http://lod.bco-dmo.org/id/dataset/947067
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2024-12-27
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Seasonal fatty acid profiles of marine algae and invertebrates from Sitka Sound, Alaska in 2019
2024-12-27
publication
2024-12-27
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-01-07
publication
https://doi.org/10.26008/1912/bco-dmo.947067.1
Aaron W.E. Galloway
University of Oregon
principalInvestigator
Kristy J. Kroeker
University of California-Santa Cruz
principalInvestigator
Lauren E. Bell
University of California-Santa Cruz
principalInvestigator
Julie Schram
University of Oregon
principalInvestigator
Reyn Yoshioka
University of Oregon
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Galloway, A. W., Kroeker, K. J., Bell, L. E., Schram, J., Yoshioka, R. (2025) Seasonal fatty acid profiles of marine algae and invertebrates from Sitka Sound, Alaska in 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-12-27 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.947067.1 [access date]
Methods and Sampling: <p>We collected samples using SCUBA at three sites in Sitka Sound, all within 6 km of each other: Harris Island (N 57.03165, W 135.27754), Breast Island (N 57.03896, W 135.33309), and Samsing Pinnacle (N 56.98750, W 135.35718). Samples were collected during one week in two seasons: winter (January 2019) and summer (July 2019). Generally, we collected ~4 replicate samples for each species at each of the three sites in each season for fatty acids analysis. For seaweeds, this resulted in 4 replicates from one site in each season, and for invertebrates, this resulted in ~12 replicates (across 3 sites) for each season, with the exception of the isopod Pentidotea resecata in the summer, for which only one sample was collected due to a paucity of isopods residing in the seaweeds across our 3 sampling sites.<br />
<br />
We sampled six seaweeds, including three Laminarian kelps (Ochrophyta, urn:lsid:marinespecies.org:taxname:345465) and three red algae from the Gigartinales (urn:lsid:marinespecies.org:taxname:871)&nbsp;&nbsp;and Ceramiales (urn:lsid:marinespecies.org:taxname:860), that are present in both seasons in these sites: <em>Neoagarum fimbriatum, Macrocystis pyrifera, Hedophyllum nigripes, Cryptopleura ruprechtiana, Opuntiella californica, </em>and <em>Osmundea spectabilis&nbsp;</em>(See supplemental species_list.csv for identifiers).<br />
Note: "<em>Neoagarum fimbriatum</em>" appears as the&nbsp;unaccepted synonym "<em>Agarum fimbriatum</em>" used in this dataset.&nbsp;<br />
<br />
We sampled six macro-invertebrates (two each of gastropods, crustaceans, echinoderms) that are present and dominant herbivores in these sites: <em>Haliotis kamtschatkana, Tegula pulligo, Pentidotea resecata, Pugettia producta, Mesocentrotus franciscanus, and Strongylocentrotus droebachiensis&nbsp;</em>(See supplemental species_list.csv for identifiers).&nbsp;</p>
<p>M. pyrifera blades were collected from the distal ends of the thallus near the scimitar area from a boat at the surface, but other collections were made by SCUBA divers between 5-8 m depth. In general, all seaweeds were sampled in a consistent way within each species, targeting the same general tissues, avoiding proximal and distal areas of blades, avoiding sori, holdfasts, and stipes. All seaweeds were wiped clean with a paper towel and inspected to ensure they were not contaminated by epiphytes or endophytes. Invertebrates were sacrificed by freezing (-20°) for ~30 minutes before subsequent dissection. Invertebrate tissues were sampled in a consistent way for each taxon: Haliotis were sampled by snipping the foot muscle; Tegula were crushed with a hammer and the foot muscle was snipped; whole P. resecata individuals were sampled owing to their small size; for Pugettia, muscle tissue was removed from the legs; for Mesocentrotus and Strongylocentrotus, the test was cut open and gonadal tissue sampled. Sampling equipment was wiped clean with kimwipes and ethanol between samples. All samples were placed into 1.5 ml Eppendorf tubes, which were immediately frozen in a -20°C freezer. No solvents were introduced to samples in plastic storage vials.Within 3 days, all samples were moved to a -80°C freezer where they were stored until shipment to the -80°C freezer at the Oregon Institute of Marine Biology, where all fatty acid extractions were performed.&nbsp;</p>
<p>Tissue samples were kept at -80°C until extraction; all samples for both collection efforts were extracted within 9 months of collection. Samples were lyophilized to dryness for 48 hours. Once tissues were dried they were ground to fine, homogeneous powder with a stainless-steel mortar and pestle. Homogenized tissues were then immediately digested in chloroform for ~12 hrs sealed under nitrogen at -20°C. Total lipid extraction and subsequent derivatization of fatty acid methyl esters (FAME) was performed using a modification of the method described by (Taipale et al. 2016) and briefly summarized here. All sample extractions were performed in pre-combusted glassware.</p>
<p>Following initial tissue digestion in chloroform, 70 ul of unmethylated nonadecanoic acid (C19) was added as an internal standard. Total lipids were extracted using a 2:1:0.75 solution of chloroform: methanol: 0.9% NaCl solution, which was subsequently sonicated, vortexed, and centrifuged so that the more dense organic layer could be removed and evaporated to dryness under a steady stream of nitrogen gas. The organic phase was immediately resuspended in toluene and 1% sulfuric acid-methanol solution for 90 minutes at 90°C to transesterify FAME. Following transesterification, solutions were cooled to room temperature and KHCO3, to neutralize the acidic solution, and hexane was added. The combined solution was then vortexed and centrifuged to separate FAME in the hexane solution, which was concentrated to 1.5 ml in glass vials for gas chromatography.&nbsp;</p>
<p>FAME were analyzed with a gas chromatograph mass spectrometer (GC-MS, Shimadzu, Model QP2020), fitted with a DB-23 column (30 x 0.25mm x 0.15 µm, Agilent, Santa Clara, CA, USA), using helium as the carrier gas. To ensure sufficient separation between fatty acid peaks we utilized a heating program modified from Taipale et al. (2016) and described by Thomas et al. (2020). Individual fatty acids were identified using relative retention times of a FAME standard (GLC 566C, Nu-Chek Prep, Elysian, MN, USA) and specific ions. Fatty acids were quantified using integration of the major ion peaks (Taipale et al. 2016) with Shimadzu Lab Solutions software. Following identification and quantification, individual peak areas for all fatty acids identified were converted to proportions, representing the % contribution of all fatty acids identified in each sample.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1752600 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1752600
completed
Aaron W.E. Galloway
University of Oregon
agallow3@uoregon.edu
pointOfContact
Kristy J. Kroeker
University of California-Santa Cruz
831-566-8253
100 Shaffer Road
Santa Cruz
CA
95060
USA
kkroeker@ucsc.edu
pointOfContact
Lauren E. Bell
University of California-Santa Cruz
907-738-8007
312 Cascade St.
Sitka
AK
99835
USA
lbell@sitkascience.org
pointOfContact
Julie Schram
University of Oregon
jbschram@alaska.edu
pointOfContact
Reyn Yoshioka
University of Oregon
ryoshioka@bigelow.org
pointOfContact
asNeeded
Dataset Version: 1
Unknown
sampleID
season
site
Year_Month
lat
lon
spCode
type
genus
species
LSID
lengthDia_comment
lengthDia
height
wetWeightG
C12_0
C14_0
C14_1nX_1
C14_1nX_2
C14_1n5
i15_0
a15_0
C15_0
i16_0
C16_0
C16_1nX_1
C16_1nX_2
C16_1n7
C16_1nX_3
C16_1nX_4
i17_0
a17_0
C16_2nX_1
C16_2nX_2
C17_0
C16_3n4
C17_1
C16_4n1
i18_0
C18_0
C18_1nX_1
C18_1n9
C18_1n7c
C18_1nX_2
C18_2n6t
C18_2n6c
i19_0
C18_2
C18_3n6
C19_0
C18_3n3
C19_1
C18_4n3
C20_0
C20_1nX_1
C20_1n9
C20_1nX_2
C20_2nX_1
C20_1nX_3
C20_2nX_2
C20_2nX_3
C20_2n6
C20_3n6
C20_4n6
C20_3n3
C20_4n3
C20_5n3
C22_0
C22_1n9c
C22_2n6
C22_2nX
C22_4n6
C22_3n6
C22_4n3
C22_5n3
C22_6n3
C24_1n9
Gas chromatograph mass spectrometer (GC-MS, Shimadzu, Model QP2020)
theme
None, User defined
sample identification
season
site
Date_Month
latitude
longitude
taxon_code
animal group (category)
genus
species scientific name (binomial)
Life Science Identifier (LSID)
comments
length
height
wet_weight_biomass
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Gas Chromatograph Mass Spectrometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
CAREER: Energy fluxes and community stability in a dynamic, high-latitude kelp ecosystem
https://osprey.bco-dmo.org/project/756735
CAREER: Energy fluxes and community stability in a dynamic, high-latitude kelp ecosystem
<p><em>NSF Award Abstract:</em><br />
High latitude kelp forests support a wealth of ecologically and economically important species, buffer coastlines from high-energy storms, and play a critical role in the marine carbon cycle by sequestering and storing large amounts of carbon. Understanding how energy fluxes and consumer-resource interactions vary in these kelp communities is critical for defining robust management strategies that help maintain these valuable ecosystem services. In this integrated research and education program, the project team will investigate how consumer populations respond to variability in temperature, carbonate chemistry and resource quality to influence the food webs and ecosystem stability of kelp forests. A comprehensive suite of studies conducted at the northern range limit for giant kelp (Macrocystis pyrifera) in SE Alaska will examine how kelp communities respond to variable environmental conditions arising from seasonal variability and changing ocean temperature and acidification conditions. As part of this project, undergraduate and high school students will receive comprehensive training through (1) an immersive field-based class in Sitka Sound, Alaska, (2) intensive, mentored research internships, and (3) experiential training in science communication and public outreach that will include a variety of opportunities to disseminate research findings through podcasts, public lectures and radio broadcasts.</p>
