http://lod.bco-dmo.org/id/dataset/945964
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-12-13
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Transparent Exopolymer Particles (TEP) from CTD samples collected during R/V Hugh R. Sharp cruise HRS2204 from Apr to May 2022
2025-02-27
publication
2025-02-27
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-02-27
publication
https://doi.org/10.26008/1912/bco-dmo.945964.1
Matthew Rau
Pennsylvania State University
principalInvestigator
Kieran Curran
University of New Hampshire
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Rau, M., Curran, K. (2025) Transparent Exopolymer Particles (TEP) from CTD samples collected during R/V Hugh R. Sharp cruise HRS2204 from Apr to May 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-02-27 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.945964.1 [access date]
Transparent Exopolymer Particles (TEP) CTD Bottle Measurements HRS2204 Dataset Description: Methods and Sampling: <p>This cruise visited eight stations on the Northeastern U.S. Continental Shelf. Latitudes and longitudes provided per sample in the data, but general station descriptions&nbsp;are below.</p>
<ul>
<li>Station 1 - Station 3: Georges Bank near the Gulf of Maine. Approximate location was 41.7 N, 68 W. Samples acquired from 0 m to 25 m&nbsp;depths.</li>
<li>Station 4: The Great South Channel near the Gulf of Maine. Approximate location was 41.6 N, 69 W. Samples acquired from 0 m to 150 m&nbsp;depths.</li>
<li>Station 5: Only one CTD profile was taken before this station was aborted due to weather. No data acquired. Station location was 40.8 N, 70.5 W.</li>
<li>Station 6: Off the coast of Martha's Vineyard. Approximate location was 41.3 N, 70.5 W. Samples acquired from 0 m to 10 m&nbsp;depths.</li>
<li>Station 7: At the mouth of the Sakonnet River near Newport, Rhode Island. Approximate location was 41.5 N, 71.2 W. Samples acquired from 0 m to 10 m&nbsp;depths.</li>
<li>Station 8: Hudson Canyon near New York. Approximate location was 39.5 N, 72.3 W. Samples acquired from 0 m to 200 m depths.</li>
</ul>
<p>Seawater samples between 500 and 2000 ml were vacuum filtered through a 0.45 micron polycarbonate membrane filter. Filter retentate was then stained with Alcian Blue solution. The Alcian Blue solution was made to a concentration of 400 mg/L by mixing dye powder with ultrapure water that was acidified to pH 2.5 with glacial acetic acid. After mixing, the stain was filtered through a 0.22 micron polycarbonate membrane to remove undissolved powder. Stained filters were frozen in Falcon tubes at -20 C for the remainder of the cruise and returned to the lab on dry ice for analysis. In the lab, TEP samples were extracted in 6ml 80% sulfuric acid solution for 2 hrs. The absorbance of this solution was then measured with a spectrophotometer at 787 nm. Concentration of TEP was converted from absorbance to microgram equivalent of Xanthan Gum through a calibration curve. Calibration was conducted following the procedure of Bittar, et al. (2018). A 5-point calibration was performed using dilutions of Xanthan Gum solution with concentrations of 9.37, 18.75, 37.50, 56.25, and 75 micrograms per ml in 1 mL polypropylene tubes. 0.5 ml of the Alcian Blue solution was added to these tubes, which were then mixed for 1 min through manual agitation. These stained aliquots were then poured onto filtration towers and filtered through 0.45 micron polycarbonate filters. The filter retentate was then extracted using the 80% sulfuric acid wash for spectrophotometric quantification. Filters were soaked in the solution for 2-20 hrs with regular agitation. The spectrophotometer (Agilent 8453) was first blanked with ultrapure water prior to measurements. 6 mL of extraction solution was read using 1.0 cm polystyrene disposable cuvettes (Fisher-Scientific).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1948283 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1948283
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1948685 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1948685
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2326735 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2326735
completed
Matthew Rau
Pennsylvania State University
matthew.rau@gwu.edu
pointOfContact
Kieran Curran
University of New Hampshire
kieran.curran@unh.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
ID
Station
CTD
Depth
ISO_DateTime_UTC
Latitude
Longitude
TEP_xg
CTD
Agilent 8453 UV-visible
theme
None, User defined
sample identification
station
cast
depth
ISO_DateTime_UTC
latitude
longitude
Transparent Exopolymer Particles
featureType
BCO-DMO Standard Parameters
CTD Sea-Bird 911
Spectrophotometer
instrument
BCO-DMO Standard Instruments
HRS2204
service
Deployment Activity
Northeastern U.S. Continental Shelf
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: The importance of particle disaggregation on biogeochemical flux predictions
https://osprey.bco-dmo.org/project/875305
Collaborative Research: The importance of particle disaggregation on biogeochemical flux predictions
<p>NSF abstract:</p>
