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            <gco:CharacterString>Cite this dataset as: Cram, J. A., Fuchsman, C., Huanca Valenzuela, P., Tully, B., Frank, J., Sylvan, J. B. (2025) Dissolved nitrate plus nitrite, nitrite and phosphate from water column of the East Pacific Rise in April 2019 aboard the R/V Atlantis cruise AT42-09. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-02-04 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.948718.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Dataset Description: &amp;lt;p&amp;gt;These data were supported by NSF award OCE-1756339 and Horn Point Laboratory startup funds.&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
Results publication in review:&amp;lt;br /&amp;gt;
Fuchsman, C.A. and Cram, J.A. (n.d.) Size fractionated suspended organic carbon and nitrogen from the offshore Eastern Tropical North Pacific Oxygen Deficient Zone suggest contributions of picocyanobacteria and vertically migrating metazoans to organic matter. Global Biogeochemical Cycles. In review.&amp;lt;br /&amp;gt;
*preprint available at ESS Open Archive (Fuchsman &amp;amp;amp; Cram 2024, doi:10.22541/essoar.173046855.50289201/v1)&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
Huanca, P., C.A. Fuchsman, B.J. Tully, J.B. Sylvan, J.A. Cram (n.d.) Quantitative microbial taxonomy across particle size, depth, and oxygen concentration. Frontiers in Microbiology. In review.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;div&amp;gt;&amp;lt;span style=&amp;quot;font-size:13px&amp;quot;&amp;gt;[See &amp;quot;Related Publications&amp;quot; section for full references and links to methods and standards cited here.]&amp;lt;/span&amp;gt;&amp;lt;br /&amp;gt;
&amp;amp;nbsp;
&amp;lt;div&amp;gt;&amp;lt;span style=&amp;quot;font-size:13px&amp;quot;&amp;gt;Water was collected from the CTD rosette and prefiltered through a 0.2 μm Sterivex filter into a triplle rinsed 50 ml falcon tube. Samples were stored at -80 °C, shipped to University of Southern California on dry ice, stored for one year, and then shipped on to UMCES. At UMCES prefiltered (0.2 µm) dissolved nitrate, nitrite, and phosphate from the water samples were analyzed at the Nutrient Analytical Services Laboratory at the Chesapeake Biological Laboratory (CBL). The ELAP Certification Number for all samples is 12066 Methods for each nutrient follow:&amp;lt;/span&amp;gt;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;amp;nbsp;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;lt;span style=&amp;quot;font-size:13px&amp;quot;&amp;gt;Nitrite plus nitrate:&amp;amp;nbsp;&amp;amp;nbsp;&amp;lt;/span&amp;gt;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;amp;nbsp;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;lt;span style=&amp;quot;font-size:13px&amp;quot;&amp;gt;&amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp;Measured by using the Method EPA 353.2 - CADMIUM (in the case of samples AT4209-007-Surf, and AT2309-007-Chla-Top) and method ASTDM D-7781-14 for all other samples.&amp;lt;/span&amp;gt;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;amp;nbsp;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;lt;span style=&amp;quot;font-size:13px&amp;quot;&amp;gt;&amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp;Method detection limit was 0.0004 for method EPA 353.2 - CADMIUM and 0.0057 for method ASTM D-7781-14. Reporting limit was 0.0056 for method EPA 353.2 - CADMIUM and 0.028 for method ASTM D-7781-14.&amp;lt;/span&amp;gt;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;amp;nbsp;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;lt;span style=&amp;quot;font-size:13px&amp;quot;&amp;gt;Nitrate with the method EPA 353.2&amp;lt;/span&amp;gt;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;lt;span style=&amp;quot;font-size:13px&amp;quot;&amp;gt;&amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp;Method&amp;amp;nbsp; detection limit&amp;amp;nbsp; was 0.0009 and reporting limit was 0.0032&amp;lt;/span&amp;gt;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;amp;nbsp;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;lt;span style=&amp;quot;font-size:13px&amp;quot;&amp;gt;Phosphate by using method EPA 365.1&amp;lt;/span&amp;gt;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;lt;span style=&amp;quot;font-size:13px&amp;quot;&amp;gt;&amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp; Method detection limit was 0.0034 and reporting limit was 0.0102&amp;lt;/span&amp;gt;&amp;lt;/div&amp;gt;
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Hydrothermal vents, which deposit seafloor massive sulfides (SMS), occur along the 89,000 km of mid-ocean ridges, submarine volcanoes, and backarc basins that occur at tectonic plate boundaries in the ocean. Active hydrothermal vent sulfide chimneys are hotspots of biodiversity and productivity in the deep ocean, as well as potential resources for metals. While significant effort has focused on understanding the diversity of biological communities and geochemistry associated with actively venting SMS, relatively little is known about the biological communities associated with SMS once venting ceases. Furthermore, little is known about the microbiological and geochemical changes that occur during the transition period from active to inactive, during which an important succession occurs in the microbial community and geochemistry of fluids within the chimney. This interdisciplinary project will create and sample this transition period by collecting multiple active SMS samples from individual vents at 9 degrees N East Pacific Rise and allowing them to transition to inactive on the seafloor, mimicking the end of venting while allowing for the exact time when venting ceased to be known, something not possible when sampling naturally formed inactive SMS. Microbial community diversity and metabolism will be analyzed in parallel with bulk and fine-scale geological measurements for active, transitioning, and inactive sulfides. This seafloor experimental and analytical approach will provide knowledge of how microbial communities, rates of biogeochemical transformations, and geological conditions change as SMS transition from hot and actively venting to cold and inactive. Students in grades 6-8 will be entrained into the project through research cruise &quot;ship-to-shore&quot; interactions and communications, post-cruise workshops for educators working with students typically underrepresented in STEM fields, and a collaboration with the Science, Engineering, Art and Design Gallery (SEAD), a community and economic development project in Bryan, TX.&lt;/p&gt;
&lt;p&gt;Hydrothermal vents are quantitatively important to the biology and chemistry of the deep ocean, but the vast majority of current knowledge focuses on actively venting deposits. However, after venting ceases, sulfides can persist on the seafloor for tens of thousands of years, making them long-lived, globally-abundant microbial substrates. In recent years, studies of inactive SMS found drastically different microbial communities than those on active deposits, indicating a succession of the microbial community, and thus a potentially different impact on deep ocean biodiversity and biogeochemistry than actively venting deposits. However, ages of the inactive structures are often not known, so it is impossible to estimate how quickly these changes occur, and how quickly co-occurring changes in sulfide mineralogy and microbiological communities occur. This project will provide the first insight into what happens at the microbial and mineralogical level as SMS initially transition from active to inactive. Active SMS will be sampled and analyzed for microbial community composition, functional capacity, gene expression and metabolic rates. Co-located subsamples will be analyzed for porosity and bulk and fine-scale mineralogy. Subsamples of those active SMS samples will be left on the seafloor to incubate and be collected weeks and a year or more later, with the same analyses conducted upon collection. This will allow for determination of microbiological and mineralogical changes that occur during that initial transition and for comparison with older inactive SMS from the same vent fields. Together, the data collected will be integrated to generate a conceptual model of succession of biology, mineralogy, porosity and pore distribution as vent deposits transition from active to inactive. This project will fill a knowledge gap about hydrothermal ecosystems and has the potential to transform the current understanding of diversity and rates of change in these important seafloor biomes.&lt;/p&gt;</gco:CharacterString>
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	Name: NO23
	Units: milligrams per liter (mg N/L)
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	Name: PO4
	Units: milligrams per liter (mg P/L)
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&amp;amp;nbsp;
&amp;lt;div&amp;gt;&amp;lt;span style=&amp;quot;font-size:13px&amp;quot;&amp;gt;Water was collected from the CTD rosette and prefiltered through a 0.2 μm Sterivex filter into a triplle rinsed 50 ml falcon tube. Samples were stored at -80 °C, shipped to University of Southern California on dry ice, stored for one year, and then shipped on to UMCES. At UMCES prefiltered (0.2 µm) dissolved nitrate, nitrite, and phosphate from the water samples were analyzed at the Nutrient Analytical Services Laboratory at the Chesapeake Biological Laboratory (CBL). The ELAP Certification Number for all samples is 12066 Methods for each nutrient follow:&amp;lt;/span&amp;gt;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;amp;nbsp;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;lt;span style=&amp;quot;font-size:13px&amp;quot;&amp;gt;Nitrite plus nitrate:&amp;amp;nbsp;&amp;amp;nbsp;&amp;lt;/span&amp;gt;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;amp;nbsp;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;lt;span style=&amp;quot;font-size:13px&amp;quot;&amp;gt;&amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp;Measured by using the Method EPA 353.2 - CADMIUM (in the case of samples AT4209-007-Surf, and AT2309-007-Chla-Top) and method ASTDM D-7781-14 for all other samples.&amp;lt;/span&amp;gt;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;amp;nbsp;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;lt;span style=&amp;quot;font-size:13px&amp;quot;&amp;gt;&amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp;Method detection limit was 0.0004 for method EPA 353.2 - CADMIUM and 0.0057 for method ASTM D-7781-14. Reporting limit was 0.0056 for method EPA 353.2 - CADMIUM and 0.028 for method ASTM D-7781-14.&amp;lt;/span&amp;gt;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;amp;nbsp;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;lt;span style=&amp;quot;font-size:13px&amp;quot;&amp;gt;Nitrate with the method EPA 353.2&amp;lt;/span&amp;gt;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;lt;span style=&amp;quot;font-size:13px&amp;quot;&amp;gt;&amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp;Method&amp;amp;nbsp; detection limit&amp;amp;nbsp; was 0.0009 and reporting limit was 0.0032&amp;lt;/span&amp;gt;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;amp;nbsp;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;lt;span style=&amp;quot;font-size:13px&amp;quot;&amp;gt;Phosphate by using method EPA 365.1&amp;lt;/span&amp;gt;&amp;lt;/div&amp;gt;

&amp;lt;div&amp;gt;&amp;lt;span style=&amp;quot;font-size:13px&amp;quot;&amp;gt;&amp;amp;nbsp; &amp;amp;nbsp; &amp;amp;nbsp; Method detection limit was 0.0034 and reporting limit was 0.0102&amp;lt;/span&amp;gt;&amp;lt;/div&amp;gt;
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                <gco:CharacterString>&amp;lt;p&amp;gt;Data were processed by Chesapeake Bay Laboratory Analytical Services using their standard pipeline.&amp;amp;nbsp; Final data files for upload to BCO DMO were curated by hand by Jacob Cram.&amp;lt;/p&amp;gt;</gco:CharacterString>
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                  <gco:CharacterString>Data Version 1:
* data within the submitted file &amp;quot;EPR_Nutrients_for_BCODMO_W.csv&amp;quot;, was imported into the BCO-DMO data system for this dataset. Several revisions of this file were uploaded, but the version that corresponds to public version 1 of this dataset &amp;quot;EPR_Nutrients_for_BCODMO_W.csv&amp;quot; was uploaded 2025-02-17.

* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores.  Can not start with a number]

* Dates converted to ISO 8601 format

* ISO DateTime with timezone (UTC) column added in ISO 8601 format.

* latitude and longitude columns added from values provided in submission metadata.</gco:CharacterString>
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