<p>Consumer-resource interactions structure food webs and govern ecosystem stability, yet our understanding of how these important interactions may change under future climatic conditions is hampered by the complexity of direct and indirect effects of multiple stressors within and between trophic levels. For example, environmentally mediated changes in nutritional quality and chemical deterrence of primary producers have the potential to alter herbivory rates and energy fluxes between primary producers and consumers, with implications for ecosystem stability. Moreover, the effects of global change on primary producers are likely to depend on other limiting resources, such as light and nutrients, which vary seasonally in dynamic, temperate and high latitude ecosystems. In marine ecosystems at high latitude, climate models predict that ocean acidification will be most pronounced during the winter months, when primary production is limited by light. This project is built around the hypothesis that there could be a mismatch in the energetic demands of primary consumers caused by warming and ocean acidification and resource availability and quality during winter months, with cascading effects on trophic structure and ecosystem stability in the future. Through complementary lab and field experiments, the project team will determine 1) how temperature and carbonate chemistry combine to affect primary consumer bioenergetics across a diversity of species and 2) the indirect effects of ocean acidification and warming on primary consumers via environmentally mediated changes in the availability, nutritional quality and palatability of primary producers across seasons. Using the data from the laboratory and field experiments, the project team will 3) construct a model of the emergent effects of warming and ocean acidification on trophic structure and ecosystem stability in seasonally dynamic, high latitude environments.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
High latitude kelp dynamics
largerWorkCitation
project
eng; USA
oceans
-135.35718
-135.27754
56.9875
57.03165
2019-01-11
2019-07-14
SE Alaskan coastal waters
0
BCO-DMO catalogue of parameters from Seasonal fatty acid profiles of marine algae and invertebrates from Sitka Sound, Alaska in 2019
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/947414.rdf
Name: sampleID
Units: unitless
Description: <p>sample identifier, based on raw file created by the gas chromatographer-mass spectrometer</p>
http://lod.bco-dmo.org/id/dataset-parameter/947415.rdf
Name: season
Units: unitless
Description: <p>season of sampling, either winter or summer</p>
http://lod.bco-dmo.org/id/dataset-parameter/947416.rdf
Name: site
Units: unitless
Description: <p>site of specimen collection (BREAST = Breast Island, HARRIS = Harris Island, SAMSING = Samsing Pinnacle, SANDYCOVE = Sandy Cove)</p>
http://lod.bco-dmo.org/id/dataset-parameter/947417.rdf
Name: Year_Month
Units: unitless
Description: <p>Year and month of sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/947418.rdf
Name: lat
Units: decimal degrees
Description: <p>site latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/947419.rdf
Name: lon
Units: decimal degrees
Description: <p>site longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/947420.rdf
Name: spCode
Units: unitless
Description: <p>species shorthand code for specimen, primarily for recording and coding convenience, based on the genus name or a combination of genus name and species epithet. Note that SACCH is corrected to current name Hedophyllum nigripes in analysis and manuscript. See more information in the supplemental species_list.csv</p>
http://lod.bco-dmo.org/id/dataset-parameter/947421.rdf
Name: type
Units: unitless
Description: <p>guild of the specimen, Invert = intertebrates or herbivores, Algae = algae or producers</p>
http://lod.bco-dmo.org/id/dataset-parameter/947422.rdf
Name: genus
Units: unitless
Description: <p>Genus name of sampled specimen</p>
http://lod.bco-dmo.org/id/dataset-parameter/947423.rdf
Name: species
Units: unitless
Description: <p>Species epithet of sampled specimen</p>
http://lod.bco-dmo.org/id/dataset-parameter/947424.rdf
Name: LSID
Units: unitless
Description: <p>Life Science Identifier (LSID) for the taxonomic name used in the genus + species columns. See more information in the supplemental species_list.csv</p>
http://lod.bco-dmo.org/id/dataset-parameter/947425.rdf