<p>Particle settling is one of the major ways that material in surface waters reaches the deep ocean. Particulate matter in the open ocean consists primarily of organic material from plankton and other biological detritus, which can readily aggregate to form large flocs. A combination of physical, chemical, and biological processes transforms these flocs as they settle, redistributing material throughout the water column and potentially sequestering elements such as carbon in the deep ocean. The impact of these transformations is affected by the sinking speed of these flocs, with larger and denser particles settling faster than smaller, less-dense ones. One of the key questions facing oceanographers today is what controls particle settling speed (for example, particle size, shape, and density). There is considerable evidence that particles readily break apart as they settle, decreasing their average size and settling speed, but it is not yet understood what conditions cause these disaggregation events. This work will measure the breakup characteristics of organic settling particles both in the laboratory and at sea to quantify the importance of these breakup processes relative to particle transport. The work will be done at the Pennsylvania State University in collaboration with the University of Georgia to target the development of future marine particle disaggregation models for use by the oceanographic community.</p>
<p>This research will play an important role in determining the importance of disaggregation on the vertical transport of particulate matter in the ocean. The project will quantify the breakup of organic marine aggregates due to fluid forces caused by turbulence or swimming organisms. Phytoplankton will be cultured and formed into aggregates in the lab prior to disaggregation using calibrated turbulence. The size, shape, and structure of these aggregates before and after breakup will be quantified using high-speed visualization and holographic imaging. In addition to the laboratory measurements, a deployable instrument that can disrupt particles in-situ and measure their size and shape will be built and deployed in the North Atlantic during the spring bloom of phytoplankton. Detailed measurements of particle concentrations, breakup characteristics, organic content, and ambient turbulence as a function of depth in the water column will be collected. This work will represent the first study of marine aggregate breakup in-situ. Specifically, the project will clarify: (1) under what conditions disaggregation is important, (2) how strong different types of natural marine aggregates are and how their strength varies with size, composition, and morphology, and (3) how aggregate size, composition, and structure influences the distribution of its breakup mass. This project will advance the career of a doctoral student and engage numerous undergraduate researchers with the field of ocean science.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
<p> </p>
<p><u><strong>Additional Project Output (supplement to Data Collections section below):</strong></u></p>
<p><u>Model Code Description: </u></p>
<p>Adrian Burd's Research Lab. (2023). BurdLab/Dissaggregation: Disaggregation (Disaggregation). Zenodo. <a href="https://doi.org/10.5281/zenodo.8226166">https://doi.org/10.5281/zenodo.8226166</a></p>
<p><span style="font-size:12px"><span style="font-family:roboto,sans-serif">Associated Github Repository: </span></span><a href="https://github.com/BurdLab/Dissaggregation/tree/Disaggregation" style="box-sizing: border-box; background-color: transparent; color: rgb(66, 139, 202); text-decoration-line: none;">https://github.com/BurdLab/Dissaggregation/tree/Disaggregation</a> </p>
<p>This is the initial release of model code for particle aggregation and disaggregation in the ocean. The referenced Github Repository contains Matlab code to calculate the evolution of the particle size distribution in a single layer of the water column. The code numerically solves the aggregation-disaggregation mass balance equations using a so-called sectional approach developed by Gelbard and Seinfeld (J. Colloid and Interface Sci., 68:363-382, 1979). The model allows for particle aggregation, disaggregation, and sinking, and also changes in aggregate size from cell growth (see SetUpCoag.m), and will form the basis of a suite of particle aggrgation/disaggregation models. All documentation is provided within the code itself. Please see Associated Github Repository link above for detailed description and files. </p>
Disaggregation
largerWorkCitation
project
eng; USA
oceans
Northeastern U.S. Continental Shelf
-72.2765
-67.3996
39.4554
41.7749
2022-04-23
2022-05-01
Northeast United States Continental Shelf
0
BCO-DMO catalogue of parameters from Transparent Exopolymer Particles (TEP) from CTD samples collected during R/V Hugh R. Sharp cruise HRS2204 from Apr to May 2022
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/946016.rdf
Name: ID
Units: unitless
Description: <p>Identifier of the CTD cast sample, formatted as (station#_CTDcast#_depth)</p>
http://lod.bco-dmo.org/id/dataset-parameter/946017.rdf
Name: Station
Units: unitless
Description: <p>Station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/946018.rdf
Name: CTD
Units: unitless
Description: <p>CTD cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/946019.rdf
Name: Depth
Units: meters (m)
Description: <p>Depth in meters that the CTD bottle was triggered for sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/946020.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>ISO datetime the sample was acquired in UTC</p>
http://lod.bco-dmo.org/id/dataset-parameter/946023.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Ship's latitude when the sample was taken, N is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/946024.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Ship's longitude when the sample was taken, W is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/946025.rdf
