http://lod.bco-dmo.org/id/dataset/948025
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-01-07
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Coral Isotope data from a heating experiment using samples collected from Nikko Bay and Rebotel Reef in Palau in the spring of 2018
2025-01-10
publication
2025-01-10
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-01-10
publication
https://doi.org/10.26008/1912/bco-dmo.948025.1
Mark E. Warner
University of Delaware
principalInvestigator
Dustin Kemp
University of Alabama at Birmingham
principalInvestigator
Todd Christopher LaJeunesse
Pennsylvania State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Warner, M. E., Kemp, D., LaJeunesse, T. C. (2025) Coral Isotope data from a heating experiment using samples collected from Nikko Bay and Rebotel Reef in Palau in the spring of 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-01-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.948025.1 [access date]
Palau Coral Reef Experiment 2018: Isotopes Dataset Description: <p>This work was conducted in the island nation of Palau. Coral colonies were sampled from an inshore location (Ngermid Bay, also known as Nikko Bay) and an offshore location on the western barrier reef surrounding Palau (Rebotel Reef). Sampled colonies were returned to land and treated in a thermal experiment at the Palau International Coral Reef Center in land-based aquariums.</p> Methods and Sampling: <p>Eight colonies of the coral <em>Psammocora digitata </em>and<em> Pocillopora verrucosa </em>were sampled from the offshore western barrier reef, Rebotel reef (7.248833° N, 134.235817° E) at 5–10 m depth, and from Nikko Bay (also known by Ngermid Bay, 7.3245° N, 134.4939° E) at 5 m depth. Samples were transported to the Palau International Coral Research Center (PICRC), and each colony sample was cut into nine replicate ramets that were placed in flow-through sea water tables and allowed to heal for 48 hours before mounting on labeled PVC tiles with marine epoxy (Splash zone compound A-788).</p>
<p>Temperature experiments were conducted in indoor aquarium systems. Each system used a semi-enclosed design that consisted of a series of 44 L plastic bins connected to a central 220 L sump that was supplied by a continuous slow-feed supply of fresh seawater. The control system (4 bins) was maintained at an average temperature of 28.27 ± 0.33°C by an in-line chiller and titanium heater. The heated system (6 bins) was ramped from 28°C to 31°C (1°C day<sup>-1</sup>) and held at 31.86 ±0.14°C by an in-line titanium heater. All bins were lighted to an irradiance of 600 μmol photons m<sup>-2</sup>s<sup>-1 </sup>by LED lights set to daily ramping. At the end of each experiment (day 13), control and treatment ramets were placed in glass beakers containing 400 mL of freshly filtered seawater (0.45 µm) that was enriched with 0.633 mM of NaH<sup>13</sup>CO<sub>3</sub> (99 atom % <sup>13</sup>C, Cambridge Isotope Lab Inc., Andover, MA, USA), and 1.5 µM of Na<sup>15</sup>NO<sub>3</sub> (98 atom % <sup>15</sup>N, Cambridge Isotope Lab Inc., Andover, MA, USA). Beakers were fitted with false bottoms and continually stirred with magnetic stir bars. All beakers were held for 5 hours at the experimental temperatures under the continuous maximum light level (600 μmol photons m<sup>-2</sup>s<sup>-1</sup>) used in the experiment. Fragments were then removed, rinsed in filtered seawater, and immediately frozen at -60° C until further processing.</p>
<p>Coral tissue was removed with an airbrush and filtered seawater (0.45µm), followed by the addition of 0.02% (w/v) sodium dodecyl sulfate (SDS) and homogenization for 10 s with a Tissue-Tearor (Biospec Products, Inc). Algae and coral tissue were separated by 2–3 centrifugation washes (550 g for 5 min) with 10 s homogenization steps between each wash. Algal cells were pelleted via centrifugation and frozen at -20°C. Accumulated supernatants (animal portion) were microscopically verified to not contain symbiotic algae or skeletal material and were filtered onto pre-combusted (450°C for 5 h) glass 0.7 μm glass fiber filters (Whatman GF/F) until clogged and then frozen at −20°C. Due to the relatively high concentration of <sup>13</sup>C assimilation by the symbiotic algae during the labeling incubations, coral skeletons were placed in 100% bleach for 24h to remove remnant organic material, rinsed in fresh water for 24h, and dried under low heat. Approximately 20 mg of the outermost CaCO<sub>3</sub> was sampled from both the corallite and coenosarc regions of the coral skeleton using a Dremel tool with a diamond bit. Skeletal samples were stored at -20°C. Elemental isotope analyses were performed on a Carlo Erba CHN Elemental Analyzer (Model NA1500) coupled to Thermo Finnigan Delta V Isotope Ratio Mass Spectrometer via a Thermo Finnigan Conflo III Interface at the University of Georgia, Center for Applied Isotope Studies.</p>
<p>Instruments:</p>
<ul>
<li>Seawater temperature was controlled by an in-line chiller and titanium heater DeltaStar DS-3, and Cygnet Mini (Aqualogic Inc.), and light was supplied to each experimental bin by a custom LED array (XP-G3 Cool White LEDs, Cree) controlled with a digital Storm Controller(Coralux).</li>
<li>Water was continuously mixed in each bin by a small submersible pump (Sicce Micra, 90 GPH).</li>
<li>Coral tissue was removed with an airbrush (Paasche VL-3AS) at 100 psi and homogenized with a hand-held homogenizer (Tissue tearor, Biospec Products, Inc).</li>
<li>Coral homogenates were centrifuged in a clinical centrifuge (IEC)</li>
<li>Isotope analyses were performed on a Carlo Erba CHN Elemental Analyzer (Model NA1500) coupled to Thermo Finnigan Delta V Isotope Ratio Mass Spectrometer via a Thermo Finnigan Conflo III Interface at the University of Georgia, Center for Applied Isotope Studies.</li>
</ul>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1719684 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1719684
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1635695 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1635695
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1636022 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1636022
completed
Mark E. Warner
University of Delaware
302-645-4365
School of Marine Science and Policy 700 Pilottown Rd.
Lewes
DE
19958
USA
mwarner@udel.edu
pointOfContact
Dustin Kemp
University of Alabama at Birmingham
205-934-1426
1720 Second Ave South
Birmingham
AL
35294
US
dwk@uab.edu
pointOfContact
Todd Christopher LaJeunesse
Pennsylvania State University
814-863-2038
208 Mueller Laboratory Department of Biology
University Park
PA
16802
USA
tcl3@psu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Location_Name
Longitude
Latitude
Species
Sym
Treatment
Date
Day
Colony
a_13catom
h_13catom
s_13catom
a_15natom
h_15natom
Carlo Erba CHN Elemental Analyzer (Model NA1500)
Thermo Finnigan Delta V Isotope Ratio Mass Spectrometer
theme
None, User defined
region
longitude
latitude
species scientific name (binomial)
sample description
date
day of month
Elemental carbon (C)
Nitrogen
featureType
BCO-DMO Standard Parameters
Carlo-Erba NA-1500 Elemental Analyzer
Isotope-ratio Mass Spectrometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Stability, flexibility, and functionality of thermally tolerant coral symbioses
https://osprey.bco-dmo.org/project/708866
Collaborative Research: Stability, flexibility, and functionality of thermally tolerant coral symbioses
<p>NSF abstract:</p>
<p>All reef-building corals require large numbers of internal symbiotic microalgae (called Symbiodinium) for their survival and growth. These mutualisms have shown considerable sensitivity to changes in the environment in recent decades, especially due to global increases in ocean temperatures. When exposed to severe thermal stress, corals loose their symbionts and often die. However, recent experiments show that some symbionts may be more stress-tolerant. Corals with these heat-resistant symbionts continue to receive high amounts of algal derived nutrients and grow under elevated temperatures. If the global trend in seawater warming continues to increase, these heat-resistant symbioses may become more ecologically prevalent on reef systems around the world and could play a critical role in maintaining healthy and productive coral communities. This project will examine the ecological and physiological attributes of stress-tolerant symbioses from the Indo Pacific where coral communities are the largest, most diverse, and productive in the world. The researchers will conduct a series of experiments to (1) evaluate host and symbiont attributes that contribute to thermal tolerance and (2) characterize the relative flexibility and functionality of various corals and symbionts exposed to typical ambient and stressful temperatures. Broader impacts of the project include the training of several Ph.D. students, undergraduates, and high school students in the disciplines of physiology and ecology. The researchers will partner with Global Ocean Exploration, Inc. to communicate this research to the general public through short documentary videos, editorials, and podcasts. An interactive K-5 program, "Invertebrates on the Road," will introduce elementary students in Pennsylvania to marine invertebrate diversity. Research results will also be disseminated to the public at the University of Delaware via educational seminars, as well as through hands-on research displays and demonstrations presented at the annual open house "Coast Day" festival in each year of the project.</p>
<p>This project will examine several attributes important to the functional ecology of coral-dinoflagellate symbioses. Specifically, the research team seeks to understand the interplay between coral and symbiont physiologies under different environmental conditions and determine the relative influence of biotic factors crucial to the performance of stress tolerant symbioses. Results from recent experiments on Indo-west Pacific corals found that Clade D (S. trenchii) symbionts are stress-tolerant. These symbionts are able to maintain function and provide nutrients to their hosts under high temperatures that typically elicit the breakdown of symbioses involving many other species of symbiont. A number of questions arise about how enhanced thermal tolerance symbioses may be aided by a combination of factors; for example: Are symbionts physiologically hardier in corals that are routinely feeding? Do host genotypes that are adapted to high temperatures affect the physiology of their symbionts in ways that make the partnership more stress-tolerant? A series of experiments over three years will examine the functionality of different coral-symbiont pairings exposed to ambient and high temperatures. Reciprocal transplants between inshore (stress-tolerant) and offshore (stress-susceptible) reef sites will be used to produce specific host-symbiont parings. Controlled experiments will test the relative importance of coral trophic status (nutrient content) while holding symbiont type constant and how changes in both coral trophic status and symbiont species identity of the resident affect thermal tolerance. Tank experiments on shore will track rates of photosynthesis as well as carbon translocation and assimilation from symbiont to host tissues and skeletons. Long-term growth rates via skeletal density, linear extension, and biomass gain will also be measured. This project will help elucidate how biochemical, physiological and ecological differences among host-symbiont pairings may respond to rising ocean temperatures and enhance the future viability of coral reefs.</p>
Thermally tolerant coral
largerWorkCitation
project
eng; USA
oceans
134.235817
134.4939
7.248833
7.3245
2018-05-21
2018-06-03
Coral Reefs of Palau, Micronesia
0
BCO-DMO catalogue of parameters from Coral Isotope data from a heating experiment using samples collected from Nikko Bay and Rebotel Reef in Palau in the spring of 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/948266.rdf
Name: Location_Name
Units: unitless
Description: <p>Name of sampled reef</p>
http://lod.bco-dmo.org/id/dataset-parameter/948267.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Sampling longitude, west is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/948268.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Sampling latitude, south is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/948269.rdf
Name: Species
Units: unitless
Description: <p>Coral species tested. Psammacora digitata or Pocillopora verrucosa</p>
http://lod.bco-dmo.org/id/dataset-parameter/948270.rdf
Name: Sym
Units: unitless
Description: <p>Symbiotic dinoflagellate ID based on ITS2 nomenclature or formal genus and species (when known)</p>
http://lod.bco-dmo.org/id/dataset-parameter/948271.rdf
Name: Treatment
Units: unitless
Description: <p>Control (=28 degrees Celsius) or Heated (=32 degrees Celsius)</p>
http://lod.bco-dmo.org/id/dataset-parameter/948272.rdf
Name: Date
Units: unitless
Description: <p>Month Day Year as (MDDYYYY)</p>
http://lod.bco-dmo.org/id/dataset-parameter/948273.rdf
Name: Day
Units: unitless
Description: <p>Day of measurement from start of experiment (time zero)</p>
http://lod.bco-dmo.org/id/dataset-parameter/948274.rdf
Name: Colony
Units: unitless
Description: <p>Colony number (1–8 for each species)</p>
http://lod.bco-dmo.org/id/dataset-parameter/948275.rdf
Name: a_13catom
Units: percent (%)
Description: <p>Atom percent enrichment in 13C in the algae</p>
http://lod.bco-dmo.org/id/dataset-parameter/948276.rdf
Name: h_13catom
Units: percent (%)
Description: <p>Atom percent enrichment in 13C in the coral tissue</p>
http://lod.bco-dmo.org/id/dataset-parameter/948277.rdf
Name: s_13catom
Units: percent (%)
Description: <p>Atom percent enrichment in 13C in the coral skeleton</p>
http://lod.bco-dmo.org/id/dataset-parameter/948278.rdf
Name: a_15natom
Units: percent (%)
Description: <p>Atom percent enrichment in 15N in the algae</p>
http://lod.bco-dmo.org/id/dataset-parameter/948279.rdf
Name: h_15natom
Units: percent (%)
Description: <p>Atom percent enrichment in 15N in the coral tissue</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
7889
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/96b74ef6-24cf-44af-bf15-148b169a3a85/content
download
https://doi.org/10.26008/1912/bco-dmo.948025.1
download
onLine
dataset
<p>Eight colonies of the coral <em>Psammocora digitata </em>and<em> Pocillopora verrucosa </em>were sampled from the offshore western barrier reef, Rebotel reef (7.248833° N, 134.235817° E) at 5–10 m depth, and from Nikko Bay (also known by Ngermid Bay, 7.3245° N, 134.4939° E) at 5 m depth. Samples were transported to the Palau International Coral Research Center (PICRC), and each colony sample was cut into nine replicate ramets that were placed in flow-through sea water tables and allowed to heal for 48 hours before mounting on labeled PVC tiles with marine epoxy (Splash zone compound A-788).</p>
<p>Temperature experiments were conducted in indoor aquarium systems. Each system used a semi-enclosed design that consisted of a series of 44 L plastic bins connected to a central 220 L sump that was supplied by a continuous slow-feed supply of fresh seawater. The control system (4 bins) was maintained at an average temperature of 28.27 ± 0.33°C by an in-line chiller and titanium heater. The heated system (6 bins) was ramped from 28°C to 31°C (1°C day<sup>-1</sup>) and held at 31.86 ±0.14°C by an in-line titanium heater. All bins were lighted to an irradiance of 600 μmol photons m<sup>-2</sup>s<sup>-1 </sup>by LED lights set to daily ramping. At the end of each experiment (day 13), control and treatment ramets were placed in glass beakers containing 400 mL of freshly filtered seawater (0.45 µm) that was enriched with 0.633 mM of NaH<sup>13</sup>CO<sub>3</sub> (99 atom % <sup>13</sup>C, Cambridge Isotope Lab Inc., Andover, MA, USA), and 1.5 µM of Na<sup>15</sup>NO<sub>3</sub> (98 atom % <sup>15</sup>N, Cambridge Isotope Lab Inc., Andover, MA, USA). Beakers were fitted with false bottoms and continually stirred with magnetic stir bars. All beakers were held for 5 hours at the experimental temperatures under the continuous maximum light level (600 μmol photons m<sup>-2</sup>s<sup>-1</sup>) used in the experiment. Fragments were then removed, rinsed in filtered seawater, and immediately frozen at -60° C until further processing.</p>