Name: lengthDia_comment
Units: unitless
Description: <p>Comment explaining blanks in the lengthDia column (e.g. partial blade).</p>
http://lod.bco-dmo.org/id/dataset-parameter/947426.rdf
Name: lengthDia
Units: millimeters (mm)
Description: <p>length or diameter of specimen, relevant size measure dependent on taxon.</p>
http://lod.bco-dmo.org/id/dataset-parameter/947427.rdf
Name: height
Units: millimeters (mm)
Description: <p>height of specimen, relevant size measure dependent on taxon</p>
http://lod.bco-dmo.org/id/dataset-parameter/947431.rdf
Name: wetWeightG
Units: grams (g)
Description: <p>specimen wet weight</p>
http://lod.bco-dmo.org/id/dataset-parameter/947432.rdf
Name: C12_0
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 12:0, lauric acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/947433.rdf
Name: C14_0
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 14:0, myristic acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/947434.rdf
Name: C14_1nX_1
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 14:1, unknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947435.rdf
Name: C14_1nX_2
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 14:1, unknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947436.rdf
Name: C14_1n5
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 14:1 omega-5</p>
http://lod.bco-dmo.org/id/dataset-parameter/947437.rdf
Name: i15_0
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for iso 15:0, branched</p>
http://lod.bco-dmo.org/id/dataset-parameter/947438.rdf
Name: a15_0
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for anteiso 15:0, branched</p>
http://lod.bco-dmo.org/id/dataset-parameter/947439.rdf
Name: C15_0
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 15:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/947440.rdf
Name: i16_0
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for iso 16:0, branched</p>
http://lod.bco-dmo.org/id/dataset-parameter/947441.rdf
Name: C16_0
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 16:0, palmitic acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/947442.rdf
Name: C16_1nX_1
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 16:1, unknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947443.rdf
Name: C16_1nX_2
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 16:1, unknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947444.rdf
Name: C16_1n7
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 16:1 omega-7, palmitoleic acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/947445.rdf
Name: C16_1nX_3
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 16:1, unknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947446.rdf
Name: C16_1nX_4
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 16:1, unknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947447.rdf
Name: i17_0
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for iso 17:0, branched</p>
http://lod.bco-dmo.org/id/dataset-parameter/947448.rdf
Name: a17_0
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for anteiso 17:0, branched</p>
http://lod.bco-dmo.org/id/dataset-parameter/947449.rdf
Name: C16_2nX_1
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 16:2, uknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947450.rdf
Name: C16_2nX_2
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 16:2, uknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947451.rdf
Name: C17_0
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 17:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/947452.rdf
Name: C16_3n4
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 16:3 omega-4</p>
http://lod.bco-dmo.org/id/dataset-parameter/947453.rdf
Name: C17_1
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 17:1, uknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947454.rdf
Name: C16_4n1
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 16:4 omega-1</p>
http://lod.bco-dmo.org/id/dataset-parameter/947455.rdf
Name: i18_0
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for iso 18:0, branched</p>
http://lod.bco-dmo.org/id/dataset-parameter/947456.rdf
Name: C18_0
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 18:0, stearic acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/947457.rdf
Name: C18_1nX_1
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 17:1, uknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947458.rdf
Name: C18_1n9
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 18:1 omega-9, oleic acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/947459.rdf
Name: C18_1n7c
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 18:1 omega-7, cis, cis vaccenic acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/947460.rdf
Name: C18_1nX_2
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 18:1, uknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947461.rdf