Name: TEP_xg
Units: micrograms per liter (ug/L)
Description: <p>Mass concentration of TEP in Xanthan Gum equivalent, measured in micrograms per liter. Minimum detection limits were 5.6 micrograms per liter.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2793
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/6b41a69b-5d2a-4032-b608-94c6dfd010b7/content
download
https://doi.org/10.26008/1912/bco-dmo.945964.1
download
onLine
dataset
<p>This cruise visited eight stations on the Northeastern U.S. Continental Shelf. Latitudes and longitudes provided per sample in the data, but general station descriptions&nbsp;are below.</p>
<ul>
<li>Station 1 - Station 3: Georges Bank near the Gulf of Maine. Approximate location was 41.7 N, 68 W. Samples acquired from 0 m to 25 m&nbsp;depths.</li>
<li>Station 4: The Great South Channel near the Gulf of Maine. Approximate location was 41.6 N, 69 W. Samples acquired from 0 m to 150 m&nbsp;depths.</li>
<li>Station 5: Only one CTD profile was taken before this station was aborted due to weather. No data acquired. Station location was 40.8 N, 70.5 W.</li>
<li>Station 6: Off the coast of Martha's Vineyard. Approximate location was 41.3 N, 70.5 W. Samples acquired from 0 m to 10 m&nbsp;depths.</li>
<li>Station 7: At the mouth of the Sakonnet River near Newport, Rhode Island. Approximate location was 41.5 N, 71.2 W. Samples acquired from 0 m to 10 m&nbsp;depths.</li>
<li>Station 8: Hudson Canyon near New York. Approximate location was 39.5 N, 72.3 W. Samples acquired from 0 m to 200 m depths.</li>
</ul>
<p>Seawater samples between 500 and 2000 ml were vacuum filtered through a 0.45 micron polycarbonate membrane filter. Filter retentate was then stained with Alcian Blue solution. The Alcian Blue solution was made to a concentration of 400 mg/L by mixing dye powder with ultrapure water that was acidified to pH 2.5 with glacial acetic acid. After mixing, the stain was filtered through a 0.22 micron polycarbonate membrane to remove undissolved powder. Stained filters were frozen in Falcon tubes at -20 C for the remainder of the cruise and returned to the lab on dry ice for analysis. In the lab, TEP samples were extracted in 6ml 80% sulfuric acid solution for 2 hrs. The absorbance of this solution was then measured with a spectrophotometer at 787 nm. Concentration of TEP was converted from absorbance to microgram equivalent of Xanthan Gum through a calibration curve. Calibration was conducted following the procedure of Bittar, et al. (2018). A 5-point calibration was performed using dilutions of Xanthan Gum solution with concentrations of 9.37, 18.75, 37.50, 56.25, and 75 micrograms per ml in 1 mL polypropylene tubes. 0.5 ml of the Alcian Blue solution was added to these tubes, which were then mixed for 1 min through manual agitation. These stained aliquots were then poured onto filtration towers and filtered through 0.45 micron polycarbonate filters. The filter retentate was then extracted using the 80% sulfuric acid wash for spectrophotometric quantification. Filters were soaked in the solution for 2-20 hrs with regular agitation. The spectrophotometer (Agilent 8453) was first blanked with ultrapure water prior to measurements. 6 mL of extraction solution was read using 1.0 cm polystyrene disposable cuvettes (Fisher-Scientific).</p>
Specified by the Principal Investigator(s)
<p>Microsoft excel was used to create the linear calibration curve from the Xanthan Gum dilutions and to convert measured absorbance into mass of TEP in Xanthan Gum equivalents.</p>
Specified by the Principal Investigator(s)
- Imported "HRS22-04_TEP.xlsx" into the BCO-DMO system
- Corrected the import formatting which seems to have left off the seconds for time value on row 39; value is "11:45:10"
- Combined date and times to create datetime field
- Removed original date and time fields
- Round "TEP_xg" to 2 decimal based on submitter request
- Renamed fields to comply with BCO-DMO naming conventions
- Exported final file as "945964_v1_tep_ctd.csv"
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
CTD
CTD
PI Supplied Instrument Name: CTD PI Supplied Instrument Description:These data include measurements of transparent exopolymer particles (TEP) from CTD bottle water samples collected during a cruise on the Northeast Continental Shelf to study particle disaggregation. Instrument Name: CTD Sea-Bird 911 Instrument Short Name:CTD SBE 911 Instrument Description: The Sea-Bird SBE 911 is a type of CTD instrument package. The SBE 911 includes the SBE 9 Underwater Unit and the SBE 11 Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). More information from Sea-Bird Electronics. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0035/
Agilent 8453 UV-visible
Agilent 8453 UV-visible
PI Supplied Instrument Name: Agilent 8453 UV-visible PI Supplied Instrument Description:A spectrophotometer (Agilent 8453 UV-visible) was used to make the absorbance measurements, which were read using 1.0 cm polystyrene disposable cuvettes (Fisher-Scientific). Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Cruise: HRS2204
HRS2204
R/V Hugh R. Sharp
Community Standard Description
International Council for the Exploration of the Sea
R/V Hugh R. Sharp
vessel
HRS2204
Matthew Rau
Pennsylvania State University
R/V Hugh R. Sharp
Community Standard Description
International Council for the Exploration of the Sea
R/V Hugh R. Sharp
vessel