<p>Coral tissue was removed with an airbrush and filtered seawater (0.45µm), followed by the addition of 0.02% (w/v) sodium dodecyl sulfate (SDS) and homogenization for 10 s with a Tissue-Tearor (Biospec Products, Inc). Algae and coral tissue were separated by 2–3 centrifugation washes (550 g for 5 min) with 10 s homogenization steps between each wash. Algal cells were pelleted via centrifugation and frozen at -20°C. Accumulated supernatants (animal portion) were microscopically verified to not contain symbiotic algae or skeletal material and were filtered onto pre-combusted (450°C for 5 h) glass 0.7 μm glass fiber filters (Whatman GF/F) until clogged and then frozen at −20°C. Due to the relatively high concentration of <sup>13</sup>C assimilation by the symbiotic algae during the labeling incubations, coral skeletons were placed in 100% bleach for 24h to remove remnant organic material, rinsed in fresh water for 24h, and dried under low heat. Approximately 20 mg of the outermost CaCO<sub>3</sub> was sampled from both the corallite and coenosarc regions of the coral skeleton using a Dremel tool with a diamond bit. Skeletal samples were stored at -20°C. Elemental isotope analyses were performed on a Carlo Erba CHN Elemental Analyzer (Model NA1500) coupled to Thermo Finnigan Delta V Isotope Ratio Mass Spectrometer via a Thermo Finnigan Conflo III Interface at the University of Georgia, Center for Applied Isotope Studies.</p>
<p>Instruments:</p>
<ul>
<li>Seawater temperature was controlled by an in-line chiller and titanium heater DeltaStar DS-3, and Cygnet Mini (Aqualogic Inc.), and light was supplied to each experimental bin by a custom LED array (XP-G3 Cool White LEDs, Cree) controlled with a digital Storm Controller(Coralux).</li>
<li>Water was continuously mixed in each bin by a small submersible pump (Sicce Micra, 90 GPH).</li>
<li>Coral tissue was removed with an airbrush (Paasche VL-3AS) at 100 psi and homogenized with a hand-held homogenizer (Tissue tearor, Biospec Products, Inc).</li>
<li>Coral homogenates were centrifuged in a clinical centrifuge (IEC)</li>
<li>Isotope analyses were performed on a Carlo Erba CHN Elemental Analyzer (Model NA1500) coupled to Thermo Finnigan Delta V Isotope Ratio Mass Spectrometer via a Thermo Finnigan Conflo III Interface at the University of Georgia, Center for Applied Isotope Studies.</li>
</ul>
Specified by the Principal Investigator(s)
<p>Enriched isotopic data are reported as atom% of the heavy isotope (AP<sup>15</sup>N &amp; AP<sup>13</sup>C).</p>
Specified by the Principal Investigator(s)
* Converted date to ISO format
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Carlo Erba CHN Elemental Analyzer (Model NA1500)
Carlo Erba CHN Elemental Analyzer (Model NA1500)
PI Supplied Instrument Name: Carlo Erba CHN Elemental Analyzer (Model NA1500) PI Supplied Instrument Description:Elemental isotope analyses were performed on a Carlo Erba CHN Elemental Analyzer (Model NA1500) Instrument Name: Carlo-Erba NA-1500 Elemental Analyzer Instrument Short Name:Carlo-Erba NA-1500 Instrument Description: A laboratory instrument that simultaneously determines total nitrogen and total carbon from a wide range of organic and inorganic sediment samples. The sample is completely and instantaneously oxidised by flash combustion, which converts all organic and inorganic substances into combustion products. The resulting combustion gases pass through a reduction furnace and are swept into the chromatographic column by the carrier gas which is helium. The gases are separated in the column and detected by the thermal conductivity detector which gives an output signal proportional to the concentration of the individual components of the mixture. The instrument was originally manufactured by Carlo-Erba, which has since been replaced by Thermo Scientific (part of Thermo Fisher Scientific). This model is no longer in production. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0470/
Thermo Finnigan Delta V Isotope Ratio Mass Spectrometer
Thermo Finnigan Delta V Isotope Ratio Mass Spectrometer
PI Supplied Instrument Name: Thermo Finnigan Delta V Isotope Ratio Mass Spectrometer Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/