Name: C18_2n6t
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 18:2 omega-6, trans</p>
http://lod.bco-dmo.org/id/dataset-parameter/947462.rdf
Name: C18_2n6c
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 18:2 omega-6, cis, linoleic acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/947463.rdf
Name: i19_0
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for iso 19:0, branched</p>
http://lod.bco-dmo.org/id/dataset-parameter/947464.rdf
Name: C18_2
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 18:2, uknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947465.rdf
Name: C18_3n6
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 18:3 omega-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/947466.rdf
Name: C19_0
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 19:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/947467.rdf
Name: C18_3n3
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 18:3 omega-3, alpha-linolenic acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/947468.rdf
Name: C19_1
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 19:1, uknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947469.rdf
Name: C18_4n3
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 18:4 omega-3, stearidonic acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/947470.rdf
Name: C20_0
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 20:0, arachidic acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/947471.rdf
Name: C20_1nX_1
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 20:1, uknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947472.rdf
Name: C20_1n9
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 20:1 omega-9</p>
http://lod.bco-dmo.org/id/dataset-parameter/947473.rdf
Name: C20_1nX_2
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 20:1, uknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947474.rdf
Name: C20_2nX_1
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 20:2, uknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947475.rdf
Name: C20_1nX_3
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 20:1, uknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947476.rdf
Name: C20_2nX_2
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 20:2, uknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947477.rdf
Name: C20_2nX_3
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 20:2, uknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947478.rdf
Name: C20_2n6
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 20:2 omega-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/947479.rdf
Name: C20_3n6
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 20:3 omega-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/947480.rdf
Name: C20_4n6
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 20:4 omega-6, arachidonic acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/947481.rdf
Name: C20_3n3
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 20:3 omega-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/947482.rdf
Name: C20_4n3
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 20:4 omega-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/947483.rdf
Name: C20_5n3
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 20:5 omega-3, eicosapentaenoic acid or EPA</p>
http://lod.bco-dmo.org/id/dataset-parameter/947484.rdf
Name: C22_0
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 22:0, behenic acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/947485.rdf
Name: C22_1n9c
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 22:1 omega-9</p>
http://lod.bco-dmo.org/id/dataset-parameter/947486.rdf
Name: C22_2n6
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 22:2 omega-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/947487.rdf
Name: C22_2nX
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 22:2, uknown omega double bond location</p>
http://lod.bco-dmo.org/id/dataset-parameter/947488.rdf
Name: C22_4n6
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 22:4 omega-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/947489.rdf
Name: C22_3n6
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 22:3 omega-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/947490.rdf
Name: C22_4n3
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 22:4 omega-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/947491.rdf
Name: C22_5n3
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 22:5 omega-3, docosapentaenoic acid or DPA</p>
http://lod.bco-dmo.org/id/dataset-parameter/947492.rdf
Name: C22_6n3
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 22:6 omega-3, docosahexaenoic acid or DHA</p>
http://lod.bco-dmo.org/id/dataset-parameter/947493.rdf
Name: C24_1n9
Units: micrograms (mg)
Description: <p>fatty acid quantity of sample determined via GC-MS for 24:1 omega-9</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
