirmed by submitter
- Replaced Plathyhelminthes with Platyhelminthes in Phylum parameter
- Exported dataset as "945708_v1_invertebrate_isotopes.csv" with supplemental file "at50-12_sample_information_bco-dmo-1.csv"
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
CTD/Rosette system
CTD/Rosette system
PI Supplied Instrument Name: CTD/Rosette system PI Supplied Instrument Description:Surface and bottom seawater was collected using a CTD/Rosette system and filtered through microglass fiber filters. Instrument Name: CTD Sea-Bird 911 Instrument Short Name:CTD SBE 911 Instrument Description: The Sea-Bird SBE 911 is a type of CTD instrument package. The SBE 911 includes the SBE 9 Underwater Unit and the SBE 11 Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). More information from Sea-Bird Electronics. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0035/
oven
oven
PI Supplied Instrument Name: oven PI Supplied Instrument Description:In the laboratory, tissue samples were oven dried (60C) overnight, weighed and acidified with 12.5-25 ul 1N H3PO4 to remove inorganic C. Instrument Name: Drying Oven Instrument Short Name: Instrument Description: a heated chamber for drying
HOV Alvin
HOV Alvin
PI Supplied Instrument Name: HOV Alvin PI Supplied Instrument Description:Pushcore, rocks, biotubes, and slurp samples were collected with HOV Alvin using the manipulator. Instrument Name: HOV Alvin Instrument Short Name: Instrument Description: Human Occupied Vehicle (HOV) Alvin is part of the National Deep Submergence Facility (NDSF). Alvin enables in-situ data collection and observation by two scientists to depths reaching 6,500 meters, during dives lasting up to ten hours.
Commissioned in 1964 as one of the world’s first deep-ocean submersibles, Alvin has remained state-of-the-art as a result of numerous overhauls and upgrades made over its lifetime. The most recent upgrades, begun in 2011 and completed in 2021, saw the installation of a new, larger personnel sphere with a more ergonomic interior; improved visibility and overlapping fields of view; longer bottoms times; new lighting and high-definition imaging systems; improved sensors, data acquisition and download speed. It also doubled the science basket payload, and improved the command-and-control system allowing greater speed, range and maneuverability.
With seven reversible thrusters, it can hover in the water, maneuver over rugged topography, or rest on the sea floor. It can collect data throughout the water column, produce a variety of maps and perform photographic surveys. Alvin also has two robotic arms that can manipulate instruments, obtain samples, and its basket can be reconfigured daily based on the needs of the upcoming dive.
Alvin's depth rating of 6,500m gives researchers in-person access to 99% of the ocean floor. Alvin is a proven and reliable platform capable of diving for up to 30 days in a row before requiring a single scheduled maintenance day. Recent collaborations with autonomous vehicles such as Sentry have proven extremely beneficial, allowing PIs to visit promising sites to collect samples and data in person within hours of their being discovered, and UNOLs driven technological advances have improved the ability for scientific outreach and collaboration via telepresence
Alvin is named for Allyn Vine, a WHOI engineer and geophysicist who helped pioneer deep submergence research and technology.
(from https://www.whoi.edu/what-we-do/explore/underwater-vehicles/hov-alvin/, accessed 2022-09-09)
isotope ratio mass spectrometer
isotope ratio mass spectrometer
PI Supplied Instrument Name: isotope ratio mass spectrometer PI Supplied Instrument Description:Stable isotopes measurements (d13C and d15N) were carried out using an isotope ratio mass spectrometer at the Stable Isotope Facility at University of California, Davis. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Cruise: AT50-12
AT50-12
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT50-12
Lisa A. Levin
University of California-San Diego Scripps
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel