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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/948735.rdf" xlink:actuate="onRequest">Sinking Organic Particle fluxes and stable C isotopes (collected with sediment traps) from the Eastern Tropical North Pacific on the R/V Sikuliaq cruise SKQ201617S in January 2017</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Fuchsman, C., Duffy, M. E., Devol, A., Keil, R., Neibauer, J. A. (2025) Sinking Organic Particle fluxes and stable C isotopes (collected with sediment traps) from the Eastern Tropical North Pacific on the R/V Sikuliaq cruise SKQ201617S in January 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-01-17 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.948735.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Methods and Sampling: &amp;lt;p&amp;gt;Free floating, unpoisoned surface tethered sediment traps were used to quantify fluxes of sinking particles at St P2&amp;amp;nbsp;&amp;amp;nbsp;(16.5ºN&amp;amp;nbsp;107ºW)&amp;amp;nbsp;in January 2017, deployed from the R/V Sikuliaq on cruise SKQ201617S. Trap depths ranged between 69 m and 965 m, and trap deployments ranged between 21 and 91 hours with deeper traps deployed for longer. Traps were deployed in arrays of two traps per line. Two types of traps were deployed: 1) in shallow waters (&amp;amp;lt;150 m), traps with a solid plastic cone top (0.46 m&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt; opening area) were used, 2) in deep waters (&amp;amp;gt;150 m), net traps (1.24 m&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt; opening area) modeled from (Peterson et al., 2005) were used. For both types of trap, the cod end had bottoms that were open during deployment and during an 8 hour equilibration period at the target depth performed to remove oxygen contamination. Cod ends were closed with a gate valve, using a pre-programmed electronic dissolving link (burn wire) system controlled by an onboard Arduino microcontroller to start collection at the correct depth, and a second gate valve that closed the top of the cod end before retrieval. The aspect ratio, or height/trap mouth diameter, of the net traps was 2.5 while the aspect ratio of the cone traps was 0.6. These two types of traps have been shown to collect material with similar efficiency when normalized by opening area&amp;amp;nbsp;(Cram et al., 2022).&amp;amp;nbsp;No salt solution was used with these traps and no poisons were used in any chamber.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Some trap deployments functioned as simple sediment traps, and some deployments were combined trap and in situ incubators. The combined trap incubators consisted of upper and lower chambers. The material used to calculate fluxes reported here was collected from the upper chamber and was not incubated.&amp;amp;nbsp; Sinking material was collected in the lower incubation chamber, then isolated by closed a gate valve and injected with &amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N-NO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;-&amp;lt;/sup&amp;gt; . While the incubation occurred (in situ), sinking material was collected in an upper chamber that never encountered the spiked material. However, we do not report d&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N natural stable isotopes for the sediment trap material here due to proximity to the incubation.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;After every deployment, sediment trap material was filtered onto pre-combusted GF-75 filters (0.3 µm nominal pore size). To conform to community standards, zooplankton carcasses were not included in the measurements of carbon and nitrogen flux.&amp;amp;nbsp;Filter samples (particles only) were wafted with HCl overnight to remove carbonate, dried at 40°C, packed into silver foil cups, and sent to the University of Washington Isolab facility in the Department of Earth and Space Sciences (Seattle, WA) for C and N analysis utilizing an Costech elemental analyzer &amp;amp;nbsp;attached to an isotope ratio mass spectrometer (ThermoFinnegan MAT 253).&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/665100.rdf" xlink:title="DEB-1542240" xlink:actuate="onRequest">Funding provided by NSF Division of Environmental Biology (NSF DEB) Award Number: DEB-1542240 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1542240</gmx:Anchor>
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&lt;p&gt;Marine oxygen deficient zones (ODZs) are waters that are functionally devoid of oxygen. Without oxygen, some microbes are capable of converting nitrogen in the water into N2 gas, which then leaves the ocean and enters the atmosphere. This loss of an important nutrient from the ocean has impacts on phytoplankton growth and marine food webs. While oxygen deficient zones occupy a very small percentage of the ocean, they account for as much as half of the oceanic loss of N as N2. Moreover, the size of these regions is predicted to expand during this century due to climate change. The microbes that are capable of producing N2 gas are extremely diverse, and use several different biochemical pathways to carry out this process. They may occur both free-floating in the water and attached to small particles that are suspended or sinking from the surface waters and providing them a carbon source. However the importance of these two lifestyles (free-living vs particle attached) in terms of contributions to N loss from the oceans is not well understood. This project will identify the major organisms that result in N2 gas production on both suspended and sinking particles, the chemical reactions they carry out, and the rates at which this occurs. This information will be used to improve global climate models to better predict rates of N loss in a future ocean. Elementary and middle school teachers enrolled in a Masters in Science for Science Teachers program will be involved in the project and the graduate students and post-doctoral researchers supported by the project will have opportunities to participate in their classrooms. Underserved populations will also be integrated into the research at the undergraduate and middle school level through a series of summer internships.&lt;/p&gt;
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&amp;lt;p&amp;gt;Some trap deployments functioned as simple sediment traps, and some deployments were combined trap and in situ incubators. The combined trap incubators consisted of upper and lower chambers. The material used to calculate fluxes reported here was collected from the upper chamber and was not incubated.&amp;amp;nbsp; Sinking material was collected in the lower incubation chamber, then isolated by closed a gate valve and injected with &amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N-NO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;-&amp;lt;/sup&amp;gt; . While the incubation occurred (in situ), sinking material was collected in an upper chamber that never encountered the spiked material. However, we do not report d&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N natural stable isotopes for the sediment trap material here due to proximity to the incubation.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;After every deployment, sediment trap material was filtered onto pre-combusted GF-75 filters (0.3 µm nominal pore size). To conform to community standards, zooplankton carcasses were not included in the measurements of carbon and nitrogen flux.&amp;amp;nbsp;Filter samples (particles only) were wafted with HCl overnight to remove carbonate, dried at 40°C, packed into silver foil cups, and sent to the University of Washington Isolab facility in the Department of Earth and Space Sciences (Seattle, WA) for C and N analysis utilizing an Costech elemental analyzer &amp;amp;nbsp;attached to an isotope ratio mass spectrometer (ThermoFinnegan MAT 253).&amp;lt;/p&amp;gt;</gco:CharacterString>
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** In the BCO-DMO data system missing data identifiers are displayed according to the format of data you access. For example, in csv files it will be blank (null) values. In Matlab .mat files it will be NaN values. When viewing data online at BCO-DMO, the missing value will be shown as blank (null) values.

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* Lat lon converted to decimal degrees (south and west are negative, degree symbols and directional NSEW removed)

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