85800
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/ffc0bfd0-e547-49f6-a4ba-06eeae1a4825/content
download
https://doi.org/10.26008/1912/bco-dmo.947067.1
download
onLine
dataset
<p>We collected samples using SCUBA at three sites in Sitka Sound, all within 6 km of each other: Harris Island (N 57.03165, W 135.27754), Breast Island (N 57.03896, W 135.33309), and Samsing Pinnacle (N 56.98750, W 135.35718). Samples were collected during one week in two seasons: winter (January 2019) and summer (July 2019). Generally, we collected ~4 replicate samples for each species at each of the three sites in each season for fatty acids analysis. For seaweeds, this resulted in 4 replicates from one site in each season, and for invertebrates, this resulted in ~12 replicates (across 3 sites) for each season, with the exception of the isopod Pentidotea resecata in the summer, for which only one sample was collected due to a paucity of isopods residing in the seaweeds across our 3 sampling sites.<br />
<br />
We sampled six seaweeds, including three Laminarian kelps (Ochrophyta, urn:lsid:marinespecies.org:taxname:345465) and three red algae from the Gigartinales (urn:lsid:marinespecies.org:taxname:871)&nbsp;&nbsp;and Ceramiales (urn:lsid:marinespecies.org:taxname:860), that are present in both seasons in these sites: <em>Neoagarum fimbriatum, Macrocystis pyrifera, Hedophyllum nigripes, Cryptopleura ruprechtiana, Opuntiella californica, </em>and <em>Osmundea spectabilis&nbsp;</em>(See supplemental species_list.csv for identifiers).<br />
Note: "<em>Neoagarum fimbriatum</em>" appears as the&nbsp;unaccepted synonym "<em>Agarum fimbriatum</em>" used in this dataset.&nbsp;<br />
<br />
We sampled six macro-invertebrates (two each of gastropods, crustaceans, echinoderms) that are present and dominant herbivores in these sites: <em>Haliotis kamtschatkana, Tegula pulligo, Pentidotea resecata, Pugettia producta, Mesocentrotus franciscanus, and Strongylocentrotus droebachiensis&nbsp;</em>(See supplemental species_list.csv for identifiers).&nbsp;</p>
<p>M. pyrifera blades were collected from the distal ends of the thallus near the scimitar area from a boat at the surface, but other collections were made by SCUBA divers between 5-8 m depth. In general, all seaweeds were sampled in a consistent way within each species, targeting the same general tissues, avoiding proximal and distal areas of blades, avoiding sori, holdfasts, and stipes. All seaweeds were wiped clean with a paper towel and inspected to ensure they were not contaminated by epiphytes or endophytes. Invertebrates were sacrificed by freezing (-20°) for ~30 minutes before subsequent dissection. Invertebrate tissues were sampled in a consistent way for each taxon: Haliotis were sampled by snipping the foot muscle; Tegula were crushed with a hammer and the foot muscle was snipped; whole P. resecata individuals were sampled owing to their small size; for Pugettia, muscle tissue was removed from the legs; for Mesocentrotus and Strongylocentrotus, the test was cut open and gonadal tissue sampled. Sampling equipment was wiped clean with kimwipes and ethanol between samples. All samples were placed into 1.5 ml Eppendorf tubes, which were immediately frozen in a -20°C freezer. No solvents were introduced to samples in plastic storage vials.Within 3 days, all samples were moved to a -80°C freezer where they were stored until shipment to the -80°C freezer at the Oregon Institute of Marine Biology, where all fatty acid extractions were performed.&nbsp;</p>
<p>Tissue samples were kept at -80°C until extraction; all samples for both collection efforts were extracted within 9 months of collection. Samples were lyophilized to dryness for 48 hours. Once tissues were dried they were ground to fine, homogeneous powder with a stainless-steel mortar and pestle. Homogenized tissues were then immediately digested in chloroform for ~12 hrs sealed under nitrogen at -20°C. Total lipid extraction and subsequent derivatization of fatty acid methyl esters (FAME) was performed using a modification of the method described by (Taipale et al. 2016) and briefly summarized here. All sample extractions were performed in pre-combusted glassware.</p>
<p>Following initial tissue digestion in chloroform, 70 ul of unmethylated nonadecanoic acid (C19) was added as an internal standard. Total lipids were extracted using a 2:1:0.75 solution of chloroform: methanol: 0.9% NaCl solution, which was subsequently sonicated, vortexed, and centrifuged so that the more dense organic layer could be removed and evaporated to dryness under a steady stream of nitrogen gas. The organic phase was immediately resuspended in toluene and 1% sulfuric acid-methanol solution for 90 minutes at 90°C to transesterify FAME. Following transesterification, solutions were cooled to room temperature and KHCO3, to neutralize the acidic solution, and hexane was added. The combined solution was then vortexed and centrifuged to separate FAME in the hexane solution, which was concentrated to 1.5 ml in glass vials for gas chromatography.&nbsp;</p>
<p>FAME were analyzed with a gas chromatograph mass spectrometer (GC-MS, Shimadzu, Model QP2020), fitted with a DB-23 column (30 x 0.25mm x 0.15 µm, Agilent, Santa Clara, CA, USA), using helium as the carrier gas. To ensure sufficient separation between fatty acid peaks we utilized a heating program modified from Taipale et al. (2016) and described by Thomas et al. (2020). Individual fatty acids were identified using relative retention times of a FAME standard (GLC 566C, Nu-Chek Prep, Elysian, MN, USA) and specific ions. Fatty acids were quantified using integration of the major ion peaks (Taipale et al. 2016) with Shimadzu Lab Solutions software. Following identification and quantification, individual peak areas for all fatty acids identified were converted to proportions, representing the % contribution of all fatty acids identified in each sample.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Shimadzu Lab Solutions software.<br />
<br />
Note [2024-12-30] The spCode (species codes) column was&nbsp;not changed in the data and subsequent analysis. However, the genus and species associated with the codes were updated as follows:<br />
<br />
* spCode&nbsp;"AGFIM" was originally associated with the scientific name&nbsp;"Agarum fimbriatum" (LSID&nbsp;urn:lsid:marinespecies.org:taxname:372699). The genus and species were updated in the data and metadata&nbsp;to use the currently accepted name for the organism&nbsp;"Neoagarum fimbriatum" (LSID urn:lsid:marinespecies.org:taxname:1313053).<br />
* The scientific name associated with the code "SACCH" was updated from "Saccharina sp." to "Hedophyllum nigripes." Organisms originally identified as "Saccharina sp." were later reclassified as "Hedophyllum nigripes" (LSID&nbsp;urn:lsid:marinespecies.org:taxname:1356699).<br />
* spCode "OSMUNDIA" "Osmundia spectabalis" and "Osmundia spectabilis" corrected to:<br />
OSMUNDIA "Osmundea spectabilis"&nbsp; (LSID urn:lsid:marinespecies.org:taxname:372624)&nbsp;</p>
Specified by the Principal Investigator(s)
* Data table within submitted file "pHAKFA_FA_conc_pub.csv" was imported into the BCO-DMO data system for this dataset. Values "NA" imported as missing data values. The missing data identifiers were described as NA = not applicable.
** In the BCO-DMO data system missing data identifiers are displayed according to the format of data you access. For example, in csv files it will be blank (null) values. In Matlab .mat files it will be NaN values. When viewing data online at BCO-DMO, the missing value will be shown as blank (null) values.
* Year_Month column added.
* lengthDia_comment column added to hold string notes that were in the lengthDia column ("partial blade").
* Taxnomomic name checking and dataset update: World Register of Marine Species taxa match tool used to find misspellings and match names to identifier "Life Science Identifier" (LSID) on 2024-12-27.
* Changes were made to the genus and species columns in the dataset after a discussion with data submitter. Name spelling matches the currently accepted names for the organisms as of the time of this dataset's publication. A note in the "Data Processing Description" metadata section was added to clarify why the codes may not all look like a representation of the genus and species names in the dataset. And to clarify a re-classification of one of the codes.
* Species list with the species codes, scientific names, LSID, taxon status and match quality were added as a supplemental file.
* Supplemental site list table extracted from provided metadata and added as a supplemental file.
* lengthDia and height columns contained variable units described in additional column lengthHeightUnits as either "mm" or "cm". Columns with consistent units in mm added for lengthDia_mm and height_mm instead.
* lat and lon for SANDYCOVE added to site_list.csv and primary data table from coordinates supplied by the data contributors.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Gas chromatograph mass spectrometer (GC-MS, Shimadzu, Model QP2020)
Gas chromatograph mass spectrometer (GC-MS, Shimadzu, Model QP2020)
PI Supplied Instrument Name: Gas chromatograph mass spectrometer (GC-MS, Shimadzu, Model QP2020) PI Supplied Instrument Description:Gas chromatograph mass spectrometer (GC-MS, Shimadzu, Model QP2020), fitted with a DB-23 column (30 x 0.25mm x 0.15 µm, Agilent, Santa Clara, CA, USA), using helium as the carrier gas. Instrument Name: Gas Chromatograph Mass Spectrometer Instrument Short Name: Instrument Description: Instruments separating gases, volatile substances or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay by a mass spectrometer.